Reverse transcriptase (RNA-dependent DNA polymerase)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 288 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 803 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 1548, 2265 |
| AccI | GTMKAC | 2 cut(s) | 1617, 2222 |
| AclI | AACGTT | 1 cut(s) | 1328 |
| AclWI | GGATC | 3 cut(s) | 1070, 1098, 2564 |
| AcoI | YGGCCR | 1 cut(s) | 559 |
| AcsI | RAATTY | 5 cut(s) | 445, 861, 1052, 2362, 2628 |
| AcuI | CTGAAG | 2 cut(s) | 224, 1269 |
| AdeI | CACNNNGTG | 1 cut(s) | 1768 |
| AfaI | GTAC | 8 cut(s) | 77, 161, 829, 1229, 2011, 2119, 2495, 2551 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 1548, 1973, 2265 |
| AhlI | ACTAGT | 1 cut(s) | 1577 |
| AjnI | CCWGG | 2 cut(s) | 1541, 2048 |
| AleI | CACNNNNGTG | 1 cut(s) | 2005 |
| Alw21I | GWGCWC | 1 cut(s) | 476 |
| Alw26I | GTCTC | 2 cut(s) | 1164, 1785 |
| AlwI | GGATC | 3 cut(s) | 1070, 1098, 2564 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 35 |
| AoxI | GGCC | 3 cut(s) | 559, 2015, 2402 |
| ApeKI | GCWGC | 4 cut(s) | 818, 1462, 1465, 2597 |
| ApoI | RAATTY | 5 cut(s) | 445, 861, 1052, 2362, 2628 |
| AseI | ATTAAT | 1 cut(s) | 1836 |
| AspS9I | GGNCC | 1 cut(s) | 527 |
| AsuHPI | GGTGA | 2 cut(s) | 1913, 2422 |
| AsuII | TTCGAA | 1 cut(s) | 2331 |
| AvaII | GGWCC | 1 cut(s) | 527 |
| BalI | TGGCCA | 1 cut(s) | 561 |
| BanII | GRGCYC | 1 cut(s) | 476 |
| BbsI | GAAGAC | 1 cut(s) | 1455 |
| Bbv12I | GWGCWC | 1 cut(s) | 476 |
| BbvI | GCAGC | 4 cut(s) | 830, 1449, 1477, 2609 |
| BccI | CCATC | 8 cut(s) | 10, 279, 338, 407, 575, 715, 1363, 2616 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 2321, 2355 |
| BciT130I | CCWGG | 2 cut(s) | 1543, 2050 |
| BciVI | GTATCC | 2 cut(s) | 1286, 2225 |
| BcoDI | GTCTC | 2 cut(s) | 1164, 1785 |
| BcuI | ACTAGT | 1 cut(s) | 1577 |
| BfaI | CTAG | 6 cut(s) | 726, 1020, 1578, 1772, 1929, 2274 |
| BfmI | CTRYAG | 1 cut(s) | 1463 |
| BfuI | GTATCC | 2 cut(s) | 1286, 2225 |
| BglII | AGATCT | 1 cut(s) | 1874 |
| BisI | GCNGC | 4 cut(s) | 819, 1463, 1466, 2598 |
| BlsI | GCNGC | 4 cut(s) | 820, 1464, 1467, 2599 |
| BmcAI | AGTACT | 1 cut(s) | 2495 |
| Bme1390I | CCNGG | 2 cut(s) | 1543, 2050 |
| Bme18I | GGWCC | 1 cut(s) | 527 |
| BmgT120I | GGNCC | 1 cut(s) | 527 |
| BmiI | GGNNCC | 2 cut(s) | 222, 2257 |
| BmrFI | CCNGG | 2 cut(s) | 1543, 2050 |
| BmrI | ACTGGG | 1 cut(s) | 1331 |
| BmsI | GCATC | 5 cut(s) | 893, 1234, 1342, 1555, 2176 |
| BmuI | ACTGGG | 1 cut(s) | 1331 |
| BpiI | GAAGAC | 1 cut(s) | 1455 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 311, 343 |
| BpmI | CTGGAG | 4 cut(s) | 498, 915, 1961, 2032 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1287 |
| Bpu14I | TTCGAA | 1 cut(s) | 2331 |
| BsaJI | CCNNGG | 1 cut(s) | 618 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 749, 779 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 1548, 1973, 2265 |
| Bse1I | ACTGG | 8 cut(s) | 130, 562, 599, 898, 977, 1337, 1683, 2591 |
| Bse3DI | GCAATG | 3 cut(s) | 1140, 1553, 2319 |
| BseBI | CCWGG | 2 cut(s) | 1543, 2050 |
| BseDI | CCNNGG | 1 cut(s) | 618 |
| BseGI | GGATG | 8 cut(s) | 418, 726, 1147, 1220, 1417, 1612, 1672, 2215 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 1548, 1973, 2265 |
| BseMI | GCAATG | 3 cut(s) | 1140, 1553, 2319 |
| BseMII | CTCAG | 4 cut(s) | 87, 177, 185, 308 |
| BseNI | ACTGG | 8 cut(s) | 130, 562, 599, 898, 977, 1337, 1683, 2591 |
| BseRI | GAGGAG | 2 cut(s) | 233, 2268 |
| BseXI | GCAGC | 4 cut(s) | 830, 1449, 1477, 2609 |
| BseYI | CCCAGC | 2 cut(s) | 1810, 2066 |
| BsgI | GTGCAG | 2 cut(s) | 359, 2325 |
| BshFI | GGCC | 3 cut(s) | 561, 2017, 2404 |
| BsiHKAI | GWGCWC | 1 cut(s) | 476 |
| BsiSI | CCGG | 1 cut(s) | 1469 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 1548, 1973, 2265 |
| BsmAI | GTCTC | 2 cut(s) | 1164, 1785 |
| BsmI | GAATGC | 2 cut(s) | 64, 1888 |
| BsnI | GGCC | 3 cut(s) | 561, 2017, 2404 |
| Bsp119I | TTCGAA | 1 cut(s) | 2331 |
| Bsp1286I | GDGCHC | 1 cut(s) | 476 |
| Bsp1407I | TGTACA | 2 cut(s) | 1227, 2009 |
| Bsp143I | GATC | 9 cut(s) | 490, 637, 1075, 1103, 1518, 1626, 1874, 2155, 2569 |
| BspANI | GGCC | 3 cut(s) | 561, 2017, 2404 |
| BspCNI | CTCAG | 4 cut(s) | 88, 178, 186, 307 |
| BspHI | TCATGA | 2 cut(s) | 165, 2158 |
| BspLI | GGNNCC | 2 cut(s) | 222, 2257 |
| BspMAI | CTGCAG | 1 cut(s) | 1467 |
| BspPI | GGATC | 3 cut(s) | 1070, 1098, 2564 |
| BspT104I | TTCGAA | 1 cut(s) | 2331 |
| BsrDI | GCAATG | 3 cut(s) | 1140, 1553, 2319 |
| BsrGI | TGTACA | 2 cut(s) | 1227, 2009 |
| BsrI | ACTGG | 8 cut(s) | 130, 562, 599, 898, 977, 1337, 1683, 2591 |
| BssECI | CCNNGG | 1 cut(s) | 618 |
| BssMI | GATC | 9 cut(s) | 490, 637, 1075, 1103, 1518, 1626, 1874, 2155, 2569 |
| BssNAI | GTATAC | 1 cut(s) | 1618 |
| BssT1I | CCWWGG | 1 cut(s) | 618 |
| Bst1107I | GTATAC | 1 cut(s) | 1618 |
| Bst2UI | CCWGG | 2 cut(s) | 1543, 2050 |
| Bst4CI | ACNGT | 4 cut(s) | 800, 832, 1045, 1115 |
| Bst6I | CTCTTC | 2 cut(s) | 342, 1587 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 182, 2603 |
| BstAUI | TGTACA | 2 cut(s) | 1227, 2009 |
| BstBI | TTCGAA | 1 cut(s) | 2331 |
| BstC8I | GCNNGC | 6 cut(s) | 506, 1131, 1975, 2247, 2279, 2565 |
| BstDEI | CTNAG | 9 cut(s) | 96, 186, 194, 294, 360, 396, 1236, 1287, 1713 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1971 |
| BstF5I | GGATG | 8 cut(s) | 418, 726, 1147, 1220, 1417, 1612, 1672, 2215 |
| BstKTI | GATC | 9 cut(s) | 493, 640, 1078, 1106, 1521, 1629, 1877, 2158, 2572 |
| BstMAI | GTCTC | 2 cut(s) | 1164, 1785 |
| BstMBI | GATC | 9 cut(s) | 490, 637, 1075, 1103, 1518, 1626, 1874, 2155, 2569 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 182, 199, 414, 1379, 1918, 2255, 2603 |
| BstNI | CCWGG | 2 cut(s) | 1543, 2050 |
| BstNSI | RCATGY | 2 cut(s) | 1159, 2348 |
| BstSCI | CCNGG | 2 cut(s) | 1541, 2048 |
| BstSFI | CTRYAG | 1 cut(s) | 1463 |
| BstV1I | GCAGC | 4 cut(s) | 830, 1449, 1477, 2609 |
| BstV2I | GAAGAC | 1 cut(s) | 1455 |
| BstX2I | RGATCY | 2 cut(s) | 1874, 2569 |
| BstYI | RGATCY | 2 cut(s) | 1874, 2569 |
| BstZ17I | GTATAC | 1 cut(s) | 1618 |
| BsuI | GTATCC | 2 cut(s) | 1286, 2225 |
| BsuRI | GGCC | 3 cut(s) | 561, 2017, 2404 |
| BtsCI | GGATG | 8 cut(s) | 418, 726, 1147, 1220, 1417, 1612, 1672, 2215 |
| BtsI | GCAGTG | 1 cut(s) | 1928 |
| BtsIMutI | CAGTG | 4 cut(s) | 530, 805, 1636, 1928 |
| Cac8I | GCNNGC | 6 cut(s) | 506, 1131, 1975, 2247, 2279, 2565 |
| CaiI | CAGNNNCTG | 1 cut(s) | 35 |
| CciI | TCATGA | 2 cut(s) | 165, 2158 |
| Cfr13I | GGNCC | 1 cut(s) | 527 |
| Csp6I | GTAC | 8 cut(s) | 76, 160, 828, 1228, 2010, 2118, 2494, 2550 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 1263, 1298, 1612, 1647 |
| CviQI | GTAC | 8 cut(s) | 76, 160, 828, 1228, 2010, 2118, 2494, 2550 |
| DdeI | CTNAG | 9 cut(s) | 96, 186, 194, 294, 360, 396, 1236, 1287, 1713 |
| DpnI | GATC | 9 cut(s) | 492, 639, 1077, 1105, 1520, 1628, 1876, 2157, 2571 |
| DpnII | GATC | 9 cut(s) | 490, 637, 1075, 1103, 1518, 1626, 1874, 2155, 2569 |
| DraI | TTTAAA | 4 cut(s) | 1203, 1431, 1557, 2217 |
| DraIII | CACNNNGTG | 1 cut(s) | 1768 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 803 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 803 |
| EaeI | YGGCCR | 1 cut(s) | 559 |
| Eam1104I | CTCTTC | 2 cut(s) | 342, 1587 |
| EarI | CTCTTC | 2 cut(s) | 342, 1587 |
| Ecl136II | GAGCTC | 1 cut(s) | 474 |
| Eco130I | CCWWGG | 1 cut(s) | 618 |
| Eco147I | AGGCCT | 1 cut(s) | 2017 |
| Eco24I | GRGCYC | 1 cut(s) | 476 |
| Eco32I | GATATC | 1 cut(s) | 1672 |
| Eco47I | GGWCC | 1 cut(s) | 527 |
| Eco53kI | GAGCTC | 1 cut(s) | 474 |
| Eco57I | CTGAAG | 2 cut(s) | 224, 1269 |
| EcoICRI | GAGCTC | 1 cut(s) | 474 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1971 |
| EcoRII | CCWGG | 2 cut(s) | 1541, 2048 |
| EcoRV | GATATC | 1 cut(s) | 1672 |
| EcoT14I | CCWWGG | 1 cut(s) | 618 |
| EcoT22I | ATGCAT | 1 cut(s) | 1914 |
| EcoT38I | GRGCYC | 1 cut(s) | 476 |
| ErhI | CCWWGG | 1 cut(s) | 618 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 1263, 1295, 1409, 1441, 1706, 1738 |
| FauNDI | CATATG | 2 cut(s) | 939, 2622 |
| FblI | GTMKAC | 2 cut(s) | 1617, 2222 |
| Fnu4HI | GCNGC | 4 cut(s) | 819, 1463, 1466, 2598 |
| FokI | GGATG | 8 cut(s) | 425, 733, 1154, 1227, 1424, 1619, 1679, 2222 |
| FriOI | GRGCYC | 1 cut(s) | 476 |
| Fsp4HI | GCNGC | 4 cut(s) | 819, 1463, 1466, 2598 |
| FspBI | CTAG | 6 cut(s) | 726, 1020, 1578, 1772, 1929, 2274 |
| GluI | GCNGC | 4 cut(s) | 819, 1463, 1466, 2598 |
| GsaI | CCCAGC | 2 cut(s) | 1814, 2070 |
| GsuI | CTGGAG | 4 cut(s) | 498, 915, 1961, 2032 |
| HaeIII | GGCC | 3 cut(s) | 561, 2017, 2404 |
| HapII | CCGG | 1 cut(s) | 1469 |
| HincII | GTYRAC | 1 cut(s) | 2577 |
| HindII | GTYRAC | 1 cut(s) | 2577 |
| HindIII | AAGCTT | 3 cut(s) | 119, 1127, 1536 |
| HinfI | GANTC | 5 cut(s) | 32, 648, 713, 1636, 2390 |
| HpaII | CCGG | 1 cut(s) | 1469 |
| HphI | GGTGA | 2 cut(s) | 1913, 2422 |
| Hpy166II | GTNNAC | 6 cut(s) | 530, 1228, 1618, 2010, 2223, 2577 |
| Hpy188I | TCNGA | 8 cut(s) | 37, 276, 297, 495, 705, 778, 1108, 1214 |
| Hpy8I | GTNNAC | 6 cut(s) | 530, 1228, 1618, 2010, 2223, 2577 |
| HpyAV | CCTTC | 8 cut(s) | 292, 565, 981, 1244, 1301, 1466, 1738, 2374 |
| HpyCH4III | ACNGT | 4 cut(s) | 800, 832, 1045, 1115 |
| HpyCH4IV | ACGT | 1 cut(s) | 1328 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 182, 199, 414, 1379, 1918, 2255, 2603 |
| HpyF3I | CTNAG | 9 cut(s) | 96, 186, 194, 294, 360, 396, 1236, 1287, 1713 |
| HpySE526I | ACGT | 1 cut(s) | 1328 |
| Kzo9I | GATC | 9 cut(s) | 490, 637, 1075, 1103, 1518, 1626, 1874, 2155, 2569 |
| LmnI | GCTCC | 5 cut(s) | 173, 220, 845, 896, 2255 |
| Lsp1109I | GCAGC | 4 cut(s) | 830, 1449, 1477, 2609 |
| LweI | GCATC | 5 cut(s) | 893, 1234, 1342, 1555, 2176 |
| MaeI | CTAG | 6 cut(s) | 726, 1020, 1578, 1772, 1929, 2274 |
| MaeII | ACGT | 1 cut(s) | 1328 |
| MaeIII | GTNAC | 3 cut(s) | 805, 990, 1115 |
| MalI | GATC | 9 cut(s) | 492, 639, 1077, 1105, 1520, 1628, 1876, 2157, 2571 |
| MboI | GATC | 9 cut(s) | 490, 637, 1075, 1103, 1518, 1626, 1874, 2155, 2569 |
| MfeI | CAATTG | 1 cut(s) | 63 |
| MflI | RGATCY | 2 cut(s) | 1874, 2569 |
| MhlI | GDGCHC | 1 cut(s) | 476 |
| MlsI | TGGCCA | 1 cut(s) | 561 |
| MluNI | TGGCCA | 1 cut(s) | 561 |
| MlyI | GAGTC | 1 cut(s) | 1645 |
| MmeI | TCCRAC | 3 cut(s) | 1103, 1675, 1963 |
| Mox20I | TGGCCA | 1 cut(s) | 561 |
| Mph1103I | ATGCAT | 1 cut(s) | 1914 |
| MscI | TGGCCA | 1 cut(s) | 561 |
| MslI | CAYNNNNRTG | 5 cut(s) | 335, 535, 1457, 2005, 2240 |
| Msp20I | TGGCCA | 1 cut(s) | 561 |
| MspA1I | CMGCKG | 1 cut(s) | 2435 |
| MspI | CCGG | 1 cut(s) | 1469 |
| MspR9I | CCNGG | 2 cut(s) | 1543, 2050 |
| MunI | CAATTG | 1 cut(s) | 63 |
| Mva1269I | GAATGC | 2 cut(s) | 64, 1888 |
| MvaI | CCWGG | 2 cut(s) | 1543, 2050 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 182, 199, 414, 1379, 1918, 2255, 2603 |
| NdeI | CATATG | 2 cut(s) | 939, 2622 |
| NdeII | GATC | 9 cut(s) | 490, 637, 1075, 1103, 1518, 1626, 1874, 2155, 2569 |
| NlaIV | GGNNCC | 2 cut(s) | 222, 2257 |
| NmuCI | GTSAC | 3 cut(s) | 805, 990, 1115 |
| NsiI | ATGCAT | 1 cut(s) | 1914 |
| NspI | RCATGY | 2 cut(s) | 1159, 2348 |
| NspV | TTCGAA | 1 cut(s) | 2331 |
| OliI | CACNNNNGTG | 1 cut(s) | 2005 |
| PagI | TCATGA | 2 cut(s) | 165, 2158 |
| PceI | AGGCCT | 1 cut(s) | 2017 |
| PctI | GAATGC | 2 cut(s) | 64, 1888 |
| PfeI | GAWTC | 4 cut(s) | 32, 648, 713, 2390 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 1548, 2265 |
| PkrI | GCNGC | 4 cut(s) | 820, 1464, 1467, 2599 |
| PleI | GAGTC | 1 cut(s) | 1644 |
| PpsI | GAGTC | 1 cut(s) | 1644 |
| PshBI | ATTAAT | 1 cut(s) | 1836 |
| PsiI | TTATAA | 1 cut(s) | 288 |
| Psp124BI | GAGCTC | 1 cut(s) | 476 |
| Psp1406I | AACGTT | 1 cut(s) | 1328 |
| Psp6I | CCWGG | 2 cut(s) | 1541, 2048 |
| PspFI | CCCAGC | 2 cut(s) | 1810, 2066 |
| PspGI | CCWGG | 2 cut(s) | 1541, 2048 |
| PspN4I | GGNNCC | 2 cut(s) | 222, 2257 |
| PspPI | GGNCC | 1 cut(s) | 527 |
| PstI | CTGCAG | 1 cut(s) | 1467 |
| PstNI | CAGNNNCTG | 1 cut(s) | 35 |
| PsuI | RGATCY | 2 cut(s) | 1874, 2569 |
| PvuII | CAGCTG | 1 cut(s) | 2435 |
| RsaI | GTAC | 8 cut(s) | 77, 161, 829, 1229, 2011, 2119, 2495, 2551 |
| RsaNI | GTAC | 8 cut(s) | 76, 160, 828, 1228, 2010, 2118, 2494, 2550 |
| RseI | CAYNNNNRTG | 5 cut(s) | 335, 535, 1457, 2005, 2240 |
| SacI | GAGCTC | 1 cut(s) | 476 |
| SatI | GCNGC | 4 cut(s) | 819, 1463, 1466, 2598 |
| Sau3AI | GATC | 9 cut(s) | 490, 637, 1075, 1103, 1518, 1626, 1874, 2155, 2569 |
| Sau96I | GGNCC | 1 cut(s) | 527 |
| ScaI | AGTACT | 1 cut(s) | 2495 |
| SchI | GAGTC | 1 cut(s) | 1645 |
| ScrFI | CCNGG | 2 cut(s) | 1543, 2050 |
| SduI | GDGCHC | 1 cut(s) | 476 |
| SfaNI | GCATC | 5 cut(s) | 893, 1234, 1342, 1555, 2176 |
| SfcI | CTRYAG | 1 cut(s) | 1463 |
| SfuI | TTCGAA | 1 cut(s) | 2331 |
| SinI | GGWCC | 1 cut(s) | 527 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 335, 535, 1457, 2005, 2240 |
| SpeI | ACTAGT | 1 cut(s) | 1577 |
| SseBI | AGGCCT | 1 cut(s) | 2017 |
| SspI | AATATT | 1 cut(s) | 2482 |
| SspMI | CTAG | 6 cut(s) | 726, 1020, 1578, 1772, 1929, 2274 |
| SstI | GAGCTC | 1 cut(s) | 476 |
| StuI | AGGCCT | 1 cut(s) | 2017 |
| StyD4I | CCNGG | 2 cut(s) | 1541, 2048 |
| StyI | CCWWGG | 1 cut(s) | 618 |
| TaaI | ACNGT | 4 cut(s) | 800, 832, 1045, 1115 |
| TaiI | ACGT | 1 cut(s) | 1331 |
| TaqI | TCGA | 8 cut(s) | 470, 866, 1050, 1517, 1737, 1789, 2331, 2393 |
| TatI | WGTACW | 6 cut(s) | 75, 159, 827, 1227, 2009, 2493 |
| TfiI | GAWTC | 4 cut(s) | 32, 648, 713, 2390 |
| TscAI | CASTG | 4 cut(s) | 537, 805, 1636, 1928 |
| TseFI | GTSAC | 3 cut(s) | 805, 990, 1115 |
| TseI | GCWGC | 4 cut(s) | 818, 1462, 1465, 2597 |
| Tsp45I | GTSAC | 3 cut(s) | 805, 990, 1115 |
| TspGWI | ACGGA | 1 cut(s) | 2562 |
| TspRI | CASTG | 4 cut(s) | 537, 805, 1636, 1928 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 1548, 2265 |
| VpaK11BI | GGWCC | 1 cut(s) | 527 |
| VspI | ATTAAT | 1 cut(s) | 1836 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1971 |
| XapI | RAATTY | 5 cut(s) | 445, 861, 1052, 2362, 2628 |
| XceI | RCATGY | 2 cut(s) | 1159, 2348 |
| XmiI | GTMKAC | 2 cut(s) | 1617, 2222 |
| XspI | CTAG | 6 cut(s) | 726, 1020, 1578, 1772, 1929, 2274 |
| ZrmI | AGTACT | 1 cut(s) | 2495 |
| Zsp2I | ATGCAT | 1 cut(s) | 1914 |