FvH4_4g24730

Belongs to the Orn Lys Arg decarboxylase class-II family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000734
Physical Location & Seq
Reverse (-)
26762430 .. 26763853
1424 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g24730.t1

Sequence Viewer

Length: 1269 bp
ATGGAGTCCTGCAATGTAAGTGCACCAGATCAGGTCGTGAGCAAAAACAGTCTGGGTGATGATGAATGTTTCATGCAGAAAATCATTTCGTCCATCAAGAAACTGCAGCAAATTGAGGAAGCATTTTACGTCCTGGATTTAGGTCTTGTAAGCAATCTCATGGAGACATGGAAACGTAACCTTCCAATGGTCCAACCTTTCTACGCCGTCAAGTGCAACCCTCACCCGGATCTCCTTGCCACCTTGGTAGCTCAAGGTTCAAGTTTCGACTGTGCAAGTGCGTCTGAGATCGAGTCCATTTTGGCTCTCGGAGTTTCGCCAGATCGCATCATCTATGCTAATCCCTGCAAAGCAAGCTCTCACATCAAGTACGCTGCTGAAGTTGGTGTTAACCTAACCACTTTCGACTCGGTAGATGAGGTTGAAAAGATCGGAAAATGGCACCCGAAATGTTCTTTACTGATCCGCATCAAAGCTCCGGACGAGAGCGGATCCCAGTGGCCACTGGGTTCCAAGTTCGGTGCTCTCCCTGAAGAAGTGTCTCCGCTCCTCCAAGCCGCTCAAACTGCAGGACTCAAAGTTTCCGGGGTTTCATTTCACATAGGAAGCAGGTCACACAATTTTCAAGCATACAAAAAAGCTATAGAGGCAGCTAAGGTGGTATTCCAGGAGGCGGCCGGGCTCTGCATGCCTCCCATGCATGTGTTGAACATCGGCGGTGGCTTCCTGTCCGAGCCATCGTTCTTTGAAGCTGCGGCGGCAGCTGTGAAAACCGCTGTGGAGACGAATTTTTCAGAGGAGGAGCGACGCAGCTTAACGGTGATCGCCGAGCCGGGTCGGTTCTTTGCAGAGACTCCATTCACTCTTGCCACCAATATCATCGGGAAGCGTGTGAGAGGGGAGCTCAGACAATACTGGATCAACGAAGGGTTTTACGGGTCCATGAGGCTTGCGGAACCTAAAACCCTACTTGAATCGTTCATTGCTCTGAAATTCATGTCCAAGTCCAATAACAGTGACGATGACATCGATAAGAATCCAGTTCTAGTATTGATGGTATGTGACAAAGATAGTGGTGGTGCTCGCACTTACAGTTCGACTGTGTTTGGTCCGACGTGTGATTCCGGTGACAAGGTGTTGGAGGGTGAGTTACCAGAACTGGAAGTGAATAATTGGCTGGTGTTTCGCAACATGGGTGCTTATACGTCGGCGTGCAGCAACAACTTCAATGGTTTTGGCTCCGCCAAGATCACCTATCTTACAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko00480 ko01100 ko01110 ko01130 map00330 map00480 map01100 map01110 map01130
Pfam Domains
Protein Families
Orn_Arg_deC_N Orn_DAP_Arg_deC

Protein Analysis

423

Amino Acids

46.05

Weight (kDa)

5.28

Isoelectric Point (pI)

50.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Orn_DAP_Arg_deC PF00278 44 - 399 3.1e-12 Pyridoxal-dependent decarboxylase, C-terminal sheet domain
Orn_Arg_deC_N PF02784 50 - 283 3e-68 Pyridoxal-dependent decarboxylase, pyridoxal binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 600
AccB1I GGYRCC 1 cut(s) 441
AccBSI CCGCTC 3 cut(s) 489, 547, 560
AccIII TCCGGA 1 cut(s) 478
AclWI GGATC 5 cut(s) 237, 457, 486, 499, 926
AcoI YGGCCR 2 cut(s) 500, 675
AcsI RAATTY 2 cut(s) 787, 992
AcuI CTGAAG 2 cut(s) 399, 552
AfaI GTAC 1 cut(s) 371
AfiI CCNNNNNNNGG 3 cut(s) 187, 226, 673
AflIII ACRYGT 1 cut(s) 1115
AgsI TTSAA 7 cut(s) 261, 425, 626, 709, 749, 974, 1228
AjiI CACGTC 1 cut(s) 1116
AjnI CCWGG 2 cut(s) 132, 666
AluBI AGCT 9 cut(s) 251, 357, 476, 641, 653, 752, 764, 813, 904
AluI AGCT 9 cut(s) 251, 357, 476, 641, 653, 752, 764, 813, 904
Alw21I GWGCWC 4 cut(s) 25, 526, 906, 1084
Alw26I GTCTC 4 cut(s) 158, 546, 776, 845
Alw44I GTGCAC 1 cut(s) 21
AlwI GGATC 5 cut(s) 237, 457, 486, 499, 926
Aor13HI TCCGGA 1 cut(s) 478
AoxI GGCC 2 cut(s) 500, 675
ApaLI GTGCAC 1 cut(s) 21
ApeKI GCWGC 7 cut(s) 106, 374, 650, 752, 761, 810, 1215
ApoI RAATTY 2 cut(s) 787, 992
AspS9I GGNCC 3 cut(s) 190, 939, 1109
AsuC2I CCSGG 4 cut(s) 227, 586, 679, 834
AsuHPI GGTGA 6 cut(s) 68, 215, 832, 1139, 1157, 1243
AvaII GGWCC 3 cut(s) 190, 939, 1109
BaeGI GKGCMC 1 cut(s) 25
BalI TGGCCA 1 cut(s) 502
BamHI GGATCC 1 cut(s) 491
BanI GGYRCC 1 cut(s) 441
BanII GRGCYC 2 cut(s) 684, 906
Bbv12I GWGCWC 4 cut(s) 25, 526, 906, 1084
BbvI GCAGC 7 cut(s) 118, 361, 662, 739, 773, 822, 1227
BccI CCATC 3 cut(s) 101, 745, 1048
BceAI ACGGC 1 cut(s) 191
BciT130I CCWGG 2 cut(s) 134, 668
BcnI CCSGG 4 cut(s) 227, 586, 679, 834
BcoDI GTCTC 4 cut(s) 158, 546, 776, 845
BfaI CTAG 1 cut(s) 1046
BfmI CTRYAG 3 cut(s) 104, 567, 642
BfuAI ACCTGC 1 cut(s) 600
Bme1390I CCNGG 6 cut(s) 134, 227, 586, 668, 679, 834
Bme18I GGWCC 3 cut(s) 190, 939, 1109
BmgBI CACGTC 1 cut(s) 1116
BmgT120I GGNCC 3 cut(s) 190, 939, 1109
BmiI GGNNCC 6 cut(s) 443, 493, 511, 940, 957, 1240
BmrFI CCNGG 6 cut(s) 134, 227, 586, 668, 679, 834
BmrI ACTGGG 2 cut(s) 490, 515
BmsI GCATC 2 cut(s) 336, 477
BmuI ACTGGG 2 cut(s) 490, 515
BplI GAGNNNNNCTC 2 cut(s) 888, 920
Bpu10I CCTNAGC 1 cut(s) 654
BpuEI CTTGAG 1 cut(s) 237
BpuMI CCSGG 4 cut(s) 227, 586, 679, 834
Bsa29I ATCGAT 1 cut(s) 1029
BsaBI GATNNNNATC 2 cut(s) 467, 1035
BsaJI CCNNGG 2 cut(s) 243, 585
BsaWI WCCGGW 2 cut(s) 478, 1124
BsaXI ACNNNNNCTCC 2 cut(s) 1133, 1163
Bsc4I CCNNNNNNNGG 3 cut(s) 187, 226, 673
Bse1I ACTGG 5 cut(s) 496, 510, 920, 1040, 1164
Bse3DI GCAATG 2 cut(s) 19, 981
Bse8I GATNNNNATC 2 cut(s) 467, 1035
BseAI TCCGGA 1 cut(s) 478
BseBI CCWGG 2 cut(s) 134, 668
BseCI ATCGAT 1 cut(s) 1029
BseDI CCNNGG 2 cut(s) 243, 585
BseJI GATNNNNATC 2 cut(s) 467, 1035
BseLI CCNNNNNNNGG 3 cut(s) 187, 226, 673
BseMI GCAATG 2 cut(s) 19, 981
BseMII CTCAG 2 cut(s) 276, 919
BseNI ACTGG 5 cut(s) 496, 510, 920, 1040, 1164
BseRI GAGGAG 3 cut(s) 539, 812, 815
BseSI GKGCMC 1 cut(s) 25
BseX3I CGGCCG 1 cut(s) 675
BseXI GCAGC 7 cut(s) 118, 361, 662, 739, 773, 822, 1227
BsgI GTGCAG 1 cut(s) 1234
Bsh1285I CGRYCG 1 cut(s) 678
BshFI GGCC 2 cut(s) 502, 677
BshNI GGYRCC 1 cut(s) 441
BshVI ATCGAT 1 cut(s) 1029
BsiEI CGRYCG 1 cut(s) 678
BsiHKAI GWGCWC 4 cut(s) 25, 526, 906, 1084
BsiSI CCGG 6 cut(s) 227, 479, 585, 678, 833, 1125
BslI CCNNNNNNNGG 3 cut(s) 187, 226, 673
BsmAI GTCTC 4 cut(s) 158, 546, 776, 845
BsmBI CGTCTC 1 cut(s) 776
BsnI GGCC 2 cut(s) 502, 677
Bsp1286I GDGCHC 5 cut(s) 25, 526, 684, 906, 1084
Bsp13I TCCGGA 1 cut(s) 478
BspANI GGCC 2 cut(s) 502, 677
BspCNI CTCAG 2 cut(s) 277, 918
BspDI ATCGAT 1 cut(s) 1029
BspEI TCCGGA 1 cut(s) 478
BspLI GGNNCC 6 cut(s) 443, 493, 511, 940, 957, 1240
BspMAI CTGCAG 2 cut(s) 108, 571
BspMI ACCTGC 1 cut(s) 600
BspPI GGATC 5 cut(s) 237, 457, 486, 499, 926
BspT107I GGYRCC 1 cut(s) 441
BsrBI CCGCTC 3 cut(s) 489, 547, 560
BsrDI GCAATG 2 cut(s) 19, 981
BsrI ACTGG 5 cut(s) 496, 510, 920, 1040, 1164
BssECI CCNNGG 2 cut(s) 243, 585
BssT1I CCWWGG 1 cut(s) 243
Bst2UI CCWGG 2 cut(s) 134, 668
Bst4CI ACNGT 6 cut(s) 50, 272, 820, 1016, 1094, 1102
BstC8I GCNNGC 5 cut(s) 355, 689, 951, 1084, 1213
BstDEI CTNAG 3 cut(s) 285, 654, 905
BstMAI GTCTC 4 cut(s) 158, 546, 776, 845
BstMCI CGRYCG 1 cut(s) 678
BstMWI GCNNNNNNNGC 7 cut(s) 354, 566, 647, 688, 697, 758, 761
BstNI CCWGG 2 cut(s) 134, 668
BstNSI RCATGY 2 cut(s) 691, 704
BstSCI CCNGG 6 cut(s) 132, 225, 584, 666, 677, 832
BstSFI CTRYAG 3 cut(s) 104, 567, 642
BstSLI GKGCMC 1 cut(s) 25
BstV1I GCAGC 7 cut(s) 118, 361, 662, 739, 773, 822, 1227
BstX2I RGATCY 2 cut(s) 229, 491
BstYI RGATCY 2 cut(s) 229, 491
BstZI CGGCCG 1 cut(s) 675
Bsu15I ATCGAT 1 cut(s) 1029
BsuRI GGCC 2 cut(s) 502, 677
BsuTUI ATCGAT 1 cut(s) 1029
BtrI CACGTC 1 cut(s) 1116
BtsIMutI CAGTG 3 cut(s) 503, 503, 1021
BveI ACCTGC 1 cut(s) 600
Cac8I GCNNGC 5 cut(s) 355, 689, 951, 1084, 1213
Cfr13I GGNCC 3 cut(s) 190, 939, 1109
ClaI ATCGAT 1 cut(s) 1029
CseI GACGC 2 cut(s) 270, 816
Csp6I GTAC 1 cut(s) 370
CspCI CAANNNNNGTGG 2 cut(s) 1054, 1089
CviAII CATG 9 cut(s) 73, 160, 168, 688, 697, 701, 943, 997, 1192
CviQI GTAC 1 cut(s) 370
DdeI CTNAG 3 cut(s) 285, 654, 905
EaeI YGGCCR 2 cut(s) 500, 675
EagI CGGCCG 1 cut(s) 675
EciI GGCGGA 1 cut(s) 1231
Ecl136II GAGCTC 1 cut(s) 904
EclXI CGGCCG 1 cut(s) 675
Eco130I CCWWGG 1 cut(s) 243
Eco24I GRGCYC 2 cut(s) 684, 906
Eco47I GGWCC 3 cut(s) 190, 939, 1109
Eco52I CGGCCG 1 cut(s) 675
Eco53kI GAGCTC 1 cut(s) 904
Eco57I CTGAAG 2 cut(s) 399, 552
EcoICRI GAGCTC 1 cut(s) 904
EcoRII CCWGG 2 cut(s) 132, 666
EcoT14I CCWWGG 1 cut(s) 243
EcoT22I ATGCAT 1 cut(s) 702
EcoT38I GRGCYC 2 cut(s) 684, 906
ErhI CCWWGG 1 cut(s) 243
Esp3I CGTCTC 1 cut(s) 776
FaeI CATG 9 cut(s) 76, 163, 171, 691, 700, 704, 946, 1000, 1195
FatI CATG 9 cut(s) 72, 159, 167, 687, 696, 700, 942, 996, 1191
FriOI GRGCYC 2 cut(s) 684, 906
FspBI CTAG 1 cut(s) 1046
HaeIII GGCC 2 cut(s) 502, 677
HapII CCGG 6 cut(s) 227, 479, 585, 678, 833, 1125
HgaI GACGC 2 cut(s) 270, 816
Hin1II CATG 9 cut(s) 76, 163, 171, 691, 700, 704, 946, 1000, 1195
HincII GTYRAC 1 cut(s) 391
HindII GTYRAC 1 cut(s) 391
HinfI GANTC 8 cut(s) 5, 293, 407, 573, 853, 974, 1036, 1121
HpaI GTTAAC 1 cut(s) 391
HpaII CCGG 6 cut(s) 227, 479, 585, 678, 833, 1125
HphI GGTGA 6 cut(s) 68, 215, 832, 1139, 1157, 1243
Hpy166II GTNNAC 2 cut(s) 23, 391
Hpy188I TCNGA 8 cut(s) 286, 311, 434, 733, 796, 908, 990, 1113
Hpy188III TCNNGA 4 cut(s) 37, 97, 479, 883
Hpy8I GTNNAC 2 cut(s) 23, 391
Hpy99I CGWCG 3 cut(s) 810, 1117, 1210
HpyAV CCTTC 2 cut(s) 191, 920
HpyCH4III ACNGT 6 cut(s) 50, 272, 820, 1016, 1094, 1102
HpyCH4IV ACGT 4 cut(s) 129, 175, 1115, 1205
HpyF10VI GCNNNNNNNGC 7 cut(s) 354, 566, 647, 688, 697, 758, 761
HpyF3I CTNAG 3 cut(s) 285, 654, 905
HpySE526I ACGT 4 cut(s) 129, 175, 1115, 1205
Hsp92II CATG 9 cut(s) 76, 163, 171, 691, 700, 704, 946, 1000, 1195
Kpn2I TCCGGA 1 cut(s) 478
KspAI GTTAAC 1 cut(s) 391
LmnI GCTCC 5 cut(s) 481, 552, 802, 901, 1244
Lsp1109I GCAGC 7 cut(s) 118, 361, 662, 739, 773, 822, 1227
LweI GCATC 2 cut(s) 336, 477
MaeI CTAG 1 cut(s) 1046
MaeII ACGT 4 cut(s) 129, 175, 1115, 1205
MaeIII GTNAC 6 cut(s) 176, 612, 1016, 1061, 1127, 1149
MbiI CCGCTC 3 cut(s) 489, 547, 560
MboII GAAGA 1 cut(s) 545
MflI RGATCY 2 cut(s) 229, 491
MhlI GDGCHC 5 cut(s) 25, 526, 684, 906, 1084
MlsI TGGCCA 1 cut(s) 502
MluCI AATT 5 cut(s) 111, 619, 787, 992, 1171
MluNI TGGCCA 1 cut(s) 502
MlyI GAGTC 5 cut(s) 14, 302, 401, 567, 847
MmeI TCCRAC 3 cut(s) 217, 1119, 1136
Mox20I TGGCCA 1 cut(s) 502
Mph1103I ATGCAT 1 cut(s) 702
MroI TCCGGA 1 cut(s) 478
MscI TGGCCA 1 cut(s) 502
MseI TTAA 2 cut(s) 390, 815
MslI CAYNNNNRTG 1 cut(s) 701
Msp20I TGGCCA 1 cut(s) 502
MspA1I CMGCKG 2 cut(s) 764, 776
MspI CCGG 6 cut(s) 227, 479, 585, 678, 833, 1125
MspR9I CCNGG 6 cut(s) 134, 227, 586, 668, 679, 834
MvaI CCWGG 2 cut(s) 134, 668
MwoI GCNNNNNNNGC 7 cut(s) 354, 566, 647, 688, 697, 758, 761
NciI CCSGG 4 cut(s) 227, 586, 679, 834
NlaIII CATG 9 cut(s) 76, 163, 171, 691, 700, 704, 946, 1000, 1195
NlaIV GGNNCC 6 cut(s) 443, 493, 511, 940, 957, 1240
NmeAIII GCCGAG 1 cut(s) 853
NmuCI GTSAC 4 cut(s) 612, 1016, 1061, 1127
NsiI ATGCAT 1 cut(s) 702
NspI RCATGY 2 cut(s) 691, 704
PaeI GCATGC 1 cut(s) 691
PcsI WCGNNNNNNNCGW 1 cut(s) 1026
PfeI GAWTC 3 cut(s) 974, 1036, 1121
PfoI TCCNGGA 2 cut(s) 132, 666
PleI GAGTC 5 cut(s) 13, 301, 401, 567, 847
PpsI GAGTC 5 cut(s) 13, 301, 401, 567, 847
Psp124BI GAGCTC 1 cut(s) 906
Psp6I CCWGG 2 cut(s) 132, 666
PspGI CCWGG 2 cut(s) 132, 666
PspN4I GGNNCC 6 cut(s) 443, 493, 511, 940, 957, 1240
PspPI GGNCC 3 cut(s) 190, 939, 1109
PstI CTGCAG 2 cut(s) 108, 571
PsuI RGATCY 2 cut(s) 229, 491
PvuII CAGCTG 1 cut(s) 764
RsaI GTAC 1 cut(s) 371
RsaNI GTAC 1 cut(s) 370
RseI CAYNNNNRTG 1 cut(s) 701
SacI GAGCTC 1 cut(s) 906
SaqAI TTAA 2 cut(s) 390, 815
Sau96I GGNCC 3 cut(s) 190, 939, 1109
SchI GAGTC 5 cut(s) 14, 302, 401, 567, 847
ScrFI CCNGG 6 cut(s) 134, 227, 586, 668, 679, 834
SduI GDGCHC 5 cut(s) 25, 526, 684, 906, 1084
SfaNI GCATC 2 cut(s) 336, 477
SfcI CTRYAG 3 cut(s) 104, 567, 642
SinI GGWCC 3 cut(s) 190, 939, 1109
SmiMI CAYNNNNRTG 1 cut(s) 701
SmlI CTYRAG 1 cut(s) 252
SmoI CTYRAG 1 cut(s) 252
SphI GCATGC 1 cut(s) 691
Sse9I AATT 5 cut(s) 111, 619, 787, 992, 1171
SspMI CTAG 1 cut(s) 1046
SstI GAGCTC 1 cut(s) 906
StyD4I CCNGG 6 cut(s) 132, 225, 584, 666, 677, 832
StyI CCWWGG 1 cut(s) 243
TaaI ACNGT 6 cut(s) 50, 272, 820, 1016, 1094, 1102
TaiI ACGT 4 cut(s) 132, 178, 1118, 1208
TaqI TCGA 5 cut(s) 267, 291, 405, 1029, 1097
TasI AATT 5 cut(s) 111, 619, 787, 992, 1171
TauI GCSGC 4 cut(s) 560, 677, 758, 761
TfiI GAWTC 3 cut(s) 974, 1036, 1121
Tru1I TTAA 2 cut(s) 390, 815
Tru9I TTAA 2 cut(s) 390, 815
TscAI CASTG 3 cut(s) 503, 510, 1021
TseFI GTSAC 4 cut(s) 612, 1016, 1061, 1127
TseI GCWGC 7 cut(s) 106, 374, 650, 752, 761, 810, 1215
Tsp45I GTSAC 4 cut(s) 612, 1016, 1061, 1127
TspDTI ATGAA 5 cut(s) 61, 78, 582, 970, 985
TspRI CASTG 3 cut(s) 503, 510, 1021
VneI GTGCAC 1 cut(s) 21
VpaK11BI GGWCC 3 cut(s) 190, 939, 1109
XapI RAATTY 2 cut(s) 787, 992
XceI RCATGY 2 cut(s) 691, 704
XspI CTAG 1 cut(s) 1046
Zsp2I ATGCAT 1 cut(s) 702
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.