Rh1DG452600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000707
Physical Location & Seq
Reverse (-)
65993500 .. 65993874
375 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG452600.1

Sequence Viewer

Length: 375 bp
ATGGAGCTTGAGTACCGGAGAGGCCGTGCTGTGAAAAAGCTGACCTCATCAGATGTGGGTAGCAGACTCGTAGTGACTAATGAGATGCTTCACATGCTGCCTCGAGCAGATCCAGAACACGACACTCCAATCAGAGTGTTGGACGATAAGGGCGACGAGTATCAGTTCTACTTGTCTATAAGAAAGGGCCCAAATCCCAAACCAGTTTTCCAGGCCAAAGGATGGAAAGCATTCATCAAAAAACGAGGAGCCAAACTTGACGACTACATCTGTTTCTGGCCAGAGGTGAATGCAATTAAGCGTACAGATTACCGCATCAAGTTAGTTCCATTCACACTTTATCTTTCCCCCAACCCAGAAGGTCTTTCATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

124

Amino Acids

14.37

Weight (kDa)

9.49

Isoelectric Point (pI)

22.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 11 - 92 6.7e-06 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 222
AciI CCGC 1 cut(s) 313
AclWI GGATC 1 cut(s) 104
AcoI YGGCCR 1 cut(s) 278
AfaI GTAC 2 cut(s) 14, 304
AfiI CCNNNNNNNGG 1 cut(s) 222
AjnI CCWGG 1 cut(s) 210
AjuI GAANNNNNNNTTGG 2 cut(s) 191, 223
AluBI AGCT 2 cut(s) 7, 40
AluI AGCT 2 cut(s) 7, 40
AlwI GGATC 1 cut(s) 104
Ama87I CYCGRG 1 cut(s) 102
AoxI GGCC 4 cut(s) 22, 187, 213, 278
ApaI GGGCCC 1 cut(s) 191
ApeKI GCWGC 1 cut(s) 97
Asp700I GAANNNNTTC 1 cut(s) 230
AspS9I GGNCC 2 cut(s) 187, 188
AsuHPI GGTGA 1 cut(s) 298
AvaI CYCGRG 1 cut(s) 102
BaeGI GKGCMC 1 cut(s) 191
BalI TGGCCA 1 cut(s) 280
BanII GRGCYC 1 cut(s) 191
BbvI GCAGC 1 cut(s) 84
BccI CCATC 1 cut(s) 216
BceAI ACGGC 1 cut(s) 9
BciT130I CCWGG 1 cut(s) 212
BisI GCNGC 1 cut(s) 98
BlsI GCNGC 1 cut(s) 99
Bme1390I CCNGG 1 cut(s) 212
BmeT110I CYCGRG 1 cut(s) 102
BmgT120I GGNCC 2 cut(s) 187, 188
BmiI GGNNCC 2 cut(s) 189, 250
BmrFI CCNGG 1 cut(s) 212
BmsI GCATC 2 cut(s) 75, 324
BpuEI CTTGAG 1 cut(s) 29
BsaWI WCCGGW 1 cut(s) 15
BsaXI ACNNNNNCTCC 3 cut(s) 10, 26, 40
Bsc4I CCNNNNNNNGG 1 cut(s) 222
Bse1I ACTGG 1 cut(s) 203
BseBI CCWGG 1 cut(s) 212
BseGI GGATG 1 cut(s) 227
BseLI CCNNNNNNNGG 1 cut(s) 222
BseNI ACTGG 1 cut(s) 203
BseRI GAGGAG 1 cut(s) 261
BseSI GKGCMC 1 cut(s) 191
BseXI GCAGC 1 cut(s) 84
BshFI GGCC 4 cut(s) 24, 189, 215, 280
BsiHKCI CYCGRG 1 cut(s) 102
BsiSI CCGG 1 cut(s) 16
BslI CCNNNNNNNGG 1 cut(s) 222
BsmI GAATGC 2 cut(s) 230, 295
BsnI GGCC 4 cut(s) 24, 189, 215, 280
BsoBI CYCGRG 1 cut(s) 102
Bsp120I GGGCCC 1 cut(s) 187
Bsp1286I GDGCHC 1 cut(s) 191
Bsp143I GATC 1 cut(s) 109
BspACI CCGC 1 cut(s) 313
BspANI GGCC 4 cut(s) 24, 189, 215, 280
BspLI GGNNCC 2 cut(s) 189, 250
BspPI GGATC 1 cut(s) 104
BsrI ACTGG 1 cut(s) 203
BssMI GATC 1 cut(s) 109
Bst2UI CCWGG 1 cut(s) 212
BstF5I GGATG 1 cut(s) 227
BstKTI GATC 1 cut(s) 112
BstMBI GATC 1 cut(s) 109
BstMWI GCNNNNNNNGC 1 cut(s) 94
BstNI CCWGG 1 cut(s) 212
BstNSI RCATGY 1 cut(s) 97
BstSCI CCNGG 1 cut(s) 210
BstSLI GKGCMC 1 cut(s) 191
BstV1I GCAGC 1 cut(s) 84
BstX2I RGATCY 1 cut(s) 109
BstYI RGATCY 1 cut(s) 109
BsuRI GGCC 4 cut(s) 24, 189, 215, 280
BtsCI GGATG 1 cut(s) 227
Cfr13I GGNCC 2 cut(s) 187, 188
Csp6I GTAC 2 cut(s) 13, 303
CviAII CATG 1 cut(s) 94
CviJI RGCY 7 cut(s) 7, 24, 40, 189, 215, 251, 280
CviKI_1 RGCY 7 cut(s) 7, 24, 40, 189, 215, 251, 280
CviQI GTAC 2 cut(s) 13, 303
DpnI GATC 1 cut(s) 111
DpnII GATC 1 cut(s) 109
EaeI YGGCCR 1 cut(s) 278
Eco24I GRGCYC 1 cut(s) 191
Eco88I CYCGRG 1 cut(s) 102
EcoO109I RGGNCCY 1 cut(s) 187
EcoRII CCWGG 1 cut(s) 210
EcoT38I GRGCYC 1 cut(s) 191
FaeI CATG 1 cut(s) 97
FaiI YATR 2 cut(s) 95, 179
FatI CATG 1 cut(s) 93
Fnu4HI GCNGC 1 cut(s) 98
FokI GGATG 1 cut(s) 234
FriOI GRGCYC 1 cut(s) 191
Fsp4HI GCNGC 1 cut(s) 98
GluI GCNGC 1 cut(s) 98
HaeIII GGCC 4 cut(s) 24, 189, 215, 280
HapII CCGG 1 cut(s) 16
Hin1II CATG 1 cut(s) 97
HinfI GANTC 1 cut(s) 66
HpaII CCGG 1 cut(s) 16
HphI GGTGA 1 cut(s) 298
Hpy188I TCNGA 2 cut(s) 52, 134
Hpy188III TCNNGA 1 cut(s) 113
Hpy99I CGWCG 1 cut(s) 158
HpyAV CCTTC 1 cut(s) 353
HpyCH4V TGCA 1 cut(s) 293
HpyF10VI GCNNNNNNNGC 1 cut(s) 94
Hsp92II CATG 1 cut(s) 97
Kzo9I GATC 1 cut(s) 109
LmnI GCTCC 2 cut(s) 4, 248
LpnPI CCDG 8 cut(s) 29, 126, 197, 216, 224, 262, 294, 369
Lsp1109I GCAGC 1 cut(s) 84
LweI GCATC 2 cut(s) 75, 324
MaeIII GTNAC 1 cut(s) 73
MalI GATC 1 cut(s) 111
MboI GATC 1 cut(s) 109
MflI RGATCY 1 cut(s) 109
MhlI GDGCHC 1 cut(s) 191
MlsI TGGCCA 1 cut(s) 280
MluCI AATT 1 cut(s) 294
MluNI TGGCCA 1 cut(s) 280
MlyI GAGTC 1 cut(s) 60
MmeI TCCRAC 1 cut(s) 120
MnlI CCTC 5 cut(s) 14, 55, 111, 239, 277
Mox20I TGGCCA 1 cut(s) 280
MroXI GAANNNNTTC 1 cut(s) 230
MscI TGGCCA 1 cut(s) 280
MseI TTAA 2 cut(s) 297, 373
Msp20I TGGCCA 1 cut(s) 280
MspI CCGG 1 cut(s) 16
MspR9I CCNGG 1 cut(s) 212
Mva1269I GAATGC 2 cut(s) 230, 295
MvaI CCWGG 1 cut(s) 212
MwoI GCNNNNNNNGC 1 cut(s) 94
NdeII GATC 1 cut(s) 109
NlaIII CATG 1 cut(s) 97
NlaIV GGNNCC 2 cut(s) 189, 250
NmuCI GTSAC 1 cut(s) 73
NspI RCATGY 1 cut(s) 97
PaeR7I CTCGAG 1 cut(s) 102
PcsI WCGNNNNNNNCGW 1 cut(s) 150
PctI GAATGC 2 cut(s) 230, 295
PdmI GAANNNNTTC 1 cut(s) 230
PflMI CCANNNNNTGG 1 cut(s) 222
PkrI GCNGC 1 cut(s) 99
PleI GAGTC 1 cut(s) 60
PpsI GAGTC 1 cut(s) 60
Psp6I CCWGG 1 cut(s) 210
PspGI CCWGG 1 cut(s) 210
PspN4I GGNNCC 2 cut(s) 189, 250
PspOMI GGGCCC 1 cut(s) 187
PspPI GGNCC 2 cut(s) 187, 188
PspXI VCTCGAGB 1 cut(s) 102
PsuI RGATCY 1 cut(s) 109
RsaI GTAC 2 cut(s) 14, 304
RsaNI GTAC 2 cut(s) 13, 303
SaqAI TTAA 2 cut(s) 297, 373
SatI GCNGC 1 cut(s) 98
Sau3AI GATC 1 cut(s) 109
Sau96I GGNCC 2 cut(s) 187, 188
SchI GAGTC 1 cut(s) 60
ScrFI CCNGG 1 cut(s) 212
SduI GDGCHC 1 cut(s) 191
SetI ASST 5 cut(s) 9, 42, 47, 288, 364
SfaNI GCATC 2 cut(s) 75, 324
Sfr274I CTCGAG 1 cut(s) 102
SlaI CTCGAG 1 cut(s) 102
SmlI CTYRAG 2 cut(s) 8, 102
SmoI CTYRAG 2 cut(s) 8, 102
Sse9I AATT 1 cut(s) 294
SsiI CCGC 1 cut(s) 313
StyD4I CCNGG 1 cut(s) 210
TaqI TCGA 1 cut(s) 103
TasI AATT 1 cut(s) 294
Tru1I TTAA 2 cut(s) 297, 373
Tru9I TTAA 2 cut(s) 297, 373
TseFI GTSAC 1 cut(s) 73
TseI GCWGC 1 cut(s) 97
Tsp45I GTSAC 1 cut(s) 73
TspDTI ATGAA 2 cut(s) 223, 357
Van91I CCANNNNNTGG 1 cut(s) 222
XceI RCATGY 1 cut(s) 97
XhoI CTCGAG 1 cut(s) 102
XmnI GAANNNNTTC 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.