RLG00000005157

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000707
Physical Location & Seq
Forward (+)
65523265 .. 65524722
1458 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005157

Sequence Viewer

Length: 666 bp
ATGGATCCATCCTCGTCATCCCAGCTCGATCATTTACTGACAGAGTCCCTAGCTAGTAGCCCTTTCTTAGCAATCTCACATTCCGGAGTCGGCCTCCAAGCAACCCCAAACTCCCTAGTCCTCTCTGTTCGCCCTCAAGAGTTTGTCGATATATCTATCCTGCATACCCTCCCGGATATTTCAGTGCTTCTTACTATCGATCAACCGATGGGGCTCCAGTACCTGAGAGGCCGTCCAATGATTGTGAAACATCTGAAGCAATCAGATGTGGATAGCAGACTTGTAGTGACTACTGAGATGCTTCACATGATCACGCCTCGTCCAGATCCAGAACATGACACTCCAATCAGAGTCTTGGATGATGAGGGTGACGTCTATCAGTTCTACTTGTCTGTAAGAAAGGGCCGACATCCCAAACCAGTTTTCCAGGCCAAAGAAAGGAAAACATTCATCAGAAAACGAGGAGCCAAAAAAAACGACACCATCTATTTCTGGCAAGAGGTGAATGCAATTAAGCGTACAGATTACGGCATCAAGTTATACCCATTGCCATGTTATCTTTTCCCCGAGCCAGAAGGTCTTTCTTCTTCCGAGCCATCTGCACCTGCAGATGAACATGAAAGCCTAGCTATGAGGGCTTGGGAGGCTCGTGGTCTAGCACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

222

Amino Acids

24.94

Weight (kDa)

6.24

Isoelectric Point (pI)

42.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 613
AatII GACGTC 1 cut(s) 375
Acc36I ACCTGC 1 cut(s) 613
AccIII TCCGGA 1 cut(s) 83
AclWI GGATC 2 cut(s) 12, 320
AcuI CTGAAG 1 cut(s) 275
AcyI GRCGYC 1 cut(s) 372
AfaI GTAC 2 cut(s) 221, 520
AfiI CCNNNNNNNGG 1 cut(s) 438
AjnI CCWGG 1 cut(s) 426
AjuI GAANNNNNNNTTGG 2 cut(s) 407, 439
AluBI AGCT 3 cut(s) 25, 53, 629
AluI AGCT 3 cut(s) 25, 53, 629
AlwI GGATC 2 cut(s) 12, 320
AlwNI CAGNNNCTG 1 cut(s) 223
Ama87I CYCGRG 1 cut(s) 566
Aor13HI TCCGGA 1 cut(s) 83
AoxI GGCC 4 cut(s) 91, 229, 403, 429
Asp700I GAANNNNTTC 1 cut(s) 446
AspS9I GGNCC 1 cut(s) 403
AsuC2I CCSGG 1 cut(s) 173
AsuHPI GGTGA 2 cut(s) 380, 514
AvaI CYCGRG 1 cut(s) 566
BamHI GGATCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 216
BauI CACGAG 1 cut(s) 648
BccI CCATC 4 cut(s) 16, 202, 491, 604
BceAI ACGGC 2 cut(s) 216, 544
BcgI CGANNNNNNTGC 2 cut(s) 581, 615
BciT130I CCWGG 1 cut(s) 428
BclI TGATCA 1 cut(s) 309
BcnI CCSGG 1 cut(s) 173
BfaI CTAG 6 cut(s) 50, 54, 116, 626, 656, 664
BfmI CTRYAG 1 cut(s) 606
BfuAI ACCTGC 1 cut(s) 613
Bme1390I CCNGG 2 cut(s) 173, 428
BmeT110I CYCGRG 1 cut(s) 566
BmgT120I GGNCC 1 cut(s) 403
BmiI GGNNCC 3 cut(s) 6, 215, 466
BmrFI CCNGG 2 cut(s) 173, 428
BmsI GCATC 2 cut(s) 288, 540
BplI GAGNNNNNCTC 2 cut(s) 78, 110
BpmI CTGGAG 1 cut(s) 200
BpuEI CTTGAG 1 cut(s) 120
BpuMI CCSGG 1 cut(s) 173
Bsa29I ATCGAT 1 cut(s) 198
BsaHI GRCGYC 1 cut(s) 372
BsaWI WCCGGW 1 cut(s) 83
Bsc4I CCNNNNNNNGG 1 cut(s) 438
Bse1I ACTGG 2 cut(s) 217, 419
Bse3DI GCAATG 1 cut(s) 545
BseAI TCCGGA 1 cut(s) 83
BseBI CCWGG 1 cut(s) 428
BseCI ATCGAT 1 cut(s) 198
BseGI GGATG 4 cut(s) 8, 17, 364, 409
BseLI CCNNNNNNNGG 1 cut(s) 438
BseMI GCAATG 1 cut(s) 545
BseMII CTCAG 2 cut(s) 215, 285
BseNI ACTGG 2 cut(s) 217, 419
BseRI GAGGAG 1 cut(s) 477
BseYI CCCAGC 1 cut(s) 21
BsgI GTGCAG 1 cut(s) 585
BshFI GGCC 4 cut(s) 93, 231, 405, 431
BshVI ATCGAT 1 cut(s) 198
BsiHKCI CYCGRG 1 cut(s) 566
BsiSI CCGG 2 cut(s) 84, 173
BslFI GGGAC 1 cut(s) 31
BslI CCNNNNNNNGG 1 cut(s) 438
BsmFI GGGAC 1 cut(s) 31
BsmI GAATGC 1 cut(s) 511
BsnI GGCC 4 cut(s) 93, 231, 405, 431
BsoBI CYCGRG 1 cut(s) 566
Bsp1286I GDGCHC 1 cut(s) 216
Bsp13I TCCGGA 1 cut(s) 83
Bsp143I GATC 5 cut(s) 4, 28, 199, 309, 325
BspANI GGCC 4 cut(s) 93, 231, 405, 431
BspCNI CTCAG 2 cut(s) 216, 286
BspDI ATCGAT 1 cut(s) 198
BspEI TCCGGA 1 cut(s) 83
BspLI GGNNCC 3 cut(s) 6, 215, 466
BspMAI CTGCAG 1 cut(s) 610
BspMI ACCTGC 1 cut(s) 613
BspPI GGATC 2 cut(s) 12, 320
BsrDI GCAATG 1 cut(s) 545
BsrI ACTGG 2 cut(s) 217, 419
BssMI GATC 5 cut(s) 4, 28, 199, 309, 325
BssNI GRCGYC 1 cut(s) 372
BssSI CACGAG 1 cut(s) 648
Bst2BI CACGAG 1 cut(s) 648
Bst2UI CCWGG 1 cut(s) 428
BstACI GRCGYC 1 cut(s) 372
BstDEI CTNAG 3 cut(s) 67, 224, 294
BstF5I GGATG 4 cut(s) 8, 17, 364, 409
BstKTI GATC 5 cut(s) 7, 31, 202, 312, 328
BstMBI GATC 5 cut(s) 4, 28, 199, 309, 325
BstMWI GCNNNNNNNGC 2 cut(s) 635, 644
BstNI CCWGG 1 cut(s) 428
BstSCI CCNGG 2 cut(s) 171, 426
BstSFI CTRYAG 1 cut(s) 606
BstX2I RGATCY 2 cut(s) 4, 325
BstYI RGATCY 2 cut(s) 4, 325
Bsu15I ATCGAT 1 cut(s) 198
BsuRI GGCC 4 cut(s) 93, 231, 405, 431
BsuTUI ATCGAT 1 cut(s) 198
BtsCI GGATG 4 cut(s) 8, 17, 364, 409
BtsIMutI CAGTG 1 cut(s) 189
BveI ACCTGC 1 cut(s) 613
CaiI CAGNNNCTG 1 cut(s) 223
Cfr13I GGNCC 1 cut(s) 403
ClaI ATCGAT 1 cut(s) 198
Csp6I GTAC 2 cut(s) 220, 519
CviAII CATG 4 cut(s) 307, 335, 552, 617
CviQI GTAC 2 cut(s) 220, 519
DdeI CTNAG 3 cut(s) 67, 224, 294
DpnI GATC 5 cut(s) 6, 30, 201, 311, 327
DpnII GATC 5 cut(s) 4, 28, 199, 309, 325
Eco24I GRGCYC 1 cut(s) 216
Eco57I CTGAAG 1 cut(s) 275
Eco88I CYCGRG 1 cut(s) 566
EcoRII CCWGG 1 cut(s) 426
EcoT38I GRGCYC 1 cut(s) 216
FaeI CATG 4 cut(s) 310, 338, 555, 620
FaiI YATR 8 cut(s) 152, 165, 308, 336, 541, 553, 618, 632
FaqI GGGAC 1 cut(s) 31
FatI CATG 4 cut(s) 306, 334, 551, 616
FbaI TGATCA 1 cut(s) 309
FokI GGATG 3 cut(s) 4, 371, 396
FriOI GRGCYC 1 cut(s) 216
FspBI CTAG 6 cut(s) 50, 54, 116, 626, 656, 664
GsaI CCCAGC 1 cut(s) 25
GsuI CTGGAG 1 cut(s) 200
HaeIII GGCC 4 cut(s) 93, 231, 405, 431
HapII CCGG 2 cut(s) 84, 173
Hin1I GRCGYC 1 cut(s) 372
Hin1II CATG 4 cut(s) 310, 338, 555, 620
HinfI GANTC 3 cut(s) 44, 87, 351
HpaII CCGG 2 cut(s) 84, 173
HphI GGTGA 2 cut(s) 380, 514
Hpy188I TCNGA 5 cut(s) 255, 265, 350, 455, 592
Hpy188III TCNNGA 4 cut(s) 84, 137, 323, 329
HpyAV CCTTC 1 cut(s) 569
HpyCH4IV ACGT 1 cut(s) 372
HpyCH4V TGCA 4 cut(s) 163, 509, 602, 608
HpyF10VI GCNNNNNNNGC 2 cut(s) 635, 644
HpyF3I CTNAG 3 cut(s) 67, 224, 294
HpySE526I ACGT 1 cut(s) 372
Hsp92I GRCGYC 1 cut(s) 372
Hsp92II CATG 4 cut(s) 310, 338, 555, 620
Kpn2I TCCGGA 1 cut(s) 83
Ksp22I TGATCA 1 cut(s) 309
Kzo9I GATC 5 cut(s) 4, 28, 199, 309, 325
LmnI GCTCC 2 cut(s) 219, 464
LweI GCATC 2 cut(s) 288, 540
MaeI CTAG 6 cut(s) 50, 54, 116, 626, 656, 664
MaeII ACGT 1 cut(s) 372
MaeIII GTNAC 2 cut(s) 286, 368
MalI GATC 5 cut(s) 6, 30, 201, 311, 327
MboI GATC 5 cut(s) 4, 28, 199, 309, 325
MboII GAAGA 2 cut(s) 576, 579
MflI RGATCY 2 cut(s) 4, 325
MhlI GDGCHC 1 cut(s) 216
MluCI AATT 1 cut(s) 510
MlyI GAGTC 3 cut(s) 53, 96, 360
MroI TCCGGA 1 cut(s) 83
MroXI GAANNNNTTC 1 cut(s) 446
MseI TTAA 1 cut(s) 513
MslI CAYNNNNRTG 1 cut(s) 550
MspI CCGG 2 cut(s) 84, 173
MspR9I CCNGG 2 cut(s) 173, 428
Mva1269I GAATGC 1 cut(s) 511
MvaI CCWGG 1 cut(s) 428
MwoI GCNNNNNNNGC 2 cut(s) 635, 644
NciI CCSGG 1 cut(s) 173
NdeII GATC 5 cut(s) 4, 28, 199, 309, 325
NlaIII CATG 4 cut(s) 310, 338, 555, 620
NlaIV GGNNCC 3 cut(s) 6, 215, 466
NmuCI GTSAC 2 cut(s) 286, 368
PaqCI CACCTGC 1 cut(s) 613
PctI GAATGC 1 cut(s) 511
PdmI GAANNNNTTC 1 cut(s) 446
PflFI GACNNNGTC 1 cut(s) 43
PfoI TCCNGGA 1 cut(s) 171
PleI GAGTC 3 cut(s) 52, 95, 359
PpsI GAGTC 3 cut(s) 52, 95, 359
Psp6I CCWGG 1 cut(s) 426
PspFI CCCAGC 1 cut(s) 21
PspGI CCWGG 1 cut(s) 426
PspN4I GGNNCC 3 cut(s) 6, 215, 466
PspPI GGNCC 1 cut(s) 403
PstI CTGCAG 1 cut(s) 610
PstNI CAGNNNCTG 1 cut(s) 223
PsuI RGATCY 2 cut(s) 4, 325
PsyI GACNNNGTC 1 cut(s) 43
RsaI GTAC 2 cut(s) 221, 520
RsaNI GTAC 2 cut(s) 220, 519
RseI CAYNNNNRTG 1 cut(s) 550
SaqAI TTAA 1 cut(s) 513
Sau3AI GATC 5 cut(s) 4, 28, 199, 309, 325
Sau96I GGNCC 1 cut(s) 403
SchI GAGTC 3 cut(s) 53, 96, 360
ScrFI CCNGG 2 cut(s) 173, 428
SduI GDGCHC 1 cut(s) 216
SetI ASST 8 cut(s) 27, 55, 225, 375, 504, 580, 607, 631
SfaNI GCATC 2 cut(s) 288, 540
SfcI CTRYAG 1 cut(s) 606
SmiMI CAYNNNNRTG 1 cut(s) 550
SmlI CTYRAG 1 cut(s) 135
SmoI CTYRAG 1 cut(s) 135
Sse9I AATT 1 cut(s) 510
SspMI CTAG 6 cut(s) 50, 54, 116, 626, 656, 664
StyD4I CCNGG 2 cut(s) 171, 426
TaiI ACGT 1 cut(s) 375
TaqI TCGA 3 cut(s) 27, 147, 198
TasI AATT 1 cut(s) 510
Tru1I TTAA 1 cut(s) 513
Tru9I TTAA 1 cut(s) 513
TscAI CASTG 1 cut(s) 189
TseFI GTSAC 2 cut(s) 286, 368
Tsp45I GTSAC 2 cut(s) 286, 368
TspDTI ATGAA 3 cut(s) 439, 627, 633
TspRI CASTG 1 cut(s) 189
Tth111I GACNNNGTC 1 cut(s) 43
XmnI GAANNNNTTC 1 cut(s) 446
XspI CTAG 6 cut(s) 50, 54, 116, 626, 656, 664
ZraI GACGTC 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.