Rw2G050680

pleiotropic drug resistance protein 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000472
Physical Location & Seq
Forward (+)
78309282 .. 78320470
11189 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G050680.1

Sequence Viewer

Length: 4242 bp
ATGGACTTGGCTGCAGAGATAGGAAGAAGTGTCAGGACCTCGTTTCGTGAGCATGCATCGAGTTTCAGGAGCACTGCAGATGTAAGGTCCATAAAGAAGAATGAAGACGATGAAGTAGAGCTGCAGTGGGCCGCCATTGAGAGGTTGCCAACGTTTGAGCGCATTAGGACTTCATTGTTTGATTGCAGCAAAGATGAGAAGGAAGATGAGGCTAGTAACAAGAGTAAGATGGCACAGATTGATGTGTCGAAGCTAGGATCAATGGAGAGGCATGTGGTCATTCAGAAGTTGATCAAGGAAATTGAGGGAGATAACCACCGGCTCTTGCAGAAACTCAGAGAAAGAATAGACAGAGTAGGACTGCAACTGCCGACTGTGGAAGTTCGATACCAGAATTTGTTAGTAGAAGCAGAATATGAAGTAGTTCAAGGAAAACCTCTCCCTACACTATGGAACACCGTTAAAAGCTTATTCTCTGTTATGACTCAGATAGTTGGGTGCAAGCCTCAAGCATACAAGCTAGAAATTCTCAGAGATATCAGTGGCATCATCAAACCATCAAGGATGACTCTATTGCTTGGCCCTCCAGGCTGTGGGAAATCCACTTTATTACAGGCGCTTGCAGGGAAACTTAACCATTCTCTCAAGGTCCGGGGAAATGTAACCTACAATGGTTATAAGCTCAATGAGTTTGTTCCACAGAAGACATCAGCTTATATAAGTCAATATGACTTGCACATTTCTGAAATGACTGTGAGGGAAGCACTGGATTTCGCAGCACGCTGTCAGGGTATTGGAAGCAGAGCAGATATAATGAAAGAAGTCACCAGAAGGGAGAAGCAAGAAGGAATTGTCCCAGATTCAGATATTGATACTTATATGAAGGCCATTTCGGTAGAAGGGTTGAAAAAATCTCTCATGACAGACTACATAATAAAGATCCTTGGGCTAGACATGTGTGCTGAGACAATTGTAGGAGATCCAATGCAAAGAGGAATTTCGGGTGGCCAAAAGAAAAGACTAACAACAGGGGAAATGATGATTGGTCCAGCAAGAGCTTTCTTTATGGACGAGATATCAACAGGCTTAGACAGTTCCACCACCTTCCAAATCGTTACTTGTCTTCAGCAATTGACACACCTAACAGAATCCACCATCTTGGTTTCACTTCTTCAGCCAACACCTGAGACCTTTCATCTGTTTGATGACATTATCTTGATGGCAGAAGGAAAAGTTGTATACCATGGGCCTAGTGATAATGTGGTAGAGTTCTTTGAGAACTGTGGTTTCCGTAGCCCACCACGAAAAGGCATTTCTGACTTCCTCCAAGAGGTGGTTTCCCAAAAGGATCAAGCAAAGTACTGGTACGATGAAGACCAACCCCATAGTTATGTTACTGTCGACAACTTTGTAGATATGTTCAAGGACTTCCATGTTGGAAAGAAGCTCGATGAGGAACTTCAGGAGCCTTTCAATCGATCCGAGTTCCATAAAGATGCTTTGTCATTTAACATGTACTCATTGACAAAATGGGAACTCTTCAAAGCATGCCTTGCTAGAGAATGGCTTCTGATGAAGCGCAACTCGTTTGTCCATGTATTCAAATCAGCACAGCTTGTGGTCATTGCGCTAGTAACAATGACAGTGTTTCTTCGTACGTGGATGAAAGTCGATGAGATCCATTCAAATTTCTTTATGAGTTCTTTGTTCTATGCTCTACTTAGATTGGTGTGCAATGGCACTGCAGAGTTATCATTGACTGCTTCCAGACTTGCAGTGTTTTACAAGCAAAGAGACTTTTATTTCTATCCTGCGTGGGCTTATTCAGTTCCATCTGCCATCCTAAAGATTCCATTTTCATTGATAGACGCTTTTCTATGGACAGCACTTACTTATTATGTCATCGGTTACAGTCCTGAACCCGAGAGGTTCTTCAAGCAATTCATTCTTTTATTCCTTGTCCATCAAGTAGCAATATCACTGTTCCGTTTGATTGCATCCTTGGTCCGAAATCCTTCTCTTGCAGCCCCTGTTGGTCTTTTCTCCTTAATTGTAATGTTTCTATTTGGCGGCTTCATAATTCCAAAAACTTCTCTACCTAGCTGGCTGGAATGGGGCTTTTGGCTTTCCCCATTGGTATATGCAGAAATTGGTGCTACAGGAAACGAATTCCATGCTCCAAGGTGGCAAAAGCTCTCATCTTCAAATATCACAATAGGTCAGCAAGCTCTAAAAGACAGTGGTCTTGATTTCAGTGAATACTTCTATTGGATATCTATAGGAGCTTTACTTGGATTTTGGTTGGTTTTCAACCTAGGTTTCACCTGTGCTCTCAGTTATTTAAAGTCTCCAGGGAGTTCTCGAACAATCATTTCTCATGAAAGGTTGTCCCGTCTGAAGGAAAAGGAAAGTCTAAACAATTCCCCTCGAAAAAAAAAATTAACTCGTATAGAAACTCGAATAAGAGTTGAAGATTCTACAAATATAGGGGTCTTACCTTTTGAACCCTTGACTATATCATTTGAGAAGGTGCAATATTTTGTCGACTTACCCCAGAAACTGAGAGAGCAAGGTGTTCCACCAAAACGACTACAACTTCTTCAAGATATAACTGGTGCATTTAGACCTGGAGTTCTTACAGCACTTATGGGTGTTAGTGGAGCTGGAAAGACAACACTGATGGATGTTCTTTCAGGAAGGAAAACTGGTGGAATTATTGAAGGAGACATTAGAATTGGAGGACACCCAAAAGTCCAAGAAACATATGCCAGAATATCTGGGTATTGCGAGCAAAGTGACATACATTCCCCACAGATAACAGTAGAAGAGTCGGTAACATACTCAGCTTGGTTACGTTTGCCAGCACAGGTTGATAGAACAGTCAGATCTGAATTTGTGAGAGAAGTTCTACAAATGATCGAGCTTGATGAAATCAAGGACGAACTAGTGGGCGTACCTGGTAGGGGTATATCGACTGAACAGCGCAAACGGCTTACTATAGCAGTGGAGCTTGTTTCAAATCCATCCATAATGTTCATGGATGAACCCACCTCTGGTTTAGATGCCAGAGCAGCTGCAATAGTTATGCGAGTGGTCAAAAACATTGTCAGCACTAGGAGGACTGTTGTATGCACCATTCACCAGCCCAGTATTGACATCTTTGAAGCATTCGATGAGCTTATTTTAATGAAAATAGGAGGACAGCTAATCTACAGTGGAGCGCTGGGTCAAAATTCAAGCAAGCTTATTGAATACTTTGAGCGTATTCCTGGGGTTCCAAACATCAAAGAAAATTACAACCCCGCAACATGGATGTTAGAGGTCACCGGTCCTTCTGCAGAAGCTAAACTTGGCTTAGATTTTGCTCAACTCTATAGAGAATCACATCTATGCCGGGAGAACGATGACCTAGTTAGAGAACTCAGCTGTCCAGCAGAAGGGGCAAAGAAACTACATTTTTCTACTCGCTTTGCACAAAATGAATGGGAGCAATTTAAAGCCTGCCTCTGGAAGCAACATCTCTCCTACTGGAGAAATCCACAATATAACCTGGGCAGATTGACAATTACTGCTGCATCCTCTTTGTTATTCGGAGCACTTCTTTGGCAGAAAGGGCAGACAGTAGATAATGAACAGGATTTTTTCAACATTCTGGGATCAATGTTTGTTTTACTGCAATTCATGGGAATAGGAAACTGCTCCTCTGTTTTACCCTGTATTGCTTCAGAGCGGAATGTTGTATACCGAGAAAGGTTTGCTGGAATGTACTCCTCATGGGCCTATTCATTTGCGCAGGTGATCATTGAAATTCCATACATATTTCTTCAGGCAGTATTGTTTTTGGCAATCACATATCCAACCATAAATTTCCACTGGACACTATACCAAGTGTTTTGGTATGTCTATGCCATGTTTTGTACACTGCTCTACTTCAGTTACTTCGGCATATTGCTTGCAGCTTTGACCCCAACATTCCCAGTGGCTATGGTCCTAGCCAGTTTCTGTTACACCATGTTCAACTTATTCTCAGGTTTCCTCGTGCCTGGACCGAAAATTCCTAAATGGTGGGTTTGGGGTTATTGGATTTGCCCTGTAGCATGGTCCTTAAACGGTCTTCTCACTTCACAGTATGGAGATCAAAACAAGGTGATAGTAGTACATGGGGAGAAAAAGACCATCATTTTTAAAAAAAAGAAAAAATCAAGAGAAAATGCATTCAAAATTTCTTGGCATATTATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1413

Amino Acids

160.85

Weight (kDa)

8.32

Isoelectric Point (pI)

39.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 177 - 358 9.7e-15 ABC transporter
ABC2_membrane_7 PF19055 392 - 445 1e-05 ABC-2 type transporter
ABC2_membrane PF01061 513 - 724 3e-44 ABC-2 type transporter
PDR_assoc PF08370 729 - 791 1.4e-21 Plant PDR ABC transporter associated
ABC_tran PF00005 867 - 1018 3.3e-20 ABC transporter
ABC2_membrane PF01061 1163 - 1377 1.9e-58 ABC-2 type transporter
ABC2_membrane_3 PF12698 1212 - 1379 2.2e-06 ABC-2 family transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000472)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15230 AT4G15230 AT4G15230 AT4G15230 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15236
fragaria_vesca FvH4_4g21360 FvH4_4g21360 FvH4_4g21390 FvH4_6g49431 FvH4_6g49450
malus_domestica MD09G1044300.v1.1 MD16G1137900.v1.1 MD16G1138000.v1.1
prunus_persica Prupe.1G106800_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.3G273300_v2.0.a1
pyrus_communis pycom111g03460 pycom16g12210
rosa_chinensis RchiOBHm_Chr2g0169331 RchiOBHm_Chr2g0169361 RchiOBHm_Chr2g0169371 RchiOBHm_Chr2g0169381 RchiOBHm_Chr2g0169411 RchiOBHm_Chr4g0427141 RchiOBHm_Chr4g0427321
rosa_laevigata RLG00000007252 RLG00000007258 RLG00000013974 RLG00000021856 RLG00000021857 RLG00000021858 RLG00000021859
rosa_multiflora Rmu_sc0000648.1_g000007 Rmu_sc0000648.1_g000009 Rmu_sc0003813.1_g000016 Rmu_sc0009165.1_g000008 Rmu_sc0022554.1_g000001
rosa_roxburghii Rroxscaffold_2G00082200 Rroxscaffold_2G00082230 Rroxscaffold_5G00368960 Rroxscaffold_5G00369010 Rroxscaffold_7G00200390
rosa_rugosa Rorug02G0539500 Rorug02G0539600 Rorug02G0539700 Rorug04G0216400 Rorug04G0216500 Rorug04G0216500 Rorug04G0216600
rosa_samantha Rh2AG610800 Rh2AG611000 Rh2AG611100 Rh2CG592800 Rh2CG593000 Rh2CG593200 Rh2DG634900 Rh2DG635100 Rh2DG635200 Rh2DG635400 Rh4AG272200 Rh4AG272900 Rh4DG275600 Rh4DG276000
rosa_wichuraiana Rw2G050640 Rw2G050660 Rw2G050670 Rw2G050680 Rw4G023610 Rw4G023680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 678
AarI CACCTGC 1 cut(s) 3784
Acc16I TGCGCA 1 cut(s) 3792
Acc36I ACCTGC 1 cut(s) 3784
AccB7I CCANNNNNTGG 2 cut(s) 593, 1333
AccBSI CCGCTC 1 cut(s) 3730
AccI GTMKAC 4 cut(s) 1239, 1401, 2544, 3741
AciI CCGC 4 cut(s) 132, 2070, 3303, 3730
AclI AACGTT 1 cut(s) 152
AclWI GGATC 7 cut(s) 265, 934, 974, 1356, 1473, 1672, 3664
AcoI YGGCCR 1 cut(s) 1006
AcuI CTGAAG 7 cut(s) 1109, 1157, 1445, 2417, 3708, 3809, 3916
AfaI GTAC 8 cut(s) 1361, 1367, 1517, 1657, 2955, 3767, 3919, 4158
AfeI AGCGCT 1 cut(s) 3222
AflIII ACRYGT 2 cut(s) 954, 1512
AgeI ACCGGT 1 cut(s) 3326
AhlI ACTAGT 1 cut(s) 2944
AjnI CCWGG 7 cut(s) 586, 2350, 2626, 2956, 3268, 3549, 4042
AjuI GAANNNNNNNTTGG 4 cut(s) 1026, 1058, 3333, 3365
Alw21I GWGCWC 3 cut(s) 74, 2332, 3598
Alw26I GTCTC 5 cut(s) 959, 1181, 1788, 2352, 2718
AlwI GGATC 7 cut(s) 265, 934, 974, 1356, 1473, 1672, 3664
AlwNI CAGNNNCTG 3 cut(s) 2030, 2560, 4002
Ama87I CYCGRG 1 cut(s) 1922
Aor51HI AGCGCT 1 cut(s) 3222
AoxI GGCC 6 cut(s) 129, 580, 885, 1006, 1247, 3777
ApeKI GCWGC 9 cut(s) 11, 121, 186, 776, 2024, 3071, 3074, 3572, 3956
ArsI GACNNNNNNTTYG 2 cut(s) 3090, 3122
AsiGI ACCGGT 1 cut(s) 3326
Asp700I GAANNNNTTC 3 cut(s) 423, 1566, 2014
AspA2I CCTAGG 1 cut(s) 2314
AspLEI GCGC 7 cut(s) 162, 619, 1581, 1630, 2985, 3223, 3793
AsuC2I CCSGG 2 cut(s) 653, 3395
AsuHPI GGTGA 6 cut(s) 817, 2314, 3131, 3316, 3808, 4159
AvaI CYCGRG 1 cut(s) 1922
AvaII GGWCC 9 cut(s) 36, 87, 649, 1046, 2005, 3329, 3988, 4046, 4101
AvrII CCTAGG 1 cut(s) 2314
BalI TGGCCA 1 cut(s) 1008
BauI CACGAG 1 cut(s) 4037
BbsI GAAGAC 5 cut(s) 111, 710, 1115, 1380, 4106
Bbv12I GWGCWC 3 cut(s) 74, 2332, 3598
BbvI GCAGC 8 cut(s) 108, 198, 788, 2036, 3061, 3083, 3559, 3968
BceAI ACGGC 1 cut(s) 3005
BcgI CGANNNNNNTGC 2 cut(s) 2156, 2190
BciT130I CCWGG 7 cut(s) 588, 2352, 2628, 2958, 3270, 3551, 4044
BclI TGATCA 2 cut(s) 291, 3798
BcnI CCSGG 2 cut(s) 653, 3395
BcoDI GTCTC 5 cut(s) 959, 1181, 1788, 2352, 2718
BcuI ACTAGT 1 cut(s) 2944
BfoI RGCGCY 2 cut(s) 620, 3224
BfuAI ACCTGC 1 cut(s) 3784
BglI GCCNNNNNGGC 1 cut(s) 588
BglII AGATCT 1 cut(s) 2885
BlnI CCTAGG 1 cut(s) 2314
BmcAI AGTACT 1 cut(s) 1361
Bme1390I CCNGG 9 cut(s) 588, 653, 2352, 2628, 2958, 3270, 3395, 3551, 4044
Bme18I GGWCC 9 cut(s) 36, 87, 649, 1046, 2005, 3329, 3988, 4046, 4101
BmeT110I CYCGRG 1 cut(s) 1922
BmiI GGNNCC 2 cut(s) 1467, 3276
BmrFI CCNGG 9 cut(s) 588, 653, 2352, 2628, 2958, 3270, 3395, 3551, 4044
BmrI ACTGGG 2 cut(s) 3141, 3971
BmsI GCATC 6 cut(s) 65, 555, 1486, 2006, 3052, 3584
BmuI ACTGGG 2 cut(s) 3141, 3971
BoxI GACNNNNGTC 1 cut(s) 2241
BpiI GAAGAC 5 cut(s) 111, 710, 1115, 1380, 4106
BpmI CTGGAG 4 cut(s) 570, 2334, 2649, 3550
BpuEI CTTGAG 2 cut(s) 492, 629
BpuMI CCSGG 2 cut(s) 653, 3395
Bsa29I ATCGAT 1 cut(s) 1477
BsaAI YACGTR 1 cut(s) 1659
BsaI GGTCTC 1 cut(s) 1181
BsaJI CCNNGG 9 cut(s) 652, 943, 1243, 2001, 2180, 2314, 2351, 3269, 3550
BsaWI WCCGGW 1 cut(s) 3326
Bse118I RCCGGY 2 cut(s) 318, 3326
Bse1I ACTGG 9 cut(s) 771, 1367, 2617, 2710, 3147, 3533, 3878, 3977, 3996
Bse3DI GCAATG 2 cut(s) 1623, 1741
BseBI CCWGG 7 cut(s) 588, 2352, 2628, 2958, 3270, 3551, 4044
BseCI ATCGAT 1 cut(s) 1477
BseDI CCNNGG 9 cut(s) 652, 943, 1243, 2001, 2180, 2314, 2351, 3269, 3550
BseGI GGATG 9 cut(s) 570, 1668, 1839, 1997, 2689, 3023, 3046, 3318, 3575
BseMI GCAATG 2 cut(s) 1623, 1741
BseNI ACTGG 9 cut(s) 771, 1367, 2617, 2710, 3147, 3533, 3878, 3977, 3996
BseRI GAGGAG 2 cut(s) 3691, 3760
BseXI GCAGC 8 cut(s) 108, 198, 788, 2036, 3061, 3083, 3559, 3968
BseYI CCCAGC 1 cut(s) 3223
BshFI GGCC 6 cut(s) 131, 582, 887, 1008, 1249, 3779
BshTI ACCGGT 1 cut(s) 3326
BshVI ATCGAT 1 cut(s) 1477
BsiHKAI GWGCWC 3 cut(s) 74, 2332, 3598
BsiHKCI CYCGRG 1 cut(s) 1922
BsiSI CCGG 4 cut(s) 319, 652, 3327, 3394
BsiWI CGTACG 1 cut(s) 1655
BslFI GGGAC 2 cut(s) 839, 2374
BsmAI GTCTC 5 cut(s) 959, 1181, 1788, 2352, 2718
BsmFI GGGAC 2 cut(s) 839, 2374
BsmI GAATGC 2 cut(s) 3167, 4214
BsnI GGCC 6 cut(s) 131, 582, 887, 1008, 1249, 3779
Bso31I GGTCTC 1 cut(s) 1181
BsoBI CYCGRG 1 cut(s) 1922
Bsp1286I GDGCHC 3 cut(s) 74, 2332, 3598
Bsp1407I TGTACA 1 cut(s) 3917
Bsp19I CCATGG 1 cut(s) 1243
BspACI CCGC 4 cut(s) 132, 2070, 3303, 3730
BspANI GGCC 6 cut(s) 131, 582, 887, 1008, 1249, 3779
BspDI ATCGAT 1 cut(s) 1477
BspHI TCATGA 2 cut(s) 918, 2377
BspLI GGNNCC 2 cut(s) 1467, 3276
BspMAI CTGCAG 5 cut(s) 16, 79, 126, 1747, 3340
BspMI ACCTGC 1 cut(s) 3784
BspPI GGATC 7 cut(s) 265, 934, 974, 1356, 1473, 1672, 3664
BspTNI GGTCTC 1 cut(s) 1181
BsrBI CCGCTC 1 cut(s) 3730
BsrDI GCAATG 2 cut(s) 1623, 1741
BsrFI RCCGGY 2 cut(s) 318, 3326
BsrGI TGTACA 1 cut(s) 3917
BsrI ACTGG 9 cut(s) 771, 1367, 2617, 2710, 3147, 3533, 3878, 3977, 3996
BssAI RCCGGY 2 cut(s) 318, 3326
BssECI CCNNGG 9 cut(s) 652, 943, 1243, 2001, 2180, 2314, 2351, 3269, 3550
BssNAI GTATAC 2 cut(s) 1240, 3742
BssSI CACGAG 1 cut(s) 4037
BssT1I CCWWGG 5 cut(s) 943, 1243, 2001, 2180, 2314
Bst1107I GTATAC 2 cut(s) 1240, 3742
Bst2BI CACGAG 1 cut(s) 4037
Bst2UI CCWGG 7 cut(s) 588, 2352, 2628, 2958, 3270, 3551, 4044
Bst6I CTCTTC 2 cut(s) 1544, 2820
BstAPI GCANNNNNTGC 1 cut(s) 1553
BstAUI TGTACA 1 cut(s) 3917
BstBAI YACGTR 1 cut(s) 1659
BstDSI CCRYGG 1 cut(s) 1243
BstEII GGTNACC 1 cut(s) 3322
BstENI CCTNNNNNAGG 1 cut(s) 1328
BstF5I GGATG 9 cut(s) 570, 1668, 1839, 1997, 2689, 3023, 3046, 3318, 3575
BstH2I RGCGCY 2 cut(s) 620, 3224
BstHHI GCGC 7 cut(s) 162, 619, 1581, 1630, 2985, 3223, 3793
BstMAI GTCTC 5 cut(s) 959, 1181, 1788, 2352, 2718
BstMWI GCNNNNNNNGC 6 cut(s) 588, 1553, 2989, 3071, 3440, 3613
BstNI CCWGG 7 cut(s) 588, 2352, 2628, 2958, 3270, 3551, 4044
BstNSI RCATGY 5 cut(s) 56, 275, 958, 1516, 1551
BstPAI GACNNNNGTC 1 cut(s) 2241
BstPI GGTNACC 1 cut(s) 3322
BstSCI CCNGG 9 cut(s) 586, 651, 2350, 2626, 2956, 3268, 3393, 3549, 4042
BstV1I GCAGC 8 cut(s) 108, 198, 788, 2036, 3061, 3083, 3559, 3968
BstV2I GAAGAC 5 cut(s) 111, 710, 1115, 1380, 4106
BstX2I RGATCY 4 cut(s) 939, 979, 1677, 2885
BstXI CCANNNNNNTGG 1 cut(s) 1159
BstYI RGATCY 4 cut(s) 939, 979, 1677, 2885
BstZ17I GTATAC 2 cut(s) 1240, 3742
Bsu15I ATCGAT 1 cut(s) 1477
BsuRI GGCC 6 cut(s) 131, 582, 887, 1008, 1249, 3779
BsuTUI ATCGAT 1 cut(s) 1477
BtgI CCRYGG 1 cut(s) 1243
BtsCI GGATG 9 cut(s) 570, 1668, 1839, 1997, 2689, 3023, 3046, 3318, 3575
BtsI GCAGTG 6 cut(s) 72, 131, 1740, 1782, 3009, 3920
BveI ACCTGC 1 cut(s) 3784
CaiI CAGNNNCTG 3 cut(s) 2030, 2560, 4002
CciI TCATGA 2 cut(s) 918, 2377
CfoI GCGC 7 cut(s) 162, 619, 1581, 1630, 2985, 3223, 3793
Cfr10I RCCGGY 2 cut(s) 318, 3326
ClaI ATCGAT 1 cut(s) 1477
CseI GACGC 1 cut(s) 1877
CsiI ACCWGGT 1 cut(s) 2956
Csp6I GTAC 8 cut(s) 1360, 1366, 1516, 1656, 2954, 3766, 3918, 4157
CspAI ACCGGT 1 cut(s) 3326
CviQI GTAC 8 cut(s) 1360, 1366, 1516, 1656, 2954, 3766, 3918, 4157
DraI TTTAAA 3 cut(s) 2343, 3496, 4186
EaeI YGGCCR 1 cut(s) 1006
Eam1104I CTCTTC 2 cut(s) 1544, 2820
EarI CTCTTC 2 cut(s) 1544, 2820
Eco130I CCWWGG 5 cut(s) 943, 1243, 2001, 2180, 2314
Eco31I GGTCTC 1 cut(s) 1181
Eco32I GATATC 3 cut(s) 538, 1077, 2274
Eco47I GGWCC 9 cut(s) 36, 87, 649, 1046, 2005, 3329, 3988, 4046, 4101
Eco47III AGCGCT 1 cut(s) 3222
Eco57I CTGAAG 7 cut(s) 1109, 1157, 1445, 2417, 3708, 3809, 3916
Eco88I CYCGRG 1 cut(s) 1922
Eco91I GGTNACC 1 cut(s) 3322
EcoNI CCTNNNNNAGG 1 cut(s) 1328
EcoO109I RGGNCCY 1 cut(s) 36
EcoO65I GGTNACC 1 cut(s) 3322
EcoRI GAATTC 1 cut(s) 2168
EcoRII CCWGG 7 cut(s) 586, 2350, 2626, 2956, 3268, 3549, 4042
EcoRV GATATC 3 cut(s) 538, 1077, 2274
EcoT14I CCWWGG 5 cut(s) 943, 1243, 2001, 2180, 2314
EcoT22I ATGCAT 2 cut(s) 58, 4216
ErhI CCWWGG 5 cut(s) 943, 1243, 2001, 2180, 2314
FalI AAGNNNNNCTT 4 cut(s) 1536, 1568, 3333, 3365
FaqI GGGAC 2 cut(s) 839, 2374
FauI CCCGC 1 cut(s) 3310
FauNDI CATATG 1 cut(s) 2764
FbaI TGATCA 2 cut(s) 291, 3798
FblI GTMKAC 4 cut(s) 1239, 1401, 2544, 3741
FokI GGATG 9 cut(s) 577, 1675, 1826, 1984, 2696, 3010, 3053, 3325, 3562
FspI TGCGCA 1 cut(s) 3792
GlaI GCGC 7 cut(s) 161, 618, 1580, 1629, 2984, 3222, 3792
GsaI CCCAGC 1 cut(s) 3227
GsuI CTGGAG 4 cut(s) 570, 2334, 2649, 3550
HaeII RGCGCY 2 cut(s) 620, 3224
HaeIII GGCC 6 cut(s) 131, 582, 887, 1008, 1249, 3779
HapII CCGG 4 cut(s) 319, 652, 3327, 3394
HgaI GACGC 1 cut(s) 1877
HhaI GCGC 7 cut(s) 162, 619, 1581, 1630, 2985, 3223, 3793
Hin6I GCGC 7 cut(s) 160, 617, 1579, 1628, 2983, 3221, 3791
HinP1I GCGC 7 cut(s) 160, 617, 1579, 1628, 2983, 3221, 3791
HincII GTYRAC 2 cut(s) 1402, 2545
HindII GTYRAC 2 cut(s) 1402, 2545
HindIII AAGCTT 2 cut(s) 466, 3242
HinfI GANTC 8 cut(s) 484, 568, 860, 1148, 1849, 2474, 2828, 3380
HpaII CCGG 4 cut(s) 319, 652, 3327, 3394
HphI GGTGA 6 cut(s) 817, 2314, 3131, 3316, 3808, 4159
Hpy166II GTNNAC 5 cut(s) 1240, 1402, 2545, 3742, 3920
Hpy8I GTNNAC 5 cut(s) 1240, 1402, 2545, 3742, 3920
HpyCH4IV ACGT 3 cut(s) 152, 1658, 2854
HpyF10VI GCNNNNNNNGC 6 cut(s) 588, 1553, 2989, 3071, 3440, 3613
HpySE526I ACGT 3 cut(s) 152, 1658, 2854
HspAI GCGC 7 cut(s) 160, 617, 1579, 1628, 2983, 3221, 3791
Ksp22I TGATCA 2 cut(s) 291, 3798
Lsp1109I GCAGC 8 cut(s) 108, 198, 788, 2036, 3061, 3083, 3559, 3968
LweI GCATC 6 cut(s) 65, 555, 1486, 2006, 3052, 3584
MabI ACCWGGT 1 cut(s) 2956
MaeII ACGT 3 cut(s) 152, 1658, 2854
MbiI CCGCTC 1 cut(s) 3730
MfeI CAATTG 2 cut(s) 969, 1130
MflI RGATCY 4 cut(s) 939, 979, 1677, 2885
MhlI GDGCHC 3 cut(s) 74, 2332, 3598
MlsI TGGCCA 1 cut(s) 1008
MluNI TGGCCA 1 cut(s) 1008
MlyI GAGTC 3 cut(s) 478, 562, 2837
MmeI TCCRAC 2 cut(s) 1417, 3881
Mox20I TGGCCA 1 cut(s) 1008
Mph1103I ATGCAT 2 cut(s) 58, 4216
MroXI GAANNNNTTC 3 cut(s) 423, 1566, 2014
MscI TGGCCA 1 cut(s) 1008
MslI CAYNNNNRTG 3 cut(s) 1389, 1494, 3388
Msp20I TGGCCA 1 cut(s) 1008
MspA1I CMGCKG 2 cut(s) 3074, 3426
MspI CCGG 4 cut(s) 319, 652, 3327, 3394
MspR9I CCNGG 9 cut(s) 588, 653, 2352, 2628, 2958, 3270, 3395, 3551, 4044
MunI CAATTG 2 cut(s) 969, 1130
Mva1269I GAATGC 2 cut(s) 3167, 4214
MvaI CCWGG 7 cut(s) 588, 2352, 2628, 2958, 3270, 3551, 4044
MwoI GCNNNNNNNGC 6 cut(s) 588, 1553, 2989, 3071, 3440, 3613
NciI CCSGG 2 cut(s) 653, 3395
NcoI CCATGG 1 cut(s) 1243
NdeI CATATG 1 cut(s) 2764
NlaIV GGNNCC 2 cut(s) 1467, 3276
NmuCI GTSAC 3 cut(s) 823, 2795, 3322
NsbI TGCGCA 1 cut(s) 3792
NsiI ATGCAT 2 cut(s) 58, 4216
NspI RCATGY 5 cut(s) 56, 275, 958, 1516, 1551
PaeI GCATGC 2 cut(s) 56, 1551
PagI TCATGA 2 cut(s) 918, 2377
PaqCI CACCTGC 1 cut(s) 3784
PciI ACATGT 2 cut(s) 954, 1512
PctI GAATGC 2 cut(s) 3167, 4214
PdmI GAANNNNTTC 3 cut(s) 423, 1566, 2014
PfeI GAWTC 5 cut(s) 860, 1148, 1849, 2474, 3380
Pfl23II CGTACG 1 cut(s) 1655
PflMI CCANNNNNTGG 2 cut(s) 593, 1333
PinAI ACCGGT 1 cut(s) 3326
PleI GAGTC 3 cut(s) 478, 562, 2836
PpsI GAGTC 3 cut(s) 478, 562, 2836
Ppu21I YACGTR 1 cut(s) 1659
PpuMI RGGWCCY 1 cut(s) 36
PscI ACATGT 2 cut(s) 954, 1512
PshAI GACNNNNGTC 1 cut(s) 2241
PsiI TTATAA 1 cut(s) 678
Psp1406I AACGTT 1 cut(s) 152
Psp5II RGGWCCY 1 cut(s) 36
Psp6I CCWGG 7 cut(s) 586, 2350, 2626, 2956, 3268, 3549, 4042
PspEI GGTNACC 1 cut(s) 3322
PspFI CCCAGC 1 cut(s) 3223
PspGI CCWGG 7 cut(s) 586, 2350, 2626, 2956, 3268, 3549, 4042
PspLI CGTACG 1 cut(s) 1655
PspN4I GGNNCC 2 cut(s) 1467, 3276
PspPPI RGGWCCY 1 cut(s) 36
PstI CTGCAG 5 cut(s) 16, 79, 126, 1747, 3340
PstNI CAGNNNCTG 3 cut(s) 2030, 2560, 4002
PsuI RGATCY 4 cut(s) 939, 979, 1677, 2885
PvuII CAGCTG 2 cut(s) 3074, 3426
RsaI GTAC 8 cut(s) 1361, 1367, 1517, 1657, 2955, 3767, 3919, 4158
RsaNI GTAC 8 cut(s) 1360, 1366, 1516, 1656, 2954, 3766, 3918, 4157
RseI CAYNNNNRTG 3 cut(s) 1389, 1494, 3388
SalI GTCGAC 2 cut(s) 1400, 2543
ScaI AGTACT 1 cut(s) 1361
SchI GAGTC 3 cut(s) 478, 562, 2837
ScrFI CCNGG 9 cut(s) 588, 653, 2352, 2628, 2958, 3270, 3395, 3551, 4044
SduI GDGCHC 3 cut(s) 74, 2332, 3598
SexAI ACCWGGT 1 cut(s) 2956
SfaNI GCATC 6 cut(s) 65, 555, 1486, 2006, 3052, 3584
SinI GGWCC 9 cut(s) 36, 87, 649, 1046, 2005, 3329, 3988, 4046, 4101
SmiMI CAYNNNNRTG 3 cut(s) 1389, 1494, 3388
SmlI CTYRAG 2 cut(s) 507, 644
SmoI CTYRAG 2 cut(s) 507, 644
SpeI ACTAGT 1 cut(s) 2944
SphI GCATGC 2 cut(s) 56, 1551
SsiI CCGC 4 cut(s) 132, 2070, 3303, 3730
SspI AATATT 1 cut(s) 2537
StyD4I CCNGG 9 cut(s) 586, 651, 2350, 2626, 2956, 3268, 3393, 3549, 4042
StyI CCWWGG 5 cut(s) 943, 1243, 2001, 2180, 2314
TaiI ACGT 3 cut(s) 155, 1661, 2857
TaqII GACCGA 1 cut(s) 4063
TatI WGTACW 5 cut(s) 1359, 1515, 3765, 3917, 4156
TauI GCSGC 2 cut(s) 134, 2073
TfiI GAWTC 5 cut(s) 860, 1148, 1849, 2474, 3380
TseFI GTSAC 3 cut(s) 823, 2795, 3322
TseI GCWGC 9 cut(s) 11, 121, 186, 776, 2024, 3071, 3074, 3572, 3956
Tsp45I GTSAC 3 cut(s) 823, 2795, 3322
TspGWI ACGGA 2 cut(s) 1280, 1976
Van91I CCANNNNNTGG 2 cut(s) 593, 1333
VpaK11BI GGWCC 9 cut(s) 36, 87, 649, 1046, 2005, 3329, 3988, 4046, 4101
XagI CCTNNNNNAGG 1 cut(s) 1328
XceI RCATGY 5 cut(s) 56, 275, 958, 1516, 1551
XcmI CCANNNNNNNNNTGG 1 cut(s) 3034
XmaJI CCTAGG 1 cut(s) 2314
XmiI GTMKAC 4 cut(s) 1239, 1401, 2544, 3741
XmnI GAANNNNTTC 3 cut(s) 423, 1566, 2014
ZrmI AGTACT 1 cut(s) 1361
Zsp2I ATGCAT 2 cut(s) 58, 4216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.