Rw2G050670

pleiotropic drug resistance protein 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000472
Physical Location & Seq
Forward (+)
78299831 .. 78307255
7425 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G050670.1

Sequence Viewer

Length: 4314 bp
ATGGATTTGGCTGCAGAGATAGGAAGAAGTGTGAAGACTTCTTTTCATCAGCATGCATCAAGTTTCAGGAGCACTTCAGATGTCAGGTCCATGAAAGAGAATGATGACGATGAAGTTGAGCTGCAGTGGGCTGCCATTGAAAGATTGCCCACATTTGAACGTATTCGAACTTCACTCATGGATTGTAACAAGGAGGACGATGATCATGAAGGTAAGAGTAAGAGGGTAGTTGATGTTACAAAGCTAGAAGCCCTGGAACGTCATGTGTTCATTGATAAACTTCTCAAGGAAATAGAAGGAGATAATCAACGCCTCTTACAGAAACTCAAAGAAAGAATAGAAAGAGTGGGACTGCAATTGCCAACTGTGGAAGTACGATACCAAAATTTGTTTGTAGAAGCAGAATGTGAAGTAGTTCAAGGAAAGCCCCTTCCAACACTATGGAACACGCTCAAAAGCTCTCTCTCTGTCATGACACAGATAATTGGATGCAAGTCTGAAGCATACAATCTAAAAATTCTCAATGATGTCAGCGGTATCATCAAGCCTTCAAGGATGACTCTTTTACTTGGTCCTCCAGGCTGTGGAAAAACAACCTTATTACAGGCACTTGCAGGAAAACATAACCAATCTCTCAAGGTTCAAGGAGAAATAAACTACAATGGTTATAAGCTCAATGAGTTTGTTCCTCAGAAGACATCAGCTTACATAAGTCAATATGACTTGCATATTTCTGAGATGACTGTGAGGGAAGCACTGGATTTCTCATCTCGTTGCCAGGGCATTGGAAGCAGAGCTGATATTATGAAAGAAGTTGCTCGAAGGGAGAAGCAAGCTGGTATAGTCCCGGAACCAGACATTGACACTTATATGAAGGCCATTTCAATTGAAGGGTTGAAAAAATCTCTAATGACAGACTATATAATAAAGATCCTTGGGCTGGACATTTGTGCTGATACAATCGCAGGGGACGCAATGCAAAGAGGAATTTCTGGTGGTCAAAAGAAAAGGCTTACAACAGGTGAGATGATGATTGGCCCAGCAAGAGCTTTCTTTATGGATGAGATATCAACAGGCCTAGACAGCTCCACTACATATCAAATTGTAACTTGTCTGCAGCAATTGACTCATGTAACAGAATCCACCATTTTGGTTTCACTTCTTCAGCCAACTCCGGAGACTTTTCATCTGTTTGATGACATTATCTTGATGGCTGAAGGAAAAGTTGTATACCATGGGCCAAGGGACAATGTTGTGGAGTTCTTTCAGAAATGCGGTTTTTGTAGCCCACCACGAAAAGGCATTTCTGACTTCCTCCAAGAGGTGGTTTCTCAAAAGGATCAAGCAAAGTACTCGTACCATAAAGACCAACCCTACAGTTATGTTAGTATTGATAAGTTCGTAAATATGTTTAAGGACTTCCATGTCGGAAAGAAGCTTGAAGAGGAGCTTTGCAAGCCTTTTGATCAATCTGTGTGCCATAAACATGCTTTGTCATTCAACATTTACTCACTGAGAAAATGGGAACTATTCAAAGCATGCCTTTCTAGAGAATGGCTTCTCATGAAGAGAAACTCATTTATTCATGTATTCAAATCAGCACAGCTTGTGGTCATTGCACTAGTAACAATGACAATGTTTCTACGTACATGGATGAAAGTCGACGAGATCCATGCAAAGTACTACATGGGTTCTTTGTTTTATGCTCTACTTAGACTGCTATGCAATGGAACTGCAGAGATATCAATGACTGCCTCTAGACTAGCAGTCTTCTATAAGCAAAGAGATATCTACTTCTACCCAGCATGGGCTTATTCTATTCCGGCTGCCATCCTGAAAATTCCATTTTCATTGCTAGATGCATTTCTTTGGACAGCCCTTACTTATTATGTGATTGGTTACAGTCCTGAACCTGAAAGGTTCTTCAGGCAATTTATTCTTCTGTTCCTCGTGCATCAAGTAGCAATATCACTATTTCGTTTGATTGCATCCTTGGTCCGAAATCCTTCTGTTGCAGGATCTGTCAGTCTTTTATCTTTAATAGTAATGTTTCTATTTGGAGGCTTCATAATTCCCAAATCTTCTCTACCTCCTTGGTTGGAATGGGGATTTTGGGTTTCTCCGTTGGCATTTGCAGAAATAGGTGCTTCAATAAATGAATTTCATGCTCCAAGGTGGCAAAAGGTTTTATCTTCCAATGTTACTATAGGCCAGCAAGCTTTAATTAACCGGGGTCTAAACTTCAGTGAATACTTCTATTGGATATCAATAGGAGCTTTATTTGGATTTTGGATGGTTTTCAACCTTGGGTTCACATGCGTACTCAGTTATTCAAAGGCTCCAGGGAGTTCTCGGACTATCATTTCTCATGAAAGATTCTCCCGTCTAAAGAATAAAGAAATTTTAGATAATTCCTGTCAAGAAAAAGAAGTAACTAGTGTAGACACTGACATTATTACTTCAGAATCTACAAATGCAGGCATGGTGTTGCCGTTTGAGCCCATAACTATATCATTTGAGAAAGTGAAATACTATATTGATACACCCCAGAAACTGAGAGGGCAAGGTTTCCCACCAAATCGACTACAGCTTCTTCAAGACATAACTGGTGCATTTAGACCTGGAATTCTCACAGCTTTGATGGGAGTTAGTGGAGCCGGAAAAACGACACTGATGGATGTTCTTTCTGGAAGAAAAACTGGTGGAATTATTGAAGGAGACATTAGAATTGGAGGGTACCCAAAAGTTCAAGAAACATATGCAAGAATATCTGGGTATTGCGAGCAAAGTGACATACATTCTCCACAGCTTACAGTAGAAGAGTCAGTAGCATACTCAGCTTGGTTACGCTTGTCATCCCAGATTGACAGAAATACTAGAGCTGAGTTTGTGAAAGAAGTACTTCAAATGATAGAGCTTGATGAAATCAAAGACGAGTTAGTTGGCATACCAGGTGGTAGTGGTATATCAACTGAACAGCGTAAAAGGCTAACAATTGCAGTGGAGCTTGTTTCAAATCCATCTGTTATATTCATGGACGAACCCACGTCTGGTTTAGATGCCAGAGCAGCTGCAATAGTCATGCGAGTTGTGAAAAATATTGTCAGTACAAGGAGGACTGTTGTATGCACTATTCATCAGCCAAGTATTGACATATTTGAAGCCTTCGATGAGCTTCTTTTGATGAAAAGAGGAGGACAAGTAATATACTGTGGAGAACTGGGTCAAGATTCGAGTAAGCTTATCCAATACTTTGAGGGTATTTCTGGGGTTCCAAAAATCAAAGATAACTACAACCCAGCAACATGGATGTTAGAAGTCACGAGTCCTTCTGCAGAAGCCAAACTTGCCATAGATTTTGCTCAGCTTTATAGAGAATCAGATTTATGCCAGAAGAACAATGACCTAGTTAGAGAACTCAGCTTTCCAGTAGAAGGCTCAAAGGAATTGAATTTTCCTACTCGCTTCCCGCAAAATGGATGGGAGCAATTTAAAGCATGTCTCTGGAAGAGACATCTCTCCTACTGGAGAAATCCAAAATATAACCTGGGGCGATTGTTAATCACTGCTGCATCCTCTTTGATATTTGGAGCACTTCTTTGGCAGAAAGGACAGAAAATAAATGGTGAACAGGAGTTCTTCAGCATTCTAGGATCAATATTTATTTTACAACAATGCATTGGAATTGGTAACAGTTCCTCAGTTTTACCATTTGTCGCTTCTGAGCGCAATGTTGTATACCGAGAAAGGTTTGCTGGAATGTACTCCTCATGGGCATATTCTTTATCCCAGGTAATCATTGAACTTCCCTATATTTTCCTTCAAGCAGTTCTGTTTTCGACCATTACATATCCAACCATTGGTTTCCACTGGTCACTTTACAAAGTATTCTGGTATGTGTATGCTATGTTTTGTACGCTGCTCTACTTCACTTACTTCGGTATATTGGTTGCTTCTGTGACCCCAACTTTCCAAGTAGCTTCAGTATTATCAAGTTTCTGCTACACAATGTTGAATCTGTTCTCGGGATTCCTAATACCTCAACCAAACATTCCTAAATGGTGGGTTTGGGGCTACTGGATTTCTCCTTTATCCTGGTCCTTGAAGGGTATTCTCACTTCGCAGTATGGGGACCTAAACAAGGAAATAGTACTCCGGGGGGAGGAAAAGACTATCAGTGCCTTCCTAGGAAGCATTTATGGGTATAAATACGATGATCTAGGAATTGTAGGAATTGTTCTTCTTGCATATTCACTAGTTTTCGCATTTGCGTTTGCACTTGCTACAGAAAAACTAAACTTCCAGAGGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1437

Amino Acids

162.83

Weight (kDa)

7.98

Isoelectric Point (pI)

39.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 174 - 355 2.4e-15 ABC transporter
ABC2_membrane PF01061 510 - 721 9.1e-43 ABC-2 type transporter
PDR_assoc PF08370 726 - 788 2.2e-19 Plant PDR ABC transporter associated
ABC_tran PF00005 865 - 1017 5.5e-19 ABC transporter
ABC2_membrane PF01061 1162 - 1376 2.2e-56 ABC-2 type transporter
ABC2_membrane_3 PF12698 1208 - 1422 3.5e-06 ABC-2 family transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000472)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15230 AT4G15230 AT4G15230 AT4G15230 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15236
fragaria_vesca FvH4_4g21360 FvH4_4g21360 FvH4_4g21390 FvH4_6g49431 FvH4_6g49450
malus_domestica MD09G1044300.v1.1 MD16G1137900.v1.1 MD16G1138000.v1.1
prunus_persica Prupe.1G106800_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.3G273300_v2.0.a1
pyrus_communis pycom111g03460 pycom16g12210
rosa_chinensis RchiOBHm_Chr2g0169331 RchiOBHm_Chr2g0169361 RchiOBHm_Chr2g0169371 RchiOBHm_Chr2g0169381 RchiOBHm_Chr2g0169411 RchiOBHm_Chr4g0427141 RchiOBHm_Chr4g0427321
rosa_laevigata RLG00000007252 RLG00000007258 RLG00000013974 RLG00000021856 RLG00000021857 RLG00000021858 RLG00000021859
rosa_multiflora Rmu_sc0000648.1_g000007 Rmu_sc0000648.1_g000009 Rmu_sc0003813.1_g000016 Rmu_sc0009165.1_g000008 Rmu_sc0022554.1_g000001
rosa_roxburghii Rroxscaffold_2G00082200 Rroxscaffold_2G00082230 Rroxscaffold_5G00368960 Rroxscaffold_5G00369010 Rroxscaffold_7G00200390
rosa_rugosa Rorug02G0539500 Rorug02G0539600 Rorug02G0539700 Rorug04G0216400 Rorug04G0216500 Rorug04G0216500 Rorug04G0216600
rosa_samantha Rh2AG610800 Rh2AG611000 Rh2AG611100 Rh2CG592800 Rh2CG593000 Rh2CG593200 Rh2DG634900 Rh2DG635100 Rh2DG635200 Rh2DG635400 Rh4AG272200 Rh4AG272900 Rh4DG275600 Rh4DG276000
rosa_wichuraiana Rw2G050640 Rw2G050660 Rw2G050670 Rw2G050680 Rw4G023610 Rw4G023680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 669
AasI GACNNNNNNGTC 1 cut(s) 1424
Acc65I GGTACC 1 cut(s) 2736
AccB1I GGYRCC 1 cut(s) 2736
AccB7I CCANNNNNTGG 3 cut(s) 584, 1324, 3860
AccI GTMKAC 4 cut(s) 1230, 1662, 2442, 3738
AccIII TCCGGA 1 cut(s) 1174
AciI CCGC 3 cut(s) 534, 1275, 3470
AclWI GGATC 5 cut(s) 925, 1347, 1663, 2026, 3661
AcsI RAATTY 8 cut(s) 385, 516, 987, 1839, 2159, 2400, 2625, 3451
AcuI CTGAAG 9 cut(s) 60, 519, 1148, 1236, 1909, 2227, 2445, 3625, 3966
AhdI GACNNNNNGTC 1 cut(s) 1766
AhlI ACTAGT 3 cut(s) 1621, 2435, 4255
AjiI CACGTC 1 cut(s) 3048
AjnI CCWGG 9 cut(s) 252, 577, 777, 2341, 2620, 2950, 3546, 3789, 4094
Alw21I GWGCWC 2 cut(s) 74, 3595
Alw26I GTCTC 4 cut(s) 1172, 2712, 3505, 3506
AlwI GGATC 5 cut(s) 925, 1347, 1663, 2026, 3661
AlwNI CAGNNNCTG 2 cut(s) 2021, 2554
Ama87I CYCGRG 1 cut(s) 4024
Aor13HI TCCGGA 1 cut(s) 1174
AoxI GGCC 5 cut(s) 876, 1036, 1075, 1238, 2209
ApeKI GCWGC 9 cut(s) 11, 121, 131, 1117, 1826, 3068, 3071, 3569, 3919
ApoI RAATTY 8 cut(s) 385, 516, 987, 1839, 2159, 2400, 2625, 3451
Asp700I GAANNNNTTC 6 cut(s) 162, 414, 1557, 2005, 2901, 4019
Asp718I GGTACC 1 cut(s) 2736
AspA2I CCTAGG 1 cut(s) 4186
AspLEI GCGC 1 cut(s) 3729
AspS9I GGNCC 7 cut(s) 87, 572, 1037, 1238, 1996, 4098, 4132
AsuC2I CCSGG 3 cut(s) 848, 2231, 4157
AsuHPI GGTGA 2 cut(s) 1034, 3638
AsuII TTCGAA 1 cut(s) 166
AvaI CYCGRG 1 cut(s) 4024
AvaII GGWCC 5 cut(s) 87, 572, 1996, 4098, 4132
AvrII CCTAGG 1 cut(s) 4186
BanI GGYRCC 1 cut(s) 2736
BanII GRGCYC 1 cut(s) 2502
BarI GAAGNNNNNNTAC 4 cut(s) 154, 186, 4102, 4134
BauI CACGAG 2 cut(s) 1949, 3322
BbsI GAAGAC 3 cut(s) 41, 701, 1762
Bbv12I GWGCWC 2 cut(s) 74, 3595
BbvI GCAGC 8 cut(s) 108, 118, 1129, 1813, 3058, 3080, 3556, 3906
BccI CCATC 7 cut(s) 1204, 1838, 2287, 2635, 2668, 3028, 3474
BceAI ACGGC 1 cut(s) 2476
BcgI CGANNNNNNTGC 4 cut(s) 1335, 1369, 1655, 1689
BciT130I CCWGG 9 cut(s) 254, 579, 779, 2343, 2622, 2952, 3548, 3791, 4096
BclI TGATCA 2 cut(s) 202, 1465
BcnI CCSGG 3 cut(s) 848, 2231, 4157
BcoDI GTCTC 4 cut(s) 1172, 2712, 3505, 3506
BcuI ACTAGT 3 cut(s) 1621, 2435, 4255
BfmI CTRYAG 9 cut(s) 12, 122, 1115, 1375, 1734, 2205, 2585, 3333, 4284
BisI GCNGC 9 cut(s) 12, 122, 132, 1118, 1827, 3069, 3072, 3570, 3920
BlnI CCTAGG 1 cut(s) 4186
BlpI GCTNAGC 1 cut(s) 3363
BlsI GCNGC 9 cut(s) 13, 123, 133, 1119, 1828, 3070, 3073, 3571, 3921
BmcAI AGTACT 4 cut(s) 1352, 1682, 2901, 4152
Bme18I GGWCC 5 cut(s) 87, 572, 1996, 4098, 4132
BmeRI GACNNNNNGTC 1 cut(s) 1766
BmeT110I CYCGRG 1 cut(s) 4024
BmgBI CACGTC 1 cut(s) 3048
BmgT120I GGNCC 7 cut(s) 87, 572, 1037, 1238, 1996, 4098, 4132
BmiI GGNNCC 6 cut(s) 852, 2340, 2656, 2738, 3273, 4133
BmrI ACTGGG 1 cut(s) 3230
BmsI GCATC 7 cut(s) 65, 479, 1849, 1963, 1997, 3049, 3581
BmuI ACTGGG 1 cut(s) 3230
BpiI GAAGAC 3 cut(s) 41, 701, 1762
BpmI CTGGAG 3 cut(s) 561, 2325, 3547
Bpu1102I GCTNAGC 1 cut(s) 3363
Bpu14I TTCGAA 1 cut(s) 166
BpuEI CTTGAG 2 cut(s) 269, 620
BpuMI CCSGG 3 cut(s) 848, 2231, 4157
BsaAI YACGTR 1 cut(s) 1646
BsaWI WCCGGW 1 cut(s) 1174
BsaXI ACNNNNNCTCC 2 cut(s) 3186, 3216
Bse1I ACTGG 8 cut(s) 762, 2611, 2704, 3225, 3428, 3530, 3875, 4082
Bse3DI GCAATG 5 cut(s) 981, 1614, 1732, 1850, 3736
BseAI TCCGGA 1 cut(s) 1174
BseBI CCWGG 9 cut(s) 254, 579, 779, 2343, 2622, 2952, 3548, 3791, 4096
BseMI GCAATG 5 cut(s) 981, 1614, 1732, 1850, 3736
BseNI ACTGG 8 cut(s) 762, 2611, 2704, 3225, 3428, 3530, 3875, 4082
BseRI GAGGAG 3 cut(s) 1460, 3207, 3757
BseXI GCAGC 8 cut(s) 108, 118, 1129, 1813, 3058, 3080, 3556, 3906
BseYI CCCAGC 3 cut(s) 1039, 1801, 3298
BshFI GGCC 5 cut(s) 878, 1038, 1077, 1240, 2211
BshNI GGYRCC 1 cut(s) 2736
BsiHKAI GWGCWC 2 cut(s) 74, 3595
BsiHKCI CYCGRG 1 cut(s) 4024
BsiSI CCGG 6 cut(s) 848, 1175, 1823, 2230, 2658, 4156
BslFI GGGAC 5 cut(s) 363, 830, 983, 1259, 4145
BsmAI GTCTC 4 cut(s) 1172, 2712, 3505, 3506
BsmFI GGGAC 5 cut(s) 363, 830, 983, 1259, 4145
BsmI GAATGC 1 cut(s) 3645
BsnI GGCC 5 cut(s) 878, 1038, 1077, 1240, 2211
BsoBI CYCGRG 1 cut(s) 4024
Bsp119I TTCGAA 1 cut(s) 166
Bsp1286I GDGCHC 3 cut(s) 74, 2502, 3595
Bsp13I TCCGGA 1 cut(s) 1174
Bsp143I GATC 8 cut(s) 202, 930, 1339, 1465, 1668, 2018, 3653, 4216
Bsp1720I GCTNAGC 1 cut(s) 3363
Bsp19I CCATGG 1 cut(s) 1234
BspACI CCGC 3 cut(s) 534, 1275, 3470
BspANI GGCC 5 cut(s) 878, 1038, 1077, 1240, 2211
BspEI TCCGGA 1 cut(s) 1174
BspHI TCATGA 4 cut(s) 205, 471, 1563, 2368
BspLI GGNNCC 6 cut(s) 852, 2340, 2656, 2738, 3273, 4133
BspMAI CTGCAG 5 cut(s) 16, 126, 1119, 1738, 3337
BspPI GGATC 5 cut(s) 925, 1347, 1663, 2026, 3661
BspT104I TTCGAA 1 cut(s) 166
BspT107I GGYRCC 1 cut(s) 2736
BsrDI GCAATG 5 cut(s) 981, 1614, 1732, 1850, 3736
BsrI ACTGG 8 cut(s) 762, 2611, 2704, 3225, 3428, 3530, 3875, 4082
BssMI GATC 8 cut(s) 202, 930, 1339, 1465, 1668, 2018, 3653, 4216
BssNAI GTATAC 2 cut(s) 1231, 3739
BssSI CACGAG 2 cut(s) 1949, 3322
BssT1I CCWWGG 8 cut(s) 934, 1234, 1241, 1992, 2093, 2171, 2305, 4186
Bst1107I GTATAC 2 cut(s) 1231, 3739
Bst2BI CACGAG 2 cut(s) 1949, 3322
Bst2UI CCWGG 9 cut(s) 254, 579, 779, 2343, 2622, 2952, 3548, 3791, 4096
Bst4CI ACNGT 8 cut(s) 367, 745, 1379, 1904, 2815, 3121, 3212, 3695
Bst6I CTCTTC 4 cut(s) 1437, 1562, 2814, 3503
BstBAI YACGTR 1 cut(s) 1646
BstBI TTCGAA 1 cut(s) 166
BstC8I GCNNGC 8 cut(s) 54, 834, 1457, 1540, 2213, 2217, 2479, 2783
BstDSI CCRYGG 1 cut(s) 1234
BstENI CCTNNNNNAGG 2 cut(s) 1319, 4139
BstHHI GCGC 1 cut(s) 3729
BstKTI GATC 8 cut(s) 205, 933, 1342, 1468, 1671, 2021, 3656, 4219
BstMAI GTCTC 4 cut(s) 1172, 2712, 3505, 3506
BstMBI GATC 8 cut(s) 202, 930, 1339, 1465, 1668, 2018, 3653, 4216
BstMWI GCNNNNNNNGC 9 cut(s) 789, 971, 1083, 1456, 2497, 2837, 2986, 3068, 3347
BstNI CCWGG 9 cut(s) 254, 579, 779, 2343, 2622, 2952, 3548, 3791, 4096
BstNSI RCATGY 5 cut(s) 56, 1490, 1542, 2319, 3501
BstSFI CTRYAG 9 cut(s) 12, 122, 1115, 1375, 1734, 2205, 2585, 3333, 4284
BstSNI TACGTA 1 cut(s) 1646
BstV1I GCAGC 8 cut(s) 108, 118, 1129, 1813, 3058, 3080, 3556, 3906
BstV2I GAAGAC 3 cut(s) 41, 701, 1762
BstX2I RGATCY 3 cut(s) 930, 1668, 2018
BstXI CCANNNNNNTGG 4 cut(s) 441, 785, 1150, 3306
BstYI RGATCY 3 cut(s) 930, 1668, 2018
BstZ17I GTATAC 2 cut(s) 1231, 3739
BsuRI GGCC 5 cut(s) 878, 1038, 1077, 1240, 2211
BtgI CCRYGG 1 cut(s) 1234
BtrI CACGTC 1 cut(s) 3048
BtsI GCAGTG 3 cut(s) 131, 3006, 3564
Cac8I GCNNGC 8 cut(s) 54, 834, 1457, 1540, 2213, 2217, 2479, 2783
CaiI CAGNNNCTG 2 cut(s) 2021, 2554
CciI TCATGA 4 cut(s) 205, 471, 1563, 2368
CfoI GCGC 1 cut(s) 3729
Cfr13I GGNCC 7 cut(s) 87, 572, 1037, 1238, 1996, 4098, 4132
CseI GACGC 1 cut(s) 980
CsiI ACCWGGT 1 cut(s) 2950
CspCI CAANNNNNGTGG 2 cut(s) 2982, 3017
DpnI GATC 8 cut(s) 204, 932, 1341, 1467, 1670, 2020, 3655, 4218
DpnII GATC 8 cut(s) 202, 930, 1339, 1465, 1668, 2018, 3653, 4216
DraI TTTAAA 1 cut(s) 3493
DrdI GACNNNNNNGTC 1 cut(s) 1424
DriI GACNNNNNGTC 1 cut(s) 1766
DseDI GACNNNNNNGTC 1 cut(s) 1424
Eam1104I CTCTTC 4 cut(s) 1437, 1562, 2814, 3503
Eam1105I GACNNNNNGTC 1 cut(s) 1766
EarI CTCTTC 4 cut(s) 1437, 1562, 2814, 3503
Eco105I TACGTA 1 cut(s) 1646
Eco130I CCWWGG 8 cut(s) 934, 1234, 1241, 1992, 2093, 2171, 2305, 4186
Eco147I AGGCCT 1 cut(s) 1077
Eco24I GRGCYC 1 cut(s) 2502
Eco32I GATATC 4 cut(s) 1068, 1743, 1789, 2265
Eco47I GGWCC 5 cut(s) 87, 572, 1996, 4098, 4132
Eco57I CTGAAG 9 cut(s) 60, 519, 1148, 1236, 1909, 2227, 2445, 3625, 3966
Eco88I CYCGRG 1 cut(s) 4024
EcoNI CCTNNNNNAGG 2 cut(s) 1319, 4139
EcoO109I RGGNCCY 1 cut(s) 4132
EcoRI GAATTC 1 cut(s) 2625
EcoRII CCWGG 9 cut(s) 252, 577, 777, 2341, 2620, 2950, 3546, 3789, 4094
EcoRV GATATC 4 cut(s) 1068, 1743, 1789, 2265
EcoT14I CCWWGG 8 cut(s) 934, 1234, 1241, 1992, 2093, 2171, 2305, 4186
EcoT22I ATGCAT 3 cut(s) 58, 1864, 3680
EcoT38I GRGCYC 1 cut(s) 2502
ErhI CCWWGG 8 cut(s) 934, 1234, 1241, 1992, 2093, 2171, 2305, 4186
FalI AAGNNNNNCTT 8 cut(s) 1434, 1466, 1527, 1559, 2886, 2918, 3330, 3362
FaqI GGGAC 5 cut(s) 363, 830, 983, 1259, 4145
FauI CCCGC 1 cut(s) 3477
FauNDI CATATG 1 cut(s) 2758
FbaI TGATCA 2 cut(s) 202, 1465
FblI GTMKAC 4 cut(s) 1230, 1662, 2442, 3738
Fnu4HI GCNGC 9 cut(s) 12, 122, 132, 1118, 1827, 3069, 3072, 3570, 3920
FriOI GRGCYC 1 cut(s) 2502
Fsp4HI GCNGC 9 cut(s) 12, 122, 132, 1118, 1827, 3069, 3072, 3570, 3920
GlaI GCGC 1 cut(s) 3728
GluI GCNGC 9 cut(s) 12, 122, 132, 1118, 1827, 3069, 3072, 3570, 3920
GsaI CCCAGC 3 cut(s) 1043, 1805, 3302
GsuI CTGGAG 3 cut(s) 561, 2325, 3547
HaeIII GGCC 5 cut(s) 878, 1038, 1077, 1240, 2211
HapII CCGG 6 cut(s) 848, 1175, 1823, 2230, 2658, 4156
HgaI GACGC 1 cut(s) 980
HhaI GCGC 1 cut(s) 3729
Hin6I GCGC 1 cut(s) 3727
HinP1I GCGC 1 cut(s) 3727
HincII GTYRAC 1 cut(s) 1663
HindII GTYRAC 1 cut(s) 1663
HindIII AAGCTT 3 cut(s) 1436, 2217, 3239
HpaII CCGG 6 cut(s) 848, 1175, 1823, 2230, 2658, 4156
HphI GGTGA 2 cut(s) 1034, 3638
Hpy166II GTNNAC 6 cut(s) 1231, 1663, 2313, 2443, 3629, 3739
Hpy8I GTNNAC 6 cut(s) 1231, 1663, 2313, 2443, 3629, 3739
Hpy99I CGWCG 1 cut(s) 1667
HpyCH4III ACNGT 8 cut(s) 367, 745, 1379, 1904, 2815, 3121, 3212, 3695
HpyCH4IV ACGT 4 cut(s) 160, 259, 1645, 3047
HpyF10VI GCNNNNNNNGC 9 cut(s) 789, 971, 1083, 1456, 2497, 2837, 2986, 3068, 3347
HpySE526I ACGT 4 cut(s) 160, 259, 1645, 3047
HspAI GCGC 1 cut(s) 3727
Kpn2I TCCGGA 1 cut(s) 1174
KpnI GGTACC 1 cut(s) 2740
Ksp22I TGATCA 2 cut(s) 202, 1465
Kzo9I GATC 8 cut(s) 202, 930, 1339, 1465, 1668, 2018, 3653, 4216
Lsp1109I GCAGC 8 cut(s) 108, 118, 1129, 1813, 3058, 3080, 3556, 3906
LweI GCATC 7 cut(s) 65, 479, 1849, 1963, 1997, 3049, 3581
MabI ACCWGGT 1 cut(s) 2950
MaeII ACGT 4 cut(s) 160, 259, 1645, 3047
MalI GATC 8 cut(s) 204, 932, 1341, 1467, 1670, 2020, 3655, 4218
MboI GATC 8 cut(s) 202, 930, 1339, 1465, 1668, 2018, 3653, 4216
MfeI CAATTG 4 cut(s) 356, 885, 1121, 2994
MflI RGATCY 3 cut(s) 930, 1668, 2018
MhlI GDGCHC 3 cut(s) 74, 2502, 3595
MlyI GAGTC 4 cut(s) 553, 1120, 2831, 3334
MmeI TCCRAC 4 cut(s) 458, 1408, 2079, 3878
Mph1103I ATGCAT 3 cut(s) 58, 1864, 3680
MroI TCCGGA 1 cut(s) 1174
MroXI GAANNNNTTC 6 cut(s) 162, 414, 1557, 2005, 2901, 4019
MseI TTAA 6 cut(s) 1413, 2039, 2222, 2226, 3492, 3560
MslI CAYNNNNRTG 3 cut(s) 51, 869, 1485
MspA1I CMGCKG 2 cut(s) 534, 3071
MspI CCGG 6 cut(s) 848, 1175, 1823, 2230, 2658, 4156
MunI CAATTG 4 cut(s) 356, 885, 1121, 2994
Mva1269I GAATGC 1 cut(s) 3645
MvaI CCWGG 9 cut(s) 254, 579, 779, 2343, 2622, 2952, 3548, 3791, 4096
MwoI GCNNNNNNNGC 9 cut(s) 789, 971, 1083, 1456, 2497, 2837, 2986, 3068, 3347
NciI CCSGG 3 cut(s) 848, 2231, 4157
NcoI CCATGG 1 cut(s) 1234
NdeI CATATG 1 cut(s) 2758
NdeII GATC 8 cut(s) 202, 930, 1339, 1465, 1668, 2018, 3653, 4216
NlaIV GGNNCC 6 cut(s) 852, 2340, 2656, 2738, 3273, 4133
NmuCI GTSAC 4 cut(s) 2789, 3319, 3873, 3958
NsiI ATGCAT 3 cut(s) 58, 1864, 3680
NspI RCATGY 5 cut(s) 56, 1490, 1542, 2319, 3501
NspV TTCGAA 1 cut(s) 166
PacI TTAATTAA 1 cut(s) 2226
PaeI GCATGC 2 cut(s) 56, 1542
PagI TCATGA 4 cut(s) 205, 471, 1563, 2368
PceI AGGCCT 1 cut(s) 1077
PctI GAATGC 1 cut(s) 3645
PdmI GAANNNNTTC 6 cut(s) 162, 414, 1557, 2005, 2901, 4019
PfeI GAWTC 7 cut(s) 1139, 2376, 2465, 3230, 3377, 4015, 4029
PflMI CCANNNNNTGG 3 cut(s) 584, 1324, 3860
PfoI TCCNGGA 1 cut(s) 846
PkrI GCNGC 9 cut(s) 13, 123, 133, 1119, 1828, 3070, 3073, 3571, 3921
PleI GAGTC 4 cut(s) 553, 1120, 2830, 3333
PpsI GAGTC 4 cut(s) 553, 1120, 2830, 3333
Ppu21I YACGTR 1 cut(s) 1646
PpuMI RGGWCCY 1 cut(s) 4132
PsiI TTATAA 1 cut(s) 669
Psp5II RGGWCCY 1 cut(s) 4132
Psp6I CCWGG 9 cut(s) 252, 577, 777, 2341, 2620, 2950, 3546, 3789, 4094
PspFI CCCAGC 3 cut(s) 1039, 1801, 3298
PspGI CCWGG 9 cut(s) 252, 577, 777, 2341, 2620, 2950, 3546, 3789, 4094
PspN4I GGNNCC 6 cut(s) 852, 2340, 2656, 2738, 3273, 4133
PspPI GGNCC 7 cut(s) 87, 572, 1037, 1238, 1996, 4098, 4132
PspPPI RGGWCCY 1 cut(s) 4132
PsrI GAACNNNNNNTAC 2 cut(s) 4221, 4253
PstI CTGCAG 5 cut(s) 16, 126, 1119, 1738, 3337
PstNI CAGNNNCTG 2 cut(s) 2021, 2554
PsuI RGATCY 3 cut(s) 930, 1668, 2018
PvuII CAGCTG 1 cut(s) 3071
RseI CAYNNNNRTG 3 cut(s) 51, 869, 1485
SalI GTCGAC 1 cut(s) 1661
SaqAI TTAA 6 cut(s) 1413, 2039, 2222, 2226, 3492, 3560
SatI GCNGC 9 cut(s) 12, 122, 132, 1118, 1827, 3069, 3072, 3570, 3920
Sau3AI GATC 8 cut(s) 202, 930, 1339, 1465, 1668, 2018, 3653, 4216
Sau96I GGNCC 7 cut(s) 87, 572, 1037, 1238, 1996, 4098, 4132
ScaI AGTACT 4 cut(s) 1352, 1682, 2901, 4152
SchI GAGTC 4 cut(s) 553, 1120, 2831, 3334
SduI GDGCHC 3 cut(s) 74, 2502, 3595
SexAI ACCWGGT 1 cut(s) 2950
SfaNI GCATC 7 cut(s) 65, 479, 1849, 1963, 1997, 3049, 3581
SfcI CTRYAG 9 cut(s) 12, 122, 1115, 1375, 1734, 2205, 2585, 3333, 4284
SfuI TTCGAA 1 cut(s) 166
SinI GGWCC 5 cut(s) 87, 572, 1996, 4098, 4132
SmiMI CAYNNNNRTG 3 cut(s) 51, 869, 1485
SmlI CTYRAG 2 cut(s) 284, 635
SmoI CTYRAG 2 cut(s) 284, 635
SnaBI TACGTA 1 cut(s) 1646
SpeI ACTAGT 3 cut(s) 1621, 2435, 4255
SphI GCATGC 2 cut(s) 56, 1542
SseBI AGGCCT 1 cut(s) 1077
SsiI CCGC 3 cut(s) 534, 1275, 3470
SspI AATATT 2 cut(s) 3100, 3660
StuI AGGCCT 1 cut(s) 1077
StyI CCWWGG 8 cut(s) 934, 1234, 1241, 1992, 2093, 2171, 2305, 4186
TaaI ACNGT 8 cut(s) 367, 745, 1379, 1904, 2815, 3121, 3212, 3695
TaiI ACGT 4 cut(s) 163, 262, 1648, 3050
TaqI TCGA 7 cut(s) 166, 820, 1662, 2581, 3168, 3233, 3839
TatI WGTACW 6 cut(s) 1350, 1680, 2899, 3107, 3762, 4150
TfiI GAWTC 7 cut(s) 1139, 2376, 2465, 3230, 3377, 4015, 4029
Tru1I TTAA 6 cut(s) 1413, 2039, 2222, 2226, 3492, 3560
Tru9I TTAA 6 cut(s) 1413, 2039, 2222, 2226, 3492, 3560
TseFI GTSAC 4 cut(s) 2789, 3319, 3873, 3958
TseI GCWGC 9 cut(s) 11, 121, 131, 1117, 1826, 3068, 3071, 3569, 3919
Tsp45I GTSAC 4 cut(s) 2789, 3319, 3873, 3958
TspGWI ACGGA 1 cut(s) 2112
Van91I CCANNNNNTGG 3 cut(s) 584, 1324, 3860
VpaK11BI GGWCC 5 cut(s) 87, 572, 1996, 4098, 4132
XagI CCTNNNNNAGG 2 cut(s) 1319, 4139
XapI RAATTY 8 cut(s) 385, 516, 987, 1839, 2159, 2400, 2625, 3451
XbaI TCTAGA 2 cut(s) 1547, 1757
XceI RCATGY 5 cut(s) 56, 1490, 1542, 2319, 3501
XmaJI CCTAGG 1 cut(s) 4186
XmiI GTMKAC 4 cut(s) 1230, 1662, 2442, 3738
XmnI GAANNNNTTC 6 cut(s) 162, 414, 1557, 2005, 2901, 4019
ZrmI AGTACT 4 cut(s) 1352, 1682, 2901, 4152
Zsp2I ATGCAT 3 cut(s) 58, 1864, 3680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.