Prupe.3G119200_v2.0.a1

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0001294
Physical Location & Seq
Forward (+)
10404628 .. 10405540
913 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G119200.1

Sequence Viewer

Length: 798 bp
ATGAAAAGAAAGGCAGAAATAAAAGTAGAAGTAAAAATAAAGAAGAAGAGGAATTGCAGGCGTAATCATGGAGTGAACTGGGTAAGAGGAGAGCTGATCGGAAGAGGAAGATATGGGTCTGTTCATCTTGCCACTTCCACGAAACCCATCACACGGTTTGGTCGTAAGCCTGAAATTATGGTGGTGAAATCGTCTAACTGGGTGCCATGTTTAAGGTCTGTTCAGCATGAGGCAGAGGTGCTCATCAAACTTAAGGGCTGCCCTTCTGTTATTGAGTGCTTTGGTGGACAATTCACCGCCGATGAGAATGGTGAGAAGGTCTACAATTTGTTCTTGGAATATGCCTCTGGCCTTGATCATCTGATCAAGAATTCCGATGGGGTTAAGCACATTCACCAGTGTGATTATGTTCACTGCGATTTGACCCCGGAGAACATTCTGCTTGTGCCTACCACCACAAGTTGTGGAAGTACTAGTTTGGTGGCGAAGATAGCCGATTTCGGATTGGCTAAGAGAACAAAAGAAAAGGATCAGCCCTCTGAAATTTGGTCTGTTGGTTGCATTGTGCTTGAGATGCTCATCGGGAAGCCCCCATGGGATACGCGTTTTTGTTGGAAGCCTCAGGATTTCCTGGACATGCTTGTTTTTGACTCTCCCAAGATTCCAGCTAAAATCTCAAAGGAGGGGAGGGATTTTCTAAAGATCTGCCTAGCCTTCAATCCCTGTAAAAGGTTAACTGCTGAAGAGCTCTTATCTCATCTGTTCGTTTGTGCAGTGAATACAACCAGAAGAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

266

Amino Acids

30.0

Weight (kDa)

9.23

Isoelectric Point (pI)

37.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 202
AccI GTMKAC 1 cut(s) 321
AccII CGCG 1 cut(s) 604
AciI CCGC 1 cut(s) 297
AclWI GGATC 1 cut(s) 537
AcsI RAATTY 2 cut(s) 370, 543
AcuI CTGAAG 1 cut(s) 762
AfaI GTAC 1 cut(s) 472
AfiI CCNNNNNNNGG 2 cut(s) 153, 729
AflII CTTAAG 1 cut(s) 251
AflIII ACRYGT 1 cut(s) 602
AgsI TTSAA 1 cut(s) 718
AhlI ACTAGT 1 cut(s) 473
AjnI CCWGG 1 cut(s) 630
AleI CACNNNNGTG 1 cut(s) 399
AluBI AGCT 3 cut(s) 94, 668, 748
AluI AGCT 3 cut(s) 94, 668, 748
Alw21I GWGCWC 2 cut(s) 243, 750
AlwI GGATC 1 cut(s) 537
AoxI GGCC 1 cut(s) 349
ApeKI GCWGC 1 cut(s) 258
ApoI RAATTY 2 cut(s) 370, 543
AsuC2I CCSGG 1 cut(s) 428
AsuHPI GGTGA 4 cut(s) 196, 286, 323, 386
AxyI CCTNAGG 1 cut(s) 621
BanI GGYRCC 1 cut(s) 202
BanII GRGCYC 1 cut(s) 750
Bbv12I GWGCWC 2 cut(s) 243, 750
BbvI GCAGC 1 cut(s) 245
BccI CCATC 2 cut(s) 155, 371
BciT130I CCWGG 1 cut(s) 632
BciVI GTATCC 1 cut(s) 592
BclI TGATCA 2 cut(s) 355, 363
BcnI CCSGG 1 cut(s) 428
BcuI ACTAGT 1 cut(s) 473
BfaI CTAG 2 cut(s) 474, 710
BfrI CTTAAG 1 cut(s) 251
BfuI GTATCC 1 cut(s) 592
BglII AGATCT 1 cut(s) 702
BisI GCNGC 1 cut(s) 259
BlsI GCNGC 1 cut(s) 260
BmcAI AGTACT 1 cut(s) 472
Bme1390I CCNGG 2 cut(s) 428, 632
BmiI GGNNCC 1 cut(s) 204
BmrFI CCNGG 2 cut(s) 428, 632
BmrI ACTGGG 2 cut(s) 88, 208
BmsI GCATC 1 cut(s) 564
BmuI ACTGGG 2 cut(s) 88, 208
BpuEI CTTGAG 1 cut(s) 590
BpuMI CCSGG 1 cut(s) 428
BsaBI GATNNNNATC 1 cut(s) 578
BsaJI CCNNGG 2 cut(s) 426, 593
Bsc4I CCNNNNNNNGG 2 cut(s) 153, 729
Bse1I ACTGG 3 cut(s) 83, 203, 397
Bse21I CCTNAGG 1 cut(s) 621
Bse8I GATNNNNATC 1 cut(s) 578
BseBI CCWGG 1 cut(s) 632
BseDI CCNNGG 2 cut(s) 426, 593
BseJI GATNNNNATC 1 cut(s) 578
BseLI CCNNNNNNNGG 2 cut(s) 153, 729
BseMII CTCAG 1 cut(s) 635
BseNI ACTGG 3 cut(s) 83, 203, 397
BseRI GAGGAG 1 cut(s) 102
BseXI GCAGC 1 cut(s) 245
BsgI GTGCAG 1 cut(s) 792
Bsh1236I CGCG 1 cut(s) 604
BshFI GGCC 1 cut(s) 351
BshNI GGYRCC 1 cut(s) 202
BsiHKAI GWGCWC 2 cut(s) 243, 750
BsiSI CCGG 1 cut(s) 428
BslI CCNNNNNNNGG 2 cut(s) 153, 729
BsnI GGCC 1 cut(s) 351
Bsp1286I GDGCHC 2 cut(s) 243, 750
Bsp143I GATC 5 cut(s) 96, 355, 363, 529, 702
Bsp19I CCATGG 1 cut(s) 593
BspACI CCGC 1 cut(s) 297
BspANI GGCC 1 cut(s) 351
BspCNI CTCAG 1 cut(s) 634
BspFNI CGCG 1 cut(s) 604
BspLI GGNNCC 1 cut(s) 204
BspPI GGATC 1 cut(s) 537
BspQI GCTCTTC 1 cut(s) 738
BspT107I GGYRCC 1 cut(s) 202
BspTI CTTAAG 1 cut(s) 251
BsrI ACTGG 3 cut(s) 83, 203, 397
BssECI CCNNGG 2 cut(s) 426, 593
BssMI GATC 5 cut(s) 96, 355, 363, 529, 702
BssT1I CCWWGG 1 cut(s) 593
Bst2UI CCWGG 1 cut(s) 632
Bst4CI ACNGT 1 cut(s) 156
Bst6I CTCTTC 3 cut(s) 41, 97, 738
BstAFI CTTAAG 1 cut(s) 251
BstC8I GCNNGC 1 cut(s) 59
BstDEI CTNAG 2 cut(s) 510, 621
BstDSI CCRYGG 1 cut(s) 593
BstENI CCTNNNNNAGG 1 cut(s) 727
BstFNI CGCG 1 cut(s) 604
BstKTI GATC 5 cut(s) 99, 358, 366, 532, 705
BstMBI GATC 5 cut(s) 96, 355, 363, 529, 702
BstMWI GCNNNNNNNGC 2 cut(s) 491, 574
BstNI CCWGG 1 cut(s) 632
BstNSI RCATGY 1 cut(s) 640
BstSCI CCNGG 2 cut(s) 426, 630
BstUI CGCG 1 cut(s) 604
BstV1I GCAGC 1 cut(s) 245
BstX2I RGATCY 1 cut(s) 702
BstYI RGATCY 1 cut(s) 702
Bsu36I CCTNAGG 1 cut(s) 621
BsuI GTATCC 1 cut(s) 592
BsuRI GGCC 1 cut(s) 351
BtgI CCRYGG 1 cut(s) 593
BtsI GCAGTG 2 cut(s) 412, 780
BtsIMutI CAGTG 3 cut(s) 404, 412, 780
Cac8I GCNNGC 1 cut(s) 59
Csp6I GTAC 1 cut(s) 471
CviAII CATG 5 cut(s) 68, 207, 227, 594, 637
CviQI GTAC 1 cut(s) 471
DdeI CTNAG 2 cut(s) 510, 621
DpnI GATC 5 cut(s) 98, 357, 365, 531, 704
DpnII GATC 5 cut(s) 96, 355, 363, 529, 702
Eam1104I CTCTTC 3 cut(s) 41, 97, 738
EarI CTCTTC 3 cut(s) 41, 97, 738
Ecl136II GAGCTC 1 cut(s) 748
Eco130I CCWWGG 1 cut(s) 593
Eco24I GRGCYC 1 cut(s) 750
Eco53kI GAGCTC 1 cut(s) 748
Eco57I CTGAAG 1 cut(s) 762
Eco81I CCTNAGG 1 cut(s) 621
EcoICRI GAGCTC 1 cut(s) 748
EcoNI CCTNNNNNAGG 1 cut(s) 727
EcoRI GAATTC 1 cut(s) 370
EcoRII CCWGG 1 cut(s) 630
EcoT14I CCWWGG 1 cut(s) 593
EcoT38I GRGCYC 1 cut(s) 750
ErhI CCWWGG 1 cut(s) 593
FaeI CATG 5 cut(s) 71, 210, 230, 597, 640
FatI CATG 5 cut(s) 67, 206, 226, 593, 636
FbaI TGATCA 2 cut(s) 355, 363
FblI GTMKAC 1 cut(s) 321
Fnu4HI GCNGC 1 cut(s) 259
FriOI GRGCYC 1 cut(s) 750
Fsp4HI GCNGC 1 cut(s) 259
FspBI CTAG 2 cut(s) 474, 710
GluI GCNGC 1 cut(s) 259
HaeIII GGCC 1 cut(s) 351
HapII CCGG 1 cut(s) 428
Hin1II CATG 5 cut(s) 71, 210, 230, 597, 640
HincII GTYRAC 1 cut(s) 735
HindII GTYRAC 1 cut(s) 735
HinfI GANTC 2 cut(s) 650, 661
HpaI GTTAAC 1 cut(s) 735
HpaII CCGG 1 cut(s) 428
HphI GGTGA 4 cut(s) 196, 286, 323, 386
Hpy166II GTNNAC 5 cut(s) 76, 287, 322, 412, 735
Hpy188I TCNGA 5 cut(s) 101, 363, 376, 503, 541
Hpy188III TCNNGA 3 cut(s) 367, 583, 623
Hpy8I GTNNAC 5 cut(s) 76, 287, 322, 412, 735
HpyAV CCTTC 3 cut(s) 273, 310, 724
HpyCH4III ACNGT 1 cut(s) 156
HpyCH4V TGCA 3 cut(s) 57, 561, 773
HpyF10VI GCNNNNNNNGC 2 cut(s) 491, 574
HpyF3I CTNAG 2 cut(s) 510, 621
Hsp92II CATG 5 cut(s) 71, 210, 230, 597, 640
Ksp22I TGATCA 2 cut(s) 355, 363
KspAI GTTAAC 1 cut(s) 735
Kzo9I GATC 5 cut(s) 96, 355, 363, 529, 702
LguI GCTCTTC 1 cut(s) 738
Lsp1109I GCAGC 1 cut(s) 245
LweI GCATC 1 cut(s) 564
MaeI CTAG 2 cut(s) 474, 710
MalI GATC 5 cut(s) 98, 357, 365, 531, 704
MboI GATC 5 cut(s) 96, 355, 363, 529, 702
MboII GAAGA 6 cut(s) 55, 58, 114, 120, 499, 755
MflI RGATCY 1 cut(s) 702
MhlI GDGCHC 2 cut(s) 243, 750
MluCI AATT 6 cut(s) 52, 174, 290, 325, 370, 543
MluI ACGCGT 1 cut(s) 602
MlyI GAGTC 1 cut(s) 644
MmeI TCCRAC 1 cut(s) 593
MseI TTAA 4 cut(s) 212, 252, 384, 734
MslI CAYNNNNRTG 1 cut(s) 399
MspCI CTTAAG 1 cut(s) 251
MspI CCGG 1 cut(s) 428
MspR9I CCNGG 2 cut(s) 428, 632
MvaI CCWGG 1 cut(s) 632
MvnI CGCG 1 cut(s) 604
MwoI GCNNNNNNNGC 2 cut(s) 491, 574
NciI CCSGG 1 cut(s) 428
NcoI CCATGG 1 cut(s) 593
NdeII GATC 5 cut(s) 96, 355, 363, 529, 702
NlaIII CATG 5 cut(s) 71, 210, 230, 597, 640
NlaIV GGNNCC 1 cut(s) 204
NspI RCATGY 1 cut(s) 640
OliI CACNNNNGTG 1 cut(s) 399
PciSI GCTCTTC 1 cut(s) 738
PcsI WCGNNNNNNNCGW 1 cut(s) 160
PfeI GAWTC 1 cut(s) 661
PfoI TCCNGGA 1 cut(s) 630
PkrI GCNGC 1 cut(s) 260
PleI GAGTC 1 cut(s) 644
PpsI GAGTC 1 cut(s) 644
Psp124BI GAGCTC 1 cut(s) 750
Psp6I CCWGG 1 cut(s) 630
PspGI CCWGG 1 cut(s) 630
PspN4I GGNNCC 1 cut(s) 204
PsuI RGATCY 1 cut(s) 702
RsaI GTAC 1 cut(s) 472
RsaNI GTAC 1 cut(s) 471
RseI CAYNNNNRTG 1 cut(s) 399
SacI GAGCTC 1 cut(s) 750
SapI GCTCTTC 1 cut(s) 738
SaqAI TTAA 4 cut(s) 212, 252, 384, 734
SatI GCNGC 1 cut(s) 259
Sau3AI GATC 5 cut(s) 96, 355, 363, 529, 702
ScaI AGTACT 1 cut(s) 472
SchI GAGTC 1 cut(s) 644
ScrFI CCNGG 2 cut(s) 428, 632
SduI GDGCHC 2 cut(s) 243, 750
SetI ASST 7 cut(s) 96, 218, 240, 321, 670, 734, 750
SfaNI GCATC 1 cut(s) 564
SmiMI CAYNNNNRTG 1 cut(s) 399
SmlI CTYRAG 2 cut(s) 251, 569
SmoI CTYRAG 2 cut(s) 251, 569
SpeI ACTAGT 1 cut(s) 473
Sse9I AATT 6 cut(s) 52, 174, 290, 325, 370, 543
SsiI CCGC 1 cut(s) 297
SspMI CTAG 2 cut(s) 474, 710
SstI GAGCTC 1 cut(s) 750
StyD4I CCNGG 2 cut(s) 426, 630
StyI CCWWGG 1 cut(s) 593
TaaI ACNGT 1 cut(s) 156
TasI AATT 6 cut(s) 52, 174, 290, 325, 370, 543
TatI WGTACW 1 cut(s) 470
TfiI GAWTC 1 cut(s) 661
Tru1I TTAA 4 cut(s) 212, 252, 384, 734
Tru9I TTAA 4 cut(s) 212, 252, 384, 734
TscAI CASTG 3 cut(s) 404, 419, 780
TseI GCWGC 1 cut(s) 258
TspDTI ATGAA 2 cut(s) 17, 113
TspRI CASTG 3 cut(s) 404, 419, 780
Vha464I CTTAAG 1 cut(s) 251
XagI CCTNNNNNAGG 1 cut(s) 727
XapI RAATTY 2 cut(s) 370, 543
XceI RCATGY 1 cut(s) 640
XmiI GTMKAC 1 cut(s) 321
XspI CTAG 2 cut(s) 474, 710
ZrmI AGTACT 1 cut(s) 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.