Rmu_ssc0000297.1_g000008

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000297.1
OG0000707
Physical Location & Seq
Reverse (-)
37058 .. 37516
459 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000297.1_g000008.1.cds

Sequence Viewer

Length: 459 bp
atggagcttcagtacctgagaggccgtccaacgtttgtgaaacatctgaagcaatcagatgtggatagcagacttgtagtgactactgagatgcttcacatgatcatgcctcgtccagatccagaacatgacactccaatcagagtcttggatgatgagggtgacgtctatcagttctacttgtctgtgagaaagggccgacatcccaaaccagttttccaggccaaagagtggaaagcattcatcagaaaacgaggagccaaaaaagacgacaccatctatttctggcaagaggtgaatgcaattaagcgtacagattacgtcatcaagttatacccattcccatgttatcttttccccgagccagaagatctttcatcatccgagccatctgcacctgcagatgaacatgaaaacctagctatgagggcttgggcggttcgcggtctagcactctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

152

Amino Acids

17.86

Weight (kDa)

6.97

Isoelectric Point (pI)

41.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 406
AatII GACGTC 1 cut(s) 168
Acc36I ACCTGC 1 cut(s) 406
AccB7I CCANNNNNTGG 1 cut(s) 231
AccII CGCG 1 cut(s) 444
AciI CCGC 2 cut(s) 437, 444
AclI AACGTT 1 cut(s) 32
AclWI GGATC 1 cut(s) 113
AcuI CTGAAG 1 cut(s) 68
AcyI GRCGYC 1 cut(s) 165
AfaI GTAC 2 cut(s) 14, 313
AfiI CCNNNNNNNGG 1 cut(s) 231
AjnI CCWGG 1 cut(s) 219
AjuI GAANNNNNNNTTGG 2 cut(s) 200, 232
AluBI AGCT 2 cut(s) 7, 422
AluI AGCT 2 cut(s) 7, 422
AlwI GGATC 1 cut(s) 113
AlwNI CAGNNNCTG 1 cut(s) 16
Ama87I CYCGRG 1 cut(s) 359
AoxI GGCC 3 cut(s) 22, 196, 222
Asp700I GAANNNNTTC 1 cut(s) 239
AspS9I GGNCC 1 cut(s) 196
AsuHPI GGTGA 2 cut(s) 173, 307
AvaI CYCGRG 1 cut(s) 359
BccI CCATC 2 cut(s) 284, 397
BceAI ACGGC 1 cut(s) 9
BcgI CGANNNNNNTGC 2 cut(s) 374, 408
BciT130I CCWGG 1 cut(s) 221
BclI TGATCA 1 cut(s) 102
BfaI CTAG 3 cut(s) 419, 449, 457
BfmI CTRYAG 1 cut(s) 399
BfuAI ACCTGC 1 cut(s) 406
BglII AGATCT 1 cut(s) 370
Bme1390I CCNGG 1 cut(s) 221
BmeT110I CYCGRG 1 cut(s) 359
BmgT120I GGNCC 1 cut(s) 196
BmiI GGNNCC 1 cut(s) 259
BmrFI CCNGG 1 cut(s) 221
BmsI GCATC 1 cut(s) 81
BsaHI GRCGYC 1 cut(s) 165
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 1 cut(s) 231
Bse1I ACTGG 1 cut(s) 212
BseBI CCWGG 1 cut(s) 221
BseGI GGATG 3 cut(s) 157, 202, 380
BseLI CCNNNNNNNGG 1 cut(s) 231
BseMII CTCAG 2 cut(s) 8, 78
BseNI ACTGG 1 cut(s) 212
BseRI GAGGAG 1 cut(s) 270
BsgI GTGCAG 1 cut(s) 378
Bsh1236I CGCG 1 cut(s) 444
BshFI GGCC 3 cut(s) 24, 198, 224
BsiHKCI CYCGRG 1 cut(s) 359
BslI CCNNNNNNNGG 1 cut(s) 231
BsmI GAATGC 2 cut(s) 239, 304
BsnI GGCC 3 cut(s) 24, 198, 224
BsoBI CYCGRG 1 cut(s) 359
Bsp143I GATC 3 cut(s) 102, 118, 370
BspACI CCGC 2 cut(s) 437, 444
BspANI GGCC 3 cut(s) 24, 198, 224
BspCNI CTCAG 2 cut(s) 9, 79
BspFNI CGCG 1 cut(s) 444
BspLI GGNNCC 1 cut(s) 259
BspMAI CTGCAG 1 cut(s) 403
BspMI ACCTGC 1 cut(s) 406
BspPI GGATC 1 cut(s) 113
BsrI ACTGG 1 cut(s) 212
BssMI GATC 3 cut(s) 102, 118, 370
BssNI GRCGYC 1 cut(s) 165
Bst2UI CCWGG 1 cut(s) 221
BstACI GRCGYC 1 cut(s) 165
BstDEI CTNAG 2 cut(s) 17, 87
BstF5I GGATG 3 cut(s) 157, 202, 380
BstFNI CGCG 1 cut(s) 444
BstKTI GATC 3 cut(s) 105, 121, 373
BstMBI GATC 3 cut(s) 102, 118, 370
BstMWI GCNNNNNNNGC 1 cut(s) 428
BstNI CCWGG 1 cut(s) 221
BstSCI CCNGG 1 cut(s) 219
BstSFI CTRYAG 1 cut(s) 399
BstUI CGCG 1 cut(s) 444
BstX2I RGATCY 2 cut(s) 118, 370
BstYI RGATCY 2 cut(s) 118, 370
BsuRI GGCC 3 cut(s) 24, 198, 224
BtsCI GGATG 3 cut(s) 157, 202, 380
BveI ACCTGC 1 cut(s) 406
CaiI CAGNNNCTG 1 cut(s) 16
Cfr13I GGNCC 1 cut(s) 196
Csp6I GTAC 2 cut(s) 13, 312
CviAII CATG 5 cut(s) 100, 106, 128, 345, 410
CviJI RGCY 9 cut(s) 7, 24, 198, 224, 260, 364, 388, 422, 431
CviKI_1 RGCY 9 cut(s) 7, 24, 198, 224, 260, 364, 388, 422, 431
CviQI GTAC 2 cut(s) 13, 312
DdeI CTNAG 2 cut(s) 17, 87
DpnI GATC 3 cut(s) 104, 120, 372
DpnII GATC 3 cut(s) 102, 118, 370
Eco57I CTGAAG 1 cut(s) 68
Eco88I CYCGRG 1 cut(s) 359
EcoRII CCWGG 1 cut(s) 219
FaeI CATG 5 cut(s) 103, 109, 131, 348, 413
FaiI YATR 7 cut(s) 101, 107, 129, 334, 346, 411, 425
FatI CATG 5 cut(s) 99, 105, 127, 344, 409
FbaI TGATCA 1 cut(s) 102
FokI GGATG 3 cut(s) 164, 189, 367
FspBI CTAG 3 cut(s) 419, 449, 457
HaeIII GGCC 3 cut(s) 24, 198, 224
Hin1I GRCGYC 1 cut(s) 165
Hin1II CATG 5 cut(s) 103, 109, 131, 348, 413
HinfI GANTC 1 cut(s) 144
HphI GGTGA 2 cut(s) 173, 307
Hpy188I TCNGA 5 cut(s) 48, 58, 143, 248, 385
Hpy188III TCNNGA 2 cut(s) 116, 122
HpyCH4IV ACGT 3 cut(s) 32, 165, 321
HpyCH4V TGCA 3 cut(s) 302, 395, 401
HpyF10VI GCNNNNNNNGC 1 cut(s) 428
HpyF3I CTNAG 2 cut(s) 17, 87
HpySE526I ACGT 3 cut(s) 32, 165, 321
Hsp92I GRCGYC 1 cut(s) 165
Hsp92II CATG 5 cut(s) 103, 109, 131, 348, 413
Ksp22I TGATCA 1 cut(s) 102
Kzo9I GATC 3 cut(s) 102, 118, 370
LmnI GCTCC 2 cut(s) 4, 257
LpnPI CCDG 9 cut(s) 29, 129, 135, 206, 225, 233, 271, 378, 411
LweI GCATC 1 cut(s) 81
MaeI CTAG 3 cut(s) 419, 449, 457
MaeII ACGT 3 cut(s) 32, 165, 321
MaeIII GTNAC 2 cut(s) 79, 161
MalI GATC 3 cut(s) 104, 120, 372
MboI GATC 3 cut(s) 102, 118, 370
MboII GAAGA 1 cut(s) 380
MflI RGATCY 2 cut(s) 118, 370
MluCI AATT 1 cut(s) 303
MlyI GAGTC 1 cut(s) 153
MmeI TCCRAC 1 cut(s) 53
MnlI CCTC 6 cut(s) 14, 120, 151, 248, 286, 420
MroXI GAANNNNTTC 1 cut(s) 239
MseI TTAA 1 cut(s) 306
MslI CAYNNNNRTG 2 cut(s) 104, 343
MspR9I CCNGG 1 cut(s) 221
Mva1269I GAATGC 2 cut(s) 239, 304
MvaI CCWGG 1 cut(s) 221
MvnI CGCG 1 cut(s) 444
MwoI GCNNNNNNNGC 1 cut(s) 428
NdeII GATC 3 cut(s) 102, 118, 370
NlaIII CATG 5 cut(s) 103, 109, 131, 348, 413
NlaIV GGNNCC 1 cut(s) 259
NmuCI GTSAC 2 cut(s) 79, 161
PaqCI CACCTGC 1 cut(s) 406
PctI GAATGC 2 cut(s) 239, 304
PdmI GAANNNNTTC 1 cut(s) 239
PflMI CCANNNNNTGG 1 cut(s) 231
PleI GAGTC 1 cut(s) 152
PpsI GAGTC 1 cut(s) 152
Psp1406I AACGTT 1 cut(s) 32
Psp6I CCWGG 1 cut(s) 219
PspGI CCWGG 1 cut(s) 219
PspN4I GGNNCC 1 cut(s) 259
PspPI GGNCC 1 cut(s) 196
PstI CTGCAG 1 cut(s) 403
PstNI CAGNNNCTG 1 cut(s) 16
PsuI RGATCY 2 cut(s) 118, 370
RsaI GTAC 2 cut(s) 14, 313
RsaNI GTAC 2 cut(s) 13, 312
RseI CAYNNNNRTG 2 cut(s) 104, 343
SaqAI TTAA 1 cut(s) 306
Sau3AI GATC 3 cut(s) 102, 118, 370
Sau96I GGNCC 1 cut(s) 196
SchI GAGTC 1 cut(s) 153
ScrFI CCNGG 1 cut(s) 221
SetI ASST 9 cut(s) 9, 18, 35, 168, 297, 324, 400, 420, 424
SfaNI GCATC 1 cut(s) 81
SfcI CTRYAG 1 cut(s) 399
SmiMI CAYNNNNRTG 2 cut(s) 104, 343
Sse9I AATT 1 cut(s) 303
SsiI CCGC 2 cut(s) 437, 444
SspMI CTAG 3 cut(s) 419, 449, 457
StyD4I CCNGG 1 cut(s) 219
TaiI ACGT 3 cut(s) 35, 168, 324
TasI AATT 1 cut(s) 303
Tru1I TTAA 1 cut(s) 306
Tru9I TTAA 1 cut(s) 306
TseFI GTSAC 2 cut(s) 79, 161
Tsp45I GTSAC 2 cut(s) 79, 161
TspDTI ATGAA 4 cut(s) 232, 366, 420, 426
Van91I CCANNNNNTGG 1 cut(s) 231
XmnI GAANNNNTTC 1 cut(s) 239
XspI CTAG 3 cut(s) 419, 449, 457
ZraI GACGTC 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.