Rh4AG272200
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000472
Physical Location & Seq
Forward (+)
58805969 .. 58812683
6715 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG272200.1

Sequence Viewer

Length: 4314 bp
ATGGAGCTAACACAAGTTAAGAGAGACGTCCACTCATCGTTTCGACACCAAGCTTCGAGCTTCGGTGCTGGTTCAGACTCAATCAAAGAGGAGGATGAGGAAACTCAACTGCAGTGGACTGCCATTGAGAGATTGCCTACATTCAGAAGGCTGAGGACCTCACTTTTCGACCTCGGTGATAGCGAAAATGGAAAAACCAGTGGCGAAGAGTGTGCAGGGAAGAAGGTGGTTGATGTTACCAAGTTGGGAGCAGATGAGAGGCATCTTTTCATTGAGAAGATCATAAAGCACATTGAGCATGATAATCTGAGGCTGCTACAAAAAATGAGAGAAAGGATAGACAGAGTGAATGTGAAATTGCCAACTGTGGAGGTGAGGTACAAGAGTTTGTGTGTGGAAGCCGAGTGTGAAGTAGTACAAGGAAAGCCGCTCCCGACGCTGTGGAACTCCCTTCTAAGCTTGTTATCAGTTTTTCGAAAGGTAATGTGGTGCAAGTCTCAAGATGCAAAGATTAGCGTTCTCACAGATGTCAGTGGCGTCATCAAACCTTCAAGGATTACTCTCCTTCTTGGTCCCCCGGGGTGTGGTAAAACCACCTTCTTATTGGCACTTGCCGGAAAACTGGATAAATCCCTGAAGGTTGCAGGGGAGATTTCATACAATGGTTACAAGCTAGATGAGTTTGTTCCTCAAAAAACTTCAGCGTACATAAGCCAACACGACCTTCACGTACCTGAGATGACTGTAAGAGAAACAATCGAGTTTTCAGCCCGCTGTCAAGGTGTTGGAAGCAGAGCTGATATAATGCTAGAGGTTAGCAGGAGAGAAAAGGAAGCAGGAATTGTTCCTGATCCAGATATTGACACCTACATGAAGGCAATCTCAGTTGAAGGACAAAAGAAAAACCTCCAAACTGACTATGTTTTGAAGATGCTAGGACTAGATATCTGCAGTGACACAATGGTTGGTGATGCATTACAAAGAGGTATTTCGGGTGGCCAAAAGAAAAGGCTGACAACAGGAGAGATGATTGTCGGTCCCACAAAAGCGCTATTTATGGATGAAATATCAACTGGATTAGACAGCTCTACAACTTTTCAGATAGTCACATATCTACAGCAGTTGGTGCACATAACAGATACAACTGCATTGGTGTCACTTCTACAACCAGCACCTGAGACATTCGATTTATTTGATGATGTGATACTAATGGCAGAGGGGAAGATAGTTTACCATGGTCCTCGGAGTCATGCACTTCAGTTTTTCGAAGATTGTGGTTTTAAGTGCCCACCAAGAAAGGGTGCTGCAGATTATCTTCAGGAGGTAATCTCAAAGAAGGATCAAGCACAATACTGGAAGCATGATAACATTCCCTATGATTATGTTTCTGTGGACAAATTCTCTCAGCTTTTCAGAGCAAGTTACTTGGGAAAGAAGTTAACTGATGAGCTCTCAAAACCATATGAGAAATCACCGGGCCAGGATAAGGCTTTATCATTTAGCATCTACTCTGTCAGAAAATGGGAATTGTTCAAAGCTTGCATGGGTAGAGAGCTGCTTCTTATGAAACGAAATTCCTATGTTTATGTATTCAAAACAGCGCAGCTTGTCATCACTGCACTTTTGACAACGACAGTATTCATACGTACTCAGATGGATGTGGATTTGACAAGTGCAAATTATTTTATGGGTGCATTGTTCTATACACTTGTGAGACTTATGACCAATGGAGTTGCAGAACTGTCCTTGACTATCACTAGACTACCAGTGGTTGATAAGCAAAGAGCCTTCTATCTTTATCCAGCATGGTCCTATTCAATTCCAGCCTCTCTCCTGAAGATTCCATTTTCACTGAGTGATGCGGTGTTGTGGACAGCAATAACTTACTATGGTGTTGGGTATAGCCCTGAAATAAAAAGGTTCTTCTGCCAGCTCCTTGTGCTGTTTGCTCTGCATCTTGCATCAACATCCATGTGTCGTTTGATTGCAGTAATTTTCCGATCCATGGTTGTTGCAACAACTATTGGTACTTTAATCTTGGTACTAATGTTTGTATTTGGAGGCTTCATTTTGCCACCACCCTCATTACCTGTTTGGTTGAAGTGGGGATTCTGGTGTTCTCCTATGACTTATGGGGAAATAGCTGCAAGTCTGAATGAATTCCGTGCTCCTCGATGGCAAAAGGTTTCAAAGGGAAACACAACCTTGGGGAATGAAATTCTAACCAGTCATGGTTTGAACTTTGATAGCTACTTCTACTGGATATCAGTAGGGGCTTTATTCGGGTTCACAGCTATTTTTGATCTTGGATTTGTTTTAGCCTTAACTTACTTAAAATCTCCAAAGATGTCTCGTGCTATTATTTCGAAAAAAAGATTATCTCAACTGAAAGGACAAGATTCTTGGAACAAGACCGGTGCAAAGTCAGATAATGTATCAACTCCAGCTGATTTTTCCCAAACCATGGAAGAAACAGGGAAGATGGTTCTGCCATTTGAGCCGCTGACAATCTCATTTAAGGATGTGCAGTACAGTGTTGACATCCCACCGGAAATGAAAAGGCACGGTTTCGATCAGAAAAAACTTCAGCTGCTTAAAGATATTACAGGAGCATTTAGGCCGGGAGTTCTTACAGCATTGATGGGAGTCAGTGGGGCTGGGAAAACAACTCTCATGGATGTTCTTTCGGGAAGGAAAACCGGAGGCACTATTGAAGGAGATATAAGAATAGGAGGCTATCCCAAAGTCCAGAAGACATTCGCTAGAATATCAGGTTACTGTGAACAATTTGATATACATTCTCCACACATCACAGTAGGAGAGTCGGTTATGTTTTCAGCTTGGTTGAGGTTGCCACCCGAAATTGATCAAGACACAAAAGCTAAATTTGTAGAAGAAGTTATTGAAACAATTGAATTGGAGGATATAAGAGATTCTTTAGTTGGCATTCCTGGCCAAAGTGGTCTATCAACCGAACAGCGCAAAAGGCTAACAATTGCAGTTGAGCTTGTTTCCAATCCATCAATAATATTTATGGATGAACCTACATCAGGACTAGATGCCAGAGCAGCGGCAATTGTCATGCGTGCTGTGAAGAATGTAGTTGCCACAGGAAGAACAACTATTTGCACAATCCACCAACCTAGCATTGATATCTTTGAGGCTTTTGATGAGTTAATTCTGATGAAAACGGGAGGGCAAATTATCTATTCTGGAAGTCTGGGCCATCATTCGAGTAATCTCATTGAATATTTTGAGGGTATTCCTGGTGTACCAAAGATCAAAGATAACTATAACCCCGCAACATGGATGTTAGAAGTTACTTCCTCTTCAGTAGAAGCAGAACTCGGTTTAGATTTTGCGAACATTTATAAAGAATCTACTCTGTATAAAGACACAGTTGAATTGGTGAAACAGATAAGTGAACCAAAGCCAGGTTCAAGAGCTTTGCAATTTTCCACCCAATTTCCACAAAATAGCTGGGTGCAGTTTAGGGCATGCTTATGGAAACAACACTTATCCTACTGGAGAAGTCCTGAATACAATCTAGCACGTTTCATGTTCATGACTGTTGCCTCAGTGTTGTTTGGTGCAATCTTCTGGCAAAAGGGGAAGGAAATAAATAATGAGCAGGATTTGCTGACTATACTTGGGTCCATGTACATTGCTGTAATATTCCTGGGAATAAACAATTGTTCAACAGTTCTTCCTTATGTGGCAACTGAGCGCACCGTTTTGTACCGCGAAAGATTTGCTGGGATGTACTCATCAAAGGCTTATTCCTTTGCACAGGTGGCCATGGAAATACCTTATACAATGTTGCAAGCAATTTTATATGTGGTCATTACATATCCTACGATAGGCTACTATTGGACTGCTTCCAAGGTTTTTTGGTACTTCTATGCAACCTTCTGTACATTTCTATACTTTGTATACCTTGGGATGTTGTTAGTTTCTTTGAGCTCAGACATGGAAGTGGCTTCGATATTGGCCACTGCAATCTACACCATATTGAATCTTTTCTCAGGCTTCCTCATGCCTGGACCGAAAATTCCGAAGTGGTGGATTTGGTGCTACTGGATCTGTCCTACATCATGGTCACTAAATGGCCTCCTGACTTCACAATATGGAGACATGGACAAAGAGATATTGATTTTTGGAGAGCATAAAACAGTTGCTTCCTTCCTACAAGATTATTATGGTTTTCATCATGATCTTTTACCCCTTGTGGCTATTGCTCTGATTACCTTCCCAATTGCTTTTGCTTTTCTCTTTGCCTATTTTATAGGGAGATTAAATTTCCAAAGGCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1437

Amino Acids

161.92

Weight (kDa)

8.01

Isoelectric Point (pI)

37.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 174 - 355 1.6e-16 ABC transporter
ABC2_membrane PF01061 510 - 721 4.3e-45 ABC-2 type transporter
PDR_assoc PF08370 726 - 788 1.2e-23 Plant PDR ABC transporter associated
ABC_tran PF00005 865 - 1017 2.2e-20 ABC transporter
ABC2_membrane PF01061 1163 - 1376 3.4e-58 ABC-2 type transporter
ABC2_membrane_3 PF12698 1213 - 1421 8.8e-07 ABC-2 family transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000472)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15230 AT4G15230 AT4G15230 AT4G15230 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15236
fragaria_vesca FvH4_4g21360 FvH4_4g21360 FvH4_4g21390 FvH4_6g49431 FvH4_6g49450
malus_domestica MD09G1044300.v1.1 MD16G1137900.v1.1 MD16G1138000.v1.1
prunus_persica Prupe.1G106800_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.3G273300_v2.0.a1
pyrus_communis pycom111g03460 pycom16g12210
rosa_chinensis RchiOBHm_Chr2g0169331 RchiOBHm_Chr2g0169361 RchiOBHm_Chr2g0169371 RchiOBHm_Chr2g0169381 RchiOBHm_Chr2g0169411 RchiOBHm_Chr4g0427141 RchiOBHm_Chr4g0427321
rosa_laevigata RLG00000007252 RLG00000007258 RLG00000013974 RLG00000021856 RLG00000021857 RLG00000021858 RLG00000021859
rosa_multiflora Rmu_sc0000648.1_g000007 Rmu_sc0000648.1_g000009 Rmu_sc0003813.1_g000016 Rmu_sc0009165.1_g000008 Rmu_sc0022554.1_g000001
rosa_roxburghii Rroxscaffold_2G00082200 Rroxscaffold_2G00082230 Rroxscaffold_5G00368960 Rroxscaffold_5G00369010 Rroxscaffold_7G00200390
rosa_rugosa Rorug02G0539500 Rorug02G0539600 Rorug02G0539700 Rorug04G0216400 Rorug04G0216500 Rorug04G0216500 Rorug04G0216600
rosa_samantha Rh2AG610800 Rh2AG611000 Rh2AG611100 Rh2CG592800 Rh2CG593000 Rh2CG593200 Rh2DG634900 Rh2DG635100 Rh2DG635200 Rh2DG635400 Rh4AG272200 Rh4AG272900 Rh4DG275600 Rh4DG276000
rosa_wichuraiana Rw2G050640 Rw2G050660 Rw2G050670 Rw2G050680 Rw4G023610 Rw4G023680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3372
AatII GACGTC 1 cut(s) 30
AccB7I CCANNNNNTGG 1 cut(s) 2464
AccBSI CCGCTC 1 cut(s) 430
AccI GTMKAC 1 cut(s) 3933
AccII CGCG 1 cut(s) 3744
AciI CCGC 7 cut(s) 428, 772, 1862, 2501, 3071, 3300, 3742
AclWI GGATC 4 cut(s) 845, 1347, 1995, 4088
AcoI YGGCCR 4 cut(s) 997, 2953, 3795, 3990
AcsI RAATTY 7 cut(s) 1397, 1573, 2159, 2217, 2885, 4050, 4297
AcuI CTGAAG 7 cut(s) 656, 684, 1241, 1301, 1856, 2570, 3315
AcyI GRCGYC 2 cut(s) 27, 537
AdeI CACNNNGTG 1 cut(s) 1856
AfeI AGCGCT 1 cut(s) 1050
AfiI CCNNNNNNNGG 9 cut(s) 584, 1298, 1486, 2005, 2464, 3050, 3306, 3434, 3860
AgeI ACCGGT 1 cut(s) 2414
AjnI CCWGG 6 cut(s) 1479, 2950, 3265, 3433, 3678, 4039
AjuI GAANNNNNNNTTGG 4 cut(s) 708, 740, 3421, 3453
Alw21I GWGCWC 4 cut(s) 1131, 1452, 2170, 3965
Alw26I GTCTC 6 cut(s) 18, 501, 1172, 1708, 2355, 4125
Alw44I GTGCAC 1 cut(s) 1127
AlwI GGATC 4 cut(s) 845, 1347, 1995, 4088
AlwNI CAGNNNCTG 1 cut(s) 1175
Ama87I CYCGRG 1 cut(s) 577
Aor51HI AGCGCT 1 cut(s) 1050
AoxI GGCC 8 cut(s) 997, 1477, 2618, 2953, 3223, 3795, 3990, 4108
ApaLI GTGCAC 1 cut(s) 1127
ApeKI GCWGC 7 cut(s) 313, 1304, 1555, 1603, 2144, 2590, 3068
ApoI RAATTY 7 cut(s) 1397, 1573, 2159, 2217, 2885, 4050, 4297
AsiGI ACCGGT 1 cut(s) 2414
Asp700I GAANNNNTTC 3 cut(s) 2159, 2756, 4019
AspLEI GCGC 4 cut(s) 1051, 1603, 2982, 3729
AspS9I GGNCC 9 cut(s) 156, 572, 1037, 1238, 1477, 1809, 3223, 3654, 4043
AsuC2I CCSGG 4 cut(s) 578, 579, 1476, 2622
AsuHPI GGTGA 5 cut(s) 188, 385, 980, 1464, 3421
AsuII TTCGAA 3 cut(s) 475, 1266, 2366
AvaI CYCGRG 1 cut(s) 577
AvaII GGWCC 7 cut(s) 156, 572, 1037, 1238, 1809, 3654, 4043
BaeGI GKGCMC 2 cut(s) 1131, 1289
BaeI ACNNNNGTAYC 4 cut(s) 370, 403, 2033, 2066
BalI TGGCCA 4 cut(s) 999, 2955, 3797, 3992
BanII GRGCYC 2 cut(s) 1452, 3965
BarI GAAGNNNNNNTAC 2 cut(s) 1214, 1246
BauI CACGAG 1 cut(s) 2352
BbsI GAAGAC 1 cut(s) 2759
Bbv12I GWGCWC 4 cut(s) 1131, 1452, 2170, 3965
BbvCI CCTCAGC 1 cut(s) 152
BbvI GCAGC 7 cut(s) 300, 1291, 1542, 1615, 2131, 2577, 3080
BccI CCATC 6 cut(s) 1648, 2169, 2476, 2635, 3028, 3234
BcgI CGANNNNNNTGC 4 cut(s) 194, 228, 3734, 3768
BciT130I CCWGG 6 cut(s) 1481, 2952, 3267, 3435, 3680, 4041
BclI TGATCA 1 cut(s) 2866
BcnI CCSGG 4 cut(s) 578, 579, 1476, 2622
BcoDI GTCTC 6 cut(s) 18, 501, 1172, 1708, 2355, 4125
BfaI CTAG 9 cut(s) 674, 809, 935, 941, 1758, 2763, 3056, 3144, 3548
BfmI CTRYAG 4 cut(s) 110, 949, 1115, 1305
BfoI RGCGCY 1 cut(s) 1052
Bme18I GGWCC 7 cut(s) 156, 572, 1037, 1238, 1809, 3654, 4043
BmeT110I CYCGRG 1 cut(s) 577
BmgT120I GGNCC 9 cut(s) 156, 572, 1037, 1238, 1477, 1809, 3223, 3654, 4043
BmiI GGNNCC 3 cut(s) 574, 1039, 3655
BmsI GCATC 9 cut(s) 271, 493, 921, 961, 1512, 1849, 1963, 1970, 3049
BpiI GAAGAC 1 cut(s) 2759
BplI GAGNNNNNCTC 2 cut(s) 1313, 1345
BpmI CTGGAG 2 cut(s) 2427, 3547
Bpu10I CCTNAGC 1 cut(s) 152
Bpu14I TTCGAA 3 cut(s) 475, 1266, 2366
BpuEI CTTGAG 1 cut(s) 483
BpuMI CCSGG 4 cut(s) 578, 579, 1476, 2622
BsaAI YACGTR 2 cut(s) 730, 1646
BsaHI GRCGYC 2 cut(s) 27, 537
BsaWI WCCGGW 3 cut(s) 2414, 2548, 2699
BsaXI ACNNNNNCTCC 4 cut(s) 362, 392, 2811, 2841
Bsc4I CCNNNNNNNGG 9 cut(s) 584, 1298, 1486, 2005, 2464, 3050, 3306, 3434, 3860
Bse118I RCCGGY 1 cut(s) 2414
Bse1I ACTGG 9 cut(s) 198, 627, 1078, 1358, 1766, 2226, 2264, 3530, 4082
Bse3DI GCAATG 1 cut(s) 3663
BseBI CCWGG 6 cut(s) 1481, 2952, 3267, 3435, 3680, 4041
BseLI CCNNNNNNNGG 9 cut(s) 584, 1298, 1486, 2005, 2464, 3050, 3306, 3434, 3860
BseMI GCAATG 1 cut(s) 3663
BseNI ACTGG 9 cut(s) 198, 627, 1078, 1358, 1766, 2226, 2264, 3530, 4082
BseRI GAGGAG 2 cut(s) 104, 2160
BseSI GKGCMC 2 cut(s) 1131, 1289
BseXI GCAGC 7 cut(s) 300, 1291, 1542, 1615, 2131, 2577, 3080
BseYI CCCAGC 3 cut(s) 2657, 3480, 3755
BsgI GTGCAG 4 cut(s) 234, 1602, 2546, 3506
Bsh1236I CGCG 1 cut(s) 3744
BshFI GGCC 8 cut(s) 999, 1479, 2620, 2955, 3225, 3797, 3992, 4110
BshTI ACCGGT 1 cut(s) 2414
BsiHKAI GWGCWC 4 cut(s) 1131, 1452, 2170, 3965
BsiHKCI CYCGRG 1 cut(s) 577
BsiSI CCGG 7 cut(s) 578, 615, 1475, 2415, 2549, 2621, 2700
BslFI GGGAC 2 cut(s) 558, 1023
BslI CCNNNNNNNGG 9 cut(s) 584, 1298, 1486, 2005, 2464, 3050, 3306, 3434, 3860
BsmAI GTCTC 6 cut(s) 18, 501, 1172, 1708, 2355, 4125
BsmBI CGTCTC 1 cut(s) 18
BsmFI GGGAC 2 cut(s) 558, 1023
BsmI GAATGC 1 cut(s) 2946
BsnI GGCC 8 cut(s) 999, 1479, 2620, 2955, 3225, 3797, 3992, 4110
BsoBI CYCGRG 1 cut(s) 577
Bsp119I TTCGAA 3 cut(s) 475, 1266, 2366
Bsp1286I GDGCHC 5 cut(s) 1131, 1289, 1452, 2170, 3965
Bsp1407I TGTACA 2 cut(s) 3660, 3914
Bsp19I CCATGG 4 cut(s) 1234, 2004, 2463, 3798
BspACI CCGC 7 cut(s) 428, 772, 1862, 2501, 3071, 3300, 3742
BspANI GGCC 8 cut(s) 999, 1479, 2620, 2955, 3225, 3797, 3992, 4110
BspFNI CGCG 1 cut(s) 3744
BspHI TCATGA 2 cut(s) 3564, 4210
BspLI GGNNCC 3 cut(s) 574, 1039, 3655
BspMAI CTGCAG 3 cut(s) 114, 953, 1309
BspPI GGATC 4 cut(s) 845, 1347, 1995, 4088
BspT104I TTCGAA 3 cut(s) 475, 1266, 2366
BsrBI CCGCTC 1 cut(s) 430
BsrDI GCAATG 1 cut(s) 3663
BsrFI RCCGGY 1 cut(s) 2414
BsrGI TGTACA 2 cut(s) 3660, 3914
BsrI ACTGG 9 cut(s) 198, 627, 1078, 1358, 1766, 2226, 2264, 3530, 4082
BssAI RCCGGY 1 cut(s) 2414
BssNAI GTATAC 1 cut(s) 3934
BssNI GRCGYC 2 cut(s) 27, 537
BssSI CACGAG 1 cut(s) 2352
BssT1I CCWWGG 7 cut(s) 1234, 2004, 2205, 2463, 3798, 3882, 3937
Bst1107I GTATAC 1 cut(s) 3934
Bst2BI CACGAG 1 cut(s) 2352
Bst2UI CCWGG 6 cut(s) 1481, 2952, 3267, 3435, 3680, 4041
Bst6I CTCTTC 2 cut(s) 201, 3334
BstACI GRCGYC 2 cut(s) 27, 537
BstAPI GCANNNNNTGC 2 cut(s) 1126, 3637
BstAUI TGTACA 2 cut(s) 3660, 3914
BstBAI YACGTR 2 cut(s) 730, 1646
BstBI TTCGAA 3 cut(s) 475, 1266, 2366
BstC8I GCNNGC 6 cut(s) 772, 1540, 1931, 3087, 3499, 3825
BstDSI CCRYGG 4 cut(s) 1234, 2004, 2463, 3798
BstENI CCTNNNNNAGG 2 cut(s) 3048, 3858
BstFNI CGCG 1 cut(s) 3744
BstH2I RGCGCY 1 cut(s) 1052
BstHHI GCGC 4 cut(s) 1051, 1603, 2982, 3729
BstMAI GTCTC 6 cut(s) 18, 501, 1172, 1708, 2355, 4125
BstNI CCWGG 6 cut(s) 1481, 2952, 3267, 3435, 3680, 4041
BstNSI RCATGY 1 cut(s) 3501
BstSFI CTRYAG 4 cut(s) 110, 949, 1115, 1305
BstSLI GKGCMC 2 cut(s) 1131, 1289
BstSNI TACGTA 1 cut(s) 1646
BstUI CGCG 1 cut(s) 3744
BstV1I GCAGC 7 cut(s) 300, 1291, 1542, 1615, 2131, 2577, 3080
BstV2I GAAGAC 1 cut(s) 2759
BstX2I RGATCY 1 cut(s) 4080
BstYI RGATCY 1 cut(s) 4080
BstZ17I GTATAC 1 cut(s) 3934
BsuRI GGCC 8 cut(s) 999, 1479, 2620, 2955, 3225, 3797, 3992, 4110
BtgI CCRYGG 4 cut(s) 1234, 2004, 2463, 3798
BtsI GCAGTG 4 cut(s) 119, 958, 1614, 3993
Cac8I GCNNGC 6 cut(s) 772, 1540, 1931, 3087, 3499, 3825
CaiI CAGNNNCTG 1 cut(s) 1175
CciI TCATGA 2 cut(s) 3564, 4210
CfoI GCGC 4 cut(s) 1051, 1603, 2982, 3729
Cfr10I RCCGGY 1 cut(s) 2414
Cfr13I GGNCC 9 cut(s) 156, 572, 1037, 1238, 1477, 1809, 3223, 3654, 4043
Cfr9I CCCGGG 1 cut(s) 577
CseI GACGC 2 cut(s) 445, 526
CspAI ACCGGT 1 cut(s) 2414
CspCI CAANNNNNGTGG 2 cut(s) 95, 130
DraIII CACNNNGTG 1 cut(s) 1856
EaeI YGGCCR 4 cut(s) 997, 2953, 3795, 3990
Eam1104I CTCTTC 2 cut(s) 201, 3334
EarI CTCTTC 2 cut(s) 201, 3334
Ecl136II GAGCTC 2 cut(s) 1450, 3963
Eco105I TACGTA 1 cut(s) 1646
Eco130I CCWWGG 7 cut(s) 1234, 2004, 2205, 2463, 3798, 3882, 3937
Eco24I GRGCYC 2 cut(s) 1452, 3965
Eco32I GATATC 3 cut(s) 946, 2265, 3154
Eco47I GGWCC 7 cut(s) 156, 572, 1037, 1238, 1809, 3654, 4043
Eco47III AGCGCT 1 cut(s) 1050
Eco53kI GAGCTC 2 cut(s) 1450, 3963
Eco57I CTGAAG 7 cut(s) 656, 684, 1241, 1301, 1856, 2570, 3315
Eco88I CYCGRG 1 cut(s) 577
EcoICRI GAGCTC 2 cut(s) 1450, 3963
EcoNI CCTNNNNNAGG 2 cut(s) 3048, 3858
EcoO109I RGGNCCY 1 cut(s) 156
EcoRI GAATTC 1 cut(s) 2159
EcoRII CCWGG 6 cut(s) 1479, 2950, 3265, 3433, 3678, 4039
EcoRV GATATC 3 cut(s) 946, 2265, 3154
EcoT14I CCWWGG 7 cut(s) 1234, 2004, 2205, 2463, 3798, 3882, 3937
EcoT22I ATGCAT 1 cut(s) 976
EcoT38I GRGCYC 2 cut(s) 1452, 3965
ErhI CCWWGG 7 cut(s) 1234, 2004, 2205, 2463, 3798, 3882, 3937
Esp3I CGTCTC 1 cut(s) 18
FalI AAGNNNNNCTT 2 cut(s) 2920, 2952
FaqI GGGAC 2 cut(s) 558, 1023
FauI CCCGC 2 cut(s) 779, 3307
FauNDI CATATG 1 cut(s) 1462
FbaI TGATCA 1 cut(s) 2866
FblI GTMKAC 1 cut(s) 3933
FriOI GRGCYC 2 cut(s) 1452, 3965
FspBI CTAG 9 cut(s) 674, 809, 935, 941, 1758, 2763, 3056, 3144, 3548
GlaI GCGC 4 cut(s) 1050, 1602, 2981, 3728
GsaI CCCAGC 3 cut(s) 2661, 3484, 3759
GsuI CTGGAG 2 cut(s) 2427, 3547
HaeII RGCGCY 1 cut(s) 1052
HaeIII GGCC 8 cut(s) 999, 1479, 2620, 2955, 3225, 3797, 3992, 4110
HapII CCGG 7 cut(s) 578, 615, 1475, 2415, 2549, 2621, 2700
HgaI GACGC 2 cut(s) 445, 526
HhaI GCGC 4 cut(s) 1051, 1603, 2982, 3729
Hin1I GRCGYC 2 cut(s) 27, 537
Hin6I GCGC 4 cut(s) 1049, 1601, 2980, 3727
HinP1I GCGC 4 cut(s) 1049, 1601, 2980, 3727
HincII GTYRAC 2 cut(s) 1440, 2539
HindII GTYRAC 2 cut(s) 1440, 2539
HindIII AAGCTT 3 cut(s) 51, 457, 1536
HpaI GTTAAC 1 cut(s) 1440
HpaII CCGG 7 cut(s) 578, 615, 1475, 2415, 2549, 2621, 2700
HphI GGTGA 5 cut(s) 188, 385, 980, 1464, 3421
Hpy99I CGWCG 1 cut(s) 439
HpyCH4IV ACGT 4 cut(s) 27, 729, 1645, 3553
HpySE526I ACGT 4 cut(s) 27, 729, 1645, 3553
Hsp92I GRCGYC 2 cut(s) 27, 537
HspAI GCGC 4 cut(s) 1049, 1601, 2980, 3727
Ksp22I TGATCA 1 cut(s) 2866
KspAI GTTAAC 1 cut(s) 1440
LmnI GCTCC 6 cut(s) 4, 248, 435, 1938, 2173, 2609
Lsp1109I GCAGC 7 cut(s) 300, 1291, 1542, 1615, 2131, 2577, 3080
LweI GCATC 9 cut(s) 271, 493, 921, 961, 1512, 1849, 1963, 1970, 3049
MaeI CTAG 9 cut(s) 674, 809, 935, 941, 1758, 2763, 3056, 3144, 3548
MaeII ACGT 4 cut(s) 27, 729, 1645, 3553
MaeIII GTNAC 9 cut(s) 235, 665, 953, 1105, 1155, 1421, 2774, 3319, 4098
MbiI CCGCTC 1 cut(s) 430
MfeI CAATTG 5 cut(s) 2910, 2994, 3075, 3691, 4254
MflI RGATCY 1 cut(s) 4080
MhlI GDGCHC 5 cut(s) 1131, 1289, 1452, 2170, 3965
MlsI TGGCCA 4 cut(s) 999, 2955, 3797, 3992
MluNI TGGCCA 4 cut(s) 999, 2955, 3797, 3992
MlyI GAGTC 4 cut(s) 71, 1255, 2655, 2831
MmeI TCCRAC 1 cut(s) 766
Mox20I TGGCCA 4 cut(s) 999, 2955, 3797, 3992
Mph1103I ATGCAT 1 cut(s) 976
MroXI GAANNNNTTC 3 cut(s) 2159, 2756, 4019
MscI TGGCCA 4 cut(s) 999, 2955, 3797, 3992
MslI CAYNNNNRTG 5 cut(s) 869, 1972, 3502, 3563, 3974
Msp20I TGGCCA 4 cut(s) 999, 2955, 3797, 3992
MspA1I CMGCKG 5 cut(s) 774, 2447, 2503, 2590, 3071
MspI CCGG 7 cut(s) 578, 615, 1475, 2415, 2549, 2621, 2700
MunI CAATTG 5 cut(s) 2910, 2994, 3075, 3691, 4254
Mva1269I GAATGC 1 cut(s) 2946
MvaI CCWGG 6 cut(s) 1481, 2952, 3267, 3435, 3680, 4041
MvnI CGCG 1 cut(s) 3744
NciI CCSGG 4 cut(s) 578, 579, 1476, 2622
NcoI CCATGG 4 cut(s) 1234, 2004, 2463, 3798
NdeI CATATG 1 cut(s) 1462
NlaIV GGNNCC 3 cut(s) 574, 1039, 3655
NmeAIII GCCGAG 1 cut(s) 427
NmuCI GTSAC 4 cut(s) 953, 1105, 1155, 4098
NsiI ATGCAT 1 cut(s) 976
NspI RCATGY 1 cut(s) 3501
NspV TTCGAA 3 cut(s) 475, 1266, 2366
PaeI GCATGC 1 cut(s) 3501
PagI TCATGA 2 cut(s) 3564, 4210
PcsI WCGNNNNNNNCGW 2 cut(s) 180, 726
PctI GAATGC 1 cut(s) 2946
PdmI GAANNNNTTC 3 cut(s) 2159, 2756, 4019
PfeI GAWTC 6 cut(s) 1840, 2109, 2399, 2933, 3377, 4015
PflMI CCANNNNNTGG 1 cut(s) 2464
PinAI ACCGGT 1 cut(s) 2414
PleI GAGTC 4 cut(s) 71, 1254, 2654, 2830
PpsI GAGTC 4 cut(s) 71, 1254, 2654, 2830
Ppu21I YACGTR 2 cut(s) 730, 1646
PpuMI RGGWCCY 1 cut(s) 156
PsiI TTATAA 1 cut(s) 3372
Psp124BI GAGCTC 2 cut(s) 1452, 3965
Psp5II RGGWCCY 1 cut(s) 156
Psp6I CCWGG 6 cut(s) 1479, 2950, 3265, 3433, 3678, 4039
PspFI CCCAGC 3 cut(s) 2657, 3480, 3755
PspGI CCWGG 6 cut(s) 1479, 2950, 3265, 3433, 3678, 4039
PspN4I GGNNCC 3 cut(s) 574, 1039, 3655
PspPI GGNCC 9 cut(s) 156, 572, 1037, 1238, 1477, 1809, 3223, 3654, 4043
PspPPI RGGWCCY 1 cut(s) 156
PstI CTGCAG 3 cut(s) 114, 953, 1309
PstNI CAGNNNCTG 1 cut(s) 1175
PsuI RGATCY 1 cut(s) 4080
PvuII CAGCTG 2 cut(s) 2447, 2590
RseI CAYNNNNRTG 5 cut(s) 869, 1972, 3502, 3563, 3974
SacI GAGCTC 2 cut(s) 1452, 3965
Sau96I GGNCC 9 cut(s) 156, 572, 1037, 1238, 1477, 1809, 3223, 3654, 4043
SchI GAGTC 4 cut(s) 71, 1255, 2655, 2831
SduI GDGCHC 5 cut(s) 1131, 1289, 1452, 2170, 3965
SfaNI GCATC 9 cut(s) 271, 493, 921, 961, 1512, 1849, 1963, 1970, 3049
SfcI CTRYAG 4 cut(s) 110, 949, 1115, 1305
SfuI TTCGAA 3 cut(s) 475, 1266, 2366
SinI GGWCC 7 cut(s) 156, 572, 1037, 1238, 1809, 3654, 4043
SmaI CCCGGG 1 cut(s) 579
SmiMI CAYNNNNRTG 5 cut(s) 869, 1972, 3502, 3563, 3974
SmlI CTYRAG 1 cut(s) 498
SmoI CTYRAG 1 cut(s) 498
SnaBI TACGTA 1 cut(s) 1646
SphI GCATGC 1 cut(s) 3501
SsiI CCGC 7 cut(s) 428, 772, 1862, 2501, 3071, 3300, 3742
SspI AATATT 3 cut(s) 3030, 3251, 3675
SspMI CTAG 9 cut(s) 674, 809, 935, 941, 1758, 2763, 3056, 3144, 3548
SstI GAGCTC 2 cut(s) 1452, 3965
StyI CCWWGG 7 cut(s) 1234, 2004, 2205, 2463, 3798, 3882, 3937
TaiI ACGT 4 cut(s) 30, 732, 1648, 3556
TaqII GACCGA 2 cut(s) 1025, 4060
TatI WGTACW 5 cut(s) 415, 2529, 3660, 3762, 3914
TauI GCSGC 3 cut(s) 430, 2503, 3074
TfiI GAWTC 6 cut(s) 1840, 2109, 2399, 2933, 3377, 4015
TseFI GTSAC 4 cut(s) 953, 1105, 1155, 4098
TseI GCWGC 7 cut(s) 313, 1304, 1555, 1603, 2144, 2590, 3068
Tsp45I GTSAC 4 cut(s) 953, 1105, 1155, 4098
TspGWI ACGGA 1 cut(s) 2153
TspMI CCCGGG 1 cut(s) 577
Van91I CCANNNNNTGG 1 cut(s) 2464
VneI GTGCAC 1 cut(s) 1127
VpaK11BI GGWCC 7 cut(s) 156, 572, 1037, 1238, 1809, 3654, 4043
XagI CCTNNNNNAGG 2 cut(s) 3048, 3858
XapI RAATTY 7 cut(s) 1397, 1573, 2159, 2217, 2885, 4050, 4297
XceI RCATGY 1 cut(s) 3501
XcmI CCANNNNNNNNNTGG 2 cut(s) 601, 3477
XmaI CCCGGG 1 cut(s) 577
XmiI GTMKAC 1 cut(s) 3933
XmnI GAANNNNTTC 3 cut(s) 2159, 2756, 4019
XspI CTAG 9 cut(s) 674, 809, 935, 941, 1758, 2763, 3056, 3144, 3548
ZraI GACGTC 1 cut(s) 28
Zsp2I ATGCAT 1 cut(s) 976
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.