Rh7DG047000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000707
Physical Location & Seq
Reverse (-)
3615371 .. 3615820
450 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG047000.1

Sequence Viewer

Length: 450 bp
ATGGAGCTCGAGTACCTGAAATGCCTTCCAAAGTTTGTGAAAGAGCTTAAGCCGACAGATCTGGATAGCAGACTTGTACTGAGTAATGAGATCCTTCTCATGGTACCTCATGCAGGTCCAGAACGTGACACTCCAATCAGAGTGTTGGACGATGAGGGTGACGACTATCTGTTCTGCTTGTCTCTAAGAAAGGGCCCACATCTCAAACCAGTTTTCCAGGCCAAAGGATGGAAAACATTCATCAAAAAACGAGGAGCCAAAATTGGCGACAAAATCTGTTTCTGGCCGGAGGTGAATGCAATTAAGCGTACAGATTACCGCATCCAGTTAATTCCATTCTCACCTTATCTTTCCCCCAACCCAGAAGGTCTTTCATCTTCCAGGCCATCTGCACCTGCAGAGGAACATGAAACCCTAGCGATTTGGGCGGCTAGCGGACTAGCACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

149

Amino Acids

16.85

Weight (kDa)

7.7

Isoelectric Point (pI)

38.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 403
Acc36I ACCTGC 2 cut(s) 104, 403
Acc65I GGTACC 1 cut(s) 103
AccB1I GGYRCC 1 cut(s) 103
AccB7I CCANNNNNTGG 1 cut(s) 228
AciI CCGC 3 cut(s) 319, 428, 435
AclWI GGATC 1 cut(s) 85
AcoI YGGCCR 1 cut(s) 284
AfaI GTAC 4 cut(s) 14, 78, 105, 310
AfiI CCNNNNNNNGG 3 cut(s) 100, 113, 228
AflII CTTAAG 1 cut(s) 47
AjnI CCWGG 2 cut(s) 216, 380
AluBI AGCT 2 cut(s) 7, 46
AluI AGCT 2 cut(s) 7, 46
Alw21I GWGCWC 1 cut(s) 9
Alw26I GTCTC 1 cut(s) 186
AlwI GGATC 1 cut(s) 85
Ama87I CYCGRG 1 cut(s) 8
AoxI GGCC 4 cut(s) 193, 219, 284, 383
ApaI GGGCCC 1 cut(s) 197
Asp700I GAANNNNTTC 1 cut(s) 236
Asp718I GGTACC 1 cut(s) 103
AspS9I GGNCC 3 cut(s) 116, 193, 194
AsuHPI GGTGA 3 cut(s) 170, 304, 333
AsuNHI GCTAGC 1 cut(s) 431
AvaI CYCGRG 1 cut(s) 8
AvaII GGWCC 1 cut(s) 116
BaeGI GKGCMC 1 cut(s) 197
BanI GGYRCC 1 cut(s) 103
BanII GRGCYC 2 cut(s) 9, 197
Bbv12I GWGCWC 1 cut(s) 9
BccI CCATC 2 cut(s) 222, 394
BciT130I CCWGG 2 cut(s) 218, 382
BcoDI GTCTC 1 cut(s) 186
BfaI CTAG 4 cut(s) 416, 432, 440, 448
BfmI CTRYAG 1 cut(s) 396
BfrI CTTAAG 1 cut(s) 47
BfuAI ACCTGC 2 cut(s) 104, 403
BglII AGATCT 1 cut(s) 58
BisI GCNGC 1 cut(s) 429
BlsI GCNGC 1 cut(s) 430
Bme1390I CCNGG 2 cut(s) 218, 382
Bme18I GGWCC 1 cut(s) 116
BmeT110I CYCGRG 1 cut(s) 8
BmgT120I GGNCC 3 cut(s) 116, 193, 194
BmiI GGNNCC 3 cut(s) 105, 195, 256
BmrFI CCNGG 2 cut(s) 218, 382
BmsI GCATC 1 cut(s) 330
BmtI GCTAGC 1 cut(s) 435
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 3 cut(s) 100, 113, 228
Bse1I ACTGG 2 cut(s) 209, 325
BseBI CCWGG 2 cut(s) 218, 382
BseGI GGATG 2 cut(s) 233, 321
BseLI CCNNNNNNNGG 3 cut(s) 100, 113, 228
BseMII CTCAG 1 cut(s) 71
BseNI ACTGG 2 cut(s) 209, 325
BseRI GAGGAG 1 cut(s) 267
BseSI GKGCMC 1 cut(s) 197
BsgI GTGCAG 1 cut(s) 375
BshFI GGCC 4 cut(s) 195, 221, 286, 385
BshNI GGYRCC 1 cut(s) 103
BsiHKAI GWGCWC 1 cut(s) 9
BsiHKCI CYCGRG 1 cut(s) 8
BsiSI CCGG 1 cut(s) 287
BslI CCNNNNNNNGG 3 cut(s) 100, 113, 228
BsmAI GTCTC 1 cut(s) 186
BsmI GAATGC 1 cut(s) 301
BsnI GGCC 4 cut(s) 195, 221, 286, 385
BsoBI CYCGRG 1 cut(s) 8
Bsp120I GGGCCC 1 cut(s) 193
Bsp1286I GDGCHC 2 cut(s) 9, 197
Bsp143I GATC 2 cut(s) 58, 90
BspACI CCGC 3 cut(s) 319, 428, 435
BspANI GGCC 4 cut(s) 195, 221, 286, 385
BspCNI CTCAG 1 cut(s) 72
BspLI GGNNCC 3 cut(s) 105, 195, 256
BspMAI CTGCAG 1 cut(s) 400
BspMI ACCTGC 2 cut(s) 104, 403
BspOI GCTAGC 1 cut(s) 435
BspPI GGATC 1 cut(s) 85
BspT107I GGYRCC 1 cut(s) 103
BspTI CTTAAG 1 cut(s) 47
BsrI ACTGG 2 cut(s) 209, 325
BssMI GATC 2 cut(s) 58, 90
Bst2UI CCWGG 2 cut(s) 218, 382
BstAFI CTTAAG 1 cut(s) 47
BstC8I GCNNGC 1 cut(s) 433
BstDEI CTNAG 2 cut(s) 80, 185
BstENI CCTNNNNNAGG 1 cut(s) 111
BstF5I GGATG 2 cut(s) 233, 321
BstKTI GATC 2 cut(s) 61, 93
BstMAI GTCTC 1 cut(s) 186
BstMBI GATC 2 cut(s) 58, 90
BstMWI GCNNNNNNNGC 1 cut(s) 425
BstNI CCWGG 2 cut(s) 218, 382
BstSCI CCNGG 2 cut(s) 216, 380
BstSFI CTRYAG 1 cut(s) 396
BstSLI GKGCMC 1 cut(s) 197
BstX2I RGATCY 2 cut(s) 58, 90
BstYI RGATCY 2 cut(s) 58, 90
BsuRI GGCC 4 cut(s) 195, 221, 286, 385
BtsCI GGATG 2 cut(s) 233, 321
BveI ACCTGC 2 cut(s) 104, 403
Cac8I GCNNGC 1 cut(s) 433
Cfr13I GGNCC 3 cut(s) 116, 193, 194
Csp6I GTAC 4 cut(s) 13, 77, 104, 309
CviAII CATG 3 cut(s) 100, 110, 407
CviJI RGCY 9 cut(s) 7, 46, 52, 195, 221, 257, 286, 385, 431
CviKI_1 RGCY 9 cut(s) 7, 46, 52, 195, 221, 257, 286, 385, 431
CviQI GTAC 4 cut(s) 13, 77, 104, 309
DdeI CTNAG 2 cut(s) 80, 185
DpnI GATC 2 cut(s) 60, 92
DpnII GATC 2 cut(s) 58, 90
EaeI YGGCCR 1 cut(s) 284
Ecl136II GAGCTC 1 cut(s) 7
Eco24I GRGCYC 2 cut(s) 9, 197
Eco47I GGWCC 1 cut(s) 116
Eco53kI GAGCTC 1 cut(s) 7
Eco88I CYCGRG 1 cut(s) 8
EcoICRI GAGCTC 1 cut(s) 7
EcoNI CCTNNNNNAGG 1 cut(s) 111
EcoO109I RGGNCCY 1 cut(s) 193
EcoRII CCWGG 2 cut(s) 216, 380
EcoT38I GRGCYC 2 cut(s) 9, 197
FaeI CATG 3 cut(s) 103, 113, 410
FaiI YATR 3 cut(s) 101, 111, 408
FatI CATG 3 cut(s) 99, 109, 406
Fnu4HI GCNGC 1 cut(s) 429
FokI GGATG 2 cut(s) 240, 308
FriOI GRGCYC 2 cut(s) 9, 197
Fsp4HI GCNGC 1 cut(s) 429
FspBI CTAG 4 cut(s) 416, 432, 440, 448
GluI GCNGC 1 cut(s) 429
HaeIII GGCC 4 cut(s) 195, 221, 286, 385
HapII CCGG 1 cut(s) 287
Hin1II CATG 3 cut(s) 103, 113, 410
HpaII CCGG 1 cut(s) 287
HphI GGTGA 3 cut(s) 170, 304, 333
Hpy188I TCNGA 1 cut(s) 140
Hpy188III TCNNGA 2 cut(s) 62, 119
HpyAV CCTTC 3 cut(s) 35, 104, 359
HpyCH4IV ACGT 1 cut(s) 124
HpyCH4V TGCA 4 cut(s) 113, 299, 392, 398
HpyF10VI GCNNNNNNNGC 1 cut(s) 425
HpyF3I CTNAG 2 cut(s) 80, 185
HpySE526I ACGT 1 cut(s) 124
Hsp92II CATG 3 cut(s) 103, 113, 410
KpnI GGTACC 1 cut(s) 107
Kzo9I GATC 2 cut(s) 58, 90
LmnI GCTCC 2 cut(s) 4, 254
LweI GCATC 1 cut(s) 330
MaeI CTAG 4 cut(s) 416, 432, 440, 448
MaeII ACGT 1 cut(s) 124
MaeIII GTNAC 2 cut(s) 125, 158
MalI GATC 2 cut(s) 60, 92
MboI GATC 2 cut(s) 58, 90
MboII GAAGA 1 cut(s) 369
MflI RGATCY 2 cut(s) 58, 90
MhlI GDGCHC 2 cut(s) 9, 197
MluCI AATT 3 cut(s) 261, 300, 330
MmeI TCCRAC 1 cut(s) 126
MnlI CCTC 5 cut(s) 117, 148, 245, 283, 394
MroXI GAANNNNTTC 1 cut(s) 236
MseI TTAA 3 cut(s) 48, 303, 329
MspCI CTTAAG 1 cut(s) 47
MspI CCGG 1 cut(s) 287
MspR9I CCNGG 2 cut(s) 218, 382
Mva1269I GAATGC 1 cut(s) 301
MvaI CCWGG 2 cut(s) 218, 382
MwoI GCNNNNNNNGC 1 cut(s) 425
NdeII GATC 2 cut(s) 58, 90
NheI GCTAGC 1 cut(s) 431
NlaIII CATG 3 cut(s) 103, 113, 410
NlaIV GGNNCC 3 cut(s) 105, 195, 256
NmuCI GTSAC 2 cut(s) 125, 158
PaeR7I CTCGAG 1 cut(s) 8
PaqCI CACCTGC 1 cut(s) 403
PctI GAATGC 1 cut(s) 301
PdmI GAANNNNTTC 1 cut(s) 236
PflMI CCANNNNNTGG 1 cut(s) 228
PkrI GCNGC 1 cut(s) 430
Psp124BI GAGCTC 1 cut(s) 9
Psp6I CCWGG 2 cut(s) 216, 380
PspGI CCWGG 2 cut(s) 216, 380
PspN4I GGNNCC 3 cut(s) 105, 195, 256
PspOMI GGGCCC 1 cut(s) 193
PspPI GGNCC 3 cut(s) 116, 193, 194
PspXI VCTCGAGB 1 cut(s) 8
PstI CTGCAG 1 cut(s) 400
PsuI RGATCY 2 cut(s) 58, 90
RsaI GTAC 4 cut(s) 14, 78, 105, 310
RsaNI GTAC 4 cut(s) 13, 77, 104, 309
SacI GAGCTC 1 cut(s) 9
SaqAI TTAA 3 cut(s) 48, 303, 329
SatI GCNGC 1 cut(s) 429
Sau3AI GATC 2 cut(s) 58, 90
Sau96I GGNCC 3 cut(s) 116, 193, 194
ScrFI CCNGG 2 cut(s) 218, 382
SduI GDGCHC 2 cut(s) 9, 197
SfaNI GCATC 1 cut(s) 330
SfcI CTRYAG 1 cut(s) 396
Sfr274I CTCGAG 1 cut(s) 8
SinI GGWCC 1 cut(s) 116
SlaI CTCGAG 1 cut(s) 8
SmlI CTYRAG 2 cut(s) 8, 47
SmoI CTYRAG 2 cut(s) 8, 47
Sse9I AATT 3 cut(s) 261, 300, 330
SsiI CCGC 3 cut(s) 319, 428, 435
SspMI CTAG 4 cut(s) 416, 432, 440, 448
SstI GAGCTC 1 cut(s) 9
StyD4I CCNGG 2 cut(s) 216, 380
TaiI ACGT 1 cut(s) 127
TaqI TCGA 1 cut(s) 9
TasI AATT 3 cut(s) 261, 300, 330
TatI WGTACW 1 cut(s) 76
TauI GCSGC 1 cut(s) 431
Tru1I TTAA 3 cut(s) 48, 303, 329
Tru9I TTAA 3 cut(s) 48, 303, 329
TseFI GTSAC 2 cut(s) 125, 158
Tsp45I GTSAC 2 cut(s) 125, 158
TspDTI ATGAA 3 cut(s) 229, 363, 423
Van91I CCANNNNNTGG 1 cut(s) 228
Vha464I CTTAAG 1 cut(s) 47
VpaK11BI GGWCC 1 cut(s) 116
XagI CCTNNNNNAGG 1 cut(s) 111
XhoI CTCGAG 1 cut(s) 8
XmnI GAANNNNTTC 1 cut(s) 236
XspI CTAG 4 cut(s) 416, 432, 440, 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.