Rh2CG593200

pleiotropic drug resistance protein 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000472
Physical Location & Seq
Forward (+)
76940859 .. 76965482
24624 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG593200.1

Sequence Viewer

Length: 4806 bp
ATGGCCATAGTCATATTCCATAAAACTAGATTTAACTTGGAAGCTGTTGCAACAACAGATATAATGAAAGAAGTCACCAGAAGGGAGAAGCAAGAAGGAATTGTCCCAGATTCAGATATTGATACTTATATGAAGGTGGTTTCCCAAAAGGATCAAGCAAAGTACTGGTACGATGAAGACCAACCCCATAGTTATGTTACTGTCGACAACTTTGTAGATATGTTCAAGGACTTCCATGTTGGAAAGAAGCTCGATGAGGAACTTCAGGAGCCTTTCAATCGATCCGAGTTCCATAAAGATGCTTTGTCATTTAACATTTACTCATTGACAAAATGGGAACTCTTCAAAGCATGCCTTGCTAGAGAATGGCTTCTGATGAAGCGCAACTCGTTTGTCCATGTATTCAAATCAGCACAGCTCTCATCTTCAAATATCACAATAGGTCAGCAAGCTCTAAAAGACAGTGATCTTGATTTCAGTGAATACTTCTATTGGATATCTATAGGAGCTTTACTTGGATTTTGGTTGGTTTTCAACCTAGGTTTCACCTGTGCTCTCAGTTATTTAAAGTCTCCAGGGAGTTCTCGAACAATCATTTCTCATGAAAGGTTGTCCCGTCTGAAGGAAAAGGAAAGTCTAAACAATTCCCCTCGAAAAAAAAAATTAACTCGTATAGAAACTCGAATAAGAGTTGAAGATTCTACAAATATAGGGGTCTTACCTTTTGAACCCTTGACTATATCATTTGAGAAGGTGCAATATTTTGTCGACTTACCCCAGAAACTGAGAGAGCAAGGTGTTCCACCAAAACGACTACAACTTCTTCAAGATATAACTGGTGCATTTAGACCTGGAGTTCTTACAGCACTTATGGGTGTTAGTGGAGCTGGAAAGACAACACTGATGGATGTTCTTTCAGGAAGGAAAACTGGTGGAATTATTGAAGGAGACATTAGAATTGGAGGACACCCAAAAGTCCAAGAAACATATGCCAGAATATCTGGGTATTGCGAGCAAAGTGACATACATTCCCCACAGATAACAGTAGAAGAGTCGGTAACATACTCAGCTTGGTTACGTTTGCCAGCACAGGTTGATAGAACAGTCAGATCTGAATTTGTGAGAGAAGTTCTACAAATGATCGAGCTTGATGAAATCAAGGACGAACTAGTGGGCGTACCTGGTAGGGGTATATCGACTGAACAGCTCAAACGGCTTACTATAGCAGTGGAGCTTGTTTCAAATCCATCCATAATGTTCATGGATGAACCCACCTCTGGTTTAGATGCCAGAGCAGCTGCAATAGTTATGCGAGTGGTCAAAAACATTGTCAGCACTAGGAGGACTGTTGTATGCACCATTCACCAGCCCAGTATTGACATCTTTGAAGCATTCGATGAGCTTATTTTAATGAAAATAGGAGGACAGCTAATCTACAGTGGAGCGCTGGGTCAAAATTCAAGCAAGCTTATTGAATACTTTGAGCGTATTCCTGGGGTTCCAAACATCAAAGAAAATTACAACCCCGCAACATGGATGTTAGAGGTCACCGGTCCTTCTGCAGAAGCTAAACTTGGCTTAGATTTTGCTCAACTCTATAGAGAATCACATCTATGCCGGGAGAACGATGACCTAGTTAGAGAACTCAGCTATCCAGCAGAAGGGGCAAAGAAACTACATTTTTCTACTCGCTTTGCACAAAATGAATGGGAGCAATTTAAAGCCTGCCTCTGGAAGCAACATCTCTCCTACTGGAGAAATCCACAATATAACCTGGGCAGATTGACAATTACTGCTGCATCCTCTTTGTTATTCGGAGCACTTCTTTGGCAGAAAGGGCAGACAGTAGATAATGAACAGGATTTTTTCAACATTCTGGGATCAATGTTTGTTTTACTGCAATTCATGGGAATAGGAAACTGCTCCTCTGTTTTACCCTGTATTGCTTCAGAGCGGAATGTTGTATACCGAGAAAGGTTTGCTGGAATGTACTCCTCATGGGCCTATTCATTTGCGCAGGTGATCATTGAAATTCCATACATATTTCTTCAGGCAGTTTTGTTTTTGGCAATCACATATCCAACCATAAATTTCCACTGGACACTTTACCAAGTGTTTTGGTATGTCTATGCCATGTTTTGTACACTGCTCTACTTCAGTTACTTCGGCATATTGCTTGCAGCTTTGACCCCAACATTCCCAGTGGCTATGGTCCTAGCCAGTTTCTGTTACACCATGTTCAACTTATTCTCAGGTTTCCTCGTGCCTGGACCGAAAATTCCTAAATGGTGGGTTTGGGGTTATTGGATTTGCCCTGTAGCATGGTCCTTAAACGGTCTTCTCACTTCACAGTATGGAGATCAAAACAAGGTGATAGTAGTACATGGGGAGAAAAAGACCATCAGTGACTTCTTAGAAAGTATTCATGGCCTTTTGATCATTTATCACGGGTCACCCTTCAAGCCAAAGACCCCACTTTCATTCTGTCAATACAATGGAAGTGTTTGCTGTAATTCCACTGATGATATGCAACTGCAAAATCTATTTACGGCTATGAATGTTTCTGATCTCAGTTGTGCTTCTCTTCTGAAATCTATACTTTGCTCGAGATGTGATCAGTTTTCCGCAGATCTATACAGAATGGAATCAGTGCCTCGTGAAGTTCCTGTTCTCTGCAACTCAACTGTTTCATCAAACTCAACCCAATACCAACACAGTGGAGCTGACTTTTGTACCAAGGTTTGGGATGAATGCCAAAATGTATCTGTATTGAGTTCTCCATTTGCGTTGCAAGGTAGAGGTGGAACACCATTTAATTCTACTTCCAAGCTGAGTGATATATGGCAGTCAAAAAGTGCTTTCTGTGATACATTTGGTGGATCTTCTGATGGGGAATTGGTCTGTTTTGATGGTGGACCTGTGTTTCTGAACAACACTGAAATTCCAAGTCCCCCAAGTGGCATGTGCCTTGAAAAAATTGGAAATGGATCTTATCTTAACATGGTACCTCATCCAGATGGGTCTAATCGCGTGTTCTTATCTGATCAACCGGGGAAAGTATGGCTGGCAACTGTCCCTGAGGAGGGATCAGGAGAAACGTTGGTGATTGATGATTCAAATCCGTTTCTTGATCTAACTGATCAAGTGTATGCTGACACTGAGTTTGGAATGATGGGATTAGCATTCCATCCTAATTATCTACAGAATGGCCGTTTCTTTGCTTCATTTAACTGTGATAAGGTTAGATGGCCAGAATGTTCAGGAAGATGTTCGTGTAACTCAGATGTGGGATGTGATCCTTCCAAGCTTGGTTCTGATAATGGTGCTCAGCCATGTCAATATCACAGTATCATAGCAGAGTTCACTGCTAATGGTTCCGCATCACAACCTTCTTCAGTCATCACTAGTGTTAAACCATTGGAAGTCAGAAGAATTTTCACTATGGGACTTCCATTTACTTCCCATCATGGAGGCCAAATTCTTTTTGGACCCAAAGACGGGTATTTGTACTTCATGATGGGAGATGGTGGTAGCGTAGGTGACCCTTACAATTTTTCACAAAACAAGAAATCCTTGCTTGGAAAGATTATGAGGCTAGACATAGATTACTTACCAAGTGCATTCTCAATTAGTGATCTTGGCCTATGGGGAAACTATTCTGTTCCTGGAGATAATCCGTTTTCTGAAGATAAGGAGTTGCAGCCTGAAATTTGGGCTCTAGGATTTAGAAATCCTTGGCGATGTAGTTTTGATCTGGAAAGACCTTCCTACTTCCTTTGTGCTGATGTTGGTCAGGACAAATATGAGGAGGTTGATATAGTCACCAAGGGTGGAAACTACGGATGGCGTGCATATGAGGGTGCTTTTCCATACCATCCCCCAAAGTCTCCAGGAGGAAATACATCTATGAGTTCTATCAACCCGATTTTCCCTGTAATGGGATACAACCACTCTGATGTCAACAAGGCTGAAGGCTCTGCATCAATCACTGGTGGCTACTTTTACCGCTCCATGACAGATCCATGCATGTATGGAAGGTATCTTTATGCGGATTTGTATGCTGGTCACATATGGGCAGGTACAGAAACTCCTGAAGATAGCGGAAACTTCACAAGTACTATAGTTCCTGTCAGTTGTGCTCTGGACTCTCCTATCGAGTGCAGAGCAGAGGCAGGAAGCTCTTTTCCTGCATTGGGGTTCATATTCTCCTTTGGTCAGGATAACAGGAAAGACATGTTTATCTTAGCCAGTACCGGTGTATATAGAGTTGCTAGGCCTAGTCGGTGCAACTACACTTGCTCCAAAGAAAATGCTTCATCTTTTTCCCATCCAGGTTCTGTTCCTTCTCCTTCTCCCTCCACTTCAAGCACTAGGAAGCTGAACAACCCAGTTATCGAGACACAGTTTCAGCAGAACCTTCAGATGATTAAAGACCTGGAGGAGCCTATCAGAAGGTCATATGTTCAGGCTCGTGATAAAGAAAGGTCCCTTATTGCTGAAATAGAAGCAATACGGAGAAAGAACAATGCTCAGGCGCAAATTATAGCAGAGCTGCAGCAAAATTCCAATTCCAATTTCCCCTTGTATGCATTAGACTCGGCAACAGATGTTCTACTGGATGGAAGAGGGCCTGAAAATTTTGATGGATATGATGATTACAAAACCCTGCTCAGTTTTGTTCCTGAGAATGAGATGATAATTGGGGATGCTACTGTCAATCATGAGTTGAAGGCAACAATGCAATTTCTTAATGTTAGATCCAGGGATTTCAATGATCCAGTGAGCAGTGAATCGTCCAGGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1601

Amino Acids

179.64

Weight (kDa)

5.45

Isoelectric Point (pI)

42.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PDR_assoc PF08370 141 - 199 1.1e-20 Plant PDR ABC transporter associated
ABC_tran PF00005 275 - 426 2.9e-19 ABC transporter
ABC2_membrane PF01061 571 - 785 2.3e-58 ABC-2 type transporter
ABC2_membrane_3 PF12698 620 - 787 1.3e-06 ABC-2 family transporter protein
GSDH PF07995 1010 - 1309 5.4e-20 Glucose / Sorbosone dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000472)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15230 AT4G15230 AT4G15230 AT4G15230 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15236
fragaria_vesca FvH4_4g21360 FvH4_4g21360 FvH4_4g21390 FvH4_6g49431 FvH4_6g49450
malus_domestica MD09G1044300.v1.1 MD16G1137900.v1.1 MD16G1138000.v1.1
prunus_persica Prupe.1G106800_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.3G273300_v2.0.a1
pyrus_communis pycom111g03460 pycom16g12210
rosa_chinensis RchiOBHm_Chr2g0169331 RchiOBHm_Chr2g0169361 RchiOBHm_Chr2g0169371 RchiOBHm_Chr2g0169381 RchiOBHm_Chr2g0169411 RchiOBHm_Chr4g0427141 RchiOBHm_Chr4g0427321
rosa_laevigata RLG00000007252 RLG00000007258 RLG00000013974 RLG00000021856 RLG00000021857 RLG00000021858 RLG00000021859
rosa_multiflora Rmu_sc0000648.1_g000007 Rmu_sc0000648.1_g000009 Rmu_sc0003813.1_g000016 Rmu_sc0009165.1_g000008 Rmu_sc0022554.1_g000001
rosa_roxburghii Rroxscaffold_2G00082200 Rroxscaffold_2G00082230 Rroxscaffold_5G00368960 Rroxscaffold_5G00369010 Rroxscaffold_7G00200390
rosa_rugosa Rorug02G0539500 Rorug02G0539600 Rorug02G0539700 Rorug04G0216400 Rorug04G0216500 Rorug04G0216500 Rorug04G0216600
rosa_samantha Rh2AG610800 Rh2AG611000 Rh2AG611100 Rh2CG592800 Rh2CG593000 Rh2CG593200 Rh2DG634900 Rh2DG635100 Rh2DG635200 Rh2DG635400 Rh4AG272200 Rh4AG272900 Rh4DG275600 Rh4DG276000
rosa_wichuraiana Rw2G050640 Rw2G050660 Rw2G050670 Rw2G050680 Rw4G023610 Rw4G023680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2008
Acc16I TGCGCA 1 cut(s) 2016
Acc36I ACCTGC 2 cut(s) 2008, 4067
Acc65I GGTACC 1 cut(s) 3004
AccB1I GGYRCC 1 cut(s) 3004
AccB7I CCANNNNNTGG 1 cut(s) 2742
AccBSI CCGCTC 2 cut(s) 1954, 4008
AccI GTMKAC 3 cut(s) 204, 768, 1965
AccII CGCG 1 cut(s) 3030
AciI CCGC 7 cut(s) 1527, 1954, 2625, 3378, 4006, 4049, 4101
AclI AACGTT 1 cut(s) 3098
AcoI YGGCCR 3 cut(s) 3, 3208, 3248
AfeI AGCGCT 1 cut(s) 1446
AflIII ACRYGT 1 cut(s) 4233
AgeI ACCGGT 2 cut(s) 1550, 4253
AhlI ACTAGT 2 cut(s) 1168, 3404
AjuI GAANNNNNNNTTGG 4 cut(s) 1557, 1589, 4559, 4591
Alw21I GWGCWC 4 cut(s) 556, 1822, 3328, 4141
Alw26I GTCTC 4 cut(s) 576, 942, 3891, 4391
AlwNI CAGNNNCTG 3 cut(s) 784, 2226, 4337
Ama87I CYCGRG 1 cut(s) 2605
Aor51HI AGCGCT 1 cut(s) 1446
AoxI GGCC 9 cut(s) 3, 2001, 2428, 3208, 3248, 3472, 3640, 4274, 4628
ApeKI GCWGC 7 cut(s) 1295, 1298, 1796, 2180, 3700, 4552, 4555
ArsI GACNNNNNNTTYG 4 cut(s) 1314, 1346, 3146, 3178
AsiGI ACCGGT 2 cut(s) 1550, 4253
Asp700I GAANNNNTTC 4 cut(s) 369, 2421, 3268, 3655
Asp718I GGTACC 1 cut(s) 3004
AspA2I CCTAGG 1 cut(s) 538
AspLEI GCGC 4 cut(s) 384, 1447, 2017, 4537
AspS9I GGNCC 9 cut(s) 1553, 2001, 2212, 2270, 2325, 2915, 3488, 4485, 4628
AsuC2I CCSGG 2 cut(s) 1619, 3051
AvaI CYCGRG 1 cut(s) 2605
AvaII GGWCC 7 cut(s) 1553, 2212, 2270, 2325, 2915, 3488, 4485
AvrII CCTAGG 1 cut(s) 538
AxyI CCTNAGG 1 cut(s) 3078
BaeI ACNNNNGTAYC 2 cut(s) 2716, 2749
BalI TGGCCA 2 cut(s) 5, 3250
BanI GGYRCC 1 cut(s) 3004
BanII GRGCYC 1 cut(s) 3718
BarI GAAGNNNNNNTAC 2 cut(s) 3494, 3526
BauI CACGAG 3 cut(s) 2261, 2655, 4470
BbsI GAAGAC 2 cut(s) 183, 2330
Bbv12I GWGCWC 4 cut(s) 556, 1822, 3328, 4141
BbvI GCAGC 7 cut(s) 1285, 1307, 1783, 2192, 3712, 4539, 4567
BceAI ACGGC 3 cut(s) 1229, 2565, 3195
BcgI CGANNNNNNTGC 2 cut(s) 4578, 4612
BciVI GTATCC 1 cut(s) 3935
BclI TGATCA 5 cut(s) 2022, 2436, 2614, 3043, 3139
BcnI CCSGG 2 cut(s) 1619, 3051
BcoDI GTCTC 4 cut(s) 576, 942, 3891, 4391
BcuI ACTAGT 2 cut(s) 1168, 3404
BfmI CTRYAG 9 cut(s) 501, 1221, 1435, 1560, 1597, 2316, 3200, 4119, 4553
BfoI RGCGCY 1 cut(s) 1448
BfuAI ACCTGC 2 cut(s) 2008, 4067
BfuI GTATCC 1 cut(s) 3935
BglII AGATCT 2 cut(s) 1109, 2629
BisI GCNGC 7 cut(s) 1296, 1299, 1797, 2181, 3701, 4553, 4556
BlnI CCTAGG 1 cut(s) 538
BlpI GCTNAGC 1 cut(s) 3327
BlsI GCNGC 7 cut(s) 1297, 1300, 1798, 2182, 3702, 4554, 4557
BmcAI AGTACT 2 cut(s) 164, 4117
Bme18I GGWCC 7 cut(s) 1553, 2212, 2270, 2325, 2915, 3488, 4485
BmeT110I CYCGRG 1 cut(s) 2605
BmgT120I GGNCC 9 cut(s) 1553, 2001, 2212, 2270, 2325, 2915, 3488, 4485, 4628
BmiI GGNNCC 7 cut(s) 270, 1500, 3006, 3376, 3490, 4443, 4487
BmrI ACTGGG 3 cut(s) 1365, 2195, 4382
BmsI GCATC 6 cut(s) 289, 1276, 1808, 3389, 3989, 4696
BmuI ACTGGG 3 cut(s) 1365, 2195, 4382
BpiI GAAGAC 2 cut(s) 183, 2330
BpmI CTGGAG 6 cut(s) 558, 873, 1774, 3687, 3873, 4457
Bpu10I CCTNAGC 1 cut(s) 4530
Bpu1102I GCTNAGC 1 cut(s) 3327
BpuMI CCSGG 2 cut(s) 1619, 3051
Bsa29I ATCGAT 1 cut(s) 280
BsaBI GATNNNNATC 1 cut(s) 3117
BsaJI CCNNGG 9 cut(s) 538, 575, 1493, 1774, 2736, 3050, 3734, 3825, 4761
BsaWI WCCGGW 2 cut(s) 1550, 4253
BsaXI ACNNNNNCTCC 4 cut(s) 4072, 4102, 4283, 4313
Bse118I RCCGGY 2 cut(s) 1550, 4253
Bse21I CCTNAGG 1 cut(s) 3078
Bse8I GATNNNNATC 1 cut(s) 3117
BseCI ATCGAT 1 cut(s) 280
BseDI CCNNGG 9 cut(s) 538, 575, 1493, 1774, 2736, 3050, 3734, 3825, 4761
BseJI GATNNNNATC 1 cut(s) 3117
BseRI GAGGAG 5 cut(s) 1915, 1984, 3095, 3821, 4454
BseXI GCAGC 7 cut(s) 1285, 1307, 1783, 2192, 3712, 4539, 4567
BseYI CCCAGC 1 cut(s) 1447
BsgI GTGCAG 1 cut(s) 4180
Bsh1236I CGCG 1 cut(s) 3030
BshFI GGCC 9 cut(s) 5, 2003, 2430, 3210, 3250, 3474, 3642, 4276, 4630
BshNI GGYRCC 1 cut(s) 3004
BshTI ACCGGT 2 cut(s) 1550, 4253
BshVI ATCGAT 1 cut(s) 280
BsiHKAI GWGCWC 4 cut(s) 556, 1822, 3328, 4141
BsiHKCI CYCGRG 1 cut(s) 2605
BsiSI CCGG 4 cut(s) 1551, 1618, 3050, 4254
BslFI GGGAC 6 cut(s) 89, 598, 2934, 3059, 3459, 4471
BsmAI GTCTC 4 cut(s) 576, 942, 3891, 4391
BsmFI GGGAC 6 cut(s) 89, 598, 2934, 3059, 3459, 4471
BsmI GAATGC 4 cut(s) 1391, 2756, 3182, 3620
BsnI GGCC 9 cut(s) 5, 2003, 2430, 3210, 3250, 3474, 3642, 4276, 4630
BsoBI CYCGRG 1 cut(s) 2605
Bsp1286I GDGCHC 5 cut(s) 556, 1822, 3328, 3718, 4141
Bsp1407I TGTACA 1 cut(s) 2141
Bsp1720I GCTNAGC 1 cut(s) 3327
BspACI CCGC 7 cut(s) 1527, 1954, 2625, 3378, 4006, 4049, 4101
BspANI GGCC 9 cut(s) 5, 2003, 2430, 3210, 3250, 3474, 3642, 4276, 4630
BspDI ATCGAT 1 cut(s) 280
BspFNI CGCG 1 cut(s) 3030
BspHI TCATGA 3 cut(s) 601, 3513, 4720
BspLI GGNNCC 7 cut(s) 270, 1500, 3006, 3376, 3490, 4443, 4487
BspMAI CTGCAG 2 cut(s) 1564, 4557
BspMI ACCTGC 2 cut(s) 2008, 4067
BspT107I GGYRCC 1 cut(s) 3004
BsrBI CCGCTC 2 cut(s) 1954, 4008
BsrFI RCCGGY 2 cut(s) 1550, 4253
BsrGI TGTACA 1 cut(s) 2141
BssAI RCCGGY 2 cut(s) 1550, 4253
BssECI CCNNGG 9 cut(s) 538, 575, 1493, 1774, 2736, 3050, 3734, 3825, 4761
BssNAI GTATAC 1 cut(s) 1966
BssSI CACGAG 3 cut(s) 2261, 2655, 4470
BssT1I CCWWGG 4 cut(s) 538, 2736, 3734, 3825
Bst1107I GTATAC 1 cut(s) 1966
Bst2BI CACGAG 3 cut(s) 2261, 2655, 4470
Bst6I CTCTTC 4 cut(s) 347, 1044, 2589, 4618
BstAPI GCANNNNNTGC 1 cut(s) 356
BstAUI TGTACA 1 cut(s) 2141
BstC8I GCNNGC 9 cut(s) 352, 450, 1013, 1086, 1466, 1726, 2178, 3066, 3849
BstEII GGTNACC 3 cut(s) 1546, 2451, 3539
BstFNI CGCG 1 cut(s) 3030
BstH2I RGCGCY 1 cut(s) 1448
BstHHI GCGC 4 cut(s) 384, 1447, 2017, 4537
BstMAI GTCTC 4 cut(s) 576, 942, 3891, 4391
BstMWI GCNNNNNNNGC 5 cut(s) 356, 1213, 1295, 1664, 1837
BstNSI RCATGY 4 cut(s) 354, 2965, 4030, 4237
BstPI GGTNACC 3 cut(s) 1546, 2451, 3539
BstSFI CTRYAG 9 cut(s) 501, 1221, 1435, 1560, 1597, 2316, 3200, 4119, 4553
BstUI CGCG 1 cut(s) 3030
BstV1I GCAGC 7 cut(s) 1285, 1307, 1783, 2192, 3712, 4539, 4567
BstV2I GAAGAC 2 cut(s) 183, 2330
BstX2I RGATCY 6 cut(s) 1109, 2629, 2879, 2987, 4018, 4757
BstXI CCANNNNNNTGG 1 cut(s) 2717
BstYI RGATCY 6 cut(s) 1109, 2629, 2879, 2987, 4018, 4757
BstZ17I GTATAC 1 cut(s) 1966
Bsu15I ATCGAT 1 cut(s) 280
Bsu36I CCTNAGG 1 cut(s) 3078
BsuI GTATCC 1 cut(s) 3935
BsuRI GGCC 9 cut(s) 5, 2003, 2430, 3210, 3250, 3474, 3642, 4276, 4630
BsuTUI ATCGAT 1 cut(s) 280
BtgZI GCGATG 1 cut(s) 3754
BtsI GCAGTG 4 cut(s) 1233, 2144, 3363, 4792
BveI ACCTGC 2 cut(s) 2008, 4067
Cac8I GCNNGC 9 cut(s) 352, 450, 1013, 1086, 1466, 1726, 2178, 3066, 3849
CaiI CAGNNNCTG 3 cut(s) 784, 2226, 4337
CciI TCATGA 3 cut(s) 601, 3513, 4720
CfoI GCGC 4 cut(s) 384, 1447, 2017, 4537
Cfr10I RCCGGY 2 cut(s) 1550, 4253
Cfr13I GGNCC 9 cut(s) 1553, 2001, 2212, 2270, 2325, 2915, 3488, 4485, 4628
ClaI ATCGAT 1 cut(s) 280
CsiI ACCWGGT 1 cut(s) 1180
CspAI ACCGGT 2 cut(s) 1550, 4253
DraI TTTAAA 2 cut(s) 567, 1720
EaeI YGGCCR 3 cut(s) 3, 3208, 3248
Eam1104I CTCTTC 4 cut(s) 347, 1044, 2589, 4618
EarI CTCTTC 4 cut(s) 347, 1044, 2589, 4618
Eco130I CCWWGG 4 cut(s) 538, 2736, 3734, 3825
Eco147I AGGCCT 1 cut(s) 4276
Eco24I GRGCYC 1 cut(s) 3718
Eco32I GATATC 1 cut(s) 498
Eco47I GGWCC 7 cut(s) 1553, 2212, 2270, 2325, 2915, 3488, 4485
Eco47III AGCGCT 1 cut(s) 1446
Eco81I CCTNAGG 1 cut(s) 3078
Eco88I CYCGRG 1 cut(s) 2605
Eco91I GGTNACC 3 cut(s) 1546, 2451, 3539
EcoO109I RGGNCCY 2 cut(s) 4485, 4628
EcoO65I GGTNACC 3 cut(s) 1546, 2451, 3539
EcoRV GATATC 1 cut(s) 498
EcoT14I CCWWGG 4 cut(s) 538, 2736, 3734, 3825
EcoT22I ATGCAT 2 cut(s) 4028, 4591
EcoT38I GRGCYC 1 cut(s) 3718
ErhI CCWWGG 4 cut(s) 538, 2736, 3734, 3825
FalI AAGNNNNNCTT 6 cut(s) 339, 371, 1557, 1589, 3557, 3589
FaqI GGGAC 6 cut(s) 89, 598, 2934, 3059, 3459, 4471
FauI CCCGC 1 cut(s) 1534
FauNDI CATATG 4 cut(s) 988, 3853, 4070, 4459
FbaI TGATCA 5 cut(s) 2022, 2436, 2614, 3043, 3139
FblI GTMKAC 3 cut(s) 204, 768, 1965
Fnu4HI GCNGC 7 cut(s) 1296, 1299, 1797, 2181, 3701, 4553, 4556
FriOI GRGCYC 1 cut(s) 3718
Fsp4HI GCNGC 7 cut(s) 1296, 1299, 1797, 2181, 3701, 4553, 4556
FspI TGCGCA 1 cut(s) 2016
GlaI GCGC 4 cut(s) 383, 1446, 2016, 4536
GluI GCNGC 7 cut(s) 1296, 1299, 1797, 2181, 3701, 4553, 4556
GsaI CCCAGC 1 cut(s) 1451
GsuI CTGGAG 6 cut(s) 558, 873, 1774, 3687, 3873, 4457
HaeII RGCGCY 1 cut(s) 1448
HaeIII GGCC 9 cut(s) 5, 2003, 2430, 3210, 3250, 3474, 3642, 4276, 4630
HapII CCGG 4 cut(s) 1551, 1618, 3050, 4254
HhaI GCGC 4 cut(s) 384, 1447, 2017, 4537
Hin6I GCGC 4 cut(s) 382, 1445, 2015, 4535
HinP1I GCGC 4 cut(s) 382, 1445, 2015, 4535
HincII GTYRAC 3 cut(s) 205, 769, 3961
HindII GTYRAC 3 cut(s) 205, 769, 3961
HindIII AAGCTT 2 cut(s) 1466, 3305
HinfI GANTC 9 cut(s) 110, 698, 1052, 1604, 2645, 3113, 4145, 4595, 4790
HpaII CCGG 4 cut(s) 1551, 1618, 3050, 4254
Hpy166II GTNNAC 7 cut(s) 205, 769, 1966, 2144, 2915, 3363, 3961
Hpy8I GTNNAC 7 cut(s) 205, 769, 1966, 2144, 2915, 3363, 3961
HpyCH4IV ACGT 2 cut(s) 1078, 3098
HpyF10VI GCNNNNNNNGC 5 cut(s) 356, 1213, 1295, 1664, 1837
HpySE526I ACGT 2 cut(s) 1078, 3098
HspAI GCGC 4 cut(s) 382, 1445, 2015, 4535
KpnI GGTACC 1 cut(s) 3008
Ksp22I TGATCA 5 cut(s) 2022, 2436, 2614, 3043, 3139
Lsp1109I GCAGC 7 cut(s) 1285, 1307, 1783, 2192, 3712, 4539, 4567
LweI GCATC 6 cut(s) 289, 1276, 1808, 3389, 3989, 4696
MabI ACCWGGT 1 cut(s) 1180
MaeII ACGT 2 cut(s) 1078, 3098
MbiI CCGCTC 2 cut(s) 1954, 4008
MflI RGATCY 6 cut(s) 1109, 2629, 2879, 2987, 4018, 4757
MhlI GDGCHC 5 cut(s) 556, 1822, 3328, 3718, 4141
MlsI TGGCCA 2 cut(s) 5, 3250
MluNI TGGCCA 2 cut(s) 5, 3250
MlyI GAGTC 3 cut(s) 1061, 4139, 4589
MmeI TCCRAC 2 cut(s) 220, 2105
Mox20I TGGCCA 2 cut(s) 5, 3250
Mph1103I ATGCAT 2 cut(s) 4028, 4591
MroXI GAANNNNTTC 4 cut(s) 369, 2421, 3268, 3655
MscI TGGCCA 2 cut(s) 5, 3250
MslI CAYNNNNRTG 5 cut(s) 192, 297, 1612, 3015, 3954
Msp20I TGGCCA 2 cut(s) 5, 3250
MspA1I CMGCKG 1 cut(s) 1298
MspI CCGG 4 cut(s) 1551, 1618, 3050, 4254
Mva1269I GAATGC 4 cut(s) 1391, 2756, 3182, 3620
MvnI CGCG 1 cut(s) 3030
MwoI GCNNNNNNNGC 5 cut(s) 356, 1213, 1295, 1664, 1837
NciI CCSGG 2 cut(s) 1619, 3051
NdeI CATATG 4 cut(s) 988, 3853, 4070, 4459
NlaIV GGNNCC 7 cut(s) 270, 1500, 3006, 3376, 3490, 4443, 4487
NmeAIII GCCGAG 1 cut(s) 4577
NmuCI GTSAC 8 cut(s) 73, 1019, 1546, 2405, 2451, 3539, 3820, 4064
NsbI TGCGCA 1 cut(s) 2016
NsiI ATGCAT 2 cut(s) 4028, 4591
NspI RCATGY 4 cut(s) 354, 2965, 4030, 4237
PaeI GCATGC 1 cut(s) 354
PaeR7I CTCGAG 1 cut(s) 2605
PagI TCATGA 3 cut(s) 601, 3513, 4720
PaqCI CACCTGC 1 cut(s) 2008
PceI AGGCCT 1 cut(s) 4276
PciI ACATGT 1 cut(s) 4233
PctI GAATGC 4 cut(s) 1391, 2756, 3182, 3620
PdmI GAANNNNTTC 4 cut(s) 369, 2421, 3268, 3655
PfeI GAWTC 6 cut(s) 110, 698, 1604, 2645, 3113, 4790
PflMI CCANNNNNTGG 1 cut(s) 2742
PfoI TCCNGGA 2 cut(s) 3664, 3889
PinAI ACCGGT 2 cut(s) 1550, 4253
PkrI GCNGC 7 cut(s) 1297, 1300, 1798, 2182, 3702, 4554, 4557
PleI GAGTC 3 cut(s) 1060, 4139, 4589
PpsI GAGTC 3 cut(s) 1060, 4139, 4589
PpuMI RGGWCCY 1 cut(s) 4485
PscI ACATGT 1 cut(s) 4233
Psp1406I AACGTT 1 cut(s) 3098
Psp5II RGGWCCY 1 cut(s) 4485
PspEI GGTNACC 3 cut(s) 1546, 2451, 3539
PspFI CCCAGC 1 cut(s) 1447
PspN4I GGNNCC 7 cut(s) 270, 1500, 3006, 3376, 3490, 4443, 4487
PspPI GGNCC 9 cut(s) 1553, 2001, 2212, 2270, 2325, 2915, 3488, 4485, 4628
PspPPI RGGWCCY 1 cut(s) 4485
PstI CTGCAG 2 cut(s) 1564, 4557
PstNI CAGNNNCTG 3 cut(s) 784, 2226, 4337
PsuI RGATCY 6 cut(s) 1109, 2629, 2879, 2987, 4018, 4757
PvuII CAGCTG 1 cut(s) 1298
RseI CAYNNNNRTG 5 cut(s) 192, 297, 1612, 3015, 3954
SalI GTCGAC 2 cut(s) 203, 767
SatI GCNGC 7 cut(s) 1296, 1299, 1797, 2181, 3701, 4553, 4556
Sau96I GGNCC 9 cut(s) 1553, 2001, 2212, 2270, 2325, 2915, 3488, 4485, 4628
ScaI AGTACT 2 cut(s) 164, 4117
SchI GAGTC 3 cut(s) 1061, 4139, 4589
SduI GDGCHC 5 cut(s) 556, 1822, 3328, 3718, 4141
SexAI ACCWGGT 1 cut(s) 1180
SfaNI GCATC 6 cut(s) 289, 1276, 1808, 3389, 3989, 4696
SfcI CTRYAG 9 cut(s) 501, 1221, 1435, 1560, 1597, 2316, 3200, 4119, 4553
Sfr274I CTCGAG 1 cut(s) 2605
SinI GGWCC 7 cut(s) 1553, 2212, 2270, 2325, 2915, 3488, 4485
SlaI CTCGAG 1 cut(s) 2605
SmiMI CAYNNNNRTG 5 cut(s) 192, 297, 1612, 3015, 3954
SmlI CTYRAG 1 cut(s) 2605
SmoI CTYRAG 1 cut(s) 2605
SpeI ACTAGT 2 cut(s) 1168, 3404
SphI GCATGC 1 cut(s) 354
SseBI AGGCCT 1 cut(s) 4276
SsiI CCGC 7 cut(s) 1527, 1954, 2625, 3378, 4006, 4049, 4101
SspI AATATT 1 cut(s) 761
StuI AGGCCT 1 cut(s) 4276
StyI CCWWGG 4 cut(s) 538, 2736, 3734, 3825
TaiI ACGT 2 cut(s) 1081, 3101
TaqII GACCGA 1 cut(s) 2287
TatI WGTACW 6 cut(s) 162, 1989, 2141, 2380, 3507, 4115
TfiI GAWTC 6 cut(s) 110, 698, 1604, 2645, 3113, 4790
TseFI GTSAC 8 cut(s) 73, 1019, 1546, 2405, 2451, 3539, 3820, 4064
TseI GCWGC 7 cut(s) 1295, 1298, 1796, 2180, 3700, 4552, 4555
Tsp45I GTSAC 8 cut(s) 73, 1019, 1546, 2405, 2451, 3539, 3820, 4064
TspGWI ACGGA 4 cut(s) 3111, 3666, 3855, 4528
Van91I CCANNNNNTGG 1 cut(s) 2742
VpaK11BI GGWCC 7 cut(s) 1553, 2212, 2270, 2325, 2915, 3488, 4485
XceI RCATGY 4 cut(s) 354, 2965, 4030, 4237
XcmI CCANNNNNNNNNTGG 2 cut(s) 1258, 3482
XhoI CTCGAG 1 cut(s) 2605
XmaJI CCTAGG 1 cut(s) 538
XmiI GTMKAC 3 cut(s) 204, 768, 1965
XmnI GAANNNNTTC 4 cut(s) 369, 2421, 3268, 3655
ZrmI AGTACT 2 cut(s) 164, 4117
Zsp2I ATGCAT 2 cut(s) 4028, 4591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.