Rh2AG611000

pleiotropic drug resistance protein 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000472
Physical Location & Seq
Forward (+)
83739291 .. 83754958
15668 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG611000.1

Sequence Viewer

Length: 4314 bp
ATGGATTTGGCTGCAGAGATAGGAAGAAGTGTGAGGACTTCTTTTCATCAGCATGCATCAAGTTTCAGGAGCACTTCAGATGTCAGGTCCATGAAAGAGAATGATGACGATGAAGTTGAGTTGCAGTGGGCTGCCATTGAAAGATTGCCCACATTTGAACGTATTCGAACTTCACTCTTCGGTTGTAACAAGGAGGACGATGATCATGAAGGTAAGAGTAAGAGGGTAGTTGATGTTACAAAGCTAGAAGCCCTGGAACGTCGTGTGTTCATTGATAAACTTCTCAAGCAAATAGAAGGAGATAATCAATGCCTCTTACAGAAACTCAAAGAAAGAATAGACAGAGTGGGACTGCAATTGCCGACTGTGGAAGTACGATACCAGAATTTGTTTGTAGAAGCAGAATGTGAAGTAGTTCAAGGAAAGCCCCTCCCAACACTATGGAACACGCTCAAAAGCTTTCTCTCTGTCATGACACAGATAATTGGATGCAAGTCTGAAGCATACAATCTAGAAATTCTCAAAGATGTCAGTGGCATCATCAAGCCTTCAAGGATGACTCTTTTGCTTGGTCCTCCAGGCTGTGGAAAAACTACCTTATTACAGGCACTTGCAGGAAAACTTAACCACCCTCTCAAGGTTCAAGGAGAAATAACCTACAATGGTTATAAGCTCAATGAGTTTGTTCCTCAGAAGACATCAGCTTACATAAGTCAATATGACTTGCATATATCTGAGATGACTGTGAGGGAAACACTGGATTTCTCATCTCGTTGCCAGGGCATCGGAAGCAGAGCTGATATTATGAAAGAAGTTGCTAGAAGGGAGAAGCAAGCTGGTATTGTCCCAGAACCAGATATTGACACTTATATGAAGGCCATTTCAATTGAAGGGTTGAAAAAATCTCTAATGACAGACTATATAATAAAGATCCTTGGGCTGGACATTTGTGCTGAGACAATCACAGGGGACCCAATGCAAAGAGGAATTTCGGGTGGTCAAAAGAAAAGGCTAACAACAGGTGAGATGATGATTGGCTCAGCAAGAGCTTTCTTCATGGATGAGATATCAACAGGACTAGACAGCTCCACTACATTTCAGATTGTAACTTGTCTGCAGCAATTGACTCACGTAACAGAATCGTCCATTTTGGTTTCGCTTCTTCAGCCAACGCCAGAGACCTTTCATCTGTTTGATGACATTATCTTGATGGCTGAAGGAAAAGTTGTATACCATGGGCCTAGTGACAATGTTGTGGAGTTCTTTCAGAAGTGTGGTTTCCTTAGCCCACCACGAAAAGGCATTTCTGACTTCCTCCAAGAGGTGGTTTCTCAAAAGGATCAAGCAAAGTACTGGTACCATAAAGACCAACCCTACAGTTATGTTAGTGTTGATGAGTTCGTAAGTATGTTTAAGGACTTCCATGTCGGAAAGAAGCTTGAAGAGGAACTTTCCAAGCCTTTTGAGCAATCAGAGTGCCATAAGCATGCTTTGTCATACAACATTTACTCACTGAGAAAATGGGAACTATTCAAAGCATGCCTTTCTAGAGAATGGCTTCTAATGAAGCGAAACTCTTTTGTTCATGTATTCCAATCAGTACAGCTTGTGGTCATTGCACTAGTAACAATGACAATGTTTCTACGTACATGGATGAAAGTCAACGAGATCTATGCAAAGTACTACATGAGTGCTTTGTTTTATGCTCTAATTAGATTTCTATGCAATGCAAGTTCAGAGATATCAATGACTTCCTCTAGACTTGCAGTCTTCTATAAGCAAAGAGATGTCTACTTTTACCCTGCATGGGCTTATTCTATTCCGGCTGCCATCCTGAAGATTCCATTTTCATTGCTAGATGCATTTCTTTGGACAGCCCTTACTTATTATGTGATTGGTTACAGTCCTGAACCTGAAAGGTTCTTCAGGCAATTTATTCTTCTGTTCCTCGTGCATCAAGTAGCGATATCACTATTTCGTTTGATTGCATCCTTGGTCCGAAATCCTTCTGTTGCAGGATCTGTCGGCCTTTTATCTTTAATAGTAATGTTTCTATTTGGAGGCTTCATAATACCAAAATCTTCTCTACCTGCTTGGTTGGAATGGGGATTTTGGGTTTCTCCATTGGCCTTTGCAGAACTAGGTGTTTCAATAAATGAATTTCATGCTCCAAGGTGGCAAAAGGTTTTATCTTCCAATGTCACTATAGGCCAGCAAGCTCTAATTAATCGTGGTCTAAACTTCAATGATTACTTCTATTGGATATCCATAGGAGCTTTAATTGGATTTTGGATGGTTTTCAACCTTGGGTTCACACGGGCATTGAGTTATTCAAAGTCTCCAGGTAGTTCTCGGACTATCATTTCTCATGAAAAATTCTCCCGTCTGAAGAAAAAAGAAATTTTAGATAATTGCGGTCAAGAAAGAGAATTAACTAGTGTAGATACTGATATAACTACTTCGGAATCTACAAATGCAGGCATGGTGTTGCCTTTTGAGCCCATTACTATATCATTTGAGAAACTGCAATACTTTATTGATACACCCCAGAAACTGAGAGAGCAAGGTTTCCCACCAAATCGACTACAGCTTCTTCAAGATATAACTGGTGCATTCAGACCTGGCGTTCTTACAGCACTGATGGGTGTTAGTGGAGCAGGAAAGACAACACTGATGGATGTTCTTTCTGGAAGGAAAACTGGTGGACTTATTGAAGGAGACATTAGAATTAGAGGACACCCAAAAGTCCAAGAAACATATGCCAGAATATCTGGGTATTGCGAGCAAAGTGATATACATTCTCCAGAGCTTACAGTAGAAGAGTCAGTAGCATACTCAGCTTGGTTACGCTTGCCATCCCAGATTGCTAGAAATACTAGAGCTGAGTTTGTGAAAGAAGTACTTCATATGATAGAGCTTGATGAAATCAAAGATGAGTTAGTTGGCATACCAGGTGGTAGTGGTATATCGACTGAACAGCGTAAAAGGCTAACAATAGCAGTAGAGCTTGTTTCAAATCCATCCGTTATATTTATGGATGAACCCACATCTGGTTTAGATGCCAGAGCAGCTGCAATAGTTATGAGAGTTGTGAAAAATATTGTCAGTACAAGGAGGACTGTTGTTTGCACTATTCATCAGCCAAGTATTGACATATTTGAAGCATTCGATGAGCTTCTTTTGATGAAAAGAGGAGGACAAGTAATATACTGTGGAGAGCTGGGTCAAGATTCAAGTAAGCTTATCCAATACTTTCAGGGTATTTCTGGGGTTCCAAAAATCAAAGATAATTACAACCCAGCAACATGGATGTTAGAAGTCACAAGTCCTTCTGCAGAAGCCAAACTTGCCTTAGATTTTGCTCAGCTTTATAGAGAATCAAATCTATGCCAGAAGAACAATGACCTAGTTAGAGAACACAGCTTACCAGCACAAGGCTCAACGGGATTGGATTTTCCTACTCGTTTCCCACAAAATGGATGGGAACAATTTAAAGCATGTCTGTGGAAGCGACATCTCTCCTACTGGAGAAATCCAAAATATAACCTGGGGCGATTGTTAATCACTGCTGCATCCTCTTTGATATTTGGAGCACTTCTTTGGCAGAAAGGACAGAAAATAAATGGTGAACAGGAGTTCTTCAGCATTCTAGGATCAATATTTATTTTGCAACAAAACATGGGAATTGGTAATTGTTCCTCTGTTTTACCATTTGTCGCTTCTGAGCGCAATGTTGTATATCGAGAAAGGTTTGCTGGAATGTACTCCTCATGGGCATATTCTTTATCACAGGTGATCATTGAACTTCCTTATGTATTTTTTCAAGCAGTTTTGTTTTCGACCATTACATATCCATCGATTGGTTTCCACTGGTCACTTTACAAAGTATTCTGGTATGTGTATGCTATGTTTTTCACGCTGCTCTACTTCACTTACTTCGGTATATTGGTTGCTTCTGTGACCCCAACTTTCCAAGTAGCTTCAGTATTAGCAAGTTTCTGCTACACCATGCTCAATCTGTTCTCGGGATACCTAATACCTCAACCAAACATTCCTAAATGGTGGGTTTGGGGCTACTATATTTGCCCTTTATCGTGGTCCTTAAAAGGTATTCTCACTTCACAGTACGGAGATCTAAACACGGAAATAGTACTCCGCGGGGAAGAAAAGACGATCAGTGCCTTCTTAGACAGCATCTATGGATACAACTATGATGATCTAGGAATTGTAGGAATTTTTCTTCTTGCATATCCACTAGTTTTCGCATTTGCGTTTGCACTTGCTACTGAAAAACTAAACTTCCAGAGGACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1437

Amino Acids

163.08

Weight (kDa)

7.27

Isoelectric Point (pI)

39.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 174 - 355 2e-15 ABC transporter
ABC2_membrane PF01061 510 - 721 2.4e-41 ABC-2 type transporter
PDR_assoc PF08370 726 - 788 6.5e-20 Plant PDR ABC transporter associated
ABC_tran PF00005 865 - 1017 5.5e-20 ABC transporter
ABC2_membrane PF01061 1162 - 1376 2.6e-56 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000472)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15230 AT4G15230 AT4G15230 AT4G15230 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15236
fragaria_vesca FvH4_4g21360 FvH4_4g21360 FvH4_4g21390 FvH4_6g49431 FvH4_6g49450
malus_domestica MD09G1044300.v1.1 MD16G1137900.v1.1 MD16G1138000.v1.1
prunus_persica Prupe.1G106800_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.3G273300_v2.0.a1
pyrus_communis pycom111g03460 pycom16g12210
rosa_chinensis RchiOBHm_Chr2g0169331 RchiOBHm_Chr2g0169361 RchiOBHm_Chr2g0169371 RchiOBHm_Chr2g0169381 RchiOBHm_Chr2g0169411 RchiOBHm_Chr4g0427141 RchiOBHm_Chr4g0427321
rosa_laevigata RLG00000007252 RLG00000007258 RLG00000013974 RLG00000021856 RLG00000021857 RLG00000021858 RLG00000021859
rosa_multiflora Rmu_sc0000648.1_g000007 Rmu_sc0000648.1_g000009 Rmu_sc0003813.1_g000016 Rmu_sc0009165.1_g000008 Rmu_sc0022554.1_g000001
rosa_roxburghii Rroxscaffold_2G00082200 Rroxscaffold_2G00082230 Rroxscaffold_5G00368960 Rroxscaffold_5G00369010 Rroxscaffold_7G00200390
rosa_rugosa Rorug02G0539500 Rorug02G0539600 Rorug02G0539700 Rorug04G0216400 Rorug04G0216500 Rorug04G0216500 Rorug04G0216600
rosa_samantha Rh2AG610800 Rh2AG611000 Rh2AG611100 Rh2CG592800 Rh2CG593000 Rh2CG593200 Rh2DG634900 Rh2DG635100 Rh2DG635200 Rh2DG635400 Rh4AG272200 Rh4AG272900 Rh4DG275600 Rh4DG276000
rosa_wichuraiana Rw2G050640 Rw2G050660 Rw2G050670 Rw2G050680 Rw4G023610 Rw4G023680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 669
AasI GACNNNNNNGTC 1 cut(s) 1424
Acc36I ACCTGC 1 cut(s) 2098
Acc65I GGTACC 1 cut(s) 1356
AccB1I GGYRCC 1 cut(s) 1356
AccB7I CCANNNNNTGG 4 cut(s) 584, 1324, 3476, 3860
AccI GTMKAC 2 cut(s) 1230, 1791
AccII CGCG 1 cut(s) 4158
AciI CCGC 3 cut(s) 2415, 4156, 4158
AclWI GGATC 4 cut(s) 925, 1347, 2026, 3661
AcsI RAATTY 7 cut(s) 385, 516, 987, 2159, 2375, 2400, 4233
AcuI CTGAAG 9 cut(s) 60, 519, 1148, 1236, 1856, 1909, 2408, 3625, 3966
AhdI GACNNNNNGTC 1 cut(s) 1766
AhlI ACTAGT 3 cut(s) 1621, 2435, 4255
AjnI CCWGG 7 cut(s) 252, 577, 777, 2341, 2620, 2950, 3546
Alw21I GWGCWC 2 cut(s) 74, 3595
Alw26I GTCTC 4 cut(s) 950, 1172, 2343, 2712
AlwI GGATC 4 cut(s) 925, 1347, 2026, 3661
AlwNI CAGNNNCTG 2 cut(s) 2021, 2554
Ama87I CYCGRG 1 cut(s) 4024
AoxI GGCC 5 cut(s) 876, 1238, 2026, 2127, 2209
ApeKI GCWGC 8 cut(s) 11, 131, 1117, 1826, 3068, 3071, 3569, 3919
ApoI RAATTY 7 cut(s) 385, 516, 987, 2159, 2375, 2400, 4233
AseI ATTAAT 1 cut(s) 2226
Asp700I GAANNNNTTC 5 cut(s) 162, 414, 1557, 2005, 2901
Asp718I GGTACC 1 cut(s) 1356
AspLEI GCGC 1 cut(s) 3729
AspS9I GGNCC 6 cut(s) 87, 572, 970, 1238, 1996, 4098
AsuHPI GGTGA 3 cut(s) 1034, 3638, 3805
AsuII TTCGAA 1 cut(s) 166
AvaI CYCGRG 1 cut(s) 4024
AvaII GGWCC 5 cut(s) 87, 572, 970, 1996, 4098
BanI GGYRCC 1 cut(s) 1356
BanII GRGCYC 1 cut(s) 2502
BarI GAAGNNNNNNTAC 4 cut(s) 154, 186, 4102, 4134
BauI CACGAG 1 cut(s) 1949
BbsI GAAGAC 2 cut(s) 701, 1762
Bbv12I GWGCWC 2 cut(s) 74, 3595
BbvI GCAGC 7 cut(s) 118, 1129, 1813, 3058, 3080, 3556, 3906
BccI CCATC 9 cut(s) 1204, 1838, 2287, 2635, 2668, 2863, 3028, 3474, 3862
BcgI CGANNNNNNTGC 2 cut(s) 1655, 1689
BciT130I CCWGG 7 cut(s) 254, 579, 779, 2343, 2622, 2952, 3548
BciVI GTATCC 2 cut(s) 4022, 4196
BclI TGATCA 2 cut(s) 202, 3795
BcoDI GTCTC 4 cut(s) 950, 1172, 2343, 2712
BcuI ACTAGT 3 cut(s) 1621, 2435, 4255
BfmI CTRYAG 6 cut(s) 12, 1115, 1375, 2205, 2585, 3333
BfuAI ACCTGC 1 cut(s) 2098
BfuI GTATCC 2 cut(s) 4022, 4196
BglII AGATCT 2 cut(s) 1668, 4132
BisI GCNGC 8 cut(s) 12, 132, 1118, 1827, 3069, 3072, 3570, 3920
BlpI GCTNAGC 2 cut(s) 1039, 3363
BlsI GCNGC 8 cut(s) 13, 133, 1119, 1828, 3070, 3073, 3571, 3921
BmcAI AGTACT 4 cut(s) 1352, 1682, 2901, 4152
Bme1390I CCNGG 7 cut(s) 254, 579, 779, 2343, 2622, 2952, 3548
Bme18I GGWCC 5 cut(s) 87, 572, 970, 1996, 4098
BmeRI GACNNNNNGTC 1 cut(s) 1766
BmeT110I CYCGRG 1 cut(s) 4024
BmgT120I GGNCC 6 cut(s) 87, 572, 970, 1238, 1996, 4098
BmiI GGNNCC 4 cut(s) 971, 972, 1358, 3273
BmrFI CCNGG 7 cut(s) 254, 579, 779, 2343, 2622, 2952, 3548
BpiI GAAGAC 2 cut(s) 701, 1762
BpmI CTGGAG 4 cut(s) 561, 2325, 2787, 3547
Bpu10I CCTNAGC 1 cut(s) 1283
Bpu1102I GCTNAGC 2 cut(s) 1039, 3363
Bpu14I TTCGAA 1 cut(s) 166
BpuEI CTTGAG 2 cut(s) 269, 620
Bsa29I ATCGAT 1 cut(s) 3857
BsaAI YACGTR 2 cut(s) 1132, 1646
BsaI GGTCTC 1 cut(s) 1172
BsaJI CCNNGG 9 cut(s) 252, 778, 934, 1234, 1992, 2171, 2305, 3547, 4156
BsaXI ACNNNNNCTCC 2 cut(s) 3186, 3216
Bse1I ACTGG 6 cut(s) 762, 1358, 2611, 2704, 3530, 3875
Bse3DI GCAATG 4 cut(s) 1614, 1732, 1850, 3736
BseBI CCWGG 7 cut(s) 254, 579, 779, 2343, 2622, 2952, 3548
BseCI ATCGAT 1 cut(s) 3857
BseDI CCNNGG 9 cut(s) 252, 778, 934, 1234, 1992, 2171, 2305, 3547, 4156
BseMI GCAATG 4 cut(s) 1614, 1732, 1850, 3736
BseNI ACTGG 6 cut(s) 762, 1358, 2611, 2704, 3530, 3875
BseRI GAGGAG 2 cut(s) 3207, 3757
BseXI GCAGC 7 cut(s) 118, 1129, 1813, 3058, 3080, 3556, 3906
BseYI CCCAGC 2 cut(s) 3220, 3298
Bsh1236I CGCG 1 cut(s) 4158
BshFI GGCC 5 cut(s) 878, 1240, 2028, 2129, 2211
BshNI GGYRCC 1 cut(s) 1356
BshVI ATCGAT 1 cut(s) 3857
BsiHKAI GWGCWC 2 cut(s) 74, 3595
BsiHKCI CYCGRG 1 cut(s) 4024
BsiSI CCGG 1 cut(s) 1823
BslFI GGGAC 3 cut(s) 363, 830, 983
BsmAI GTCTC 4 cut(s) 950, 1172, 2343, 2712
BsmFI GGGAC 3 cut(s) 363, 830, 983
BsmI GAATGC 3 cut(s) 2612, 3164, 3645
BsnI GGCC 5 cut(s) 878, 1240, 2028, 2129, 2211
Bso31I GGTCTC 1 cut(s) 1172
BsoBI CYCGRG 1 cut(s) 4024
Bsp119I TTCGAA 1 cut(s) 166
Bsp1286I GDGCHC 3 cut(s) 74, 2502, 3595
Bsp1720I GCTNAGC 2 cut(s) 1039, 3363
Bsp19I CCATGG 1 cut(s) 1234
BspACI CCGC 3 cut(s) 2415, 4156, 4158
BspANI GGCC 5 cut(s) 878, 1240, 2028, 2129, 2211
BspDI ATCGAT 1 cut(s) 3857
BspFNI CGCG 1 cut(s) 4158
BspHI TCATGA 3 cut(s) 205, 471, 2368
BspLI GGNNCC 4 cut(s) 971, 972, 1358, 3273
BspMAI CTGCAG 3 cut(s) 16, 1119, 3337
BspMI ACCTGC 1 cut(s) 2098
BspPI GGATC 4 cut(s) 925, 1347, 2026, 3661
BspT104I TTCGAA 1 cut(s) 166
BspT107I GGYRCC 1 cut(s) 1356
BspTNI GGTCTC 1 cut(s) 1172
BsrDI GCAATG 4 cut(s) 1614, 1732, 1850, 3736
BsrI ACTGG 6 cut(s) 762, 1358, 2611, 2704, 3530, 3875
BssECI CCNNGG 9 cut(s) 252, 778, 934, 1234, 1992, 2171, 2305, 3547, 4156
BssNAI GTATAC 1 cut(s) 1231
BssSI CACGAG 1 cut(s) 1949
BssT1I CCWWGG 5 cut(s) 934, 1234, 1992, 2171, 2305
Bst1107I GTATAC 1 cut(s) 1231
Bst2BI CACGAG 1 cut(s) 1949
Bst2UI CCWGG 7 cut(s) 254, 579, 779, 2343, 2622, 2952, 3548
Bst4CI ACNGT 8 cut(s) 367, 745, 1379, 1904, 2815, 3121, 3212, 4125
Bst6I CTCTTC 3 cut(s) 182, 1437, 2814
BstBAI YACGTR 2 cut(s) 1132, 1646
BstBI TTCGAA 1 cut(s) 166
BstC8I GCNNGC 9 cut(s) 54, 834, 1488, 1540, 2213, 2217, 2479, 2783, 2852
BstDSI CCRYGG 2 cut(s) 1234, 4156
BstENI CCTNNNNNAGG 1 cut(s) 1319
BstFNI CGCG 1 cut(s) 4158
BstHHI GCGC 1 cut(s) 3729
BstMAI GTCTC 4 cut(s) 950, 1172, 2343, 2712
BstMWI GCNNNNNNNGC 8 cut(s) 789, 1165, 1465, 2497, 2837, 2986, 3068, 3347
BstNI CCWGG 7 cut(s) 254, 579, 779, 2343, 2622, 2952, 3548
BstNSI RCATGY 4 cut(s) 56, 1490, 1542, 3501
BstSCI CCNGG 7 cut(s) 252, 577, 777, 2341, 2620, 2950, 3546
BstSFI CTRYAG 6 cut(s) 12, 1115, 1375, 2205, 2585, 3333
BstSNI TACGTA 1 cut(s) 1646
BstUI CGCG 1 cut(s) 4158
BstV1I GCAGC 7 cut(s) 118, 1129, 1813, 3058, 3080, 3556, 3906
BstV2I GAAGAC 2 cut(s) 701, 1762
BstX2I RGATCY 4 cut(s) 930, 1668, 2018, 4132
BstXI CCANNNNNNTGG 2 cut(s) 441, 3306
BstYI RGATCY 4 cut(s) 930, 1668, 2018, 4132
BstZ17I GTATAC 1 cut(s) 1231
Bsu15I ATCGAT 1 cut(s) 3857
BsuI GTATCC 2 cut(s) 4022, 4196
BsuRI GGCC 5 cut(s) 878, 1240, 2028, 2129, 2211
BsuTUI ATCGAT 1 cut(s) 3857
BtgI CCRYGG 2 cut(s) 1234, 4156
BtsI GCAGTG 2 cut(s) 131, 3564
BtsIMutI CAGTG 9 cut(s) 131, 538, 755, 1511, 2636, 2669, 3564, 3868, 4183
BveI ACCTGC 1 cut(s) 2098
Cac8I GCNNGC 9 cut(s) 54, 834, 1488, 1540, 2213, 2217, 2479, 2783, 2852
CaiI CAGNNNCTG 2 cut(s) 2021, 2554
CciI TCATGA 3 cut(s) 205, 471, 2368
CfoI GCGC 1 cut(s) 3729
Cfr13I GGNCC 6 cut(s) 87, 572, 970, 1238, 1996, 4098
Cfr42I CCGCGG 1 cut(s) 4159
ClaI ATCGAT 1 cut(s) 3857
CsiI ACCWGGT 1 cut(s) 2950
DraI TTTAAA 1 cut(s) 3493
DrdI GACNNNNNNGTC 1 cut(s) 1424
DriI GACNNNNNGTC 1 cut(s) 1766
DseDI GACNNNNNNGTC 1 cut(s) 1424
Eam1104I CTCTTC 3 cut(s) 182, 1437, 2814
Eam1105I GACNNNNNGTC 1 cut(s) 1766
EarI CTCTTC 3 cut(s) 182, 1437, 2814
Eco105I TACGTA 1 cut(s) 1646
Eco130I CCWWGG 5 cut(s) 934, 1234, 1992, 2171, 2305
Eco24I GRGCYC 1 cut(s) 2502
Eco31I GGTCTC 1 cut(s) 1172
Eco32I GATATC 4 cut(s) 1068, 1743, 1968, 2265
Eco47I GGWCC 5 cut(s) 87, 572, 970, 1996, 4098
Eco57I CTGAAG 9 cut(s) 60, 519, 1148, 1236, 1856, 1909, 2408, 3625, 3966
Eco88I CYCGRG 1 cut(s) 4024
EcoNI CCTNNNNNAGG 1 cut(s) 1319
EcoO109I RGGNCCY 1 cut(s) 970
EcoRII CCWGG 7 cut(s) 252, 577, 777, 2341, 2620, 2950, 3546
EcoRV GATATC 4 cut(s) 1068, 1743, 1968, 2265
EcoT14I CCWWGG 5 cut(s) 934, 1234, 1992, 2171, 2305
EcoT22I ATGCAT 2 cut(s) 58, 1864
EcoT38I GRGCYC 1 cut(s) 2502
ErhI CCWWGG 5 cut(s) 934, 1234, 1992, 2171, 2305
FalI AAGNNNNNCTT 8 cut(s) 1434, 1466, 1527, 1559, 2886, 2918, 3330, 3362
FaqI GGGAC 3 cut(s) 363, 830, 983
FauI CCCGC 1 cut(s) 4151
FauNDI CATATG 2 cut(s) 2758, 2907
FbaI TGATCA 2 cut(s) 202, 3795
FblI GTMKAC 2 cut(s) 1230, 1791
Fnu4HI GCNGC 8 cut(s) 12, 132, 1118, 1827, 3069, 3072, 3570, 3920
FriOI GRGCYC 1 cut(s) 2502
Fsp4HI GCNGC 8 cut(s) 12, 132, 1118, 1827, 3069, 3072, 3570, 3920
GlaI GCGC 1 cut(s) 3728
GluI GCNGC 8 cut(s) 12, 132, 1118, 1827, 3069, 3072, 3570, 3920
GsaI CCCAGC 2 cut(s) 3224, 3302
GsuI CTGGAG 4 cut(s) 561, 2325, 2787, 3547
HaeIII GGCC 5 cut(s) 878, 1240, 2028, 2129, 2211
HapII CCGG 1 cut(s) 1823
HhaI GCGC 1 cut(s) 3729
Hin6I GCGC 1 cut(s) 3727
HinP1I GCGC 1 cut(s) 3727
HincII GTYRAC 1 cut(s) 1663
HindII GTYRAC 1 cut(s) 1663
HindIII AAGCTT 3 cut(s) 457, 1436, 3239
HinfI GANTC 8 cut(s) 559, 1126, 1139, 1840, 2465, 2822, 3230, 3377
HpaII CCGG 1 cut(s) 1823
HphI GGTGA 3 cut(s) 1034, 3638, 3805
Hpy166II GTNNAC 6 cut(s) 1231, 1663, 1792, 2313, 2705, 3629
Hpy8I GTNNAC 6 cut(s) 1231, 1663, 1792, 2313, 2705, 3629
Hpy99I CGWCG 1 cut(s) 264
HpyCH4III ACNGT 8 cut(s) 367, 745, 1379, 1904, 2815, 3121, 3212, 4125
HpyCH4IV ACGT 5 cut(s) 160, 259, 1131, 1645, 4310
HpyF10VI GCNNNNNNNGC 8 cut(s) 789, 1165, 1465, 2497, 2837, 2986, 3068, 3347
HpySE526I ACGT 5 cut(s) 160, 259, 1131, 1645, 4310
HspAI GCGC 1 cut(s) 3727
KflI GGGWCCC 1 cut(s) 970
KpnI GGTACC 1 cut(s) 1360
Ksp22I TGATCA 2 cut(s) 202, 3795
KspI CCGCGG 1 cut(s) 4159
LmnI GCTCC 6 cut(s) 69, 1091, 2173, 2273, 2654, 3590
Lsp1109I GCAGC 7 cut(s) 118, 1129, 1813, 3058, 3080, 3556, 3906
MabI ACCWGGT 1 cut(s) 2950
MaeII ACGT 5 cut(s) 160, 259, 1131, 1645, 4310
MfeI CAATTG 3 cut(s) 356, 885, 1121
MflI RGATCY 4 cut(s) 930, 1668, 2018, 4132
MhlI GDGCHC 3 cut(s) 74, 2502, 3595
MlyI GAGTC 3 cut(s) 553, 1120, 2831
MmeI TCCRAC 2 cut(s) 1408, 2079
Mph1103I ATGCAT 2 cut(s) 58, 1864
MroXI GAANNNNTTC 5 cut(s) 162, 414, 1557, 2005, 2901
MseI TTAA 9 cut(s) 624, 1413, 2039, 2226, 2279, 2432, 3492, 3560, 4103
MslI CAYNNNNRTG 4 cut(s) 51, 869, 1485, 3502
MspA1I CMGCKG 2 cut(s) 3071, 4158
MspI CCGG 1 cut(s) 1823
MspR9I CCNGG 7 cut(s) 254, 579, 779, 2343, 2622, 2952, 3548
MunI CAATTG 3 cut(s) 356, 885, 1121
Mva1269I GAATGC 3 cut(s) 2612, 3164, 3645
MvaI CCWGG 7 cut(s) 254, 579, 779, 2343, 2622, 2952, 3548
MvnI CGCG 1 cut(s) 4158
MwoI GCNNNNNNNGC 8 cut(s) 789, 1165, 1465, 2497, 2837, 2986, 3068, 3347
NcoI CCATGG 1 cut(s) 1234
NdeI CATATG 2 cut(s) 2758, 2907
NlaIV GGNNCC 4 cut(s) 971, 972, 1358, 3273
NmuCI GTSAC 5 cut(s) 1244, 2200, 3319, 3873, 3958
NsiI ATGCAT 2 cut(s) 58, 1864
NspI RCATGY 4 cut(s) 56, 1490, 1542, 3501
NspV TTCGAA 1 cut(s) 166
PaeI GCATGC 3 cut(s) 56, 1490, 1542
PagI TCATGA 3 cut(s) 205, 471, 2368
PctI GAATGC 3 cut(s) 2612, 3164, 3645
PdmI GAANNNNTTC 5 cut(s) 162, 414, 1557, 2005, 2901
PfeI GAWTC 5 cut(s) 1139, 1840, 2465, 3230, 3377
PflMI CCANNNNNTGG 4 cut(s) 584, 1324, 3476, 3860
PkrI GCNGC 8 cut(s) 13, 133, 1119, 1828, 3070, 3073, 3571, 3921
PleI GAGTC 3 cut(s) 553, 1120, 2830
PpsI GAGTC 3 cut(s) 553, 1120, 2830
Ppu21I YACGTR 2 cut(s) 1132, 1646
PpuMI RGGWCCY 1 cut(s) 970
PshBI ATTAAT 1 cut(s) 2226
PsiI TTATAA 1 cut(s) 669
Psp5II RGGWCCY 1 cut(s) 970
Psp6I CCWGG 7 cut(s) 252, 577, 777, 2341, 2620, 2950, 3546
PspFI CCCAGC 2 cut(s) 3220, 3298
PspGI CCWGG 7 cut(s) 252, 577, 777, 2341, 2620, 2950, 3546
PspN4I GGNNCC 4 cut(s) 971, 972, 1358, 3273
PspPI GGNCC 6 cut(s) 87, 572, 970, 1238, 1996, 4098
PspPPI RGGWCCY 1 cut(s) 970
PsrI GAACNNNNNNTAC 2 cut(s) 3408, 3440
PstI CTGCAG 3 cut(s) 16, 1119, 3337
PstNI CAGNNNCTG 2 cut(s) 2021, 2554
PsuI RGATCY 4 cut(s) 930, 1668, 2018, 4132
PvuII CAGCTG 1 cut(s) 3071
RseI CAYNNNNRTG 4 cut(s) 51, 869, 1485, 3502
SacII CCGCGG 1 cut(s) 4159
SaqAI TTAA 9 cut(s) 624, 1413, 2039, 2226, 2279, 2432, 3492, 3560, 4103
SatI GCNGC 8 cut(s) 12, 132, 1118, 1827, 3069, 3072, 3570, 3920
Sau96I GGNCC 6 cut(s) 87, 572, 970, 1238, 1996, 4098
ScaI AGTACT 4 cut(s) 1352, 1682, 2901, 4152
SchI GAGTC 3 cut(s) 553, 1120, 2831
ScrFI CCNGG 7 cut(s) 254, 579, 779, 2343, 2622, 2952, 3548
SduI GDGCHC 3 cut(s) 74, 2502, 3595
SexAI ACCWGGT 1 cut(s) 2950
SfcI CTRYAG 6 cut(s) 12, 1115, 1375, 2205, 2585, 3333
Sfr303I CCGCGG 1 cut(s) 4159
SfuI TTCGAA 1 cut(s) 166
SgrBI CCGCGG 1 cut(s) 4159
SinI GGWCC 5 cut(s) 87, 572, 970, 1996, 4098
SmiMI CAYNNNNRTG 4 cut(s) 51, 869, 1485, 3502
SmlI CTYRAG 2 cut(s) 284, 635
SmoI CTYRAG 2 cut(s) 284, 635
SnaBI TACGTA 1 cut(s) 1646
SpeI ACTAGT 3 cut(s) 1621, 2435, 4255
SphI GCATGC 3 cut(s) 56, 1490, 1542
SsiI CCGC 3 cut(s) 2415, 4156, 4158
SspI AATATT 2 cut(s) 3100, 3660
StyD4I CCNGG 7 cut(s) 252, 577, 777, 2341, 2620, 2950, 3546
StyI CCWWGG 5 cut(s) 934, 1234, 1992, 2171, 2305
TaaI ACNGT 8 cut(s) 367, 745, 1379, 1904, 2815, 3121, 3212, 4125
TaiI ACGT 5 cut(s) 163, 262, 1134, 1648, 4313
TaqI TCGA 7 cut(s) 166, 2581, 2969, 3168, 3742, 3839, 3857
TatI WGTACW 7 cut(s) 1350, 1600, 1680, 2899, 3107, 3762, 4150
TfiI GAWTC 5 cut(s) 1139, 1840, 2465, 3230, 3377
Tru1I TTAA 9 cut(s) 624, 1413, 2039, 2226, 2279, 2432, 3492, 3560, 4103
Tru9I TTAA 9 cut(s) 624, 1413, 2039, 2226, 2279, 2432, 3492, 3560, 4103
TscAI CASTG 9 cut(s) 131, 538, 762, 1518, 2643, 2676, 3571, 3875, 4183
TseFI GTSAC 5 cut(s) 1244, 2200, 3319, 3873, 3958
TseI GCWGC 8 cut(s) 11, 131, 1117, 1826, 3068, 3071, 3569, 3919
Tsp45I GTSAC 5 cut(s) 1244, 2200, 3319, 3873, 3958
TspGWI ACGGA 3 cut(s) 3013, 4143, 4157
TspRI CASTG 9 cut(s) 131, 538, 762, 1518, 2643, 2676, 3571, 3875, 4183
Van91I CCANNNNNTGG 4 cut(s) 584, 1324, 3476, 3860
VpaK11BI GGWCC 5 cut(s) 87, 572, 970, 1996, 4098
VspI ATTAAT 1 cut(s) 2226
XagI CCTNNNNNAGG 1 cut(s) 1319
XapI RAATTY 7 cut(s) 385, 516, 987, 2159, 2375, 2400, 4233
XbaI TCTAGA 3 cut(s) 511, 1547, 1757
XceI RCATGY 4 cut(s) 56, 1490, 1542, 3501
XcmI CCANNNNNNNNNTGG 1 cut(s) 3477
XmiI GTMKAC 2 cut(s) 1230, 1791
XmnI GAANNNNTTC 5 cut(s) 162, 414, 1557, 2005, 2901
ZrmI AGTACT 4 cut(s) 1352, 1682, 2901, 4152
Zsp2I ATGCAT 2 cut(s) 58, 1864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.