FvH4_5g14541

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000707
Physical Location & Seq
Forward (+)
8241226 .. 8241849
624 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g14541.t1

Sequence Viewer

Length: 504 bp
ATGAGGACTGATCAAGAAGCAGGTCCGTCCTCTACAGTGCCTGTAAACATCGAGTACCAACACCTGAGGAGAGGCCGTGCCGACGTCTTTTGTAAGCAGCTGACGGCATCAGATGTGAATGACCGACTTGTAGTCACCAAAGGGATGGCTACGTTGATGCCTAGGGCAAATGGCAGTGAAATCAGGGTTTTAGACGATTTTGGTGACAGTTATCGGTTCATCTTGTCTGAAAGAAATGGCCGGACGTCTCGGAAACCAACTTTCCGGCCAAGAGTTTGGAAATCCTTCATCCAACAAAGAGAAGCCAAATCTGGCGACCGTATCTATTTCTGGCCGGAGATGAACGTGGTTAAGGGTACAAGCTACCGCATCGACCTCATCCGGTTCATACATGGTGTTTCTCCTCACCCAGAAGATCTTGGAGATTCCAACGCGCCGCCTGAATCTCAAGAAGAAACCTCAGATTTGAGGACTTGGGCCGCTCATGGCGGCCTAACGCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

168

Amino Acids

19.01

Weight (kDa)

8.82

Isoelectric Point (pI)

45.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 87, 248
Acc36I ACCTGC 1 cut(s) 11
AccBSI CCGCTC 1 cut(s) 482
AccII CGCG 1 cut(s) 434
AciI CCGC 4 cut(s) 367, 437, 480, 489
AcoI YGGCCR 3 cut(s) 238, 266, 332
AcyI GRCGYC 2 cut(s) 84, 245
AfaI GTAC 2 cut(s) 56, 358
AhdI GACNNNNNGTC 1 cut(s) 131
AluBI AGCT 2 cut(s) 100, 363
AluI AGCT 2 cut(s) 100, 363
Alw26I GTCTC 1 cut(s) 252
AlwNI CAGNNNCTG 1 cut(s) 41
AoxI GGCC 6 cut(s) 73, 238, 266, 332, 477, 490
ApeKI GCWGC 1 cut(s) 97
Asp700I GAANNNNTTC 1 cut(s) 284
AspA2I CCTAGG 1 cut(s) 161
AspLEI GCGC 1 cut(s) 436
AspS9I GGNCC 2 cut(s) 23, 477
AsuHPI GGTGA 3 cut(s) 127, 215, 398
AvaII GGWCC 1 cut(s) 23
AvrII CCTAGG 1 cut(s) 161
AxyI CCTNAGG 1 cut(s) 65
BbvI GCAGC 1 cut(s) 109
BccI CCATC 1 cut(s) 139
BceAI ACGGC 2 cut(s) 60, 120
BclI TGATCA 1 cut(s) 10
BcoDI GTCTC 1 cut(s) 252
BfaI CTAG 2 cut(s) 162, 502
BfmI CTRYAG 1 cut(s) 33
BfuAI ACCTGC 1 cut(s) 11
BglII AGATCT 1 cut(s) 415
BisI GCNGC 4 cut(s) 98, 437, 480, 490
BlnI CCTAGG 1 cut(s) 161
BlsI GCNGC 4 cut(s) 99, 438, 481, 491
Bme18I GGWCC 1 cut(s) 23
BmeRI GACNNNNNGTC 1 cut(s) 131
BmgT120I GGNCC 2 cut(s) 23, 477
BmsI GCATC 3 cut(s) 116, 147, 378
BpuEI CTTGAG 1 cut(s) 432
BsaHI GRCGYC 2 cut(s) 84, 245
BsaJI CCNNGG 1 cut(s) 161
BsaWI WCCGGW 1 cut(s) 381
BsaXI ACNNNNNCTCC 2 cut(s) 61, 91
Bse21I CCTNAGG 1 cut(s) 65
BseDI CCNNGG 1 cut(s) 161
BseGI GGATG 3 cut(s) 150, 288, 378
BseMII CTCAG 2 cut(s) 56, 474
BseRI GAGGAG 2 cut(s) 82, 393
BseXI GCAGC 1 cut(s) 109
Bsh1236I CGCG 1 cut(s) 434
Bsh1285I CGRYCG 1 cut(s) 319
BshFI GGCC 6 cut(s) 75, 240, 268, 334, 479, 492
BsiEI CGRYCG 1 cut(s) 319
BsiSI CCGG 4 cut(s) 241, 265, 335, 382
BsmAI GTCTC 1 cut(s) 252
BsmBI CGTCTC 1 cut(s) 252
BsnI GGCC 6 cut(s) 75, 240, 268, 334, 479, 492
Bsp143I GATC 2 cut(s) 10, 415
BspACI CCGC 4 cut(s) 367, 437, 480, 489
BspANI GGCC 6 cut(s) 75, 240, 268, 334, 479, 492
BspCNI CTCAG 2 cut(s) 57, 473
BspFNI CGCG 1 cut(s) 434
BspMI ACCTGC 1 cut(s) 11
BsrBI CCGCTC 1 cut(s) 482
BssECI CCNNGG 1 cut(s) 161
BssMI GATC 2 cut(s) 10, 415
BssNI GRCGYC 2 cut(s) 84, 245
BssT1I CCWWGG 1 cut(s) 161
Bst4CI ACNGT 3 cut(s) 37, 209, 320
BstACI GRCGYC 2 cut(s) 84, 245
BstDEI CTNAG 2 cut(s) 65, 460
BstF5I GGATG 3 cut(s) 150, 288, 378
BstFNI CGCG 1 cut(s) 434
BstHHI GCGC 1 cut(s) 436
BstKTI GATC 2 cut(s) 13, 418
BstMAI GTCTC 1 cut(s) 252
BstMBI GATC 2 cut(s) 10, 415
BstMCI CGRYCG 1 cut(s) 319
BstSFI CTRYAG 1 cut(s) 33
BstUI CGCG 1 cut(s) 434
BstV1I GCAGC 1 cut(s) 109
BstX2I RGATCY 1 cut(s) 415
BstXI CCANNNNNNTGG 2 cut(s) 145, 276
BstYI RGATCY 1 cut(s) 415
Bsu36I CCTNAGG 1 cut(s) 65
BsuRI GGCC 6 cut(s) 75, 240, 268, 334, 479, 492
BtsCI GGATG 3 cut(s) 150, 288, 378
BtsI GCAGTG 1 cut(s) 181
BtsIMutI CAGTG 2 cut(s) 42, 181
BveI ACCTGC 1 cut(s) 11
CaiI CAGNNNCTG 1 cut(s) 41
CfoI GCGC 1 cut(s) 436
Cfr13I GGNCC 2 cut(s) 23, 477
Csp6I GTAC 2 cut(s) 55, 357
CviAII CATG 2 cut(s) 392, 485
CviQI GTAC 2 cut(s) 55, 357
DdeI CTNAG 2 cut(s) 65, 460
DpnI GATC 2 cut(s) 12, 417
DpnII GATC 2 cut(s) 10, 415
DriI GACNNNNNGTC 1 cut(s) 131
EaeI YGGCCR 3 cut(s) 238, 266, 332
Eam1105I GACNNNNNGTC 1 cut(s) 131
Eco130I CCWWGG 1 cut(s) 161
Eco47I GGWCC 1 cut(s) 23
Eco81I CCTNAGG 1 cut(s) 65
EcoT14I CCWWGG 1 cut(s) 161
ErhI CCWWGG 1 cut(s) 161
Esp3I CGTCTC 1 cut(s) 252
FaeI CATG 2 cut(s) 395, 488
FaiI YATR 3 cut(s) 389, 393, 486
FatI CATG 2 cut(s) 391, 484
FbaI TGATCA 1 cut(s) 10
Fnu4HI GCNGC 4 cut(s) 98, 437, 480, 490
FokI GGATG 3 cut(s) 157, 275, 365
Fsp4HI GCNGC 4 cut(s) 98, 437, 480, 490
FspBI CTAG 2 cut(s) 162, 502
GlaI GCGC 1 cut(s) 435
GluI GCNGC 4 cut(s) 98, 437, 480, 490
HaeIII GGCC 6 cut(s) 75, 240, 268, 334, 479, 492
HapII CCGG 4 cut(s) 241, 265, 335, 382
HhaI GCGC 1 cut(s) 436
Hin1I GRCGYC 2 cut(s) 84, 245
Hin1II CATG 2 cut(s) 395, 488
Hin6I GCGC 1 cut(s) 434
HinP1I GCGC 1 cut(s) 434
HinfI GANTC 2 cut(s) 425, 443
HpaII CCGG 4 cut(s) 241, 265, 335, 382
HphI GGTGA 3 cut(s) 127, 215, 398
Hpy166II GTNNAC 1 cut(s) 46
Hpy188I TCNGA 4 cut(s) 112, 229, 252, 463
Hpy188III TCNNGA 2 cut(s) 14, 449
Hpy8I GTNNAC 1 cut(s) 46
Hpy99I CGWCG 1 cut(s) 86
HpyAV CCTTC 1 cut(s) 295
HpyCH4III ACNGT 3 cut(s) 37, 209, 320
HpyCH4IV ACGT 4 cut(s) 84, 152, 245, 345
HpyF3I CTNAG 2 cut(s) 65, 460
HpySE526I ACGT 4 cut(s) 84, 152, 245, 345
Hsp92I GRCGYC 2 cut(s) 84, 245
Hsp92II CATG 2 cut(s) 395, 488
HspAI GCGC 1 cut(s) 434
Ksp22I TGATCA 1 cut(s) 10
Kzo9I GATC 2 cut(s) 10, 415
Lsp1109I GCAGC 1 cut(s) 109
LweI GCATC 3 cut(s) 116, 147, 378
MaeI CTAG 2 cut(s) 162, 502
MaeII ACGT 4 cut(s) 84, 152, 245, 345
MaeIII GTNAC 2 cut(s) 133, 203
MalI GATC 2 cut(s) 12, 417
MbiI CCGCTC 1 cut(s) 482
MboI GATC 2 cut(s) 10, 415
MboII GAAGA 2 cut(s) 425, 464
MflI RGATCY 1 cut(s) 415
MmeI TCCRAC 2 cut(s) 316, 453
MnlI CCTC 7 cut(s) 40, 60, 65, 386, 414, 462, 469
MroXI GAANNNNTTC 1 cut(s) 284
MseI TTAA 1 cut(s) 351
MspA1I CMGCKG 1 cut(s) 100
MspI CCGG 4 cut(s) 241, 265, 335, 382
MvnI CGCG 1 cut(s) 434
NdeII GATC 2 cut(s) 10, 415
NlaIII CATG 2 cut(s) 395, 488
NmuCI GTSAC 2 cut(s) 133, 203
PdmI GAANNNNTTC 1 cut(s) 284
PfeI GAWTC 2 cut(s) 425, 443
PkrI GCNGC 4 cut(s) 99, 438, 481, 491
PspPI GGNCC 2 cut(s) 23, 477
PstNI CAGNNNCTG 1 cut(s) 41
PsuI RGATCY 1 cut(s) 415
PvuII CAGCTG 1 cut(s) 100
RsaI GTAC 2 cut(s) 56, 358
RsaNI GTAC 2 cut(s) 55, 357
SaqAI TTAA 1 cut(s) 351
SatI GCNGC 4 cut(s) 98, 437, 480, 490
Sau3AI GATC 2 cut(s) 10, 415
Sau96I GGNCC 2 cut(s) 23, 477
SfaNI GCATC 3 cut(s) 116, 147, 378
SfcI CTRYAG 1 cut(s) 33
SinI GGWCC 1 cut(s) 23
SmlI CTYRAG 1 cut(s) 447
SmoI CTYRAG 1 cut(s) 447
SsiI CCGC 4 cut(s) 367, 437, 480, 489
SspMI CTAG 2 cut(s) 162, 502
StyI CCWWGG 1 cut(s) 161
TaaI ACNGT 3 cut(s) 37, 209, 320
TaiI ACGT 4 cut(s) 87, 155, 248, 348
TaqI TCGA 2 cut(s) 51, 372
TaqII GACCGA 1 cut(s) 138
TauI GCSGC 3 cut(s) 439, 482, 492
TfiI GAWTC 2 cut(s) 425, 443
Tru1I TTAA 1 cut(s) 351
Tru9I TTAA 1 cut(s) 351
TscAI CASTG 2 cut(s) 42, 181
TseFI GTSAC 2 cut(s) 133, 203
TseI GCWGC 1 cut(s) 97
Tsp45I GTSAC 2 cut(s) 133, 203
TspDTI ATGAA 4 cut(s) 208, 277, 356, 376
TspGWI ACGGA 1 cut(s) 15
TspRI CASTG 2 cut(s) 42, 181
VpaK11BI GGWCC 1 cut(s) 23
XmaJI CCTAGG 1 cut(s) 161
XmnI GAANNNNTTC 1 cut(s) 284
XspI CTAG 2 cut(s) 162, 502
ZraI GACGTC 2 cut(s) 85, 246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.