RLG00000005159

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000707
Physical Location & Seq
Forward (+)
65541231 .. 65541680
450 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005159

Sequence Viewer

Length: 450 bp
ATGGAGCTCGAGTACCTGAAATGCCTTCCAAAGTTTGTGAAAGAGCTGAAGCCGACAGATGTGGATAGCAGACTCGTAATGACTAATGAGATCCTTCTCATGGTACCTCATGCAGGTCCAGAACATGACACTCCAATCAGAGTGTTGGACGATGAGGGTGACGACTATCTGTTCTACTTGTCTCTAAGAAAGGGCCCACATCTCAAACCAGTTTTCCAGGCCAAAGGATGGCAAACATTCATAAAAAAACGAGGAGCGAAAATTGGCGACTTCATCTGTTTCTGGCCAGAGGTGAATGCAATTAAGCGTACAGATTACCGCATCAAGTTAATTCCATTCACACCTCATCTTTCCCCCAACCCAGAAGGTCTTTCATCTTCCAGGCCATCTGCATCTGCAGAGGAACATGAAACCCTAGAGAATTGGGCGGCTAGCGGACTAGCACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

150

Amino Acids

16.96

Weight (kDa)

6.2

Isoelectric Point (pI)

30.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 104
Acc65I GGTACC 1 cut(s) 103
AccB1I GGYRCC 1 cut(s) 103
AccB7I CCANNNNNTGG 1 cut(s) 228
AciI CCGC 3 cut(s) 319, 428, 435
AclWI GGATC 1 cut(s) 85
AcoI YGGCCR 1 cut(s) 284
AcuI CTGAAG 1 cut(s) 68
AfaI GTAC 3 cut(s) 14, 105, 310
AfiI CCNNNNNNNGG 3 cut(s) 100, 113, 228
AjnI CCWGG 2 cut(s) 216, 380
AluBI AGCT 2 cut(s) 7, 46
AluI AGCT 2 cut(s) 7, 46
Alw21I GWGCWC 1 cut(s) 9
Alw26I GTCTC 1 cut(s) 186
AlwI GGATC 1 cut(s) 85
Ama87I CYCGRG 1 cut(s) 8
AoxI GGCC 4 cut(s) 193, 219, 284, 383
ApaI GGGCCC 1 cut(s) 197
Asp718I GGTACC 1 cut(s) 103
AspS9I GGNCC 3 cut(s) 116, 193, 194
AsuHPI GGTGA 2 cut(s) 170, 304
AsuNHI GCTAGC 1 cut(s) 431
AvaI CYCGRG 1 cut(s) 8
AvaII GGWCC 1 cut(s) 116
BaeGI GKGCMC 1 cut(s) 197
BalI TGGCCA 1 cut(s) 286
BanI GGYRCC 1 cut(s) 103
BanII GRGCYC 2 cut(s) 9, 197
Bbv12I GWGCWC 1 cut(s) 9
BccI CCATC 2 cut(s) 222, 394
BciT130I CCWGG 2 cut(s) 218, 382
BcoDI GTCTC 1 cut(s) 186
BfaI CTAG 4 cut(s) 416, 432, 440, 448
BfmI CTRYAG 1 cut(s) 396
BfuAI ACCTGC 1 cut(s) 104
BisI GCNGC 1 cut(s) 429
BlsI GCNGC 1 cut(s) 430
Bme1390I CCNGG 2 cut(s) 218, 382
Bme18I GGWCC 1 cut(s) 116
BmeT110I CYCGRG 1 cut(s) 8
BmgT120I GGNCC 3 cut(s) 116, 193, 194
BmiI GGNNCC 2 cut(s) 105, 195
BmrFI CCNGG 2 cut(s) 218, 382
BmsI GCATC 2 cut(s) 330, 401
BmtI GCTAGC 1 cut(s) 435
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 3 cut(s) 100, 113, 228
Bse1I ACTGG 1 cut(s) 209
BseBI CCWGG 2 cut(s) 218, 382
BseGI GGATG 1 cut(s) 233
BseLI CCNNNNNNNGG 3 cut(s) 100, 113, 228
BseNI ACTGG 1 cut(s) 209
BseRI GAGGAG 1 cut(s) 267
BseSI GKGCMC 1 cut(s) 197
BshFI GGCC 4 cut(s) 195, 221, 286, 385
BshNI GGYRCC 1 cut(s) 103
BsiHKAI GWGCWC 1 cut(s) 9
BsiHKCI CYCGRG 1 cut(s) 8
BslI CCNNNNNNNGG 3 cut(s) 100, 113, 228
BsmAI GTCTC 1 cut(s) 186
BsmI GAATGC 1 cut(s) 301
BsnI GGCC 4 cut(s) 195, 221, 286, 385
BsoBI CYCGRG 1 cut(s) 8
Bsp120I GGGCCC 1 cut(s) 193
Bsp1286I GDGCHC 2 cut(s) 9, 197
Bsp143I GATC 1 cut(s) 90
BspACI CCGC 3 cut(s) 319, 428, 435
BspANI GGCC 4 cut(s) 195, 221, 286, 385
BspLI GGNNCC 2 cut(s) 105, 195
BspMAI CTGCAG 1 cut(s) 400
BspMI ACCTGC 1 cut(s) 104
BspOI GCTAGC 1 cut(s) 435
BspPI GGATC 1 cut(s) 85
BspT107I GGYRCC 1 cut(s) 103
BsrI ACTGG 1 cut(s) 209
BssMI GATC 1 cut(s) 90
Bst2UI CCWGG 2 cut(s) 218, 382
BstC8I GCNNGC 1 cut(s) 433
BstDEI CTNAG 1 cut(s) 185
BstENI CCTNNNNNAGG 1 cut(s) 111
BstF5I GGATG 1 cut(s) 233
BstKTI GATC 1 cut(s) 93
BstMAI GTCTC 1 cut(s) 186
BstMBI GATC 1 cut(s) 90
BstNI CCWGG 2 cut(s) 218, 382
BstSCI CCNGG 2 cut(s) 216, 380
BstSFI CTRYAG 1 cut(s) 396
BstSLI GKGCMC 1 cut(s) 197
BstX2I RGATCY 1 cut(s) 90
BstYI RGATCY 1 cut(s) 90
BsuRI GGCC 4 cut(s) 195, 221, 286, 385
BtsCI GGATG 1 cut(s) 233
BveI ACCTGC 1 cut(s) 104
Cac8I GCNNGC 1 cut(s) 433
Cfr13I GGNCC 3 cut(s) 116, 193, 194
Csp6I GTAC 3 cut(s) 13, 104, 309
CviAII CATG 4 cut(s) 100, 110, 125, 407
CviJI RGCY 8 cut(s) 7, 46, 52, 195, 221, 286, 385, 431
CviKI_1 RGCY 8 cut(s) 7, 46, 52, 195, 221, 286, 385, 431
CviQI GTAC 3 cut(s) 13, 104, 309
DdeI CTNAG 1 cut(s) 185
DpnI GATC 1 cut(s) 92
DpnII GATC 1 cut(s) 90
EaeI YGGCCR 1 cut(s) 284
Ecl136II GAGCTC 1 cut(s) 7
Eco24I GRGCYC 2 cut(s) 9, 197
Eco47I GGWCC 1 cut(s) 116
Eco53kI GAGCTC 1 cut(s) 7
Eco57I CTGAAG 1 cut(s) 68
Eco88I CYCGRG 1 cut(s) 8
EcoICRI GAGCTC 1 cut(s) 7
EcoNI CCTNNNNNAGG 1 cut(s) 111
EcoO109I RGGNCCY 1 cut(s) 193
EcoRII CCWGG 2 cut(s) 216, 380
EcoT38I GRGCYC 2 cut(s) 9, 197
FaeI CATG 4 cut(s) 103, 113, 128, 410
FaiI YATR 5 cut(s) 101, 111, 126, 242, 408
FatI CATG 4 cut(s) 99, 109, 124, 406
Fnu4HI GCNGC 1 cut(s) 429
FokI GGATG 1 cut(s) 240
FriOI GRGCYC 2 cut(s) 9, 197
Fsp4HI GCNGC 1 cut(s) 429
FspBI CTAG 4 cut(s) 416, 432, 440, 448
GluI GCNGC 1 cut(s) 429
HaeIII GGCC 4 cut(s) 195, 221, 286, 385
Hin1II CATG 4 cut(s) 103, 113, 128, 410
HinfI GANTC 1 cut(s) 72
HphI GGTGA 2 cut(s) 170, 304
Hpy188I TCNGA 1 cut(s) 140
Hpy188III TCNNGA 1 cut(s) 119
HpyAV CCTTC 3 cut(s) 35, 104, 359
HpyCH4V TGCA 4 cut(s) 113, 299, 392, 398
HpyF3I CTNAG 1 cut(s) 185
Hsp92II CATG 4 cut(s) 103, 113, 128, 410
KpnI GGTACC 1 cut(s) 107
Kzo9I GATC 1 cut(s) 90
LmnI GCTCC 2 cut(s) 4, 254
LweI GCATC 2 cut(s) 330, 401
MaeI CTAG 4 cut(s) 416, 432, 440, 448
MaeIII GTNAC 1 cut(s) 158
MalI GATC 1 cut(s) 92
MboI GATC 1 cut(s) 90
MboII GAAGA 1 cut(s) 369
MflI RGATCY 1 cut(s) 90
MhlI GDGCHC 2 cut(s) 9, 197
MlsI TGGCCA 1 cut(s) 286
MluCI AATT 4 cut(s) 261, 300, 330, 421
MluNI TGGCCA 1 cut(s) 286
MlyI GAGTC 1 cut(s) 66
MmeI TCCRAC 1 cut(s) 126
MnlI CCTC 6 cut(s) 117, 148, 245, 283, 354, 394
Mox20I TGGCCA 1 cut(s) 286
MscI TGGCCA 1 cut(s) 286
MseI TTAA 2 cut(s) 303, 329
Msp20I TGGCCA 1 cut(s) 286
MspR9I CCNGG 2 cut(s) 218, 382
Mva1269I GAATGC 1 cut(s) 301
MvaI CCWGG 2 cut(s) 218, 382
NdeII GATC 1 cut(s) 90
NheI GCTAGC 1 cut(s) 431
NlaIII CATG 4 cut(s) 103, 113, 128, 410
NlaIV GGNNCC 2 cut(s) 105, 195
NmuCI GTSAC 1 cut(s) 158
PaeR7I CTCGAG 1 cut(s) 8
PctI GAATGC 1 cut(s) 301
PflMI CCANNNNNTGG 1 cut(s) 228
PkrI GCNGC 1 cut(s) 430
PleI GAGTC 1 cut(s) 66
PpsI GAGTC 1 cut(s) 66
Psp124BI GAGCTC 1 cut(s) 9
Psp6I CCWGG 2 cut(s) 216, 380
PspGI CCWGG 2 cut(s) 216, 380
PspN4I GGNNCC 2 cut(s) 105, 195
PspOMI GGGCCC 1 cut(s) 193
PspPI GGNCC 3 cut(s) 116, 193, 194
PspXI VCTCGAGB 1 cut(s) 8
PstI CTGCAG 1 cut(s) 400
PsuI RGATCY 1 cut(s) 90
RsaI GTAC 3 cut(s) 14, 105, 310
RsaNI GTAC 3 cut(s) 13, 104, 309
SacI GAGCTC 1 cut(s) 9
SaqAI TTAA 2 cut(s) 303, 329
SatI GCNGC 1 cut(s) 429
Sau3AI GATC 1 cut(s) 90
Sau96I GGNCC 3 cut(s) 116, 193, 194
SchI GAGTC 1 cut(s) 66
ScrFI CCNGG 2 cut(s) 218, 382
SduI GDGCHC 2 cut(s) 9, 197
SetI ASST 8 cut(s) 9, 18, 48, 109, 118, 294, 346, 370
SfaNI GCATC 2 cut(s) 330, 401
SfcI CTRYAG 1 cut(s) 396
Sfr274I CTCGAG 1 cut(s) 8
SinI GGWCC 1 cut(s) 116
SlaI CTCGAG 1 cut(s) 8
SmlI CTYRAG 1 cut(s) 8
SmoI CTYRAG 1 cut(s) 8
Sse9I AATT 4 cut(s) 261, 300, 330, 421
SsiI CCGC 3 cut(s) 319, 428, 435
SspMI CTAG 4 cut(s) 416, 432, 440, 448
SstI GAGCTC 1 cut(s) 9
StyD4I CCNGG 2 cut(s) 216, 380
TaqI TCGA 1 cut(s) 9
TasI AATT 4 cut(s) 261, 300, 330, 421
TauI GCSGC 1 cut(s) 431
Tru1I TTAA 2 cut(s) 303, 329
Tru9I TTAA 2 cut(s) 303, 329
TseFI GTSAC 1 cut(s) 158
Tsp45I GTSAC 1 cut(s) 158
TspDTI ATGAA 4 cut(s) 229, 262, 363, 423
Van91I CCANNNNNTGG 1 cut(s) 228
VpaK11BI GGWCC 1 cut(s) 116
XagI CCTNNNNNAGG 1 cut(s) 111
XhoI CTCGAG 1 cut(s) 8
XspI CTAG 4 cut(s) 416, 432, 440, 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.