MD16G1138000.v1.1
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000472
Physical Location & Seq
Reverse (-)
10563455 .. 10570773
7319 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1138000.v1.1.491

Sequence Viewer

Length: 4272 bp
ATGGAGCTAACACCAGTTGAAAGAAAGGAGCATTTAGTTATAGAGGAGGAGGATGAGGAAGTTCAGTTGCAGTGGGCTGCAATTGAGAGGCTGCCTACATATAAACGGTGCAGAACCGCGCTTTTTGACTTCGATGGTGGCGTAGGCGGCGGGGGGAAGGAGCATGCAGGGAAGAGGGTGGTTGATGTTACAAAGTTGAAAGCAGATGAGAGGCACCTGTTCGTCGAGAAACTCATAAAGCACATAGAGCATGACAATCTCCGACTTCTGCAGAAACTTAGAGAAAGGATAGACAGAGTGAATGTGAAGTTGCCGACGGTGGAGGTGAGGTACAAGAACTTGTTTGTGGAAGCAGAGTGTGATGTAGTCCAAGGAAAGCCACTCCCAACACTATGGAACTCTCTTCTGAGCTTCTTATCAGTTTTTACAAAGGCAATTTGGTGCAAATCTTCCGGAGCGAAGATGAGCATTCTATCAGATGTCAATGGCATCATCAAACCATCGAGGCTTACTCTCCTTCTTGGTACCCCTGGCTGCGGGAAAACCACCTTGTTATTGGCCCTTGCCGGAAAACTCGATAAATCTCTCAAGGTTGCAGGGGAGGTTTCTTATAATGGTTACAAGCTAGATGAGTTTGTTCCTCAAAAAACGTCAGCTTACATAAGCGAATACGACCTTCACATACCTGAGATGACTGTGAGGGAAACAATCGACTTTTCAGCCCGCTGTCAGGGCGTTGGCAGTAGAGCTGATACAATGATGGAAGTGATCAGAAGGGAAAAAGAAGCTGGAATAGTACCTGATTCCGACATTGACACCTACATGAAGGCAATTTCAGTTGAAAGACAAAAGCGAAATCTGCAAACCGATTATGTTTTGAAGATCCTCGGACTGGATACCTGTAGTGACACAATGGTTGGTGATGCGTTACAAAGAGGGATTTCAGGTGGCCAGAAGAAAAGGCTGACAACAGGAGAAATGATTGTAGGTCCTACAAAAACTCTGTTTATGGATGAAATATCAACTGGATTAGACAGCTCGACGACCTTTCAGATAGTTACCTATCTTCAGCAGTTGGTGCACATCACAGATGCAACTGCATTGGTTTCACTCCTTCAACCAGCCCCTGAGACCTTTGATCTATTTGATGATGTAATATTAATGGCAGAGGGGAAGATAGTTTACCACGGTCCTCGTAGCCAGGCACTTCAGTTTTTCGAAGATTGTGGTTTCAAATGCCCAGCAAGAAAGGGTGCTGCAGATTTCCTTCAAGAGGTAATCTCAAAGAAGGATCAAGCACAATACTGGAAGCATACCAACATCCCCTACGATTATGTTTCAGTGGATCAATTTTCTCAACTTTTCAGAGCAAGTTACCTGGGGAAGAAGTTAAATGATGAGCTTTCGGAACCATACGATAAATCACAGTGCCATGACAAGGCCCTATCATTTACCACTTACTCAGTGAGAAAATGGGAATTGTTCAAAGCTTGTATGGGCCGAGAGCTGCTTCTTATGAAACGAAATTCCTTTGTCTATGTATTCAAAACAGCGCAGCTTACCATCATTGCATTTATTACAATGACGGTATTTATACGAACTCAAATGGCTGTGGATCTGACAAGTGCAAATTTTTTGATGGGCTCATTGTTTTATACCCTCGTTCGACTTATGACCAATGGAGTTGCAGAGCTGTCCTTGACTGTTACTAGACTTCCAGTTGTTTACAAGCAAAGGGGATTCTATCTGTACCCGGCATGGGCGTATTGTATTCCAGCTTCTCTCCTGAAGGTTCCATTTTCACTACTTGATTCAGCACTATGGACAGCCATAACTTACTACGTTATTGGGTTTAGCCCGGAAATAACAAGGTTCTTCTGCCAGTTTCTTATGCTATTTGCTCTGCATCAATCATCAACATCAATGTGTCGTTTGGTCGCTGTAATCTTCCGAACTATGGTTCTTGCATCAACTTGTGGTATTTTCATCTTAGTGGTAATGTTTTTATGTGGAGGCTTCATTTTGCCACAACCCTCTTTACCTTCTTGGTTGAGGTGGGGATTCTGGTGTTCTCCTATGACTTATGCAGAAATAGGTACAACTCTAAACGAATTCCTTGCTCCTCGATGGCAAAAGGTTACAAATGGAAACACAACCTTAGGAAATGAAGTTCTAACCAGACATGGATTGAACTTTGATGGCTATTTCTATTGGATATCAGTTGGAGCACTGTTTGCGTTCACAGTACTTTTCGATCTTGGATTTGTTTTAGCCTTGACTTACCAAAATCCTCCAAAGATGTCTCGCGCAATTATTTCGGGAAAAAGGTTATCCAAATTACAAGGAAAAGATGCTTGCAACATCAGTGAACAGTCGAAAAATGAATCGACTCCAGCTTATTCTTCCCAAACTGTGGGAGGAAAATTCAACTGCGGGAGGATGGTCCTGCCATTTGAGCCACTGACAATATCATTCAAGGATGTGCAGTATTACGTTGCAACCACACCGGAAATGAGAAAGCATGGCTTCAAGCAGAAAAAGCTTCAGCTGCTTAAAGACATTACAGGAGCATTCAGACCGGGAATTCTCACTGCACTGATGGGTGTGAGTGGGGCTGGAAAAACAACTCTCATGGATGTTCTTTCGGGAAGGAAAACTGGAGGTACCATTGAGGGAGATATAAGAATAGGAGGGCATCCGAAGGTCCAGAAGACATTTGCAAGAATATCGGGTTACTGCGAGCAGACTGACATACATTCTCCACTTATCACAGTAGAAGAATCGGTTAGGTACTCCGCTTGGTTGCGGTTGCCACCCGAAACTAATCAAGACTTAAAAGCTAGATTTGTGGAAGAAGTAATCGAAACAATTGAACTGGAGGATATAAGAGATTCTTTAGTTGGCATTCCTGGACAAAGTGGCCTATCAACCGAGCAGCGTAAAAGGCTGACAATTGCAGTGGAGCTTGTTTCCAATCCATCAATAATATTCATGGATGAACCTACATCAGGTTTAGATGCTAGAGCAGCTGCGATTGTCATGCGTGCAGTGAAGAACGTCGTTGCCACAGGAAGAACAACTGTGTGCACAATTCACCAACCAAGCATTGATATCTTCGAGTCTTTCAATGAGTTAATTTTGATGAAAACGGGTGGACAAATCATCTATTCGGGAGCTCTAGGTCATAACTCGAGTGAACTCATCGAATATTTTGAGCGTATTCCTAATGTACCGAAGATCAAAGACAATTACAACCCGGCAACATGGATGTTAGAAGTTACTTCTGCTTCGGTAGAGGCAGAACTCGGTTTGGATTTTGCCAGCATCTATAAAAATTCTACTCAGTATAGAGACTCAATTGATTTGGTAAAACAGTTGAGTGAAGCAAAGCCAGGTTCAAAAGACTTGCACTTTCCCACACATTTTCCCCAAAATAGTTGGGTGCAGTTCAAGGCCTGTCTTTGGAAACAACACTTGTCCTATTGGAGAAGTACCGAATACAATCTAGCGCGTTTCATGTTCATGATCGCTGTATCAGTCATGTTTGGGGTAATCTTCTGGCAGAAAGGGAAGGAAATAAATAATGAGCAGGATTTGATGAACATACTTGGGTCCACGTACATTGCCGTAATATTCCTAGGCGTAACCAATTGCTCAGGAGTTCTGCCTTATATCGCAACTGAGCGCACTGTTTTGTACCGTGAAAGGTTTGCTGGGATGTACTCATCAAAGGCTTATTCATTTGCACAGGTTGTCGTTGAAATACCTTACACAATGTTGCAAGCAATTTTGTATGTGGCCATTACATATCCTACCATAGGGTTCTTTTGGTCACCTACTAAGGTTTTCTGGTACTTATATGCAACATTCTGCACATTTCTATACTTTGTATATCTTGGGATGCTGATAGCTTCTTTGAGCACAAACCTCGACGTAGCTTCCATTCTGGCAACCGCAGTCTACACCTTACTGAATCTTTTCGCTGGCTTCCTCATGCCTGGACCGAAAATTCCAAAGTGGTGGATTTGGTGCTATTGGATATGTCCTACATCATGGTCCTTAAATGGCCTACTGACTTCACAATATGGAGACATGAGCAAAGAAATAGTGATCTTTGAGGAGCATAAAACCGTTGGTTTCTTCCTACAAGATTACTTTGGTTTCCACCATGATCGTTTAGGCCTTGTGGCTCTTGTTCTCATTGCTTTCCCAATTGTTTTTGCTTCTCTATTTGCCTATTGCATTGGGAAATTCAATTTCCAAAGAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1424

Amino Acids

160.65

Weight (kDa)

8.43

Isoelectric Point (pI)

37.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 158 - 339 6.4e-15 ABC transporter
ABC2_membrane PF01061 494 - 705 9.1e-42 ABC-2 type transporter
PDR_assoc PF08370 710 - 772 7e-24 Plant PDR ABC transporter associated
ABC_tran PF00005 851 - 1003 3.3e-20 ABC transporter
ABC2_membrane PF01061 1149 - 1362 7.9e-58 ABC-2 type transporter
ABC2_membrane_3 PF12698 1196 - 1412 3.3e-06 ABC-2 family transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000472)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15230 AT4G15230 AT4G15230 AT4G15230 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15236
fragaria_vesca FvH4_4g21360 FvH4_4g21360 FvH4_4g21390 FvH4_6g49431 FvH4_6g49450
malus_domestica MD09G1044300.v1.1 MD16G1137900.v1.1 MD16G1138000.v1.1
prunus_persica Prupe.1G106800_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.3G273300_v2.0.a1
pyrus_communis pycom111g03460 pycom16g12210
rosa_chinensis RchiOBHm_Chr2g0169331 RchiOBHm_Chr2g0169361 RchiOBHm_Chr2g0169371 RchiOBHm_Chr2g0169381 RchiOBHm_Chr2g0169411 RchiOBHm_Chr4g0427141 RchiOBHm_Chr4g0427321
rosa_laevigata RLG00000007252 RLG00000007258 RLG00000013974 RLG00000021856 RLG00000021857 RLG00000021858 RLG00000021859
rosa_multiflora Rmu_sc0000648.1_g000007 Rmu_sc0000648.1_g000009 Rmu_sc0003813.1_g000016 Rmu_sc0009165.1_g000008 Rmu_sc0022554.1_g000001
rosa_roxburghii Rroxscaffold_2G00082200 Rroxscaffold_2G00082230 Rroxscaffold_5G00368960 Rroxscaffold_5G00369010 Rroxscaffold_7G00200390
rosa_rugosa Rorug02G0539500 Rorug02G0539600 Rorug02G0539700 Rorug04G0216400 Rorug04G0216500 Rorug04G0216500 Rorug04G0216600
rosa_samantha Rh2AG610800 Rh2AG611000 Rh2AG611100 Rh2CG592800 Rh2CG593000 Rh2CG593200 Rh2DG634900 Rh2DG635100 Rh2DG635200 Rh2DG635400 Rh4AG272200 Rh4AG272900 Rh4DG275600 Rh4DG276000
rosa_wichuraiana Rw2G050640 Rw2G050660 Rw2G050670 Rw2G050680 Rw4G023610 Rw4G023680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 612
Acc65I GGTACC 2 cut(s) 524, 2663
AccB1I GGYRCC 3 cut(s) 213, 524, 2663
AccB7I CCANNNNNTGG 1 cut(s) 2413
AccI GTMKAC 1 cut(s) 3960
AccII CGCG 3 cut(s) 119, 2307, 3511
AccIII TCCGGA 1 cut(s) 452
AciI CCGC 9 cut(s) 117, 147, 150, 537, 724, 2433, 2796, 2806, 3954
AclWI GGATC 4 cut(s) 877, 1299, 1353, 1623
AcoI YGGCCR 2 cut(s) 949, 3798
AcsI RAATTY 8 cut(s) 1525, 1630, 2111, 2423, 2583, 3334, 4008, 4250
AcuI CTGAAG 4 cut(s) 1052, 1193, 1808, 2528
AjnI CCWGG 6 cut(s) 529, 1200, 1377, 2908, 3391, 3997
AloI GAACNNNNNNTCC 2 cut(s) 2154, 2186
Alw21I GWGCWC 5 cut(s) 1083, 2230, 3089, 3178, 3923
Alw26I GTCTC 4 cut(s) 1124, 2307, 3345, 4083
Alw44I GTGCAC 2 cut(s) 1079, 3085
AlwI GGATC 4 cut(s) 877, 1299, 1353, 1623
AlwNI CAGNNNCTG 1 cut(s) 1127
Ama87I CYCGRG 1 cut(s) 3190
Aor13HI TCCGGA 1 cut(s) 452
AoxI GGCC 9 cut(s) 558, 949, 1440, 1498, 2920, 3453, 3798, 4066, 4180
ApaLI GTGCAC 2 cut(s) 1079, 3085
ApoI RAATTY 8 cut(s) 1525, 1630, 2111, 2423, 2583, 3334, 4008, 4250
AseI ATTAAT 1 cut(s) 1160
Asp700I GAANNNNTTC 1 cut(s) 3977
Asp718I GGTACC 2 cut(s) 524, 2663
AspA2I CCTAGG 1 cut(s) 3637
AspLEI GCGC 5 cut(s) 121, 1555, 2309, 3511, 3687
AsuC2I CCSGG 4 cut(s) 1754, 1859, 2580, 3257
AsuHPI GGTGA 4 cut(s) 337, 932, 3086, 3825
AsuII TTCGAA 1 cut(s) 1218
AvaI CYCGRG 1 cut(s) 3190
AvaII GGWCC 7 cut(s) 989, 1190, 2443, 2704, 3612, 4001, 4056
AvrII CCTAGG 1 cut(s) 3637
AxyI CCTNAGG 1 cut(s) 2158
BaeGI GKGCMC 2 cut(s) 1083, 3089
BalI TGGCCA 2 cut(s) 951, 3800
BanI GGYRCC 3 cut(s) 213, 524, 2663
BanII GRGCYC 2 cut(s) 1646, 3178
BarI GAAGNNNNNNTAC 4 cut(s) 1166, 1198, 1762, 1794
BbsI GAAGAC 1 cut(s) 2717
Bbv12I GWGCWC 5 cut(s) 1083, 2230, 3089, 3178, 3923
BceAI ACGGC 1 cut(s) 3611
BciT130I CCWGG 6 cut(s) 531, 1202, 1379, 2910, 3393, 3999
BciVI GTATCC 1 cut(s) 889
BclI TGATCA 1 cut(s) 768
BcnI CCSGG 4 cut(s) 1754, 1859, 2580, 3257
BcoDI GTCTC 4 cut(s) 1124, 2307, 3345, 4083
BfaI CTAG 7 cut(s) 626, 1710, 2841, 3021, 3179, 3506, 3638
BfmI CTRYAG 3 cut(s) 269, 901, 1257
BfuI GTATCC 1 cut(s) 889
BlnI CCTAGG 1 cut(s) 3637
BmcAI AGTACT 1 cut(s) 2247
Bme18I GGWCC 7 cut(s) 989, 1190, 2443, 2704, 3612, 4001, 4056
BmeT110I CYCGRG 1 cut(s) 3190
BmiI GGNNCC 6 cut(s) 215, 526, 1410, 1794, 2665, 3613
BpiI GAAGAC 1 cut(s) 2717
BplI GAGNNNNNCTC 4 cut(s) 496, 528, 1265, 1297
BpmI CTGGAG 3 cut(s) 2376, 2679, 2897
Bpu10I CCTNAGC 1 cut(s) 3655
Bpu14I TTCGAA 1 cut(s) 1218
BpuEI CTTGAG 1 cut(s) 572
BpuMI CCSGG 4 cut(s) 1754, 1859, 2580, 3257
BsaAI YACGTR 1 cut(s) 3618
BsaI GGTCTC 1 cut(s) 1124
BsaJI CCNNGG 6 cut(s) 370, 529, 886, 1186, 1378, 3637
BsaWI WCCGGW 2 cut(s) 452, 2506
BsaXI ACNNNNNCTCC 2 cut(s) 314, 344
Bse1I ACTGG 8 cut(s) 14, 897, 1030, 1310, 1718, 1882, 2662, 2880
Bse21I CCTNAGG 1 cut(s) 2158
Bse3DI GCAATG 3 cut(s) 1566, 3621, 4200
BseAI TCCGGA 1 cut(s) 452
BseBI CCWGG 6 cut(s) 531, 1202, 1379, 2910, 3393, 3999
BseDI CCNNGG 6 cut(s) 370, 529, 886, 1186, 1378, 3637
BseMI GCAATG 3 cut(s) 1566, 3621, 4200
BseMII CTCAG 7 cut(s) 398, 678, 1119, 1476, 3356, 3669, 3672
BseNI ACTGG 8 cut(s) 14, 897, 1030, 1310, 1718, 1882, 2662, 2880
BseRI GAGGAG 4 cut(s) 59, 62, 2112, 4133
BseSI GKGCMC 2 cut(s) 1083, 3089
BseYI CCCAGC 2 cut(s) 1240, 3713
BsgI GTGCAG 6 cut(s) 130, 2504, 2577, 3066, 3464, 3856
Bsh1236I CGCG 3 cut(s) 119, 2307, 3511
BshFI GGCC 9 cut(s) 560, 951, 1442, 1500, 2922, 3455, 3800, 4068, 4182
BshNI GGYRCC 3 cut(s) 213, 524, 2663
BsiHKAI GWGCWC 5 cut(s) 1083, 2230, 3089, 3178, 3923
BsiHKCI CYCGRG 1 cut(s) 3190
BsiSI CCGG 7 cut(s) 453, 567, 1754, 1859, 2507, 2579, 3257
BsmAI GTCTC 4 cut(s) 1124, 2307, 3345, 4083
BsmI GAATGC 3 cut(s) 468, 2570, 2904
BsnI GGCC 9 cut(s) 560, 951, 1442, 1500, 2922, 3455, 3800, 4068, 4182
Bso31I GGTCTC 1 cut(s) 1124
BsoBI CYCGRG 1 cut(s) 3190
Bsp119I TTCGAA 1 cut(s) 1218
Bsp1286I GDGCHC 6 cut(s) 1083, 1646, 2230, 3089, 3178, 3923
Bsp13I TCCGGA 1 cut(s) 452
BspACI CCGC 9 cut(s) 117, 147, 150, 537, 724, 2433, 2796, 2806, 3954
BspANI GGCC 9 cut(s) 560, 951, 1442, 1500, 2922, 3455, 3800, 4068, 4182
BspCNI CTCAG 7 cut(s) 399, 679, 1120, 1475, 3355, 3668, 3673
BspEI TCCGGA 1 cut(s) 452
BspFNI CGCG 3 cut(s) 119, 2307, 3511
BspHI TCATGA 1 cut(s) 3522
BspLI GGNNCC 6 cut(s) 215, 526, 1410, 1794, 2665, 3613
BspMAI CTGCAG 2 cut(s) 273, 1261
BspPI GGATC 4 cut(s) 877, 1299, 1353, 1623
BspT104I TTCGAA 1 cut(s) 1218
BspT107I GGYRCC 3 cut(s) 213, 524, 2663
BspTNI GGTCTC 1 cut(s) 1124
BsrDI GCAATG 3 cut(s) 1566, 3621, 4200
BsrI ACTGG 8 cut(s) 14, 897, 1030, 1310, 1718, 1882, 2662, 2880
BssECI CCNNGG 6 cut(s) 370, 529, 886, 1186, 1378, 3637
BssT1I CCWWGG 2 cut(s) 370, 3637
Bst2UI CCWGG 6 cut(s) 531, 1202, 1379, 2910, 3393, 3999
Bst6I CTCTTC 2 cut(s) 167, 408
BstAPI GCANNNNNTGC 2 cut(s) 1078, 2234
BstBAI YACGTR 1 cut(s) 3618
BstBI TTCGAA 1 cut(s) 1218
BstC8I GCNNGC 8 cut(s) 165, 724, 2356, 2741, 3045, 3322, 3783, 3985
BstDSI CCRYGG 1 cut(s) 1186
BstEII GGTNACC 1 cut(s) 3831
BstENI CCTNNNNNAGG 3 cut(s) 1271, 3006, 3816
BstFNI CGCG 3 cut(s) 119, 2307, 3511
BstHHI GCGC 5 cut(s) 121, 1555, 2309, 3511, 3687
BstMAI GTCTC 4 cut(s) 1124, 2307, 3345, 4083
BstNI CCWGG 6 cut(s) 531, 1202, 1379, 2910, 3393, 3999
BstNSI RCATGY 1 cut(s) 167
BstPI GGTNACC 1 cut(s) 3831
BstSFI CTRYAG 3 cut(s) 269, 901, 1257
BstSLI GKGCMC 2 cut(s) 1083, 3089
BstUI CGCG 3 cut(s) 119, 2307, 3511
BstV2I GAAGAC 1 cut(s) 2717
BstX2I RGATCY 2 cut(s) 882, 1615
BstXI CCANNNNNNTGG 2 cut(s) 393, 4020
BstYI RGATCY 2 cut(s) 882, 1615
Bsu36I CCTNAGG 1 cut(s) 2158
BsuI GTATCC 1 cut(s) 889
BsuRI GGCC 9 cut(s) 560, 951, 1442, 1500, 2922, 3455, 3800, 4068, 4182
BtgI CCRYGG 1 cut(s) 1186
BtsI GCAGTG 4 cut(s) 77, 2589, 2964, 3054
Cac8I GCNNGC 8 cut(s) 165, 724, 2356, 2741, 3045, 3322, 3783, 3985
CaiI CAGNNNCTG 1 cut(s) 1127
CciI TCATGA 1 cut(s) 3522
CfoI GCGC 5 cut(s) 121, 1555, 2309, 3511, 3687
CspCI CAANNNNNGTGG 4 cut(s) 2940, 2975, 3604, 3639
EaeI YGGCCR 2 cut(s) 949, 3798
Eam1104I CTCTTC 2 cut(s) 167, 408
EarI CTCTTC 2 cut(s) 167, 408
Ecl136II GAGCTC 1 cut(s) 3176
Eco130I CCWWGG 2 cut(s) 370, 3637
Eco147I AGGCCT 2 cut(s) 3455, 4182
Eco24I GRGCYC 2 cut(s) 1646, 3178
Eco31I GGTCTC 1 cut(s) 1124
Eco32I GATATC 2 cut(s) 2217, 3112
Eco47I GGWCC 7 cut(s) 989, 1190, 2443, 2704, 3612, 4001, 4056
Eco53kI GAGCTC 1 cut(s) 3176
Eco57I CTGAAG 4 cut(s) 1052, 1193, 1808, 2528
Eco81I CCTNAGG 1 cut(s) 2158
Eco88I CYCGRG 1 cut(s) 3190
Eco91I GGTNACC 1 cut(s) 3831
EcoICRI GAGCTC 1 cut(s) 3176
EcoNI CCTNNNNNAGG 3 cut(s) 1271, 3006, 3816
EcoO109I RGGNCCY 2 cut(s) 989, 1441
EcoO65I GGTNACC 1 cut(s) 3831
EcoRI GAATTC 2 cut(s) 2111, 2583
EcoRII CCWGG 6 cut(s) 529, 1200, 1377, 2908, 3391, 3997
EcoRV GATATC 2 cut(s) 2217, 3112
EcoT14I CCWWGG 2 cut(s) 370, 3637
EcoT38I GRGCYC 2 cut(s) 1646, 3178
ErhI CCWWGG 2 cut(s) 370, 3637
FalI AAGNNNNNCTT 4 cut(s) 2510, 2542, 2878, 2910
FauI CCCGC 4 cut(s) 143, 530, 731, 2426
FbaI TGATCA 1 cut(s) 768
FblI GTMKAC 1 cut(s) 3960
FriOI GRGCYC 2 cut(s) 1646, 3178
FspBI CTAG 7 cut(s) 626, 1710, 2841, 3021, 3179, 3506, 3638
GlaI GCGC 5 cut(s) 120, 1554, 2308, 3510, 3686
GsaI CCCAGC 2 cut(s) 1244, 3717
GsuI CTGGAG 3 cut(s) 2376, 2679, 2897
HaeIII GGCC 9 cut(s) 560, 951, 1442, 1500, 2922, 3455, 3800, 4068, 4182
HapII CCGG 7 cut(s) 453, 567, 1754, 1859, 2507, 2579, 3257
HhaI GCGC 5 cut(s) 121, 1555, 2309, 3511, 3687
Hin6I GCGC 5 cut(s) 119, 1553, 2307, 3509, 3685
HinP1I GCGC 5 cut(s) 119, 1553, 2307, 3509, 3685
HindIII AAGCTT 2 cut(s) 1488, 2540
HpaII CCGG 7 cut(s) 453, 567, 1754, 1859, 2507, 2579, 3257
HphI GGTGA 4 cut(s) 337, 932, 3086, 3825
Hpy99I CGWCG 5 cut(s) 227, 319, 1045, 3062, 3935
HpyCH4IV ACGT 6 cut(s) 650, 1842, 2493, 3057, 3617, 3933
HpySE526I ACGT 6 cut(s) 650, 1842, 2493, 3057, 3617, 3933
HspAI GCGC 5 cut(s) 119, 1553, 2307, 3509, 3685
Kpn2I TCCGGA 1 cut(s) 452
KpnI GGTACC 2 cut(s) 528, 2667
Ksp22I TGATCA 1 cut(s) 768
MaeI CTAG 7 cut(s) 626, 1710, 2841, 3021, 3179, 3506, 3638
MaeII ACGT 6 cut(s) 650, 1842, 2493, 3057, 3617, 3933
MfeI CAATTG 6 cut(s) 81, 2868, 2952, 3357, 3649, 4212
MflI RGATCY 2 cut(s) 882, 1615
MhlI GDGCHC 6 cut(s) 1083, 1646, 2230, 3089, 3178, 3923
MlsI TGGCCA 2 cut(s) 951, 3800
MluNI TGGCCA 2 cut(s) 951, 3800
MlyI GAGTC 3 cut(s) 2383, 3128, 3347
MmeI TCCRAC 3 cut(s) 286, 831, 2203
Mox20I TGGCCA 2 cut(s) 951, 3800
MroI TCCGGA 1 cut(s) 452
MroXI GAANNNNTTC 1 cut(s) 3977
MscI TGGCCA 2 cut(s) 951, 3800
MseI TTAA 6 cut(s) 1160, 1391, 2553, 2834, 3134, 4061
MslI CAYNNNNRTG 4 cut(s) 821, 1924, 1991, 3521
Msp20I TGGCCA 2 cut(s) 951, 3800
MspA1I CMGCKG 3 cut(s) 726, 2548, 3029
MspI CCGG 7 cut(s) 453, 567, 1754, 1859, 2507, 2579, 3257
MunI CAATTG 6 cut(s) 81, 2868, 2952, 3357, 3649, 4212
Mva1269I GAATGC 3 cut(s) 468, 2570, 2904
MvaI CCWGG 6 cut(s) 531, 1202, 1379, 2910, 3393, 3999
MvnI CGCG 3 cut(s) 119, 2307, 3511
NciI CCSGG 4 cut(s) 1754, 1859, 2580, 3257
NlaIV GGNNCC 6 cut(s) 215, 526, 1410, 1794, 2665, 3613
NmeAIII GCCGAG 1 cut(s) 1526
NmuCI GTSAC 2 cut(s) 905, 3831
NspI RCATGY 1 cut(s) 167
NspV TTCGAA 1 cut(s) 1218
PaeI GCATGC 1 cut(s) 167
PaeR7I CTCGAG 1 cut(s) 3190
PagI TCATGA 1 cut(s) 3522
PceI AGGCCT 2 cut(s) 3455, 4182
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PctI GAATGC 3 cut(s) 468, 2570, 2904
PdmI GAANNNNTTC 1 cut(s) 3977
PfeI GAWTC 8 cut(s) 803, 1740, 1811, 2061, 2384, 2780, 2891, 3973
PflMI CCANNNNNTGG 1 cut(s) 2413
PfoI TCCNGGA 1 cut(s) 2908
PleI GAGTC 3 cut(s) 2383, 3127, 3347
PpsI GAGTC 3 cut(s) 2383, 3127, 3347
Ppu21I YACGTR 1 cut(s) 3618
PpuMI RGGWCCY 1 cut(s) 989
PshBI ATTAAT 1 cut(s) 1160
PsiI TTATAA 1 cut(s) 612
Psp124BI GAGCTC 1 cut(s) 3178
Psp5II RGGWCCY 1 cut(s) 989
Psp6I CCWGG 6 cut(s) 529, 1200, 1377, 2908, 3391, 3997
PspEI GGTNACC 1 cut(s) 3831
PspFI CCCAGC 2 cut(s) 1240, 3713
PspGI CCWGG 6 cut(s) 529, 1200, 1377, 2908, 3391, 3997
PspN4I GGNNCC 6 cut(s) 215, 526, 1410, 1794, 2665, 3613
PspPPI RGGWCCY 1 cut(s) 989
PspXI VCTCGAGB 1 cut(s) 3190
PstI CTGCAG 2 cut(s) 273, 1261
PstNI CAGNNNCTG 1 cut(s) 1127
PsuI RGATCY 2 cut(s) 882, 1615
PvuII CAGCTG 2 cut(s) 2548, 3029
RseI CAYNNNNRTG 4 cut(s) 821, 1924, 1991, 3521
SacI GAGCTC 1 cut(s) 3178
SaqAI TTAA 6 cut(s) 1160, 1391, 2553, 2834, 3134, 4061
ScaI AGTACT 1 cut(s) 2247
SchI GAGTC 3 cut(s) 2383, 3128, 3347
SduI GDGCHC 6 cut(s) 1083, 1646, 2230, 3089, 3178, 3923
SfcI CTRYAG 3 cut(s) 269, 901, 1257
Sfr274I CTCGAG 1 cut(s) 3190
SfuI TTCGAA 1 cut(s) 1218
SinI GGWCC 7 cut(s) 989, 1190, 2443, 2704, 3612, 4001, 4056
SlaI CTCGAG 1 cut(s) 3190
SmiMI CAYNNNNRTG 4 cut(s) 821, 1924, 1991, 3521
SmlI CTYRAG 2 cut(s) 587, 3190
SmoI CTYRAG 2 cut(s) 587, 3190
SphI GCATGC 1 cut(s) 167
SseBI AGGCCT 2 cut(s) 3455, 4182
SsiI CCGC 9 cut(s) 117, 147, 150, 537, 724, 2433, 2796, 2806, 3954
SspI AATATT 4 cut(s) 1158, 2988, 3209, 3633
SspMI CTAG 7 cut(s) 626, 1710, 2841, 3021, 3179, 3506, 3638
SstI GAGCTC 1 cut(s) 3178
StuI AGGCCT 2 cut(s) 3455, 4182
StyI CCWWGG 2 cut(s) 370, 3637
TaiI ACGT 6 cut(s) 653, 1845, 2496, 3060, 3620, 3936
TaqII GACCGA 1 cut(s) 4018
TatI WGTACW 2 cut(s) 2245, 3720
TauI GCSGC 1 cut(s) 150
TfiI GAWTC 8 cut(s) 803, 1740, 1811, 2061, 2384, 2780, 2891, 3973
Tru1I TTAA 6 cut(s) 1160, 1391, 2553, 2834, 3134, 4061
Tru9I TTAA 6 cut(s) 1160, 1391, 2553, 2834, 3134, 4061
TseFI GTSAC 2 cut(s) 905, 3831
Tsp45I GTSAC 2 cut(s) 905, 3831
Van91I CCANNNNNTGG 1 cut(s) 2413
VneI GTGCAC 2 cut(s) 1079, 3085
VpaK11BI GGWCC 7 cut(s) 989, 1190, 2443, 2704, 3612, 4001, 4056
VspI ATTAAT 1 cut(s) 1160
XagI CCTNNNNNAGG 3 cut(s) 1271, 3006, 3816
XapI RAATTY 8 cut(s) 1525, 1630, 2111, 2423, 2583, 3334, 4008, 4250
XceI RCATGY 1 cut(s) 167
XcmI CCANNNNNNNNNTGG 1 cut(s) 553
XhoI CTCGAG 1 cut(s) 3190
XmaJI CCTAGG 1 cut(s) 3637
XmiI GTMKAC 1 cut(s) 3960
XmnI GAANNNNTTC 1 cut(s) 3977
XspI CTAG 7 cut(s) 626, 1710, 2841, 3021, 3179, 3506, 3638
ZrmI AGTACT 1 cut(s) 2247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.