pycom111g03460

pleiotropic drug resistance protein 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0000472
Physical Location & Seq
Reverse (-)
2120204 .. 2125984
5781 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g03460.6

Sequence Viewer

Length: 3810 bp
ATGCAGGCTGACTCTATTGTCCGAGGGGAAATTACCTACAATGGTTATAAGTTCAATGAGTTTGTTCCTCAGAAGACATCAGCTTATATAAGCCAATATGACCTGCACATTTCTGAGTTGACTGTGAGGGAAGCACTCGACTTCTCAGCGCGTTGTCAGGGTATTGGTAACCGAGCAGATATAATGAAAGAAGTCAGCAGAAGGGAGAAGCAATCAGGAATTGTCCCAGAGCCAGACATTGACACTTATATGAAGGCCATTTCAATCGAAGGGTTGAAAAACTCTCTCCAAACAGACTATATAATGAAGATTCTTGGGTTAGATATTTGCGCTGATACAATCGTAGGCGATGCAATGCAACGAGGGATTTCCGGTGGTCAAAAGAAAAGACTGACAACAGGGGAAATGATGATTGGTCCGGCAAGAGCTTTCTTTATGGATGAGATATCAACAGGCTTAGACAGTTCCACTACTTTTCAGATTGTTACTTGTTTGCAGCAATTTACACACGTAACAGAATCCACCATTTTGGTATCACTACTTCAGCCAACACCAGAGACATTTTCTCTCTTCGATGACATTATTTTAATGGCAGAAGGAAAAATTGTATATCACGGGCCTTGCAAAGACGTAGCAGAGTTCTTTGAGCACTGTGGTTTTCGTAGCCCCCCACGAAAAGGCATTTCTGACTTCCTACAAGAGGTGGTTTCTCAAAAGGATCAAGCAAAATATTGGTACCATGAAGGCCAACCCTATAGCTATGTTAGTGTTGACATGTTTGTAAATATGTCCAAGGACTTTCATGCTGGAAAGAAGCTAGATGAGGAACTTTGCAAGCCTTTCGACAAATCTGAGCACCACAAAATTGCTTTATCATTCAACTTCTACTCATTAACAAAATGGGAACTATTAAAAGCATGCACGGCTAGAGAATGGCTTCTTATGAAACGTAACTCATTTGTTCATGTATTCAAGTCGGTGCAGCTTGTAATCGTTGCGCTGGTCACAATGACAGTGTTTTTACGCACATGGATGGATATTGACGAGGTGCATGCAATGTACTACATGGGTTCTTTGTTTTATGCTCTCATTAGACTGATGACCAATGGGGCTGCAGAGCTCTCAATGACTGTCTCTAGACTTGCCGTCTTCTACAAGCAAAGAGATTTCTATTTTTATCCTGCTTGGGCGTATTCCATTCCAGCTATCATTTTAAAGATTCCATTTTCATTGTTGGATGCTTTTCTTTGGACATCTTTGACTTATTACGTCATCGGTTACAGTCCTGAGCCTGACAGGTTCTTCAAGCAGCTCCTTATTTTATTCCTTATGCATCAAGTATCAATATCGTTGTTTCGTTTGATTGCCTCATTGGTTCGGACTCCTTCAGTAGCAGCAACCATCGCTCTCTTCACTTTAATTGTAATGTTTCTGTTTGGCGGCTTTGTGATTCCGAGATCTTCTCTGCCTGCTTGGTTGGAATGGGGCTTTTGGATTTCGCCTCTAACATTTGGAGAAATAGGTGTTTCAATAAATGAATTCCATGCTCCAAGGTGGAAAAAGGTGAAGGAACAAATTTTATCTTCCAATGTCACTATAGGCCAGCAAGCACTAGAAAGCCGTGGTCTAAATTTCGATGACAAGTTTTATTGGATATCAGTAGGAGCTTTACTTGGATTTTGGATGGTTTTTAACCTTGGTTTCACCTTTGCACTCAGTTTTTTGAAATCTCCTGGAAGTTCTCGAAAAATCATCTCTCACGCAAGGTTCTCTAAGCTTAAGGGAAAAGGAAATTTAGATGCTTCCTTCCAAGAAAAAGAATTAACTTGTGTAGATACTCACACAACTTCTGCAGAACCTATAAGAATGGTCTTACCTTTTGAGCCCATAAGCATATCGTTTGAAAAAGTGCAATATTTTGTTGATACACCAAAGAAATTAAAAGATCAAGGTTTTTCTCCAAAAAGACTACAGCTTCTTCAAGATGTAACCGGTGCATTTAGACCTGGAGTTCTTACAGCTTTAATGGGAGTTAGTGGAGCTGGAAAAACAACACTGATGGATGTCCTTTCAGGAAGAAAAACTGGTGGAACCATCGAAGGAGACATCAGAATTGGAGGGTACCCTAAAGTCCAAGAAACATATGCCAGAATATCCGGTTATTGTGAGCAAAGTGACGTACACTCTCCACATATTACAGTCGAAGAATCAATAGCATACTCAGCTTGGTTGCGCTTGCCCGTCCAGATTGATAGGCCTACTAGATCTGTAAGTTATTTTTTAAACTTACAAGGCATGTATGATCCATCTATTTCTCAGCAATTTGTGAAAGAAGTTCTTCAAATGATAGAGCTTGACGAAGTCAAAGATGAATTAGTTGGCTCGCCTGGTGGTAGTGGGATATCGACAGAGCAACGAAAAAGACTTACAATAGCGGTAGAGCTTGTTTCAAATCCATCCATAATATTTATGGACGAACCAACCTCGGGTTTAGATGCTAGAGCAGCTGCTATAGTCATGAGAGTTGTGAAAAATATTGTTAGCACAAGGAGGACAATTGTATGCACTATTCACCAGCCAAGTATTGATATCTTCGAAGCATTCGACGAGCTTATTCTGATGAAAAGAGGAGGACAAGTAATATACAACGGGGAGCTGGGTCGAAATTCAAGTAAGCTTATCGAATACTTTGAGGCTATTCCCGGTGCTCCAAAAATCAAAGAGAATTACAACCCAGCAACGTGGATGTTAGAGGTCACCAATTCTTCTGCAGAAGCTCAACTTGCCATAGATTTTTCTCATCTCTACAAAGACTCACACCTATGCCAGAGGAACAACCAACTAGTTAGAGATCTCCAAATTCCAGAACAAGGCTCAGAGGAACTACATTTCTCTACTCGCTTTCCGCGAAATGGGTGGGAGCAATTCAAAGCATGCTTATGGAAGCGGCATCTCACCTACTGGAGAAGTCCGAGATACAACCTGGGGAGATTGATTTTTACTGCAGCATGTTCTTTGTTATTTGGAGCACTTCTTTGGCAGAAGGGACAGAAAATAGATGGTGAACAAGATTTCTTCAACATTCTAGGAGCAATGTTCGTTTTACTTCAATGCATGGGAATCGGCAACTGTTCCTCCGTTTTGCCATTCGTAGCTACTGAGCGCAGTATTGTATACCGAGAAAGGTTTGCTGGAATGTACTCTTCATGGGCATATTCGTTTTCACAGTTGGATAATTTTACTAAAACTCCTATATTTTTCAGGTCGATCGTGGAAATTCCATATATAACGCTACAAGCGGCTTTATTTTCGACGATTACATATCCGGCTATAAATTTCCACTGGTCACTCTACAAAGCATTTTGGTATTTCTATACTATGTTTTGTACATTGCTCTGGTTCAACTTCTTCGGTATGCTGCTTGTTTCTTTGACCCCAACTTTTCAAGTGGCTTCAGTATTGTCAAGTTTCTGCTACACTATGTTCAATTTGTTCTCAGGATTCCTCATACCTGGACCAGTAAGCAAACTCTTATCTTTACTTAAAATTCCTGCATGGTGGATTTGGGGTTACTGGATTTGCCCTCTAGCCTGGTCCTTAAAAGGTGTTCTCACTTCGCAGTACGGCGATCTGGAGAAGGAAATAGTAGTACGTGGAGAGCAAAAGGCAATTGGCGCTTTCTTAGAAAGTATTTACGGGTATAACTACAACGATCTAGGAGTTGTAGCAGCTGTTCTTTTTGCATTTCCACTAGTCTTTGCATTAGTTTTTGCAGGTGCAACGGAAAAATTAAACTTTCAGAGGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1270

Amino Acids

144.18

Weight (kDa)

7.93

Isoelectric Point (pI)

39.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000472)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15230 AT4G15230 AT4G15230 AT4G15230 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15236
fragaria_vesca FvH4_4g21360 FvH4_4g21360 FvH4_4g21390 FvH4_6g49431 FvH4_6g49450
malus_domestica MD09G1044300.v1.1 MD16G1137900.v1.1 MD16G1138000.v1.1
prunus_persica Prupe.1G106800_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.3G273300_v2.0.a1
pyrus_communis pycom111g03460 pycom16g12210
rosa_chinensis RchiOBHm_Chr2g0169331 RchiOBHm_Chr2g0169361 RchiOBHm_Chr2g0169371 RchiOBHm_Chr2g0169381 RchiOBHm_Chr2g0169411 RchiOBHm_Chr4g0427141 RchiOBHm_Chr4g0427321
rosa_laevigata RLG00000007252 RLG00000007258 RLG00000013974 RLG00000021856 RLG00000021857 RLG00000021858 RLG00000021859
rosa_multiflora Rmu_sc0000648.1_g000007 Rmu_sc0000648.1_g000009 Rmu_sc0003813.1_g000016 Rmu_sc0009165.1_g000008 Rmu_sc0022554.1_g000001
rosa_roxburghii Rroxscaffold_2G00082200 Rroxscaffold_2G00082230 Rroxscaffold_5G00368960 Rroxscaffold_5G00369010 Rroxscaffold_7G00200390
rosa_rugosa Rorug02G0539500 Rorug02G0539600 Rorug02G0539700 Rorug04G0216400 Rorug04G0216500 Rorug04G0216500 Rorug04G0216600
rosa_samantha Rh2AG610800 Rh2AG611000 Rh2AG611100 Rh2CG592800 Rh2CG593000 Rh2CG593200 Rh2DG634900 Rh2DG635100 Rh2DG635200 Rh2DG635400 Rh4AG272200 Rh4AG272900 Rh4DG275600 Rh4DG276000
rosa_wichuraiana Rw2G050640 Rw2G050660 Rw2G050670 Rw2G050680 Rw4G023610 Rw4G023680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 48
AarI CACCTGC 1 cut(s) 3764
AasI GACNNNNNNGTC 1 cut(s) 17
Acc36I ACCTGC 2 cut(s) 111, 3764
Acc65I GGTACC 2 cut(s) 735, 2121
AccB1I GGYRCC 2 cut(s) 735, 2121
AccI GTMKAC 1 cut(s) 3174
AccII CGCG 2 cut(s) 151, 2908
AciI CCGC 5 cut(s) 1440, 2435, 2906, 2947, 3299
AclWI GGATC 2 cut(s) 726, 2297
AcsI RAATTY 9 cut(s) 1538, 1575, 1630, 1792, 2665, 2859, 3276, 3334, 3546
AcuI CTGAAG 3 cut(s) 527, 1371, 3438
AfaI GTAC 8 cut(s) 737, 1061, 2123, 2181, 3200, 3388, 3623, 3651
AfiI CCNNNNNNNGG 5 cut(s) 677, 700, 2486, 2870, 2912
AflII CTTAAG 1 cut(s) 1778
AflIII ACRYGT 2 cut(s) 508, 774
AgeI ACCGGT 1 cut(s) 1991
AhdI GACNNNNNGTC 1 cut(s) 1145
AhlI ACTAGT 2 cut(s) 2842, 3751
AjnI CCWGG 6 cut(s) 1732, 2005, 2386, 2982, 3511, 3590
AjuI GAANNNNNNNTTGG 2 cut(s) 396, 428
Alw21I GWGCWC 5 cut(s) 651, 858, 1122, 2710, 3031
Alw26I GTCTC 3 cut(s) 551, 1138, 2097
AlwI GGATC 2 cut(s) 726, 2297
Ama87I CYCGRG 1 cut(s) 2485
AoxI GGCC 5 cut(s) 255, 617, 745, 1600, 2255
ApoI RAATTY 9 cut(s) 1538, 1575, 1630, 1792, 2665, 2859, 3276, 3334, 3546
AsiGI ACCGGT 1 cut(s) 1991
Asp700I GAANNNNTTC 2 cut(s) 936, 2337
Asp718I GGTACC 2 cut(s) 735, 2121
AspLEI GCGC 6 cut(s) 151, 332, 1000, 2235, 3165, 3677
AspS9I GGNCC 4 cut(s) 416, 617, 3515, 3594
AsuC2I CCSGG 1 cut(s) 2703
AsuHPI GGTGA 6 cut(s) 1576, 1696, 2564, 2749, 2947, 3074
AsuII TTCGAA 1 cut(s) 2595
AvaI CYCGRG 1 cut(s) 2485
AvaII GGWCC 3 cut(s) 416, 3515, 3594
BaeI ACNNNNGTAYC 2 cut(s) 327, 360
BanI GGYRCC 2 cut(s) 735, 2121
BanII GRGCYC 2 cut(s) 1122, 1887
BarI GAAGNNNNNNTAC 2 cut(s) 525, 557
BbsI GAAGAC 2 cut(s) 80, 1141
Bbv12I GWGCWC 5 cut(s) 651, 858, 1122, 2710, 3031
BccI CCATC 8 cut(s) 1027, 1409, 1678, 2053, 2102, 2314, 2464, 3053
BceAI ACGGC 4 cut(s) 939, 1130, 1605, 3640
BcgI CGANNNNNNTGC 6 cut(s) 823, 857, 1034, 1068, 3103, 3137
BciT130I CCWGG 6 cut(s) 1734, 2007, 2388, 2984, 3513, 3592
BcnI CCSGG 1 cut(s) 2703
BcoDI GTCTC 3 cut(s) 551, 1138, 2097
BcuI ACTAGT 2 cut(s) 2842, 3751
BfmI CTRYAG 8 cut(s) 754, 1113, 1596, 1851, 1970, 2511, 2769, 3003
BfoI RGCGCY 1 cut(s) 3678
BfrI CTTAAG 1 cut(s) 1778
BfuAI ACCTGC 2 cut(s) 111, 3764
BglII AGATCT 3 cut(s) 1457, 2264, 2851
Bme1390I CCNGG 7 cut(s) 1734, 2007, 2388, 2703, 2984, 3513, 3592
Bme18I GGWCC 3 cut(s) 416, 3515, 3594
BmeRI GACNNNNNGTC 1 cut(s) 1145
BmeT110I CYCGRG 1 cut(s) 2485
BmgT120I GGNCC 4 cut(s) 416, 617, 3515, 3594
BmiI GGNNCC 3 cut(s) 737, 2092, 2123
BmrFI CCNGG 7 cut(s) 1734, 2007, 2388, 2703, 2984, 3513, 3592
BmsI GCATC 6 cut(s) 340, 1228, 1342, 1789, 2485, 2959
BpiI GAAGAC 2 cut(s) 80, 1141
BplI GAGNNNNNCTC 2 cut(s) 1447, 1479
BpmI CTGGAG 3 cut(s) 2028, 2983, 3653
Bpu10I CCTNAGC 1 cut(s) 1287
Bpu14I TTCGAA 1 cut(s) 2595
BpuMI CCSGG 1 cut(s) 2703
BsaAI YACGTR 2 cut(s) 511, 3653
BsaJI CCNNGG 7 cut(s) 22, 792, 1550, 1621, 1696, 2484, 2983
BsaWI WCCGGW 3 cut(s) 371, 1991, 2156
BsaXI ACNNNNNCTCC 6 cut(s) 2622, 2652, 3119, 3149, 3232, 3262
Bsc4I CCNNNNNNNGG 5 cut(s) 677, 700, 2486, 2870, 2912
Bse118I RCCGGY 1 cut(s) 1991
Bse1I ACTGG 5 cut(s) 2089, 2966, 3347, 3518, 3578
Bse3DI GCAATG 4 cut(s) 360, 1062, 3099, 3389
BseBI CCWGG 6 cut(s) 1734, 2007, 2388, 2984, 3513, 3592
BseDI CCNNGG 7 cut(s) 22, 792, 1550, 1621, 1696, 2484, 2983
BseGI GGATG 7 cut(s) 445, 1038, 1243, 1689, 2068, 2456, 2751
BseLI CCNNNNNNNGG 5 cut(s) 677, 700, 2486, 2870, 2912
BseMI GCAATG 4 cut(s) 360, 1062, 3099, 3389
BseNI ACTGG 5 cut(s) 2089, 2966, 3347, 3518, 3578
BseRI GAGGAG 1 cut(s) 2643
BseYI CCCAGC 2 cut(s) 2656, 2734
BsgI GTGCAG 2 cut(s) 89, 1001
Bsh1236I CGCG 2 cut(s) 151, 2908
Bsh1285I CGRYCG 1 cut(s) 3270
BshFI GGCC 5 cut(s) 257, 619, 747, 1602, 2257
BshNI GGYRCC 2 cut(s) 735, 2121
BshTI ACCGGT 1 cut(s) 1991
BsiEI CGRYCG 1 cut(s) 3270
BsiHKAI GWGCWC 5 cut(s) 651, 858, 1122, 2710, 3031
BsiHKCI CYCGRG 1 cut(s) 2485
BsiSI CCGG 6 cut(s) 372, 419, 1992, 2157, 2703, 3326
BslFI GGGAC 2 cut(s) 209, 3060
BslI CCNNNNNNNGG 5 cut(s) 677, 700, 2486, 2870, 2912
BsmAI GTCTC 3 cut(s) 551, 1138, 2097
BsmFI GGGAC 2 cut(s) 209, 3060
BsmI GAATGC 1 cut(s) 2600
BsnI GGCC 5 cut(s) 257, 619, 747, 1602, 2257
BsoBI CYCGRG 1 cut(s) 2485
Bsp119I TTCGAA 1 cut(s) 2595
Bsp1286I GDGCHC 6 cut(s) 651, 858, 1122, 1887, 2710, 3031
Bsp1407I TGTACA 1 cut(s) 3386
Bsp143I GATC 9 cut(s) 718, 1457, 1945, 2264, 2302, 2851, 3267, 3628, 3712
BspACI CCGC 5 cut(s) 1440, 2435, 2906, 2947, 3299
BspANI GGCC 5 cut(s) 257, 619, 747, 1602, 2257
BspFNI CGCG 2 cut(s) 151, 2908
BspHI TCATGA 1 cut(s) 2517
BspLI GGNNCC 3 cut(s) 737, 2092, 2123
BspMAI CTGCAG 4 cut(s) 1117, 1855, 2773, 3007
BspMI ACCTGC 2 cut(s) 111, 3764
BspPI GGATC 2 cut(s) 726, 2297
BspT104I TTCGAA 1 cut(s) 2595
BspT107I GGYRCC 2 cut(s) 735, 2121
BspTI CTTAAG 1 cut(s) 1778
BsrDI GCAATG 4 cut(s) 360, 1062, 3099, 3389
BsrFI RCCGGY 1 cut(s) 1991
BsrGI TGTACA 1 cut(s) 3386
BsrI ACTGG 5 cut(s) 2089, 2966, 3347, 3518, 3578
BssAI RCCGGY 1 cut(s) 1991
BssECI CCNNGG 7 cut(s) 22, 792, 1550, 1621, 1696, 2484, 2983
BssMI GATC 9 cut(s) 718, 1457, 1945, 2264, 2302, 2851, 3267, 3628, 3712
BssNAI GTATAC 1 cut(s) 3175
BssT1I CCWWGG 3 cut(s) 792, 1550, 1696
Bst1107I GTATAC 1 cut(s) 3175
Bst2UI CCWGG 6 cut(s) 1734, 2007, 2388, 2984, 3513, 3592
Bst4CI ACNGT 9 cut(s) 124, 464, 653, 1015, 1132, 1283, 2200, 3131, 3228
Bst6I CTCTTC 3 cut(s) 575, 1415, 3208
BstAFI CTTAAG 1 cut(s) 1778
BstAUI TGTACA 1 cut(s) 3386
BstBAI YACGTR 2 cut(s) 511, 3653
BstBI TTCGAA 1 cut(s) 2595
BstDSI CCRYGG 1 cut(s) 1621
BstEII GGTNACC 2 cut(s) 167, 2755
BstENI CCTNNNNNAGG 1 cut(s) 698
BstF5I GGATG 7 cut(s) 445, 1038, 1243, 1689, 2068, 2456, 2751
BstFNI CGCG 2 cut(s) 151, 2908
BstH2I RGCGCY 1 cut(s) 3678
BstHHI GCGC 6 cut(s) 151, 332, 1000, 2235, 3165, 3677
BstKTI GATC 9 cut(s) 721, 1460, 1948, 2267, 2305, 2854, 3270, 3631, 3715
BstMAI GTCTC 3 cut(s) 551, 1138, 2097
BstMBI GATC 9 cut(s) 718, 1457, 1945, 2264, 2302, 2851, 3267, 3628, 3712
BstMCI CGRYCG 1 cut(s) 3270
BstMWI GCNNNNNNNGC 6 cut(s) 923, 1403, 2222, 2504, 2783, 3674
BstNI CCWGG 6 cut(s) 1734, 2007, 2388, 2984, 3513, 3592
BstNSI RCATGY 6 cut(s) 778, 921, 1055, 2299, 2937, 3012
BstPI GGTNACC 2 cut(s) 167, 2755
BstSCI CCNGG 7 cut(s) 1732, 2005, 2386, 2701, 2982, 3511, 3590
BstSFI CTRYAG 8 cut(s) 754, 1113, 1596, 1851, 1970, 2511, 2769, 3003
BstUI CGCG 2 cut(s) 151, 2908
BstV2I GAAGAC 2 cut(s) 80, 1141
BstX2I RGATCY 3 cut(s) 1457, 2264, 2851
BstXI CCANNNNNNTGG 2 cut(s) 529, 2742
BstYI RGATCY 3 cut(s) 1457, 2264, 2851
BstZ17I GTATAC 1 cut(s) 3175
BsuRI GGCC 5 cut(s) 257, 619, 747, 1602, 2257
BtgI CCRYGG 1 cut(s) 1621
BtgZI GCGATG 2 cut(s) 363, 1387
BtsCI GGATG 7 cut(s) 445, 1038, 1243, 1689, 2068, 2456, 2751
BtsIMutI CAGTG 4 cut(s) 649, 1020, 2054, 3340
BveI ACCTGC 2 cut(s) 111, 3764
CciI TCATGA 1 cut(s) 2517
CfoI GCGC 6 cut(s) 151, 332, 1000, 2235, 3165, 3677
Cfr10I RCCGGY 1 cut(s) 1991
Cfr13I GGNCC 4 cut(s) 416, 617, 3515, 3594
Csp6I GTAC 8 cut(s) 736, 1060, 2122, 2180, 3199, 3387, 3622, 3650
CspAI ACCGGT 1 cut(s) 1991
CviQI GTAC 8 cut(s) 736, 1060, 2122, 2180, 3199, 3387, 3622, 3650
DpnI GATC 9 cut(s) 720, 1459, 1947, 2266, 2304, 2853, 3269, 3630, 3714
DpnII GATC 9 cut(s) 718, 1457, 1945, 2264, 2302, 2851, 3267, 3628, 3712
DraI TTTAAA 2 cut(s) 1215, 2283
DrdI GACNNNNNNGTC 1 cut(s) 17
DriI GACNNNNNGTC 1 cut(s) 1145
DseDI GACNNNNNNGTC 1 cut(s) 17
Eam1104I CTCTTC 3 cut(s) 575, 1415, 3208
Eam1105I GACNNNNNGTC 1 cut(s) 1145
EarI CTCTTC 3 cut(s) 575, 1415, 3208
Ecl136II GAGCTC 1 cut(s) 1120
Eco130I CCWWGG 3 cut(s) 792, 1550, 1696
Eco147I AGGCCT 1 cut(s) 2257
Eco24I GRGCYC 2 cut(s) 1122, 1887
Eco32I GATATC 4 cut(s) 447, 1656, 2403, 2590
Eco47I GGWCC 3 cut(s) 416, 3515, 3594
Eco53kI GAGCTC 1 cut(s) 1120
Eco57I CTGAAG 3 cut(s) 527, 1371, 3438
Eco88I CYCGRG 1 cut(s) 2485
Eco91I GGTNACC 2 cut(s) 167, 2755
EcoICRI GAGCTC 1 cut(s) 1120
EcoNI CCTNNNNNAGG 1 cut(s) 698
EcoO65I GGTNACC 2 cut(s) 167, 2755
EcoRI GAATTC 1 cut(s) 1538
EcoRII CCWGG 6 cut(s) 1732, 2005, 2386, 2982, 3511, 3590
EcoRV GATATC 4 cut(s) 447, 1656, 2403, 2590
EcoT14I CCWWGG 3 cut(s) 792, 1550, 1696
EcoT22I ATGCAT 2 cut(s) 1335, 3116
EcoT38I GRGCYC 2 cut(s) 1122, 1887
ErhI CCWWGG 3 cut(s) 792, 1550, 1696
FalI AAGNNNNNCTT 4 cut(s) 2322, 2354, 2766, 2798
FaqI GGGAC 2 cut(s) 209, 3060
FauNDI CATATG 1 cut(s) 2143
FblI GTMKAC 1 cut(s) 3174
FokI GGATG 7 cut(s) 452, 1045, 1250, 1696, 2075, 2443, 2758
FriOI GRGCYC 2 cut(s) 1122, 1887
GlaI GCGC 6 cut(s) 150, 331, 999, 2234, 3164, 3676
GsaI CCCAGC 2 cut(s) 2660, 2738
GsuI CTGGAG 3 cut(s) 2028, 2983, 3653
HaeII RGCGCY 1 cut(s) 3678
HaeIII GGCC 5 cut(s) 257, 619, 747, 1602, 2257
HapII CCGG 6 cut(s) 372, 419, 1992, 2157, 2703, 3326
HhaI GCGC 6 cut(s) 151, 332, 1000, 2235, 3165, 3677
Hin6I GCGC 6 cut(s) 149, 330, 998, 2233, 3163, 3675
HinP1I GCGC 6 cut(s) 149, 330, 998, 2233, 3163, 3675
HincII GTYRAC 2 cut(s) 120, 772
HindII GTYRAC 2 cut(s) 120, 772
HindIII AAGCTT 2 cut(s) 1775, 2675
HpaII CCGG 6 cut(s) 372, 419, 1992, 2157, 2703, 3326
HphI GGTGA 6 cut(s) 1576, 1696, 2564, 2749, 2947, 3074
Hpy166II GTNNAC 5 cut(s) 120, 772, 2182, 3065, 3175
Hpy8I GTNNAC 5 cut(s) 120, 772, 2182, 3065, 3175
Hpy99I CGWCG 2 cut(s) 2609, 3316
HpyCH4III ACNGT 9 cut(s) 124, 464, 653, 1015, 1132, 1283, 2200, 3131, 3228
HpyCH4IV ACGT 7 cut(s) 510, 630, 949, 1269, 2178, 2741, 3652
HpyF10VI GCNNNNNNNGC 6 cut(s) 923, 1403, 2222, 2504, 2783, 3674
HpySE526I ACGT 7 cut(s) 510, 630, 949, 1269, 2178, 2741, 3652
HspAI GCGC 6 cut(s) 149, 330, 998, 2233, 3163, 3675
KpnI GGTACC 2 cut(s) 739, 2125
Kzo9I GATC 9 cut(s) 718, 1457, 1945, 2264, 2302, 2851, 3267, 3628, 3712
LmnI GCTCC 9 cut(s) 1317, 1552, 1664, 2039, 2653, 2713, 2920, 3026, 3089
LweI GCATC 6 cut(s) 340, 1228, 1342, 1789, 2485, 2959
MaeII ACGT 7 cut(s) 510, 630, 949, 1269, 2178, 2741, 3652
MalI GATC 9 cut(s) 720, 1459, 1947, 2266, 2304, 2853, 3269, 3630, 3714
MboI GATC 9 cut(s) 718, 1457, 1945, 2264, 2302, 2851, 3267, 3628, 3712
MfeI CAATTG 2 cut(s) 2556, 3669
MflI RGATCY 3 cut(s) 1457, 2264, 2851
MhlI GDGCHC 6 cut(s) 651, 858, 1122, 1887, 2710, 3031
MlyI GAGTC 3 cut(s) 5, 1375, 2807
MmeI TCCRAC 3 cut(s) 1215, 1458, 3210
Mph1103I ATGCAT 2 cut(s) 1335, 3116
MroXI GAANNNNTTC 2 cut(s) 936, 2337
MslI CAYNNNNRTG 4 cut(s) 248, 1031, 2821, 2938
MspA1I CMGCKG 2 cut(s) 2507, 3731
MspCI CTTAAG 1 cut(s) 1778
MspI CCGG 6 cut(s) 372, 419, 1992, 2157, 2703, 3326
MspR9I CCNGG 7 cut(s) 1734, 2007, 2388, 2703, 2984, 3513, 3592
MunI CAATTG 2 cut(s) 2556, 3669
Mva1269I GAATGC 1 cut(s) 2600
MvaI CCWGG 6 cut(s) 1734, 2007, 2388, 2984, 3513, 3592
MvnI CGCG 2 cut(s) 151, 2908
MwoI GCNNNNNNNGC 6 cut(s) 923, 1403, 2222, 2504, 2783, 3674
NciI CCSGG 1 cut(s) 2703
NdeI CATATG 1 cut(s) 2143
NdeII GATC 9 cut(s) 718, 1457, 1945, 2264, 2302, 2851, 3267, 3628, 3712
NlaIV GGNNCC 3 cut(s) 737, 2092, 2123
NmuCI GTSAC 5 cut(s) 1003, 1591, 2174, 2755, 3345
NsiI ATGCAT 2 cut(s) 1335, 3116
NspI RCATGY 6 cut(s) 778, 921, 1055, 2299, 2937, 3012
NspV TTCGAA 1 cut(s) 2595
PaeI GCATGC 3 cut(s) 921, 1055, 2937
PagI TCATGA 1 cut(s) 2517
PaqCI CACCTGC 1 cut(s) 3764
PceI AGGCCT 1 cut(s) 2257
PciI ACATGT 1 cut(s) 774
PcsI WCGNNNNNNNCGW 2 cut(s) 2601, 2905
PctI GAATGC 1 cut(s) 2600
PdmI GAANNNNTTC 2 cut(s) 936, 2337
PfeI GAWTC 7 cut(s) 310, 518, 1219, 1450, 2207, 3120, 3501
PflFI GACNNNGTC 1 cut(s) 2360
PfoI TCCNGGA 1 cut(s) 1732
PinAI ACCGGT 1 cut(s) 1991
Ple19I CGATCG 1 cut(s) 3270
PleI GAGTC 3 cut(s) 5, 1375, 2807
PpsI GAGTC 3 cut(s) 5, 1375, 2807
Ppu21I YACGTR 2 cut(s) 511, 3653
PscI ACATGT 1 cut(s) 774
PsiI TTATAA 1 cut(s) 48
Psp124BI GAGCTC 1 cut(s) 1122
Psp6I CCWGG 6 cut(s) 1732, 2005, 2386, 2982, 3511, 3590
PspEI GGTNACC 2 cut(s) 167, 2755
PspFI CCCAGC 2 cut(s) 2656, 2734
PspGI CCWGG 6 cut(s) 1732, 2005, 2386, 2982, 3511, 3590
PspN4I GGNNCC 3 cut(s) 737, 2092, 2123
PspPI GGNCC 4 cut(s) 416, 617, 3515, 3594
PsrI GAACNNNNNNTAC 2 cut(s) 3717, 3749
PstI CTGCAG 4 cut(s) 1117, 1855, 2773, 3007
PsuI RGATCY 3 cut(s) 1457, 2264, 2851
PsyI GACNNNGTC 1 cut(s) 2360
PvuI CGATCG 1 cut(s) 3270
PvuII CAGCTG 2 cut(s) 2507, 3731
RsaI GTAC 8 cut(s) 737, 1061, 2123, 2181, 3200, 3388, 3623, 3651
RsaNI GTAC 8 cut(s) 736, 1060, 2122, 2180, 3199, 3387, 3622, 3650
RseI CAYNNNNRTG 4 cut(s) 248, 1031, 2821, 2938
SacI GAGCTC 1 cut(s) 1122
Sau3AI GATC 9 cut(s) 718, 1457, 1945, 2264, 2302, 2851, 3267, 3628, 3712
Sau96I GGNCC 4 cut(s) 416, 617, 3515, 3594
SchI GAGTC 3 cut(s) 5, 1375, 2807
ScrFI CCNGG 7 cut(s) 1734, 2007, 2388, 2703, 2984, 3513, 3592
SduI GDGCHC 6 cut(s) 651, 858, 1122, 1887, 2710, 3031
SfaNI GCATC 6 cut(s) 340, 1228, 1342, 1789, 2485, 2959
SfcI CTRYAG 8 cut(s) 754, 1113, 1596, 1851, 1970, 2511, 2769, 3003
SfuI TTCGAA 1 cut(s) 2595
SinI GGWCC 3 cut(s) 416, 3515, 3594
SmiMI CAYNNNNRTG 4 cut(s) 248, 1031, 2821, 2938
SmlI CTYRAG 1 cut(s) 1778
SmoI CTYRAG 1 cut(s) 1778
SpeI ACTAGT 2 cut(s) 2842, 3751
SphI GCATGC 3 cut(s) 921, 1055, 2937
SseBI AGGCCT 1 cut(s) 2257
SsiI CCGC 5 cut(s) 1440, 2435, 2906, 2947, 3299
SspI AATATT 4 cut(s) 731, 1916, 2466, 2536
SstI GAGCTC 1 cut(s) 1122
StuI AGGCCT 1 cut(s) 2257
StyD4I CCNGG 7 cut(s) 1732, 2005, 2386, 2701, 2982, 3511, 3590
StyI CCWWGG 3 cut(s) 792, 1550, 1696
TaaI ACNGT 9 cut(s) 124, 464, 653, 1015, 1132, 1283, 2200, 3131, 3228
TaiI ACGT 7 cut(s) 513, 633, 952, 1272, 2181, 2744, 3655
TatI WGTACW 3 cut(s) 1059, 3198, 3386
TauI GCSGC 3 cut(s) 1443, 2950, 3302
TfiI GAWTC 7 cut(s) 310, 518, 1219, 1450, 2207, 3120, 3501
TscAI CASTG 4 cut(s) 656, 1020, 2061, 3347
TseFI GTSAC 5 cut(s) 1003, 1591, 2174, 2755, 3345
Tsp45I GTSAC 5 cut(s) 1003, 1591, 2174, 2755, 3345
TspGWI ACGGA 2 cut(s) 3127, 3797
TspRI CASTG 4 cut(s) 656, 1020, 2061, 3347
Tth111I GACNNNGTC 1 cut(s) 2360
Vha464I CTTAAG 1 cut(s) 1778
VpaK11BI GGWCC 3 cut(s) 416, 3515, 3594
XagI CCTNNNNNAGG 1 cut(s) 698
XapI RAATTY 9 cut(s) 1538, 1575, 1630, 1792, 2665, 2859, 3276, 3334, 3546
XbaI TCTAGA 1 cut(s) 1136
XceI RCATGY 6 cut(s) 778, 921, 1055, 2299, 2937, 3012
XcmI CCANNNNNNNNNTGG 1 cut(s) 2467
XmiI GTMKAC 1 cut(s) 3174
XmnI GAANNNNTTC 2 cut(s) 936, 2337
Zsp2I ATGCAT 2 cut(s) 1335, 3116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.