Rh4DG275600
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000472
Physical Location & Seq
Forward (+)
49486519 .. 49493863
7345 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG275600.1

Sequence Viewer

Length: 4299 bp
ATGGAGCTAACACAAGTTAAGAGAGATGTCCACTCATCGTTTCGACACCAAGCTTCGAGCTACTCAATCAAAGCGGAGGATGAGGAAACTCAGCTGCAGTGGGCTGCCATTGAGAGATTGCCTACATTCAGAAGGCTGAGGACCTCACTTTTCGACCTCGGTGATAGCGAAAATGGAAAAACCAGTGGCAAAGAGTGTGCAGGGAAGAAGGTGGTTGATGTTACCAAGTTGGGAGCAGATGAGAGGCATTTTTTCATTGAGAAGATCATAAAGCACATTGAGCATGATAATCTGAGGCTGCTACAAAAAATGAGAGAAAGGATAGACAGAGTGAATGTGAAATTGCCAACTGTGGAGGTGAGGTACAAGAGTTTGTGTGTGGAAGCCGAGTGTGAAGTAGTACAAGGAAAGCCGCTCCCGACGCTGTGGAACTCCTTTCTAAGCTTGTTATCAGTTTTTCGAAAGGTAATGTGGTGCAAGTCTCAAGATGCAAAGATTAGCGTTCTCACAGACGTCAGTGGCGTCATCAAACCTTCAAGGCTTACTCTCCTTCTTGGTCCCCCGGGGTGTGGTAAAACGACCTTCTTATTGGCACTTGCCGGAAAACTGGATAAATCCCTGAAGGTTGCAGGGGAGATTTCATACAATGGTTACAAGCTAGATGAGTTTGTTCCTCAAAAAACTTCAGCGTACATAAGCCAACACGACCTTCACGTACCTGAGATGACTGTAAGAGAAACAATCGAGTTTTCAGCCCGCTGTCAAGGTGTTGGAAGCAGAGCTGATATAATGCTAGAGGTTAGCAGGAGAGAAAAGGAAGCAGGAATTGTTCCTGATCCAGATATTGACACCTACATGAAGGCAATCTCAGTTGAAGGACAAAAGAAAAACCTCCAAACTGACTATGTTTTGAAGATGCTAGGACTAGATATCTGCAGTGACACAATGGTTGGTGATGCATTACAAAGAGGTATTTCGGGTGGCCAAAAGAAAAGGCTGACAACAGGAGAGATGATTGTCGGTCCCACAAAAGCGCTATTTATGGATGAAATATCAACTGGATTAGACAGCTCTACGACTTTTCAGATAGTCACATATCTGCAGCAGTTGGTGCACATAACAGATACAACTGCATTGGTGTCACTTCTACAACCAGCACCTGAGACATTCGATTTATTTGATGATGTGATACTAATGGCAGAGGGGAAGATAGTTTACCATGGTCCTCGGAGTCATGCACTTCAGTTTTTCGAAGATTGTGGTTTTAAGTGCCCTCCAAGAAAGGGTGCTGCAGATTATCTTCAGGAGTTAATCTCAAAGAAGGATCAAGCACAATACTGGAAGCATGATAACATTCCCTATGATTATGTTTCTGTGGACAAATTCTCTCAGCTTTTCAGAGCAAGTTACTTGGGAAAGAAGTTAATTGATGAGCTCTCAAAACCATATGAGAAATCACCGGGCCAGGATAAGGCTTTATCATTTAGCATCTACTCTGTCAGAAAATGGGAATTGTTCAAAGCTTGTATGGGTAGAGAGCTGCTTCTTATGAAACGAAATTCCTTTGTTTATGTATTCAAAACAGCGCAGCTTGTCATCACTGCACTTTTGACAACGACAGTATTCATACGTACTCAGATGGATGTGGATTTGACAAGTGCAAATTATTTTATGGGTGCGTTGTTCTATACACTTGTGAGACTTATGACCAATGGAGTTGCAGAACTGTCCTTGACTATCACTAGACTACCAGTGGTTGATAAGCAAAGAGCCTTCTATCTTTATCCAGCATGGTCCTATTCAATTCCAGCCTCTCTCCTGAAGATTCCATTTTCACTGAGTGATGCGGTGTTGTGGACAGCAATAACTTACTATGGTGTTGGGTATAGCCCTGAAATAAAAAGGTTCTTCTGCCAGCTCCTTGTGCTGTTTGCTCTGCATCTTGCATCAACATCCATGTGTCGTTTGATTGCAGTAATTTTCCGATCCATGGTTGTTGCAACAACTATTGGTACTTTAATCTTGGTACTAATGTTTGTATTTGGAGGCTTCATTTTGCCACCACCCTCATTACCTGTTTGGTTGAAGTGGGGATTCTGGTGTTCTCCTATGACTTATGGGGAAATAGCTGCAAGTCTGAATGAATTCCGTGCTCCTCGATGGCAAAAGGTTTCGAGAGGAAATACAACCTTAGGGAATGAAATTCTAACCAGTCATGGTTTGAACTTTGGTAGCTACTTCTACTGGATATCAGTAGGGGCTTTATTCGGGTTCACAGCTATTTTCGATCTTGGATTTGTTTTAGCATTAACTTACTTAAAATCTCCAAAGATGTCTCGTGCTATTATTTCGAAAAAAAGATTATCTCAACTCAAAGGACAAGATTCTTGGAACAAGACCGGTGCAAAGTCAGATAATGTATCAACCCCAGCTGATTTTTCCCAAACCATGGAAGAAACAGGGAAAATGGTTCTGCCATTTGAGCCGCTGACAATCTCATTTAAGGATGTGCAGTACAGTGTTGACATCCCACCGGAAATGAAAAGGCACAGTTTCGATCAGAAAAAACTTCAGCTGCTTAAAGATATTACAGGAGCATTTAGGCCGGGAGTTCTTACAGCATTGATGGGAGTCAGTGGGGCTGGGAAAACAACTCTCATGGATGTTCTTTCGGGAAGGAAAACTGGAGGCACTATTGAAGGAGATATAAGAATAGGAGGCTATCCCAAAGTCCAGAAGACATTTGCTAGAATATCAGGTTACTGTGAACAATTTGATATACATTCTCCATGCATCACAGTAGGAGAGTCGGTTATGTTTTCAGCTTGGTTGAGGTTGCCACCCGAAATTGATCAAGACACAAAAGCTAAATTTGTAGAAGAAGTTATTGAAACAATTGAATTGGAGGATATAAGAGATTCTTTAGTTGGCATTCCTGGCCAAAGTGGCCTATCAACCGAACAGCGCAAAAGGCTAACAATTGCAGTTGAGCTTGTTTCCAATCCATCAATAATATTTATGGATGAACCTACATCAGGACTAGATGCCAGAGCAGCAGCAATTGTCATGCGTGCTGTGAAGAATGTAGTTGCCACAGGAAGAACAACTATTTGCACAATCCACCAACCTAGCATTGATATCTTTGAGGCTTTTGATGAGTTAATTCTGATGAAAATGGGAGGGCAAATTATCTATTCTGGAAGTCTGGGCCATCATTCGAGTAATCTCATTGAATATTTCGAGGGTATTCCTGGTGTACCAAAGATCAAAGATAACTATAACCCCGCAACATGGATGTTAGAAGTTACTTCCTCTTCAGTAGAAGCAGAACTCGGTTTAGATTTTTCGAACATTTATAAAGAATCTACTCTGTATAAAGACACAGTTGAATTGGTGAGACAGATAAGTAAACCAAAGCCAGGCTCAAGAGCTTTGCAATTTTCCACCCAATTTCCACAAAATAGCTGGGTGCAGTTTAGGGCCTGCTTATGGAAACAACACTTATCCTACTGGAGAAGTCCTGAATACAATCTAGCACGTTTCATGTTCATGACTGTTGCCTCAGTGTTGTTTGGTGCAATCTTCTGGCAAAAGGGGAAGGAAATAAATAATGAGCAGGATTTGCTGACTATACTTGGGTCCATGTACATTGCTGTAATATTCCTGGGAATAAACAATTGTTCAACAGTTCTTCCTTATGTGGCAACTGAGCGAACCGTTTTGTACCGCGAAAGATTTGCTGGGATGTACTCATCAAAGGCTTATTCCTTTGCACAGGTGGCCATGGAAATACCTTATACAATGTTGCAAGCAATTTTATATGTGGTCATTACATATCCTACGATAGGCTACTATTGGACTGCTTCCAAGGTTTTTTGGTACTTCTATGCAACCTTCTGTACATTTCTATACTTTGTATACCTTGGGATGTTGTTAGTTTCTTTGAGCTCAGACATGGAAGTAGCTTCGATATTGGCCACTGCAATCTACACCATATTGAATCTTTTCTCCGGCTTCCTCATGCCTGGACCGAAAATTCCGAAGTGGTGGATTTGGTGCTACTGGATCTGTCCTACATCATGGTCACTAAATGGCCTCCTGACTTCACAATATGGAGACATGGACAAAGAGATATTAATTTTTGGAGAGCATAAAACAGTTGCTTCCTTCGTACAAGATTATTATGGTTTTCATCATGATCTTTTACCCCTTGTGGCTATTGCTCTGATTACCTTCCCAATTGCTTTTGCTTTTCTCTTTGCCTATTTTATAGGGAGATTAAATTTCCAAAGGCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1432

Amino Acids

161.57

Weight (kDa)

8.53

Isoelectric Point (pI)

36.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 169 - 350 3e-16 ABC transporter
ABC2_membrane PF01061 505 - 716 3.4e-45 ABC-2 type transporter
PDR_assoc PF08370 721 - 783 6.4e-23 Plant PDR ABC transporter associated
ABC_tran PF00005 860 - 1012 1.7e-19 ABC transporter
ABC2_membrane PF01061 1158 - 1371 3.4e-58 ABC-2 type transporter
ABC2_membrane_3 PF12698 1208 - 1416 7.9e-07 ABC-2 family transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000472)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15230 AT4G15230 AT4G15230 AT4G15230 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15236
fragaria_vesca FvH4_4g21360 FvH4_4g21360 FvH4_4g21390 FvH4_6g49431 FvH4_6g49450
malus_domestica MD09G1044300.v1.1 MD16G1137900.v1.1 MD16G1138000.v1.1
prunus_persica Prupe.1G106800_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.3G273300_v2.0.a1
pyrus_communis pycom111g03460 pycom16g12210
rosa_chinensis RchiOBHm_Chr2g0169331 RchiOBHm_Chr2g0169361 RchiOBHm_Chr2g0169371 RchiOBHm_Chr2g0169381 RchiOBHm_Chr2g0169411 RchiOBHm_Chr4g0427141 RchiOBHm_Chr4g0427321
rosa_laevigata RLG00000007252 RLG00000007258 RLG00000013974 RLG00000021856 RLG00000021857 RLG00000021858 RLG00000021859
rosa_multiflora Rmu_sc0000648.1_g000007 Rmu_sc0000648.1_g000009 Rmu_sc0003813.1_g000016 Rmu_sc0009165.1_g000008 Rmu_sc0022554.1_g000001
rosa_roxburghii Rroxscaffold_2G00082200 Rroxscaffold_2G00082230 Rroxscaffold_5G00368960 Rroxscaffold_5G00369010 Rroxscaffold_7G00200390
rosa_rugosa Rorug02G0539500 Rorug02G0539600 Rorug02G0539700 Rorug04G0216400 Rorug04G0216500 Rorug04G0216500 Rorug04G0216600
rosa_samantha Rh2AG610800 Rh2AG611000 Rh2AG611100 Rh2CG592800 Rh2CG593000 Rh2CG593200 Rh2DG634900 Rh2DG635100 Rh2DG635200 Rh2DG635400 Rh4AG272200 Rh4AG272900 Rh4DG275600 Rh4DG276000
rosa_wichuraiana Rw2G050640 Rw2G050660 Rw2G050670 Rw2G050680 Rw4G023610 Rw4G023680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3357
AatII GACGTC 1 cut(s) 516
AccB7I CCANNNNNTGG 1 cut(s) 2449
AccBSI CCGCTC 1 cut(s) 415
AccI GTMKAC 1 cut(s) 3918
AccII CGCG 1 cut(s) 3729
AciI CCGC 7 cut(s) 74, 413, 757, 1847, 2486, 3285, 3727
AclWI GGATC 4 cut(s) 830, 1332, 1980, 4073
AcoI YGGCCR 4 cut(s) 982, 2938, 3780, 3975
AcsI RAATTY 7 cut(s) 1382, 1558, 2144, 2202, 2870, 4035, 4282
AcuI CTGAAG 7 cut(s) 641, 669, 1226, 1286, 1841, 2555, 3300
AcyI GRCGYC 2 cut(s) 513, 522
AdeI CACNNNGTG 1 cut(s) 1841
AfeI AGCGCT 1 cut(s) 1035
AgeI ACCGGT 1 cut(s) 2399
AjnI CCWGG 6 cut(s) 1464, 2935, 3250, 3418, 3663, 4024
AjuI GAANNNNNNNTTGG 2 cut(s) 693, 725
Alw21I GWGCWC 4 cut(s) 1116, 1437, 2155, 3950
Alw26I GTCTC 6 cut(s) 486, 1157, 1693, 2340, 3391, 4110
Alw44I GTGCAC 1 cut(s) 1112
AlwI GGATC 4 cut(s) 830, 1332, 1980, 4073
AlwNI CAGNNNCTG 1 cut(s) 1160
Ama87I CYCGRG 1 cut(s) 562
Aor51HI AGCGCT 1 cut(s) 1035
ApaLI GTGCAC 1 cut(s) 1112
ApoI RAATTY 7 cut(s) 1382, 1558, 2144, 2202, 2870, 4035, 4282
AseI ATTAAT 1 cut(s) 4136
AsiGI ACCGGT 1 cut(s) 2399
Asp700I GAANNNNTTC 2 cut(s) 2144, 4004
AspLEI GCGC 3 cut(s) 1036, 1588, 2967
AsuC2I CCSGG 4 cut(s) 563, 564, 1461, 2607
AsuHPI GGTGA 5 cut(s) 173, 370, 965, 1449, 3406
AsuII TTCGAA 4 cut(s) 460, 1251, 2351, 3347
AvaI CYCGRG 1 cut(s) 562
AvaII GGWCC 7 cut(s) 141, 557, 1022, 1223, 1794, 3639, 4028
AxyI CCTNAGG 1 cut(s) 2191
BaeGI GKGCMC 2 cut(s) 1116, 1274
BaeI ACNNNNGTAYC 4 cut(s) 355, 388, 2018, 2051
BalI TGGCCA 4 cut(s) 984, 2940, 3782, 3977
BanII GRGCYC 2 cut(s) 1437, 3950
BarI GAAGNNNNNNTAC 2 cut(s) 1199, 1231
BauI CACGAG 1 cut(s) 2337
BbsI GAAGAC 1 cut(s) 2744
Bbv12I GWGCWC 4 cut(s) 1116, 1437, 2155, 3950
BbvCI CCTCAGC 1 cut(s) 137
BccI CCATC 5 cut(s) 1633, 2154, 2620, 3013, 3219
BcgI CGANNNNNNTGC 2 cut(s) 3719, 3753
BciT130I CCWGG 6 cut(s) 1466, 2937, 3252, 3420, 3665, 4026
BclI TGATCA 1 cut(s) 2851
BcnI CCSGG 4 cut(s) 563, 564, 1461, 2607
BcoDI GTCTC 6 cut(s) 486, 1157, 1693, 2340, 3391, 4110
BfaI CTAG 9 cut(s) 659, 794, 920, 926, 1743, 2748, 3041, 3129, 3533
BfmI CTRYAG 4 cut(s) 95, 934, 1100, 1290
BfoI RGCGCY 1 cut(s) 1037
BglI GCCNNNNNGGC 1 cut(s) 2946
Bme18I GGWCC 7 cut(s) 141, 557, 1022, 1223, 1794, 3639, 4028
BmeT110I CYCGRG 1 cut(s) 562
BmiI GGNNCC 3 cut(s) 559, 1024, 3640
BmsI GCATC 9 cut(s) 478, 906, 946, 1497, 1834, 1948, 1955, 2802, 3034
BpiI GAAGAC 1 cut(s) 2744
BplI GAGNNNNNCTC 2 cut(s) 1298, 1330
BpmI CTGGAG 2 cut(s) 2706, 3532
Bpu10I CCTNAGC 1 cut(s) 137
Bpu14I TTCGAA 4 cut(s) 460, 1251, 2351, 3347
BpuEI CTTGAG 2 cut(s) 468, 3409
BpuMI CCSGG 4 cut(s) 563, 564, 1461, 2607
BsaAI YACGTR 2 cut(s) 715, 1631
BsaHI GRCGYC 2 cut(s) 513, 522
BsaWI WCCGGW 2 cut(s) 2399, 2533
BsaXI ACNNNNNCTCC 4 cut(s) 347, 377, 2796, 2826
Bse118I RCCGGY 1 cut(s) 2399
Bse21I CCTNAGG 1 cut(s) 2191
Bse3DI GCAATG 1 cut(s) 3648
BseBI CCWGG 6 cut(s) 1466, 2937, 3252, 3420, 3665, 4026
BseMI GCAATG 1 cut(s) 3648
BseRI GAGGAG 1 cut(s) 2145
BseSI GKGCMC 2 cut(s) 1116, 1274
BseYI CCCAGC 4 cut(s) 2428, 2642, 3465, 3740
BsgI GTGCAG 4 cut(s) 219, 1587, 2531, 3491
Bsh1236I CGCG 1 cut(s) 3729
BshTI ACCGGT 1 cut(s) 2399
BsiHKAI GWGCWC 4 cut(s) 1116, 1437, 2155, 3950
BsiHKCI CYCGRG 1 cut(s) 562
BsiSI CCGG 7 cut(s) 563, 600, 1460, 2400, 2534, 2606, 4011
BslFI GGGAC 2 cut(s) 543, 1008
BsmAI GTCTC 6 cut(s) 486, 1157, 1693, 2340, 3391, 4110
BsmFI GGGAC 2 cut(s) 543, 1008
BsmI GAATGC 1 cut(s) 2931
BsoBI CYCGRG 1 cut(s) 562
Bsp119I TTCGAA 4 cut(s) 460, 1251, 2351, 3347
Bsp1286I GDGCHC 5 cut(s) 1116, 1274, 1437, 2155, 3950
Bsp1407I TGTACA 2 cut(s) 3645, 3899
Bsp19I CCATGG 4 cut(s) 1219, 1989, 2448, 3783
BspACI CCGC 7 cut(s) 74, 413, 757, 1847, 2486, 3285, 3727
BspFNI CGCG 1 cut(s) 3729
BspHI TCATGA 2 cut(s) 3549, 4195
BspLI GGNNCC 3 cut(s) 559, 1024, 3640
BspMAI CTGCAG 4 cut(s) 99, 938, 1104, 1294
BspPI GGATC 4 cut(s) 830, 1332, 1980, 4073
BspT104I TTCGAA 4 cut(s) 460, 1251, 2351, 3347
BsrBI CCGCTC 1 cut(s) 415
BsrDI GCAATG 1 cut(s) 3648
BsrFI RCCGGY 1 cut(s) 2399
BsrGI TGTACA 2 cut(s) 3645, 3899
BssAI RCCGGY 1 cut(s) 2399
BssNAI GTATAC 1 cut(s) 3919
BssNI GRCGYC 2 cut(s) 513, 522
BssSI CACGAG 1 cut(s) 2337
BssT1I CCWWGG 6 cut(s) 1219, 1989, 2448, 3783, 3867, 3922
Bst1107I GTATAC 1 cut(s) 3919
Bst2BI CACGAG 1 cut(s) 2337
Bst2UI CCWGG 6 cut(s) 1466, 2937, 3252, 3420, 3665, 4026
Bst6I CTCTTC 1 cut(s) 3319
BstACI GRCGYC 2 cut(s) 513, 522
BstAPI GCANNNNNTGC 2 cut(s) 1111, 3622
BstAUI TGTACA 2 cut(s) 3645, 3899
BstBAI YACGTR 2 cut(s) 715, 1631
BstBI TTCGAA 4 cut(s) 460, 1251, 2351, 3347
BstC8I GCNNGC 5 cut(s) 757, 1916, 3072, 3484, 3810
BstDSI CCRYGG 4 cut(s) 1219, 1989, 2448, 3783
BstENI CCTNNNNNAGG 2 cut(s) 3033, 3843
BstFNI CGCG 1 cut(s) 3729
BstH2I RGCGCY 1 cut(s) 1037
BstHHI GCGC 3 cut(s) 1036, 1588, 2967
BstMAI GTCTC 6 cut(s) 486, 1157, 1693, 2340, 3391, 4110
BstNI CCWGG 6 cut(s) 1466, 2937, 3252, 3420, 3665, 4026
BstSFI CTRYAG 4 cut(s) 95, 934, 1100, 1290
BstSLI GKGCMC 2 cut(s) 1116, 1274
BstSNI TACGTA 1 cut(s) 1631
BstUI CGCG 1 cut(s) 3729
BstV2I GAAGAC 1 cut(s) 2744
BstX2I RGATCY 1 cut(s) 4065
BstYI RGATCY 1 cut(s) 4065
BstZ17I GTATAC 1 cut(s) 3919
Bsu36I CCTNAGG 1 cut(s) 2191
BtgI CCRYGG 4 cut(s) 1219, 1989, 2448, 3783
BtsI GCAGTG 4 cut(s) 104, 943, 1599, 3978
Cac8I GCNNGC 5 cut(s) 757, 1916, 3072, 3484, 3810
CaiI CAGNNNCTG 1 cut(s) 1160
CciI TCATGA 2 cut(s) 3549, 4195
CfoI GCGC 3 cut(s) 1036, 1588, 2967
Cfr10I RCCGGY 1 cut(s) 2399
Cfr9I CCCGGG 1 cut(s) 562
CseI GACGC 2 cut(s) 430, 511
CspAI ACCGGT 1 cut(s) 2399
DraIII CACNNNGTG 1 cut(s) 1841
EaeI YGGCCR 4 cut(s) 982, 2938, 3780, 3975
Eam1104I CTCTTC 1 cut(s) 3319
EarI CTCTTC 1 cut(s) 3319
Ecl136II GAGCTC 2 cut(s) 1435, 3948
Eco105I TACGTA 1 cut(s) 1631
Eco130I CCWWGG 6 cut(s) 1219, 1989, 2448, 3783, 3867, 3922
Eco24I GRGCYC 2 cut(s) 1437, 3950
Eco32I GATATC 3 cut(s) 931, 2250, 3139
Eco47I GGWCC 7 cut(s) 141, 557, 1022, 1223, 1794, 3639, 4028
Eco47III AGCGCT 1 cut(s) 1035
Eco53kI GAGCTC 2 cut(s) 1435, 3948
Eco57I CTGAAG 7 cut(s) 641, 669, 1226, 1286, 1841, 2555, 3300
Eco81I CCTNAGG 1 cut(s) 2191
Eco88I CYCGRG 1 cut(s) 562
EcoICRI GAGCTC 2 cut(s) 1435, 3948
EcoNI CCTNNNNNAGG 2 cut(s) 3033, 3843
EcoO109I RGGNCCY 2 cut(s) 141, 3480
EcoRI GAATTC 1 cut(s) 2144
EcoRII CCWGG 6 cut(s) 1464, 2935, 3250, 3418, 3663, 4024
EcoRV GATATC 3 cut(s) 931, 2250, 3139
EcoT14I CCWWGG 6 cut(s) 1219, 1989, 2448, 3783, 3867, 3922
EcoT22I ATGCAT 2 cut(s) 961, 2795
EcoT38I GRGCYC 2 cut(s) 1437, 3950
ErhI CCWWGG 6 cut(s) 1219, 1989, 2448, 3783, 3867, 3922
FalI AAGNNNNNCTT 2 cut(s) 2905, 2937
FaqI GGGAC 2 cut(s) 543, 1008
FauI CCCGC 2 cut(s) 764, 3292
FauNDI CATATG 1 cut(s) 1447
FbaI TGATCA 1 cut(s) 2851
FblI GTMKAC 1 cut(s) 3918
FriOI GRGCYC 2 cut(s) 1437, 3950
FspBI CTAG 9 cut(s) 659, 794, 920, 926, 1743, 2748, 3041, 3129, 3533
GlaI GCGC 3 cut(s) 1035, 1587, 2966
GsaI CCCAGC 4 cut(s) 2432, 2646, 3469, 3744
GsuI CTGGAG 2 cut(s) 2706, 3532
HaeII RGCGCY 1 cut(s) 1037
HapII CCGG 7 cut(s) 563, 600, 1460, 2400, 2534, 2606, 4011
HgaI GACGC 2 cut(s) 430, 511
HhaI GCGC 3 cut(s) 1036, 1588, 2967
Hin1I GRCGYC 2 cut(s) 513, 522
Hin6I GCGC 3 cut(s) 1034, 1586, 2965
HinP1I GCGC 3 cut(s) 1034, 1586, 2965
HincII GTYRAC 1 cut(s) 2524
HindII GTYRAC 1 cut(s) 2524
HindIII AAGCTT 3 cut(s) 51, 442, 1521
HinfI GANTC 9 cut(s) 1231, 1825, 2094, 2384, 2631, 2807, 2918, 3362, 4000
HpaII CCGG 7 cut(s) 563, 600, 1460, 2400, 2534, 2606, 4011
HphI GGTGA 5 cut(s) 173, 370, 965, 1449, 3406
Hpy99I CGWCG 1 cut(s) 424
HpyCH4IV ACGT 4 cut(s) 513, 714, 1630, 3538
HpySE526I ACGT 4 cut(s) 513, 714, 1630, 3538
Hsp92I GRCGYC 2 cut(s) 513, 522
HspAI GCGC 3 cut(s) 1034, 1586, 2965
Ksp22I TGATCA 1 cut(s) 2851
LmnI GCTCC 6 cut(s) 4, 233, 420, 1923, 2158, 2594
LweI GCATC 9 cut(s) 478, 906, 946, 1497, 1834, 1948, 1955, 2802, 3034
MaeI CTAG 9 cut(s) 659, 794, 920, 926, 1743, 2748, 3041, 3129, 3533
MaeII ACGT 4 cut(s) 513, 714, 1630, 3538
MaeIII GTNAC 9 cut(s) 220, 650, 938, 1090, 1140, 1406, 2759, 3304, 4083
MbiI CCGCTC 1 cut(s) 415
MfeI CAATTG 5 cut(s) 2895, 2979, 3060, 3676, 4239
MflI RGATCY 1 cut(s) 4065
MhlI GDGCHC 5 cut(s) 1116, 1274, 1437, 2155, 3950
MlsI TGGCCA 4 cut(s) 984, 2940, 3782, 3977
MluNI TGGCCA 4 cut(s) 984, 2940, 3782, 3977
MlyI GAGTC 3 cut(s) 1240, 2640, 2816
MmeI TCCRAC 1 cut(s) 751
Mox20I TGGCCA 4 cut(s) 984, 2940, 3782, 3977
Mph1103I ATGCAT 2 cut(s) 961, 2795
MroXI GAANNNNTTC 2 cut(s) 2144, 4004
MscI TGGCCA 4 cut(s) 984, 2940, 3782, 3977
MslI CAYNNNNRTG 3 cut(s) 854, 1957, 3548
Msp20I TGGCCA 4 cut(s) 984, 2940, 3782, 3977
MspA1I CMGCKG 5 cut(s) 94, 759, 2432, 2488, 2575
MspI CCGG 7 cut(s) 563, 600, 1460, 2400, 2534, 2606, 4011
MunI CAATTG 5 cut(s) 2895, 2979, 3060, 3676, 4239
Mva1269I GAATGC 1 cut(s) 2931
MvaI CCWGG 6 cut(s) 1466, 2937, 3252, 3420, 3665, 4026
MvnI CGCG 1 cut(s) 3729
NciI CCSGG 4 cut(s) 563, 564, 1461, 2607
NcoI CCATGG 4 cut(s) 1219, 1989, 2448, 3783
NdeI CATATG 1 cut(s) 1447
NlaIV GGNNCC 3 cut(s) 559, 1024, 3640
NmeAIII GCCGAG 1 cut(s) 412
NmuCI GTSAC 4 cut(s) 938, 1090, 1140, 4083
NsiI ATGCAT 2 cut(s) 961, 2795
NspV TTCGAA 4 cut(s) 460, 1251, 2351, 3347
PagI TCATGA 2 cut(s) 3549, 4195
PcsI WCGNNNNNNNCGW 3 cut(s) 165, 519, 711
PctI GAATGC 1 cut(s) 2931
PdmI GAANNNNTTC 2 cut(s) 2144, 4004
PfeI GAWTC 6 cut(s) 1825, 2094, 2384, 2918, 3362, 4000
PflMI CCANNNNNTGG 1 cut(s) 2449
PinAI ACCGGT 1 cut(s) 2399
PleI GAGTC 3 cut(s) 1239, 2639, 2815
PpsI GAGTC 3 cut(s) 1239, 2639, 2815
Ppu21I YACGTR 2 cut(s) 715, 1631
PpuMI RGGWCCY 1 cut(s) 141
PshBI ATTAAT 1 cut(s) 4136
PsiI TTATAA 1 cut(s) 3357
Psp124BI GAGCTC 2 cut(s) 1437, 3950
Psp5II RGGWCCY 1 cut(s) 141
Psp6I CCWGG 6 cut(s) 1464, 2935, 3250, 3418, 3663, 4024
PspFI CCCAGC 4 cut(s) 2428, 2642, 3465, 3740
PspGI CCWGG 6 cut(s) 1464, 2935, 3250, 3418, 3663, 4024
PspN4I GGNNCC 3 cut(s) 559, 1024, 3640
PspPPI RGGWCCY 1 cut(s) 141
PstI CTGCAG 4 cut(s) 99, 938, 1104, 1294
PstNI CAGNNNCTG 1 cut(s) 1160
PsuI RGATCY 1 cut(s) 4065
PvuII CAGCTG 3 cut(s) 94, 2432, 2575
RseI CAYNNNNRTG 3 cut(s) 854, 1957, 3548
SacI GAGCTC 2 cut(s) 1437, 3950
SchI GAGTC 3 cut(s) 1240, 2640, 2816
SduI GDGCHC 5 cut(s) 1116, 1274, 1437, 2155, 3950
SfaNI GCATC 9 cut(s) 478, 906, 946, 1497, 1834, 1948, 1955, 2802, 3034
SfcI CTRYAG 4 cut(s) 95, 934, 1100, 1290
SfiI GGCCNNNNNGGCC 1 cut(s) 2946
SfuI TTCGAA 4 cut(s) 460, 1251, 2351, 3347
SinI GGWCC 7 cut(s) 141, 557, 1022, 1223, 1794, 3639, 4028
SmaI CCCGGG 1 cut(s) 564
SmiMI CAYNNNNRTG 3 cut(s) 854, 1957, 3548
SmlI CTYRAG 2 cut(s) 483, 3424
SmoI CTYRAG 2 cut(s) 483, 3424
SnaBI TACGTA 1 cut(s) 1631
SsiI CCGC 7 cut(s) 74, 413, 757, 1847, 2486, 3285, 3727
SspI AATATT 3 cut(s) 3015, 3236, 3660
SspMI CTAG 9 cut(s) 659, 794, 920, 926, 1743, 2748, 3041, 3129, 3533
SstI GAGCTC 2 cut(s) 1437, 3950
StyI CCWWGG 6 cut(s) 1219, 1989, 2448, 3783, 3867, 3922
TaiI ACGT 4 cut(s) 516, 717, 1633, 3541
TaqII GACCGA 2 cut(s) 1010, 4045
TatI WGTACW 5 cut(s) 400, 2514, 3645, 3747, 3899
TauI GCSGC 2 cut(s) 415, 2488
TfiI GAWTC 6 cut(s) 1825, 2094, 2384, 2918, 3362, 4000
TseFI GTSAC 4 cut(s) 938, 1090, 1140, 4083
Tsp45I GTSAC 4 cut(s) 938, 1090, 1140, 4083
TspGWI ACGGA 1 cut(s) 2138
TspMI CCCGGG 1 cut(s) 562
Van91I CCANNNNNTGG 1 cut(s) 2449
VneI GTGCAC 1 cut(s) 1112
VpaK11BI GGWCC 7 cut(s) 141, 557, 1022, 1223, 1794, 3639, 4028
VspI ATTAAT 1 cut(s) 4136
XagI CCTNNNNNAGG 2 cut(s) 3033, 3843
XapI RAATTY 7 cut(s) 1382, 1558, 2144, 2202, 2870, 4035, 4282
XcmI CCANNNNNNNNNTGG 1 cut(s) 3462
XmaI CCCGGG 1 cut(s) 562
XmiI GTMKAC 1 cut(s) 3918
XmnI GAANNNNTTC 2 cut(s) 2144, 4004
XspI CTAG 9 cut(s) 659, 794, 920, 926, 1743, 2748, 3041, 3129, 3533
ZraI GACGTC 1 cut(s) 514
Zsp2I ATGCAT 2 cut(s) 961, 2795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.