Rw1G022080

domain in FBox and BRCT domain containing plant proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0001006
Physical Location & Seq
Forward (+)
46767648 .. 46768946
1299 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G022080.1

Sequence Viewer

Length: 510 bp
ATGGCCTTAGTAGCTCACTACAACTTGGAGCTGCATCAAATGGATGTCAAGACAGCGTTTTTGAATGGAGAATTAGTGGAGGACATTTATATGATGCAACCACCAGGTTTTGTTGAAAAGGGGAAGGAGCACATGCCTTATGCATCTTTAGTTGGGAGCTTAATGTATGCACAAGTATGCACAAGACCGGACTTGGCTTTTGTTGTGGGAGTTCTAGGAAGGTTTCAATCAAATCCTGGTTTGCATCATTGGACTGCAGCTAAAAAGGTTCTGCCTGCTGTGTTCTTTTCAAAGAACAATAAAAGGACTTCTGCTTCAAGACTCATGGATGTTAAATATCTAGTTGTGAGGGACAAGGTCAAAGACAAAGAAATCACCATTGAGTACATTTCTTCGAAGTCAAACATTGCTGATCCTCTTACCAAGAGTTTACCAGTGGGAGTGTTTCAAAATCATGTTGCTAATATGGGAATACTCGAGTCTTTTGATGCAGCAGATCAGTGGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

169

Amino Acids

18.97

Weight (kDa)

7.02

Isoelectric Point (pI)

39.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 2 - 44 1.1e-10 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 407
AfaI GTAC 1 cut(s) 386
AgsI TTSAA 6 cut(s) 64, 116, 227, 291, 318, 449
AjnI CCWGG 2 cut(s) 103, 235
AluBI AGCT 4 cut(s) 14, 31, 159, 260
AluI AGCT 4 cut(s) 14, 31, 159, 260
Alw21I GWGCWC 1 cut(s) 132
AlwI GGATC 1 cut(s) 407
Ama87I CYCGRG 1 cut(s) 476
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 3 cut(s) 31, 257, 491
ArsI GACNNNNNNTTYG 4 cut(s) 43, 75, 182, 214
AsuHPI GGTGA 1 cut(s) 367
AsuII TTCGAA 1 cut(s) 395
AvaI CYCGRG 1 cut(s) 476
Bbv12I GWGCWC 1 cut(s) 132
BbvI GCAGC 3 cut(s) 18, 269, 503
BciT130I CCWGG 2 cut(s) 105, 237
BfaI CTAG 2 cut(s) 215, 341
BfmI CTRYAG 1 cut(s) 255
BisI GCNGC 3 cut(s) 32, 258, 492
BlsI GCNGC 3 cut(s) 33, 259, 493
Bme1390I CCNGG 2 cut(s) 105, 237
BmeT110I CYCGRG 1 cut(s) 476
BmrFI CCNGG 2 cut(s) 105, 237
BmsI GCATC 5 cut(s) 43, 84, 152, 253, 478
Bpu14I TTCGAA 1 cut(s) 395
BsaWI WCCGGW 1 cut(s) 187
BsaXI ACNNNNNCTCC 2 cut(s) 60, 90
Bse1I ACTGG 1 cut(s) 434
Bse3DI GCAATG 1 cut(s) 405
BseBI CCWGG 2 cut(s) 105, 237
BseGI GGATG 2 cut(s) 49, 334
BseMI GCAATG 1 cut(s) 405
BseNI ACTGG 1 cut(s) 434
BseXI GCAGC 3 cut(s) 18, 269, 503
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 132
BsiHKCI CYCGRG 1 cut(s) 476
BsiSI CCGG 1 cut(s) 188
BslFI GGGAC 1 cut(s) 365
BsmFI GGGAC 1 cut(s) 365
BsnI GGCC 1 cut(s) 5
BsoBI CYCGRG 1 cut(s) 476
Bsp119I TTCGAA 1 cut(s) 395
Bsp1286I GDGCHC 1 cut(s) 132
Bsp143I GATC 2 cut(s) 412, 496
BspANI GGCC 1 cut(s) 5
BspMAI CTGCAG 1 cut(s) 259
BspPI GGATC 1 cut(s) 407
BspT104I TTCGAA 1 cut(s) 395
BsrDI GCAATG 1 cut(s) 405
BsrI ACTGG 1 cut(s) 434
BssMI GATC 2 cut(s) 412, 496
Bst2UI CCWGG 2 cut(s) 105, 237
BstBI TTCGAA 1 cut(s) 395
BstC8I GCNNGC 1 cut(s) 276
BstDEI CTNAG 1 cut(s) 7
BstF5I GGATG 2 cut(s) 49, 334
BstKTI GATC 2 cut(s) 415, 499
BstMBI GATC 2 cut(s) 412, 496
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 2 cut(s) 105, 237
BstNSI RCATGY 1 cut(s) 136
BstSCI CCNGG 2 cut(s) 103, 235
BstSFI CTRYAG 1 cut(s) 255
BstV1I GCAGC 3 cut(s) 18, 269, 503
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 2 cut(s) 49, 334
BtsIMutI CAGTG 2 cut(s) 441, 506
Cac8I GCNNGC 1 cut(s) 276
CsiI ACCWGGT 1 cut(s) 103
Csp6I GTAC 1 cut(s) 385
CviAII CATG 3 cut(s) 133, 325, 455
CviJI RGCY 6 cut(s) 5, 14, 31, 159, 197, 260
CviKI_1 RGCY 6 cut(s) 5, 14, 31, 159, 197, 260
CviQI GTAC 1 cut(s) 385
DdeI CTNAG 1 cut(s) 7
DpnI GATC 2 cut(s) 414, 498
DpnII GATC 2 cut(s) 412, 496
Eco88I CYCGRG 1 cut(s) 476
EcoRII CCWGG 2 cut(s) 103, 235
EcoT22I ATGCAT 1 cut(s) 145
FaeI CATG 3 cut(s) 136, 328, 458
FaiI YATR 9 cut(s) 90, 92, 134, 141, 168, 178, 326, 456, 467
FaqI GGGAC 1 cut(s) 365
FatI CATG 3 cut(s) 132, 324, 454
Fnu4HI GCNGC 3 cut(s) 32, 258, 492
FokI GGATG 2 cut(s) 56, 341
Fsp4HI GCNGC 3 cut(s) 32, 258, 492
FspBI CTAG 2 cut(s) 215, 341
GluI GCNGC 3 cut(s) 32, 258, 492
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 188
Hin1II CATG 3 cut(s) 136, 328, 458
HinfI GANTC 2 cut(s) 321, 479
HpaII CCGG 1 cut(s) 188
HphI GGTGA 1 cut(s) 367
Hpy166II GTNNAC 1 cut(s) 431
Hpy188III TCNNGA 2 cut(s) 49, 318
Hpy8I GTNNAC 1 cut(s) 431
HpyAV CCTTC 2 cut(s) 118, 213
HpyCH4V TGCA 8 cut(s) 34, 97, 143, 170, 180, 244, 257, 491
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 1 cut(s) 7
Hsp92II CATG 3 cut(s) 136, 328, 458
Kzo9I GATC 2 cut(s) 412, 496
LmnI GCTCC 3 cut(s) 28, 127, 156
LpnPI CCDG 7 cut(s) 90, 117, 201, 222, 249, 288, 447
Lsp1109I GCAGC 3 cut(s) 18, 269, 503
LweI GCATC 5 cut(s) 43, 84, 152, 253, 478
MabI ACCWGGT 1 cut(s) 103
MaeI CTAG 2 cut(s) 215, 341
MalI GATC 2 cut(s) 414, 498
MboI GATC 2 cut(s) 412, 496
MboII GAAGA 1 cut(s) 384
MhlI GDGCHC 1 cut(s) 132
MluCI AATT 1 cut(s) 71
MlyI GAGTC 2 cut(s) 315, 488
MnlI CCTC 3 cut(s) 73, 342, 426
Mph1103I ATGCAT 1 cut(s) 145
MseI TTAA 2 cut(s) 161, 333
MslI CAYNNNNRTG 2 cut(s) 89, 175
MspI CCGG 1 cut(s) 188
MspR9I CCNGG 2 cut(s) 105, 237
MvaI CCWGG 2 cut(s) 105, 237
MwoI GCNNNNNNNGC 1 cut(s) 11
NdeII GATC 2 cut(s) 412, 496
NlaIII CATG 3 cut(s) 136, 328, 458
NsiI ATGCAT 1 cut(s) 145
NspI RCATGY 1 cut(s) 136
NspV TTCGAA 1 cut(s) 395
PaeR7I CTCGAG 1 cut(s) 476
PflFI GACNNNGTC 1 cut(s) 356
PkrI GCNGC 3 cut(s) 33, 259, 493
PleI GAGTC 2 cut(s) 315, 487
PpsI GAGTC 2 cut(s) 315, 487
Psp6I CCWGG 2 cut(s) 103, 235
PspGI CCWGG 2 cut(s) 103, 235
PspXI VCTCGAGB 1 cut(s) 476
PstI CTGCAG 1 cut(s) 259
PsyI GACNNNGTC 1 cut(s) 356
RsaI GTAC 1 cut(s) 386
RsaNI GTAC 1 cut(s) 385
RseI CAYNNNNRTG 2 cut(s) 89, 175
SaqAI TTAA 2 cut(s) 161, 333
SatI GCNGC 3 cut(s) 32, 258, 492
Sau3AI GATC 2 cut(s) 412, 496
SchI GAGTC 2 cut(s) 315, 488
ScrFI CCNGG 2 cut(s) 105, 237
SduI GDGCHC 1 cut(s) 132
SetI ASST 8 cut(s) 16, 33, 109, 161, 224, 262, 270, 360
SexAI ACCWGGT 1 cut(s) 103
SfaNI GCATC 5 cut(s) 43, 84, 152, 253, 478
SfcI CTRYAG 1 cut(s) 255
Sfr274I CTCGAG 1 cut(s) 476
SfuI TTCGAA 1 cut(s) 395
SlaI CTCGAG 1 cut(s) 476
SmiMI CAYNNNNRTG 2 cut(s) 89, 175
SmlI CTYRAG 1 cut(s) 476
SmoI CTYRAG 1 cut(s) 476
Sse9I AATT 1 cut(s) 71
SspMI CTAG 2 cut(s) 215, 341
StyD4I CCNGG 2 cut(s) 103, 235
TaqI TCGA 2 cut(s) 395, 477
TasI AATT 1 cut(s) 71
TatI WGTACW 1 cut(s) 384
Tru1I TTAA 2 cut(s) 161, 333
Tru9I TTAA 2 cut(s) 161, 333
TscAI CASTG 2 cut(s) 441, 506
TseI GCWGC 3 cut(s) 31, 257, 491
TspRI CASTG 2 cut(s) 441, 506
Tth111I GACNNNGTC 1 cut(s) 356
XceI RCATGY 1 cut(s) 136
XhoI CTCGAG 1 cut(s) 476
XspI CTAG 2 cut(s) 215, 341
Zsp2I ATGCAT 1 cut(s) 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.