FvH4_4g27890

Belongs to the Orn Lys Arg decarboxylase class-II family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000734
Physical Location & Seq
Forward (+)
28677089 .. 28678962
1874 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g27890.t1

Sequence Viewer

Length: 1356 bp
ATGTTAGCTTTGATAACTCTTGGCTCTCAACAACTGGAACGAGTCTTATTTGTCTTGTTTTCATTTGGCCGGAGTCATCACCAACATCACAGCATGGAGTCCTGCAATGTAAGTGCACCAGATCAGGTCGTAAGCAAAAACAGTCTGGGTGATGATGAATGTTTCATGCAGAAAATCATTTCGTCCATCAAGAAACTGCAGCAAATTGAGGAAGCATTTTACGTCCTGGATTTAGGTCTTGTAAGCAACCTCATGGAGACATGGAAACGTAACCTTCCAATGGTCCAACCTTTCTACTCCGTCAAGTGCAACCCTCACCCGGATCTTCTTGCCACGTTGGTAGCTCAAGGTTCAAATTTCGACTGTGCAAGTCCGTCTGAGATCAAGTCCATTTTGGCTCTAGGAGTTTCGCCGGATCGCCTCATCTATGCTAATCCCTGCAAAGCAAGCTCTCACATCAAGTACGCTGCTGAAGTTGGTGTTAACCTAACCACTTTCGACTCGGTAGATGAGGTTGAAAAGATCCGAAAATGGCACCCGAAATGTTCTTTACTGATCCGCATCAAAGCTCCGGACGAGAGCGGATCCCAGTGGCCACAGGGTTCCAAGTTCGGTGCTCTCCCTGACGAAGTGTCTCCGCTCCTCCAAGCCGCTCAAACTGCAGGACTCAAAGTTTCTGGGGTTTCATTTCACATAGGAAGCCGGTCACACAATTTTCAAGCATACAAAAAAGCTATAGAGGCAGCTAAGGTGGTATTCCAGGAGGCGTCCGGGCTCCGCATGCCTCCCATGCATGTGTTGAACATCGGCGGTGGCTTCCTGTCCGAGCCATCGTTCTTTGAAGCTGCGGCGGCAGCGGTGAAAACCGCTGTGGAGACGTATTTTTCAGAGGAGGAGCGACGCAGCTTAACGGTGATCGCCGAGCCGGGTCGGTTTTTTGCAGAGACTCCTTTCACGCTTGTTACCAATATCATCGGGAAGCGTGTGAGAGGGGAGCTCAGACAATACTGGATCAACGAAGGGTTTTTCGGGTCCATGATGCTTGCGGAACCTAAAACCCTACTTGAATCCTTCATTGCTCTGAAATGCATGTCCAATAACAGTGACGACGGCATCGAGAAGAATCCGGTTCCAGTCTTGGTGGTATGTGGCAAAGGTACTGATGGTGCTCGGACTTACAGTTCGACTGTGTTTGGTCCGACATGTGATTCCGATGACAAGGTGTTGGATGGTGAGTTACCAGAACTGGAAGTGAATAATTGGCTGGTGTTTCGCAACATGGGTGCTTATGCGTCGGCGTGCAGCACCAACTTCAATGGTTTTGGCTCCGCCAAGATCACCTATCTTACTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko00480 ko01100 ko01110 ko01130 map00330 map00480 map01100 map01110 map01130
Pfam Domains
Protein Families
Orn_Arg_deC_N Orn_DAP_Arg_deC

Protein Analysis

452

Amino Acids

49.5

Weight (kDa)

5.98

Isoelectric Point (pI)

48.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Orn_DAP_Arg_deC PF00278 77 - 428 9.9e-11 Pyridoxal-dependent decarboxylase, C-terminal sheet domain
Orn_Arg_deC_N PF02784 81 - 314 6.2e-66 Pyridoxal-dependent decarboxylase, pyridoxal binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 534
AccBSI CCGCTC 3 cut(s) 582, 640, 653
AccIII TCCGGA 1 cut(s) 571
AclWI GGATC 7 cut(s) 330, 423, 517, 550, 579, 592, 1019
AcoI YGGCCR 2 cut(s) 67, 593
AcsI RAATTY 1 cut(s) 355
AcuI CTGAAG 1 cut(s) 492
AcyI GRCGYC 1 cut(s) 767
AfaI GTAC 2 cut(s) 464, 1159
AfiI CCNNNNNNNGG 2 cut(s) 280, 319
AflIII ACRYGT 1 cut(s) 1202
AgsI TTSAA 7 cut(s) 354, 518, 719, 802, 842, 1067, 1315
AhdI GACNNNNNGTC 1 cut(s) 631
AjnI CCWGG 2 cut(s) 225, 759
AloI GAACNNNNNNTCC 2 cut(s) 1165, 1197
AluBI AGCT 9 cut(s) 8, 344, 450, 569, 734, 746, 845, 906, 997
AluI AGCT 9 cut(s) 8, 344, 450, 569, 734, 746, 845, 906, 997
Alw21I GWGCWC 4 cut(s) 118, 619, 999, 1171
Alw26I GTCTC 4 cut(s) 251, 639, 869, 938
Alw44I GTGCAC 1 cut(s) 114
AlwI GGATC 7 cut(s) 330, 423, 517, 550, 579, 592, 1019
Aor13HI TCCGGA 1 cut(s) 571
AoxI GGCC 2 cut(s) 67, 593
ApaLI GTGCAC 1 cut(s) 114
ApeKI GCWGC 7 cut(s) 199, 467, 743, 845, 854, 903, 1302
ApoI RAATTY 1 cut(s) 355
ArsI GACNNNNNNTTYG 2 cut(s) 1165, 1197
AspS9I GGNCC 3 cut(s) 283, 1032, 1196
AsuC2I CCSGG 3 cut(s) 320, 772, 927
AsuHPI GGTGA 7 cut(s) 71, 161, 308, 871, 925, 1244, 1330
AvaII GGWCC 3 cut(s) 283, 1032, 1196
BaeGI GKGCMC 1 cut(s) 118
BalI TGGCCA 1 cut(s) 595
BamHI GGATCC 1 cut(s) 584
BanI GGYRCC 1 cut(s) 534
BanII GRGCYC 2 cut(s) 777, 999
Bbv12I GWGCWC 4 cut(s) 118, 619, 999, 1171
BbvI GCAGC 7 cut(s) 211, 454, 755, 832, 866, 915, 1314
BccI CCATC 4 cut(s) 194, 838, 1157, 1223
BceAI ACGGC 1 cut(s) 1126
BciT130I CCWGG 2 cut(s) 227, 761
BcnI CCSGG 3 cut(s) 320, 772, 927
BcoDI GTCTC 4 cut(s) 251, 639, 869, 938
BfaI CTAG 1 cut(s) 401
BfmI CTRYAG 3 cut(s) 197, 660, 735
Bme1390I CCNGG 5 cut(s) 227, 320, 761, 772, 927
Bme18I GGWCC 3 cut(s) 283, 1032, 1196
BmeRI GACNNNNNGTC 1 cut(s) 631
BmgT120I GGNCC 3 cut(s) 283, 1032, 1196
BmiI GGNNCC 8 cut(s) 536, 586, 604, 776, 1033, 1050, 1131, 1327
BmrFI CCNGG 5 cut(s) 227, 320, 761, 772, 927
BmrI ACTGGG 1 cut(s) 583
BmsI GCATC 3 cut(s) 570, 1029, 1122
BmuI ACTGGG 1 cut(s) 583
BplI GAGNNNNNCTC 2 cut(s) 981, 1013
Bpu10I CCTNAGC 1 cut(s) 747
BpuEI CTTGAG 1 cut(s) 330
BpuMI CCSGG 3 cut(s) 320, 772, 927
BsaBI GATNNNNATC 1 cut(s) 560
BsaHI GRCGYC 1 cut(s) 767
BsaWI WCCGGW 2 cut(s) 571, 1126
Bsc4I CCNNNNNNNGG 2 cut(s) 280, 319
Bse118I RCCGGY 1 cut(s) 702
Bse1I ACTGG 5 cut(s) 39, 589, 1013, 1133, 1251
Bse3DI GCAATG 2 cut(s) 112, 1074
Bse8I GATNNNNATC 1 cut(s) 560
BseAI TCCGGA 1 cut(s) 571
BseBI CCWGG 2 cut(s) 227, 761
BseGI GGATG 1 cut(s) 1234
BseJI GATNNNNATC 1 cut(s) 560
BseLI CCNNNNNNNGG 2 cut(s) 280, 319
BseMI GCAATG 2 cut(s) 112, 1074
BseMII CTCAG 2 cut(s) 369, 1012
BseNI ACTGG 5 cut(s) 39, 589, 1013, 1133, 1251
BseRI GAGGAG 3 cut(s) 632, 905, 908
BseSI GKGCMC 1 cut(s) 118
BseXI GCAGC 7 cut(s) 211, 454, 755, 832, 866, 915, 1314
BsgI GTGCAG 1 cut(s) 1321
BshFI GGCC 2 cut(s) 69, 595
BshNI GGYRCC 1 cut(s) 534
BsiHKAI GWGCWC 4 cut(s) 118, 619, 999, 1171
BsiSI CCGG 8 cut(s) 70, 320, 413, 572, 703, 771, 926, 1127
BslI CCNNNNNNNGG 2 cut(s) 280, 319
BsmAI GTCTC 4 cut(s) 251, 639, 869, 938
BsmBI CGTCTC 1 cut(s) 869
BsnI GGCC 2 cut(s) 69, 595
Bsp1286I GDGCHC 5 cut(s) 118, 619, 777, 999, 1171
Bsp13I TCCGGA 1 cut(s) 571
BspANI GGCC 2 cut(s) 69, 595
BspCNI CTCAG 2 cut(s) 370, 1011
BspEI TCCGGA 1 cut(s) 571
BspLI GGNNCC 8 cut(s) 536, 586, 604, 776, 1033, 1050, 1131, 1327
BspMAI CTGCAG 2 cut(s) 201, 664
BspPI GGATC 7 cut(s) 330, 423, 517, 550, 579, 592, 1019
BspT107I GGYRCC 1 cut(s) 534
BsrBI CCGCTC 3 cut(s) 582, 640, 653
BsrDI GCAATG 2 cut(s) 112, 1074
BsrFI RCCGGY 1 cut(s) 702
BsrI ACTGG 5 cut(s) 39, 589, 1013, 1133, 1251
BssAI RCCGGY 1 cut(s) 702
BssNI GRCGYC 1 cut(s) 767
Bst2UI CCWGG 2 cut(s) 227, 761
Bst4CI ACNGT 6 cut(s) 143, 365, 913, 1103, 1181, 1189
BstACI GRCGYC 1 cut(s) 767
BstC8I GCNNGC 4 cut(s) 448, 782, 1044, 1300
BstDEI CTNAG 3 cut(s) 378, 747, 998
BstF5I GGATG 1 cut(s) 1234
BstMAI GTCTC 4 cut(s) 251, 639, 869, 938
BstMWI GCNNNNNNNGC 7 cut(s) 447, 659, 740, 781, 790, 851, 854
BstNI CCWGG 2 cut(s) 227, 761
BstNSI RCATGY 4 cut(s) 784, 797, 1093, 1206
BstSCI CCNGG 5 cut(s) 225, 318, 759, 770, 925
BstSFI CTRYAG 3 cut(s) 197, 660, 735
BstSLI GKGCMC 1 cut(s) 118
BstV1I GCAGC 7 cut(s) 211, 454, 755, 832, 866, 915, 1314
BstX2I RGATCY 3 cut(s) 322, 522, 584
BstYI RGATCY 3 cut(s) 322, 522, 584
BsuRI GGCC 2 cut(s) 69, 595
BtsCI GGATG 1 cut(s) 1234
BtsIMutI CAGTG 2 cut(s) 596, 1108
Cac8I GCNNGC 4 cut(s) 448, 782, 1044, 1300
Cfr10I RCCGGY 1 cut(s) 702
Cfr13I GGNCC 3 cut(s) 283, 1032, 1196
CseI GACGC 3 cut(s) 756, 909, 1281
Csp6I GTAC 2 cut(s) 463, 1158
CviQI GTAC 2 cut(s) 463, 1158
DdeI CTNAG 3 cut(s) 378, 747, 998
DriI GACNNNNNGTC 1 cut(s) 631
EaeI YGGCCR 2 cut(s) 67, 593
Eam1105I GACNNNNNGTC 1 cut(s) 631
EciI GGCGGA 1 cut(s) 1318
Ecl136II GAGCTC 1 cut(s) 997
Eco24I GRGCYC 2 cut(s) 777, 999
Eco47I GGWCC 3 cut(s) 283, 1032, 1196
Eco53kI GAGCTC 1 cut(s) 997
Eco57I CTGAAG 1 cut(s) 492
EcoICRI GAGCTC 1 cut(s) 997
EcoRII CCWGG 2 cut(s) 225, 759
EcoT22I ATGCAT 2 cut(s) 795, 1091
EcoT38I GRGCYC 2 cut(s) 777, 999
Esp3I CGTCTC 1 cut(s) 869
FokI GGATG 1 cut(s) 1241
FriOI GRGCYC 2 cut(s) 777, 999
FspBI CTAG 1 cut(s) 401
HaeIII GGCC 2 cut(s) 69, 595
HapII CCGG 8 cut(s) 70, 320, 413, 572, 703, 771, 926, 1127
HgaI GACGC 3 cut(s) 756, 909, 1281
Hin1I GRCGYC 1 cut(s) 767
HincII GTYRAC 1 cut(s) 484
HindII GTYRAC 1 cut(s) 484
HinfI GANTC 9 cut(s) 42, 73, 98, 500, 666, 946, 1067, 1123, 1208
HpaI GTTAAC 1 cut(s) 484
HpaII CCGG 8 cut(s) 70, 320, 413, 572, 703, 771, 926, 1127
HphI GGTGA 7 cut(s) 71, 161, 308, 871, 925, 1244, 1330
Hpy166II GTNNAC 2 cut(s) 116, 484
Hpy188I TCNGA 9 cut(s) 379, 527, 826, 889, 1001, 1083, 1173, 1200, 1213
Hpy188III TCNNGA 4 cut(s) 190, 572, 976, 1117
Hpy8I GTNNAC 2 cut(s) 116, 484
Hpy99I CGWCG 3 cut(s) 903, 1112, 1297
HpyAV CCTTC 3 cut(s) 284, 1013, 1081
HpyCH4III ACNGT 6 cut(s) 143, 365, 913, 1103, 1181, 1189
HpyCH4IV ACGT 4 cut(s) 222, 268, 335, 878
HpyF10VI GCNNNNNNNGC 7 cut(s) 447, 659, 740, 781, 790, 851, 854
HpyF3I CTNAG 3 cut(s) 378, 747, 998
HpySE526I ACGT 4 cut(s) 222, 268, 335, 878
Hsp92I GRCGYC 1 cut(s) 767
Kpn2I TCCGGA 1 cut(s) 571
KspAI GTTAAC 1 cut(s) 484
LmnI GCTCC 6 cut(s) 574, 645, 780, 895, 994, 1331
Lsp1109I GCAGC 7 cut(s) 211, 454, 755, 832, 866, 915, 1314
LweI GCATC 3 cut(s) 570, 1029, 1122
MaeI CTAG 1 cut(s) 401
MaeII ACGT 4 cut(s) 222, 268, 335, 878
MaeIII GTNAC 5 cut(s) 269, 705, 961, 1103, 1236
MbiI CCGCTC 3 cut(s) 582, 640, 653
MboII GAAGA 2 cut(s) 317, 1132
MflI RGATCY 3 cut(s) 322, 522, 584
MhlI GDGCHC 5 cut(s) 118, 619, 777, 999, 1171
MlsI TGGCCA 1 cut(s) 595
MluCI AATT 4 cut(s) 204, 355, 712, 1258
MluNI TGGCCA 1 cut(s) 595
MlyI GAGTC 6 cut(s) 51, 82, 107, 494, 660, 940
MmeI TCCRAC 3 cut(s) 310, 1206, 1223
Mox20I TGGCCA 1 cut(s) 595
Mph1103I ATGCAT 2 cut(s) 795, 1091
MroI TCCGGA 1 cut(s) 571
MscI TGGCCA 1 cut(s) 595
MseI TTAA 2 cut(s) 483, 908
MslI CAYNNNNRTG 1 cut(s) 794
Msp20I TGGCCA 1 cut(s) 595
MspA1I CMGCKG 2 cut(s) 857, 869
MspI CCGG 8 cut(s) 70, 320, 413, 572, 703, 771, 926, 1127
MspR9I CCNGG 5 cut(s) 227, 320, 761, 772, 927
MvaI CCWGG 2 cut(s) 227, 761
MwoI GCNNNNNNNGC 7 cut(s) 447, 659, 740, 781, 790, 851, 854
NciI CCSGG 3 cut(s) 320, 772, 927
NlaIV GGNNCC 8 cut(s) 536, 586, 604, 776, 1033, 1050, 1131, 1327
NmeAIII GCCGAG 1 cut(s) 946
NmuCI GTSAC 2 cut(s) 705, 1103
NsiI ATGCAT 2 cut(s) 795, 1091
NspI RCATGY 4 cut(s) 784, 797, 1093, 1206
PaeI GCATGC 1 cut(s) 784
PciI ACATGT 1 cut(s) 1202
PcsI WCGNNNNNNNCGW 1 cut(s) 1113
PfeI GAWTC 3 cut(s) 1067, 1123, 1208
PfoI TCCNGGA 2 cut(s) 225, 759
PleI GAGTC 6 cut(s) 50, 81, 106, 494, 660, 940
PpsI GAGTC 6 cut(s) 50, 81, 106, 494, 660, 940
PscI ACATGT 1 cut(s) 1202
Psp124BI GAGCTC 1 cut(s) 999
Psp6I CCWGG 2 cut(s) 225, 759
PspGI CCWGG 2 cut(s) 225, 759
PspN4I GGNNCC 8 cut(s) 536, 586, 604, 776, 1033, 1050, 1131, 1327
PspPI GGNCC 3 cut(s) 283, 1032, 1196
PstI CTGCAG 2 cut(s) 201, 664
PsuI RGATCY 3 cut(s) 322, 522, 584
RsaI GTAC 2 cut(s) 464, 1159
RsaNI GTAC 2 cut(s) 463, 1158
RseI CAYNNNNRTG 1 cut(s) 794
SacI GAGCTC 1 cut(s) 999
SaqAI TTAA 2 cut(s) 483, 908
Sau96I GGNCC 3 cut(s) 283, 1032, 1196
SchI GAGTC 6 cut(s) 51, 82, 107, 494, 660, 940
ScrFI CCNGG 5 cut(s) 227, 320, 761, 772, 927
SduI GDGCHC 5 cut(s) 118, 619, 777, 999, 1171
SfaNI GCATC 3 cut(s) 570, 1029, 1122
SfcI CTRYAG 3 cut(s) 197, 660, 735
SinI GGWCC 3 cut(s) 283, 1032, 1196
SmiMI CAYNNNNRTG 1 cut(s) 794
SmlI CTYRAG 1 cut(s) 345
SmoI CTYRAG 1 cut(s) 345
SphI GCATGC 1 cut(s) 784
Sse9I AATT 4 cut(s) 204, 355, 712, 1258
SspMI CTAG 1 cut(s) 401
SstI GAGCTC 1 cut(s) 999
StyD4I CCNGG 5 cut(s) 225, 318, 759, 770, 925
TaaI ACNGT 6 cut(s) 143, 365, 913, 1103, 1181, 1189
TaiI ACGT 4 cut(s) 225, 271, 338, 881
TaqI TCGA 4 cut(s) 360, 498, 1116, 1184
TasI AATT 4 cut(s) 204, 355, 712, 1258
TauI GCSGC 3 cut(s) 653, 851, 854
TfiI GAWTC 3 cut(s) 1067, 1123, 1208
Tru1I TTAA 2 cut(s) 483, 908
Tru9I TTAA 2 cut(s) 483, 908
TscAI CASTG 2 cut(s) 596, 1108
TseFI GTSAC 2 cut(s) 705, 1103
TseI GCWGC 7 cut(s) 199, 467, 743, 845, 854, 903, 1302
Tsp45I GTSAC 2 cut(s) 705, 1103
TspDTI ATGAA 5 cut(s) 51, 154, 171, 675, 1063
TspGWI ACGGA 2 cut(s) 289, 363
TspRI CASTG 2 cut(s) 596, 1108
VneI GTGCAC 1 cut(s) 114
VpaK11BI GGWCC 3 cut(s) 283, 1032, 1196
XapI RAATTY 1 cut(s) 355
XceI RCATGY 4 cut(s) 784, 797, 1093, 1206
XspI CTAG 1 cut(s) 401
Zsp2I ATGCAT 2 cut(s) 795, 1091
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.