RLG00000021859

pleiotropic drug resistance protein 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000472
Physical Location & Seq
Forward (+)
78868558 .. 78875580
7023 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021859

Sequence Viewer

Length: 4398 bp
ATGGACTTGGCTGCAGAGATAGGAAGAAGTGTCAGGACCTCGTTTCGTGAGCATGCATCGAGTTTCAGGAGCACTGCAGATGTAAGGTCCATAAAGAAGAATGAAGACGATGAAGTTGAGCTGCAGTGGGCCGCCATTGAGAGGTTGCCAACGTTTGAGCGCATTAGGACTTCATTGTTTGATTGCAGCAAAGATGAGAAGGAAGATAAGGCTAGTAACAAGAGTAGGATGGCACAGATTGATGTGTCGAAGCTAGGATCAATGGAGAGGCATGTGGTCATTCAGAAATTGATCAAGGAAATTGAGGGAGATAACCACCGGCTCTTGCAGAAACTCAGAGAAAGAATAGACAGAGTAGGACTGCAACTGCCGACTGTGGAAGTTCGATACCAGAATTTGTTAGTAGAAGCAGAATGTGAAGTAGTTCAAGGAAAACCTCTCCCAACACTATGGAACACCGTTAAAAGCTTATTCTCTGTTATGACTCAGATAGTTGGGTGTAAGCCTCCAGCATACAACCTAGAAATTCTCAGAGATATCAGTGGCATCATCAAACCATCAAGGATGACTCTATTGCTTGGCCCTCCAGGCTGTGGGAAATCCACTTTATTACAGGCGCTTGCAGGGAAACTTGACCATTCTCTCAAGGTCCGGGGAAATGTAACCTACAATGGTTATAAGCTCAATGAGTTTGTTCCACAGAAGACATCAGCTTATATAAGTCAATATGACTTGCACATTTCTGAAATGACTGTGAGGGAAGCACTGGATTTCGCAGCACGCTGTCAGGGTATTGGAAGCAGAGCAGGTGCCCTATTTTCTTCACATCTTATGGCCATAGTTATATTCCATAAAACTAGATTTAACTTGGAAGCTGTTGCAACAACAGATATAATGAAAGAAGTCACCAGAAGGGAGAAGCAAGAAGGAATTGTCCCGGATTCAGATATTGATACTTATATGAAGGCCATTTCGGTAGAAGGGTTGAAAAAATCTCTCATGACAGACTACATAATAAAGATCCTTGGGCTAGACATGTGTGCTGAGACAATTGTAGGAGATCCAATGCAGAGAGGAATTTCGGGTGGCCAAAAGAAAAGACTAACAACAGGGGAAATGATGATCGGTCCAACAAGAGCTTTCTTTATGGACGAGATATCAACAGGCTTAGATAGTTCCACCACCTTCCAAATCGTCACTTGTCTTCAGCAATTGACACACCTAACAGAATCCACCATCTTGGTTTCACTTCTTCAGCCAACACCTGAGACCTTTCATCTGTTTGATGACATTATCTTGATGGCAGAAGGAAAAGTTGTATACCATGGGCCTAGTGATAATGTGGTAGAATTCTTTGAGAACTGTGGTTTCCGTAGCCCACCACGAAAAGCCATTTCTGACTTCCTCCAAGAGGTGGTTTCCCAAAAGGATCAAGCAAAGTACTGGTACAATGAAGACCAACCCCATAGTTATGTTACTGTCGACAACTTTCTAGATATGTTCAAGGACTTCCATGTTGGAAAGAAGCTTGATAAGGAACTTCAAGAGCCTTTCAAGCGATCCGAGTTCCATAAAGATGCTTTATCATTTAACATTTACTCATTGATAAAGTGGGAACTCTTCAAAGCATGCCTTGCTAGAGAATGGCTTCTGATGAAGCGCAACTCGTTTGTCCATGTATTCAAATCAGCACAGCTTGTGGTCATTGCGCTAGTAACAATGACAGTGTTTCTTCGTACATGGATGAAAGTCGATGAGATCCATTCAAATTTCTTTATGAGTTCTCTGTTCTATGCTCTACTTAGATTGGTGTGCAATGGCACTGCAGAGTTATCATTGACTGCTTCCAGACTTGCTGTGTTTTACAAGCAAAGAGACTTTTATTTCTATCCTGCATGGGCTTATTCGGTTCCATCTGCCATCCTAAAGATTCCATTTTCATTGATAGACGCTTTTCTATGGACAGCACTTACTTATTATGTCATCGGTTACAGTCCTGAACCCGAGAGGTTCTTCAAGCAATTCATTCTTTTATTCCTTGTCCATCAAGTAGCAATATCAATGTTCCGTTTGATTGCATCCTTGGTCCGAAATCCTTCTCTTGCAGCCCCTGTTGGTCTTTTCTCCTTAATTGTAATGTTTCTATTTGGGGGCTTCATAATTCCAAAAACTTCTCTACCTAGCTGGTTGGAATGGGGCTTTTGGCTTTCCCCATTGGTATATGCAGAAATTGGTGCTACAGGAAACGAATTCCATGCTCCAAGGTGGCAAAAGCTCTCATCTTCAAATATCACAATAGGTCAGCAAGCTCTAAAAGACCGTGGTCTTGATTTCAGTGAATACTTCTATTGGATATCTATAGGAGCTTTACTTGGATTTTGGTTGGTTTTCAACCTAGGGTTCACCTGTGCTCTCAGTTATTTAAAGTCTCCAGGGAGTTCCCGAACAATCATTTCTCATGAAAGGTTGTCTCGTCTGAAGGAAAAGGAAAACCTAAACAATTCCCCTCGAAAAAAAAAATTAACTCGTATAGAAACTCGAATAAGAGTTGAAGATTCTACAAATATAGGGGTCTTACCTTTTGAGCCCTTGACTATATCATTTGAGAAAGTGCAATATTTTGTCGACTTACCCCAGAAACTGAGAGAGCAAGGTGTTCCACCAAAACGACTACAACTTCTTCAAGATATAACTGGTGCATTTAGACCTGGAGTTCTTACAGCACTTATGGGCGTTAGTGGAGCTGGAAAGACAACACTGATGGATGTTCTTTCAGGAAGGAAAACTGGTGGAATTATTGAAGGAGACATTAGAATTGGAGGACACCCAAAAGTCCAAGAAACATATGCCAGAATATCTGGGTATTGCGAGCAAAGTGACATACATTCCCCACAGATAACAGTAGAAGAGTCGGTAACGTACTCAGCTTGGTTACGTTTGCCAGCACAGGTTGACAGAACAGTCAGATCTGAATTTGTGAGAGAAGTTCTACAAATGATTGAGCTTGATGAAATCAAGGACGAACTAGTAGGCGTACCTGGTAGGGGTATATCGACTGAACAGCGCAAACGGCTTACTATAGCAGTGGAGCTCGTTTCAAATCCATCCATAATGTTCATGGATGAACCCACCTCTGGTTTAGATGCCAGAGCAGCTGCAATAGTTATGCGAGTGGTCAAAAACATTGTCAGCACTAGGAGGACTGTTGTATGCACCATTCACCAGCCCAGTATTGACATCTTTGAAGCATTCGATGAGCTTATTTTAATGAAAATAGGAGGACAGCTAATCTACAGTGGAGCGCTGGGTCAAAATTCAAGCAAGCTTATTGAATACTTTGAGGGTATTCCTGGGGTTCCAAACATCAAAGAAAATTACAACCCAGCAACATGGATGTTAGAGGTCACCGGTCCTTCTGCAGAAGCTAAACTTGGCTTAGATTTTGCTCAACTCTATAGAGAATCACATCTATGCCGGGAGAACGACGACCTAGTTAGAGAACTCAGCTGTCCAGCAGAAGGGGCAAAGAAACTACATTTTTCTACTCGCTTTGCACAAAATGAATGGGAGCAATTTAAAGCCTGCCTCTGGAAGCAACATCTCTCCTACTGGAGAAATCCACAATATAACCTGGGCAGATTGACTATTACTGCTGCATCCTCTTTGTTATTCGGAGCACTTCTTTGGCAGAAAGGGCAGACAGTAGATAATGAACAGGATTTTTTCAACATTCTGGGATCAATGTTTGTTTTACTGCAATTCATGGGAATAGGAAACTGCTCCTCTGTTTTACCCTGTATTGCTTCAGAGCGGAATGTTGTATACCGAGAAAGGTTTGCTGGAATGTACTCCTCATGGGCCTATTCATTTGCGCAGGTGATCATTGAAATTCCGTACATATTTCTTCAGGCAGTTTTGTTTTCGGCAATCACATATCCAACCATAAATTTCCACTGGACACTTTACCAAGTGTTTTGGTATGTCTATGCCATGTTTTGTACACTGCTCTACTTCAGTTACTTCGGCATATTGCTTGCAGCTTTGACCCCAACATTCCCAGTGGCTATGGTCCTAGCCAGTTTCTGTTACACCATGTTCAACTTATTCTCAGGTTTCCTCGTGCCTGGACCGAAAATTCCTAAATGGTGGGTTTGGGGTTATTGGATTTGCCCTGTAGCATGGTCCTTAAACAGTCTTCTCACTTCACAGTATGGAGATCTAAACAAGGTGATAGTAGTACATGGGGAGGAAAAGACCATCAGTGACTTCTTAGAAAGTATTCATGGGTACTACTATAATGAACTAAAATATGTCGCAATTGTTCTTTTGGCATTTCCACTAGTTTTCGCTTTTGCTTTTGGAGTTGCTACAGAGAAGCTAAACTTCCAAAGGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1466

Amino Acids

166.56

Weight (kDa)

8.02

Isoelectric Point (pI)

38.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 177 - 385 1.5e-13 ABC transporter
ABC2_membrane_7 PF19055 419 - 472 6.8e-06 ABC-2 type transporter
ABC2_membrane PF01061 540 - 751 4.2e-44 ABC-2 type transporter
PDR_assoc PF08370 756 - 818 1.4e-22 Plant PDR ABC transporter associated
ABC_tran PF00005 894 - 1045 3.5e-20 ABC transporter
ABC2_membrane PF01061 1190 - 1404 6.7e-58 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000472)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15230 AT4G15230 AT4G15230 AT4G15230 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15236
fragaria_vesca FvH4_4g21360 FvH4_4g21360 FvH4_4g21390 FvH4_6g49431 FvH4_6g49450
malus_domestica MD09G1044300.v1.1 MD16G1137900.v1.1 MD16G1138000.v1.1
prunus_persica Prupe.1G106800_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.3G273300_v2.0.a1
pyrus_communis pycom111g03460 pycom16g12210
rosa_chinensis RchiOBHm_Chr2g0169331 RchiOBHm_Chr2g0169361 RchiOBHm_Chr2g0169371 RchiOBHm_Chr2g0169381 RchiOBHm_Chr2g0169411 RchiOBHm_Chr4g0427141 RchiOBHm_Chr4g0427321
rosa_laevigata RLG00000007252 RLG00000007258 RLG00000013974 RLG00000021856 RLG00000021857 RLG00000021858 RLG00000021859
rosa_multiflora Rmu_sc0000648.1_g000007 Rmu_sc0000648.1_g000009 Rmu_sc0003813.1_g000016 Rmu_sc0009165.1_g000008 Rmu_sc0022554.1_g000001
rosa_roxburghii Rroxscaffold_2G00082200 Rroxscaffold_2G00082230 Rroxscaffold_5G00368960 Rroxscaffold_5G00369010 Rroxscaffold_7G00200390
rosa_rugosa Rorug02G0539500 Rorug02G0539600 Rorug02G0539700 Rorug04G0216400 Rorug04G0216500 Rorug04G0216500 Rorug04G0216600
rosa_samantha Rh2AG610800 Rh2AG611000 Rh2AG611100 Rh2CG592800 Rh2CG593000 Rh2CG593200 Rh2DG634900 Rh2DG635100 Rh2DG635200 Rh2DG635400 Rh4AG272200 Rh4AG272900 Rh4DG275600 Rh4DG276000
rosa_wichuraiana Rw2G050640 Rw2G050660 Rw2G050670 Rw2G050680 Rw4G023610 Rw4G023680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 678
AarI CACCTGC 2 cut(s) 797, 3865
AasI GACNNNNNNGTC 1 cut(s) 2960
Acc16I TGCGCA 1 cut(s) 3873
Acc36I ACCTGC 2 cut(s) 797, 3865
AccB1I GGYRCC 1 cut(s) 809
AccB7I CCANNNNNTGG 2 cut(s) 593, 1414
AccBSI CCGCTC 1 cut(s) 3811
AccI GTMKAC 4 cut(s) 1320, 1482, 2625, 3822
AciI CCGC 2 cut(s) 132, 3811
AclI AACGTT 1 cut(s) 152
AclWI GGATC 7 cut(s) 265, 1015, 1055, 1437, 1554, 1753, 3745
AcoI YGGCCR 2 cut(s) 834, 1087
AcuI CTGAAG 6 cut(s) 1190, 1238, 2498, 3789, 3890, 3997
AfeI AGCGCT 1 cut(s) 3303
AfiI CCNNNNNNNGG 8 cut(s) 141, 593, 1411, 1414, 3044, 3134, 3518, 3588
AflIII ACRYGT 1 cut(s) 1035
AgeI ACCGGT 1 cut(s) 3407
AhlI ACTAGT 2 cut(s) 3025, 4339
AjnI CCWGG 7 cut(s) 586, 2431, 2707, 3037, 3349, 3630, 4123
AjuI GAANNNNNNNTTGG 2 cut(s) 3414, 3446
Alw21I GWGCWC 4 cut(s) 74, 2413, 3093, 3679
Alw26I GTCTC 6 cut(s) 1040, 1262, 1869, 2433, 2475, 2799
AlwI GGATC 7 cut(s) 265, 1015, 1055, 1437, 1554, 1753, 3745
AlwNI CAGNNNCTG 3 cut(s) 2111, 2641, 4083
Ama87I CYCGRG 1 cut(s) 2003
Aor51HI AGCGCT 1 cut(s) 3303
AoxI GGCC 7 cut(s) 129, 580, 834, 966, 1087, 1328, 3858
ApeKI GCWGC 9 cut(s) 11, 121, 186, 776, 2105, 3152, 3155, 3653, 4037
ArsI GACNNNNNNTTYG 2 cut(s) 3171, 3203
AsiGI ACCGGT 1 cut(s) 3407
Asp700I GAANNNNTTC 4 cut(s) 423, 1647, 2095, 4278
AspA2I CCTAGG 1 cut(s) 2395
AspLEI GCGC 7 cut(s) 162, 619, 1662, 1711, 3066, 3304, 3874
AsuC2I CCSGG 3 cut(s) 653, 938, 3476
AsuHPI GGTGA 6 cut(s) 898, 2395, 3212, 3397, 3889, 4240
AvaI CYCGRG 1 cut(s) 2003
AvaII GGWCC 9 cut(s) 36, 87, 649, 1127, 2086, 3410, 4069, 4127, 4182
AvrII CCTAGG 1 cut(s) 2395
BaeGI GKGCMC 1 cut(s) 814
BalI TGGCCA 2 cut(s) 836, 1089
BanI GGYRCC 1 cut(s) 809
BanII GRGCYC 2 cut(s) 2589, 3093
BauI CACGAG 1 cut(s) 4118
BbsI GAAGAC 5 cut(s) 111, 710, 1196, 1461, 4187
Bbv12I GWGCWC 4 cut(s) 74, 2413, 3093, 3679
BbvI GCAGC 8 cut(s) 108, 198, 788, 2117, 3142, 3164, 3640, 4049
BceAI ACGGC 1 cut(s) 3086
BcgI CGANNNNNNTGC 2 cut(s) 2237, 2271
BciT130I CCWGG 7 cut(s) 588, 2433, 2709, 3039, 3351, 3632, 4125
BclI TGATCA 2 cut(s) 291, 3879
BcnI CCSGG 3 cut(s) 653, 938, 3476
BcoDI GTCTC 6 cut(s) 1040, 1262, 1869, 2433, 2475, 2799
BcuI ACTAGT 2 cut(s) 3025, 4339
BfoI RGCGCY 2 cut(s) 620, 3305
BfuAI ACCTGC 2 cut(s) 797, 3865
BglI GCCNNNNNGGC 1 cut(s) 588
BglII AGATCT 2 cut(s) 2966, 4216
BlnI CCTAGG 1 cut(s) 2395
BmcAI AGTACT 1 cut(s) 1442
Bme18I GGWCC 9 cut(s) 36, 87, 649, 1127, 2086, 3410, 4069, 4127, 4182
BmeT110I CYCGRG 1 cut(s) 2003
BmiI GGNNCC 3 cut(s) 811, 1911, 3357
BmrI ACTGGG 2 cut(s) 3222, 4052
BmsI GCATC 6 cut(s) 65, 555, 1567, 2087, 3133, 3665
BmuI ACTGGG 2 cut(s) 3222, 4052
BoxI GACNNNNGTC 1 cut(s) 2322
BpiI GAAGAC 5 cut(s) 111, 710, 1196, 1461, 4187
BpmI CTGGAG 5 cut(s) 492, 570, 2415, 2730, 3631
BpuEI CTTGAG 1 cut(s) 629
BpuMI CCSGG 3 cut(s) 653, 938, 3476
BsaI GGTCTC 1 cut(s) 1262
BsaWI WCCGGW 1 cut(s) 3407
Bsc4I CCNNNNNNNGG 8 cut(s) 141, 593, 1411, 1414, 3044, 3134, 3518, 3588
Bse118I RCCGGY 2 cut(s) 318, 3407
Bse1I ACTGG 9 cut(s) 771, 1448, 2698, 2791, 3228, 3614, 3959, 4058, 4077
Bse3DI GCAATG 2 cut(s) 1704, 1822
BseBI CCWGG 7 cut(s) 588, 2433, 2709, 3039, 3351, 3632, 4125
BseLI CCNNNNNNNGG 8 cut(s) 141, 593, 1411, 1414, 3044, 3134, 3518, 3588
BseMI GCAATG 2 cut(s) 1704, 1822
BseNI ACTGG 9 cut(s) 771, 1448, 2698, 2791, 3228, 3614, 3959, 4058, 4077
BseRI GAGGAG 2 cut(s) 3772, 3841
BseSI GKGCMC 1 cut(s) 814
BseXI GCAGC 8 cut(s) 108, 198, 788, 2117, 3142, 3164, 3640, 4049
BseYI CCCAGC 2 cut(s) 3304, 3382
BshFI GGCC 7 cut(s) 131, 582, 836, 968, 1089, 1330, 3860
BshNI GGYRCC 1 cut(s) 809
BshTI ACCGGT 1 cut(s) 3407
BsiHKAI GWGCWC 4 cut(s) 74, 2413, 3093, 3679
BsiHKCI CYCGRG 1 cut(s) 2003
BsiSI CCGG 5 cut(s) 319, 652, 938, 3408, 3475
BslFI GGGAC 1 cut(s) 920
BslI CCNNNNNNNGG 8 cut(s) 141, 593, 1411, 1414, 3044, 3134, 3518, 3588
BsmAI GTCTC 6 cut(s) 1040, 1262, 1869, 2433, 2475, 2799
BsmFI GGGAC 1 cut(s) 920
BsmI GAATGC 1 cut(s) 3248
BsnI GGCC 7 cut(s) 131, 582, 836, 968, 1089, 1330, 3860
Bso31I GGTCTC 1 cut(s) 1262
BsoBI CYCGRG 1 cut(s) 2003
Bsp1286I GDGCHC 6 cut(s) 74, 814, 2413, 2589, 3093, 3679
Bsp1407I TGTACA 1 cut(s) 3998
Bsp19I CCATGG 1 cut(s) 1324
BspACI CCGC 2 cut(s) 132, 3811
BspANI GGCC 7 cut(s) 131, 582, 836, 968, 1089, 1330, 3860
BspHI TCATGA 2 cut(s) 999, 2458
BspLI GGNNCC 3 cut(s) 811, 1911, 3357
BspMAI CTGCAG 5 cut(s) 16, 79, 126, 1828, 3421
BspMI ACCTGC 2 cut(s) 797, 3865
BspPI GGATC 7 cut(s) 265, 1015, 1055, 1437, 1554, 1753, 3745
BspT107I GGYRCC 1 cut(s) 809
BspTNI GGTCTC 1 cut(s) 1262
BsrBI CCGCTC 1 cut(s) 3811
BsrDI GCAATG 2 cut(s) 1704, 1822
BsrFI RCCGGY 2 cut(s) 318, 3407
BsrGI TGTACA 1 cut(s) 3998
BsrI ACTGG 9 cut(s) 771, 1448, 2698, 2791, 3228, 3614, 3959, 4058, 4077
BssAI RCCGGY 2 cut(s) 318, 3407
BssNAI GTATAC 2 cut(s) 1321, 3823
BssSI CACGAG 1 cut(s) 4118
BssT1I CCWWGG 5 cut(s) 1024, 1324, 2082, 2261, 2395
Bst1107I GTATAC 2 cut(s) 1321, 3823
Bst2BI CACGAG 1 cut(s) 4118
Bst2UI CCWGG 7 cut(s) 588, 2433, 2709, 3039, 3351, 3632, 4125
Bst6I CTCTTC 2 cut(s) 1625, 2901
BstAPI GCANNNNNTGC 1 cut(s) 1634
BstAUI TGTACA 1 cut(s) 3998
BstDSI CCRYGG 2 cut(s) 1324, 2320
BstEII GGTNACC 1 cut(s) 3403
BstENI CCTNNNNNAGG 1 cut(s) 1409
BstH2I RGCGCY 2 cut(s) 620, 3305
BstHHI GCGC 7 cut(s) 162, 619, 1662, 1711, 3066, 3304, 3874
BstMAI GTCTC 6 cut(s) 1040, 1262, 1869, 2433, 2475, 2799
BstMWI GCNNNNNNNGC 7 cut(s) 588, 1555, 1634, 3070, 3152, 3521, 3694
BstNI CCWGG 7 cut(s) 588, 2433, 2709, 3039, 3351, 3632, 4125
BstNSI RCATGY 4 cut(s) 56, 275, 1039, 1632
BstPAI GACNNNNGTC 1 cut(s) 2322
BstPI GGTNACC 1 cut(s) 3403
BstSLI GKGCMC 1 cut(s) 814
BstV1I GCAGC 8 cut(s) 108, 198, 788, 2117, 3142, 3164, 3640, 4049
BstV2I GAAGAC 5 cut(s) 111, 710, 1196, 1461, 4187
BstX2I RGATCY 5 cut(s) 1020, 1060, 1758, 2966, 4216
BstXI CCANNNNNNTGG 3 cut(s) 450, 1240, 3390
BstYI RGATCY 5 cut(s) 1020, 1060, 1758, 2966, 4216
BstZ17I GTATAC 2 cut(s) 1321, 3823
BsuRI GGCC 7 cut(s) 131, 582, 836, 968, 1089, 1330, 3860
BtgI CCRYGG 2 cut(s) 1324, 2320
BtsI GCAGTG 5 cut(s) 72, 131, 1821, 3090, 4001
BveI ACCTGC 2 cut(s) 797, 3865
CaiI CAGNNNCTG 3 cut(s) 2111, 2641, 4083
CciI TCATGA 2 cut(s) 999, 2458
CfoI GCGC 7 cut(s) 162, 619, 1662, 1711, 3066, 3304, 3874
Cfr10I RCCGGY 2 cut(s) 318, 3407
CseI GACGC 1 cut(s) 1958
CsiI ACCWGGT 1 cut(s) 3037
CspAI ACCGGT 1 cut(s) 3407
DraI TTTAAA 2 cut(s) 2424, 3577
DrdI GACNNNNNNGTC 1 cut(s) 2960
DseDI GACNNNNNNGTC 1 cut(s) 2960
EaeI YGGCCR 2 cut(s) 834, 1087
Eam1104I CTCTTC 2 cut(s) 1625, 2901
EarI CTCTTC 2 cut(s) 1625, 2901
Ecl136II GAGCTC 1 cut(s) 3091
Eco130I CCWWGG 5 cut(s) 1024, 1324, 2082, 2261, 2395
Eco24I GRGCYC 2 cut(s) 2589, 3093
Eco31I GGTCTC 1 cut(s) 1262
Eco32I GATATC 3 cut(s) 538, 1158, 2355
Eco47I GGWCC 9 cut(s) 36, 87, 649, 1127, 2086, 3410, 4069, 4127, 4182
Eco47III AGCGCT 1 cut(s) 3303
Eco53kI GAGCTC 1 cut(s) 3091
Eco57I CTGAAG 6 cut(s) 1190, 1238, 2498, 3789, 3890, 3997
Eco88I CYCGRG 1 cut(s) 2003
Eco91I GGTNACC 1 cut(s) 3403
EcoICRI GAGCTC 1 cut(s) 3091
EcoNI CCTNNNNNAGG 1 cut(s) 1409
EcoO109I RGGNCCY 1 cut(s) 36
EcoO65I GGTNACC 1 cut(s) 3403
EcoRI GAATTC 2 cut(s) 1349, 2249
EcoRII CCWGG 7 cut(s) 586, 2431, 2707, 3037, 3349, 3630, 4123
EcoRV GATATC 3 cut(s) 538, 1158, 2355
EcoT14I CCWWGG 5 cut(s) 1024, 1324, 2082, 2261, 2395
EcoT22I ATGCAT 1 cut(s) 58
EcoT38I GRGCYC 2 cut(s) 2589, 3093
ErhI CCWWGG 5 cut(s) 1024, 1324, 2082, 2261, 2395
FalI AAGNNNNNCTT 5 cut(s) 1617, 1649, 3414, 3446, 4367
FaqI GGGAC 1 cut(s) 920
FauNDI CATATG 1 cut(s) 2845
FbaI TGATCA 2 cut(s) 291, 3879
FblI GTMKAC 4 cut(s) 1320, 1482, 2625, 3822
FriOI GRGCYC 2 cut(s) 2589, 3093
FspI TGCGCA 1 cut(s) 3873
GlaI GCGC 7 cut(s) 161, 618, 1661, 1710, 3065, 3303, 3873
GsaI CCCAGC 2 cut(s) 3308, 3386
GsuI CTGGAG 5 cut(s) 492, 570, 2415, 2730, 3631
HaeII RGCGCY 2 cut(s) 620, 3305
HaeIII GGCC 7 cut(s) 131, 582, 836, 968, 1089, 1330, 3860
HapII CCGG 5 cut(s) 319, 652, 938, 3408, 3475
HgaI GACGC 1 cut(s) 1958
HhaI GCGC 7 cut(s) 162, 619, 1662, 1711, 3066, 3304, 3874
Hin6I GCGC 7 cut(s) 160, 617, 1660, 1709, 3064, 3302, 3872
HinP1I GCGC 7 cut(s) 160, 617, 1660, 1709, 3064, 3302, 3872
HincII GTYRAC 3 cut(s) 1483, 2626, 2953
HindII GTYRAC 3 cut(s) 1483, 2626, 2953
HindIII AAGCTT 3 cut(s) 466, 1526, 3323
HinfI GANTC 8 cut(s) 484, 568, 941, 1229, 1930, 2555, 2909, 3461
HpaII CCGG 5 cut(s) 319, 652, 938, 3408, 3475
HphI GGTGA 6 cut(s) 898, 2395, 3212, 3397, 3889, 4240
Hpy166II GTNNAC 7 cut(s) 1321, 1483, 2403, 2626, 2953, 3823, 4001
Hpy8I GTNNAC 7 cut(s) 1321, 1483, 2403, 2626, 2953, 3823, 4001
Hpy99I CGWCG 1 cut(s) 3488
HpyCH4IV ACGT 3 cut(s) 152, 2918, 2935
HpyF10VI GCNNNNNNNGC 7 cut(s) 588, 1555, 1634, 3070, 3152, 3521, 3694
HpySE526I ACGT 3 cut(s) 152, 2918, 2935
HspAI GCGC 7 cut(s) 160, 617, 1660, 1709, 3064, 3302, 3872
Ksp22I TGATCA 2 cut(s) 291, 3879
LmnI GCTCC 9 cut(s) 69, 2263, 2363, 2741, 3088, 3299, 3568, 3674, 3785
Lsp1109I GCAGC 8 cut(s) 108, 198, 788, 2117, 3142, 3164, 3640, 4049
LweI GCATC 6 cut(s) 65, 555, 1567, 2087, 3133, 3665
MabI ACCWGGT 1 cut(s) 3037
MaeII ACGT 3 cut(s) 152, 2918, 2935
MbiI CCGCTC 1 cut(s) 3811
MfeI CAATTG 3 cut(s) 1050, 1211, 4317
MflI RGATCY 5 cut(s) 1020, 1060, 1758, 2966, 4216
MhlI GDGCHC 6 cut(s) 74, 814, 2413, 2589, 3093, 3679
MlsI TGGCCA 2 cut(s) 836, 1089
MluNI TGGCCA 2 cut(s) 836, 1089
MlyI GAGTC 3 cut(s) 478, 562, 2918
MmeI TCCRAC 4 cut(s) 1154, 1498, 2169, 3962
Mox20I TGGCCA 2 cut(s) 836, 1089
Mph1103I ATGCAT 1 cut(s) 58
MroXI GAANNNNTTC 4 cut(s) 423, 1647, 2095, 4278
MscI TGGCCA 2 cut(s) 836, 1089
MseI TTAA 9 cut(s) 462, 864, 1590, 2129, 2423, 2522, 3266, 3576, 4187
MslI CAYNNNNRTG 3 cut(s) 1470, 1575, 3469
Msp20I TGGCCA 2 cut(s) 836, 1089
MspA1I CMGCKG 2 cut(s) 3155, 3507
MspI CCGG 5 cut(s) 319, 652, 938, 3408, 3475
MunI CAATTG 3 cut(s) 1050, 1211, 4317
Mva1269I GAATGC 1 cut(s) 3248
MvaI CCWGG 7 cut(s) 588, 2433, 2709, 3039, 3351, 3632, 4125
MwoI GCNNNNNNNGC 7 cut(s) 588, 1555, 1634, 3070, 3152, 3521, 3694
NciI CCSGG 3 cut(s) 653, 938, 3476
NcoI CCATGG 1 cut(s) 1324
NdeI CATATG 1 cut(s) 2845
NlaIV GGNNCC 3 cut(s) 811, 1911, 3357
NmuCI GTSAC 5 cut(s) 904, 1195, 2876, 3403, 4262
NsbI TGCGCA 1 cut(s) 3873
NsiI ATGCAT 1 cut(s) 58
NspI RCATGY 4 cut(s) 56, 275, 1039, 1632
PaeI GCATGC 2 cut(s) 56, 1632
PagI TCATGA 2 cut(s) 999, 2458
PaqCI CACCTGC 2 cut(s) 797, 3865
PciI ACATGT 1 cut(s) 1035
PctI GAATGC 1 cut(s) 3248
PdmI GAANNNNTTC 4 cut(s) 423, 1647, 2095, 4278
PfeI GAWTC 5 cut(s) 941, 1229, 1930, 2555, 3461
PflMI CCANNNNNTGG 2 cut(s) 593, 1414
PfoI TCCNGGA 1 cut(s) 936
PinAI ACCGGT 1 cut(s) 3407
PleI GAGTC 3 cut(s) 478, 562, 2917
PpsI GAGTC 3 cut(s) 478, 562, 2917
PpuMI RGGWCCY 1 cut(s) 36
PscI ACATGT 1 cut(s) 1035
PshAI GACNNNNGTC 1 cut(s) 2322
PsiI TTATAA 1 cut(s) 678
Psp124BI GAGCTC 1 cut(s) 3093
Psp1406I AACGTT 1 cut(s) 152
Psp5II RGGWCCY 1 cut(s) 36
Psp6I CCWGG 7 cut(s) 586, 2431, 2707, 3037, 3349, 3630, 4123
PspEI GGTNACC 1 cut(s) 3403
PspFI CCCAGC 2 cut(s) 3304, 3382
PspGI CCWGG 7 cut(s) 586, 2431, 2707, 3037, 3349, 3630, 4123
PspN4I GGNNCC 3 cut(s) 811, 1911, 3357
PspPPI RGGWCCY 1 cut(s) 36
PstI CTGCAG 5 cut(s) 16, 79, 126, 1828, 3421
PstNI CAGNNNCTG 3 cut(s) 2111, 2641, 4083
PsuI RGATCY 5 cut(s) 1020, 1060, 1758, 2966, 4216
PvuII CAGCTG 2 cut(s) 3155, 3507
RseI CAYNNNNRTG 3 cut(s) 1470, 1575, 3469
SacI GAGCTC 1 cut(s) 3093
SalI GTCGAC 2 cut(s) 1481, 2624
SaqAI TTAA 9 cut(s) 462, 864, 1590, 2129, 2423, 2522, 3266, 3576, 4187
ScaI AGTACT 1 cut(s) 1442
SchI GAGTC 3 cut(s) 478, 562, 2918
SduI GDGCHC 6 cut(s) 74, 814, 2413, 2589, 3093, 3679
SexAI ACCWGGT 1 cut(s) 3037
SfaNI GCATC 6 cut(s) 65, 555, 1567, 2087, 3133, 3665
SinI GGWCC 9 cut(s) 36, 87, 649, 1127, 2086, 3410, 4069, 4127, 4182
SmiMI CAYNNNNRTG 3 cut(s) 1470, 1575, 3469
SmlI CTYRAG 1 cut(s) 644
SmoI CTYRAG 1 cut(s) 644
SpeI ACTAGT 2 cut(s) 3025, 4339
SphI GCATGC 2 cut(s) 56, 1632
SsiI CCGC 2 cut(s) 132, 3811
SspI AATATT 1 cut(s) 2618
SstI GAGCTC 1 cut(s) 3093
StyI CCWWGG 5 cut(s) 1024, 1324, 2082, 2261, 2395
TaiI ACGT 3 cut(s) 155, 2921, 2938
TaqII GACCGA 2 cut(s) 1115, 4144
TatI WGTACW 4 cut(s) 1440, 3846, 3998, 4237
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 5 cut(s) 941, 1229, 1930, 2555, 3461
Tru1I TTAA 9 cut(s) 462, 864, 1590, 2129, 2423, 2522, 3266, 3576, 4187
Tru9I TTAA 9 cut(s) 462, 864, 1590, 2129, 2423, 2522, 3266, 3576, 4187
TseFI GTSAC 5 cut(s) 904, 1195, 2876, 3403, 4262
TseI GCWGC 9 cut(s) 11, 121, 186, 776, 2105, 3152, 3155, 3653, 4037
Tsp45I GTSAC 5 cut(s) 904, 1195, 2876, 3403, 4262
TspGWI ACGGA 3 cut(s) 1361, 2057, 3882
Van91I CCANNNNNTGG 2 cut(s) 593, 1414
VpaK11BI GGWCC 9 cut(s) 36, 87, 649, 1127, 2086, 3410, 4069, 4127, 4182
XagI CCTNNNNNAGG 1 cut(s) 1409
XbaI TCTAGA 1 cut(s) 1492
XceI RCATGY 4 cut(s) 56, 275, 1039, 1632
XcmI CCANNNNNNNNNTGG 1 cut(s) 3115
XmaJI CCTAGG 1 cut(s) 2395
XmiI GTMKAC 4 cut(s) 1320, 1482, 2625, 3822
XmnI GAANNNNTTC 4 cut(s) 423, 1647, 2095, 4278
ZrmI AGTACT 1 cut(s) 1442
Zsp2I ATGCAT 1 cut(s) 58
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.