Rw5G013410
ERF Family

DNA RNA polymerases superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001006
Physical Location & Seq
Forward (+)
15754803 .. 15756098
1296 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G013410.1

Sequence Viewer

Length: 576 bp
ATGGCCTTAGTAGCTCACTTTGATCTTGAGCTGCATCAAATGGACGTCAAGACAGCCTTTCTGAATGGAAATCTTGATGAAGAGATATACATGGTGCAGCCCGAGGGTTTCGTTGCTAAGGGACAGACTGACAAGGTATGTAAACTTAAGAAATCAATTTATGGTTTAAAACAGGCTTCTAGACAGTGGTACCTCAAATTTGATTCAGTAATTACTTCATTCGGTTTTGAGGAAAACAAAGTAGATGAATGCATATATATGAAAATCAGTGGGAGCAGGTTCACTTTCTTGATCTTATACGTGGTAGATTCAGTAGAAAGACCGTTAAAGCTTTATTGTGATAATGCAGCTGCAGTGTTTTTTGCTAAGAACAATAAGAGATCCACAGCCTCAAGGAATATAGATGTGAAGTACTATGCAGTCAGAGAGAGTGTAAGGAACAATGAAATTGAGGTAATTAAAATTGGCACTCTAGCTCAGCTTGCTGACCCCCTCACAAAGGCCATTGCAGTAGCACCTTTTCAGGTACATGTCAAAAATATGGGTGTCCTAGAAAGTCTAGACATGGCAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 Retrotrans_gag zf-CCHC

Protein Analysis

191

Amino Acids

21.66

Weight (kDa)

6.84

Isoelectric Point (pI)

44.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 2 - 103 5.7e-30 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 48
Acc36I ACCTGC 1 cut(s) 267
Acc65I GGTACC 1 cut(s) 189
AccB1I GGYRCC 1 cut(s) 189
AclWI GGATC 1 cut(s) 375
AcsI RAATTY 1 cut(s) 197
AcyI GRCGYC 1 cut(s) 45
AfaI GTAC 3 cut(s) 191, 413, 528
AfiI CCNNNNNNNGG 1 cut(s) 499
AflII CTTAAG 1 cut(s) 146
AflIII ACRYGT 1 cut(s) 529
AluBI AGCT 6 cut(s) 14, 31, 331, 350, 476, 481
AluI AGCT 6 cut(s) 14, 31, 331, 350, 476, 481
AlwI GGATC 1 cut(s) 375
Ama87I CYCGRG 1 cut(s) 101
AoxI GGCC 2 cut(s) 3, 501
ApeKI GCWGC 4 cut(s) 31, 97, 347, 350
ApoI RAATTY 1 cut(s) 197
Asp718I GGTACC 1 cut(s) 189
AvaI CYCGRG 1 cut(s) 101
BaeI ACNNNNGTAYC 2 cut(s) 173, 206
BanI GGYRCC 1 cut(s) 189
BbvI GCAGC 4 cut(s) 18, 109, 337, 359
BfaI CTAG 4 cut(s) 180, 473, 551, 560
BfmI CTRYAG 1 cut(s) 351
BfrI CTTAAG 1 cut(s) 146
BfuAI ACCTGC 1 cut(s) 267
BisI GCNGC 4 cut(s) 32, 98, 348, 351
BlpI GCTNAGC 1 cut(s) 477
BlsI GCNGC 4 cut(s) 33, 99, 349, 352
BmcAI AGTACT 1 cut(s) 413
BmeT110I CYCGRG 1 cut(s) 101
BmiI GGNNCC 1 cut(s) 191
BmsI GCATC 1 cut(s) 43
Bpu10I CCTNAGC 1 cut(s) 117
Bpu1102I GCTNAGC 1 cut(s) 477
BpuEI CTTGAG 2 cut(s) 47, 376
BsaAI YACGTR 1 cut(s) 301
BsaHI GRCGYC 1 cut(s) 45
BsaJI CCNNGG 1 cut(s) 102
Bsc4I CCNNNNNNNGG 1 cut(s) 499
Bse3DI GCAATG 1 cut(s) 504
BseDI CCNNGG 1 cut(s) 102
BseLI CCNNNNNNNGG 1 cut(s) 499
BseMI GCAATG 1 cut(s) 504
BseMII CTCAG 1 cut(s) 491
BseXI GCAGC 4 cut(s) 18, 109, 337, 359
BsgI GTGCAG 1 cut(s) 116
BshFI GGCC 2 cut(s) 5, 503
BshNI GGYRCC 1 cut(s) 189
BsiHKCI CYCGRG 1 cut(s) 101
BslFI GGGAC 1 cut(s) 135
BslI CCNNNNNNNGG 1 cut(s) 499
BsmFI GGGAC 1 cut(s) 135
BsmI GAATGC 1 cut(s) 254
BsnI GGCC 2 cut(s) 5, 503
BsoBI CYCGRG 1 cut(s) 101
Bsp143I GATC 3 cut(s) 22, 291, 380
Bsp1720I GCTNAGC 1 cut(s) 477
BspANI GGCC 2 cut(s) 5, 503
BspCNI CTCAG 1 cut(s) 490
BspLI GGNNCC 1 cut(s) 191
BspMAI CTGCAG 1 cut(s) 355
BspMI ACCTGC 1 cut(s) 267
BspPI GGATC 1 cut(s) 375
BspT107I GGYRCC 1 cut(s) 189
BspTI CTTAAG 1 cut(s) 146
BsrDI GCAATG 1 cut(s) 504
BssECI CCNNGG 1 cut(s) 102
BssMI GATC 3 cut(s) 22, 291, 380
BssNI GRCGYC 1 cut(s) 45
Bst4CI ACNGT 2 cut(s) 186, 324
Bst6I CTCTTC 1 cut(s) 75
BstACI GRCGYC 1 cut(s) 45
BstAFI CTTAAG 1 cut(s) 146
BstBAI YACGTR 1 cut(s) 301
BstC8I GCNNGC 1 cut(s) 483
BstDEI CTNAG 4 cut(s) 7, 117, 366, 477
BstENI CCTNNNNNAGG 1 cut(s) 497
BstKTI GATC 3 cut(s) 25, 294, 383
BstMBI GATC 3 cut(s) 22, 291, 380
BstMWI GCNNNNNNNGC 2 cut(s) 11, 482
BstNSI RCATGY 1 cut(s) 533
BstSFI CTRYAG 1 cut(s) 351
BstV1I GCAGC 4 cut(s) 18, 109, 337, 359
BstX2I RGATCY 1 cut(s) 380
BstYI RGATCY 1 cut(s) 380
BsuRI GGCC 2 cut(s) 5, 503
BtsI GCAGTG 1 cut(s) 360
BtsIMutI CAGTG 3 cut(s) 191, 274, 360
BveI ACCTGC 1 cut(s) 267
Cac8I GCNNGC 1 cut(s) 483
Csp6I GTAC 3 cut(s) 190, 412, 527
CviAII CATG 3 cut(s) 91, 530, 565
CviQI GTAC 3 cut(s) 190, 412, 527
DdeI CTNAG 4 cut(s) 7, 117, 366, 477
DpnI GATC 3 cut(s) 24, 293, 382
DpnII GATC 3 cut(s) 22, 291, 380
DraI TTTAAA 1 cut(s) 168
Eam1104I CTCTTC 1 cut(s) 75
EarI CTCTTC 1 cut(s) 75
Eco88I CYCGRG 1 cut(s) 101
EcoNI CCTNNNNNAGG 1 cut(s) 497
EcoT22I ATGCAT 1 cut(s) 254
FaeI CATG 3 cut(s) 94, 533, 568
FalI AAGNNNNNCTT 2 cut(s) 41, 73
FaqI GGGAC 1 cut(s) 135
FatI CATG 3 cut(s) 90, 529, 564
Fnu4HI GCNGC 4 cut(s) 32, 98, 348, 351
Fsp4HI GCNGC 4 cut(s) 32, 98, 348, 351
FspBI CTAG 4 cut(s) 180, 473, 551, 560
GluI GCNGC 4 cut(s) 32, 98, 348, 351
HaeIII GGCC 2 cut(s) 5, 503
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 3 cut(s) 94, 533, 568
HindIII AAGCTT 1 cut(s) 329
HinfI GANTC 2 cut(s) 203, 308
Hpy166II GTNNAC 2 cut(s) 143, 282
Hpy188I TCNGA 2 cut(s) 63, 425
Hpy188III TCNNGA 6 cut(s) 26, 49, 74, 180, 289, 560
Hpy8I GTNNAC 2 cut(s) 143, 282
HpyCH4III ACNGT 2 cut(s) 186, 324
HpyCH4IV ACGT 2 cut(s) 45, 300
HpyCH4V TGCA 7 cut(s) 34, 97, 252, 347, 353, 419, 509
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 482
HpyF3I CTNAG 4 cut(s) 7, 117, 366, 477
HpySE526I ACGT 2 cut(s) 45, 300
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 3 cut(s) 94, 533, 568
KpnI GGTACC 1 cut(s) 193
Kzo9I GATC 3 cut(s) 22, 291, 380
LmnI GCTCC 1 cut(s) 273
LpnPI CCDG 3 cut(s) 158, 262, 509
Lsp1109I GCAGC 4 cut(s) 18, 109, 337, 359
LweI GCATC 1 cut(s) 43
MaeI CTAG 4 cut(s) 180, 473, 551, 560
MaeII ACGT 2 cut(s) 45, 300
MalI GATC 3 cut(s) 24, 293, 382
MboI GATC 3 cut(s) 22, 291, 380
MboII GAAGA 1 cut(s) 92
MflI RGATCY 1 cut(s) 380
MluCI AATT 6 cut(s) 156, 197, 210, 447, 456, 462
MnlI CCTC 6 cut(s) 97, 203, 223, 400, 445, 503
Mph1103I ATGCAT 1 cut(s) 254
MseI TTAA 4 cut(s) 147, 167, 326, 459
MslI CAYNNNNRTG 1 cut(s) 257
MspA1I CMGCKG 1 cut(s) 350
MspCI CTTAAG 1 cut(s) 146
Mva1269I GAATGC 1 cut(s) 254
MwoI GCNNNNNNNGC 2 cut(s) 11, 482
NdeII GATC 3 cut(s) 22, 291, 380
NlaIII CATG 3 cut(s) 94, 533, 568
NlaIV GGNNCC 1 cut(s) 191
NsiI ATGCAT 1 cut(s) 254
NspI RCATGY 1 cut(s) 533
PciI ACATGT 1 cut(s) 529
PctI GAATGC 1 cut(s) 254
PfeI GAWTC 2 cut(s) 203, 308
PkrI GCNGC 4 cut(s) 33, 99, 349, 352
Ppu21I YACGTR 1 cut(s) 301
PscI ACATGT 1 cut(s) 529
PspN4I GGNNCC 1 cut(s) 191
PstI CTGCAG 1 cut(s) 355
PsuI RGATCY 1 cut(s) 380
PvuII CAGCTG 1 cut(s) 350
RsaI GTAC 3 cut(s) 191, 413, 528
RsaNI GTAC 3 cut(s) 190, 412, 527
RseI CAYNNNNRTG 1 cut(s) 257
SaqAI TTAA 4 cut(s) 147, 167, 326, 459
SatI GCNGC 4 cut(s) 32, 98, 348, 351
Sau3AI GATC 3 cut(s) 22, 291, 380
ScaI AGTACT 1 cut(s) 413
SfaNI GCATC 1 cut(s) 43
SfcI CTRYAG 1 cut(s) 351
SmiMI CAYNNNNRTG 1 cut(s) 257
SmlI CTYRAG 3 cut(s) 26, 146, 391
SmoI CTYRAG 3 cut(s) 26, 146, 391
Sse9I AATT 6 cut(s) 156, 197, 210, 447, 456, 462
SspMI CTAG 4 cut(s) 180, 473, 551, 560
TaaI ACNGT 2 cut(s) 186, 324
TaiI ACGT 2 cut(s) 48, 303
TasI AATT 6 cut(s) 156, 197, 210, 447, 456, 462
TatI WGTACW 1 cut(s) 411
TfiI GAWTC 2 cut(s) 203, 308
Tru1I TTAA 4 cut(s) 147, 167, 326, 459
Tru9I TTAA 4 cut(s) 147, 167, 326, 459
TscAI CASTG 3 cut(s) 191, 274, 360
TseI GCWGC 4 cut(s) 31, 97, 347, 350
TspDTI ATGAA 5 cut(s) 93, 207, 261, 275, 459
TspRI CASTG 3 cut(s) 191, 274, 360
Vha464I CTTAAG 1 cut(s) 146
XagI CCTNNNNNAGG 1 cut(s) 497
XapI RAATTY 1 cut(s) 197
XbaI TCTAGA 2 cut(s) 179, 559
XceI RCATGY 1 cut(s) 533
XspI CTAG 4 cut(s) 180, 473, 551, 560
ZraI GACGTC 1 cut(s) 46
ZrmI AGTACT 1 cut(s) 413
Zsp2I ATGCAT 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.