Rh6DG322100

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0001294
Physical Location & Seq
Forward (+)
52747109 .. 52748186
1078 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG322100.1

Sequence Viewer

Length: 594 bp
ATGGAGGATGAGGGCGAAGAGGTTTATAACTTGGCGTTGGAAGTTGCATGGGGAAGTCTTGCAAGGCTGATAAAGGTTTTCGGTTCTGGGTTGCCGGAATCTGATGTGCGGCGGTATACCTGTGACCTTCTTGAAGGAATTAGACACATCCACAAGTGTGGTTATGTCCACTGTGGCTTGAAGCCACAAAATATTATTCTTGTGGAAAATGAAGATGACGACGTCTATAGCTTCGTGGCCAAAGTGGCTGATTTGGGGTTGGCTAAGAAGAAGGATGAGGTTCAAGGAAGGTGGAGAGGCACACCCATGTACTTGTCTCCGGAGGCTATACTGTACAATGAACAAGAGGAGCCAAGTGATATTTGGGCCTTGGGGTGCATCGTTCTTGAGATGCTCACCGGAGAGTGCCCTCGGAACCATTTTTACGATCATGAGAGTGACACCCTCTACGTTGTTGATGGAATGGTGCCTAAAATCCCGGATACAATCTCAAGTCTTGCAAGGGATTTTCTAGATTGTTGCCTAGCCGAGGAAAGGTCCACAGCCGAAGAGCTTCTGTCTCATCCCTTTGTAGCAGAAGTTGTTAAACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

197

Amino Acids

22.24

Weight (kDa)

4.61

Isoelectric Point (pI)

46.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 8 - 191 5.5e-33 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 15 - 179 1.5e-15 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 27
AatII GACGTC 1 cut(s) 225
AccB1I GGYRCC 1 cut(s) 466
AccI GTMKAC 1 cut(s) 116
AccIII TCCGGA 1 cut(s) 319
AciI CCGC 2 cut(s) 109, 112
AcoI YGGCCR 1 cut(s) 237
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 2 cut(s) 311, 335
AfiI CCNNNNNNNGG 2 cut(s) 529, 534
AgsI TTSAA 3 cut(s) 134, 181, 284
AleI CACNNNNGTG 1 cut(s) 156
AluBI AGCT 2 cut(s) 231, 553
AluI AGCT 2 cut(s) 231, 553
Alw26I GTCTC 2 cut(s) 321, 564
Aor13HI TCCGGA 1 cut(s) 319
AoxI GGCC 2 cut(s) 237, 366
Asp700I GAANNNNTTC 1 cut(s) 552
AspS9I GGNCC 2 cut(s) 366, 537
AsuC2I CCSGG 1 cut(s) 479
AsuHPI GGTGA 1 cut(s) 388
AvaII GGWCC 1 cut(s) 537
BaeGI GKGCMC 1 cut(s) 410
BalI TGGCCA 1 cut(s) 239
BanI GGYRCC 1 cut(s) 466
BccI CCATC 1 cut(s) 452
BciVI GTATCC 1 cut(s) 475
BcnI CCSGG 1 cut(s) 479
BcoDI GTCTC 2 cut(s) 321, 564
BfaI CTAG 2 cut(s) 512, 524
BfmI CTRYAG 1 cut(s) 226
BfuI GTATCC 1 cut(s) 475
BglI GCCNNNNNGGC 1 cut(s) 245
BisI GCNGC 1 cut(s) 110
BlsI GCNGC 1 cut(s) 111
Bme1390I CCNGG 1 cut(s) 479
Bme18I GGWCC 1 cut(s) 537
BmgT120I GGNCC 2 cut(s) 366, 537
BmiI GGNNCC 3 cut(s) 351, 416, 468
BmrFI CCNGG 1 cut(s) 479
BmsI GCATC 2 cut(s) 381, 387
BpuEI CTTGAG 2 cut(s) 407, 475
BpuMI CCSGG 1 cut(s) 479
BsaHI GRCGYC 1 cut(s) 222
BsaJI CCNNGG 3 cut(s) 369, 410, 528
BsaWI WCCGGW 2 cut(s) 319, 398
Bsc4I CCNNNNNNNGG 2 cut(s) 529, 534
BseAI TCCGGA 1 cut(s) 319
BseDI CCNNGG 3 cut(s) 369, 410, 528
BseGI GGATG 4 cut(s) 13, 147, 280, 562
BseLI CCNNNNNNNGG 2 cut(s) 529, 534
BseRI GAGGAG 1 cut(s) 362
BseSI GKGCMC 1 cut(s) 410
BshFI GGCC 2 cut(s) 239, 368
BshNI GGYRCC 1 cut(s) 466
BsiSI CCGG 4 cut(s) 95, 320, 399, 479
BslI CCNNNNNNNGG 2 cut(s) 529, 534
BsmAI GTCTC 2 cut(s) 321, 564
BsnI GGCC 2 cut(s) 239, 368
Bsp1286I GDGCHC 1 cut(s) 410
Bsp13I TCCGGA 1 cut(s) 319
Bsp1407I TGTACA 1 cut(s) 333
Bsp143I GATC 1 cut(s) 427
BspACI CCGC 2 cut(s) 109, 112
BspANI GGCC 2 cut(s) 239, 368
BspEI TCCGGA 1 cut(s) 319
BspHI TCATGA 1 cut(s) 430
BspLI GGNNCC 3 cut(s) 351, 416, 468
BspQI GCTCTTC 1 cut(s) 543
BspT107I GGYRCC 1 cut(s) 466
BsrGI TGTACA 1 cut(s) 333
BssECI CCNNGG 3 cut(s) 369, 410, 528
BssMI GATC 1 cut(s) 427
BssNAI GTATAC 1 cut(s) 117
BssNI GRCGYC 1 cut(s) 222
BssT1I CCWWGG 1 cut(s) 369
Bst1107I GTATAC 1 cut(s) 117
Bst4CI ACNGT 2 cut(s) 173, 333
Bst6I CTCTTC 2 cut(s) 12, 543
BstACI GRCGYC 1 cut(s) 222
BstAUI TGTACA 1 cut(s) 333
BstDEI CTNAG 1 cut(s) 264
BstENI CCTNNNNNAGG 1 cut(s) 527
BstF5I GGATG 4 cut(s) 13, 147, 280, 562
BstKTI GATC 1 cut(s) 430
BstMAI GTCTC 2 cut(s) 321, 564
BstMBI GATC 1 cut(s) 427
BstMWI GCNNNNNNNGC 1 cut(s) 245
BstSCI CCNGG 1 cut(s) 477
BstSFI CTRYAG 1 cut(s) 226
BstSLI GKGCMC 1 cut(s) 410
BstXI CCANNNNNNTGG 1 cut(s) 158
BstZ17I GTATAC 1 cut(s) 117
BsuI GTATCC 1 cut(s) 475
BsuRI GGCC 2 cut(s) 239, 368
BtsCI GGATG 4 cut(s) 13, 147, 280, 562
BtsIMutI CAGTG 2 cut(s) 169, 589
CciI TCATGA 1 cut(s) 430
Cfr13I GGNCC 2 cut(s) 366, 537
Csp6I GTAC 2 cut(s) 310, 334
CspCI CAANNNNNGTGG 2 cut(s) 272, 307
CviAII CATG 3 cut(s) 48, 307, 431
CviQI GTAC 2 cut(s) 310, 334
DdeI CTNAG 1 cut(s) 264
DpnI GATC 1 cut(s) 429
DpnII GATC 1 cut(s) 427
EaeI YGGCCR 1 cut(s) 237
Eam1104I CTCTTC 2 cut(s) 12, 543
EarI CTCTTC 2 cut(s) 12, 543
Eco130I CCWWGG 1 cut(s) 369
Eco47I GGWCC 1 cut(s) 537
EcoNI CCTNNNNNAGG 1 cut(s) 527
EcoT14I CCWWGG 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 369
FaeI CATG 3 cut(s) 51, 310, 434
FaiI YATR 8 cut(s) 27, 49, 117, 165, 228, 308, 329, 432
FatI CATG 3 cut(s) 47, 306, 430
FblI GTMKAC 1 cut(s) 116
Fnu4HI GCNGC 1 cut(s) 110
FokI GGATG 4 cut(s) 20, 134, 287, 549
Fsp4HI GCNGC 1 cut(s) 110
FspBI CTAG 2 cut(s) 512, 524
GluI GCNGC 1 cut(s) 110
HaeIII GGCC 2 cut(s) 239, 368
HapII CCGG 4 cut(s) 95, 320, 399, 479
Hin1I GRCGYC 1 cut(s) 222
Hin1II CATG 3 cut(s) 51, 310, 434
HinfI GANTC 1 cut(s) 98
HpaII CCGG 4 cut(s) 95, 320, 399, 479
HphI GGTGA 1 cut(s) 388
Hpy166II GTNNAC 3 cut(s) 117, 169, 540
Hpy188I TCNGA 2 cut(s) 103, 414
Hpy188III TCNNGA 5 cut(s) 131, 320, 386, 431, 512
Hpy8I GTNNAC 3 cut(s) 117, 169, 540
Hpy99I CGWCG 1 cut(s) 224
HpyAV CCTTC 4 cut(s) 128, 137, 265, 282
HpyCH4III ACNGT 2 cut(s) 173, 333
HpyCH4IV ACGT 2 cut(s) 222, 450
HpyCH4V TGCA 4 cut(s) 47, 62, 378, 500
HpyF10VI GCNNNNNNNGC 1 cut(s) 245
HpyF3I CTNAG 1 cut(s) 264
HpySE526I ACGT 2 cut(s) 222, 450
Hsp92I GRCGYC 1 cut(s) 222
Hsp92II CATG 3 cut(s) 51, 310, 434
Kpn2I TCCGGA 1 cut(s) 319
Kzo9I GATC 1 cut(s) 427
LguI GCTCTTC 1 cut(s) 543
LmnI GCTCC 1 cut(s) 349
LpnPI CCDG 6 cut(s) 72, 108, 133, 333, 412, 492
LweI GCATC 2 cut(s) 381, 387
MaeI CTAG 2 cut(s) 512, 524
MaeII ACGT 2 cut(s) 222, 450
MaeIII GTNAC 2 cut(s) 122, 437
MalI GATC 1 cut(s) 429
MboI GATC 1 cut(s) 427
MboII GAAGA 4 cut(s) 29, 224, 280, 560
MhlI GDGCHC 1 cut(s) 410
MlsI TGGCCA 1 cut(s) 239
MluCI AATT 1 cut(s) 138
MluNI TGGCCA 1 cut(s) 239
MmeI TCCRAC 1 cut(s) 18
MnlI CCTC 9 cut(s) 4, 13, 271, 290, 316, 340, 420, 455, 523
Mox20I TGGCCA 1 cut(s) 239
MroI TCCGGA 1 cut(s) 319
MroXI GAANNNNTTC 1 cut(s) 552
MscI TGGCCA 1 cut(s) 239
MseI TTAA 1 cut(s) 585
MslI CAYNNNNRTG 3 cut(s) 156, 305, 435
Msp20I TGGCCA 1 cut(s) 239
MspI CCGG 4 cut(s) 95, 320, 399, 479
MspR9I CCNGG 1 cut(s) 479
MwoI GCNNNNNNNGC 1 cut(s) 245
NciI CCSGG 1 cut(s) 479
NdeII GATC 1 cut(s) 427
NlaIII CATG 3 cut(s) 51, 310, 434
NlaIV GGNNCC 3 cut(s) 351, 416, 468
NmeAIII GCCGAG 1 cut(s) 553
NmuCI GTSAC 2 cut(s) 122, 437
OliI CACNNNNGTG 1 cut(s) 156
PagI TCATGA 1 cut(s) 430
PciSI GCTCTTC 1 cut(s) 543
PdmI GAANNNNTTC 1 cut(s) 552
PfeI GAWTC 1 cut(s) 98
PflFI GACNNNGTC 1 cut(s) 221
PfoI TCCNGGA 1 cut(s) 477
PkrI GCNGC 1 cut(s) 111
PsiI TTATAA 1 cut(s) 27
PspN4I GGNNCC 3 cut(s) 351, 416, 468
PspPI GGNCC 2 cut(s) 366, 537
PsrI GAACNNNNNNTAC 2 cut(s) 407, 439
PsyI GACNNNGTC 1 cut(s) 221
RsaI GTAC 2 cut(s) 311, 335
RsaNI GTAC 2 cut(s) 310, 334
RseI CAYNNNNRTG 3 cut(s) 156, 305, 435
SapI GCTCTTC 1 cut(s) 543
SaqAI TTAA 1 cut(s) 585
SatI GCNGC 1 cut(s) 110
Sau3AI GATC 1 cut(s) 427
Sau96I GGNCC 2 cut(s) 366, 537
ScrFI CCNGG 1 cut(s) 479
SduI GDGCHC 1 cut(s) 410
SfaNI GCATC 2 cut(s) 381, 387
SfcI CTRYAG 1 cut(s) 226
SinI GGWCC 1 cut(s) 537
SmiMI CAYNNNNRTG 3 cut(s) 156, 305, 435
SmlI CTYRAG 2 cut(s) 386, 490
SmoI CTYRAG 2 cut(s) 386, 490
Sse9I AATT 1 cut(s) 138
SsiI CCGC 2 cut(s) 109, 112
SspI AATATT 1 cut(s) 193
SspMI CTAG 2 cut(s) 512, 524
StyD4I CCNGG 1 cut(s) 477
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 2 cut(s) 173, 333
TaiI ACGT 2 cut(s) 225, 453
TasI AATT 1 cut(s) 138
TatI WGTACW 2 cut(s) 309, 333
TauI GCSGC 1 cut(s) 112
TfiI GAWTC 1 cut(s) 98
Tru1I TTAA 1 cut(s) 585
Tru9I TTAA 1 cut(s) 585
TscAI CASTG 1 cut(s) 176
TseFI GTSAC 2 cut(s) 122, 437
Tsp45I GTSAC 2 cut(s) 122, 437
TspDTI ATGAA 2 cut(s) 225, 354
TspRI CASTG 1 cut(s) 176
Tth111I GACNNNGTC 1 cut(s) 221
VpaK11BI GGWCC 1 cut(s) 537
XagI CCTNNNNNAGG 1 cut(s) 527
XbaI TCTAGA 1 cut(s) 511
XcmI CCANNNNNNNNNTGG 1 cut(s) 360
XmiI GTMKAC 1 cut(s) 116
XmnI GAANNNNTTC 1 cut(s) 552
XspI CTAG 2 cut(s) 512, 524
ZraI GACGTC 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.