Rorug06G0441800

Reverse transcriptase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000707
Physical Location & Seq
Reverse (-)
57395130 .. 57400895
5766 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0441800.1

Sequence Viewer

Length: 2439 bp
ATGTTGGGTTCTTTGGGTCTTCATCTCACTCGTTCTTTTGAGTCTTTATCGTGTTCCAAGGACTACCTGAGTCCGAGGGTAACTCAAACTAAAGCAGTCTCTTTTCAGCTTCGACATAGAGTATTGTCCCCTCCAGGGGGTTTTAGATGTGGGAAGTTGGAGCGTAGTCCCAGTCCCAGAGTTTCAGCAGTTTTGACAGACGACAGCTCGAAAATGACAGTCAGTGATGAAGAGATGGAAGATATTGGTGTTGTGAGAACAGATTCAAACTTGGAGCCATACAAAGATCACTTCAAGCATCGAATAACAAGATATTTGGAGCAGAAGAGTCTCTTTGAGAAATATGAAGGAGGTTTAGAGGAATTCGCGCAAGGTTATGTGAAATTTGGATTTAACAGAGAAGATGGTGGAATTGTGTACCGTGAATGGGCCCCTGCAGCTCAGGAGGCACAACTTGTTGGAGACTTTAATGGATGGGATGGCGGCAAATACGAGATGGAGAAGGGCGAATTTGGGGTATGGAGTATTAAGATACCCGATTTTGATGGAAGTCCAGCTATTCCTCACAATTCAAAGGTTAAATTCCGGTTCAAGCATGGAAATGGAGTTTGGGTTGATCGTATTCCTGCTTGGATTAAATATGCCACTGTGGACCCAACAAGATTTGCAGCTCCATATGATGGTGTGTACTGGGATCCACCACCATCAGAAAGGTATCAGTTTAAGTACGGTCGTCCTCCAAAACCCAAGGCTCCACGAATATATGAGGCCCATGTTGGAATGAGTAGCTCAGAACCCCGCATCAGTTCATACAGAGAATTTGCTGATAATGTTTTGCCTCGTATCCAAGCAAATAACTATAACACAGTCCAGCTGATGGCCGTGATGGAGCATTCCTATTATGCATCCTTCGGTTATCATGTGACAAACTTCTTTGCCGTAAGCAGTAGATCTGGAACACCTGAGGACCTTAAATATCTAATTGATAAAGCACATTCCCTAGGTTTGCGTGTATTGATGGATGTTGTTCACAGTCATGCAAGTAATAACGTCACTGATGGCCTCAGTGGCTTTGAAGTTGGTCAAAGCACACAAGATTCCTATTTCCACACAGGAGATAGAGGCTACCATAAGCTATGGGATAGCCGACTTTTCAACTATGCTAATTGGGAAGTTCTCCGCTTCTTTTTATCCAACTTAAGGTGGTGGCTGGAGGAGTATCAATTTGATGGGTTTCGATTTGATGGAGTAACTTCCATGCTGTATCATCACCATGGCATAAATATGGCATTTACAGGGAATTATGACGAGTACTTCAGTGAGGCAACAGATGTTGATGCTGTTGTTTATTTGATGCTTGCCAACTATCTGATCCATAAAATATTGCCATCTGCAACTGTAATTGCTGAAGATGTTTCTGGGATGCCTGGACTTGGTCGGCCTGTCTCTGAAGGAGGTGTTGGTTTTGACTACCGGCTGGCAATGGCCATCCCTGACAAATGGATTGATTATGTGAAAAACAAGAAAGACGAAGAGTGGTCGATGAAGGAAATTTCATGGAGCTTGACGAATAGGAGATACACTGAGAAGTGTGTTTCCTATGCTGAGAGTCATGACCAGGCAATTGTGGGTGACAAGACGATTGCATTCTTTTTGATGGATAAAGAAATGTACTCTGGCATGTCTTGTTTGAATGAAGCATCTCCTACTGTTGAGCGCGGGGTTGCTCTTCACAAGATGATACATTTTATAACTATGGCATTAGGAGGCGAGGGTTACCTTAACTTTATGGGTAATGAGTTTGGACACCCTGAGTGGATCGACTTTCCAAGAGAAGGCAATGAGTGGAGTTATGAAAAGTGCAGACGCCAATGGAACCTGGTGGATACAGATCATTTAAGATATAAGTTTATGAACGCATTTGACAGAGCTATGCACTTGCTTGATGAGAAATTTTCGTTCCTTTCATCAACGAAGCAGATAGTGAGCAGCACAAATGAAGAAGACAAGGTTATTGTCTTTGAGCGTGGAGATCTAGTTTTTGTATTTAATTTTCATCCAGTAAACACATACGATGGGTACAAAGTGGGGTGTGACTTACCTGGGAAGTACAGAGTTGCACTAGATAGTGATGCTTTTGAATTCGGTGGACCTGGAAGAGTAGGCCATGATGTAGACCACTTCACATTCCCAGAAGGAATACCAGGGGTTCCTGAAACAAATTTCAACGGCCGTCCCAACTCCTTAAGAGTACTCTCACCACCGCGAACATGCGTGGTTTATTATCGAGTGGATGAAAGTAAGGAGACTGCTGTGAAGGAGGACTCGGCAGCAGAAGTTGTGGTTGGGCGGGAGAACTCCCAATTGCTTGGATCCGTGAAAGATGGAAATGTTGGTCCAGGAGTGGAAGAGACCGTCAAAGTTTCGTCAGATAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

812

Amino Acids

92.44

Weight (kDa)

5.62

Isoelectric Point (pI)

33.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBM_48 PF02922 128 - 211 4.6e-20 Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase PF00128 301 - 353 3.4e-09 Alpha amylase, catalytic domain
Alpha-amylase_C PF02806 663 - 763 5e-20 Alpha amylase, C-terminal all-beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1751
AccB7I CCANNNNNTGG 2 cut(s) 680, 877
AccI GTMKAC 1 cut(s) 2175
AccII CGCG 3 cut(s) 368, 1719, 2266
AciI CCGC 6 cut(s) 483, 799, 1180, 1719, 2264, 2350
AclWI GGATC 6 cut(s) 689, 702, 1366, 1826, 2367, 2380
AcoI YGGCCR 3 cut(s) 879, 1485, 2230
AcsI RAATTY 9 cut(s) 362, 383, 509, 581, 818, 1551, 1952, 2141, 2221
AcuI CTGAAG 3 cut(s) 1300, 1428, 1470
AcyI GRCGYC 1 cut(s) 1867
AfaI GTAC 8 cut(s) 419, 689, 728, 1313, 1673, 2081, 2111, 2253
AfiI CCNNNNNNNGG 9 cut(s) 135, 136, 137, 427, 680, 877, 1200, 1433, 1835
AflII CTTAAG 2 cut(s) 1198, 2245
AgsI TTSAA 9 cut(s) 267, 295, 573, 592, 1076, 1156, 1693, 2141, 2227
AjnI CCWGG 8 cut(s) 133, 1426, 1617, 1878, 2101, 2152, 2203, 2398
AjuI GAANNNNNNNTTGG 6 cut(s) 592, 624, 1443, 1475, 2328, 2360
Alw26I GTCTC 6 cut(s) 103, 335, 456, 1450, 2300, 2405
AlwI GGATC 6 cut(s) 689, 702, 1366, 1826, 2367, 2380
AoxI GGCC 8 cut(s) 429, 768, 879, 1060, 1439, 1485, 2164, 2230
ApaI GGGCCC 1 cut(s) 433
ApeKI GCWGC 4 cut(s) 437, 668, 1989, 2330
ApoI RAATTY 9 cut(s) 362, 383, 509, 581, 818, 1551, 1952, 2141, 2221
Asp700I GAANNNNTTC 1 cut(s) 262
AspA2I CCTAGG 1 cut(s) 1000
AspLEI GCGC 2 cut(s) 370, 1719
AspS9I GGNCC 7 cut(s) 429, 430, 652, 769, 967, 2150, 2396
AsuHPI GGTGA 3 cut(s) 1262, 1643, 2250
AvaII GGWCC 4 cut(s) 652, 967, 2150, 2396
AvrII CCTAGG 1 cut(s) 1000
AxyI CCTNAGG 1 cut(s) 963
BaeGI GKGCMC 1 cut(s) 433
BalI TGGCCA 1 cut(s) 1487
BamHI GGATCC 2 cut(s) 694, 2372
BanII GRGCYC 1 cut(s) 433
BbsI GAAGAC 2 cut(s) 11, 2010
BbvI GCAGC 4 cut(s) 449, 680, 2001, 2342
BceAI ACGGC 4 cut(s) 866, 923, 2217, 2245
BciT130I CCWGG 8 cut(s) 135, 1428, 1619, 1880, 2103, 2154, 2205, 2400
BciVI GTATCC 2 cut(s) 854, 1879
BcoDI GTCTC 6 cut(s) 103, 335, 456, 1450, 2300, 2405
BfaI CTAG 3 cut(s) 1001, 2036, 2123
BfmI CTRYAG 1 cut(s) 435
BfrI CTTAAG 2 cut(s) 1198, 2245
BfuI GTATCC 2 cut(s) 854, 1879
BglI GCCNNNNNGGC 1 cut(s) 1068
BglII AGATCT 2 cut(s) 950, 2032
BisI GCNGC 5 cut(s) 438, 484, 669, 1990, 2331
BlnI CCTAGG 1 cut(s) 1000
BlsI GCNGC 5 cut(s) 439, 485, 670, 1991, 2332
BmcAI AGTACT 2 cut(s) 1313, 2253
Bme1390I CCNGG 8 cut(s) 135, 1428, 1619, 1880, 2103, 2154, 2205, 2400
Bme18I GGWCC 4 cut(s) 652, 967, 2150, 2396
BmgT120I GGNCC 7 cut(s) 429, 430, 652, 769, 967, 2150, 2396
BmiI GGNNCC 9 cut(s) 276, 431, 432, 654, 696, 753, 1877, 2211, 2374
BmrFI CCNGG 8 cut(s) 135, 1428, 1619, 1880, 2103, 2154, 2205, 2400
BmrI ACTGGG 2 cut(s) 165, 700
BmsI GCATC 8 cut(s) 307, 810, 914, 1327, 1344, 1413, 1709, 2122
BmuI ACTGGG 2 cut(s) 165, 700
BpiI GAAGAC 2 cut(s) 11, 2010
BplI GAGNNNNNCTC 2 cut(s) 67, 99
BpmI CTGGAG 2 cut(s) 117, 1232
Bpu10I CCTNAGC 1 cut(s) 441
BsaBI GATNNNNATC 1 cut(s) 1890
BsaHI GRCGYC 1 cut(s) 1867
BsaI GGTCTC 1 cut(s) 2405
BsaJI CCNNGG 8 cut(s) 57, 74, 134, 747, 1000, 1273, 2102, 2204
BsaWI WCCGGW 1 cut(s) 585
BsaXI ACNNNNNCTCC 4 cut(s) 597, 627, 2022, 2052
Bsc4I CCNNNNNNNGG 9 cut(s) 135, 136, 137, 427, 680, 877, 1200, 1433, 1835
Bse118I RCCGGY 1 cut(s) 1473
Bse1I ACTGG 3 cut(s) 171, 695, 2060
Bse21I CCTNAGG 1 cut(s) 963
Bse3DI GCAATG 2 cut(s) 1488, 1846
Bse8I GATNNNNATC 1 cut(s) 1890
BseBI CCWGG 8 cut(s) 135, 1428, 1619, 1880, 2103, 2154, 2205, 2400
BseDI CCNNGG 8 cut(s) 57, 74, 134, 747, 1000, 1273, 2102, 2204
BseGI GGATG 8 cut(s) 479, 484, 905, 1027, 1428, 1488, 2056, 2299
BseJI GATNNNNATC 1 cut(s) 1890
BseLI CCNNNNNNNGG 9 cut(s) 135, 136, 137, 427, 680, 877, 1200, 1433, 1835
BseMI GCAATG 2 cut(s) 1488, 1846
BseMII CTCAG 8 cut(s) 59, 455, 804, 954, 1078, 1575, 1596, 1803
BseNI ACTGG 3 cut(s) 171, 695, 2060
BseRI GAGGAG 1 cut(s) 1229
BseSI GKGCMC 1 cut(s) 433
BseX3I CGGCCG 1 cut(s) 2230
BseXI GCAGC 4 cut(s) 449, 680, 2001, 2342
BsgI GTGCAG 1 cut(s) 1882
Bsh1236I CGCG 3 cut(s) 368, 1719, 2266
Bsh1285I CGRYCG 2 cut(s) 733, 2233
BshFI GGCC 8 cut(s) 431, 770, 881, 1062, 1441, 1487, 2166, 2232
BsiEI CGRYCG 2 cut(s) 733, 2233
BsiSI CCGG 2 cut(s) 586, 1474
BslFI GGGAC 4 cut(s) 112, 153, 159, 2220
BslI CCNNNNNNNGG 9 cut(s) 135, 136, 137, 427, 680, 877, 1200, 1433, 1835
BsmAI GTCTC 6 cut(s) 103, 335, 456, 1450, 2300, 2405
BsmFI GGGAC 4 cut(s) 112, 153, 159, 2220
BsmI GAATGC 2 cut(s) 892, 1646
BsnI GGCC 8 cut(s) 431, 770, 881, 1062, 1441, 1487, 2166, 2232
Bso31I GGTCTC 1 cut(s) 2405
Bsp120I GGGCCC 1 cut(s) 429
Bsp1286I GDGCHC 1 cut(s) 433
Bsp143I GATC 9 cut(s) 286, 616, 694, 950, 1371, 1818, 1891, 2032, 2372
Bsp19I CCATGG 1 cut(s) 1273
BspACI CCGC 6 cut(s) 483, 799, 1180, 1719, 2264, 2350
BspANI GGCC 8 cut(s) 431, 770, 881, 1062, 1441, 1487, 2166, 2232
BspCNI CTCAG 8 cut(s) 60, 454, 803, 955, 1077, 1576, 1597, 1804
BspFNI CGCG 3 cut(s) 368, 1719, 2266
BspHI TCATGA 1 cut(s) 1612
BspLI GGNNCC 9 cut(s) 276, 431, 432, 654, 696, 753, 1877, 2211, 2374
BspMAI CTGCAG 1 cut(s) 439
BspPI GGATC 6 cut(s) 689, 702, 1366, 1826, 2367, 2380
BspQI GCTCTTC 1 cut(s) 1734
BspTI CTTAAG 2 cut(s) 1198, 2245
BspTNI GGTCTC 1 cut(s) 2405
BsrDI GCAATG 2 cut(s) 1488, 1846
BsrFI RCCGGY 1 cut(s) 1473
BsrI ACTGG 3 cut(s) 171, 695, 2060
BssAI RCCGGY 1 cut(s) 1473
BssECI CCNNGG 8 cut(s) 57, 74, 134, 747, 1000, 1273, 2102, 2204
BssMI GATC 9 cut(s) 286, 616, 694, 950, 1371, 1818, 1891, 2032, 2372
BssNI GRCGYC 1 cut(s) 1867
BssT1I CCWWGG 4 cut(s) 57, 747, 1000, 1273
Bst2UI CCWGG 8 cut(s) 135, 1428, 1619, 1880, 2103, 2154, 2205, 2400
Bst4CI ACNGT 9 cut(s) 220, 422, 649, 731, 868, 1034, 1399, 1711, 2416
Bst6I CTCTTC 6 cut(s) 225, 320, 1527, 1734, 2152, 2403
BstACI GRCGYC 1 cut(s) 1867
BstAFI CTTAAG 2 cut(s) 1198, 2245
BstC8I GCNNGC 2 cut(s) 1359, 1479
BstDEI CTNAG 8 cut(s) 68, 441, 790, 963, 1064, 1584, 1605, 1812
BstDSI CCRYGG 1 cut(s) 1273
BstEII GGTNACC 1 cut(s) 1775
BstF5I GGATG 8 cut(s) 479, 484, 905, 1027, 1428, 1488, 2056, 2299
BstFNI CGCG 3 cut(s) 368, 1719, 2266
BstHHI GCGC 2 cut(s) 370, 1719
BstKTI GATC 9 cut(s) 289, 619, 697, 953, 1374, 1821, 1894, 2035, 2375
BstMAI GTCTC 6 cut(s) 103, 335, 456, 1450, 2300, 2405
BstMBI GATC 9 cut(s) 286, 616, 694, 950, 1371, 1818, 1891, 2032, 2372
BstMCI CGRYCG 2 cut(s) 733, 2233
BstMWI GCNNNNNNNGC 3 cut(s) 437, 446, 1068
BstNI CCWGG 8 cut(s) 135, 1428, 1619, 1880, 2103, 2154, 2205, 2400
BstNSI RCATGY 2 cut(s) 1684, 2274
BstPI GGTNACC 1 cut(s) 1775
BstSCI CCNGG 8 cut(s) 133, 1426, 1617, 1878, 2101, 2152, 2203, 2398
BstSFI CTRYAG 1 cut(s) 435
BstSLI GKGCMC 1 cut(s) 433
BstUI CGCG 3 cut(s) 368, 1719, 2266
BstV1I GCAGC 4 cut(s) 449, 680, 2001, 2342
BstV2I GAAGAC 2 cut(s) 11, 2010
BstX2I RGATCY 4 cut(s) 694, 950, 2032, 2372
BstXI CCANNNNNNTGG 1 cut(s) 2369
BstYI RGATCY 4 cut(s) 694, 950, 2032, 2372
BstZI CGGCCG 1 cut(s) 2230
Bsu36I CCTNAGG 1 cut(s) 963
BsuI GTATCC 2 cut(s) 854, 1879
BsuRI GGCC 8 cut(s) 431, 770, 881, 1062, 1441, 1487, 2166, 2232
BtgI CCRYGG 1 cut(s) 1273
BtsCI GGATG 8 cut(s) 479, 484, 905, 1027, 1428, 1488, 2056, 2299
BtsIMutI CAGTG 6 cut(s) 229, 645, 1053, 1072, 1324, 1581
Cac8I GCNNGC 2 cut(s) 1359, 1479
CciI TCATGA 1 cut(s) 1612
CfoI GCGC 2 cut(s) 370, 1719
Cfr10I RCCGGY 1 cut(s) 1473
Cfr13I GGNCC 7 cut(s) 429, 430, 652, 769, 967, 2150, 2396
CseI GACGC 1 cut(s) 1875
CsiI ACCWGGT 1 cut(s) 1878
Csp6I GTAC 8 cut(s) 418, 688, 727, 1312, 1672, 2080, 2110, 2252
CviQI GTAC 8 cut(s) 418, 688, 727, 1312, 1672, 2080, 2110, 2252
DdeI CTNAG 8 cut(s) 68, 441, 790, 963, 1064, 1584, 1605, 1812
DpnI GATC 9 cut(s) 288, 618, 696, 952, 1373, 1820, 1893, 2034, 2374
DpnII GATC 9 cut(s) 286, 616, 694, 950, 1371, 1818, 1891, 2032, 2372
EaeI YGGCCR 3 cut(s) 879, 1485, 2230
EagI CGGCCG 1 cut(s) 2230
Eam1104I CTCTTC 6 cut(s) 225, 320, 1527, 1734, 2152, 2403
EarI CTCTTC 6 cut(s) 225, 320, 1527, 1734, 2152, 2403
EclXI CGGCCG 1 cut(s) 2230
Eco130I CCWWGG 4 cut(s) 57, 747, 1000, 1273
Eco24I GRGCYC 1 cut(s) 433
Eco31I GGTCTC 1 cut(s) 2405
Eco47I GGWCC 4 cut(s) 652, 967, 2150, 2396
Eco52I CGGCCG 1 cut(s) 2230
Eco57I CTGAAG 3 cut(s) 1300, 1428, 1470
Eco81I CCTNAGG 1 cut(s) 963
Eco91I GGTNACC 1 cut(s) 1775
EcoO109I RGGNCCY 2 cut(s) 430, 967
EcoO65I GGTNACC 1 cut(s) 1775
EcoRI GAATTC 2 cut(s) 362, 2141
EcoRII CCWGG 8 cut(s) 133, 1426, 1617, 1878, 2101, 2152, 2203, 2398
EcoT14I CCWWGG 4 cut(s) 57, 747, 1000, 1273
EcoT22I ATGCAT 1 cut(s) 907
EcoT38I GRGCYC 1 cut(s) 433
ErhI CCWWGG 4 cut(s) 57, 747, 1000, 1273
FalI AAGNNNNNCTT 2 cut(s) 317, 349
FaqI GGGAC 4 cut(s) 112, 153, 159, 2220
FauI CCCGC 3 cut(s) 806, 1712, 2343
FauNDI CATATG 1 cut(s) 676
FblI GTMKAC 1 cut(s) 2175
Fnu4HI GCNGC 5 cut(s) 438, 484, 669, 1990, 2331
FokI GGATG 8 cut(s) 486, 491, 892, 1034, 1435, 1475, 2043, 2306
FriOI GRGCYC 1 cut(s) 433
Fsp4HI GCNGC 5 cut(s) 438, 484, 669, 1990, 2331
FspBI CTAG 3 cut(s) 1001, 2036, 2123
GlaI GCGC 2 cut(s) 369, 1718
GluI GCNGC 5 cut(s) 438, 484, 669, 1990, 2331
GsuI CTGGAG 2 cut(s) 117, 1232
HaeIII GGCC 8 cut(s) 431, 770, 881, 1062, 1441, 1487, 2166, 2232
HapII CCGG 2 cut(s) 586, 1474
HgaI GACGC 1 cut(s) 1875
HhaI GCGC 2 cut(s) 370, 1719
Hin1I GRCGYC 1 cut(s) 1867
Hin6I GCGC 2 cut(s) 368, 1717
HinP1I GCGC 2 cut(s) 368, 1717
HinfI GANTC 7 cut(s) 41, 70, 263, 328, 1097, 1609, 2324
HpaII CCGG 2 cut(s) 586, 1474
HphI GGTGA 3 cut(s) 1262, 1643, 2250
Hpy166II GTNNAC 7 cut(s) 418, 652, 688, 1030, 2065, 2150, 2176
Hpy188I TCNGA 6 cut(s) 75, 709, 793, 1371, 1450, 2431
Hpy188III TCNNGA 4 cut(s) 443, 954, 1613, 2213
Hpy8I GTNNAC 7 cut(s) 418, 652, 688, 1030, 2065, 2150, 2176
HpyAV CCTTC 8 cut(s) 341, 496, 919, 1445, 1540, 1829, 2189, 2311
HpyCH4III ACNGT 9 cut(s) 220, 422, 649, 731, 868, 1034, 1399, 1711, 2416
HpyCH4IV ACGT 1 cut(s) 1050
HpyCH4V TGCA 9 cut(s) 437, 668, 905, 1040, 1394, 1646, 1863, 1936, 2120
HpyF10VI GCNNNNNNNGC 3 cut(s) 437, 446, 1068
HpyF3I CTNAG 8 cut(s) 68, 441, 790, 963, 1064, 1584, 1605, 1812
HpySE526I ACGT 1 cut(s) 1050
Hsp92I GRCGYC 1 cut(s) 1867
HspAI GCGC 2 cut(s) 368, 1717
Kzo9I GATC 9 cut(s) 286, 616, 694, 950, 1371, 1818, 1891, 2032, 2372
LguI GCTCTTC 1 cut(s) 1734
LmnI GCTCC 7 cut(s) 160, 274, 319, 676, 757, 889, 1560
Lsp1109I GCAGC 4 cut(s) 449, 680, 2001, 2342
LweI GCATC 8 cut(s) 307, 810, 914, 1327, 1344, 1413, 1709, 2122
MabI ACCWGGT 1 cut(s) 1878
MaeI CTAG 3 cut(s) 1001, 2036, 2123
MaeII ACGT 1 cut(s) 1050
MaeIII GTNAC 7 cut(s) 79, 922, 1051, 1249, 1631, 1775, 2093
MalI GATC 9 cut(s) 288, 618, 696, 952, 1373, 1820, 1893, 2034, 2374
MboI GATC 9 cut(s) 286, 616, 694, 950, 1371, 1818, 1891, 2032, 2372
MfeI CAATTG 2 cut(s) 1623, 2363
MflI RGATCY 4 cut(s) 694, 950, 2032, 2372
MhlI GDGCHC 1 cut(s) 433
MlsI TGGCCA 1 cut(s) 1487
MluNI TGGCCA 1 cut(s) 1487
MlyI GAGTC 5 cut(s) 50, 79, 337, 1618, 2318
MmeI TCCRAC 4 cut(s) 138, 439, 757, 1218
Mox20I TGGCCA 1 cut(s) 1487
Mph1103I ATGCAT 1 cut(s) 907
MroXI GAANNNNTTC 1 cut(s) 262
MscI TGGCCA 1 cut(s) 1487
MslI CAYNNNNRTG 4 cut(s) 600, 1035, 1272, 1283
Msp20I TGGCCA 1 cut(s) 1487
MspA1I CMGCKG 1 cut(s) 874
MspCI CTTAAG 2 cut(s) 1198, 2245
MspI CCGG 2 cut(s) 586, 1474
MspR9I CCNGG 8 cut(s) 135, 1428, 1619, 1880, 2103, 2154, 2205, 2400
MunI CAATTG 2 cut(s) 1623, 2363
Mva1269I GAATGC 2 cut(s) 892, 1646
MvaI CCWGG 8 cut(s) 135, 1428, 1619, 1880, 2103, 2154, 2205, 2400
MvnI CGCG 3 cut(s) 368, 1719, 2266
MwoI GCNNNNNNNGC 3 cut(s) 437, 446, 1068
NcoI CCATGG 1 cut(s) 1273
NdeI CATATG 1 cut(s) 676
NdeII GATC 9 cut(s) 286, 616, 694, 950, 1371, 1818, 1891, 2032, 2372
NlaIV GGNNCC 9 cut(s) 276, 431, 432, 654, 696, 753, 1877, 2211, 2374
NmeAIII GCCGAG 1 cut(s) 2306
NmuCI GTSAC 4 cut(s) 922, 1051, 1631, 2093
NsiI ATGCAT 1 cut(s) 907
NspI RCATGY 2 cut(s) 1684, 2274
PagI TCATGA 1 cut(s) 1612
PciSI GCTCTTC 1 cut(s) 1734
PctI GAATGC 2 cut(s) 892, 1646
PdmI GAANNNNTTC 1 cut(s) 262
PfeI GAWTC 2 cut(s) 263, 1097
PflFI GACNNNGTC 1 cut(s) 1434
PflMI CCANNNNNTGG 2 cut(s) 680, 877
PfoI TCCNGGA 1 cut(s) 2398
PkrI GCNGC 5 cut(s) 439, 485, 670, 1991, 2332
PleI GAGTC 5 cut(s) 49, 78, 336, 1617, 2318
PpsI GAGTC 5 cut(s) 49, 78, 336, 1617, 2318
PpuMI RGGWCCY 1 cut(s) 967
PsiI TTATAA 1 cut(s) 1751
Psp5II RGGWCCY 1 cut(s) 967
Psp6I CCWGG 8 cut(s) 133, 1426, 1617, 1878, 2101, 2152, 2203, 2398
PspEI GGTNACC 1 cut(s) 1775
PspGI CCWGG 8 cut(s) 133, 1426, 1617, 1878, 2101, 2152, 2203, 2398
PspN4I GGNNCC 9 cut(s) 276, 431, 432, 654, 696, 753, 1877, 2211, 2374
PspOMI GGGCCC 1 cut(s) 429
PspPI GGNCC 7 cut(s) 429, 430, 652, 769, 967, 2150, 2396
PspPPI RGGWCCY 1 cut(s) 967
PstI CTGCAG 1 cut(s) 439
PsuI RGATCY 4 cut(s) 694, 950, 2032, 2372
PsyI GACNNNGTC 1 cut(s) 1434
PvuII CAGCTG 1 cut(s) 874
RsaI GTAC 8 cut(s) 419, 689, 728, 1313, 1673, 2081, 2111, 2253
RsaNI GTAC 8 cut(s) 418, 688, 727, 1312, 1672, 2080, 2110, 2252
RseI CAYNNNNRTG 4 cut(s) 600, 1035, 1272, 1283
SapI GCTCTTC 1 cut(s) 1734
SatI GCNGC 5 cut(s) 438, 484, 669, 1990, 2331
Sau3AI GATC 9 cut(s) 286, 616, 694, 950, 1371, 1818, 1891, 2032, 2372
Sau96I GGNCC 7 cut(s) 429, 430, 652, 769, 967, 2150, 2396
ScaI AGTACT 2 cut(s) 1313, 2253
SchI GAGTC 5 cut(s) 50, 79, 337, 1618, 2318
ScrFI CCNGG 8 cut(s) 135, 1428, 1619, 1880, 2103, 2154, 2205, 2400
SduI GDGCHC 1 cut(s) 433
SexAI ACCWGGT 1 cut(s) 1878
SfaNI GCATC 8 cut(s) 307, 810, 914, 1327, 1344, 1413, 1709, 2122
SfcI CTRYAG 1 cut(s) 435
SinI GGWCC 4 cut(s) 652, 967, 2150, 2396
SmiMI CAYNNNNRTG 4 cut(s) 600, 1035, 1272, 1283
SmlI CTYRAG 2 cut(s) 1198, 2245
SmoI CTYRAG 2 cut(s) 1198, 2245
SsiI CCGC 6 cut(s) 483, 799, 1180, 1719, 2264, 2350
SspI AATATT 1 cut(s) 1383
SspMI CTAG 3 cut(s) 1001, 2036, 2123
StyD4I CCNGG 8 cut(s) 133, 1426, 1617, 1878, 2101, 2152, 2203, 2398
StyI CCWWGG 4 cut(s) 57, 747, 1000, 1273
TaaI ACNGT 9 cut(s) 220, 422, 649, 731, 868, 1034, 1399, 1711, 2416
TaiI ACGT 1 cut(s) 1053
TaqI TCGA 7 cut(s) 112, 209, 301, 1237, 1541, 1821, 2287
TatI WGTACW 5 cut(s) 687, 1311, 1671, 2109, 2251
TauI GCSGC 1 cut(s) 486
TfiI GAWTC 2 cut(s) 263, 1097
TscAI CASTG 6 cut(s) 229, 652, 1060, 1072, 1324, 1588
TseFI GTSAC 4 cut(s) 922, 1051, 1631, 2093
TseI GCWGC 4 cut(s) 437, 668, 1989, 2330
Tsp45I GTSAC 4 cut(s) 922, 1051, 1631, 2093
TspGWI ACGGA 1 cut(s) 2365
TspRI CASTG 6 cut(s) 229, 652, 1060, 1072, 1324, 1588
Tth111I GACNNNGTC 1 cut(s) 1434
Van91I CCANNNNNTGG 2 cut(s) 680, 877
Vha464I CTTAAG 2 cut(s) 1198, 2245
VpaK11BI GGWCC 4 cut(s) 652, 967, 2150, 2396
XapI RAATTY 9 cut(s) 362, 383, 509, 581, 818, 1551, 1952, 2141, 2221
XceI RCATGY 2 cut(s) 1684, 2274
XmaJI CCTAGG 1 cut(s) 1000
XmiI GTMKAC 1 cut(s) 2175
XmnI GAANNNNTTC 1 cut(s) 262
XspI CTAG 3 cut(s) 1001, 2036, 2123
ZrmI AGTACT 2 cut(s) 1313, 2253
Zsp2I ATGCAT 1 cut(s) 907
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.