pycom01g08240

Reverse transcriptase (RNA-dependent DNA polymerase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0001006
Physical Location & Seq
Reverse (-)
9189922 .. 9191028
1107 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g08240.2

Sequence Viewer

Length: 612 bp
ATGGTTTTATATGTGGATGATATACTGTTGGCAAGCTCTCATATGCAGTTAATGATTGAAACCAAGAAAATGCCATGTAACGGTTTTGATATGAAAGATTTGGGAGAGGCTCATTATGTTTTGGGTATTAAGATTGTGAGGAATAGGCAAAATGGAACCTTGGGGCTATCTCAGAAGAACTACATTGAGAGAGTGTTGAAGAGATTCAATATGGAAGCTTACAGTGCCGGTGATGTCCCCATTGGTAAAGGAGATAAGTTCTCTCTCTCACAGTGCCCTATAACTCAACATGACATTCGAGTTATGATGGATAAGCCGTATGAATCTCTAGTTGGCAGTGTCATGAATGCACAAGTATGCACAAGACCTGATTTAGCGTTTGCATTAAGTGTACTAGGAAGGTTTCAATCAAATCCTGGTATTGCACACTGGAATGCAGGCAAAAAGGTACTGAGGTATTTGAAAAAGACACAGGACTATGTATTAAGTTACCATCGAGTCCAAAACTTGGAAGTTGTCTATTATAGTGATGCTGATTTGAGAGGCTGCATTGATGATAAAATTTTTATACAAAATGATGTAGAGAACGGTTATGAGGAGTTGGAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2

Protein Analysis

204

Amino Acids

23.13

Weight (kDa)

6.51

Isoelectric Point (pI)

24.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 508
AcsI RAATTY 1 cut(s) 561
AfaI GTAC 2 cut(s) 393, 450
AfiI CCNNNNNNNGG 2 cut(s) 80, 508
AgsI TTSAA 5 cut(s) 59, 199, 208, 407, 463
AjnI CCWGG 1 cut(s) 415
AjuI GAANNNNNNNTTGG 2 cut(s) 315, 347
AluBI AGCT 2 cut(s) 36, 218
AluI AGCT 2 cut(s) 36, 218
ApeKI GCWGC 1 cut(s) 546
ApoI RAATTY 1 cut(s) 561
Asp700I GAANNNNTTC 1 cut(s) 203
AsuHPI GGTGA 1 cut(s) 242
BaeGI GKGCMC 1 cut(s) 278
BaeI ACNNNNGTAYC 2 cut(s) 440, 473
BbvI GCAGC 1 cut(s) 533
BccI CCATC 2 cut(s) 301, 501
BceAI ACGGC 1 cut(s) 301
BciT130I CCWGG 1 cut(s) 417
BfaI CTAG 2 cut(s) 329, 395
BisI GCNGC 1 cut(s) 547
BlsI GCNGC 1 cut(s) 548
Bme1390I CCNGG 1 cut(s) 417
BmiI GGNNCC 1 cut(s) 157
BmrFI CCNGG 1 cut(s) 417
BmsI GCATC 1 cut(s) 520
BsaJI CCNNGG 1 cut(s) 159
Bsc4I CCNNNNNNNGG 2 cut(s) 80, 508
Bse118I RCCGGY 1 cut(s) 227
Bse1I ACTGG 1 cut(s) 434
BseBI CCWGG 1 cut(s) 417
BseDI CCNNGG 1 cut(s) 159
BseGI GGATG 1 cut(s) 22
BseLI CCNNNNNNNGG 2 cut(s) 80, 508
BseMII CTCAG 2 cut(s) 185, 443
BseNI ACTGG 1 cut(s) 434
BseRI GAGGAG 1 cut(s) 611
BseSI GKGCMC 1 cut(s) 278
BseXI GCAGC 1 cut(s) 533
BsiSI CCGG 1 cut(s) 228
BslFI GGGAC 1 cut(s) 221
BslI CCNNNNNNNGG 2 cut(s) 80, 508
BsmFI GGGAC 1 cut(s) 221
BsmI GAATGC 2 cut(s) 352, 439
Bsp1286I GDGCHC 1 cut(s) 278
BspCNI CTCAG 2 cut(s) 184, 444
BspHI TCATGA 1 cut(s) 342
BspLI GGNNCC 1 cut(s) 157
BsrFI RCCGGY 1 cut(s) 227
BsrI ACTGG 1 cut(s) 434
BssAI RCCGGY 1 cut(s) 227
BssECI CCNNGG 1 cut(s) 159
BssT1I CCWWGG 1 cut(s) 159
Bst2UI CCWGG 1 cut(s) 417
Bst4CI ACNGT 5 cut(s) 27, 83, 224, 273, 590
Bst6I CTCTTC 1 cut(s) 194
BstC8I GCNNGC 2 cut(s) 34, 439
BstDEI CTNAG 2 cut(s) 171, 452
BstF5I GGATG 1 cut(s) 22
BstMWI GCNNNNNNNGC 1 cut(s) 224
BstNI CCWGG 1 cut(s) 417
BstSCI CCNGG 1 cut(s) 415
BstSLI GKGCMC 1 cut(s) 278
BstV1I GCAGC 1 cut(s) 533
BtsCI GGATG 1 cut(s) 22
BtsI GCAGTG 1 cut(s) 343
BtsIMutI CAGTG 4 cut(s) 229, 278, 343, 427
Cac8I GCNNGC 2 cut(s) 34, 439
CciI TCATGA 1 cut(s) 342
Cfr10I RCCGGY 1 cut(s) 227
Csp6I GTAC 2 cut(s) 392, 449
CviAII CATG 3 cut(s) 75, 290, 343
CviJI RGCY 6 cut(s) 36, 110, 166, 218, 316, 546
CviKI_1 RGCY 6 cut(s) 36, 110, 166, 218, 316, 546
CviQI GTAC 2 cut(s) 392, 449
DdeI CTNAG 2 cut(s) 171, 452
Eam1104I CTCTTC 1 cut(s) 194
EarI CTCTTC 1 cut(s) 194
Eco130I CCWWGG 1 cut(s) 159
EcoRII CCWGG 1 cut(s) 415
EcoT14I CCWWGG 1 cut(s) 159
ErhI CCWWGG 1 cut(s) 159
FaeI CATG 3 cut(s) 78, 293, 346
FaqI GGGAC 1 cut(s) 221
FatI CATG 3 cut(s) 74, 289, 342
FauNDI CATATG 1 cut(s) 42
Fnu4HI GCNGC 1 cut(s) 547
FokI GGATG 1 cut(s) 29
Fsp4HI GCNGC 1 cut(s) 547
FspBI CTAG 2 cut(s) 329, 395
GluI GCNGC 1 cut(s) 547
HapII CCGG 1 cut(s) 228
Hin1II CATG 3 cut(s) 78, 293, 346
HindIII AAGCTT 1 cut(s) 216
HinfI GANTC 3 cut(s) 204, 323, 498
HpaII CCGG 1 cut(s) 228
HphI GGTGA 1 cut(s) 242
Hpy166II GTNNAC 1 cut(s) 392
Hpy188I TCNGA 1 cut(s) 174
Hpy188III TCNNGA 1 cut(s) 343
Hpy8I GTNNAC 1 cut(s) 392
HpyAV CCTTC 1 cut(s) 393
HpyCH4III ACNGT 5 cut(s) 27, 83, 224, 273, 590
HpyCH4V TGCA 7 cut(s) 46, 350, 360, 383, 425, 437, 549
HpyF10VI GCNNNNNNNGC 1 cut(s) 224
HpyF3I CTNAG 2 cut(s) 171, 452
Hsp92II CATG 3 cut(s) 78, 293, 346
LpnPI CCDG 7 cut(s) 241, 381, 402, 415, 423, 429, 458
Lsp1109I GCAGC 1 cut(s) 533
LweI GCATC 1 cut(s) 520
MaeI CTAG 2 cut(s) 329, 395
MaeIII GTNAC 2 cut(s) 77, 488
MboII GAAGA 2 cut(s) 187, 211
MhlI GDGCHC 1 cut(s) 278
MluCI AATT 1 cut(s) 561
MlyI GAGTC 1 cut(s) 507
MmeI TCCRAC 1 cut(s) 582
MnlI CCTC 5 cut(s) 100, 132, 447, 536, 589
MroXI GAANNNNTTC 1 cut(s) 203
MseI TTAA 4 cut(s) 50, 129, 386, 485
MslI CAYNNNNRTG 2 cut(s) 355, 432
MspI CCGG 1 cut(s) 228
MspR9I CCNGG 1 cut(s) 417
Mva1269I GAATGC 2 cut(s) 352, 439
MvaI CCWGG 1 cut(s) 417
MwoI GCNNNNNNNGC 1 cut(s) 224
NdeI CATATG 1 cut(s) 42
NlaIII CATG 3 cut(s) 78, 293, 346
NlaIV GGNNCC 1 cut(s) 157
PagI TCATGA 1 cut(s) 342
PctI GAATGC 2 cut(s) 352, 439
PdmI GAANNNNTTC 1 cut(s) 203
PfeI GAWTC 2 cut(s) 204, 323
PflMI CCANNNNNTGG 1 cut(s) 508
PkrI GCNGC 1 cut(s) 548
PleI GAGTC 1 cut(s) 506
PpsI GAGTC 1 cut(s) 506
Psp6I CCWGG 1 cut(s) 415
PspGI CCWGG 1 cut(s) 415
PspN4I GGNNCC 1 cut(s) 157
RsaI GTAC 2 cut(s) 393, 450
RsaNI GTAC 2 cut(s) 392, 449
RseI CAYNNNNRTG 2 cut(s) 355, 432
SaqAI TTAA 4 cut(s) 50, 129, 386, 485
SatI GCNGC 1 cut(s) 547
SchI GAGTC 1 cut(s) 507
ScrFI CCNGG 1 cut(s) 417
SduI GDGCHC 1 cut(s) 278
SetI ASST 7 cut(s) 38, 161, 220, 370, 404, 450, 458
SfaNI GCATC 1 cut(s) 520
SmiMI CAYNNNNRTG 2 cut(s) 355, 432
Sse9I AATT 1 cut(s) 561
SspMI CTAG 2 cut(s) 329, 395
StyD4I CCNGG 1 cut(s) 415
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 5 cut(s) 27, 83, 224, 273, 590
TaqI TCGA 2 cut(s) 298, 496
TasI AATT 1 cut(s) 561
TatI WGTACW 1 cut(s) 391
TfiI GAWTC 2 cut(s) 204, 323
Tru1I TTAA 4 cut(s) 50, 129, 386, 485
Tru9I TTAA 4 cut(s) 50, 129, 386, 485
TscAI CASTG 4 cut(s) 229, 278, 343, 434
TseI GCWGC 1 cut(s) 546
TspDTI ATGAA 3 cut(s) 107, 336, 359
TspRI CASTG 4 cut(s) 229, 278, 343, 434
Van91I CCANNNNNTGG 1 cut(s) 508
XapI RAATTY 1 cut(s) 561
XmnI GAANNNNTTC 1 cut(s) 203
XspI CTAG 2 cut(s) 329, 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.