Rw2G050660

pleiotropic drug resistance protein 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000472
Physical Location & Seq
Forward (+)
78283857 .. 78294829
10973 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G050660.1

Sequence Viewer

Length: 4311 bp
ATGGATTTGGCTGCAGAGATAGGAAGAAGTGTGAGGACTTCTTTTCATCAGCATGCATCAAGTTTCAGGAGCACTTCAGATGTCAGGTCCATGAAAGAGAATGATGACGATGAAGTTGAGTTGCGGTGGGCTGCCATTGAAAGATTGCCCACATTTGAACGTATTCGAACTTCACTCTTCGGTTGTAACAAGGAGGACGATGATCATGAAGGTAAGAGTAAGAGGGTAGTTGATGTTACAAAGCTAGAAGCCCTGGAACGTCGTGTGTTCATTGATAAACTTCTCAAGCAAATAGAAGGAGATAATCAATGCCTCTTACAGAAACTCAAAGAAAGAATAGACAGTGGGACTGCAAATGCCGACTGTGGAAGTACGATACAAAAATTTGTTGTAGAAGCAGAATGTGAAGTAGTTCAAGGAAAGCCCCTCCCAACACTATGGAACACGCTCAAAAGCTTTCTCTCTGTCATGACACAGATAATTGGATGCAAGTCTGAAGCATACAATCTAGAAATTCTCAAAGATGTCAGTGGCATCATCAAGCCTTCAAGGATGACTCTATTGCTTGGTCCTCCAGGCTGTGGAAAAACTACCTTATTACAGGCACTTGCAGGAAAACTTAACCACCCTCTCAAGGTTCAAGGAGAAATAACCTACAATGGTTATAAGCTCAATGAGTTTGTTCCTCAGAAGACATCAGCTTACATAAGTCAATATGACTTGCATATATCTGAGATGACTGTGAGGGAAACACTGGATTTCTCAGCTCGTTGCCAGGGCATTGGAAGCAGAGCTGATATTATGAAAGAAGTTGCTAGAAGGGAGAAGCAAGCTGGTATTGTCCCAGAACCAGATATTGACACTTATATGAAGGCCATTTCAATTGAAGGGTTGAAAAAATCTCTAATGACAGACTATATAATAAAGATCCTTGGGCTGGACATTTGTGCTGAGACAATCACAGGGGACCCAATGCAAAGAGGAATTTCGGGTGGTCAAAAGAAAAGGCTAACAACAGGTGAGATGATGATTGGCTCAGCAAGAGCTTTCTTCATGGATGAGATATCAACAGGACTAGACAGCTCCACTACATTTCAGATTGTAACTTGTCTGCAGCAATTGACTCACGTAACAGAATCGTCCATTTTGGTTTCACTTCTTCAGCCAACGCCAGAGACCTTTCATCTGTTTGATGACATTATCTTGATGGCTGAAGGAAAAGTTGTATACCATGGGCCTAGTGACAATGTTGTGGAGTTCTTTCAGAAGTGTGGTTTCCTTAGCCCACCACGAAAAGGCATTTCTGACTTCCTCCAAGAGGTGGTTTCTCAAAAGGATCAAGCAAAGTACTGGTGCCATAAAGACCAACCCTACAGTTATGTTAGTGTTGATGAGTTCGTAAGTATGTTTAAGGACTTCCATGTCGGAAAGAAGCTTGAAGAGGAACTTTCCAAGCCTTTTGAGCAATCAGAGTGCCATAAGCATGCTTTGTCATACAACATTTACTCACTGAGAAAATGGGAACTATTCAAAGCATGCCTTTCTAGAGAATGGCTTCTCATGAAGCGAAACTCTTTTGTTCATGTATTCCAATCAGTACAGCTTGTGGTCATTGCACTAGTAACAATGACAATGTTTCTACGTACATGGATGAAAGTCAACGAGATCTATGCAAAGTACTACATGAGTGCTTTGTTTTATGCTCTAATTAGATTTCTATGCAATGCAAGTTCAGAGATATCAATGACTTCCTCTAGACTTGCAGTCTTCTATAAGCAAAGAGATGTCTACTTTTACCCTGCATGGGCTTATTCTATTCCGGCTGCCATCCTGAAGATTCCATTTTCATTGCTAGATGCATTTCTTTGGACAGCCCTTACTTATTATGTGATTGGTTACAGTCCTGAACCTGAAAGGTTCTTCAGGCAATTTATTCTTCTGTTCCTCGTGCATCAAGTAGCAATATCACTATTTCGTTTGATTGCATCCTTGGTCCGAAATCCTTCTGTTGCAGGATCTGTCGGCCTTTTATCTTTAATAGTAATGTTTCTATTTGGAGGCTTCATAATTCCAAAATCTTCTCTACCTGCATGGTTGGAATGGGGATTTTGGGTTTCTCCATTGGCCTTTGCAGAACTAGGTGTTTCAATAAATGAATTTCATGCTCCAAGGTGGCAAAAGGTTTTATCTTCCAATGTCACTATTGGCCAGCAAGCTCTAATTAATCGTGGTCTAAACTTCAATGATTACTTCTATTGGATATCCATAGGAGCTTTAATTGGATTTTGGATGGTTTTCAACCTGGGGTTCACATGGGCATTGAGTTATTCAAAGTCTCCAGGTAGTTCTCGGACTATCATTTCTCATGAAAAATTCTCCCGTCTGAAGAAAAAAGAAATTTTAGATAATTGCGGTCAAGAAAGAGAATTAGCTAGTGTAGATACTGATATAACTACTTCGGAATCTACAAATGCAGGCATGGTGTTGCCTTTTGAGCCCATTACTATATCATTTGAGAAACTGCAATACTTTATTGATACACCCCAGAAACTGAGAGAGCAAGGTTTCCCACCAAATCGACTACAGCTTCTTCAAGATATAACTGGTGCATTTAGACCTGGCGTTCTTACAGCACTGATGGGTGTTAGTGGAGCAGGAAAGACAACACTGATGGATGTTCTTTCTGGAAGGAAAACTGGTGGACTTATTGAAGGAGACATTAGAATTAGAGGACACCCAAAAGTCCAAGAAACATATGCCAGAATATCTGGGTATTGCGAGCAAAGTGATATTCATTCTCCAGAGCTTACAGTAGAAGAGTCAGTAGCATACTCAGCTTGGTTACGCTTGCCATCCCAGATTGCTAGAAATACTAGAGCTGAGTTTGTGAAAGAAGTACTTCATATGATAGAGCTTGATGAAATCAAAGATGAGTTAGTTGGCATACCAGGTGGTAGTGGTATATCGACTGAACAGCGTAAAAGGCTAACAATAGCAGTAGAGCTTGTTTCAAATCCATCCGTTATATTTATGGATGAACCCACATCTGGTTTAGATGCCAGAGCAGCTGCAATAGTTATGAGAGTTGTGAAAAACATTGTCAGTACAAGAAGGACTGTTGTTTGCACTATTCATCAGCCAAGTATTGACATATTTGAAGCATTCGATGAGCTTCTTTTGATGAAAAGAGGAGGACAAGTAATATATTGTGGAGAGCTGGGTCAAGATTCAAGTAAGCTTATCCAATACTTTGAGGGTATTTCTGGGGTTCCAAAAATCAAAGATAATTACAACCCAGCAACATGGATGTTAGAAGTCACAAGTCCTTCTGCAGAAGCCAAACTTGCCTTAGATTTTGCTCAGCTTTATAGAGAATCACATCTATGCCAGAAGAACAATGACCTAGTTAGAGAACACAGCTTACCAGCACAAGGCTCAAAGGGATTGGATTTTCCTACTCGTTTCCCACAAAATGGATGGGAGCAATTTAAAGCATGTCTGTGGAAGCGACATCTCTCCTACTGGAGAAATCCAAAATATAACCTGGGGCGATTGTTAATCACTGCTGCATCCTCTTTGATATTTGGAGCACTTCTTTGGCAGAAAGGACAGAAAATAAATGGTGAACAGGAGTTCTTCAGCATTCTAGGATCAATATTTATTTTACAACAAAACATGGGAATTGGTAATTGTTCCTCTGTTTTACCATTTGTCGCTTCTGAGCGCAATGTTGTATATCGAGAAAGGTTTGCTGGAATGTACTCCTCATGGGCATATTCTTTATCACAGGTGATCATTGAACTTCCTTATGTATTCTTTCAAGCAGTTTTGTTTTCGACCATTACATATCCATCGATTGGTTTCCACTGGTCACTTTACAAAGTATTCTGGTATGTGTATGCTATGTTTTTCACGCTGCTCTACTTCACTTACTTCGGTATATTGGTTGCTTCTGTGACCCCAACTTTCCAAGTAGCTTCAGTATTAGCAAGTTTCTGCTACACCATGCTCAATCTGTTCTCGGGATACCTAATACCTCAACCAAACATTCCTAAATGGTGGGTTTGGGGCTACTATATTTGCCCTTTATCGTGGTCCTTAAAAGGTATTCTCACTTCACAGTACGGAGATCTAAACACGGAAATAGTACTCCGCGGGGAAGAAAAGACGATCAGTGCCTTCTTAGACAGCATCTATGGATACAACTATGATGATCTAGGAATTGTAGGAATTTTTCTTCTTGCATATCCACTAGTTTTCGCATTTGCGTTTGCACTTGCTACTGAAAAACTAAACTTCCAGAGGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1436

Amino Acids

162.71

Weight (kDa)

7.27

Isoelectric Point (pI)

39.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 173 - 354 2e-15 ABC transporter
ABC2_membrane PF01061 509 - 720 2.4e-41 ABC-2 type transporter
PDR_assoc PF08370 725 - 787 8.9e-20 Plant PDR ABC transporter associated
ABC_tran PF00005 864 - 1016 5.5e-20 ABC transporter
ABC2_membrane PF01061 1161 - 1375 2.6e-56 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000472)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15230 AT4G15230 AT4G15230 AT4G15230 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15236
fragaria_vesca FvH4_4g21360 FvH4_4g21360 FvH4_4g21390 FvH4_6g49431 FvH4_6g49450
malus_domestica MD09G1044300.v1.1 MD16G1137900.v1.1 MD16G1138000.v1.1
prunus_persica Prupe.1G106800_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.3G273300_v2.0.a1
pyrus_communis pycom111g03460 pycom16g12210
rosa_chinensis RchiOBHm_Chr2g0169331 RchiOBHm_Chr2g0169361 RchiOBHm_Chr2g0169371 RchiOBHm_Chr2g0169381 RchiOBHm_Chr2g0169411 RchiOBHm_Chr4g0427141 RchiOBHm_Chr4g0427321
rosa_laevigata RLG00000007252 RLG00000007258 RLG00000013974 RLG00000021856 RLG00000021857 RLG00000021858 RLG00000021859
rosa_multiflora Rmu_sc0000648.1_g000007 Rmu_sc0000648.1_g000009 Rmu_sc0003813.1_g000016 Rmu_sc0009165.1_g000008 Rmu_sc0022554.1_g000001
rosa_roxburghii Rroxscaffold_2G00082200 Rroxscaffold_2G00082230 Rroxscaffold_5G00368960 Rroxscaffold_5G00369010 Rroxscaffold_7G00200390
rosa_rugosa Rorug02G0539500 Rorug02G0539600 Rorug02G0539700 Rorug04G0216400 Rorug04G0216500 Rorug04G0216500 Rorug04G0216600
rosa_samantha Rh2AG610800 Rh2AG611000 Rh2AG611100 Rh2CG592800 Rh2CG593000 Rh2CG593200 Rh2DG634900 Rh2DG635100 Rh2DG635200 Rh2DG635400 Rh4AG272200 Rh4AG272900 Rh4DG275600 Rh4DG276000
rosa_wichuraiana Rw2G050640 Rw2G050660 Rw2G050670 Rw2G050680 Rw4G023610 Rw4G023680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 666
AasI GACNNNNNNGTC 1 cut(s) 1421
Acc36I ACCTGC 1 cut(s) 2095
AccB1I GGYRCC 1 cut(s) 1353
AccB7I CCANNNNNTGG 4 cut(s) 581, 1321, 3473, 3857
AccI GTMKAC 2 cut(s) 1227, 1788
AccII CGCG 1 cut(s) 4155
AciI CCGC 4 cut(s) 124, 2412, 4153, 4155
AclWI GGATC 4 cut(s) 922, 1344, 2023, 3658
AcoI YGGCCR 1 cut(s) 2206
AcsI RAATTY 7 cut(s) 383, 513, 984, 2156, 2372, 2397, 4230
AcuI CTGAAG 9 cut(s) 60, 516, 1145, 1233, 1853, 1906, 2405, 3622, 3963
AhdI GACNNNNNGTC 1 cut(s) 1763
AhlI ACTAGT 2 cut(s) 1618, 4252
AjnI CCWGG 8 cut(s) 252, 574, 774, 2301, 2338, 2617, 2947, 3543
Alw21I GWGCWC 2 cut(s) 74, 3592
Alw26I GTCTC 4 cut(s) 947, 1169, 2340, 2709
AlwI GGATC 4 cut(s) 922, 1344, 2023, 3658
AlwNI CAGNNNCTG 2 cut(s) 2018, 2551
Ama87I CYCGRG 1 cut(s) 4021
AoxI GGCC 5 cut(s) 873, 1235, 2023, 2124, 2206
ApeKI GCWGC 8 cut(s) 11, 131, 1114, 1823, 3065, 3068, 3566, 3916
ApoI RAATTY 7 cut(s) 383, 513, 984, 2156, 2372, 2397, 4230
AseI ATTAAT 1 cut(s) 2223
Asp700I GAANNNNTTC 5 cut(s) 162, 411, 1554, 2002, 2898
AspLEI GCGC 1 cut(s) 3726
AspS9I GGNCC 6 cut(s) 87, 569, 967, 1235, 1993, 4095
AsuHPI GGTGA 3 cut(s) 1031, 3635, 3802
AsuII TTCGAA 1 cut(s) 166
AvaI CYCGRG 1 cut(s) 4021
AvaII GGWCC 5 cut(s) 87, 569, 967, 1993, 4095
BalI TGGCCA 1 cut(s) 2208
BanI GGYRCC 1 cut(s) 1353
BanII GRGCYC 1 cut(s) 2499
BarI GAAGNNNNNNTAC 4 cut(s) 154, 186, 4099, 4131
BauI CACGAG 1 cut(s) 1946
BbsI GAAGAC 2 cut(s) 698, 1759
Bbv12I GWGCWC 2 cut(s) 74, 3592
BbvI GCAGC 7 cut(s) 118, 1126, 1810, 3055, 3077, 3553, 3903
BccI CCATC 9 cut(s) 1201, 1835, 2284, 2632, 2665, 2860, 3025, 3471, 3859
BcgI CGANNNNNNTGC 2 cut(s) 1652, 1686
BciT130I CCWGG 8 cut(s) 254, 576, 776, 2303, 2340, 2619, 2949, 3545
BciVI GTATCC 2 cut(s) 4019, 4193
BclI TGATCA 2 cut(s) 202, 3792
BcoDI GTCTC 4 cut(s) 947, 1169, 2340, 2709
BcuI ACTAGT 2 cut(s) 1618, 4252
BfmI CTRYAG 5 cut(s) 12, 1112, 1372, 2582, 3330
BfuAI ACCTGC 1 cut(s) 2095
BfuI GTATCC 2 cut(s) 4019, 4193
BglII AGATCT 2 cut(s) 1665, 4129
BisI GCNGC 8 cut(s) 12, 132, 1115, 1824, 3066, 3069, 3567, 3917
BlpI GCTNAGC 2 cut(s) 1036, 3360
BlsI GCNGC 8 cut(s) 13, 133, 1116, 1825, 3067, 3070, 3568, 3918
BmcAI AGTACT 4 cut(s) 1349, 1679, 2898, 4149
Bme1390I CCNGG 8 cut(s) 254, 576, 776, 2303, 2340, 2619, 2949, 3545
Bme18I GGWCC 5 cut(s) 87, 569, 967, 1993, 4095
BmeRI GACNNNNNGTC 1 cut(s) 1763
BmeT110I CYCGRG 1 cut(s) 4021
BmgT120I GGNCC 6 cut(s) 87, 569, 967, 1235, 1993, 4095
BmiI GGNNCC 4 cut(s) 968, 969, 1355, 3270
BmrFI CCNGG 8 cut(s) 254, 576, 776, 2303, 2340, 2619, 2949, 3545
BmsI GCATC 9 cut(s) 65, 476, 543, 1846, 1960, 1994, 3046, 3578, 4200
BpiI GAAGAC 2 cut(s) 698, 1759
BpmI CTGGAG 4 cut(s) 558, 2322, 2784, 3544
Bpu10I CCTNAGC 1 cut(s) 1280
Bpu1102I GCTNAGC 2 cut(s) 1036, 3360
Bpu14I TTCGAA 1 cut(s) 166
BpuEI CTTGAG 2 cut(s) 269, 617
Bsa29I ATCGAT 1 cut(s) 3854
BsaAI YACGTR 2 cut(s) 1129, 1643
BsaI GGTCTC 1 cut(s) 1169
BsaJI CCNNGG 9 cut(s) 252, 775, 931, 1231, 1989, 2168, 2302, 3544, 4153
BsaXI ACNNNNNCTCC 2 cut(s) 3183, 3213
Bse1I ACTGG 6 cut(s) 759, 1355, 2608, 2701, 3527, 3872
Bse3DI GCAATG 4 cut(s) 1611, 1729, 1847, 3733
BseBI CCWGG 8 cut(s) 254, 576, 776, 2303, 2340, 2619, 2949, 3545
BseCI ATCGAT 1 cut(s) 3854
BseDI CCNNGG 9 cut(s) 252, 775, 931, 1231, 1989, 2168, 2302, 3544, 4153
BseMI GCAATG 4 cut(s) 1611, 1729, 1847, 3733
BseNI ACTGG 6 cut(s) 759, 1355, 2608, 2701, 3527, 3872
BseRI GAGGAG 2 cut(s) 3204, 3754
BseXI GCAGC 7 cut(s) 118, 1126, 1810, 3055, 3077, 3553, 3903
BseYI CCCAGC 2 cut(s) 3217, 3295
Bsh1236I CGCG 1 cut(s) 4155
BshFI GGCC 5 cut(s) 875, 1237, 2025, 2126, 2208
BshNI GGYRCC 1 cut(s) 1353
BshVI ATCGAT 1 cut(s) 3854
BsiHKAI GWGCWC 2 cut(s) 74, 3592
BsiHKCI CYCGRG 1 cut(s) 4021
BsiSI CCGG 1 cut(s) 1820
BslFI GGGAC 3 cut(s) 361, 827, 980
BsmAI GTCTC 4 cut(s) 947, 1169, 2340, 2709
BsmFI GGGAC 3 cut(s) 361, 827, 980
BsmI GAATGC 2 cut(s) 3161, 3642
BsnI GGCC 5 cut(s) 875, 1237, 2025, 2126, 2208
Bso31I GGTCTC 1 cut(s) 1169
BsoBI CYCGRG 1 cut(s) 4021
Bsp119I TTCGAA 1 cut(s) 166
Bsp1286I GDGCHC 3 cut(s) 74, 2499, 3592
Bsp1720I GCTNAGC 2 cut(s) 1036, 3360
Bsp19I CCATGG 1 cut(s) 1231
BspACI CCGC 4 cut(s) 124, 2412, 4153, 4155
BspANI GGCC 5 cut(s) 875, 1237, 2025, 2126, 2208
BspDI ATCGAT 1 cut(s) 3854
BspFNI CGCG 1 cut(s) 4155
BspHI TCATGA 4 cut(s) 205, 468, 1560, 2365
BspLI GGNNCC 4 cut(s) 968, 969, 1355, 3270
BspMAI CTGCAG 3 cut(s) 16, 1116, 3334
BspMI ACCTGC 1 cut(s) 2095
BspPI GGATC 4 cut(s) 922, 1344, 2023, 3658
BspT104I TTCGAA 1 cut(s) 166
BspT107I GGYRCC 1 cut(s) 1353
BspTNI GGTCTC 1 cut(s) 1169
BsrDI GCAATG 4 cut(s) 1611, 1729, 1847, 3733
BsrI ACTGG 6 cut(s) 759, 1355, 2608, 2701, 3527, 3872
BssECI CCNNGG 9 cut(s) 252, 775, 931, 1231, 1989, 2168, 2302, 3544, 4153
BssNAI GTATAC 1 cut(s) 1228
BssSI CACGAG 1 cut(s) 1946
BssT1I CCWWGG 4 cut(s) 931, 1231, 1989, 2168
Bst1107I GTATAC 1 cut(s) 1228
Bst2BI CACGAG 1 cut(s) 1946
Bst2UI CCWGG 8 cut(s) 254, 576, 776, 2303, 2340, 2619, 2949, 3545
Bst4CI ACNGT 8 cut(s) 344, 365, 742, 1376, 1901, 2812, 3118, 4122
Bst6I CTCTTC 3 cut(s) 182, 1434, 2811
BstBAI YACGTR 2 cut(s) 1129, 1643
BstBI TTCGAA 1 cut(s) 166
BstC8I GCNNGC 9 cut(s) 54, 831, 1485, 1537, 2210, 2214, 2476, 2780, 2849
BstDSI CCRYGG 2 cut(s) 1231, 4153
BstENI CCTNNNNNAGG 1 cut(s) 1316
BstFNI CGCG 1 cut(s) 4155
BstHHI GCGC 1 cut(s) 3726
BstMAI GTCTC 4 cut(s) 947, 1169, 2340, 2709
BstMWI GCNNNNNNNGC 7 cut(s) 786, 1462, 2494, 2834, 2983, 3065, 3344
BstNI CCWGG 8 cut(s) 254, 576, 776, 2303, 2340, 2619, 2949, 3545
BstNSI RCATGY 4 cut(s) 56, 1487, 1539, 3498
BstSCI CCNGG 8 cut(s) 252, 574, 774, 2301, 2338, 2617, 2947, 3543
BstSFI CTRYAG 5 cut(s) 12, 1112, 1372, 2582, 3330
BstSNI TACGTA 1 cut(s) 1643
BstUI CGCG 1 cut(s) 4155
BstV1I GCAGC 7 cut(s) 118, 1126, 1810, 3055, 3077, 3553, 3903
BstV2I GAAGAC 2 cut(s) 698, 1759
BstX2I RGATCY 4 cut(s) 927, 1665, 2015, 4129
BstXI CCANNNNNNTGG 3 cut(s) 438, 782, 3303
BstYI RGATCY 4 cut(s) 927, 1665, 2015, 4129
BstZ17I GTATAC 1 cut(s) 1228
Bsu15I ATCGAT 1 cut(s) 3854
BsuI GTATCC 2 cut(s) 4019, 4193
BsuRI GGCC 5 cut(s) 875, 1237, 2025, 2126, 2208
BsuTUI ATCGAT 1 cut(s) 3854
BtgI CCRYGG 2 cut(s) 1231, 4153
BtsI GCAGTG 1 cut(s) 3561
BtsIMutI CAGTG 9 cut(s) 349, 535, 752, 1508, 2633, 2666, 3561, 3865, 4180
BveI ACCTGC 1 cut(s) 2095
Cac8I GCNNGC 9 cut(s) 54, 831, 1485, 1537, 2210, 2214, 2476, 2780, 2849
CaiI CAGNNNCTG 2 cut(s) 2018, 2551
CciI TCATGA 4 cut(s) 205, 468, 1560, 2365
CfoI GCGC 1 cut(s) 3726
Cfr13I GGNCC 6 cut(s) 87, 569, 967, 1235, 1993, 4095
Cfr42I CCGCGG 1 cut(s) 4156
ClaI ATCGAT 1 cut(s) 3854
CsiI ACCWGGT 1 cut(s) 2947
DraI TTTAAA 1 cut(s) 3490
DrdI GACNNNNNNGTC 1 cut(s) 1421
DriI GACNNNNNGTC 1 cut(s) 1763
DseDI GACNNNNNNGTC 1 cut(s) 1421
EaeI YGGCCR 1 cut(s) 2206
Eam1104I CTCTTC 3 cut(s) 182, 1434, 2811
Eam1105I GACNNNNNGTC 1 cut(s) 1763
EarI CTCTTC 3 cut(s) 182, 1434, 2811
Eco105I TACGTA 1 cut(s) 1643
Eco130I CCWWGG 4 cut(s) 931, 1231, 1989, 2168
Eco24I GRGCYC 1 cut(s) 2499
Eco31I GGTCTC 1 cut(s) 1169
Eco32I GATATC 3 cut(s) 1065, 1740, 2262
Eco47I GGWCC 5 cut(s) 87, 569, 967, 1993, 4095
Eco57I CTGAAG 9 cut(s) 60, 516, 1145, 1233, 1853, 1906, 2405, 3622, 3963
Eco88I CYCGRG 1 cut(s) 4021
EcoNI CCTNNNNNAGG 1 cut(s) 1316
EcoO109I RGGNCCY 1 cut(s) 967
EcoRII CCWGG 8 cut(s) 252, 574, 774, 2301, 2338, 2617, 2947, 3543
EcoRV GATATC 3 cut(s) 1065, 1740, 2262
EcoT14I CCWWGG 4 cut(s) 931, 1231, 1989, 2168
EcoT22I ATGCAT 2 cut(s) 58, 1861
EcoT38I GRGCYC 1 cut(s) 2499
ErhI CCWWGG 4 cut(s) 931, 1231, 1989, 2168
FalI AAGNNNNNCTT 8 cut(s) 1431, 1463, 1524, 1556, 2883, 2915, 3327, 3359
FaqI GGGAC 3 cut(s) 361, 827, 980
FauI CCCGC 1 cut(s) 4148
FauNDI CATATG 2 cut(s) 2755, 2904
FbaI TGATCA 2 cut(s) 202, 3792
FblI GTMKAC 2 cut(s) 1227, 1788
Fnu4HI GCNGC 8 cut(s) 12, 132, 1115, 1824, 3066, 3069, 3567, 3917
FriOI GRGCYC 1 cut(s) 2499
Fsp4HI GCNGC 8 cut(s) 12, 132, 1115, 1824, 3066, 3069, 3567, 3917
GlaI GCGC 1 cut(s) 3725
GluI GCNGC 8 cut(s) 12, 132, 1115, 1824, 3066, 3069, 3567, 3917
GsaI CCCAGC 2 cut(s) 3221, 3299
GsuI CTGGAG 4 cut(s) 558, 2322, 2784, 3544
HaeIII GGCC 5 cut(s) 875, 1237, 2025, 2126, 2208
HapII CCGG 1 cut(s) 1820
HhaI GCGC 1 cut(s) 3726
Hin6I GCGC 1 cut(s) 3724
HinP1I GCGC 1 cut(s) 3724
HincII GTYRAC 1 cut(s) 1660
HindII GTYRAC 1 cut(s) 1660
HindIII AAGCTT 3 cut(s) 454, 1433, 3236
HinfI GANTC 8 cut(s) 556, 1123, 1136, 1837, 2462, 2819, 3227, 3374
HpaII CCGG 1 cut(s) 1820
HphI GGTGA 3 cut(s) 1031, 3635, 3802
Hpy166II GTNNAC 6 cut(s) 1228, 1660, 1789, 2310, 2702, 3626
Hpy8I GTNNAC 6 cut(s) 1228, 1660, 1789, 2310, 2702, 3626
Hpy99I CGWCG 1 cut(s) 264
HpyCH4III ACNGT 8 cut(s) 344, 365, 742, 1376, 1901, 2812, 3118, 4122
HpyCH4IV ACGT 4 cut(s) 160, 259, 1128, 1642
HpyF10VI GCNNNNNNNGC 7 cut(s) 786, 1462, 2494, 2834, 2983, 3065, 3344
HpySE526I ACGT 4 cut(s) 160, 259, 1128, 1642
HspAI GCGC 1 cut(s) 3724
KflI GGGWCCC 1 cut(s) 967
Ksp22I TGATCA 2 cut(s) 202, 3792
KspI CCGCGG 1 cut(s) 4156
LmnI GCTCC 7 cut(s) 69, 1088, 2170, 2270, 2651, 3481, 3587
Lsp1109I GCAGC 7 cut(s) 118, 1126, 1810, 3055, 3077, 3553, 3903
LweI GCATC 9 cut(s) 65, 476, 543, 1846, 1960, 1994, 3046, 3578, 4200
MabI ACCWGGT 1 cut(s) 2947
MaeII ACGT 4 cut(s) 160, 259, 1128, 1642
MfeI CAATTG 2 cut(s) 882, 1118
MflI RGATCY 4 cut(s) 927, 1665, 2015, 4129
MhlI GDGCHC 3 cut(s) 74, 2499, 3592
MlsI TGGCCA 1 cut(s) 2208
MluNI TGGCCA 1 cut(s) 2208
MlyI GAGTC 3 cut(s) 550, 1117, 2828
MmeI TCCRAC 2 cut(s) 1405, 2076
Mox20I TGGCCA 1 cut(s) 2208
Mph1103I ATGCAT 2 cut(s) 58, 1861
MroXI GAANNNNTTC 5 cut(s) 162, 411, 1554, 2002, 2898
MscI TGGCCA 1 cut(s) 2208
MseI TTAA 8 cut(s) 621, 1410, 2036, 2223, 2276, 3489, 3557, 4100
MslI CAYNNNNRTG 5 cut(s) 51, 866, 1482, 3382, 3499
Msp20I TGGCCA 1 cut(s) 2208
MspA1I CMGCKG 2 cut(s) 3068, 4155
MspI CCGG 1 cut(s) 1820
MspR9I CCNGG 8 cut(s) 254, 576, 776, 2303, 2340, 2619, 2949, 3545
MunI CAATTG 2 cut(s) 882, 1118
Mva1269I GAATGC 2 cut(s) 3161, 3642
MvaI CCWGG 8 cut(s) 254, 576, 776, 2303, 2340, 2619, 2949, 3545
MvnI CGCG 1 cut(s) 4155
MwoI GCNNNNNNNGC 7 cut(s) 786, 1462, 2494, 2834, 2983, 3065, 3344
NcoI CCATGG 1 cut(s) 1231
NdeI CATATG 2 cut(s) 2755, 2904
NlaIV GGNNCC 4 cut(s) 968, 969, 1355, 3270
NmuCI GTSAC 5 cut(s) 1241, 2197, 3316, 3870, 3955
NsiI ATGCAT 2 cut(s) 58, 1861
NspI RCATGY 4 cut(s) 56, 1487, 1539, 3498
NspV TTCGAA 1 cut(s) 166
PaeI GCATGC 3 cut(s) 56, 1487, 1539
PagI TCATGA 4 cut(s) 205, 468, 1560, 2365
PctI GAATGC 2 cut(s) 3161, 3642
PdmI GAANNNNTTC 5 cut(s) 162, 411, 1554, 2002, 2898
PfeI GAWTC 5 cut(s) 1136, 1837, 2462, 3227, 3374
PflMI CCANNNNNTGG 4 cut(s) 581, 1321, 3473, 3857
PkrI GCNGC 8 cut(s) 13, 133, 1116, 1825, 3067, 3070, 3568, 3918
PleI GAGTC 3 cut(s) 550, 1117, 2827
PpsI GAGTC 3 cut(s) 550, 1117, 2827
Ppu21I YACGTR 2 cut(s) 1129, 1643
PpuMI RGGWCCY 1 cut(s) 967
PshBI ATTAAT 1 cut(s) 2223
PsiI TTATAA 1 cut(s) 666
Psp5II RGGWCCY 1 cut(s) 967
Psp6I CCWGG 8 cut(s) 252, 574, 774, 2301, 2338, 2617, 2947, 3543
PspFI CCCAGC 2 cut(s) 3217, 3295
PspGI CCWGG 8 cut(s) 252, 574, 774, 2301, 2338, 2617, 2947, 3543
PspN4I GGNNCC 4 cut(s) 968, 969, 1355, 3270
PspPI GGNCC 6 cut(s) 87, 569, 967, 1235, 1993, 4095
PspPPI RGGWCCY 1 cut(s) 967
PsrI GAACNNNNNNTAC 2 cut(s) 3405, 3437
PstI CTGCAG 3 cut(s) 16, 1116, 3334
PstNI CAGNNNCTG 2 cut(s) 2018, 2551
PsuI RGATCY 4 cut(s) 927, 1665, 2015, 4129
PvuII CAGCTG 1 cut(s) 3068
RseI CAYNNNNRTG 5 cut(s) 51, 866, 1482, 3382, 3499
SacII CCGCGG 1 cut(s) 4156
SaqAI TTAA 8 cut(s) 621, 1410, 2036, 2223, 2276, 3489, 3557, 4100
SatI GCNGC 8 cut(s) 12, 132, 1115, 1824, 3066, 3069, 3567, 3917
Sau96I GGNCC 6 cut(s) 87, 569, 967, 1235, 1993, 4095
ScaI AGTACT 4 cut(s) 1349, 1679, 2898, 4149
SchI GAGTC 3 cut(s) 550, 1117, 2828
ScrFI CCNGG 8 cut(s) 254, 576, 776, 2303, 2340, 2619, 2949, 3545
SduI GDGCHC 3 cut(s) 74, 2499, 3592
SexAI ACCWGGT 1 cut(s) 2947
SfaNI GCATC 9 cut(s) 65, 476, 543, 1846, 1960, 1994, 3046, 3578, 4200
SfcI CTRYAG 5 cut(s) 12, 1112, 1372, 2582, 3330
Sfr303I CCGCGG 1 cut(s) 4156
SfuI TTCGAA 1 cut(s) 166
SgrBI CCGCGG 1 cut(s) 4156
SinI GGWCC 5 cut(s) 87, 569, 967, 1993, 4095
SmiMI CAYNNNNRTG 5 cut(s) 51, 866, 1482, 3382, 3499
SmlI CTYRAG 2 cut(s) 284, 632
SmoI CTYRAG 2 cut(s) 284, 632
SnaBI TACGTA 1 cut(s) 1643
SpeI ACTAGT 2 cut(s) 1618, 4252
SphI GCATGC 3 cut(s) 56, 1487, 1539
SsiI CCGC 4 cut(s) 124, 2412, 4153, 4155
SspI AATATT 1 cut(s) 3657
StyD4I CCNGG 8 cut(s) 252, 574, 774, 2301, 2338, 2617, 2947, 3543
StyI CCWWGG 4 cut(s) 931, 1231, 1989, 2168
TaaI ACNGT 8 cut(s) 344, 365, 742, 1376, 1901, 2812, 3118, 4122
TaiI ACGT 4 cut(s) 163, 262, 1131, 1645
TaqI TCGA 7 cut(s) 166, 2578, 2966, 3165, 3739, 3836, 3854
TatI WGTACW 7 cut(s) 1347, 1597, 1677, 2896, 3104, 3759, 4147
TfiI GAWTC 5 cut(s) 1136, 1837, 2462, 3227, 3374
Tru1I TTAA 8 cut(s) 621, 1410, 2036, 2223, 2276, 3489, 3557, 4100
Tru9I TTAA 8 cut(s) 621, 1410, 2036, 2223, 2276, 3489, 3557, 4100
TscAI CASTG 9 cut(s) 349, 535, 759, 1515, 2640, 2673, 3568, 3872, 4180
TseFI GTSAC 5 cut(s) 1241, 2197, 3316, 3870, 3955
TseI GCWGC 8 cut(s) 11, 131, 1114, 1823, 3065, 3068, 3566, 3916
Tsp45I GTSAC 5 cut(s) 1241, 2197, 3316, 3870, 3955
TspGWI ACGGA 3 cut(s) 3010, 4140, 4154
TspRI CASTG 9 cut(s) 349, 535, 759, 1515, 2640, 2673, 3568, 3872, 4180
Van91I CCANNNNNTGG 4 cut(s) 581, 1321, 3473, 3857
VpaK11BI GGWCC 5 cut(s) 87, 569, 967, 1993, 4095
VspI ATTAAT 1 cut(s) 2223
XagI CCTNNNNNAGG 1 cut(s) 1316
XapI RAATTY 7 cut(s) 383, 513, 984, 2156, 2372, 2397, 4230
XbaI TCTAGA 3 cut(s) 508, 1544, 1754
XceI RCATGY 4 cut(s) 56, 1487, 1539, 3498
XcmI CCANNNNNNNNNTGG 1 cut(s) 3474
XmiI GTMKAC 2 cut(s) 1227, 1788
XmnI GAANNNNTTC 5 cut(s) 162, 411, 1554, 2002, 2898
ZrmI AGTACT 4 cut(s) 1349, 1679, 2898, 4149
Zsp2I ATGCAT 2 cut(s) 58, 1861
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.