RLG00000013974

pleiotropic drug resistance protein 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000472
Physical Location & Seq
Reverse (-)
44944069 .. 44953971
9903 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013974

Sequence Viewer

Length: 4509 bp
ATGGATGCTACAGAGATAGGAAGAAGTTTGAGCACAACATCAAAGCTAGGAGCTCAGGAACGGCGTTTCTTAATTGACAAACTTATCAAAGAAATAGGAGGAGATAACGAACGCCTGTTAAAGAAACTTAGAGAACGAATAGACAGAGTGGGACTAAAATTGCCGACTGTGGAAGTACGGTACAAAAATCTGTTTGTAGAAGCAGACCTTGAAGTAGTTCAAGGAAAGCCCCTCCCTACACTATGGAATACGATCAAAAGCTTTCTCTCTGTGACGAAACGGATATTTGGGTGCAAGTCTCAAGCACACAAACTAGAAATTCTAAAAGATGTCAGTGGCATCATCAAGCCTTCAAGGATGACTCTATTACTTGGTCCTCCAGGCAGTGGGAAAACCACCTTATTACAGGCACTTGCTGGAAAACTTGACCACTCTCTCAAGATGACTGTGAGGGAAGCACTGGATTTCTCATCTCGTTGCCAGGGCATTGGAAGCAGAGCTGATATTATGAAAGAAGTTGCTCGGAGGGAAAAGGAAGCTGGTATTGTCCCAGAACCAGACATTGACACTTACATGAAGGCCATTTCAATTGAGGGGCTGAAAAAATCTCTAATGACAGACTATATAATAAAGATCCTTGGGCTGGACAATTGTGCTGATACAATTGTAGGGGATGCAATGCAAAGAGGAATTTCAGGTGGTCAAAAGAAAAGGCTAACAACAGGTGAAATGATGATTGGCCCAGCAAGAGCTTTGTTCATGGATGAGATATCAACAGGACTAGACAGCTCCACTACATTTCAGATTGTAACTTGTCTGCAGCAATTGACTCACGTAACAGAATCCACCATTTTGGTTTCACTTCTTCAGCCAACGCCAGAGACCTTTCATCTTTTTGATGACCTTATCTTGATGGCAAAAGGAAAAGTTGTATACCATGGGCCTAGTGATAATGTGGTAGAGTTCTTTGAGAACTGTGGTTTCCGTAGCCCACCACGAAAAGGCATTTCTGACTTCCTCCAAGAGGTGGTCTCTCCAAAGGATCAAGCAAAGTACTGGTACCATAAAGACCAACCCCACAGTTATGTTAGTGTGGATGAGTTCGTAAGTATGTTTAAGGACTTCCATGTTGGAAAAAAGCTTGAAGAGGAGCTTTCTAAGCCTTTCGAGCAATCAGAGTGCCACAAAAATGCTTTGTCATACAACCCTTACTCACTGAAAAAATGGGAACTATTCAAAGCATGCCTTTCTAGAGAATGGCTTCTGATGAAGCGAAACTCATTTGTTCATGTATTCGAATCAGCACAGCTTGTGTTCATTGCACTAGTAACAATGACAATGTTTCTACGTACATGGATGAAAGTCGACGAGATCTTTGCAAAGTACTACATGAGTGCTTTGTTTTATGCTCTAATTAGATTTCTATGCAATGCAAGTTCAGAGATATCAATGACTTCCTCAAGACTTGCAGTCTTCTACAAGCAGAGAGATTTCTACTTCTACCCTGCATGGGCTTATTCTATTCCGGCTGCCATCCTGAAGATTCCGTTTTCATTGCTAGATGCATTTCTTTGGACAGCCCTTACTTATTATGTGATTGGTTACAGTCCTGAACCTGAAAGGTTCTTCAGGCAATTTATTCTTCTGTTCCTCGTGCATCAAGTAGCAATATCACTATTTCGTTTGATCGCATCCTTGGTCAGAAATCCTTCTGTTGCAGGATCTGTTGGTCTTTTCTCTTTAATAGTAATGTTTCTATTCGGAGGCTTCATAATTCCAAAATCTTCTCTACCTGCTTGGTTGGCATGGGGATTTTGGGTTTCTCCATTGGCCTTTGCAGAAATAGGTGCTTCAATAAATGAATTTCATGCTCCAAGGTGGCAAAAGGTTTTATCTTCCAATGTTACTATAGGCCAGCAAGCTCTAATTAACCGTGGTCTAAACTTCAGTGATTACTTCTATTGGATATCCATAGGAGCTTTACTTGGATTTTGGATGGTTTTCAACCTTGGGTTCACATGTGCACTGAGTTATTCAAAGTCTCCAGGTAGTTCTCGGACTATCATTTCTCATGAAAGATACTCCCGTCTGAAGAAAAAAGAAATTTTGGATCATTCCTGTCAAGAAAGAGAATTAACAGTACTACAAATTGTAGATACGGACATAACTACTTCAGAATCTACAAATGCAGGCATGGTGTTGTCTTTTGAGCCCATAACTATATCATTTGAGAAACTGCAATACTTCATTGATACACCCCAGAAACTGCGAGACCAAGGTTTACCACCAAATCGACTACAGCTTCTTCAGAACATAACCGGAGCATTTAGACCTGGCGTTCTTACAGCACTGATGGGCGTTAGTGGAGCGGGAAAGACCACGCTGATGGATGTTCTTTCAGGCAGGAAAACAGGAGGAATTATCGAAGGCGACATCAGAATTGGAGGACACCCAAAAGTGCAACAAACATATGCCAGAATATCTGCGTATTGCGAGCAAACTGATATTCATTCTCCGGAGCTGACGGTAGAAGAGTCTATAGCATACTCAGCTTGGTTACGCTTGCCAGCCCAGATTGCCAGAAATGCTAGAGCTGAGTTTGTGAAGGAAGTACTTCAAATGATAGAGCTTGATGAAATCAAAGACGAGTTAGTTGGCATACCAGGTGGTAGTGGTATATCGACTGAACAGCGTAAAAGGCTAACAATTGCAGTAGAGCTTGTTTCAAATCCAACCATAATATTCATGGACGAACCCACATCCGGTTTAGATGCCCGAGCAGCTGCAATAGTTATGCGAGTTGTGAAAAATATCGTCAGTACAGGGAGGACTGTTGTATGCACTATTCATCAGCCAAGTATTGACATATTTGAAGCATTCGATGAGCTTCTTTTGATGAAAAGAGGAGGACAAGTAATTTACTGTGGAGAACTGGGTCAAGATTCAAGTAAGCTTATCCAATACTTTGAGGGGATTTCTGGGGTTCCAAAAATCAAAGATAATTACAACCCAGCAACATGGATGCTAGAAGTCACAAGTCCTTCTGAAGAAGCCAAACTTGCCTTAGATTTTGCTGAGCTTTATAGAGAGTCACATCTATGCCAGAAGAACAATGACCTAGTAAGAGAACACAGCTTTCCAGCACAAGGCTCAAAGGGATTGGATTTTCCTACTCGTTTCCCACGAAATGGGTGGGAGCAATTTAAAGCATGTCTGTGGAAGCGACATCTCTCCTACTGGAGAAATCCAAAATATAACCTCGGGCGGTTGTTAATCACTGCTGCATCCTCTATGATTTTTGGAGCACTTCTTTGGCAGAAAGGACAGAAAATAAATGGTGAACAGGAGTTCTTCAGCATTCTAGGATCAATATTTATTTTACAACAAAACATGGGAATTGGTAACTGTTCCTCTGTTTTACCATTTGTTGCTTCTGAGCGCAATGTTGTATACCGAGAAAGGTTTGCTGGAATGTACTCCTCATGGGCATATTCTTTATCACAGGTGGTCATTGAACTTCCCTATGTATTCCTTCAAGCAGTTTTGTTTTCGACCATTACATATCCAACCATTGGTTTCGACTGGTCACTTTACAAAGTATTCTGGTATGTGTATGCTATGTTTTGCACGCTGCTCTACTACACTTACTTCGGTATGTTGGTTGCTGCTGTGACCCCAACTTTCCAAGTAGCTTCAGTATTAGCAAGTTGCTGCTACACCTTGTTGAATCTGTTCTCCGGATACCTAATACCTCAACCAAACATTCCTAAATGGTGGGTTTGGGGCTACTGGATTTGCCCTTTATCGTGGTCCCTAAAAGGTACTCTCACTTCACAGTATGGGGATTTAAACGAAGAAATAGTACTCGGCAGGGAGGAAAAGACTATAAGTGCCTTCCTAGGAAGCATTTATGGATATAACTACGACGATCTAGGAATTGTAGGAATTGTTCTTCTTGCATATCCACTAGTTTTCGCTTTTGCGTTCGCAATTGCGACAGAAAAACTGAACTTTCAGAGGAGAGAAACCCTAAGGCCGCAGCCGGCCTTCCTTGGCGGCAGCGGCGCTGCAAATCTCTTGACTCTCTTTAGAGAGGTTTTCCGGCTACGCTGTGGCTTGGTTATTTGGAGTTGGAAGGGGGGCAGCACTTCTCTTTCTTCTCCCCTACCTGAAGGCAACGGAGATTGGCTTCGTCGAGGTCCCAAGATGCTGGAGTTGGCTCGGGTTTGTCCTCTTGAGGGACCGAAGCCTCTGAGGGTAGTCAGATTGGCTGCGTTAACGGGCTTCTGGGATGTGATGGATTGCTGGGATCGAGTCGGCGCCGTCGGCGTGGTGCATTGTCCAGGTGGTTGTTTTGGACCAGGATTGGCTTTAGGCCGGGGAATGGCTACGGACACTGCTGTGTTTGTTCGTTTTCATGGTTTGGAGGATGAAGATGATGGCGCAGCGGCCCTGACGGCTTCGATAGTTCTTCGTCCTAGGGACCCTTTGTCGGCCTTCGTGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1503

Amino Acids

168.73

Weight (kDa)

8.5

Isoelectric Point (pI)

38.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 108 - 256 5.8e-12 ABC transporter
ABC2_membrane_7 PF19055 290 - 345 9.4e-07 ABC-2 type transporter
ABC2_membrane PF01061 411 - 622 3.4e-41 ABC-2 type transporter
PDR_assoc PF08370 627 - 689 1.9e-20 Plant PDR ABC transporter associated
ABC_tran PF00005 769 - 921 5.6e-20 ABC transporter
ABC2_membrane PF01061 1066 - 1279 5.2e-56 ABC-2 type transporter
ABC2_membrane_3 PF12698 1115 - 1314 3e-06 ABC-2 family transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000472)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15230 AT4G15230 AT4G15230 AT4G15230 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15236
fragaria_vesca FvH4_4g21360 FvH4_4g21360 FvH4_4g21390 FvH4_6g49431 FvH4_6g49450
malus_domestica MD09G1044300.v1.1 MD16G1137900.v1.1 MD16G1138000.v1.1
prunus_persica Prupe.1G106800_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.3G273300_v2.0.a1
pyrus_communis pycom111g03460 pycom16g12210
rosa_chinensis RchiOBHm_Chr2g0169331 RchiOBHm_Chr2g0169361 RchiOBHm_Chr2g0169371 RchiOBHm_Chr2g0169381 RchiOBHm_Chr2g0169411 RchiOBHm_Chr4g0427141 RchiOBHm_Chr4g0427321
rosa_laevigata RLG00000007252 RLG00000007258 RLG00000013974 RLG00000021856 RLG00000021857 RLG00000021858 RLG00000021859
rosa_multiflora Rmu_sc0000648.1_g000007 Rmu_sc0000648.1_g000009 Rmu_sc0003813.1_g000016 Rmu_sc0009165.1_g000008 Rmu_sc0022554.1_g000001
rosa_roxburghii Rroxscaffold_2G00082200 Rroxscaffold_2G00082230 Rroxscaffold_5G00368960 Rroxscaffold_5G00369010 Rroxscaffold_7G00200390
rosa_rugosa Rorug02G0539500 Rorug02G0539600 Rorug02G0539700 Rorug04G0216400 Rorug04G0216500 Rorug04G0216500 Rorug04G0216600
rosa_samantha Rh2AG610800 Rh2AG611000 Rh2AG611100 Rh2CG592800 Rh2CG593000 Rh2CG593200 Rh2DG634900 Rh2DG635100 Rh2DG635200 Rh2DG635400 Rh4AG272200 Rh4AG272900 Rh4DG275600 Rh4DG276000
rosa_wichuraiana Rw2G050640 Rw2G050660 Rw2G050670 Rw2G050680 Rw4G023610 Rw4G023680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1801
Acc65I GGTACC 1 cut(s) 1059
AccB1I GGYRCC 2 cut(s) 1059, 4319
AccB7I CCANNNNNTGG 4 cut(s) 386, 1027, 3188, 3572
AccBSI CCGCTC 1 cut(s) 2369
AccI GTMKAC 3 cut(s) 933, 1365, 3450
AccIII TCCGGA 2 cut(s) 2515, 3737
AciI CCGC 6 cut(s) 2369, 3265, 4037, 4056, 4062, 4448
AclWI GGATC 6 cut(s) 628, 1050, 1729, 2118, 3373, 4317
AcsI RAATTY 4 cut(s) 318, 690, 1862, 2103
AcyI GRCGYC 1 cut(s) 4320
AflIII ACRYGT 1 cut(s) 2018
AhdI GACNNNNNGTC 2 cut(s) 1469, 4489
AhlI ACTAGT 2 cut(s) 1324, 3967
AjnI CCWGG 7 cut(s) 379, 480, 2044, 2332, 2662, 4342, 4360
AjuI GAANNNNNNNTTGG 2 cut(s) 720, 752
AleI CACNNNNGTG 1 cut(s) 4400
Alw21I GWGCWC 4 cut(s) 35, 55, 2026, 3307
Alw26I GTCTC 5 cut(s) 303, 875, 1036, 2046, 2265
Alw44I GTGCAC 1 cut(s) 2022
AlwI GGATC 6 cut(s) 628, 1050, 1729, 2118, 3373, 4317
AlwNI CAGNNNCTG 2 cut(s) 1724, 2266
Ama87I CYCGRG 3 cut(s) 2775, 3260, 4221
Aor13HI TCCGGA 2 cut(s) 2515, 3737
ApaLI GTGCAC 1 cut(s) 2022
ApoI RAATTY 4 cut(s) 318, 690, 1862, 2103
ArsI GACNNNNNNTTYG 2 cut(s) 1358, 1390
Asp700I GAANNNNTTC 5 cut(s) 216, 1260, 1708, 2613, 3731
Asp718I GGTACC 1 cut(s) 1059
AspA2I CCTAGG 2 cut(s) 3898, 4478
AspLEI GCGC 4 cut(s) 3441, 4067, 4322, 4445
AspS9I GGNCC 9 cut(s) 374, 740, 941, 3810, 4199, 4241, 4358, 4450, 4483
AsuC2I CCSGG 1 cut(s) 4379
AsuHPI GGTGA 2 cut(s) 737, 3350
AsuII TTCGAA 1 cut(s) 1296
AvaI CYCGRG 3 cut(s) 2775, 3260, 4221
AvaII GGWCC 6 cut(s) 374, 3810, 4199, 4241, 4358, 4483
AvrII CCTAGG 2 cut(s) 3898, 4478
AxyI CCTNAGG 1 cut(s) 4031
BaeGI GKGCMC 1 cut(s) 2026
BaeI ACNNNNGTAYC 4 cut(s) 651, 684, 2071, 2104
BanI GGYRCC 2 cut(s) 1059, 4319
BanII GRGCYC 2 cut(s) 55, 2214
BarI GAAGNNNNNNTAC 4 cut(s) 3814, 3846, 3846, 3878
BauI CACGAG 1 cut(s) 1652
BbsI GAAGAC 1 cut(s) 1465
Bbv12I GWGCWC 4 cut(s) 35, 55, 2026, 3307
BccI CCATC 7 cut(s) 907, 1541, 1990, 2347, 2380, 4291, 4433
BceAI ACGGC 3 cut(s) 77, 4307, 4473
BcgI CGANNNNNNTGC 2 cut(s) 1358, 1392
BciT130I CCWGG 7 cut(s) 381, 482, 2046, 2334, 2664, 4344, 4362
BciVI GTATCC 1 cut(s) 3734
BcnI CCSGG 1 cut(s) 4379
BcoDI GTCTC 5 cut(s) 303, 875, 1036, 2046, 2265
BcuI ACTAGT 2 cut(s) 1324, 3967
BfmI CTRYAG 5 cut(s) 9, 818, 1908, 2297, 2538
BfoI RGCGCY 2 cut(s) 4068, 4323
BfuAI ACCTGC 1 cut(s) 1801
BfuI GTATCC 1 cut(s) 3734
BglI GCCNNNNNGGC 1 cut(s) 4457
BglII AGATCT 1 cut(s) 1371
BlnI CCTAGG 2 cut(s) 3898, 4478
BlpI GCTNAGC 1 cut(s) 3075
BmcAI AGTACT 5 cut(s) 1055, 1385, 2142, 2613, 3864
Bme1390I CCNGG 8 cut(s) 381, 482, 2046, 2334, 2664, 4344, 4362, 4379
Bme18I GGWCC 6 cut(s) 374, 3810, 4199, 4241, 4358, 4483
BmeRI GACNNNNNGTC 2 cut(s) 1469, 4489
BmeT110I CYCGRG 3 cut(s) 2775, 3260, 4221
BmgT120I GGNCC 9 cut(s) 374, 740, 941, 3810, 4199, 4241, 4358, 4450, 4483
BmiI GGNNCC 8 cut(s) 1061, 2985, 3812, 4201, 4242, 4321, 4484, 4485
BmrFI CCNGG 8 cut(s) 381, 482, 2046, 2334, 2664, 4344, 4362, 4379
BmrI ACTGGG 1 cut(s) 2942
BmsI GCATC 9 cut(s) 348, 664, 1552, 1666, 1700, 2761, 3012, 3293, 4197
BmuI ACTGGG 1 cut(s) 2942
BpiI GAAGAC 1 cut(s) 1465
BplI GAGNNNNNCTC 2 cut(s) 1016, 1048
BpmI CTGGAG 4 cut(s) 363, 2028, 3259, 4232
Bpu10I CCTNAGC 1 cut(s) 54
Bpu1102I GCTNAGC 1 cut(s) 3075
Bpu14I TTCGAA 1 cut(s) 1296
BpuEI CTTGAG 4 cut(s) 285, 422, 1444, 4256
BpuMI CCSGG 1 cut(s) 4379
BsaAI YACGTR 2 cut(s) 835, 1349
BsaHI GRCGYC 1 cut(s) 4320
BsaI GGTCTC 3 cut(s) 875, 1036, 2265
BsaWI WCCGGW 4 cut(s) 2318, 2515, 2762, 3737
BsaXI ACNNNNNCTCC 2 cut(s) 2898, 2928
Bse118I RCCGGY 1 cut(s) 4042
Bse1I ACTGG 6 cut(s) 465, 1061, 2937, 3242, 3587, 3794
Bse21I CCTNAGG 1 cut(s) 4031
Bse3DI GCAATG 5 cut(s) 684, 1317, 1435, 1553, 3448
BseAI TCCGGA 2 cut(s) 2515, 3737
BseBI CCWGG 7 cut(s) 381, 482, 2046, 2334, 2664, 4344, 4362
BseMI GCAATG 5 cut(s) 684, 1317, 1435, 1553, 3448
BseMII CTCAG 7 cut(s) 68, 2018, 2562, 2586, 3066, 3426, 4244
BseNI ACTGG 6 cut(s) 465, 1061, 2937, 3242, 3587, 3794
BseRI GAGGAG 5 cut(s) 114, 1163, 2919, 3469, 4033
BseSI GKGCMC 1 cut(s) 2026
BseYI CCCAGC 3 cut(s) 742, 3010, 4305
BshNI GGYRCC 2 cut(s) 1059, 4319
BsiHKAI GWGCWC 4 cut(s) 35, 55, 2026, 3307
BsiHKCI CYCGRG 3 cut(s) 2775, 3260, 4221
BsiSI CCGG 8 cut(s) 1526, 2319, 2516, 2763, 3738, 4043, 4102, 4378
BslFI GGGAC 6 cut(s) 165, 533, 3796, 4185, 4254, 4496
BsmAI GTCTC 5 cut(s) 303, 875, 1036, 2046, 2265
BsmFI GGGAC 6 cut(s) 165, 533, 3796, 4185, 4254, 4496
BsmI GAATGC 2 cut(s) 2876, 3357
Bso31I GGTCTC 3 cut(s) 875, 1036, 2265
BsoBI CYCGRG 3 cut(s) 2775, 3260, 4221
Bsp119I TTCGAA 1 cut(s) 1296
Bsp1286I GDGCHC 5 cut(s) 35, 55, 2026, 2214, 3307
Bsp13I TCCGGA 2 cut(s) 2515, 3737
Bsp1720I GCTNAGC 1 cut(s) 3075
Bsp19I CCATGG 1 cut(s) 937
BspACI CCGC 6 cut(s) 2369, 3265, 4037, 4056, 4062, 4448
BspCNI CTCAG 7 cut(s) 67, 2019, 2561, 2587, 3067, 3427, 4245
BspEI TCCGGA 2 cut(s) 2515, 3737
BspHI TCATGA 1 cut(s) 2071
BspLI GGNNCC 8 cut(s) 1061, 2985, 3812, 4201, 4242, 4321, 4484, 4485
BspMAI CTGCAG 1 cut(s) 822
BspMI ACCTGC 1 cut(s) 1801
BspPI GGATC 6 cut(s) 628, 1050, 1729, 2118, 3373, 4317
BspT104I TTCGAA 1 cut(s) 1296
BspT107I GGYRCC 2 cut(s) 1059, 4319
BspTNI GGTCTC 3 cut(s) 875, 1036, 2265
BsrBI CCGCTC 1 cut(s) 2369
BsrDI GCAATG 5 cut(s) 684, 1317, 1435, 1553, 3448
BsrFI RCCGGY 1 cut(s) 4042
BsrI ACTGG 6 cut(s) 465, 1061, 2937, 3242, 3587, 3794
BssAI RCCGGY 1 cut(s) 4042
BssNAI GTATAC 2 cut(s) 934, 3451
BssNI GRCGYC 1 cut(s) 4320
BssSI CACGAG 1 cut(s) 1652
BssT1I CCWWGG 9 cut(s) 637, 937, 1695, 1874, 2008, 2275, 3898, 4051, 4478
Bst1107I GTATAC 2 cut(s) 934, 3451
Bst2BI CACGAG 1 cut(s) 1652
Bst2UI CCWGG 7 cut(s) 381, 482, 2046, 2334, 2664, 4344, 4362
Bst6I CTCTTC 2 cut(s) 1140, 2526
BstACI GRCGYC 1 cut(s) 4320
BstBAI YACGTR 2 cut(s) 835, 1349
BstBI TTCGAA 1 cut(s) 1296
BstC8I GCNNGC 9 cut(s) 1243, 1916, 1920, 2191, 2495, 2564, 2568, 3629, 4044
BstDSI CCRYGG 2 cut(s) 937, 1933
BstENI CCTNNNNNAGG 1 cut(s) 1022
BstH2I RGCGCY 2 cut(s) 4068, 4323
BstHHI GCGC 4 cut(s) 3441, 4067, 4322, 4445
BstMAI GTCTC 5 cut(s) 303, 875, 1036, 2046, 2265
BstNI CCWGG 7 cut(s) 381, 482, 2046, 2334, 2664, 4344, 4362
BstNSI RCATGY 3 cut(s) 1245, 2022, 3213
BstSCI CCNGG 8 cut(s) 379, 480, 2044, 2332, 2662, 4342, 4360, 4377
BstSFI CTRYAG 5 cut(s) 9, 818, 1908, 2297, 2538
BstSLI GKGCMC 1 cut(s) 2026
BstSNI TACGTA 1 cut(s) 1349
BstV2I GAAGAC 1 cut(s) 1465
BstX2I RGATCY 3 cut(s) 633, 1371, 1721
BstXI CCANNNNNNTGG 5 cut(s) 488, 853, 2386, 3018, 4210
BstYI RGATCY 3 cut(s) 633, 1371, 1721
BstZ17I GTATAC 2 cut(s) 934, 3451
Bsu36I CCTNAGG 1 cut(s) 4031
BsuI GTATCC 1 cut(s) 3734
BtgI CCRYGG 2 cut(s) 937, 1933
BtsI GCAGTG 3 cut(s) 391, 3276, 4395
BtsIMutI CAGTG 9 cut(s) 340, 391, 458, 1214, 1954, 2024, 2348, 3276, 4395
BveI ACCTGC 1 cut(s) 1801
Cac8I GCNNGC 9 cut(s) 1243, 1916, 1920, 2191, 2495, 2564, 2568, 3629, 4044
CaiI CAGNNNCTG 2 cut(s) 1724, 2266
CciI TCATGA 1 cut(s) 2071
CfoI GCGC 4 cut(s) 3441, 4067, 4322, 4445
Cfr10I RCCGGY 1 cut(s) 4042
Cfr13I GGNCC 9 cut(s) 374, 740, 941, 3810, 4199, 4241, 4358, 4450, 4483
CsiI ACCWGGT 1 cut(s) 2662
DinI GGCGCC 1 cut(s) 4321
DraI TTTAAA 2 cut(s) 3205, 3849
DriI GACNNNNNGTC 2 cut(s) 1469, 4489
Eam1104I CTCTTC 2 cut(s) 1140, 2526
Eam1105I GACNNNNNGTC 2 cut(s) 1469, 4489
EarI CTCTTC 2 cut(s) 1140, 2526
Ecl136II GAGCTC 1 cut(s) 53
Eco105I TACGTA 1 cut(s) 1349
Eco130I CCWWGG 9 cut(s) 637, 937, 1695, 1874, 2008, 2275, 3898, 4051, 4478
Eco24I GRGCYC 2 cut(s) 55, 2214
Eco31I GGTCTC 3 cut(s) 875, 1036, 2265
Eco32I GATATC 3 cut(s) 771, 1446, 1968
Eco47I GGWCC 6 cut(s) 374, 3810, 4199, 4241, 4358, 4483
Eco53kI GAGCTC 1 cut(s) 53
Eco81I CCTNAGG 1 cut(s) 4031
Eco88I CYCGRG 3 cut(s) 2775, 3260, 4221
EcoICRI GAGCTC 1 cut(s) 53
EcoNI CCTNNNNNAGG 1 cut(s) 1022
EcoO109I RGGNCCY 2 cut(s) 4199, 4483
EcoRII CCWGG 7 cut(s) 379, 480, 2044, 2332, 2662, 4342, 4360
EcoRV GATATC 3 cut(s) 771, 1446, 1968
EcoT14I CCWWGG 9 cut(s) 637, 937, 1695, 1874, 2008, 2275, 3898, 4051, 4478
EcoT22I ATGCAT 1 cut(s) 1567
EcoT38I GRGCYC 2 cut(s) 55, 2214
EgeI GGCGCC 1 cut(s) 4321
EheI GGCGCC 1 cut(s) 4321
ErhI CCWWGG 9 cut(s) 637, 937, 1695, 1874, 2008, 2275, 3898, 4051, 4478
FalI AAGNNNNNCTT 8 cut(s) 192, 224, 1137, 1169, 1230, 1262, 3042, 3074
FaqI GGGAC 6 cut(s) 165, 533, 3796, 4185, 4254, 4496
FauI CCCGC 1 cut(s) 2362
FauNDI CATATG 1 cut(s) 2470
FblI GTMKAC 3 cut(s) 933, 1365, 3450
FriOI GRGCYC 2 cut(s) 55, 2214
GlaI GCGC 4 cut(s) 3440, 4066, 4321, 4444
GsaI CCCAGC 3 cut(s) 746, 3014, 4309
GsuI CTGGAG 4 cut(s) 363, 2028, 3259, 4232
HaeII RGCGCY 2 cut(s) 4068, 4323
HapII CCGG 8 cut(s) 1526, 2319, 2516, 2763, 3738, 4043, 4102, 4378
HhaI GCGC 4 cut(s) 3441, 4067, 4322, 4445
Hin1I GRCGYC 1 cut(s) 4320
Hin6I GCGC 4 cut(s) 3439, 4065, 4320, 4443
HinP1I GCGC 4 cut(s) 3439, 4065, 4320, 4443
HincII GTYRAC 2 cut(s) 1366, 4278
HindII GTYRAC 2 cut(s) 1366, 4278
HindIII AAGCTT 3 cut(s) 259, 1139, 2951
HpaI GTTAAC 1 cut(s) 4278
HpaII CCGG 8 cut(s) 1526, 2319, 2516, 2763, 3738, 4043, 4102, 4378
HphI GGTGA 2 cut(s) 737, 3350
Hpy166II GTNNAC 8 cut(s) 934, 1366, 2016, 2024, 2282, 3341, 3451, 4278
Hpy8I GTNNAC 8 cut(s) 934, 1366, 2016, 2024, 2282, 3341, 3451, 4278
Hpy99I CGWCG 4 cut(s) 1370, 3929, 4197, 4328
HpyCH4IV ACGT 2 cut(s) 834, 1348
HpySE526I ACGT 2 cut(s) 834, 1348
Hsp92I GRCGYC 1 cut(s) 4320
HspAI GCGC 4 cut(s) 3439, 4065, 4320, 4443
KasI GGCGCC 1 cut(s) 4319
KflI GGGWCCC 1 cut(s) 4483
Kpn2I TCCGGA 2 cut(s) 2515, 3737
KpnI GGTACC 1 cut(s) 1063
KroI GCCGGC 1 cut(s) 4042
KroNI GCCGGC 1 cut(s) 4044
KspAI GTTAAC 1 cut(s) 4278
LweI GCATC 9 cut(s) 348, 664, 1552, 1666, 1700, 2761, 3012, 3293, 4197
MabI ACCWGGT 1 cut(s) 2662
MaeII ACGT 2 cut(s) 834, 1348
MbiI CCGCTC 1 cut(s) 2369
MfeI CAATTG 6 cut(s) 588, 649, 663, 824, 2706, 3990
MflI RGATCY 3 cut(s) 633, 1371, 1721
MhlI GDGCHC 5 cut(s) 35, 55, 2026, 2214, 3307
Mly113I GGCGCC 1 cut(s) 4320
MlyI GAGTC 6 cut(s) 355, 823, 2543, 3098, 4075, 4323
MmeI TCCRAC 4 cut(s) 1111, 2756, 3590, 4112
Mph1103I ATGCAT 1 cut(s) 1567
MroI TCCGGA 2 cut(s) 2515, 3737
MroNI GCCGGC 1 cut(s) 4042
MroXI GAANNNNTTC 5 cut(s) 216, 1260, 1708, 2613, 3731
MslI CAYNNNNRTG 7 cut(s) 572, 1083, 1188, 2384, 3097, 3214, 4400
MspA1I CMGCKG 3 cut(s) 2783, 4062, 4448
MspI CCGG 8 cut(s) 1526, 2319, 2516, 2763, 3738, 4043, 4102, 4378
MspR9I CCNGG 8 cut(s) 381, 482, 2046, 2334, 2664, 4344, 4362, 4379
MunI CAATTG 6 cut(s) 588, 649, 663, 824, 2706, 3990
Mva1269I GAATGC 2 cut(s) 2876, 3357
MvaI CCWGG 7 cut(s) 381, 482, 2046, 2334, 2664, 4344, 4362
NaeI GCCGGC 1 cut(s) 4044
NarI GGCGCC 1 cut(s) 4320
NciI CCSGG 1 cut(s) 4379
NcoI CCATGG 1 cut(s) 937
NdeI CATATG 1 cut(s) 2470
NgoMIV GCCGGC 1 cut(s) 4042
NlaIV GGNNCC 8 cut(s) 1061, 2985, 3812, 4201, 4242, 4321, 4484, 4485
NmeAIII GCCGAG 1 cut(s) 3846
NmuCI GTSAC 5 cut(s) 271, 3031, 3090, 3585, 3670
NsiI ATGCAT 1 cut(s) 1567
NspI RCATGY 3 cut(s) 1245, 2022, 3213
NspV TTCGAA 1 cut(s) 1296
OliI CACNNNNGTG 1 cut(s) 4400
PaeI GCATGC 1 cut(s) 1245
PagI TCATGA 1 cut(s) 2071
PciI ACATGT 1 cut(s) 2018
PcsI WCGNNNNNNNCGW 1 cut(s) 3181
PctI GAATGC 2 cut(s) 2876, 3357
PdiI GCCGGC 1 cut(s) 4044
PdmI GAANNNNTTC 5 cut(s) 216, 1260, 1708, 2613, 3731
PfeI GAWTC 6 cut(s) 842, 1298, 1543, 2177, 2942, 3727
PflMI CCANNNNNTGG 4 cut(s) 386, 1027, 3188, 3572
PleI GAGTC 6 cut(s) 355, 823, 2542, 3097, 4075, 4322
PluTI GGCGCC 1 cut(s) 4323
PpsI GAGTC 6 cut(s) 355, 823, 2542, 3097, 4075, 4322
Ppu21I YACGTR 2 cut(s) 835, 1349
PpuMI RGGWCCY 2 cut(s) 4199, 4483
PscI ACATGT 1 cut(s) 2018
Psp124BI GAGCTC 1 cut(s) 55
Psp5II RGGWCCY 2 cut(s) 4199, 4483
Psp6I CCWGG 7 cut(s) 379, 480, 2044, 2332, 2662, 4342, 4360
PspFI CCCAGC 3 cut(s) 742, 3010, 4305
PspGI CCWGG 7 cut(s) 379, 480, 2044, 2332, 2662, 4342, 4360
PspN4I GGNNCC 8 cut(s) 1061, 2985, 3812, 4201, 4242, 4321, 4484, 4485
PspPI GGNCC 9 cut(s) 374, 740, 941, 3810, 4199, 4241, 4358, 4450, 4483
PspPPI RGGWCCY 2 cut(s) 4199, 4483
PsrI GAACNNNNNNTAC 2 cut(s) 3933, 3965
PstI CTGCAG 1 cut(s) 822
PstNI CAGNNNCTG 2 cut(s) 1724, 2266
PsuI RGATCY 3 cut(s) 633, 1371, 1721
PvuII CAGCTG 1 cut(s) 2783
RseI CAYNNNNRTG 7 cut(s) 572, 1083, 1188, 2384, 3097, 3214, 4400
SacI GAGCTC 1 cut(s) 55
SalI GTCGAC 1 cut(s) 1364
Sau96I GGNCC 9 cut(s) 374, 740, 941, 3810, 4199, 4241, 4358, 4450, 4483
ScaI AGTACT 5 cut(s) 1055, 1385, 2142, 2613, 3864
SchI GAGTC 6 cut(s) 355, 823, 2543, 3098, 4075, 4323
ScrFI CCNGG 8 cut(s) 381, 482, 2046, 2334, 2664, 4344, 4362, 4379
SduI GDGCHC 5 cut(s) 35, 55, 2026, 2214, 3307
SexAI ACCWGGT 1 cut(s) 2662
SfaNI GCATC 9 cut(s) 348, 664, 1552, 1666, 1700, 2761, 3012, 3293, 4197
SfcI CTRYAG 5 cut(s) 9, 818, 1908, 2297, 2538
SfoI GGCGCC 1 cut(s) 4321
SfuI TTCGAA 1 cut(s) 1296
SinI GGWCC 6 cut(s) 374, 3810, 4199, 4241, 4358, 4483
SmiMI CAYNNNNRTG 7 cut(s) 572, 1083, 1188, 2384, 3097, 3214, 4400
SmlI CTYRAG 4 cut(s) 300, 437, 1459, 4235
SmoI CTYRAG 4 cut(s) 300, 437, 1459, 4235
SnaBI TACGTA 1 cut(s) 1349
SpeI ACTAGT 2 cut(s) 1324, 3967
SphI GCATGC 1 cut(s) 1245
SsiI CCGC 6 cut(s) 2369, 3265, 4037, 4056, 4062, 4448
SspDI GGCGCC 1 cut(s) 4319
SspI AATATT 2 cut(s) 2742, 3372
SstI GAGCTC 1 cut(s) 55
StyD4I CCNGG 8 cut(s) 379, 480, 2044, 2332, 2662, 4342, 4360, 4377
StyI CCWWGG 9 cut(s) 637, 937, 1695, 1874, 2008, 2275, 3898, 4051, 4478
TaiI ACGT 2 cut(s) 837, 1351
TaqII GACCGA 1 cut(s) 4258
TatI WGTACW 7 cut(s) 1053, 1383, 2140, 2611, 2819, 3474, 3862
TauI GCSGC 4 cut(s) 4039, 4059, 4065, 4451
TfiI GAWTC 6 cut(s) 842, 1298, 1543, 2177, 2942, 3727
TscAI CASTG 9 cut(s) 340, 391, 465, 1221, 1954, 2031, 2355, 3283, 4402
TseFI GTSAC 5 cut(s) 271, 3031, 3090, 3585, 3670
Tsp45I GTSAC 5 cut(s) 271, 3031, 3090, 3585, 3670
TspGWI ACGGA 6 cut(s) 295, 974, 1536, 2174, 4194, 4406
TspRI CASTG 9 cut(s) 340, 391, 465, 1221, 1954, 2031, 2355, 3283, 4402
Van91I CCANNNNNTGG 4 cut(s) 386, 1027, 3188, 3572
VneI GTGCAC 1 cut(s) 2022
VpaK11BI GGWCC 6 cut(s) 374, 3810, 4199, 4241, 4358, 4483
XagI CCTNNNNNAGG 1 cut(s) 1022
XapI RAATTY 4 cut(s) 318, 690, 1862, 2103
XbaI TCTAGA 1 cut(s) 1250
XceI RCATGY 3 cut(s) 1245, 2022, 3213
XcmI CCANNNNNNNNNTGG 2 cut(s) 2743, 3189
XmaJI CCTAGG 2 cut(s) 3898, 4478
XmiI GTMKAC 3 cut(s) 933, 1365, 3450
XmnI GAANNNNTTC 5 cut(s) 216, 1260, 1708, 2613, 3731
ZrmI AGTACT 5 cut(s) 1055, 1385, 2142, 2613, 3864
Zsp2I ATGCAT 1 cut(s) 1567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.