Rroxscaffold_4G00301360

Reverse transcriptase (RNA-dependent DNA polymerase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001006
Physical Location & Seq
Reverse (-)
21743259 .. 21744112
854 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00301360.1

Sequence Viewer

Length: 204 bp
ATGGCCTTAGTTGCTCATTATGACATGGAGCTGCACCAAATGGATGTCAAGACAGCTTTCTTGAATGGTGAGCTTGAGGAAGAGATTTACATGAAGCAACCCGAGGGCTTCATTGAACCTGGAACTGAAAAGTTAGTGTGTAAGTTGAATAGATCCATCTATGGTTTGAAACGAGGCTTCAAGACAGTGGTACTTGAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS RVT_2

Protein Analysis

67

Amino Acids

7.73

Weight (kDa)

5.26

Isoelectric Point (pI)

58.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 1 - 58 2.3e-19 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 147
AfaI GTAC 1 cut(s) 192
AgsI TTSAA 6 cut(s) 64, 116, 148, 169, 181, 197
AjnI CCWGG 1 cut(s) 118
AluBI AGCT 3 cut(s) 31, 56, 73
AluI AGCT 3 cut(s) 31, 56, 73
AlwI GGATC 1 cut(s) 147
Ama87I CYCGRG 1 cut(s) 101
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 31
AsuHPI GGTGA 1 cut(s) 80
AvaI CYCGRG 1 cut(s) 101
BbvI GCAGC 1 cut(s) 18
BccI CCATC 1 cut(s) 164
BciT130I CCWGG 1 cut(s) 120
BisI GCNGC 1 cut(s) 32
BlsI GCNGC 1 cut(s) 33
Bme1390I CCNGG 1 cut(s) 120
BmeT110I CYCGRG 1 cut(s) 101
BmrFI CCNGG 1 cut(s) 120
BpuEI CTTGAG 1 cut(s) 95
BsaJI CCNNGG 1 cut(s) 102
BseBI CCWGG 1 cut(s) 120
BseDI CCNNGG 1 cut(s) 102
BseGI GGATG 1 cut(s) 49
BseXI GCAGC 1 cut(s) 18
BsgI GTGCAG 1 cut(s) 17
BshFI GGCC 1 cut(s) 5
BsiHKCI CYCGRG 1 cut(s) 101
BsnI GGCC 1 cut(s) 5
BsoBI CYCGRG 1 cut(s) 101
Bsp143I GATC 1 cut(s) 152
BspANI GGCC 1 cut(s) 5
BspPI GGATC 1 cut(s) 147
BssECI CCNNGG 1 cut(s) 102
BssMI GATC 1 cut(s) 152
Bst2UI CCWGG 1 cut(s) 120
Bst4CI ACNGT 1 cut(s) 187
Bst6I CTCTTC 1 cut(s) 75
BstDEI CTNAG 1 cut(s) 7
BstF5I GGATG 1 cut(s) 49
BstKTI GATC 1 cut(s) 155
BstMBI GATC 1 cut(s) 152
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 1 cut(s) 120
BstSCI CCNGG 1 cut(s) 118
BstV1I GCAGC 1 cut(s) 18
BstX2I RGATCY 1 cut(s) 152
BstYI RGATCY 1 cut(s) 152
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 1 cut(s) 49
BtsIMutI CAGTG 1 cut(s) 192
Csp6I GTAC 1 cut(s) 191
CviAII CATG 2 cut(s) 25, 91
CviJI RGCY 6 cut(s) 5, 31, 56, 73, 108, 177
CviKI_1 RGCY 6 cut(s) 5, 31, 56, 73, 108, 177
CviQI GTAC 1 cut(s) 191
DdeI CTNAG 1 cut(s) 7
DpnI GATC 1 cut(s) 154
DpnII GATC 1 cut(s) 152
Eam1104I CTCTTC 1 cut(s) 75
EarI CTCTTC 1 cut(s) 75
Eco88I CYCGRG 1 cut(s) 101
EcoRII CCWGG 1 cut(s) 118
FaeI CATG 2 cut(s) 28, 94
FaiI YATR 4 cut(s) 21, 26, 92, 162
FatI CATG 2 cut(s) 24, 90
Fnu4HI GCNGC 1 cut(s) 32
FokI GGATG 1 cut(s) 56
Fsp4HI GCNGC 1 cut(s) 32
GluI GCNGC 1 cut(s) 32
HaeIII GGCC 1 cut(s) 5
Hin1II CATG 2 cut(s) 28, 94
HphI GGTGA 1 cut(s) 80
Hpy188III TCNNGA 3 cut(s) 49, 61, 181
HpyCH4III ACNGT 1 cut(s) 187
HpyCH4V TGCA 1 cut(s) 34
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 1 cut(s) 7
Hsp92II CATG 2 cut(s) 28, 94
Kzo9I GATC 1 cut(s) 152
LmnI GCTCC 1 cut(s) 28
LpnPI CCDG 2 cut(s) 105, 132
Lsp1109I GCAGC 1 cut(s) 18
MalI GATC 1 cut(s) 154
MboI GATC 1 cut(s) 152
MboII GAAGA 1 cut(s) 92
MflI RGATCY 1 cut(s) 152
MnlI CCTC 3 cut(s) 70, 97, 167
MspR9I CCNGG 1 cut(s) 120
MvaI CCWGG 1 cut(s) 120
MwoI GCNNNNNNNGC 1 cut(s) 11
NdeII GATC 1 cut(s) 152
NlaIII CATG 2 cut(s) 28, 94
PkrI GCNGC 1 cut(s) 33
Psp6I CCWGG 1 cut(s) 118
PspGI CCWGG 1 cut(s) 118
PsuI RGATCY 1 cut(s) 152
RsaI GTAC 1 cut(s) 192
RsaNI GTAC 1 cut(s) 191
SatI GCNGC 1 cut(s) 32
Sau3AI GATC 1 cut(s) 152
ScrFI CCNGG 1 cut(s) 120
SetI ASST 4 cut(s) 33, 58, 75, 121
SmlI CTYRAG 1 cut(s) 74
SmoI CTYRAG 1 cut(s) 74
StyD4I CCNGG 1 cut(s) 118
TaaI ACNGT 1 cut(s) 187
TscAI CASTG 1 cut(s) 192
TseI GCWGC 1 cut(s) 31
TspDTI ATGAA 2 cut(s) 100, 107
TspRI CASTG 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.