RLG00000005152

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000707
Physical Location & Seq
Forward (+)
65419459 .. 65419854
396 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005152

Sequence Viewer

Length: 396 bp
ATGGAGCTCGAGTACCTGAGAGGCCATCCAAAGTTTGTGAAAAAGCTGACGCAAACAGATGTGGATGACAGACTTGTAGTGACTAAGGAGATGGGTCTCATGATGCCTGATGCAGATCCAGAACATGACACTTTAATCAGAGTGTTGGACGATGAGGGTGACGACTATCAGTTCTACTTGTCTGTACGAAAGGGCCGAAATCTCAAACCAGTTTTCCAGGCCCAAGGATGGAAAGCATTCATCACAAAACGAGGAGCCAAAGTTGGCGACTTCATCTGTTTCTGGCCAGAGGTGAATGCAATTAAGCGTACAGATTACCGCATCAAGTTATGCCCATTCACATGTTATCTTTCCCCGAATCCAGAAGGTCTTTCATCTTCCAAGCCATCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

132

Amino Acids

15.08

Weight (kDa)

7.68

Isoelectric Point (pI)

22.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 12 - 111 6.1e-07 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 319
AclWI GGATC 1 cut(s) 110
AcoI YGGCCR 1 cut(s) 284
AfaI GTAC 3 cut(s) 14, 186, 310
AfiI CCNNNNNNNGG 1 cut(s) 228
AflIII ACRYGT 1 cut(s) 341
AjnI CCWGG 1 cut(s) 216
AluBI AGCT 2 cut(s) 7, 46
AluI AGCT 2 cut(s) 7, 46
Alw21I GWGCWC 1 cut(s) 9
Alw26I GTCTC 1 cut(s) 101
AlwI GGATC 1 cut(s) 110
Ama87I CYCGRG 1 cut(s) 8
AoxI GGCC 4 cut(s) 22, 193, 219, 284
Asp700I GAANNNNTTC 1 cut(s) 236
AspS9I GGNCC 2 cut(s) 193, 220
AsuHPI GGTGA 2 cut(s) 170, 304
AvaI CYCGRG 1 cut(s) 8
BalI TGGCCA 1 cut(s) 286
BanII GRGCYC 1 cut(s) 9
Bbv12I GWGCWC 1 cut(s) 9
BccI CCATC 4 cut(s) 33, 85, 222, 394
BciT130I CCWGG 1 cut(s) 218
BcoDI GTCTC 1 cut(s) 101
Bme1390I CCNGG 1 cut(s) 218
BmeT110I CYCGRG 1 cut(s) 8
BmgT120I GGNCC 2 cut(s) 193, 220
BmiI GGNNCC 1 cut(s) 256
BmrFI CCNGG 1 cut(s) 218
BmsI GCATC 3 cut(s) 93, 100, 330
BsaBI GATNNNNATC 1 cut(s) 114
BsaI GGTCTC 1 cut(s) 101
BsaJI CCNNGG 1 cut(s) 223
BsaXI ACNNNNNCTCC 3 cut(s) 26, 246, 276
Bsc4I CCNNNNNNNGG 1 cut(s) 228
Bse1I ACTGG 1 cut(s) 209
Bse8I GATNNNNATC 1 cut(s) 114
BseBI CCWGG 1 cut(s) 218
BseDI CCNNGG 1 cut(s) 223
BseGI GGATG 3 cut(s) 25, 70, 233
BseJI GATNNNNATC 1 cut(s) 114
BseLI CCNNNNNNNGG 1 cut(s) 228
BseMII CTCAG 1 cut(s) 8
BseNI ACTGG 1 cut(s) 209
BseRI GAGGAG 1 cut(s) 267
BshFI GGCC 4 cut(s) 24, 195, 221, 286
BsiHKAI GWGCWC 1 cut(s) 9
BsiHKCI CYCGRG 1 cut(s) 8
BslI CCNNNNNNNGG 1 cut(s) 228
BsmAI GTCTC 1 cut(s) 101
BsmI GAATGC 2 cut(s) 236, 301
BsnI GGCC 4 cut(s) 24, 195, 221, 286
Bso31I GGTCTC 1 cut(s) 101
BsoBI CYCGRG 1 cut(s) 8
Bsp1286I GDGCHC 1 cut(s) 9
Bsp143I GATC 1 cut(s) 115
BspACI CCGC 1 cut(s) 319
BspANI GGCC 4 cut(s) 24, 195, 221, 286
BspCNI CTCAG 1 cut(s) 9
BspHI TCATGA 1 cut(s) 99
BspLI GGNNCC 1 cut(s) 256
BspPI GGATC 1 cut(s) 110
BspTNI GGTCTC 1 cut(s) 101
BsrI ACTGG 1 cut(s) 209
BssECI CCNNGG 1 cut(s) 223
BssMI GATC 1 cut(s) 115
BssT1I CCWWGG 1 cut(s) 223
Bst2UI CCWGG 1 cut(s) 218
BstDEI CTNAG 2 cut(s) 17, 84
BstF5I GGATG 3 cut(s) 25, 70, 233
BstKTI GATC 1 cut(s) 118
BstMAI GTCTC 1 cut(s) 101
BstMBI GATC 1 cut(s) 115
BstNI CCWGG 1 cut(s) 218
BstNSI RCATGY 1 cut(s) 345
BstSCI CCNGG 1 cut(s) 216
BstX2I RGATCY 1 cut(s) 115
BstYI RGATCY 1 cut(s) 115
BsuRI GGCC 4 cut(s) 24, 195, 221, 286
BtsCI GGATG 3 cut(s) 25, 70, 233
CciI TCATGA 1 cut(s) 99
Cfr13I GGNCC 2 cut(s) 193, 220
CseI GACGC 1 cut(s) 58
Csp6I GTAC 3 cut(s) 13, 185, 309
CviAII CATG 4 cut(s) 100, 125, 342, 393
CviJI RGCY 8 cut(s) 7, 24, 46, 195, 221, 257, 286, 385
CviKI_1 RGCY 8 cut(s) 7, 24, 46, 195, 221, 257, 286, 385
CviQI GTAC 3 cut(s) 13, 185, 309
DdeI CTNAG 2 cut(s) 17, 84
DpnI GATC 1 cut(s) 117
DpnII GATC 1 cut(s) 115
EaeI YGGCCR 1 cut(s) 284
Ecl136II GAGCTC 1 cut(s) 7
Eco130I CCWWGG 1 cut(s) 223
Eco24I GRGCYC 1 cut(s) 9
Eco31I GGTCTC 1 cut(s) 101
Eco53kI GAGCTC 1 cut(s) 7
Eco88I CYCGRG 1 cut(s) 8
EcoICRI GAGCTC 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 216
EcoT14I CCWWGG 1 cut(s) 223
EcoT38I GRGCYC 1 cut(s) 9
ErhI CCWWGG 1 cut(s) 223
FaeI CATG 4 cut(s) 103, 128, 345, 396
FaiI YATR 5 cut(s) 101, 126, 331, 343, 394
FatI CATG 4 cut(s) 99, 124, 341, 392
FokI GGATG 3 cut(s) 12, 77, 240
FriOI GRGCYC 1 cut(s) 9
HaeIII GGCC 4 cut(s) 24, 195, 221, 286
HgaI GACGC 1 cut(s) 58
Hin1II CATG 4 cut(s) 103, 128, 345, 396
HinfI GANTC 1 cut(s) 358
HphI GGTGA 2 cut(s) 170, 304
Hpy188I TCNGA 1 cut(s) 140
Hpy188III TCNNGA 3 cut(s) 100, 119, 362
HpyAV CCTTC 1 cut(s) 359
HpyCH4V TGCA 3 cut(s) 113, 299, 392
HpyF3I CTNAG 2 cut(s) 17, 84
Hsp92II CATG 4 cut(s) 103, 128, 345, 396
Kzo9I GATC 1 cut(s) 115
LmnI GCTCC 2 cut(s) 4, 254
LpnPI CCDG 9 cut(s) 29, 120, 132, 203, 222, 230, 268, 300, 375
LweI GCATC 3 cut(s) 93, 100, 330
MaeIII GTNAC 2 cut(s) 79, 158
MalI GATC 1 cut(s) 117
MboI GATC 1 cut(s) 115
MboII GAAGA 1 cut(s) 369
MflI RGATCY 1 cut(s) 115
MhlI GDGCHC 1 cut(s) 9
MlsI TGGCCA 1 cut(s) 286
MluCI AATT 1 cut(s) 300
MluNI TGGCCA 1 cut(s) 286
MmeI TCCRAC 1 cut(s) 126
MnlI CCTC 4 cut(s) 14, 148, 245, 283
Mox20I TGGCCA 1 cut(s) 286
MroXI GAANNNNTTC 1 cut(s) 236
MscI TGGCCA 1 cut(s) 286
MseI TTAA 2 cut(s) 134, 303
MslI CAYNNNNRTG 2 cut(s) 340, 391
Msp20I TGGCCA 1 cut(s) 286
MspR9I CCNGG 1 cut(s) 218
Mva1269I GAATGC 2 cut(s) 236, 301
MvaI CCWGG 1 cut(s) 218
NdeII GATC 1 cut(s) 115
NlaIII CATG 4 cut(s) 103, 128, 345, 396
NlaIV GGNNCC 1 cut(s) 256
NmuCI GTSAC 2 cut(s) 79, 158
NspI RCATGY 1 cut(s) 345
PaeR7I CTCGAG 1 cut(s) 8
PagI TCATGA 1 cut(s) 99
PciI ACATGT 1 cut(s) 341
PcsI WCGNNNNNNNCGW 1 cut(s) 193
PctI GAATGC 2 cut(s) 236, 301
PdmI GAANNNNTTC 1 cut(s) 236
PfeI GAWTC 1 cut(s) 358
PscI ACATGT 1 cut(s) 341
Psp124BI GAGCTC 1 cut(s) 9
Psp6I CCWGG 1 cut(s) 216
PspGI CCWGG 1 cut(s) 216
PspN4I GGNNCC 1 cut(s) 256
PspPI GGNCC 2 cut(s) 193, 220
PspXI VCTCGAGB 1 cut(s) 8
PsuI RGATCY 1 cut(s) 115
RsaI GTAC 3 cut(s) 14, 186, 310
RsaNI GTAC 3 cut(s) 13, 185, 309
RseI CAYNNNNRTG 2 cut(s) 340, 391
SacI GAGCTC 1 cut(s) 9
SaqAI TTAA 2 cut(s) 134, 303
Sau3AI GATC 1 cut(s) 115
Sau96I GGNCC 2 cut(s) 193, 220
ScrFI CCNGG 1 cut(s) 218
SduI GDGCHC 1 cut(s) 9
SetI ASST 5 cut(s) 9, 18, 48, 294, 370
SfaNI GCATC 3 cut(s) 93, 100, 330
Sfr274I CTCGAG 1 cut(s) 8
SlaI CTCGAG 1 cut(s) 8
SmiMI CAYNNNNRTG 2 cut(s) 340, 391
SmlI CTYRAG 1 cut(s) 8
SmoI CTYRAG 1 cut(s) 8
Sse9I AATT 1 cut(s) 300
SsiI CCGC 1 cut(s) 319
SstI GAGCTC 1 cut(s) 9
StyD4I CCNGG 1 cut(s) 216
StyI CCWWGG 1 cut(s) 223
TaqI TCGA 1 cut(s) 9
TasI AATT 1 cut(s) 300
TfiI GAWTC 1 cut(s) 358
Tru1I TTAA 2 cut(s) 134, 303
Tru9I TTAA 2 cut(s) 134, 303
TseFI GTSAC 2 cut(s) 79, 158
Tsp45I GTSAC 2 cut(s) 79, 158
TspDTI ATGAA 3 cut(s) 229, 262, 363
XceI RCATGY 1 cut(s) 345
XhoI CTCGAG 1 cut(s) 8
XmnI GAANNNNTTC 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.