RLG00000026144

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000707
Physical Location & Seq
Forward (+)
791060 .. 791401
342 bp
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UTR
Exon/CDS
Intron
RLM00000026144

Sequence Viewer

Length: 342 bp
ATGGAGCTTGAGTACCAGAGAGGCCGTGCTGTGAAAAAGCTGACCTCATCAGATGTGGGTAGCAGACTCGTAGTGACTAATGAGATGCTTCACATGCTGCCTCGAGCAGATCCAGAACACGACACTCCAATCAGAGTGTTGGACGATAAGGGTGACGAGTATCAGTTCTACTTGTCTATAAGAAAGGGCCCAAATCCCAAACCAGTTTTCCAGGCCAAAGGATGGAAAGCATTCATCAAAAAACGAGGAGCCAAACTTGACGACTACATCTGTTTCTGGCTAGAGGTGAATGCAATTAAGCGTACAGATTACCGCATCAAGTTGGTTCCATTCACACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

114

Amino Acids

13.21

Weight (kDa)

9.52

Isoelectric Point (pI)

13.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 11 - 95 1.8e-06 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 222
AciI CCGC 1 cut(s) 313
AclWI GGATC 1 cut(s) 104
AfaI GTAC 2 cut(s) 14, 304
AfiI CCNNNNNNNGG 1 cut(s) 222
AjnI CCWGG 1 cut(s) 210
AjuI GAANNNNNNNTTGG 2 cut(s) 191, 223
AluBI AGCT 2 cut(s) 7, 40
AluI AGCT 2 cut(s) 7, 40
AlwI GGATC 1 cut(s) 104
Ama87I CYCGRG 1 cut(s) 102
AoxI GGCC 3 cut(s) 22, 187, 213
ApaI GGGCCC 1 cut(s) 191
ApeKI GCWGC 1 cut(s) 97
Asp700I GAANNNNTTC 1 cut(s) 230
AspS9I GGNCC 2 cut(s) 187, 188
AsuHPI GGTGA 2 cut(s) 164, 298
AvaI CYCGRG 1 cut(s) 102
BaeGI GKGCMC 1 cut(s) 191
BanII GRGCYC 1 cut(s) 191
BbvI GCAGC 1 cut(s) 84
BccI CCATC 1 cut(s) 216
BceAI ACGGC 1 cut(s) 9
BciT130I CCWGG 1 cut(s) 212
BfaI CTAG 1 cut(s) 281
BisI GCNGC 1 cut(s) 98
BlsI GCNGC 1 cut(s) 99
Bme1390I CCNGG 1 cut(s) 212
BmeT110I CYCGRG 1 cut(s) 102
BmgT120I GGNCC 2 cut(s) 187, 188
BmiI GGNNCC 3 cut(s) 189, 250, 327
BmrFI CCNGG 1 cut(s) 212
BmsI GCATC 2 cut(s) 75, 324
BpuEI CTTGAG 1 cut(s) 29
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 1 cut(s) 222
Bse1I ACTGG 1 cut(s) 203
BseBI CCWGG 1 cut(s) 212
BseGI GGATG 1 cut(s) 227
BseLI CCNNNNNNNGG 1 cut(s) 222
BseNI ACTGG 1 cut(s) 203
BseRI GAGGAG 1 cut(s) 261
BseSI GKGCMC 1 cut(s) 191
BseXI GCAGC 1 cut(s) 84
BshFI GGCC 3 cut(s) 24, 189, 215
BsiHKCI CYCGRG 1 cut(s) 102
BslI CCNNNNNNNGG 1 cut(s) 222
BsmI GAATGC 2 cut(s) 230, 295
BsnI GGCC 3 cut(s) 24, 189, 215
BsoBI CYCGRG 1 cut(s) 102
Bsp120I GGGCCC 1 cut(s) 187
Bsp1286I GDGCHC 1 cut(s) 191
Bsp143I GATC 1 cut(s) 109
BspACI CCGC 1 cut(s) 313
BspANI GGCC 3 cut(s) 24, 189, 215
BspLI GGNNCC 3 cut(s) 189, 250, 327
BspPI GGATC 1 cut(s) 104
BsrI ACTGG 1 cut(s) 203
BssMI GATC 1 cut(s) 109
Bst2UI CCWGG 1 cut(s) 212
BstF5I GGATG 1 cut(s) 227
BstKTI GATC 1 cut(s) 112
BstMBI GATC 1 cut(s) 109
BstMWI GCNNNNNNNGC 1 cut(s) 94
BstNI CCWGG 1 cut(s) 212
BstNSI RCATGY 1 cut(s) 97
BstSCI CCNGG 1 cut(s) 210
BstSLI GKGCMC 1 cut(s) 191
BstV1I GCAGC 1 cut(s) 84
BstX2I RGATCY 1 cut(s) 109
BstYI RGATCY 1 cut(s) 109
BsuRI GGCC 3 cut(s) 24, 189, 215
BtsCI GGATG 1 cut(s) 227
Cfr13I GGNCC 2 cut(s) 187, 188
Csp6I GTAC 2 cut(s) 13, 303
CviAII CATG 1 cut(s) 94
CviJI RGCY 7 cut(s) 7, 24, 40, 189, 215, 251, 280
CviKI_1 RGCY 7 cut(s) 7, 24, 40, 189, 215, 251, 280
CviQI GTAC 2 cut(s) 13, 303
DpnI GATC 1 cut(s) 111
DpnII GATC 1 cut(s) 109
Eco24I GRGCYC 1 cut(s) 191
Eco88I CYCGRG 1 cut(s) 102
EcoO109I RGGNCCY 1 cut(s) 187
EcoRII CCWGG 1 cut(s) 210
EcoT38I GRGCYC 1 cut(s) 191
FaeI CATG 1 cut(s) 97
FaiI YATR 2 cut(s) 95, 179
FatI CATG 1 cut(s) 93
Fnu4HI GCNGC 1 cut(s) 98
FokI GGATG 1 cut(s) 234
FriOI GRGCYC 1 cut(s) 191
Fsp4HI GCNGC 1 cut(s) 98
FspBI CTAG 1 cut(s) 281
GluI GCNGC 1 cut(s) 98
HaeIII GGCC 3 cut(s) 24, 189, 215
Hin1II CATG 1 cut(s) 97
HinfI GANTC 1 cut(s) 66
HphI GGTGA 2 cut(s) 164, 298
Hpy188I TCNGA 2 cut(s) 52, 134
Hpy188III TCNNGA 1 cut(s) 113
HpyCH4V TGCA 1 cut(s) 293
HpyF10VI GCNNNNNNNGC 1 cut(s) 94
Hsp92II CATG 1 cut(s) 97
Kzo9I GATC 1 cut(s) 109
LmnI GCTCC 2 cut(s) 4, 248
LpnPI CCDG 6 cut(s) 29, 126, 197, 216, 224, 262
Lsp1109I GCAGC 1 cut(s) 84
LweI GCATC 2 cut(s) 75, 324
MaeI CTAG 1 cut(s) 281
MaeIII GTNAC 2 cut(s) 73, 152
MalI GATC 1 cut(s) 111
MboI GATC 1 cut(s) 109
MflI RGATCY 1 cut(s) 109
MhlI GDGCHC 1 cut(s) 191
MluCI AATT 1 cut(s) 294
MlyI GAGTC 1 cut(s) 60
MmeI TCCRAC 1 cut(s) 120
MnlI CCTC 5 cut(s) 14, 55, 111, 239, 277
MroXI GAANNNNTTC 1 cut(s) 230
MseI TTAA 2 cut(s) 297, 340
MspR9I CCNGG 1 cut(s) 212
Mva1269I GAATGC 2 cut(s) 230, 295
MvaI CCWGG 1 cut(s) 212
MwoI GCNNNNNNNGC 1 cut(s) 94
NdeII GATC 1 cut(s) 109
NlaIII CATG 1 cut(s) 97
NlaIV GGNNCC 3 cut(s) 189, 250, 327
NmuCI GTSAC 2 cut(s) 73, 152
NspI RCATGY 1 cut(s) 97
PaeR7I CTCGAG 1 cut(s) 102
PctI GAATGC 2 cut(s) 230, 295
PdmI GAANNNNTTC 1 cut(s) 230
PflMI CCANNNNNTGG 1 cut(s) 222
PkrI GCNGC 1 cut(s) 99
PleI GAGTC 1 cut(s) 60
PpsI GAGTC 1 cut(s) 60
Psp6I CCWGG 1 cut(s) 210
PspGI CCWGG 1 cut(s) 210
PspN4I GGNNCC 3 cut(s) 189, 250, 327
PspOMI GGGCCC 1 cut(s) 187
PspPI GGNCC 2 cut(s) 187, 188
PspXI VCTCGAGB 1 cut(s) 102
PsuI RGATCY 1 cut(s) 109
RsaI GTAC 2 cut(s) 14, 304
RsaNI GTAC 2 cut(s) 13, 303
SaqAI TTAA 2 cut(s) 297, 340
SatI GCNGC 1 cut(s) 98
Sau3AI GATC 1 cut(s) 109
Sau96I GGNCC 2 cut(s) 187, 188
SchI GAGTC 1 cut(s) 60
ScrFI CCNGG 1 cut(s) 212
SduI GDGCHC 1 cut(s) 191
SetI ASST 4 cut(s) 9, 42, 47, 288
SfaNI GCATC 2 cut(s) 75, 324
Sfr274I CTCGAG 1 cut(s) 102
SlaI CTCGAG 1 cut(s) 102
SmlI CTYRAG 2 cut(s) 8, 102
SmoI CTYRAG 2 cut(s) 8, 102
Sse9I AATT 1 cut(s) 294
SsiI CCGC 1 cut(s) 313
SspMI CTAG 1 cut(s) 281
StyD4I CCNGG 1 cut(s) 210
TaqI TCGA 1 cut(s) 103
TasI AATT 1 cut(s) 294
Tru1I TTAA 2 cut(s) 297, 340
Tru9I TTAA 2 cut(s) 297, 340
TseFI GTSAC 2 cut(s) 73, 152
TseI GCWGC 1 cut(s) 97
Tsp45I GTSAC 2 cut(s) 73, 152
TspDTI ATGAA 1 cut(s) 223
Van91I CCANNNNNTGG 1 cut(s) 222
XceI RCATGY 1 cut(s) 97
XhoI CTCGAG 1 cut(s) 102
XmnI GAANNNNTTC 1 cut(s) 230
XspI CTAG 1 cut(s) 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.