MD05G1036600.v1.1

Belongs to the Orn Lys Arg decarboxylase class-II family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000734
Physical Location & Seq
Reverse (-)
5889578 .. 5890842
1265 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1036600.v1.1.491

Sequence Viewer

Length: 1143 bp
ATGGCATTGATCCTTCCAAGTATTCCAAGCATTACTGCTTTTAGCAGCCTCCAAGTCAAAATGGCAGCAAGCCAAGTGAAGGGTGAACATGTTCCAACTGACATACCAAGACACCAACTCACCGCTCACATCAGATCCATCATCAACAAACAAGACGAACCACAACCCTTTTATGTTCTCGATTTGGGAGTTCTTGTCAGCCTTATGCAAAAATGGAACCGTTGCCTTCCCAACGTTCTGCCCTTCTTTGCTGTCAAGTGTAACCCTGAGCCTGCATTCATAGCAGCATTGGCAACGCTCGGTGCGAGCTTTGACTGCGCTAGCAAGGTGGAGATCGAGGCCATCCTTTCTCATGGTGTAAGTCCCTCAAGGATAGTCTACGCCAATCCATGCAAGGTCCTCAATGACACGAGCTCCTGGCGTTCATTTGGGACCAAATTCGGTGCGTTGTCCGAGGAGGTCGCGCCTCTACTTAGACATGTAGACAAACTAGGGTTGAGAGTGGTGGGAGTTTCATTTCACGTGGGTTCGAAAGCTTCGGAATCTCAAGTGTACCGTGGAGCAATAGTAGCTGCTAGGGCTGCATTCGACGTGGCAGATGAGCTCAAACTGCCCAAAATGCATGTGCTTGATATTGGTGGTGGGTTCAAGGCAAACCCGTTATTTGATGAAATAGCCGAAACCCTAAACGACGCCATTAAAGAACACTTTGGTGATCATCAATCGGAGTCTGATCTGGTGGTGATGGCTGAGCCAGGAAGGTTCTTTGCGGAAACGCCTTTCACTTTGGTTGCAAATGTGATGGGGAAGAGAGTGAGAGGAGAGAAAAGAGAGTATTGGATTACTGATGGGATATATGGGTCGTTTAATCTTCCAGCATTTGATAAATCTTGTTTGACAGTTTCACCCCTCCTCCTTCACACTCCTGATCATCATCATCATTCAGTAGCCATATATTCATCGACGGTGTTCGGACCAACCTGTGATTCGCTGGACACGGTGGTGGCTGACTACAAACTGCCGGAACTGAAGTTAAATGATTATCTCGTGTTTCATAACATGGGGGCTTACACAAGTTCTGCTGGAACCAACTTTAATGGCTTCTGCATTTCTGCTATTCCCACGTACTTAACATTTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko00480 ko01100 ko01110 ko01130 map00330 map00480 map01100 map01110 map01130
Pfam Domains
Protein Families
Orn_Arg_deC_N Orn_DAP_Arg_deC

Protein Analysis

381

Amino Acids

41.57

Weight (kDa)

6.19

Isoelectric Point (pI)

42.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Orn_DAP_Arg_deC PF00278 58 - 355 1.9e-16 Pyridoxal-dependent decarboxylase, C-terminal sheet domain
Orn_Arg_deC_N PF02784 65 - 134 1.5e-21 Pyridoxal-dependent decarboxylase, pyridoxal binding domain
Orn_Arg_deC_N PF02784 144 - 257 1.3e-22 Pyridoxal-dependent decarboxylase, pyridoxal binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 125
AccI GTMKAC 2 cut(s) 378, 483
AccII CGCG 1 cut(s) 464
AciI CCGC 2 cut(s) 123, 770
AclI AACGTT 1 cut(s) 234
AclWI GGATC 2 cut(s) 4, 129
AcsI RAATTY 1 cut(s) 437
AcuI CTGAAG 1 cut(s) 1049
AcvI CACGTG 1 cut(s) 523
AcyI GRCGYC 1 cut(s) 693
AfaI GTAC 2 cut(s) 554, 1127
AfiI CCNNNNNNNGG 1 cut(s) 79
AflIII ACRYGT 2 cut(s) 88, 478
AgsI TTSAA 1 cut(s) 649
AjiI CACGTC 1 cut(s) 592
AjnI CCWGG 2 cut(s) 416, 754
AjuI GAANNNNNNNTTGG 2 cut(s) 970, 1002
AleI CACNNNNGTG 2 cut(s) 711, 1001
AluBI AGCT 5 cut(s) 309, 414, 536, 572, 604
AluI AGCT 5 cut(s) 309, 414, 536, 572, 604
Alw21I GWGCWC 2 cut(s) 416, 606
AlwI GGATC 2 cut(s) 4, 129
AoxI GGCC 1 cut(s) 339
ApeKI GCWGC 5 cut(s) 45, 65, 284, 572, 581
ApoI RAATTY 1 cut(s) 437
Asp700I GAANNNNTTC 1 cut(s) 90
AspLEI GCGC 2 cut(s) 320, 466
AspS9I GGNCC 3 cut(s) 397, 432, 974
AsuHPI GGTGA 5 cut(s) 95, 112, 725, 754, 897
AsuII TTCGAA 1 cut(s) 530
AsuNHI GCTAGC 1 cut(s) 320
AvaII GGWCC 3 cut(s) 397, 432, 974
BanII GRGCYC 2 cut(s) 416, 606
BauI CACGAG 2 cut(s) 409, 1046
BbrPI CACGTG 1 cut(s) 523
Bbv12I GWGCWC 2 cut(s) 416, 606
BbvI GCAGC 5 cut(s) 57, 77, 296, 559, 568
BccI CCATC 5 cut(s) 146, 350, 739, 796, 842
BciT130I CCWGG 2 cut(s) 418, 756
BclI TGATCA 2 cut(s) 715, 928
BfaI CTAG 3 cut(s) 321, 491, 576
BisI GCNGC 5 cut(s) 46, 66, 285, 573, 582
BlpI GCTNAGC 1 cut(s) 750
BlsI GCNGC 5 cut(s) 47, 67, 286, 574, 583
Bme1390I CCNGG 2 cut(s) 418, 756
Bme18I GGWCC 3 cut(s) 397, 432, 974
BmgBI CACGTC 1 cut(s) 592
BmgT120I GGNCC 3 cut(s) 397, 432, 974
BmiI GGNNCC 3 cut(s) 218, 433, 1087
BmrFI CCNGG 2 cut(s) 418, 756
BmtI GCTAGC 1 cut(s) 324
Bpu10I CCTNAGC 1 cut(s) 267
Bpu1102I GCTNAGC 1 cut(s) 750
Bpu14I TTCGAA 1 cut(s) 530
BpuEI CTTGAG 2 cut(s) 352, 531
BsaAI YACGTR 2 cut(s) 523, 1125
BsaBI GATNNNNATC 1 cut(s) 933
BsaHI GRCGYC 1 cut(s) 693
BsaJI CCNNGG 2 cut(s) 453, 556
BsaXI ACNNNNNCTCC 8 cut(s) 180, 210, 398, 428, 552, 582, 897, 927
Bsc4I CCNNNNNNNGG 1 cut(s) 79
Bse8I GATNNNNATC 1 cut(s) 933
BseBI CCWGG 2 cut(s) 418, 756
BseDI CCNNGG 2 cut(s) 453, 556
BseGI GGATG 1 cut(s) 342
BseJI GATNNNNATC 1 cut(s) 933
BseLI CCNNNNNNNGG 1 cut(s) 79
BseMII CTCAG 2 cut(s) 258, 741
BseRI GAGGAG 3 cut(s) 470, 834, 902
BseXI GCAGC 5 cut(s) 57, 77, 296, 559, 568
Bsh1236I CGCG 1 cut(s) 464
BshFI GGCC 1 cut(s) 341
BsiHKAI GWGCWC 2 cut(s) 416, 606
BsiSI CCGG 1 cut(s) 1022
BslFI GGGAC 2 cut(s) 348, 445
BslI CCNNNNNNNGG 1 cut(s) 79
BsmFI GGGAC 2 cut(s) 348, 445
BsmI GAATGC 2 cut(s) 275, 584
BsnI GGCC 1 cut(s) 341
Bsp119I TTCGAA 1 cut(s) 530
Bsp1286I GDGCHC 2 cut(s) 416, 606
Bsp143I GATC 6 cut(s) 9, 134, 333, 715, 733, 928
Bsp1720I GCTNAGC 1 cut(s) 750
BspACI CCGC 2 cut(s) 123, 770
BspANI GGCC 1 cut(s) 341
BspCNI CTCAG 2 cut(s) 259, 742
BspFNI CGCG 1 cut(s) 464
BspLI GGNNCC 3 cut(s) 218, 433, 1087
BspOI GCTAGC 1 cut(s) 324
BspPI GGATC 2 cut(s) 4, 129
BspT104I TTCGAA 1 cut(s) 530
BsrBI CCGCTC 1 cut(s) 125
BssECI CCNNGG 2 cut(s) 453, 556
BssMI GATC 6 cut(s) 9, 134, 333, 715, 733, 928
BssNI GRCGYC 1 cut(s) 693
BssSI CACGAG 2 cut(s) 409, 1046
Bst2BI CACGAG 2 cut(s) 409, 1046
Bst2UI CCWGG 2 cut(s) 418, 756
Bst4CI ACNGT 5 cut(s) 221, 557, 901, 967, 1000
Bst6I CTCTTC 1 cut(s) 803
BstACI GRCGYC 1 cut(s) 693
BstBAI YACGTR 2 cut(s) 523, 1125
BstBI TTCGAA 1 cut(s) 530
BstC8I GCNNGC 4 cut(s) 70, 273, 307, 322
BstDEI CTNAG 3 cut(s) 267, 473, 750
BstDSI CCRYGG 1 cut(s) 556
BstF5I GGATG 1 cut(s) 342
BstFNI CGCG 1 cut(s) 464
BstHHI GCGC 2 cut(s) 320, 466
BstKTI GATC 6 cut(s) 12, 137, 336, 718, 736, 931
BstMBI GATC 6 cut(s) 9, 134, 333, 715, 733, 928
BstMWI GCNNNNNNNGC 8 cut(s) 281, 290, 315, 569, 578, 581, 610, 619
BstNI CCWGG 2 cut(s) 418, 756
BstNSI RCATGY 3 cut(s) 92, 482, 626
BstSCI CCNGG 2 cut(s) 416, 754
BstUI CGCG 1 cut(s) 464
BstV1I GCAGC 5 cut(s) 57, 77, 296, 559, 568
BstX2I RGATCY 1 cut(s) 134
BstYI RGATCY 1 cut(s) 134
BsuRI GGCC 1 cut(s) 341
BtgI CCRYGG 1 cut(s) 556
BtrI CACGTC 1 cut(s) 592
BtsCI GGATG 1 cut(s) 342
Cac8I GCNNGC 4 cut(s) 70, 273, 307, 322
CfoI GCGC 2 cut(s) 320, 466
Cfr13I GGNCC 3 cut(s) 397, 432, 974
CseI GACGC 1 cut(s) 701
Csp6I GTAC 2 cut(s) 553, 1126
CviAII CATG 6 cut(s) 89, 353, 390, 479, 623, 1060
CviQI GTAC 2 cut(s) 553, 1126
DdeI CTNAG 3 cut(s) 267, 473, 750
DpnI GATC 6 cut(s) 11, 136, 335, 717, 735, 930
DpnII GATC 6 cut(s) 9, 134, 333, 715, 733, 928
Eam1104I CTCTTC 1 cut(s) 803
EarI CTCTTC 1 cut(s) 803
Ecl136II GAGCTC 2 cut(s) 414, 604
Eco24I GRGCYC 2 cut(s) 416, 606
Eco47I GGWCC 3 cut(s) 397, 432, 974
Eco53kI GAGCTC 2 cut(s) 414, 604
Eco57I CTGAAG 1 cut(s) 1049
Eco72I CACGTG 1 cut(s) 523
EcoICRI GAGCTC 2 cut(s) 414, 604
EcoO109I RGGNCCY 1 cut(s) 397
EcoRII CCWGG 2 cut(s) 416, 754
EcoT22I ATGCAT 1 cut(s) 624
EcoT38I GRGCYC 2 cut(s) 416, 606
FaeI CATG 6 cut(s) 92, 356, 393, 482, 626, 1063
FaqI GGGAC 2 cut(s) 348, 445
FatI CATG 6 cut(s) 88, 352, 389, 478, 622, 1059
FbaI TGATCA 2 cut(s) 715, 928
FblI GTMKAC 2 cut(s) 378, 483
Fnu4HI GCNGC 5 cut(s) 46, 66, 285, 573, 582
FokI GGATG 1 cut(s) 329
FriOI GRGCYC 2 cut(s) 416, 606
Fsp4HI GCNGC 5 cut(s) 46, 66, 285, 573, 582
FspBI CTAG 3 cut(s) 321, 491, 576
GlaI GCGC 2 cut(s) 319, 465
GluI GCNGC 5 cut(s) 46, 66, 285, 573, 582
HaeIII GGCC 1 cut(s) 341
HapII CCGG 1 cut(s) 1022
HgaI GACGC 1 cut(s) 701
HhaI GCGC 2 cut(s) 320, 466
Hin1I GRCGYC 1 cut(s) 693
Hin1II CATG 6 cut(s) 92, 356, 393, 482, 626, 1063
Hin6I GCGC 2 cut(s) 318, 464
HinP1I GCGC 2 cut(s) 318, 464
HindIII AAGCTT 1 cut(s) 534
HinfI GANTC 3 cut(s) 542, 728, 986
HpaII CCGG 1 cut(s) 1022
HphI GGTGA 5 cut(s) 95, 112, 725, 754, 897
Hpy166II GTNNAC 4 cut(s) 86, 379, 484, 553
Hpy188I TCNGA 6 cut(s) 134, 454, 541, 727, 733, 974
Hpy188III TCNNGA 2 cut(s) 179, 926
Hpy8I GTNNAC 4 cut(s) 86, 379, 484, 553
Hpy99I CGWCG 3 cut(s) 593, 695, 967
HpyAV CCTTC 6 cut(s) 23, 73, 236, 253, 753, 926
HpyCH4III ACNGT 5 cut(s) 221, 557, 901, 967, 1000
HpyCH4IV ACGT 4 cut(s) 234, 522, 591, 1124
HpyCH4V TGCA 7 cut(s) 208, 275, 393, 584, 622, 794, 1107
HpyF10VI GCNNNNNNNGC 8 cut(s) 281, 290, 315, 569, 578, 581, 610, 619
HpyF3I CTNAG 3 cut(s) 267, 473, 750
HpySE526I ACGT 4 cut(s) 234, 522, 591, 1124
Hsp92I GRCGYC 1 cut(s) 693
Hsp92II CATG 6 cut(s) 92, 356, 393, 482, 626, 1063
HspAI GCGC 2 cut(s) 318, 464
Ksp22I TGATCA 2 cut(s) 715, 928
Kzo9I GATC 6 cut(s) 9, 134, 333, 715, 733, 928
LmnI GCTCC 2 cut(s) 419, 560
Lsp1109I GCAGC 5 cut(s) 57, 77, 296, 559, 568
MaeI CTAG 3 cut(s) 321, 491, 576
MaeII ACGT 4 cut(s) 234, 522, 591, 1124
MaeIII GTNAC 1 cut(s) 260
MalI GATC 6 cut(s) 11, 136, 335, 717, 735, 930
MbiI CCGCTC 1 cut(s) 125
MboI GATC 6 cut(s) 9, 134, 333, 715, 733, 928
MboII GAAGA 2 cut(s) 820, 863
MflI RGATCY 1 cut(s) 134
MhlI GDGCHC 2 cut(s) 416, 606
MluCI AATT 1 cut(s) 437
MlyI GAGTC 1 cut(s) 737
MmeI TCCRAC 1 cut(s) 119
Mph1103I ATGCAT 1 cut(s) 624
MroXI GAANNNNTTC 1 cut(s) 90
MseI TTAA 6 cut(s) 699, 867, 1034, 1095, 1130, 1141
MslI CAYNNNNRTG 2 cut(s) 711, 1001
MspI CCGG 1 cut(s) 1022
MspR9I CCNGG 2 cut(s) 418, 756
Mva1269I GAATGC 2 cut(s) 275, 584
MvaI CCWGG 2 cut(s) 418, 756
MvnI CGCG 1 cut(s) 464
MwoI GCNNNNNNNGC 8 cut(s) 281, 290, 315, 569, 578, 581, 610, 619
NdeII GATC 6 cut(s) 9, 134, 333, 715, 733, 928
NheI GCTAGC 1 cut(s) 320
NlaIII CATG 6 cut(s) 92, 356, 393, 482, 626, 1063
NlaIV GGNNCC 3 cut(s) 218, 433, 1087
NsiI ATGCAT 1 cut(s) 624
NspI RCATGY 3 cut(s) 92, 482, 626
NspV TTCGAA 1 cut(s) 530
OliI CACNNNNGTG 2 cut(s) 711, 1001
PciI ACATGT 2 cut(s) 88, 478
PcsI WCGNNNNNNNCGW 1 cut(s) 302
PctI GAATGC 2 cut(s) 275, 584
PdmI GAANNNNTTC 1 cut(s) 90
PfeI GAWTC 2 cut(s) 542, 986
PkrI GCNGC 5 cut(s) 47, 67, 286, 574, 583
PleI GAGTC 1 cut(s) 736
PmaCI CACGTG 1 cut(s) 523
PmlI CACGTG 1 cut(s) 523
PpsI GAGTC 1 cut(s) 736
Ppu21I YACGTR 2 cut(s) 523, 1125
PpuMI RGGWCCY 1 cut(s) 397
PscI ACATGT 2 cut(s) 88, 478
Psp124BI GAGCTC 2 cut(s) 416, 606
Psp1406I AACGTT 1 cut(s) 234
Psp5II RGGWCCY 1 cut(s) 397
Psp6I CCWGG 2 cut(s) 416, 754
PspCI CACGTG 1 cut(s) 523
PspGI CCWGG 2 cut(s) 416, 754
PspN4I GGNNCC 3 cut(s) 218, 433, 1087
PspPI GGNCC 3 cut(s) 397, 432, 974
PspPPI RGGWCCY 1 cut(s) 397
PsuI RGATCY 1 cut(s) 134
RsaI GTAC 2 cut(s) 554, 1127
RsaNI GTAC 2 cut(s) 553, 1126
RseI CAYNNNNRTG 2 cut(s) 711, 1001
SacI GAGCTC 2 cut(s) 416, 606
SaqAI TTAA 6 cut(s) 699, 867, 1034, 1095, 1130, 1141
SatI GCNGC 5 cut(s) 46, 66, 285, 573, 582
Sau3AI GATC 6 cut(s) 9, 134, 333, 715, 733, 928
Sau96I GGNCC 3 cut(s) 397, 432, 974
SchI GAGTC 1 cut(s) 737
ScrFI CCNGG 2 cut(s) 418, 756
SduI GDGCHC 2 cut(s) 416, 606
SfuI TTCGAA 1 cut(s) 530
SinI GGWCC 3 cut(s) 397, 432, 974
SmiMI CAYNNNNRTG 2 cut(s) 711, 1001
SmlI CTYRAG 2 cut(s) 367, 546
SmoI CTYRAG 2 cut(s) 367, 546
Sse9I AATT 1 cut(s) 437
SsiI CCGC 2 cut(s) 123, 770
SspMI CTAG 3 cut(s) 321, 491, 576
SstI GAGCTC 2 cut(s) 416, 606
StyD4I CCNGG 2 cut(s) 416, 754
TaaI ACNGT 5 cut(s) 221, 557, 901, 967, 1000
TaiI ACGT 4 cut(s) 237, 525, 594, 1127
TaqI TCGA 5 cut(s) 180, 336, 530, 588, 962
TasI AATT 1 cut(s) 437
TfiI GAWTC 2 cut(s) 542, 986
Tru1I TTAA 6 cut(s) 699, 867, 1034, 1095, 1130, 1141
Tru9I TTAA 6 cut(s) 699, 867, 1034, 1095, 1130, 1141
TseI GCWGC 5 cut(s) 45, 65, 284, 572, 581
TspDTI ATGAA 6 cut(s) 268, 414, 504, 684, 948, 1043
VpaK11BI GGWCC 3 cut(s) 397, 432, 974
XapI RAATTY 1 cut(s) 437
XceI RCATGY 3 cut(s) 92, 482, 626
XmiI GTMKAC 2 cut(s) 378, 483
XmnI GAANNNNTTC 1 cut(s) 90
XspI CTAG 3 cut(s) 321, 491, 576
Zsp2I ATGCAT 1 cut(s) 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.