Rroxscaffold_7G00200390

pleiotropic drug resistance protein 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000472
Physical Location & Seq
Reverse (-)
47789451 .. 47797810
8360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00200390.1

Sequence Viewer

Length: 4062 bp
ATGGATGCTACAGAGATAGGAAGTTTGAGCACAACATCAAAGCTAGGAGCTCAGGAACGGCGTTTCTTAATTGACAAACTTATCAAAGAAATAGGAGGAGATAACGAACGCCTGTTAAAGAAACTTAGAGAACGAATAGACAGAGTGGGACTAAAATTGCCGACTGTGGAAGTACGGTACAAAAATCTGTTTGTAGAAGCAGACCTTGAAGTAGTTCAAGGAAAGCCCCTCCCTACACTATGGAATACGATCAAAAGCTTTCTCTCTGTGACGAAACGGATAATTGGGTGCAAGTCTCAAGCATACAAACTAGAAATTCTAAAAGATGTCAGTGGCATCATCAAGCCTTCAAGGATGACTCTATTACTTGGTCCTCCAGGCAGTGGGAAAACCACCTTATTACAGGCACTTGCTGGAAAACTTGACCACTCTCTCAAGGTTCAAGGAGAAATAACCTACAATGGTTATCAGCTCAATGAGTTTGTTCCTCAGAAGACATCAGCTTACATAAGTCAACATGACTTGCATATTTCTGAGATGACTGTGAGGGAAGCACTGGATTTCTCATCTCGTTGCCAGGGCATTGGAAGCAGAGCTGATATTATGAAAGAAGTTGCTCGGAGGGAAAAGGAAGCTGGTATTGTCCCAGAACCAGACATTGACACTTACATGAAGGCCATTTCAATTGAGGGGCTGAAAAAATCTCTAATGACAGACTATATAATAAAGATCCTTGGGCTGGACAATTGTGCTGATACAATTGTAGGGGATGCAATGCAAAGAGGAATTTCAGGTGGTCAAAAGAAAAGGCTAACAACAGGTGAAATGATGATTGGCCCAGCAAGAGCTTTGTTCATGGATGAGATATCAACAGGACTAGACAGCTCCACTACATTTCAGATTGTAACTTGTCTGCAGCAATTGACTCACGTAACAGAATCCACCATTTTGGTTTCACTTCTTCAGCCAACGCCAGAGACCTTTCATCTTTTTGATGACCTTATCTTGATGGCAGAAGGAAAAGTTGTATACCATGGGCCTAGTGATAATGTGGTAGAGTTCTTTGAGAACTGTGGTTTCCGTAGCCCACCACGAAAAGGCATTTCTGACTTCCTCCAAGAGGCAAGAGTGGTCTCTCCAAAGGATCAAGCAAAGTACTGGTACCATAAAGACCAACCCCACAGTTATGTTAGTGTGGATGAGTTCGTAAGTATGTTTAAGGACTTCCATGTTGGAAAAAAGCTTGAAGAGGAGCTTTCTAAGCCTTTCGAGCAATCAGAGTGCCACAAAAATGCTTTGTCATACAACCCTTACTCACTGAAAAAATGGGAACTATTCAAAGCATGCCTTTCTAGAGAATGGCTTCTGATGAAGCGAAACTCATTTGTTCATGTATTCCAATCAGCACAGCTTGTGTTCATTGCACTAGTAACAATGACAATGTTTCTACGTACATGGATGAAAGTCGACGAGATCTATGCAAAGTACTACATGAGTGCTTTGTTTTATGCTCTAATTAGATTTCTATGCAATGCAAGTTCAGAGATATCAATGACTTCCTCAAGACTTGCAGTCTTCTACAAGCAGAGAGATTTCTACTTCTACCCTGCATGGGCTTATTCTATTCCGGCTGCCATCCTGAAGATTCCGTTTTCATTGCTAGATGCATTTCTTTGGACAGCCCTTACTTATTATGTGATTGGTTACAGTCCTGAACCTGAAAGGTTCTTCAGGCAATTTATTCTTCTGTTCCTCGTGCATCAAGTAGCAATATCACTATTTCGTTTGATCGCATCCTTGGTCAGAAATCCTTCTGTTGCAGGATCTGTTGGTCTTTTCTCTTTAATAGTAATGTTTCTATTCGGAGGCTTCATAATTCCAAAATCTTCTCTACCTGCTTGGTTGGCATGGGGATTTTGGGTTTCTCCATTGGCCTTTGCAGAAATAGGTGCTTCAATAAATGAATTTCATGCTCCAAGGTGGCAAAAGGTTTTATCTTCCAATGTTACTATAGGCCAGCAAGCTCTAATTAACCGTGGTCTAAACTTCAGTGATTACTTCTATTGGATATCCATAGGAGCTTTACTTGGATTTTGGATGGTTTTCAACCTTGGGTTCACATGTGCACTGAGTTATTCAAAGTCTCCAGGCATGGTGTTGCCTTTTGAGCCCATAACTATATCATTTGAGAAACTGCAATACTACATTGATACACCCCAGAAACTGCGAGACCAAGGTTTACCACCAAATCGGCTACAGCTTCTTCAGAACATAACCGGAGCATTTAGACCTGGCGTTCTTACAGCACTGATGGGCGTTAGTGGAGCGGGAAAGACCACGCTGATGGATGTTCTTTCAGGCAGGAAAACAGGAGGAATTATCGAAGGCGACATCAGAATTGGAGGACACCCAAAAGTGCAACAAACATATGCCAGAATATCTGCGTATTGCGAGCAAACTGATATTCATTCTCCAGAGCTGACGGTAGAAGAATCTATAGCATACTCAGCTTGGTTACGCTTGCCAGCCCAGATTGCCAGAAATGCTAGAGCTGAGTTTGTGAAGGAAGTACTTCAAATGATAGAGCTTGATGAAATCAAAGACGAGTTAGTTGGCATACCAGGTGGTAGTGGTATATCGACTGAACAGCGTAAAAGGCTAACAATTGCAGTAGAGCTAGTTTCGAATCCAACCATAATATTCATGGACGAACCCACATCCGGTTTAGATGCCCGAGCAGCTGCAATAGTTATGCGAGTTGTGAAAAAAATTGTCAGTACAGGGAGGACTGTTGTATGCACTATTCATCAGCCAAGTATTGACATATTTGAAGCATTCGATGAGCTTCTTTTGATGAAAAGAGGAGGACAAGTAATTTACTGTGGAGAACTGGGTCAAGATTCAAGTAAGCTTATCCAATACTTTGAGGGGATTTCTGGGGTTCCAAAAATCAAAGATAATTACAACCCAGCAACATGGATGCTAGAAGTCACAAGTCCTTCTGAAGAAGCCAAACTTGCCTTAGATTTTTCTGAGCTTTATAGAGAATCACATCTATGCCAACAGATCTTCAGCTATTATCTTCAAAAGCTTTCAAAAATTCTAGCAGTGCTACAATTTGCAAGTGATTCTGATATCAGGAAGAACAATGACCTAGTAAGAGAACACAGCTTTCCAGCACAAGGCTCAAAGGGATTGGATTTTCCTACTCGTTTCCCACGAAATGGGTGGGAGCAATTTAAAGCATGTCTGTGGAAGCGACATCTCTCCTACTGGAGAAATCCAAAATATAACCTCGGGCGGTTGTTAATCACTGCTGCATCCTCTATGATATTTGGAGCACTTCTTTGGCAGAAAGGACAGAAAATAAATGGTGAACAGGAGTTCTTCAGCATTCTAGGATCAATATTTATTTTACAACAAAACATGGGAATTGGTAACTGTTCCTCTGTTTTACCATTTGTTGCTTCTGAGCGCAATGTTGTATACCGAGAAAGGTTTGCTGGAATGTACTCCTCATGGGCATATTCTTTATCACAGGTGGTCATTGAACTTCCCTATGTATTCGTTCAAGCAGTTTTGTTTTCGACCATTACATATCCAACCATTGGTTTCGACTGGTCACTTTACAAAGTATTCTGGTATGTGTATGCTATGTTTTGTACGCTGCTCGACTACACTTACTTCGGTATGTTGGTTGCTGCTGTGACCCCAACTTTCCAAGTAGCTTCAGTATTAGCAAGTTGCTGCTACACCTTGTTGAATCTGTTCTCCGGATACCTAATACCTCAACCAAACATTCCTAAATGGTGGGTTTGGGGCTACTGGATTTGCCCTTTATCGTGGTCCCTAAAAGGTACTCTCACTTCACAGTATGGGGATTTAAACGAAGAAATAGTACTCGGCAGGGAGGAAAAGACTATCAGTGCCTTCCTAGGAAGCATTTATGGATATAACTACGATGATCTAGGAATTGTAGGAATTGTTCTTCTTGCATATCCACTAGTTTTAGCTTTTGCGTTCGCCATTGCGACAGAAAAACTGAACTTTCAGAGGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1353

Amino Acids

152.92

Weight (kDa)

8.4

Isoelectric Point (pI)

38.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 107 - 288 1.9e-17 ABC transporter
ABC2_membrane PF01061 445 - 656 1.4e-41 ABC-2 type transporter
PDR_assoc PF08370 661 - 717 3.2e-18 Plant PDR ABC transporter associated
ABC_tran PF00005 755 - 907 4.9e-20 ABC transporter
ABC2_membrane PF01061 1078 - 1291 3.9e-55 ABC-2 type transporter
ABC2_membrane_3 PF12698 1127 - 1323 2.9e-06 ABC-2 family transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000472)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15230 AT4G15230 AT4G15230 AT4G15230 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15236
fragaria_vesca FvH4_4g21360 FvH4_4g21360 FvH4_4g21390 FvH4_6g49431 FvH4_6g49450
malus_domestica MD09G1044300.v1.1 MD16G1137900.v1.1 MD16G1138000.v1.1
prunus_persica Prupe.1G106800_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.3G273300_v2.0.a1
pyrus_communis pycom111g03460 pycom16g12210
rosa_chinensis RchiOBHm_Chr2g0169331 RchiOBHm_Chr2g0169361 RchiOBHm_Chr2g0169371 RchiOBHm_Chr2g0169381 RchiOBHm_Chr2g0169411 RchiOBHm_Chr4g0427141 RchiOBHm_Chr4g0427321
rosa_laevigata RLG00000007252 RLG00000007258 RLG00000013974 RLG00000021856 RLG00000021857 RLG00000021858 RLG00000021859
rosa_multiflora Rmu_sc0000648.1_g000007 Rmu_sc0000648.1_g000009 Rmu_sc0003813.1_g000016 Rmu_sc0009165.1_g000008 Rmu_sc0022554.1_g000001
rosa_roxburghii Rroxscaffold_2G00082200 Rroxscaffold_2G00082230 Rroxscaffold_5G00368960 Rroxscaffold_5G00369010 Rroxscaffold_7G00200390
rosa_rugosa Rorug02G0539500 Rorug02G0539600 Rorug02G0539700 Rorug04G0216400 Rorug04G0216500 Rorug04G0216500 Rorug04G0216600
rosa_samantha Rh2AG610800 Rh2AG611000 Rh2AG611100 Rh2CG592800 Rh2CG593000 Rh2CG593200 Rh2DG634900 Rh2DG635100 Rh2DG635200 Rh2DG635400 Rh4AG272200 Rh4AG272900 Rh4DG275600 Rh4DG276000
rosa_wichuraiana Rw2G050640 Rw2G050660 Rw2G050670 Rw2G050680 Rw4G023610 Rw4G023680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1903
Acc65I GGTACC 1 cut(s) 1161
AccB1I GGYRCC 1 cut(s) 1161
AccB7I CCANNNNNTGG 3 cut(s) 383, 3224, 3608
AccBSI CCGCTC 1 cut(s) 2327
AccI GTMKAC 3 cut(s) 1029, 1467, 3486
AccIII TCCGGA 1 cut(s) 3773
AciI CCGC 2 cut(s) 2327, 3301
AclWI GGATC 4 cut(s) 724, 1152, 1831, 3409
AcsI RAATTY 4 cut(s) 315, 786, 1964, 3099
AcuI CTGAAG 9 cut(s) 947, 1661, 1714, 2032, 2249, 3024, 3055, 3373, 3714
AfiI CCNNNNNNNGG 9 cut(s) 383, 739, 1097, 1120, 1612, 2720, 3182, 3224, 3608
AflIII ACRYGT 1 cut(s) 2120
AhdI GACNNNNNGTC 1 cut(s) 1571
AhlI ACTAGT 2 cut(s) 1426, 4003
AjnI CCWGG 5 cut(s) 376, 576, 2146, 2290, 2620
AjuI GAANNNNNNNTTGG 2 cut(s) 816, 848
Alw21I GWGCWC 4 cut(s) 32, 52, 2128, 3343
Alw26I GTCTC 5 cut(s) 300, 971, 1138, 2148, 2223
Alw44I GTGCAC 1 cut(s) 2124
AlwI GGATC 4 cut(s) 724, 1152, 1831, 3409
AlwNI CAGNNNCTG 2 cut(s) 1826, 2224
Ama87I CYCGRG 2 cut(s) 2733, 3296
Aor13HI TCCGGA 1 cut(s) 3773
AoxI GGCC 5 cut(s) 675, 835, 1037, 1932, 2014
ApaLI GTGCAC 1 cut(s) 2124
ApeKI GCWGC 8 cut(s) 916, 1631, 2738, 2741, 3317, 3667, 3701, 3747
ApoI RAATTY 4 cut(s) 315, 786, 1964, 3099
Asp700I GAANNNNTTC 5 cut(s) 213, 1362, 1810, 2571, 3767
Asp718I GGTACC 1 cut(s) 1161
AspA2I CCTAGG 1 cut(s) 3934
AspLEI GCGC 1 cut(s) 3477
AspS9I GGNCC 4 cut(s) 371, 836, 1037, 3846
AsuHPI GGTGA 2 cut(s) 833, 3386
AsuII TTCGAA 1 cut(s) 2684
AvaI CYCGRG 2 cut(s) 2733, 3296
AvaII GGWCC 2 cut(s) 371, 3846
AvrII CCTAGG 1 cut(s) 3934
BaeGI GKGCMC 1 cut(s) 2128
BaeI ACNNNNGTAYC 2 cut(s) 747, 780
BanI GGYRCC 1 cut(s) 1161
BanII GRGCYC 2 cut(s) 52, 2172
BarI GAAGNNNNNNTAC 4 cut(s) 3850, 3882, 3882, 3914
BauI CACGAG 1 cut(s) 1754
BbsI GAAGAC 2 cut(s) 500, 1567
Bbv12I GWGCWC 4 cut(s) 32, 52, 2128, 3343
BbvI GCAGC 8 cut(s) 928, 1618, 2728, 2750, 3304, 3654, 3688, 3734
BccI CCATC 5 cut(s) 1003, 1643, 2092, 2305, 2338
BceAI ACGGC 1 cut(s) 74
BcgI CGANNNNNNTGC 2 cut(s) 1460, 1494
BciT130I CCWGG 5 cut(s) 378, 578, 2148, 2292, 2622
BciVI GTATCC 1 cut(s) 3770
BcoDI GTCTC 5 cut(s) 300, 971, 1138, 2148, 2223
BcuI ACTAGT 2 cut(s) 1426, 4003
BfmI CTRYAG 5 cut(s) 9, 914, 2010, 2255, 2496
BfuAI ACCTGC 1 cut(s) 1903
BfuI GTATCC 1 cut(s) 3770
BglII AGATCT 2 cut(s) 1473, 3066
BisI GCNGC 8 cut(s) 917, 1632, 2739, 2742, 3318, 3668, 3702, 3748
BlnI CCTAGG 1 cut(s) 3934
BlsI GCNGC 8 cut(s) 918, 1633, 2740, 2743, 3319, 3669, 3703, 3749
BmcAI AGTACT 4 cut(s) 1157, 1487, 2571, 3900
Bme1390I CCNGG 5 cut(s) 378, 578, 2148, 2292, 2622
Bme18I GGWCC 2 cut(s) 371, 3846
BmeRI GACNNNNNGTC 1 cut(s) 1571
BmeT110I CYCGRG 2 cut(s) 2733, 3296
BmgT120I GGNCC 4 cut(s) 371, 836, 1037, 3846
BmiI GGNNCC 3 cut(s) 1163, 2943, 3848
BmrFI CCNGG 5 cut(s) 378, 578, 2148, 2292, 2622
BmrI ACTGGG 1 cut(s) 2900
BmsI GCATC 8 cut(s) 345, 760, 1654, 1768, 1802, 2719, 2970, 3329
BmuI ACTGGG 1 cut(s) 2900
BpiI GAAGAC 2 cut(s) 500, 1567
BpmI CTGGAG 4 cut(s) 360, 2130, 2457, 3295
Bpu10I CCTNAGC 1 cut(s) 51
Bpu14I TTCGAA 1 cut(s) 2684
BpuEI CTTGAG 3 cut(s) 282, 419, 1546
BsaAI YACGTR 2 cut(s) 931, 1451
BsaI GGTCTC 3 cut(s) 971, 1138, 2223
BsaWI WCCGGW 3 cut(s) 2276, 2720, 3773
BsaXI ACNNNNNCTCC 2 cut(s) 2856, 2886
Bsc4I CCNNNNNNNGG 9 cut(s) 383, 739, 1097, 1120, 1612, 2720, 3182, 3224, 3608
Bse1I ACTGG 6 cut(s) 561, 1163, 2895, 3278, 3623, 3830
Bse3DI GCAATG 6 cut(s) 780, 1419, 1537, 1655, 3484, 4026
BseAI TCCGGA 1 cut(s) 3773
BseBI CCWGG 5 cut(s) 378, 578, 2148, 2292, 2622
BseLI CCNNNNNNNGG 9 cut(s) 383, 739, 1097, 1120, 1612, 2720, 3182, 3224, 3608
BseMI GCAATG 6 cut(s) 780, 1419, 1537, 1655, 3484, 4026
BseMII CTCAG 8 cut(s) 65, 503, 525, 2120, 2520, 2544, 3024, 3462
BseNI ACTGG 6 cut(s) 561, 1163, 2895, 3278, 3623, 3830
BseRI GAGGAG 4 cut(s) 111, 1265, 2877, 3505
BseSI GKGCMC 1 cut(s) 2128
BseXI GCAGC 8 cut(s) 928, 1618, 2728, 2750, 3304, 3654, 3688, 3734
BseYI CCCAGC 2 cut(s) 838, 2968
BshFI GGCC 5 cut(s) 677, 837, 1039, 1934, 2016
BshNI GGYRCC 1 cut(s) 1161
BsiHKAI GWGCWC 4 cut(s) 32, 52, 2128, 3343
BsiHKCI CYCGRG 2 cut(s) 2733, 3296
BsiSI CCGG 4 cut(s) 1628, 2277, 2721, 3774
BslFI GGGAC 3 cut(s) 162, 629, 3832
BslI CCNNNNNNNGG 9 cut(s) 383, 739, 1097, 1120, 1612, 2720, 3182, 3224, 3608
BsmAI GTCTC 5 cut(s) 300, 971, 1138, 2148, 2223
BsmFI GGGAC 3 cut(s) 162, 629, 3832
BsmI GAATGC 2 cut(s) 2834, 3393
BsnI GGCC 5 cut(s) 677, 837, 1039, 1934, 2016
Bso31I GGTCTC 3 cut(s) 971, 1138, 2223
BsoBI CYCGRG 2 cut(s) 2733, 3296
Bsp119I TTCGAA 1 cut(s) 2684
Bsp1286I GDGCHC 5 cut(s) 32, 52, 2128, 2172, 3343
Bsp13I TCCGGA 1 cut(s) 3773
Bsp143I GATC 9 cut(s) 249, 729, 1144, 1473, 1788, 1823, 3066, 3401, 3964
Bsp19I CCATGG 1 cut(s) 1033
BspACI CCGC 2 cut(s) 2327, 3301
BspANI GGCC 5 cut(s) 677, 837, 1039, 1934, 2016
BspCNI CTCAG 8 cut(s) 64, 502, 526, 2121, 2519, 2545, 3025, 3463
BspEI TCCGGA 1 cut(s) 3773
BspLI GGNNCC 3 cut(s) 1163, 2943, 3848
BspMAI CTGCAG 1 cut(s) 918
BspMI ACCTGC 1 cut(s) 1903
BspPI GGATC 4 cut(s) 724, 1152, 1831, 3409
BspT104I TTCGAA 1 cut(s) 2684
BspT107I GGYRCC 1 cut(s) 1161
BspTNI GGTCTC 3 cut(s) 971, 1138, 2223
BsrBI CCGCTC 1 cut(s) 2327
BsrDI GCAATG 6 cut(s) 780, 1419, 1537, 1655, 3484, 4026
BsrI ACTGG 6 cut(s) 561, 1163, 2895, 3278, 3623, 3830
BssMI GATC 9 cut(s) 249, 729, 1144, 1473, 1788, 1823, 3066, 3401, 3964
BssNAI GTATAC 2 cut(s) 1030, 3487
BssSI CACGAG 1 cut(s) 1754
BssT1I CCWWGG 7 cut(s) 733, 1033, 1797, 1976, 2110, 2233, 3934
Bst1107I GTATAC 2 cut(s) 1030, 3487
Bst2BI CACGAG 1 cut(s) 1754
Bst2UI CCWGG 5 cut(s) 378, 578, 2148, 2292, 2622
Bst6I CTCTTC 1 cut(s) 1242
BstBAI YACGTR 2 cut(s) 931, 1451
BstBI TTCGAA 1 cut(s) 2684
BstC8I GCNNGC 6 cut(s) 1345, 2018, 2022, 2453, 2522, 2526
BstDSI CCRYGG 2 cut(s) 1033, 2035
BstENI CCTNNNNNAGG 1 cut(s) 1118
BstHHI GCGC 1 cut(s) 3477
BstKTI GATC 9 cut(s) 252, 732, 1147, 1476, 1791, 1826, 3069, 3404, 3967
BstMAI GTCTC 5 cut(s) 300, 971, 1138, 2148, 2223
BstMBI GATC 9 cut(s) 249, 729, 1144, 1473, 1788, 1823, 3066, 3401, 3964
BstNI CCWGG 5 cut(s) 378, 578, 2148, 2292, 2622
BstNSI RCATGY 3 cut(s) 1347, 2124, 3249
BstSCI CCNGG 5 cut(s) 376, 576, 2146, 2290, 2620
BstSFI CTRYAG 5 cut(s) 9, 914, 2010, 2255, 2496
BstSLI GKGCMC 1 cut(s) 2128
BstSNI TACGTA 1 cut(s) 1451
BstV1I GCAGC 8 cut(s) 928, 1618, 2728, 2750, 3304, 3654, 3688, 3734
BstV2I GAAGAC 2 cut(s) 500, 1567
BstX2I RGATCY 4 cut(s) 729, 1473, 1823, 3066
BstXI CCANNNNNNTGG 4 cut(s) 584, 949, 2344, 2976
BstYI RGATCY 4 cut(s) 729, 1473, 1823, 3066
BstZ17I GTATAC 2 cut(s) 1030, 3487
BsuI GTATCC 1 cut(s) 3770
BsuRI GGCC 5 cut(s) 677, 837, 1039, 1934, 2016
BtgI CCRYGG 2 cut(s) 1033, 2035
BtsI GCAGTG 3 cut(s) 388, 3114, 3312
BveI ACCTGC 1 cut(s) 1903
Cac8I GCNNGC 6 cut(s) 1345, 2018, 2022, 2453, 2522, 2526
CaiI CAGNNNCTG 2 cut(s) 1826, 2224
CfoI GCGC 1 cut(s) 3477
Cfr13I GGNCC 4 cut(s) 371, 836, 1037, 3846
CsiI ACCWGGT 1 cut(s) 2620
DpnI GATC 9 cut(s) 251, 731, 1146, 1475, 1790, 1825, 3068, 3403, 3966
DpnII GATC 9 cut(s) 249, 729, 1144, 1473, 1788, 1823, 3066, 3401, 3964
DraI TTTAAA 2 cut(s) 3241, 3885
DriI GACNNNNNGTC 1 cut(s) 1571
Eam1104I CTCTTC 1 cut(s) 1242
Eam1105I GACNNNNNGTC 1 cut(s) 1571
EarI CTCTTC 1 cut(s) 1242
Ecl136II GAGCTC 1 cut(s) 50
Eco105I TACGTA 1 cut(s) 1451
Eco130I CCWWGG 7 cut(s) 733, 1033, 1797, 1976, 2110, 2233, 3934
Eco24I GRGCYC 2 cut(s) 52, 2172
Eco31I GGTCTC 3 cut(s) 971, 1138, 2223
Eco32I GATATC 4 cut(s) 867, 1548, 2070, 3136
Eco47I GGWCC 2 cut(s) 371, 3846
Eco53kI GAGCTC 1 cut(s) 50
Eco57I CTGAAG 9 cut(s) 947, 1661, 1714, 2032, 2249, 3024, 3055, 3373, 3714
Eco88I CYCGRG 2 cut(s) 2733, 3296
EcoICRI GAGCTC 1 cut(s) 50
EcoNI CCTNNNNNAGG 1 cut(s) 1118
EcoRII CCWGG 5 cut(s) 376, 576, 2146, 2290, 2620
EcoRV GATATC 4 cut(s) 867, 1548, 2070, 3136
EcoT14I CCWWGG 7 cut(s) 733, 1033, 1797, 1976, 2110, 2233, 3934
EcoT22I ATGCAT 1 cut(s) 1669
EcoT38I GRGCYC 2 cut(s) 52, 2172
ErhI CCWWGG 7 cut(s) 733, 1033, 1797, 1976, 2110, 2233, 3934
FalI AAGNNNNNCTT 8 cut(s) 189, 221, 1239, 1271, 1332, 1364, 3000, 3032
FaqI GGGAC 3 cut(s) 162, 629, 3832
FauI CCCGC 1 cut(s) 2320
FauNDI CATATG 1 cut(s) 2428
FblI GTMKAC 3 cut(s) 1029, 1467, 3486
Fnu4HI GCNGC 8 cut(s) 917, 1632, 2739, 2742, 3318, 3668, 3702, 3748
FriOI GRGCYC 2 cut(s) 52, 2172
Fsp4HI GCNGC 8 cut(s) 917, 1632, 2739, 2742, 3318, 3668, 3702, 3748
GlaI GCGC 1 cut(s) 3476
GluI GCNGC 8 cut(s) 917, 1632, 2739, 2742, 3318, 3668, 3702, 3748
GsaI CCCAGC 2 cut(s) 842, 2972
GsuI CTGGAG 4 cut(s) 360, 2130, 2457, 3295
HaeIII GGCC 5 cut(s) 677, 837, 1039, 1934, 2016
HapII CCGG 4 cut(s) 1628, 2277, 2721, 3774
HhaI GCGC 1 cut(s) 3477
Hin6I GCGC 1 cut(s) 3475
HinP1I GCGC 1 cut(s) 3475
HincII GTYRAC 2 cut(s) 515, 1468
HindII GTYRAC 2 cut(s) 515, 1468
HindIII AAGCTT 4 cut(s) 256, 1241, 2909, 3089
HpaII CCGG 4 cut(s) 1628, 2277, 2721, 3774
HphI GGTGA 2 cut(s) 833, 3386
Hpy166II GTNNAC 8 cut(s) 515, 1030, 1468, 2118, 2126, 2240, 3377, 3487
Hpy8I GTNNAC 8 cut(s) 515, 1030, 1468, 2118, 2126, 2240, 3377, 3487
Hpy99I CGWCG 1 cut(s) 1472
HpyAV CCTTC 8 cut(s) 357, 667, 1010, 1821, 2378, 2557, 3009, 3940
HpyCH4IV ACGT 2 cut(s) 930, 1450
HpySE526I ACGT 2 cut(s) 930, 1450
HspAI GCGC 1 cut(s) 3475
Kpn2I TCCGGA 1 cut(s) 3773
KpnI GGTACC 1 cut(s) 1165
Kzo9I GATC 9 cut(s) 249, 729, 1144, 1473, 1788, 1823, 3066, 3401, 3964
LmnI GCTCC 9 cut(s) 47, 890, 1252, 1978, 2078, 2279, 2324, 3232, 3338
Lsp1109I GCAGC 8 cut(s) 928, 1618, 2728, 2750, 3304, 3654, 3688, 3734
LweI GCATC 8 cut(s) 345, 760, 1654, 1768, 1802, 2719, 2970, 3329
MabI ACCWGGT 1 cut(s) 2620
MaeII ACGT 2 cut(s) 930, 1450
MalI GATC 9 cut(s) 251, 731, 1146, 1475, 1790, 1825, 3068, 3403, 3966
MbiI CCGCTC 1 cut(s) 2327
MboI GATC 9 cut(s) 249, 729, 1144, 1473, 1788, 1823, 3066, 3401, 3964
MfeI CAATTG 5 cut(s) 684, 745, 759, 920, 2664
MflI RGATCY 4 cut(s) 729, 1473, 1823, 3066
MhlI GDGCHC 5 cut(s) 32, 52, 2128, 2172, 3343
MlyI GAGTC 2 cut(s) 352, 919
MmeI TCCRAC 3 cut(s) 1213, 2714, 3626
Mph1103I ATGCAT 1 cut(s) 1669
MroI TCCGGA 1 cut(s) 3773
MroXI GAANNNNTTC 5 cut(s) 213, 1362, 1810, 2571, 3767
MseI TTAA 8 cut(s) 68, 116, 1218, 1844, 2031, 3240, 3308, 3884
MslI CAYNNNNRTG 6 cut(s) 668, 1185, 1290, 2342, 3055, 3250
MspA1I CMGCKG 1 cut(s) 2741
MspI CCGG 4 cut(s) 1628, 2277, 2721, 3774
MspR9I CCNGG 5 cut(s) 378, 578, 2148, 2292, 2622
MunI CAATTG 5 cut(s) 684, 745, 759, 920, 2664
Mva1269I GAATGC 2 cut(s) 2834, 3393
MvaI CCWGG 5 cut(s) 378, 578, 2148, 2292, 2622
NcoI CCATGG 1 cut(s) 1033
NdeI CATATG 1 cut(s) 2428
NdeII GATC 9 cut(s) 249, 729, 1144, 1473, 1788, 1823, 3066, 3401, 3964
NlaIV GGNNCC 3 cut(s) 1163, 2943, 3848
NmeAIII GCCGAG 1 cut(s) 3882
NmuCI GTSAC 4 cut(s) 268, 2989, 3621, 3706
NsiI ATGCAT 1 cut(s) 1669
NspI RCATGY 3 cut(s) 1347, 2124, 3249
NspV TTCGAA 1 cut(s) 2684
PaeI GCATGC 1 cut(s) 1347
PciI ACATGT 1 cut(s) 2120
PcsI WCGNNNNNNNCGW 1 cut(s) 3217
PctI GAATGC 2 cut(s) 2834, 3393
PdmI GAANNNNTTC 5 cut(s) 213, 1362, 1810, 2571, 3767
PfeI GAWTC 8 cut(s) 938, 1645, 2492, 2686, 2900, 3047, 3128, 3763
PflMI CCANNNNNTGG 3 cut(s) 383, 3224, 3608
PkrI GCNGC 8 cut(s) 918, 1633, 2740, 2743, 3319, 3669, 3703, 3749
PleI GAGTC 2 cut(s) 352, 919
PpsI GAGTC 2 cut(s) 352, 919
Ppu21I YACGTR 2 cut(s) 931, 1451
PscI ACATGT 1 cut(s) 2120
Psp124BI GAGCTC 1 cut(s) 52
Psp6I CCWGG 5 cut(s) 376, 576, 2146, 2290, 2620
PspFI CCCAGC 2 cut(s) 838, 2968
PspGI CCWGG 5 cut(s) 376, 576, 2146, 2290, 2620
PspN4I GGNNCC 3 cut(s) 1163, 2943, 3848
PspPI GGNCC 4 cut(s) 371, 836, 1037, 3846
PsrI GAACNNNNNNTAC 2 cut(s) 3969, 4001
PstI CTGCAG 1 cut(s) 918
PstNI CAGNNNCTG 2 cut(s) 1826, 2224
PsuI RGATCY 4 cut(s) 729, 1473, 1823, 3066
PvuII CAGCTG 1 cut(s) 2741
RseI CAYNNNNRTG 6 cut(s) 668, 1185, 1290, 2342, 3055, 3250
SacI GAGCTC 1 cut(s) 52
SalI GTCGAC 1 cut(s) 1466
SaqAI TTAA 8 cut(s) 68, 116, 1218, 1844, 2031, 3240, 3308, 3884
SatI GCNGC 8 cut(s) 917, 1632, 2739, 2742, 3318, 3668, 3702, 3748
Sau3AI GATC 9 cut(s) 249, 729, 1144, 1473, 1788, 1823, 3066, 3401, 3964
Sau96I GGNCC 4 cut(s) 371, 836, 1037, 3846
ScaI AGTACT 4 cut(s) 1157, 1487, 2571, 3900
SchI GAGTC 2 cut(s) 352, 919
ScrFI CCNGG 5 cut(s) 378, 578, 2148, 2292, 2622
SduI GDGCHC 5 cut(s) 32, 52, 2128, 2172, 3343
SexAI ACCWGGT 1 cut(s) 2620
SfaNI GCATC 8 cut(s) 345, 760, 1654, 1768, 1802, 2719, 2970, 3329
SfcI CTRYAG 5 cut(s) 9, 914, 2010, 2255, 2496
SfuI TTCGAA 1 cut(s) 2684
SinI GGWCC 2 cut(s) 371, 3846
SmiMI CAYNNNNRTG 6 cut(s) 668, 1185, 1290, 2342, 3055, 3250
SmlI CTYRAG 3 cut(s) 297, 434, 1561
SmoI CTYRAG 3 cut(s) 297, 434, 1561
SnaBI TACGTA 1 cut(s) 1451
SpeI ACTAGT 2 cut(s) 1426, 4003
SphI GCATGC 1 cut(s) 1347
SsiI CCGC 2 cut(s) 2327, 3301
SspI AATATT 2 cut(s) 2700, 3408
SstI GAGCTC 1 cut(s) 52
StyD4I CCNGG 5 cut(s) 376, 576, 2146, 2290, 2620
StyI CCWWGG 7 cut(s) 733, 1033, 1797, 1976, 2110, 2233, 3934
TaiI ACGT 2 cut(s) 933, 1453
TaqI TCGA 9 cut(s) 1269, 1467, 2382, 2639, 2684, 2838, 3587, 3615, 3672
TatI WGTACW 6 cut(s) 1155, 1485, 2569, 2777, 3510, 3898
TfiI GAWTC 8 cut(s) 938, 1645, 2492, 2686, 2900, 3047, 3128, 3763
Tru1I TTAA 8 cut(s) 68, 116, 1218, 1844, 2031, 3240, 3308, 3884
Tru9I TTAA 8 cut(s) 68, 116, 1218, 1844, 2031, 3240, 3308, 3884
TseFI GTSAC 4 cut(s) 268, 2989, 3621, 3706
TseI GCWGC 8 cut(s) 916, 1631, 2738, 2741, 3317, 3667, 3701, 3747
Tsp45I GTSAC 4 cut(s) 268, 2989, 3621, 3706
TspGWI ACGGA 3 cut(s) 292, 1070, 1638
Van91I CCANNNNNTGG 3 cut(s) 383, 3224, 3608
VneI GTGCAC 1 cut(s) 2124
VpaK11BI GGWCC 2 cut(s) 371, 3846
XagI CCTNNNNNAGG 1 cut(s) 1118
XapI RAATTY 4 cut(s) 315, 786, 1964, 3099
XbaI TCTAGA 1 cut(s) 1352
XceI RCATGY 3 cut(s) 1347, 2124, 3249
XcmI CCANNNNNNNNNTGG 2 cut(s) 2701, 3225
XmaJI CCTAGG 1 cut(s) 3934
XmiI GTMKAC 3 cut(s) 1029, 1467, 3486
XmnI GAANNNNTTC 5 cut(s) 213, 1362, 1810, 2571, 3767
ZrmI AGTACT 4 cut(s) 1157, 1487, 2571, 3900
Zsp2I ATGCAT 1 cut(s) 1669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.