MD05G1034000.v1.1

Belongs to the Orn Lys Arg decarboxylase class-II family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000734
Physical Location & Seq
Forward (+)
5447978 .. 5449255
1278 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1034000.v1.1.491

Sequence Viewer

Length: 1278 bp
ATGGCATTCATCCTTCCAAGTATTCCAAGCCTTACTACTTTTAGCAGCCTCCAAGTCAAATTGGCAGCAAGCCAAGTGAAGGGTGAGCATGTTCCAACTGACATACCAAGACACCAACTCACCACTCTCATCAGATCCATCATCAACAAACAAGACGAACCACAACCCTTTTACGTTCTCGATTTGGGAGTTCTTGTCAGCCTTATGCAAAAATGGAACCGTTGCCTTCCCAACGTTCAGCCCTTCTTTGCTGTCAAGTGTAACCCTGAGCCTGCATTCATTGCAGCATTGGCAACGCTCGGTGCGAGCTTTGACTGCGCTAGCAAGGTGGAGATCGAGGCCATCCTTTCTCATGGTGTAAGCCCCTCAAGGATAGTCTACGCCAATCCATGCAAGGGTGAGAACCATTTAAGGCACGCAGCTGGAGTCGGGGTCAATCTTGCAACCTTCGACTCCATGGACGAACTTAACAAGATAAAAAGATGCCATCCCAAATGTTCCTTGTTGCTCCGAATTGCAGTCCCAAATGACACGAGCTCCTGGCGTTCATTTGGGACCAAATTCGGTGCGTTGTCCGAGGAGGTGGCGCCTCTCCTTAGACATGCACACAAACTAGGGTTGAGAGTGGTGGGAGTTTCATTTCACGTGGGTTCGAAAGCTTCGGAATCTCAAGTGTACCGTGGAGCAATAGCAGCTGCTAAGGCTGCATTCGACGTGGCAGATGAGCTCCAACTGCCCAAAATGCATGTGCTTGATATTGGTGGTGGGTTCAAGGCAAACCCGTTATTTGATGAAATAGCCGAAACCCTAAACGACGCTATTAAAGAACACTTTGGTGATCATCAATCGGAGTCTGATCTGGTGGTGATGGCTGAGCCAGGAAGGTTCTTTGCCGAAATGGCTTTCACAATGGTTGCAAATGTGATGGGGAAGCGAGTGAGAGGAGAGAAAAGAGAGTATTGGATTACCGATGGGATATATGGGTCGTTTAATCTTCCGGCATTTGATAAATCTTGTTTGACAGTTTCACCCCTCCTCCTTCACACTCCTGATCATGATCATCATTCAGTAGCCATATATTCGTCGACGGTGTTCGGACCAACCTGTGATTCGCTGGACACGGTGGTGGCTGACTGCAAACTGCCGGAACTGAAGTTAAATGATTATCTCGTGTTTCATAACATGGGGGCTTACACAAATTCGGTTGGAACCAACTTTAATGGCTTCTGCATTTCTGCTATTCCCACGTACTTAACATTTACAAGTGCTAATAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko00480 ko01100 ko01110 ko01130 map00330 map00480 map01100 map01110 map01130
Pfam Domains
Protein Families
Orn_Arg_deC_N Orn_DAP_Arg_deC

Protein Analysis

426

Amino Acids

46.45

Weight (kDa)

6.37

Isoelectric Point (pI)

42.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Orn_DAP_Arg_deC PF00278 58 - 396 9.4e-17 Pyridoxal-dependent decarboxylase, C-terminal sheet domain
Orn_Arg_deC_N PF02784 65 - 298 3.5e-65 Pyridoxal-dependent decarboxylase, pyridoxal binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 586
AccI GTMKAC 2 cut(s) 378, 1085
AclI AACGTT 1 cut(s) 234
AclWI GGATC 1 cut(s) 129
AcsI RAATTY 2 cut(s) 560, 1198
AcuI CTGAAG 1 cut(s) 1172
AcvI CACGTG 1 cut(s) 646
AcyI GRCGYC 1 cut(s) 587
AfaI GTAC 2 cut(s) 677, 1250
AfiI CCNNNNNNNGG 2 cut(s) 79, 395
AgsI TTSAA 1 cut(s) 772
AjiI CACGTC 1 cut(s) 715
AjnI CCWGG 2 cut(s) 539, 877
AjuI GAANNNNNNNTTGG 2 cut(s) 1093, 1125
AleI CACNNNNGTG 2 cut(s) 834, 1124
AluBI AGCT 6 cut(s) 309, 422, 537, 659, 695, 727
AluI AGCT 6 cut(s) 309, 422, 537, 659, 695, 727
Alw21I GWGCWC 2 cut(s) 539, 729
AlwI GGATC 1 cut(s) 129
AoxI GGCC 1 cut(s) 339
ApeKI GCWGC 7 cut(s) 45, 65, 284, 419, 692, 695, 704
ApoI RAATTY 2 cut(s) 560, 1198
AspLEI GCGC 2 cut(s) 320, 589
AspS9I GGNCC 2 cut(s) 555, 1097
AsuHPI GGTGA 6 cut(s) 95, 112, 410, 848, 877, 1020
AsuII TTCGAA 1 cut(s) 653
AsuNHI GCTAGC 1 cut(s) 320
AvaII GGWCC 2 cut(s) 555, 1097
BanI GGYRCC 1 cut(s) 586
BanII GRGCYC 2 cut(s) 539, 729
BauI CACGAG 2 cut(s) 532, 1169
BbrPI CACGTG 1 cut(s) 646
Bbv12I GWGCWC 2 cut(s) 539, 729
BbvI GCAGC 7 cut(s) 57, 77, 296, 431, 682, 691, 704
BccI CCATC 6 cut(s) 146, 350, 495, 862, 919, 965
BciT130I CCWGG 2 cut(s) 541, 879
BclI TGATCA 3 cut(s) 838, 1051, 1057
BfaI CTAG 2 cut(s) 321, 614
BfoI RGCGCY 1 cut(s) 590
BglI GCCNNNNNGGC 1 cut(s) 899
BisI GCNGC 7 cut(s) 46, 66, 285, 420, 693, 696, 705
BlpI GCTNAGC 1 cut(s) 873
BlsI GCNGC 7 cut(s) 47, 67, 286, 421, 694, 697, 706
Bme1390I CCNGG 2 cut(s) 541, 879
Bme18I GGWCC 2 cut(s) 555, 1097
BmgBI CACGTC 1 cut(s) 715
BmgT120I GGNCC 2 cut(s) 555, 1097
BmiI GGNNCC 4 cut(s) 218, 556, 588, 1210
BmrFI CCNGG 2 cut(s) 541, 879
BmsI GCATC 1 cut(s) 473
BmtI GCTAGC 1 cut(s) 324
BpmI CTGGAG 1 cut(s) 444
Bpu10I CCTNAGC 2 cut(s) 267, 699
Bpu1102I GCTNAGC 1 cut(s) 873
Bpu14I TTCGAA 1 cut(s) 653
BpuEI CTTGAG 2 cut(s) 352, 654
BsaAI YACGTR 2 cut(s) 646, 1248
BsaBI GATNNNNATC 1 cut(s) 1056
BsaHI GRCGYC 1 cut(s) 587
BsaJI CCNNGG 3 cut(s) 456, 576, 679
BsaXI ACNNNNNCTCC 8 cut(s) 180, 210, 417, 447, 521, 551, 1020, 1050
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 395
Bse3DI GCAATG 1 cut(s) 279
Bse8I GATNNNNATC 1 cut(s) 1056
BseBI CCWGG 2 cut(s) 541, 879
BseDI CCNNGG 3 cut(s) 456, 576, 679
BseGI GGATG 3 cut(s) 9, 342, 487
BseJI GATNNNNATC 1 cut(s) 1056
BseLI CCNNNNNNNGG 2 cut(s) 79, 395
BseMI GCAATG 1 cut(s) 279
BseMII CTCAG 2 cut(s) 258, 864
BseRI GAGGAG 3 cut(s) 593, 957, 1025
BseXI GCAGC 7 cut(s) 57, 77, 296, 431, 682, 691, 704
BshFI GGCC 1 cut(s) 341
BshNI GGYRCC 1 cut(s) 586
BsiHKAI GWGCWC 2 cut(s) 539, 729
BsiSI CCGG 2 cut(s) 998, 1145
BslFI GGGAC 2 cut(s) 506, 568
BslI CCNNNNNNNGG 2 cut(s) 79, 395
BsmFI GGGAC 2 cut(s) 506, 568
BsmI GAATGC 3 cut(s) 5, 275, 707
BsnI GGCC 1 cut(s) 341
Bsp119I TTCGAA 1 cut(s) 653
Bsp1286I GDGCHC 2 cut(s) 539, 729
Bsp143I GATC 6 cut(s) 134, 333, 838, 856, 1051, 1057
Bsp1720I GCTNAGC 1 cut(s) 873
Bsp19I CCATGG 1 cut(s) 456
BspANI GGCC 1 cut(s) 341
BspCNI CTCAG 2 cut(s) 259, 865
BspHI TCATGA 1 cut(s) 1054
BspLI GGNNCC 4 cut(s) 218, 556, 588, 1210
BspOI GCTAGC 1 cut(s) 324
BspPI GGATC 1 cut(s) 129
BspT104I TTCGAA 1 cut(s) 653
BspT107I GGYRCC 1 cut(s) 586
BsrDI GCAATG 1 cut(s) 279
BssECI CCNNGG 3 cut(s) 456, 576, 679
BssMI GATC 6 cut(s) 134, 333, 838, 856, 1051, 1057
BssNI GRCGYC 1 cut(s) 587
BssSI CACGAG 2 cut(s) 532, 1169
BssT1I CCWWGG 1 cut(s) 456
Bst2BI CACGAG 2 cut(s) 532, 1169
Bst2UI CCWGG 2 cut(s) 541, 879
Bst4CI ACNGT 5 cut(s) 221, 680, 1024, 1090, 1123
BstACI GRCGYC 1 cut(s) 587
BstAPI GCANNNNNTGC 1 cut(s) 281
BstBAI YACGTR 2 cut(s) 646, 1248
BstBI TTCGAA 1 cut(s) 653
BstC8I GCNNGC 5 cut(s) 70, 273, 307, 322, 417
BstDEI CTNAG 4 cut(s) 267, 596, 699, 873
BstDSI CCRYGG 2 cut(s) 456, 679
BstF5I GGATG 3 cut(s) 9, 342, 487
BstH2I RGCGCY 1 cut(s) 590
BstHHI GCGC 2 cut(s) 320, 589
BstKTI GATC 6 cut(s) 137, 336, 841, 859, 1054, 1060
BstMBI GATC 6 cut(s) 134, 333, 838, 856, 1051, 1057
BstMWI GCNNNNNNNGC 9 cut(s) 281, 290, 315, 692, 701, 704, 733, 742, 899
BstNI CCWGG 2 cut(s) 541, 879
BstNSI RCATGY 3 cut(s) 92, 605, 749
BstSCI CCNGG 2 cut(s) 539, 877
BstV1I GCAGC 7 cut(s) 57, 77, 296, 431, 682, 691, 704
BstX2I RGATCY 1 cut(s) 134
BstYI RGATCY 1 cut(s) 134
BsuRI GGCC 1 cut(s) 341
BtgI CCRYGG 2 cut(s) 456, 679
BtrI CACGTC 1 cut(s) 715
BtsCI GGATG 3 cut(s) 9, 342, 487
Cac8I GCNNGC 5 cut(s) 70, 273, 307, 322, 417
CciI TCATGA 1 cut(s) 1054
CfoI GCGC 2 cut(s) 320, 589
Cfr13I GGNCC 2 cut(s) 555, 1097
CseI GACGC 1 cut(s) 824
Csp6I GTAC 2 cut(s) 676, 1249
CviAII CATG 8 cut(s) 89, 353, 390, 457, 602, 746, 1055, 1183
CviQI GTAC 2 cut(s) 676, 1249
DdeI CTNAG 4 cut(s) 267, 596, 699, 873
DinI GGCGCC 1 cut(s) 588
DpnI GATC 6 cut(s) 136, 335, 840, 858, 1053, 1059
DpnII GATC 6 cut(s) 134, 333, 838, 856, 1051, 1057
Ecl136II GAGCTC 2 cut(s) 537, 727
Eco130I CCWWGG 1 cut(s) 456
Eco24I GRGCYC 2 cut(s) 539, 729
Eco47I GGWCC 2 cut(s) 555, 1097
Eco53kI GAGCTC 2 cut(s) 537, 727
Eco57I CTGAAG 1 cut(s) 1172
Eco72I CACGTG 1 cut(s) 646
EcoICRI GAGCTC 2 cut(s) 537, 727
EcoRII CCWGG 2 cut(s) 539, 877
EcoT14I CCWWGG 1 cut(s) 456
EcoT22I ATGCAT 1 cut(s) 747
EcoT38I GRGCYC 2 cut(s) 539, 729
EgeI GGCGCC 1 cut(s) 588
EheI GGCGCC 1 cut(s) 588
ErhI CCWWGG 1 cut(s) 456
FaeI CATG 8 cut(s) 92, 356, 393, 460, 605, 749, 1058, 1186
FaqI GGGAC 2 cut(s) 506, 568
FatI CATG 8 cut(s) 88, 352, 389, 456, 601, 745, 1054, 1182
FbaI TGATCA 3 cut(s) 838, 1051, 1057
FblI GTMKAC 2 cut(s) 378, 1085
Fnu4HI GCNGC 7 cut(s) 46, 66, 285, 420, 693, 696, 705
FokI GGATG 2 cut(s) 329, 474
FriOI GRGCYC 2 cut(s) 539, 729
Fsp4HI GCNGC 7 cut(s) 46, 66, 285, 420, 693, 696, 705
FspBI CTAG 2 cut(s) 321, 614
GlaI GCGC 2 cut(s) 319, 588
GluI GCNGC 7 cut(s) 46, 66, 285, 420, 693, 696, 705
GsuI CTGGAG 1 cut(s) 444
HaeII RGCGCY 1 cut(s) 590
HaeIII GGCC 1 cut(s) 341
HapII CCGG 2 cut(s) 998, 1145
HgaI GACGC 1 cut(s) 824
HhaI GCGC 2 cut(s) 320, 589
Hin1I GRCGYC 1 cut(s) 587
Hin1II CATG 8 cut(s) 92, 356, 393, 460, 605, 749, 1058, 1186
Hin6I GCGC 2 cut(s) 318, 587
HinP1I GCGC 2 cut(s) 318, 587
HincII GTYRAC 1 cut(s) 1086
HindII GTYRAC 1 cut(s) 1086
HindIII AAGCTT 1 cut(s) 657
HinfI GANTC 5 cut(s) 426, 452, 665, 851, 1109
HpaII CCGG 2 cut(s) 998, 1145
HphI GGTGA 6 cut(s) 95, 112, 410, 848, 877, 1020
Hpy166II GTNNAC 3 cut(s) 379, 676, 1086
Hpy188I TCNGA 7 cut(s) 134, 512, 577, 664, 850, 856, 1097
Hpy188III TCNNGA 3 cut(s) 179, 1049, 1055
Hpy8I GTNNAC 3 cut(s) 379, 676, 1086
Hpy99I CGWCG 4 cut(s) 716, 818, 1087, 1090
HpyAV CCTTC 7 cut(s) 23, 73, 236, 253, 457, 876, 1049
HpyCH4III ACNGT 5 cut(s) 221, 680, 1024, 1090, 1123
HpyCH4IV ACGT 5 cut(s) 174, 234, 645, 714, 1247
HpyF10VI GCNNNNNNNGC 9 cut(s) 281, 290, 315, 692, 701, 704, 733, 742, 899
HpyF3I CTNAG 4 cut(s) 267, 596, 699, 873
HpySE526I ACGT 5 cut(s) 174, 234, 645, 714, 1247
Hsp92I GRCGYC 1 cut(s) 587
Hsp92II CATG 8 cut(s) 92, 356, 393, 460, 605, 749, 1058, 1186
HspAI GCGC 2 cut(s) 318, 587
KasI GGCGCC 1 cut(s) 586
Ksp22I TGATCA 3 cut(s) 838, 1051, 1057
Kzo9I GATC 6 cut(s) 134, 333, 838, 856, 1051, 1057
LmnI GCTCC 4 cut(s) 513, 542, 683, 732
Lsp1109I GCAGC 7 cut(s) 57, 77, 296, 431, 682, 691, 704
LweI GCATC 1 cut(s) 473
MaeI CTAG 2 cut(s) 321, 614
MaeII ACGT 5 cut(s) 174, 234, 645, 714, 1247
MaeIII GTNAC 1 cut(s) 260
MalI GATC 6 cut(s) 136, 335, 840, 858, 1053, 1059
MboI GATC 6 cut(s) 134, 333, 838, 856, 1051, 1057
MboII GAAGA 1 cut(s) 986
MflI RGATCY 1 cut(s) 134
MhlI GDGCHC 2 cut(s) 539, 729
MluCI AATT 5 cut(s) 59, 513, 560, 1198, 1273
Mly113I GGCGCC 1 cut(s) 587
MlyI GAGTC 3 cut(s) 435, 446, 860
MmeI TCCRAC 3 cut(s) 119, 754, 1186
MnlI CCTC 9 cut(s) 59, 331, 376, 571, 574, 600, 935, 1043, 1046
Mph1103I ATGCAT 1 cut(s) 747
MseI TTAA 8 cut(s) 410, 468, 822, 990, 1157, 1218, 1253, 1276
MslI CAYNNNNRTG 2 cut(s) 834, 1124
MspA1I CMGCKG 2 cut(s) 422, 695
MspI CCGG 2 cut(s) 998, 1145
MspR9I CCNGG 2 cut(s) 541, 879
Mva1269I GAATGC 3 cut(s) 5, 275, 707
MvaI CCWGG 2 cut(s) 541, 879
MwoI GCNNNNNNNGC 9 cut(s) 281, 290, 315, 692, 701, 704, 733, 742, 899
NarI GGCGCC 1 cut(s) 587
NcoI CCATGG 1 cut(s) 456
NdeII GATC 6 cut(s) 134, 333, 838, 856, 1051, 1057
NheI GCTAGC 1 cut(s) 320
NlaIII CATG 8 cut(s) 92, 356, 393, 460, 605, 749, 1058, 1186
NlaIV GGNNCC 4 cut(s) 218, 556, 588, 1210
NsiI ATGCAT 1 cut(s) 747
NspI RCATGY 3 cut(s) 92, 605, 749
NspV TTCGAA 1 cut(s) 653
OliI CACNNNNGTG 2 cut(s) 834, 1124
PagI TCATGA 1 cut(s) 1054
PcsI WCGNNNNNNNCGW 1 cut(s) 302
PctI GAATGC 3 cut(s) 5, 275, 707
PfeI GAWTC 2 cut(s) 665, 1109
PkrI GCNGC 7 cut(s) 47, 67, 286, 421, 694, 697, 706
PleI GAGTC 3 cut(s) 434, 446, 859
PluTI GGCGCC 1 cut(s) 590
PmaCI CACGTG 1 cut(s) 646
PmlI CACGTG 1 cut(s) 646
PpsI GAGTC 3 cut(s) 434, 446, 859
Ppu21I YACGTR 2 cut(s) 646, 1248
Psp124BI GAGCTC 2 cut(s) 539, 729
Psp1406I AACGTT 1 cut(s) 234
Psp6I CCWGG 2 cut(s) 539, 877
PspCI CACGTG 1 cut(s) 646
PspGI CCWGG 2 cut(s) 539, 877
PspN4I GGNNCC 4 cut(s) 218, 556, 588, 1210
PspPI GGNCC 2 cut(s) 555, 1097
PsuI RGATCY 1 cut(s) 134
PvuII CAGCTG 2 cut(s) 422, 695
RsaI GTAC 2 cut(s) 677, 1250
RsaNI GTAC 2 cut(s) 676, 1249
RseI CAYNNNNRTG 2 cut(s) 834, 1124
SacI GAGCTC 2 cut(s) 539, 729
SalI GTCGAC 1 cut(s) 1084
SaqAI TTAA 8 cut(s) 410, 468, 822, 990, 1157, 1218, 1253, 1276
SatI GCNGC 7 cut(s) 46, 66, 285, 420, 693, 696, 705
Sau3AI GATC 6 cut(s) 134, 333, 838, 856, 1051, 1057
Sau96I GGNCC 2 cut(s) 555, 1097
SchI GAGTC 3 cut(s) 435, 446, 860
ScrFI CCNGG 2 cut(s) 541, 879
SduI GDGCHC 2 cut(s) 539, 729
SfaNI GCATC 1 cut(s) 473
SfoI GGCGCC 1 cut(s) 588
SfuI TTCGAA 1 cut(s) 653
SgrDI CGTCGACG 1 cut(s) 1084
SinI GGWCC 2 cut(s) 555, 1097
SmiMI CAYNNNNRTG 2 cut(s) 834, 1124
SmlI CTYRAG 2 cut(s) 367, 669
SmoI CTYRAG 2 cut(s) 367, 669
Sse9I AATT 5 cut(s) 59, 513, 560, 1198, 1273
SspDI GGCGCC 1 cut(s) 586
SspMI CTAG 2 cut(s) 321, 614
SstI GAGCTC 2 cut(s) 539, 729
StyD4I CCNGG 2 cut(s) 539, 877
StyI CCWWGG 1 cut(s) 456
TaaI ACNGT 5 cut(s) 221, 680, 1024, 1090, 1123
TaiI ACGT 5 cut(s) 177, 237, 648, 717, 1250
TaqI TCGA 6 cut(s) 180, 336, 450, 653, 711, 1085
TasI AATT 5 cut(s) 59, 513, 560, 1198, 1273
TfiI GAWTC 2 cut(s) 665, 1109
Tru1I TTAA 8 cut(s) 410, 468, 822, 990, 1157, 1218, 1253, 1276
Tru9I TTAA 8 cut(s) 410, 468, 822, 990, 1157, 1218, 1253, 1276
TseI GCWGC 7 cut(s) 45, 65, 284, 419, 692, 695, 704
TspDTI ATGAA 5 cut(s) 268, 537, 627, 807, 1166
VpaK11BI GGWCC 2 cut(s) 555, 1097
XapI RAATTY 2 cut(s) 560, 1198
XceI RCATGY 3 cut(s) 92, 605, 749
XmiI GTMKAC 2 cut(s) 378, 1085
XspI CTAG 2 cut(s) 321, 614
Zsp2I ATGCAT 1 cut(s) 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.