Rh7DG047300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000707
Physical Location & Seq
Reverse (-)
3648298 .. 3648777
480 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG047300.1

Sequence Viewer

Length: 480 bp
ATGGAGCTCCAGTACCTGGAAGGCCGTCCAGTGTTTGTGAAACATCTGAAGCAAACAGATGTGGATAGCAGACTGGTACTGACTACTGAGATGCTTCACACGATCATGCCTCCTCCTGATCCAAAACATGACACTCCAATCAGAGTCTTGGATGATAAGGGTGACGTCTATCAGTTCGACTTGTCTGTAAGAAAGGGCCCACATCTCAAACCAGTTTTCCAGGCCAAAGAATGGCAAGCATTCATCAGAAAACGAAGAGCCAAAGAAAAGGACACCATCTATTTCTGGCAAGAGGTGAATGCAATTAATCATACAGATTACCGCATCATGTTACTAACATGTTATCTTTCCCCCAAGCCAGAAGGTCTTTCATCTCTCGAGCCATCTGCACCTGCAGATGAACATGAAAAGCTAGCTATGAAGGCTTGGGCGGCTCACGGCGGTCTAGCACTAAGCACTGTAGCGAATGATCACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

159

Amino Acids

18.27

Weight (kDa)

6.7

Isoelectric Point (pI)

30.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 400
AatII GACGTC 1 cut(s) 168
Acc36I ACCTGC 1 cut(s) 400
AccB7I CCANNNNNTGG 2 cut(s) 16, 231
AciI CCGC 3 cut(s) 322, 431, 441
AclWI GGATC 1 cut(s) 113
AcuI CTGAAG 1 cut(s) 68
AcyI GRCGYC 1 cut(s) 165
AfaI GTAC 2 cut(s) 14, 78
AfiI CCNNNNNNNGG 2 cut(s) 16, 231
AflIII ACRYGT 1 cut(s) 338
AjnI CCWGG 2 cut(s) 15, 219
AluBI AGCT 3 cut(s) 7, 412, 416
AluI AGCT 3 cut(s) 7, 412, 416
Alw21I GWGCWC 1 cut(s) 9
AlwI GGATC 1 cut(s) 113
AlwNI CAGNNNCTG 1 cut(s) 16
Ama87I CYCGRG 1 cut(s) 377
AoxI GGCC 3 cut(s) 22, 196, 222
ApaI GGGCCC 1 cut(s) 200
AseI ATTAAT 1 cut(s) 306
AspS9I GGNCC 2 cut(s) 196, 197
AsuHPI GGTGA 2 cut(s) 173, 307
AsuNHI GCTAGC 1 cut(s) 412
AvaI CYCGRG 1 cut(s) 377
BaeGI GKGCMC 1 cut(s) 200
BanII GRGCYC 2 cut(s) 9, 200
Bbv12I GWGCWC 1 cut(s) 9
BccI CCATC 2 cut(s) 284, 391
BceAI ACGGC 2 cut(s) 9, 454
BcgI CGANNNNNNTGC 2 cut(s) 368, 402
BciT130I CCWGG 2 cut(s) 17, 221
BclI TGATCA 1 cut(s) 469
BfaI CTAG 2 cut(s) 413, 446
BfmI CTRYAG 2 cut(s) 393, 459
BfuAI ACCTGC 1 cut(s) 400
BisI GCNGC 1 cut(s) 432
BlsI GCNGC 1 cut(s) 433
Bme1390I CCNGG 2 cut(s) 17, 221
BmeT110I CYCGRG 1 cut(s) 377
BmgT120I GGNCC 2 cut(s) 196, 197
BmiI GGNNCC 1 cut(s) 198
BmrFI CCNGG 2 cut(s) 17, 221
BmsI GCATC 2 cut(s) 81, 333
BmtI GCTAGC 1 cut(s) 416
BsaHI GRCGYC 1 cut(s) 165
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 2 cut(s) 16, 231
Bse1I ACTGG 4 cut(s) 10, 29, 78, 212
BseBI CCWGG 2 cut(s) 17, 221
BseGI GGATG 1 cut(s) 157
BseLI CCNNNNNNNGG 2 cut(s) 16, 231
BseMII CTCAG 1 cut(s) 78
BseNI ACTGG 4 cut(s) 10, 29, 78, 212
BseRI GAGGAG 1 cut(s) 102
BseSI GKGCMC 1 cut(s) 200
BsgI GTGCAG 1 cut(s) 372
BshFI GGCC 3 cut(s) 24, 198, 224
BsiHKAI GWGCWC 1 cut(s) 9
BsiHKCI CYCGRG 1 cut(s) 377
BslI CCNNNNNNNGG 2 cut(s) 16, 231
BsmI GAATGC 2 cut(s) 239, 304
BsnI GGCC 3 cut(s) 24, 198, 224
BsoBI CYCGRG 1 cut(s) 377
Bsp120I GGGCCC 1 cut(s) 196
Bsp1286I GDGCHC 2 cut(s) 9, 200
Bsp143I GATC 3 cut(s) 102, 118, 469
BspACI CCGC 3 cut(s) 322, 431, 441
BspANI GGCC 3 cut(s) 24, 198, 224
BspCNI CTCAG 1 cut(s) 79
BspLI GGNNCC 1 cut(s) 198
BspMAI CTGCAG 1 cut(s) 397
BspMI ACCTGC 1 cut(s) 400
BspOI GCTAGC 1 cut(s) 416
BspPI GGATC 1 cut(s) 113
BspQI GCTCTTC 1 cut(s) 250
BsrI ACTGG 4 cut(s) 10, 29, 78, 212
BssMI GATC 3 cut(s) 102, 118, 469
BssNI GRCGYC 1 cut(s) 165
Bst2UI CCWGG 2 cut(s) 17, 221
Bst4CI ACNGT 1 cut(s) 460
Bst6I CTCTTC 1 cut(s) 250
BstACI GRCGYC 1 cut(s) 165
BstC8I GCNNGC 2 cut(s) 237, 414
BstDEI CTNAG 2 cut(s) 87, 452
BstF5I GGATG 1 cut(s) 157
BstKTI GATC 3 cut(s) 105, 121, 472
BstMBI GATC 3 cut(s) 102, 118, 469
BstMWI GCNNNNNNNGC 2 cut(s) 422, 431
BstNI CCWGG 2 cut(s) 17, 221
BstNSI RCATGY 1 cut(s) 342
BstSCI CCNGG 2 cut(s) 15, 219
BstSFI CTRYAG 2 cut(s) 393, 459
BstSLI GKGCMC 1 cut(s) 200
BsuRI GGCC 3 cut(s) 24, 198, 224
BtsCI GGATG 1 cut(s) 157
BtsIMutI CAGTG 2 cut(s) 36, 456
BveI ACCTGC 1 cut(s) 400
Cac8I GCNNGC 2 cut(s) 237, 414
CaiI CAGNNNCTG 1 cut(s) 16
Cfr13I GGNCC 2 cut(s) 196, 197
Csp6I GTAC 2 cut(s) 13, 77
CviAII CATG 5 cut(s) 106, 128, 328, 339, 404
CviQI GTAC 2 cut(s) 13, 77
DdeI CTNAG 2 cut(s) 87, 452
DpnI GATC 3 cut(s) 104, 120, 471
DpnII GATC 3 cut(s) 102, 118, 469
Eam1104I CTCTTC 1 cut(s) 250
EarI CTCTTC 1 cut(s) 250
Ecl136II GAGCTC 1 cut(s) 7
Eco24I GRGCYC 2 cut(s) 9, 200
Eco53kI GAGCTC 1 cut(s) 7
Eco57I CTGAAG 1 cut(s) 68
Eco88I CYCGRG 1 cut(s) 377
EcoICRI GAGCTC 1 cut(s) 7
EcoO109I RGGNCCY 1 cut(s) 196
EcoRII CCWGG 2 cut(s) 15, 219
EcoT38I GRGCYC 2 cut(s) 9, 200
FaeI CATG 5 cut(s) 109, 131, 331, 342, 407
FaiI YATR 7 cut(s) 107, 129, 312, 329, 340, 405, 419
FatI CATG 5 cut(s) 105, 127, 327, 338, 403
FbaI TGATCA 1 cut(s) 469
Fnu4HI GCNGC 1 cut(s) 432
FokI GGATG 1 cut(s) 164
FriOI GRGCYC 2 cut(s) 9, 200
Fsp4HI GCNGC 1 cut(s) 432
FspBI CTAG 2 cut(s) 413, 446
GluI GCNGC 1 cut(s) 432
HaeIII GGCC 3 cut(s) 24, 198, 224
Hin1I GRCGYC 1 cut(s) 165
Hin1II CATG 5 cut(s) 109, 131, 331, 342, 407
HinfI GANTC 1 cut(s) 144
HphI GGTGA 2 cut(s) 173, 307
Hpy188I TCNGA 3 cut(s) 48, 143, 248
Hpy188III TCNNGA 2 cut(s) 116, 377
HpyAV CCTTC 3 cut(s) 14, 356, 415
HpyCH4III ACNGT 1 cut(s) 460
HpyCH4IV ACGT 1 cut(s) 165
HpyCH4V TGCA 3 cut(s) 302, 389, 395
HpyF10VI GCNNNNNNNGC 2 cut(s) 422, 431
HpyF3I CTNAG 2 cut(s) 87, 452
HpySE526I ACGT 1 cut(s) 165
Hsp92I GRCGYC 1 cut(s) 165
Hsp92II CATG 5 cut(s) 109, 131, 331, 342, 407
Ksp22I TGATCA 1 cut(s) 469
Kzo9I GATC 3 cut(s) 102, 118, 469
LguI GCTCTTC 1 cut(s) 250
LmnI GCTCC 2 cut(s) 4, 12
LweI GCATC 2 cut(s) 81, 333
MaeI CTAG 2 cut(s) 413, 446
MaeII ACGT 1 cut(s) 165
MaeIII GTNAC 2 cut(s) 161, 330
MalI GATC 3 cut(s) 104, 120, 471
MboI GATC 3 cut(s) 102, 118, 469
MboII GAAGA 1 cut(s) 267
MhlI GDGCHC 2 cut(s) 9, 200
MluCI AATT 2 cut(s) 303, 475
MlyI GAGTC 1 cut(s) 153
MnlI CCTC 3 cut(s) 120, 123, 286
MseI TTAA 2 cut(s) 306, 478
MslI CAYNNNNRTG 1 cut(s) 104
MspR9I CCNGG 2 cut(s) 17, 221
Mva1269I GAATGC 2 cut(s) 239, 304
MvaI CCWGG 2 cut(s) 17, 221
MwoI GCNNNNNNNGC 2 cut(s) 422, 431
NdeII GATC 3 cut(s) 102, 118, 469
NheI GCTAGC 1 cut(s) 412
NlaIII CATG 5 cut(s) 109, 131, 331, 342, 407
NlaIV GGNNCC 1 cut(s) 198
NmuCI GTSAC 1 cut(s) 161
NspI RCATGY 1 cut(s) 342
PaeR7I CTCGAG 1 cut(s) 377
PaqCI CACCTGC 1 cut(s) 400
PciI ACATGT 1 cut(s) 338
PciSI GCTCTTC 1 cut(s) 250
PctI GAATGC 2 cut(s) 239, 304
PflMI CCANNNNNTGG 2 cut(s) 16, 231
PkrI GCNGC 1 cut(s) 433
PleI GAGTC 1 cut(s) 152
PpsI GAGTC 1 cut(s) 152
PscI ACATGT 1 cut(s) 338
PshBI ATTAAT 1 cut(s) 306
Psp124BI GAGCTC 1 cut(s) 9
Psp6I CCWGG 2 cut(s) 15, 219
PspGI CCWGG 2 cut(s) 15, 219
PspN4I GGNNCC 1 cut(s) 198
PspOMI GGGCCC 1 cut(s) 196
PspPI GGNCC 2 cut(s) 196, 197
PstI CTGCAG 1 cut(s) 397
PstNI CAGNNNCTG 1 cut(s) 16
RsaI GTAC 2 cut(s) 14, 78
RsaNI GTAC 2 cut(s) 13, 77
RseI CAYNNNNRTG 1 cut(s) 104
SacI GAGCTC 1 cut(s) 9
SapI GCTCTTC 1 cut(s) 250
SaqAI TTAA 2 cut(s) 306, 478
SatI GCNGC 1 cut(s) 432
Sau3AI GATC 3 cut(s) 102, 118, 469
Sau96I GGNCC 2 cut(s) 196, 197
SchI GAGTC 1 cut(s) 153
ScrFI CCNGG 2 cut(s) 17, 221
SduI GDGCHC 2 cut(s) 9, 200
SetI ASST 8 cut(s) 9, 18, 168, 297, 367, 394, 414, 418
SfaNI GCATC 2 cut(s) 81, 333
SfcI CTRYAG 2 cut(s) 393, 459
Sfr274I CTCGAG 1 cut(s) 377
SlaI CTCGAG 1 cut(s) 377
SmiMI CAYNNNNRTG 1 cut(s) 104
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
Sse9I AATT 2 cut(s) 303, 475
SsiI CCGC 3 cut(s) 322, 431, 441
SspMI CTAG 2 cut(s) 413, 446
SstI GAGCTC 1 cut(s) 9
StyD4I CCNGG 2 cut(s) 15, 219
TaaI ACNGT 1 cut(s) 460
TaiI ACGT 1 cut(s) 168
TaqI TCGA 2 cut(s) 177, 378
TasI AATT 2 cut(s) 303, 475
TauI GCSGC 1 cut(s) 434
Tru1I TTAA 2 cut(s) 306, 478
Tru9I TTAA 2 cut(s) 306, 478
TscAI CASTG 2 cut(s) 36, 463
TseFI GTSAC 1 cut(s) 161
Tsp45I GTSAC 1 cut(s) 161
TspDTI ATGAA 5 cut(s) 232, 360, 414, 420, 434
TspRI CASTG 2 cut(s) 36, 463
Van91I CCANNNNNTGG 2 cut(s) 16, 231
VspI ATTAAT 1 cut(s) 306
XceI RCATGY 1 cut(s) 342
XhoI CTCGAG 1 cut(s) 377
XspI CTAG 2 cut(s) 413, 446
ZraI GACGTC 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.