Rorug02G0539500

pleiotropic drug resistance protein 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000472
Physical Location & Seq
Forward (+)
66905680 .. 66908749
3070 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0539500.1

Sequence Viewer

Length: 2505 bp
ATGCCAGTATCTCTAGTTCTCTACCTTGTCTGCAAATATTTCTTTGCAGCCATGGAGTCCAAAACAAACCCAAAATTCTTGCTTTCCTTGCTTTTCTTAAGCCTATGCCTCAAGTCCCATATCTGCTTTGGAGCTGACACCATCACAGCAAACCAGTCTCTCTGTGGTGACCAAACCATTGTTTCAGCTGGTGGGAAATTTGAGCTGGGTTTCTTCAAGGCAGGTAATACTTCAAACTACTACATAGGCATGTGGTACTACAAGACAATAGTAACACAGCAAACCATAGTCTGGGTGGCCAATAGGGAGCAGCCAGTCTCTGATAGATTTTCTTCAGAGTTGAGAATCTCAGATGGTAATCTGGTGCTGTTTAATGAGTCCAAAACCCCAGTTTGGTCCACAGATGTCAGTTCAAGTTCAGCCTCTTCTACACGTGTAGTTCTTTTAGATAATGGAAACCTTGTGCTAAGAGCTGGGTCTAATTTATCTTTGCCTTTATGGCAAAGTTTTGATTATCCAGCTGATACTTGGCTACCAGAAGCTAGAATTGGGTTCAACACTGTTACAAACCGAACCCAAGTCCTGACTTCATGGAAGAATTTTCAGGATCCTGCACCGGGTATTTACTCTCTCGTGCTTGACCCGACTGGAAGTGATGCATATTTCTCACTGTGGAATAGGTCTAAGCAGTATTGGACTAGTGGAGCTTGGGATGCAAAGAGTCATATTTTCAGTTTGGTTCCTGAGATGAGGTATAAGTTAATCTACAACTTCAGCTATGTTAAAAACAAAAATGAGAGCTACTTCACCTATTCTGTTTATGATCCTAGCATCACATCTCGATTCATAATGGATGTATCGGGTCAGGTTAAGCAAGAGTCCTGGTTGAAATCTCAGGGATGGAACATGTTTTGGTCTCAGCCGTGGAAACTATGTGAGGTTTATTCCTATTGTGGTGCATTTGGCAGATGCAATGAGAAGTCCTTGCCCTTCTGTAATTGTTTATATGGTTTTGAGCCGAAATTGAAGGTGGATTGGAATTCTCAGGTTTATTCTGGTGGCTGCAAAAGACGGACTGTGCTGAATTTTGCTAATGCTACTACCAATAATGGGAAGCAAGACCGGTTTCTCGAAATGCCTAGCATGTCTTTGCCTGATAGTAAAGTGTCTGTAGAGGCTGGGAGTACTGCGCAATGTGAATCAATCTGCTTAAGTAACCGCTCTTGCACTGCTTATGCTTATCATAGCAATGGATGTTCAATATGGATTGGAGATATCTTGAATCTGCAACAACACACAGCAGATGACGGTGATGGAAAAACTTTTTACCTCAGACTTGCAGCTTCCGAGTTTAAGGATCCAAAAAGTAAAAAGGGATTGATTATCGGTGTTGCAGTAGGCTCAGCTGTTGGGATAGCAGCTCTTGTAGGCCTTATTGTGGTTAAAGTGTTGAGAAACAGAAATAGAGTGATCGGAAAATCAGTGGAGGGTCCATTGGTGTCATTTGAGTATAGAGATCTTCAAGAAGTAACAAAGAACTTCTTGGAGAAATTGGGGGGAGGAGGTTTTGGTTCTGTCTTCAAGGGCACAATGCCTGATTCATCAGTCATAGCAGTGAAGAAGCTCGAAAGTGTTAGCCAAGGAGAGAAGCAATTCAGAACAGAAGTCAGCACAATTGGGACAATCCAACATGTCAATCTTGTTCGGCTTCGTGGGTTCTGCTCTGAAGGTGCCAAGAGGTTGTTGGTCTATGATTACATGCCTAATGGATCTCTGGATGCTCACCTTTTCAATCATCCAAGCGATGTTTTAGAGTGGAATACAAGATACCAAATTGCTCTGGGTACAGCAAGAGGGTTGGCTTATCTTCATGAGAAATGTAGAGATTGTATCATACATTGCGACATAAAGCCAGAAAACATTCTCCTAGATACTGAACTTTGTCCCAAAGTTGCAGACTTTGGCCTGGCAAAGCTTGTTGGAAGAGAGTTCAGCCGAGTCCTGACCACAATGAGAGGCACACGAGGATATCTTGCACCAGAGTGGATTTCAGGAGTGGCAGTCACAGCAAAAGCTGATGTTTACAGCTACGGAATGATGCTCTTCGAAATTGTATCAGGAAGACGAAACACTGAGCCATCTGAAGACGGCAAAGTTAGATTCTTCCCAACTCGGGCTGCTGGCTTGATCACAAGTAACGAAGAAGGCGATGTGCTGAGCCTATTGGACCCGAAATTAGGGAGAAATGGTGATGTAGAAGAGATCATGAGGGTTCTTAGAGTTGCTTGCTGGTGTGTCCAAGATGATGAGGCTCATAGACCATCAATGGGTCAAGTGGTGCAGATTCTCGAGGGGGTTTTGAATGTGAACTTGCCCTCAGTTCCAAGATCTCTCCAACTCTTTGGAGATAGCCAGCACATAGTTTTCTTCACCGAGTCATCTGCAAGCCAGAGCTCACATGCACCAAGCAATAATACATCAACAGCTTCATCGATCTCAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

834

Amino Acids

92.76

Weight (kDa)

8.08

Isoelectric Point (pI)

37.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 95 - 199 5e-34 D-mannose binding lectin
S_locus_glycop PF00954 232 - 341 4.4e-27 S-locus glycoprotein domain
PAN_2 PF08276 372 - 428 7.1e-14 PAN-like domain
PK_Tyr_Ser-Thr PF07714 514 - 782 2e-40 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 515 - 781 2e-46 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000472)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15230 AT4G15230 AT4G15230 AT4G15230 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15236
fragaria_vesca FvH4_4g21360 FvH4_4g21360 FvH4_4g21390 FvH4_6g49431 FvH4_6g49450
malus_domestica MD09G1044300.v1.1 MD16G1137900.v1.1 MD16G1138000.v1.1
prunus_persica Prupe.1G106800_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.3G273300_v2.0.a1
pyrus_communis pycom111g03460 pycom16g12210
rosa_chinensis RchiOBHm_Chr2g0169331 RchiOBHm_Chr2g0169361 RchiOBHm_Chr2g0169371 RchiOBHm_Chr2g0169381 RchiOBHm_Chr2g0169411 RchiOBHm_Chr4g0427141 RchiOBHm_Chr4g0427321
rosa_laevigata RLG00000007252 RLG00000007258 RLG00000013974 RLG00000021856 RLG00000021857 RLG00000021858 RLG00000021859
rosa_multiflora Rmu_sc0000648.1_g000007 Rmu_sc0000648.1_g000009 Rmu_sc0003813.1_g000016 Rmu_sc0009165.1_g000008 Rmu_sc0022554.1_g000001
rosa_roxburghii Rroxscaffold_2G00082200 Rroxscaffold_2G00082230 Rroxscaffold_5G00368960 Rroxscaffold_5G00369010 Rroxscaffold_7G00200390
rosa_rugosa Rorug02G0539500 Rorug02G0539600 Rorug02G0539700 Rorug04G0216400 Rorug04G0216500 Rorug04G0216500 Rorug04G0216600
rosa_samantha Rh2AG610800 Rh2AG611000 Rh2AG611100 Rh2CG592800 Rh2CG593000 Rh2CG593200 Rh2DG634900 Rh2DG635100 Rh2DG635200 Rh2DG635400 Rh4AG272200 Rh4AG272900 Rh4DG275600 Rh4DG276000
rosa_wichuraiana Rw2G050640 Rw2G050660 Rw2G050670 Rw2G050680 Rw4G023610 Rw4G023680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1191
Acc36I ACCTGC 1 cut(s) 212
AccB1I GGYRCC 1 cut(s) 1730
AccB7I CCANNNNNTGG 1 cut(s) 291
AccBSI CCGCTC 1 cut(s) 1221
AciI CCGC 1 cut(s) 1219
AclWI GGATC 6 cut(s) 602, 615, 818, 1352, 1365, 1777
AcoI YGGCCR 1 cut(s) 297
AcsI RAATTY 5 cut(s) 74, 197, 598, 1039, 1084
AcuI CTGAAG 4 cut(s) 318, 757, 1746, 2163
AcvI CACGTG 1 cut(s) 434
AfaI GTAC 3 cut(s) 257, 1186, 1846
AfiI CCNNNNNNNGG 8 cut(s) 291, 393, 617, 1110, 1438, 2173, 2237, 2327
AflII CTTAAG 2 cut(s) 97, 1210
AflIII ACRYGT 4 cut(s) 431, 433, 906, 1690
AgeI ACCGGT 1 cut(s) 1122
AhlI ACTAGT 1 cut(s) 698
AjnI CCWGG 2 cut(s) 881, 1965
AjuI GAANNNNNNNTTGG 2 cut(s) 531, 563
AleI CACNNNNGTG 1 cut(s) 2041
Alw21I GWGCWC 1 cut(s) 2456
Alw26I GTCTC 3 cut(s) 162, 322, 921
AlwI GGATC 6 cut(s) 602, 615, 818, 1352, 1365, 1777
AlwNI CAGNNNCTG 1 cut(s) 320
Ama87I CYCGRG 2 cut(s) 2172, 2348
AoxI GGCC 3 cut(s) 297, 1429, 1963
ApeKI GCWGC 6 cut(s) 47, 310, 1062, 1340, 1418, 2177
ApoI RAATTY 5 cut(s) 74, 197, 598, 1039, 1084
AsiGI ACCGGT 1 cut(s) 1122
Asp700I GAANNNNTTC 2 cut(s) 1652, 1920
AspLEI GCGC 1 cut(s) 1192
AspS9I GGNCC 3 cut(s) 396, 1490, 2227
AsuC2I CCSGG 1 cut(s) 618
AsuHPI GGTGA 6 cut(s) 179, 799, 1322, 1775, 2261, 2422
AsuII TTCGAA 1 cut(s) 2106
AvaI CYCGRG 2 cut(s) 2172, 2348
AvaII GGWCC 3 cut(s) 396, 1490, 2227
BaeGI GKGCMC 1 cut(s) 1589
BalI TGGCCA 1 cut(s) 299
BamHI GGATCC 2 cut(s) 607, 1357
BanI GGYRCC 1 cut(s) 1730
BanII GRGCYC 1 cut(s) 2456
BauI CACGAG 2 cut(s) 632, 2022
BbrPI CACGTG 1 cut(s) 434
BbsI GAAGAC 3 cut(s) 1570, 2128, 2151
Bbv12I GWGCWC 1 cut(s) 2456
BbvI GCAGC 6 cut(s) 59, 322, 1049, 1352, 1430, 2164
BccI CCATC 6 cut(s) 149, 347, 894, 1307, 2146, 2329
BceAI ACGGC 2 cut(s) 907, 2164
BcgI CGANNNNNNTGC 2 cut(s) 2423, 2457
BciT130I CCWGG 2 cut(s) 883, 1967
BclI TGATCA 1 cut(s) 2187
BcnI CCSGG 1 cut(s) 618
BcoDI GTCTC 3 cut(s) 162, 322, 921
BcuI ACTAGT 1 cut(s) 698
BfaI CTAG 6 cut(s) 14, 543, 699, 828, 1140, 1928
BfmI CTRYAG 1 cut(s) 1170
BfrI CTTAAG 2 cut(s) 97, 1210
BfuAI ACCTGC 1 cut(s) 212
BglI GCCNNNNNGGC 1 cut(s) 499
BglII AGATCT 2 cut(s) 1516, 2387
BisI GCNGC 6 cut(s) 48, 311, 1063, 1341, 1419, 2178
BlpI GCTNAGC 2 cut(s) 1402, 2216
BlsI GCNGC 6 cut(s) 49, 312, 1064, 1342, 1420, 2179
BmcAI AGTACT 1 cut(s) 1186
Bme1390I CCNGG 3 cut(s) 618, 883, 1967
Bme18I GGWCC 3 cut(s) 396, 1490, 2227
BmeT110I CYCGRG 2 cut(s) 2172, 2348
BmgT120I GGNCC 3 cut(s) 396, 1490, 2227
BmiI GGNNCC 6 cut(s) 609, 741, 1359, 1491, 1732, 2229
BmrFI CCNGG 3 cut(s) 618, 883, 1967
BmrI ACTGGG 1 cut(s) 383
BmsI GCATC 6 cut(s) 646, 703, 840, 959, 1768, 2088
BmuI ACTGGG 1 cut(s) 383
BpiI GAAGAC 3 cut(s) 1570, 2128, 2151
Bpu1102I GCTNAGC 2 cut(s) 1402, 2216
Bpu14I TTCGAA 1 cut(s) 2106
BpuEI CTTGAG 1 cut(s) 95
BpuMI CCSGG 1 cut(s) 618
Bsa29I ATCGAT 1 cut(s) 2492
BsaAI YACGTR 1 cut(s) 434
BsaBI GATNNNNATC 1 cut(s) 357
BsaI GGTCTC 1 cut(s) 921
BsaJI CCNNGG 3 cut(s) 51, 923, 1639
BsaWI WCCGGW 1 cut(s) 1122
BsaXI ACNNNNNCTCC 4 cut(s) 2046, 2076, 2233, 2263
Bsc4I CCNNNNNNNGG 8 cut(s) 291, 393, 617, 1110, 1438, 2173, 2237, 2327
Bse118I RCCGGY 1 cut(s) 1122
Bse1I ACTGG 5 cut(s) 5, 154, 314, 389, 652
Bse3DI GCAATG 4 cut(s) 979, 1199, 1255, 1897
Bse8I GATNNNNATC 1 cut(s) 357
BseBI CCWGG 2 cut(s) 883, 1967
BseCI ATCGAT 1 cut(s) 2492
BseDI CCNNGG 3 cut(s) 51, 923, 1639
BseGI GGATG 6 cut(s) 718, 859, 905, 1259, 1783, 1795
BseJI GATNNNNATC 1 cut(s) 357
BseLI CCNNNNNNNGG 8 cut(s) 291, 393, 617, 1110, 1438, 2173, 2237, 2327
BseMI GCAATG 4 cut(s) 979, 1199, 1255, 1897
BseNI ACTGG 5 cut(s) 5, 154, 314, 389, 652
BseRI GAGGAG 1 cut(s) 1575
BseSI GKGCMC 1 cut(s) 1589
BseXI GCAGC 6 cut(s) 59, 322, 1049, 1352, 1430, 2164
BseYI CCCAGC 3 cut(s) 205, 473, 1178
BsgI GTGCAG 2 cut(s) 597, 2360
BshFI GGCC 3 cut(s) 299, 1431, 1965
BshNI GGYRCC 1 cut(s) 1730
BshTI ACCGGT 1 cut(s) 1122
BshVI ATCGAT 1 cut(s) 2492
BsiHKAI GWGCWC 1 cut(s) 2456
BsiHKCI CYCGRG 2 cut(s) 2172, 2348
BsiSI CCGG 2 cut(s) 617, 1123
BslFI GGGAC 3 cut(s) 100, 1693, 1929
BslI CCNNNNNNNGG 8 cut(s) 291, 393, 617, 1110, 1438, 2173, 2237, 2327
BsmAI GTCTC 3 cut(s) 162, 322, 921
BsmFI GGGAC 3 cut(s) 100, 1693, 1929
BsnI GGCC 3 cut(s) 299, 1431, 1965
Bso31I GGTCTC 1 cut(s) 921
BsoBI CYCGRG 2 cut(s) 2172, 2348
Bsp119I TTCGAA 1 cut(s) 2106
Bsp1286I GDGCHC 2 cut(s) 1589, 2456
Bsp1720I GCTNAGC 2 cut(s) 1402, 2216
Bsp19I CCATGG 1 cut(s) 51
BspACI CCGC 1 cut(s) 1219
BspANI GGCC 3 cut(s) 299, 1431, 1965
BspDI ATCGAT 1 cut(s) 2492
BspHI TCATGA 2 cut(s) 1870, 2265
BspLI GGNNCC 6 cut(s) 609, 741, 1359, 1491, 1732, 2229
BspMI ACCTGC 1 cut(s) 212
BspPI GGATC 6 cut(s) 602, 615, 818, 1352, 1365, 1777
BspQI GCTCTTC 1 cut(s) 2108
BspT104I TTCGAA 1 cut(s) 2106
BspT107I GGYRCC 1 cut(s) 1730
BspTI CTTAAG 2 cut(s) 97, 1210
BspTNI GGTCTC 1 cut(s) 921
BsrBI CCGCTC 1 cut(s) 1221
BsrDI GCAATG 4 cut(s) 979, 1199, 1255, 1897
BsrFI RCCGGY 1 cut(s) 1122
BsrI ACTGG 5 cut(s) 5, 154, 314, 389, 652
BssAI RCCGGY 1 cut(s) 1122
BssECI CCNNGG 3 cut(s) 51, 923, 1639
BssSI CACGAG 2 cut(s) 632, 2022
BssT1I CCWWGG 2 cut(s) 51, 1639
Bst2BI CACGAG 2 cut(s) 632, 2022
Bst2UI CCWGG 2 cut(s) 883, 1967
Bst4CI ACNGT 4 cut(s) 562, 672, 1078, 1310
Bst6I CTCTTC 4 cut(s) 430, 1978, 2108, 2253
BstAFI CTTAAG 2 cut(s) 97, 1210
BstBAI YACGTR 1 cut(s) 434
BstBI TTCGAA 1 cut(s) 2106
BstC8I GCNNGC 4 cut(s) 2182, 2287, 2414, 2446
BstDSI CCRYGG 2 cut(s) 51, 923
BstEII GGTNACC 1 cut(s) 167
BstF5I GGATG 6 cut(s) 718, 859, 905, 1259, 1783, 1795
BstHHI GCGC 1 cut(s) 1192
BstMAI GTCTC 3 cut(s) 162, 322, 921
BstMWI GCNNNNNNNGC 4 cut(s) 88, 499, 713, 2066
BstNI CCWGG 2 cut(s) 883, 1967
BstNSI RCATGY 6 cut(s) 253, 910, 1147, 1694, 1762, 2462
BstPI GGTNACC 1 cut(s) 167
BstSCI CCNGG 3 cut(s) 616, 881, 1965
BstSFI CTRYAG 1 cut(s) 1170
BstSLI GKGCMC 1 cut(s) 1589
BstV1I GCAGC 6 cut(s) 59, 322, 1049, 1352, 1430, 2164
BstV2I GAAGAC 3 cut(s) 1570, 2128, 2151
BstX2I RGATCY 5 cut(s) 607, 1357, 1516, 1769, 2387
BstXI CCANNNNNNTGG 1 cut(s) 2402
BstYI RGATCY 5 cut(s) 607, 1357, 1516, 1769, 2387
Bsu15I ATCGAT 1 cut(s) 2492
BsuRI GGCC 3 cut(s) 299, 1431, 1965
BsuTUI ATCGAT 1 cut(s) 2492
BtgI CCRYGG 2 cut(s) 51, 923
BtgZI GCGATG 2 cut(s) 1818, 2223
BtsCI GGATG 6 cut(s) 718, 859, 905, 1259, 1783, 1795
BtsI GCAGTG 2 cut(s) 1227, 1620
BtsIMutI CAGTG 6 cut(s) 558, 668, 1227, 1488, 1620, 2130
BveI ACCTGC 1 cut(s) 212
Cac8I GCNNGC 4 cut(s) 2182, 2287, 2414, 2446
CaiI CAGNNNCTG 1 cut(s) 320
CciI TCATGA 2 cut(s) 1870, 2265
CfoI GCGC 1 cut(s) 1192
Cfr10I RCCGGY 1 cut(s) 1122
Cfr13I GGNCC 3 cut(s) 396, 1490, 2227
ClaI ATCGAT 1 cut(s) 2492
Csp6I GTAC 3 cut(s) 256, 1185, 1845
CspAI ACCGGT 1 cut(s) 1122
CviQI GTAC 3 cut(s) 256, 1185, 1845
EaeI YGGCCR 1 cut(s) 297
Eam1104I CTCTTC 4 cut(s) 430, 1978, 2108, 2253
EarI CTCTTC 4 cut(s) 430, 1978, 2108, 2253
Ecl136II GAGCTC 1 cut(s) 2454
Eco130I CCWWGG 2 cut(s) 51, 1639
Eco147I AGGCCT 1 cut(s) 1431
Eco24I GRGCYC 1 cut(s) 2456
Eco31I GGTCTC 1 cut(s) 921
Eco32I GATATC 2 cut(s) 1276, 2030
Eco47I GGWCC 3 cut(s) 396, 1490, 2227
Eco53kI GAGCTC 1 cut(s) 2454
Eco57I CTGAAG 4 cut(s) 318, 757, 1746, 2163
Eco72I CACGTG 1 cut(s) 434
Eco88I CYCGRG 2 cut(s) 2172, 2348
Eco91I GGTNACC 1 cut(s) 167
EcoICRI GAGCTC 1 cut(s) 2454
EcoO65I GGTNACC 1 cut(s) 167
EcoRI GAATTC 1 cut(s) 1039
EcoRII CCWGG 2 cut(s) 881, 1965
EcoRV GATATC 2 cut(s) 1276, 2030
EcoT14I CCWWGG 2 cut(s) 51, 1639
EcoT22I ATGCAT 1 cut(s) 661
EcoT38I GRGCYC 1 cut(s) 2456
ErhI CCWWGG 2 cut(s) 51, 1639
FalI AAGNNNNNCTT 2 cut(s) 1526, 1558
FaqI GGGAC 3 cut(s) 100, 1693, 1929
FbaI TGATCA 1 cut(s) 2187
Fnu4HI GCNGC 6 cut(s) 48, 311, 1063, 1341, 1419, 2178
FokI GGATG 6 cut(s) 725, 866, 912, 1266, 1782, 1790
FriOI GRGCYC 1 cut(s) 2456
Fsp4HI GCNGC 6 cut(s) 48, 311, 1063, 1341, 1419, 2178
FspBI CTAG 6 cut(s) 14, 543, 699, 828, 1140, 1928
FspI TGCGCA 1 cut(s) 1191
GlaI GCGC 1 cut(s) 1191
GluI GCNGC 6 cut(s) 48, 311, 1063, 1341, 1419, 2178
GsaI CCCAGC 3 cut(s) 209, 477, 1182
HaeIII GGCC 3 cut(s) 299, 1431, 1965
HapII CCGG 2 cut(s) 617, 1123
HhaI GCGC 1 cut(s) 1192
Hin6I GCGC 1 cut(s) 1190
HinP1I GCGC 1 cut(s) 1190
HindIII AAGCTT 1 cut(s) 1973
HpaII CCGG 2 cut(s) 617, 1123
HphI GGTGA 6 cut(s) 179, 799, 1322, 1775, 2261, 2422
Hpy166II GTNNAC 3 cut(s) 399, 2083, 2368
Hpy8I GTNNAC 3 cut(s) 399, 2083, 2368
HpyAV CCTTC 4 cut(s) 1000, 1021, 1721, 2198
HpyCH4III ACNGT 4 cut(s) 562, 672, 1078, 1310
HpyCH4IV ACGT 1 cut(s) 433
HpyF10VI GCNNNNNNNGC 4 cut(s) 88, 499, 713, 2066
HpySE526I ACGT 1 cut(s) 433
HspAI GCGC 1 cut(s) 1190
Ksp22I TGATCA 1 cut(s) 2187
LguI GCTCTTC 1 cut(s) 2108
LmnI GCTCC 3 cut(s) 131, 307, 704
Lsp1109I GCAGC 6 cut(s) 59, 322, 1049, 1352, 1430, 2164
LweI GCATC 6 cut(s) 646, 703, 840, 959, 1768, 2088
MaeI CTAG 6 cut(s) 14, 543, 699, 828, 1140, 1928
MaeII ACGT 1 cut(s) 433
MaeIII GTNAC 7 cut(s) 167, 271, 562, 1214, 1528, 2062, 2195
MbiI CCGCTC 1 cut(s) 1221
MfeI CAATTG 1 cut(s) 1674
MflI RGATCY 5 cut(s) 607, 1357, 1516, 1769, 2387
MhlI GDGCHC 2 cut(s) 1589, 2456
MlsI TGGCCA 1 cut(s) 299
MluNI TGGCCA 1 cut(s) 299
MlyI GAGTC 6 cut(s) 65, 386, 730, 887, 2007, 2444
MmeI TCCRAC 3 cut(s) 1711, 1960, 2419
Mox20I TGGCCA 1 cut(s) 299
Mph1103I ATGCAT 1 cut(s) 661
MroXI GAANNNNTTC 2 cut(s) 1652, 1920
MscI TGGCCA 1 cut(s) 299
MseI TTAA 8 cut(s) 98, 372, 761, 783, 870, 1211, 1353, 1443
MslI CAYNNNNRTG 4 cut(s) 248, 1248, 1613, 2041
Msp20I TGGCCA 1 cut(s) 299
MspA1I CMGCKG 3 cut(s) 188, 521, 1406
MspCI CTTAAG 2 cut(s) 97, 1210
MspI CCGG 2 cut(s) 617, 1123
MspR9I CCNGG 3 cut(s) 618, 883, 1967
MunI CAATTG 1 cut(s) 1674
MvaI CCWGG 2 cut(s) 883, 1967
MwoI GCNNNNNNNGC 4 cut(s) 88, 499, 713, 2066
NciI CCSGG 1 cut(s) 618
NcoI CCATGG 1 cut(s) 51
NlaIV GGNNCC 6 cut(s) 609, 741, 1359, 1491, 1732, 2229
NmeAIII GCCGAG 1 cut(s) 2021
NmuCI GTSAC 2 cut(s) 167, 2062
NsbI TGCGCA 1 cut(s) 1191
NsiI ATGCAT 1 cut(s) 661
NspI RCATGY 6 cut(s) 253, 910, 1147, 1694, 1762, 2462
NspV TTCGAA 1 cut(s) 2106
OliI CACNNNNGTG 1 cut(s) 2041
PaeR7I CTCGAG 1 cut(s) 2348
PagI TCATGA 2 cut(s) 1870, 2265
PceI AGGCCT 1 cut(s) 1431
PciI ACATGT 2 cut(s) 906, 1690
PciSI GCTCTTC 1 cut(s) 2108
PcsI WCGNNNNNNNCGW 1 cut(s) 2205
PdmI GAANNNNTTC 2 cut(s) 1652, 1920
PfeI GAWTC 7 cut(s) 345, 843, 1199, 1282, 1598, 2160, 2344
PflMI CCANNNNNTGG 1 cut(s) 291
PinAI ACCGGT 1 cut(s) 1122
PkrI GCNGC 6 cut(s) 49, 312, 1064, 1342, 1420, 2179
PleI GAGTC 6 cut(s) 64, 385, 729, 886, 2006, 2443
PmaCI CACGTG 1 cut(s) 434
PmlI CACGTG 1 cut(s) 434
PpsI GAGTC 6 cut(s) 64, 385, 729, 886, 2006, 2443
Ppu21I YACGTR 1 cut(s) 434
PscI ACATGT 2 cut(s) 906, 1690
Psp124BI GAGCTC 1 cut(s) 2456
Psp6I CCWGG 2 cut(s) 881, 1965
PspCI CACGTG 1 cut(s) 434
PspEI GGTNACC 1 cut(s) 167
PspFI CCCAGC 3 cut(s) 205, 473, 1178
PspGI CCWGG 2 cut(s) 881, 1965
PspN4I GGNNCC 6 cut(s) 609, 741, 1359, 1491, 1732, 2229
PspPI GGNCC 3 cut(s) 396, 1490, 2227
PsrI GAACNNNNNNTAC 1 cut(s) 32
PstNI CAGNNNCTG 1 cut(s) 320
PsuI RGATCY 5 cut(s) 607, 1357, 1516, 1769, 2387
PvuII CAGCTG 3 cut(s) 188, 521, 1406
RsaI GTAC 3 cut(s) 257, 1186, 1846
RsaNI GTAC 3 cut(s) 256, 1185, 1845
RseI CAYNNNNRTG 4 cut(s) 248, 1248, 1613, 2041
SacI GAGCTC 1 cut(s) 2456
SapI GCTCTTC 1 cut(s) 2108
SaqAI TTAA 8 cut(s) 98, 372, 761, 783, 870, 1211, 1353, 1443
SatI GCNGC 6 cut(s) 48, 311, 1063, 1341, 1419, 2178
Sau96I GGNCC 3 cut(s) 396, 1490, 2227
ScaI AGTACT 1 cut(s) 1186
SchI GAGTC 6 cut(s) 65, 386, 730, 887, 2007, 2444
ScrFI CCNGG 3 cut(s) 618, 883, 1967
SduI GDGCHC 2 cut(s) 1589, 2456
SfaNI GCATC 6 cut(s) 646, 703, 840, 959, 1768, 2088
SfcI CTRYAG 1 cut(s) 1170
Sfr274I CTCGAG 1 cut(s) 2348
SfuI TTCGAA 1 cut(s) 2106
SinI GGWCC 3 cut(s) 396, 1490, 2227
SlaI CTCGAG 1 cut(s) 2348
SmiMI CAYNNNNRTG 4 cut(s) 248, 1248, 1613, 2041
SmlI CTYRAG 4 cut(s) 97, 110, 1210, 2348
SmoI CTYRAG 4 cut(s) 97, 110, 1210, 2348
SpeI ACTAGT 1 cut(s) 698
SseBI AGGCCT 1 cut(s) 1431
SsiI CCGC 1 cut(s) 1219
SspI AATATT 1 cut(s) 38
SspMI CTAG 6 cut(s) 14, 543, 699, 828, 1140, 1928
SstI GAGCTC 1 cut(s) 2456
StuI AGGCCT 1 cut(s) 1431
StyD4I CCNGG 3 cut(s) 616, 881, 1965
StyI CCWWGG 2 cut(s) 51, 1639
TaaI ACNGT 4 cut(s) 562, 672, 1078, 1310
TaiI ACGT 1 cut(s) 436
TaqI TCGA 6 cut(s) 841, 1131, 1626, 2106, 2349, 2492
TatI WGTACW 1 cut(s) 1184
TfiI GAWTC 7 cut(s) 345, 843, 1199, 1282, 1598, 2160, 2344
Tru1I TTAA 8 cut(s) 98, 372, 761, 783, 870, 1211, 1353, 1443
Tru9I TTAA 8 cut(s) 98, 372, 761, 783, 870, 1211, 1353, 1443
TscAI CASTG 6 cut(s) 565, 675, 1234, 1488, 1620, 2137
TseFI GTSAC 2 cut(s) 167, 2062
TseI GCWGC 6 cut(s) 47, 310, 1062, 1340, 1418, 2177
Tsp45I GTSAC 2 cut(s) 167, 2062
TspDTI ATGAA 5 cut(s) 579, 835, 1590, 1859, 2478
TspGWI ACGGA 2 cut(s) 1087, 2106
TspRI CASTG 6 cut(s) 565, 675, 1234, 1488, 1620, 2137
Van91I CCANNNNNTGG 1 cut(s) 291
Vha464I CTTAAG 2 cut(s) 97, 1210
VpaK11BI GGWCC 3 cut(s) 396, 1490, 2227
XapI RAATTY 5 cut(s) 74, 197, 598, 1039, 1084
XceI RCATGY 6 cut(s) 253, 910, 1147, 1694, 1762, 2462
XcmI CCANNNNNNNNNTGG 5 cut(s) 125, 161, 292, 525, 1741
XhoI CTCGAG 1 cut(s) 2348
XmnI GAANNNNTTC 2 cut(s) 1652, 1920
XspI CTAG 6 cut(s) 14, 543, 699, 828, 1140, 1928
ZrmI AGTACT 1 cut(s) 1186
Zsp2I ATGCAT 1 cut(s) 661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.