RchiOBHm_Chr2g0169361

pleiotropic drug resistance protein 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000472
Physical Location & Seq
Forward (+)
83578358 .. 83589927
11570 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53695

Sequence Viewer

Length: 4173 bp
ATGGATTTGGCTGCAGAGATAGGAAGAAGTGTGAGGACTTCTTTTCATCAGCATGCATCAAGTTTCAGGAGCACTTCAGATGTCAGGTCCATGAAAGAGAATGATGACGATGAAGTTGAGTTGCGGTGGGCTGCCATTGAAAGATTGCCCACATTTGAACGTATTCGAACTTCACTCTTCGGTTGTAACAAGGAGGACGATGATCATGAAGGTAAGAGTAAGAGGGTAGTTGATGTTACAAAGCTAGAAGCCCTGGAACGTCGTGTGTTCATTGATAAACTTCTCAAGCAAATAGAAGGAGATAATCAATGCCTCTTACAGAAACTCAAAGAAAGAATAGACAGAGTGGGACTGCAAATGCCGACTGTGGAAGTACGATACAAAAATTTGTTTGTAGAAGCAGAATGTGAAGTAGTTCAAGGAAAGCCCCTCCCAACACTATGGAACACGCTCAAAAGCTTTCTCTCTGTCACGACACAGATAATTGGATGCAAGTCTGAAGCATACAATCTAGAAATTCTCAAAGATGTCAGTGGCATCATCAAGCCTTCAAGGATGACTCTATTGCTTGGTCCTCCAGGCTGTGGAAAAACTACCTTATTACAGGCACTTGCAGGAAAACTTAACCACCCTCTCAAGGTTCAAGGAGAAATAACCTACAATGGTTATAAGCTCAATGAGTTTGTTCCTCAGAAGACATCAGCTTACATAAGTCAATATGACTTGCATATATCTGAGATGACTGTGAGGGAAACACTGGATTTCTCATCTCGTTGCCAGGGCATTGGAAGCAGAGCTGATATTATGAAAGAAGTTGCTAGAAGGGAGAAGCAAGCTGGTATTGTCCCAGAACCAGATATTGACACTTATATGAAGGCCATTTCAATTGAAGGGTTGAAAAAATCTCTAATGACAGACTATATAATAAAGATCCTTGGGCTGGACATTTGTGCTGAGACAATCACAGGGGACCCAATGCAAAGAGGAATTTCGGGTGGTCAAAAGAAAAGGCTAACAACAGGTGAGATGATGATTGGCTCAGCAAGAGCTTTCTTCATGGATGAGATATCAACAGGACTAGACAGCTCCACTACATTTCAGATTGTAACTTGTCTGCAGCAATTGACTCACGTAACAGAATCGTCCATTTTGGTTTCACTTCTTCAGCCAACGCCAGAGACCTTTCATCTGTTTGATGACATTATCTTGATGGCTGAAGGAAAAGTTGTATACCATGGGCCTAGTGACAATGTTGTGGAGTTCTTTCAGAAGTGTGGTTTCCTTAGCCCACCACGAAAAGGCATTTCTGACTTCCTCCAAGAGGTGGTTTCTCAAAAGGATCAAGCAAAGTACTGGTGCCATAAAGACCAACCCTACAGTTATGTTAGTGTTGATGAGTTCGTAAGTATGTTTAAGGACTTCCATGTCGGAAAGAAGCTTGAAGAGGAACTTTCCAAGCCTTTTGAGCAATCAGAGTGCCATAAGCATGCTTTGTCATACAACATTTACTCACTGAGAAAATGGGAACTATTCAAAGCATGCCTTTCTAGAGAATGGCTTCTCATGAAGCGAAACTCTTTTGTTCATGTATTCCAATCAGTACAGCTTGTGGTCATTGCACTAGTAACAATGACAATGTTTCTACGTACATGGATGAAAGTCAACGAGATCTATGCAAAGTACTACATGAGTGCTTTGTTTTATGCTCTAATTAGATTTCTATGCAATGCAAGTTCAGAGATATCAATGACTTCCTCTAGACTTGCAGTCTTCTATAAGCAAAGAGATGTCTACTTTTACCCTGCATGGGCTTATTCTATTCCGGCTGCCATCCTGAAGATTCCATTTTCATTGCTAGATGCATTTCTTTGGACAGCCCTTACTTATTATGTGATTGGTTACAGTCCTGAACCTGAAAGGTTCTTCAGGCAATTTATTCTTCTGTTCCTCGTGCATCAAGTAGCAATATCACTATTTCGTTTGATTGCATCCTTGGTCCGAAATCCTTCTGTTGCAGGATCTGTCGGCCTTTTATCTTTAATAGTAATGTTTCTATTTGGAGGCTTCATAATTCCAAAATCTTCTCTACCTGCATGGTTGGAATGGGGATTTTGGGTTTCTCCATTGGCCTTTGCAGAACTAGGTGTTTCAATAAATGAATTTCATGCTCCAAGGTGGCAAAAGGTTTTATCTTCCAATGTCACTATTGGCCAGCAAGCTCTAATTAATCGTGGTCTAAACTTCAATGATTACTTCTATTGGATATCCATAGGAGCTTTAATTGGATTTTGGATGGTTTTCAACCTGGGGTTCACATGGACATTGAGTTATTCAAAGTGCATGGTGTTGCCTTTTGAGCCCATTACTATATCATTTGAGAAACTGCAATACTTTATTGATACACCCCAGAAACTGAGAGAGCAAGGTTTCCCACCAAATCGACTACAGCTTCTTCAAGATATAACTGGTGCATTTAGACCTGGCGTTCTTACAGCACTGATGGGTGTTAGTGGAGCAGGAAAGACAACACTGATGGATGTTCTTTCTGGAAGGAAAACTGGTGGACTTATTGAAGGAGACATTAGAATTAGAGGACACCCAAAAGTCCAAGAAACATATGCCAGAATATCTGGGTATTGCGAGCAAAGTGATATTCATTCTCCAGAGCTTACAGTAGAAGAGTCAGTAGCATACTCAGCTTGGTTACGCTTGCCATCCCAGATTGCTAGAAATACTAGAGCTGAGTTTGTGAAAGAAGTACTTCATATGATAGAGCTTGATGAAATCAAAGATGAGTTAGTTGGCATACCAGGTGGTAGTGGTATATCGACTGAACAGCGTAAAAGGCTAACAATAGCAGTAGAGCTTGTTTCAAATCCATCCGTTATATTTATGGATGAACCCACATCTGGTTTAGATGCCAGAGCAGCTGCAATAGTTATGAGAGTTGTGAAAAACATTGTCAGTACAAGAAGGACTGTTGTTTGCACTATTCATCAGCCAAGTATTGACATATTTGAAGCATTCGATGAGCTTCTTTTGATGAAAAGAGGAGGACAAGTAATATATTGTGGAGAGCTGGGTCAAGATTCAAGTAAGCTTATCCAATACTTTGAGGGTATTTCTGGGGTTCCAAAAATCAAAGATAATTACAACCCAGCAACATGGATGTTAGAAGTCACAAGTCCTTCTGCAGAAGCCAAACTTGCCTTAGATTTTGCTCAGCTTTATAGAGAATCACATCTATGCCAGAAGAACAATGACCTAGTTAGAGAACACAGCTTACCAGCACAAGGCTCAAAGGGATTGGATTTTCCTACTCGTTTCCCACAAAATGGATGGGAGCAATTTAAAGCATGTCTGTGGAAGCGACATCTCTCCTACTGGAGAAATCCAAAATATAACCTGGGGCGATTGTTAATCACTGCTGCATCCTCTTTGATATTTGGAGCACTTCTTTGGCAGAAAGGACAGAAAATAAATGGTGAACAGGAGTTCTTCAGCATTCTAGGATCAATATTTATTTTACAACAAAACATGGGAATTGGTAATTGTTCCTCTGTTTTACCATTTGTCGCTTCTGAGCGCAATGTTGTATATCGAGAAAGGTTTGCTGGAATGTACTCCTCATGGGCATATTCTTTATCACAGGTGATCATTGAACTTCCTTATGTATTCTTTCAAGCAGTTTTGTTTTCGACCATTACATATCCATCGATTGGTTTCCACTGGTCACTTTACAAAGTATTCTGGTATGTGTATGCTATGTTTTTCACGCTGCTCTACTTCACTTACTTCGGTATATTGGTTGCTTCTGTGACCCCGACTTTCCAAGTAGCTTCAGTATTAGCAAGTTTCTGCTACACCATGCTCAATCTGTTCTCGGGATACCTAATACCTCAACCAAACATTCCTAAATGGTGGGTTTGGGGCTACTATATTTGCCCTTTATCGTGGTCCTTAAAAGGTATTCTCACTTCACAGTACGGAGATCTAAACACGGAAATAGTACTCCGCGGGGAAGAAAAGACGATCAGTGCCTTCTTAGACAGCATCTATGGATACAACTATGATGATCTAGGAATTGTAGGAATTTTTCTTCTTGCATATCCACTAGTTTTCGCATTTGCGTTTGCACTTGCTACTGAAAAACTAAACTTCCAGAGGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1390

Amino Acids

158.06

Weight (kDa)

7.86

Isoelectric Point (pI)

40.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 174 - 355 1.9e-15 ABC transporter
ABC2_membrane PF01061 510 - 721 2.3e-41 ABC-2 type transporter
PDR_assoc PF08370 726 - 779 1.5e-15 Plant PDR ABC transporter associated
ABC_tran PF00005 818 - 970 5.3e-20 ABC transporter
ABC2_membrane PF01061 1115 - 1329 2.5e-56 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000472)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15215 AT4G15230 AT4G15230 AT4G15230 AT4G15230 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15233 AT4G15236
fragaria_vesca FvH4_4g21360 FvH4_4g21360 FvH4_4g21390 FvH4_6g49431 FvH4_6g49450
malus_domestica MD09G1044300.v1.1 MD16G1137900.v1.1 MD16G1138000.v1.1
prunus_persica Prupe.1G106800_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.1G106900_v2.0.a1 Prupe.3G273300_v2.0.a1
pyrus_communis pycom111g03460 pycom16g12210
rosa_chinensis RchiOBHm_Chr2g0169331 RchiOBHm_Chr2g0169361 RchiOBHm_Chr2g0169371 RchiOBHm_Chr2g0169381 RchiOBHm_Chr2g0169411 RchiOBHm_Chr4g0427141 RchiOBHm_Chr4g0427321
rosa_laevigata RLG00000007252 RLG00000007258 RLG00000013974 RLG00000021856 RLG00000021857 RLG00000021858 RLG00000021859
rosa_multiflora Rmu_sc0000648.1_g000007 Rmu_sc0000648.1_g000009 Rmu_sc0003813.1_g000016 Rmu_sc0009165.1_g000008 Rmu_sc0022554.1_g000001
rosa_roxburghii Rroxscaffold_2G00082200 Rroxscaffold_2G00082230 Rroxscaffold_5G00368960 Rroxscaffold_5G00369010 Rroxscaffold_7G00200390
rosa_rugosa Rorug02G0539500 Rorug02G0539600 Rorug02G0539700 Rorug04G0216400 Rorug04G0216500 Rorug04G0216500 Rorug04G0216600
rosa_samantha Rh2AG610800 Rh2AG611000 Rh2AG611100 Rh2CG592800 Rh2CG593000 Rh2CG593200 Rh2DG634900 Rh2DG635100 Rh2DG635200 Rh2DG635400 Rh4AG272200 Rh4AG272900 Rh4DG275600 Rh4DG276000
rosa_wichuraiana Rw2G050640 Rw2G050660 Rw2G050670 Rw2G050680 Rw4G023610 Rw4G023680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 669
AasI GACNNNNNNGTC 1 cut(s) 1424
Acc36I ACCTGC 1 cut(s) 2098
AccB1I GGYRCC 1 cut(s) 1356
AccB7I CCANNNNNTGG 4 cut(s) 584, 1324, 3335, 3719
AccI GTMKAC 2 cut(s) 1230, 1791
AccII CGCG 1 cut(s) 4017
AciI CCGC 3 cut(s) 124, 4015, 4017
AclWI GGATC 4 cut(s) 925, 1347, 2026, 3520
AcoI YGGCCR 1 cut(s) 2209
AcsI RAATTY 5 cut(s) 385, 516, 987, 2159, 4092
AcuI CTGAAG 8 cut(s) 60, 519, 1148, 1236, 1856, 1909, 3484, 3825
AhdI GACNNNNNGTC 1 cut(s) 1766
AhlI ACTAGT 2 cut(s) 1621, 4114
AjnI CCWGG 7 cut(s) 252, 577, 777, 2304, 2479, 2809, 3405
Alw21I GWGCWC 2 cut(s) 74, 3454
Alw26I GTCTC 3 cut(s) 950, 1172, 2571
AlwI GGATC 4 cut(s) 925, 1347, 2026, 3520
AlwNI CAGNNNCTG 2 cut(s) 2021, 2413
Ama87I CYCGRG 1 cut(s) 3883
AoxI GGCC 5 cut(s) 876, 1238, 2026, 2127, 2209
ApeKI GCWGC 8 cut(s) 11, 131, 1117, 1826, 2927, 2930, 3428, 3778
ApoI RAATTY 5 cut(s) 385, 516, 987, 2159, 4092
AseI ATTAAT 1 cut(s) 2226
Asp700I GAANNNNTTC 5 cut(s) 162, 414, 1557, 2005, 2760
AspLEI GCGC 1 cut(s) 3588
AspS9I GGNCC 6 cut(s) 87, 572, 970, 1238, 1996, 3957
AsuHPI GGTGA 3 cut(s) 1034, 3497, 3664
AsuII TTCGAA 1 cut(s) 166
AvaI CYCGRG 1 cut(s) 3883
AvaII GGWCC 5 cut(s) 87, 572, 970, 1996, 3957
BalI TGGCCA 1 cut(s) 2211
BanI GGYRCC 1 cut(s) 1356
BanII GRGCYC 1 cut(s) 2361
BarI GAAGNNNNNNTAC 4 cut(s) 154, 186, 3961, 3993
BauI CACGAG 1 cut(s) 1949
BbsI GAAGAC 2 cut(s) 701, 1762
Bbv12I GWGCWC 2 cut(s) 74, 3454
BbvI GCAGC 7 cut(s) 118, 1129, 1813, 2917, 2939, 3415, 3765
BccI CCATC 9 cut(s) 1204, 1838, 2287, 2494, 2527, 2722, 2887, 3333, 3721
BcgI CGANNNNNNTGC 2 cut(s) 1655, 1689
BciT130I CCWGG 7 cut(s) 254, 579, 779, 2306, 2481, 2811, 3407
BciVI GTATCC 2 cut(s) 3881, 4055
BclI TGATCA 2 cut(s) 202, 3654
BcoDI GTCTC 3 cut(s) 950, 1172, 2571
BcuI ACTAGT 2 cut(s) 1621, 4114
BfmI CTRYAG 5 cut(s) 12, 1115, 1375, 2444, 3192
BfuAI ACCTGC 1 cut(s) 2098
BfuI GTATCC 2 cut(s) 3881, 4055
BglII AGATCT 2 cut(s) 1668, 3991
BisI GCNGC 8 cut(s) 12, 132, 1118, 1827, 2928, 2931, 3429, 3779
BlpI GCTNAGC 2 cut(s) 1039, 3222
BlsI GCNGC 8 cut(s) 13, 133, 1119, 1828, 2929, 2932, 3430, 3780
BmcAI AGTACT 4 cut(s) 1352, 1682, 2760, 4011
Bme1390I CCNGG 7 cut(s) 254, 579, 779, 2306, 2481, 2811, 3407
Bme18I GGWCC 5 cut(s) 87, 572, 970, 1996, 3957
BmeRI GACNNNNNGTC 1 cut(s) 1766
BmeT110I CYCGRG 1 cut(s) 3883
BmgT120I GGNCC 6 cut(s) 87, 572, 970, 1238, 1996, 3957
BmiI GGNNCC 4 cut(s) 971, 972, 1358, 3132
BmrFI CCNGG 7 cut(s) 254, 579, 779, 2306, 2481, 2811, 3407
BmsI GCATC 9 cut(s) 65, 479, 546, 1849, 1963, 1997, 2908, 3440, 4062
BpiI GAAGAC 2 cut(s) 701, 1762
BpmI CTGGAG 3 cut(s) 561, 2646, 3406
Bpu10I CCTNAGC 1 cut(s) 1283
Bpu1102I GCTNAGC 2 cut(s) 1039, 3222
Bpu14I TTCGAA 1 cut(s) 166
BpuEI CTTGAG 2 cut(s) 269, 620
Bsa29I ATCGAT 1 cut(s) 3716
BsaAI YACGTR 2 cut(s) 1132, 1646
BsaI GGTCTC 1 cut(s) 1172
BsaJI CCNNGG 9 cut(s) 252, 778, 934, 1234, 1992, 2171, 2305, 3406, 4015
BsaXI ACNNNNNCTCC 2 cut(s) 3045, 3075
Bse1I ACTGG 6 cut(s) 762, 1358, 2470, 2563, 3389, 3734
Bse3DI GCAATG 4 cut(s) 1614, 1732, 1850, 3595
BseBI CCWGG 7 cut(s) 254, 579, 779, 2306, 2481, 2811, 3407
BseCI ATCGAT 1 cut(s) 3716
BseDI CCNNGG 9 cut(s) 252, 778, 934, 1234, 1992, 2171, 2305, 3406, 4015
BseMI GCAATG 4 cut(s) 1614, 1732, 1850, 3595
BseNI ACTGG 6 cut(s) 762, 1358, 2470, 2563, 3389, 3734
BseRI GAGGAG 2 cut(s) 3066, 3616
BseXI GCAGC 7 cut(s) 118, 1129, 1813, 2917, 2939, 3415, 3765
BseYI CCCAGC 2 cut(s) 3079, 3157
Bsh1236I CGCG 1 cut(s) 4017
BshFI GGCC 5 cut(s) 878, 1240, 2028, 2129, 2211
BshNI GGYRCC 1 cut(s) 1356
BshVI ATCGAT 1 cut(s) 3716
BsiHKAI GWGCWC 2 cut(s) 74, 3454
BsiHKCI CYCGRG 1 cut(s) 3883
BsiSI CCGG 1 cut(s) 1823
BslFI GGGAC 3 cut(s) 363, 830, 983
BsmAI GTCTC 3 cut(s) 950, 1172, 2571
BsmFI GGGAC 3 cut(s) 363, 830, 983
BsmI GAATGC 2 cut(s) 3023, 3504
BsnI GGCC 5 cut(s) 878, 1240, 2028, 2129, 2211
Bso31I GGTCTC 1 cut(s) 1172
BsoBI CYCGRG 1 cut(s) 3883
Bsp119I TTCGAA 1 cut(s) 166
Bsp1286I GDGCHC 3 cut(s) 74, 2361, 3454
Bsp1720I GCTNAGC 2 cut(s) 1039, 3222
Bsp19I CCATGG 1 cut(s) 1234
BspACI CCGC 3 cut(s) 124, 4015, 4017
BspANI GGCC 5 cut(s) 878, 1240, 2028, 2129, 2211
BspDI ATCGAT 1 cut(s) 3716
BspFNI CGCG 1 cut(s) 4017
BspHI TCATGA 2 cut(s) 205, 1563
BspLI GGNNCC 4 cut(s) 971, 972, 1358, 3132
BspMAI CTGCAG 3 cut(s) 16, 1119, 3196
BspMI ACCTGC 1 cut(s) 2098
BspPI GGATC 4 cut(s) 925, 1347, 2026, 3520
BspT104I TTCGAA 1 cut(s) 166
BspT107I GGYRCC 1 cut(s) 1356
BspTNI GGTCTC 1 cut(s) 1172
BsrDI GCAATG 4 cut(s) 1614, 1732, 1850, 3595
BsrI ACTGG 6 cut(s) 762, 1358, 2470, 2563, 3389, 3734
BssECI CCNNGG 9 cut(s) 252, 778, 934, 1234, 1992, 2171, 2305, 3406, 4015
BssNAI GTATAC 1 cut(s) 1231
BssSI CACGAG 1 cut(s) 1949
BssT1I CCWWGG 4 cut(s) 934, 1234, 1992, 2171
Bst1107I GTATAC 1 cut(s) 1231
Bst2BI CACGAG 1 cut(s) 1949
Bst2UI CCWGG 7 cut(s) 254, 579, 779, 2306, 2481, 2811, 3407
Bst4CI ACNGT 7 cut(s) 367, 745, 1379, 1904, 2674, 2980, 3984
Bst6I CTCTTC 3 cut(s) 182, 1437, 2673
BstBAI YACGTR 2 cut(s) 1132, 1646
BstBI TTCGAA 1 cut(s) 166
BstC8I GCNNGC 8 cut(s) 54, 834, 1488, 1540, 2213, 2217, 2642, 2711
BstDSI CCRYGG 2 cut(s) 1234, 4015
BstENI CCTNNNNNAGG 1 cut(s) 1319
BstFNI CGCG 1 cut(s) 4017
BstHHI GCGC 1 cut(s) 3588
BstMAI GTCTC 3 cut(s) 950, 1172, 2571
BstMWI GCNNNNNNNGC 7 cut(s) 789, 1465, 2356, 2696, 2845, 2927, 3206
BstNI CCWGG 7 cut(s) 254, 579, 779, 2306, 2481, 2811, 3407
BstNSI RCATGY 4 cut(s) 56, 1490, 1542, 3360
BstSCI CCNGG 7 cut(s) 252, 577, 777, 2304, 2479, 2809, 3405
BstSFI CTRYAG 5 cut(s) 12, 1115, 1375, 2444, 3192
BstSNI TACGTA 1 cut(s) 1646
BstUI CGCG 1 cut(s) 4017
BstV1I GCAGC 7 cut(s) 118, 1129, 1813, 2917, 2939, 3415, 3765
BstV2I GAAGAC 2 cut(s) 701, 1762
BstX2I RGATCY 4 cut(s) 930, 1668, 2018, 3991
BstXI CCANNNNNNTGG 3 cut(s) 441, 785, 3165
BstYI RGATCY 4 cut(s) 930, 1668, 2018, 3991
BstZ17I GTATAC 1 cut(s) 1231
Bsu15I ATCGAT 1 cut(s) 3716
BsuI GTATCC 2 cut(s) 3881, 4055
BsuRI GGCC 5 cut(s) 878, 1240, 2028, 2129, 2211
BsuTUI ATCGAT 1 cut(s) 3716
BtgI CCRYGG 2 cut(s) 1234, 4015
BtsI GCAGTG 1 cut(s) 3423
BtsIMutI CAGTG 8 cut(s) 538, 755, 1511, 2495, 2528, 3423, 3727, 4042
BveI ACCTGC 1 cut(s) 2098
Cac8I GCNNGC 8 cut(s) 54, 834, 1488, 1540, 2213, 2217, 2642, 2711
CaiI CAGNNNCTG 2 cut(s) 2021, 2413
CciI TCATGA 2 cut(s) 205, 1563
CfoI GCGC 1 cut(s) 3588
Cfr13I GGNCC 6 cut(s) 87, 572, 970, 1238, 1996, 3957
Cfr42I CCGCGG 1 cut(s) 4018
ClaI ATCGAT 1 cut(s) 3716
CsiI ACCWGGT 1 cut(s) 2809
DraI TTTAAA 1 cut(s) 3352
DrdI GACNNNNNNGTC 1 cut(s) 1424
DriI GACNNNNNGTC 1 cut(s) 1766
DseDI GACNNNNNNGTC 1 cut(s) 1424
EaeI YGGCCR 1 cut(s) 2209
Eam1104I CTCTTC 3 cut(s) 182, 1437, 2673
Eam1105I GACNNNNNGTC 1 cut(s) 1766
EarI CTCTTC 3 cut(s) 182, 1437, 2673
Eco105I TACGTA 1 cut(s) 1646
Eco130I CCWWGG 4 cut(s) 934, 1234, 1992, 2171
Eco24I GRGCYC 1 cut(s) 2361
Eco31I GGTCTC 1 cut(s) 1172
Eco32I GATATC 3 cut(s) 1068, 1743, 2265
Eco47I GGWCC 5 cut(s) 87, 572, 970, 1996, 3957
Eco57I CTGAAG 8 cut(s) 60, 519, 1148, 1236, 1856, 1909, 3484, 3825
Eco88I CYCGRG 1 cut(s) 3883
EcoNI CCTNNNNNAGG 1 cut(s) 1319
EcoO109I RGGNCCY 1 cut(s) 970
EcoRII CCWGG 7 cut(s) 252, 577, 777, 2304, 2479, 2809, 3405
EcoRV GATATC 3 cut(s) 1068, 1743, 2265
EcoT14I CCWWGG 4 cut(s) 934, 1234, 1992, 2171
EcoT22I ATGCAT 2 cut(s) 58, 1864
EcoT38I GRGCYC 1 cut(s) 2361
ErhI CCWWGG 4 cut(s) 934, 1234, 1992, 2171
FalI AAGNNNNNCTT 8 cut(s) 1434, 1466, 1527, 1559, 2745, 2777, 3189, 3221
FaqI GGGAC 3 cut(s) 363, 830, 983
FauI CCCGC 1 cut(s) 4010
FauNDI CATATG 2 cut(s) 2617, 2766
FbaI TGATCA 2 cut(s) 202, 3654
FblI GTMKAC 2 cut(s) 1230, 1791
Fnu4HI GCNGC 8 cut(s) 12, 132, 1118, 1827, 2928, 2931, 3429, 3779
FriOI GRGCYC 1 cut(s) 2361
Fsp4HI GCNGC 8 cut(s) 12, 132, 1118, 1827, 2928, 2931, 3429, 3779
GlaI GCGC 1 cut(s) 3587
GluI GCNGC 8 cut(s) 12, 132, 1118, 1827, 2928, 2931, 3429, 3779
GsaI CCCAGC 2 cut(s) 3083, 3161
GsuI CTGGAG 3 cut(s) 561, 2646, 3406
HaeIII GGCC 5 cut(s) 878, 1240, 2028, 2129, 2211
HapII CCGG 1 cut(s) 1823
HhaI GCGC 1 cut(s) 3588
Hin6I GCGC 1 cut(s) 3586
HinP1I GCGC 1 cut(s) 3586
HincII GTYRAC 1 cut(s) 1663
HindII GTYRAC 1 cut(s) 1663
HindIII AAGCTT 3 cut(s) 457, 1436, 3098
HinfI GANTC 7 cut(s) 559, 1126, 1139, 1840, 2681, 3089, 3236
HpaII CCGG 1 cut(s) 1823
HphI GGTGA 3 cut(s) 1034, 3497, 3664
Hpy166II GTNNAC 6 cut(s) 1231, 1663, 1792, 2313, 2564, 3488
Hpy8I GTNNAC 6 cut(s) 1231, 1663, 1792, 2313, 2564, 3488
Hpy99I CGWCG 1 cut(s) 264
HpyCH4III ACNGT 7 cut(s) 367, 745, 1379, 1904, 2674, 2980, 3984
HpyCH4IV ACGT 4 cut(s) 160, 259, 1131, 1645
HpyF10VI GCNNNNNNNGC 7 cut(s) 789, 1465, 2356, 2696, 2845, 2927, 3206
HpySE526I ACGT 4 cut(s) 160, 259, 1131, 1645
HspAI GCGC 1 cut(s) 3586
KflI GGGWCCC 1 cut(s) 970
Ksp22I TGATCA 2 cut(s) 202, 3654
KspI CCGCGG 1 cut(s) 4018
LmnI GCTCC 7 cut(s) 69, 1091, 2173, 2273, 2513, 3343, 3449
Lsp1109I GCAGC 7 cut(s) 118, 1129, 1813, 2917, 2939, 3415, 3765
LweI GCATC 9 cut(s) 65, 479, 546, 1849, 1963, 1997, 2908, 3440, 4062
MabI ACCWGGT 1 cut(s) 2809
MaeII ACGT 4 cut(s) 160, 259, 1131, 1645
MfeI CAATTG 2 cut(s) 885, 1121
MflI RGATCY 4 cut(s) 930, 1668, 2018, 3991
MhlI GDGCHC 3 cut(s) 74, 2361, 3454
MlsI TGGCCA 1 cut(s) 2211
MluNI TGGCCA 1 cut(s) 2211
MlyI GAGTC 3 cut(s) 553, 1120, 2690
MmeI TCCRAC 2 cut(s) 1408, 2079
Mox20I TGGCCA 1 cut(s) 2211
Mph1103I ATGCAT 2 cut(s) 58, 1864
MroXI GAANNNNTTC 5 cut(s) 162, 414, 1557, 2005, 2760
MscI TGGCCA 1 cut(s) 2211
MseI TTAA 8 cut(s) 624, 1413, 2039, 2226, 2279, 3351, 3419, 3962
MslI CAYNNNNRTG 5 cut(s) 51, 869, 1485, 3244, 3361
Msp20I TGGCCA 1 cut(s) 2211
MspA1I CMGCKG 2 cut(s) 2930, 4017
MspI CCGG 1 cut(s) 1823
MspR9I CCNGG 7 cut(s) 254, 579, 779, 2306, 2481, 2811, 3407
MunI CAATTG 2 cut(s) 885, 1121
Mva1269I GAATGC 2 cut(s) 3023, 3504
MvaI CCWGG 7 cut(s) 254, 579, 779, 2306, 2481, 2811, 3407
MvnI CGCG 1 cut(s) 4017
MwoI GCNNNNNNNGC 7 cut(s) 789, 1465, 2356, 2696, 2845, 2927, 3206
NcoI CCATGG 1 cut(s) 1234
NdeI CATATG 2 cut(s) 2617, 2766
NlaIV GGNNCC 4 cut(s) 971, 972, 1358, 3132
NmuCI GTSAC 6 cut(s) 469, 1244, 2200, 3178, 3732, 3817
NsiI ATGCAT 2 cut(s) 58, 1864
NspI RCATGY 4 cut(s) 56, 1490, 1542, 3360
NspV TTCGAA 1 cut(s) 166
PaeI GCATGC 3 cut(s) 56, 1490, 1542
PagI TCATGA 2 cut(s) 205, 1563
PctI GAATGC 2 cut(s) 3023, 3504
PdmI GAANNNNTTC 5 cut(s) 162, 414, 1557, 2005, 2760
PfeI GAWTC 4 cut(s) 1139, 1840, 3089, 3236
PflMI CCANNNNNTGG 4 cut(s) 584, 1324, 3335, 3719
PkrI GCNGC 8 cut(s) 13, 133, 1119, 1828, 2929, 2932, 3430, 3780
PleI GAGTC 3 cut(s) 553, 1120, 2689
PpsI GAGTC 3 cut(s) 553, 1120, 2689
Ppu21I YACGTR 2 cut(s) 1132, 1646
PpuMI RGGWCCY 1 cut(s) 970
PshBI ATTAAT 1 cut(s) 2226
PsiI TTATAA 1 cut(s) 669
Psp5II RGGWCCY 1 cut(s) 970
Psp6I CCWGG 7 cut(s) 252, 577, 777, 2304, 2479, 2809, 3405
PspFI CCCAGC 2 cut(s) 3079, 3157
PspGI CCWGG 7 cut(s) 252, 577, 777, 2304, 2479, 2809, 3405
PspN4I GGNNCC 4 cut(s) 971, 972, 1358, 3132
PspPI GGNCC 6 cut(s) 87, 572, 970, 1238, 1996, 3957
PspPPI RGGWCCY 1 cut(s) 970
PsrI GAACNNNNNNTAC 2 cut(s) 3267, 3299
PstI CTGCAG 3 cut(s) 16, 1119, 3196
PstNI CAGNNNCTG 2 cut(s) 2021, 2413
PsuI RGATCY 4 cut(s) 930, 1668, 2018, 3991
PvuII CAGCTG 1 cut(s) 2930
RseI CAYNNNNRTG 5 cut(s) 51, 869, 1485, 3244, 3361
SacII CCGCGG 1 cut(s) 4018
SaqAI TTAA 8 cut(s) 624, 1413, 2039, 2226, 2279, 3351, 3419, 3962
SatI GCNGC 8 cut(s) 12, 132, 1118, 1827, 2928, 2931, 3429, 3779
Sau96I GGNCC 6 cut(s) 87, 572, 970, 1238, 1996, 3957
ScaI AGTACT 4 cut(s) 1352, 1682, 2760, 4011
SchI GAGTC 3 cut(s) 553, 1120, 2690
ScrFI CCNGG 7 cut(s) 254, 579, 779, 2306, 2481, 2811, 3407
SduI GDGCHC 3 cut(s) 74, 2361, 3454
SexAI ACCWGGT 1 cut(s) 2809
SfaNI GCATC 9 cut(s) 65, 479, 546, 1849, 1963, 1997, 2908, 3440, 4062
SfcI CTRYAG 5 cut(s) 12, 1115, 1375, 2444, 3192
Sfr303I CCGCGG 1 cut(s) 4018
SfuI TTCGAA 1 cut(s) 166
SgrBI CCGCGG 1 cut(s) 4018
SinI GGWCC 5 cut(s) 87, 572, 970, 1996, 3957
SmiMI CAYNNNNRTG 5 cut(s) 51, 869, 1485, 3244, 3361
SmlI CTYRAG 2 cut(s) 284, 635
SmoI CTYRAG 2 cut(s) 284, 635
SnaBI TACGTA 1 cut(s) 1646
SpeI ACTAGT 2 cut(s) 1621, 4114
SphI GCATGC 3 cut(s) 56, 1490, 1542
SsiI CCGC 3 cut(s) 124, 4015, 4017
SspI AATATT 1 cut(s) 3519
StyD4I CCNGG 7 cut(s) 252, 577, 777, 2304, 2479, 2809, 3405
StyI CCWWGG 4 cut(s) 934, 1234, 1992, 2171
TaaI ACNGT 7 cut(s) 367, 745, 1379, 1904, 2674, 2980, 3984
TaiI ACGT 4 cut(s) 163, 262, 1134, 1648
TaqI TCGA 7 cut(s) 166, 2440, 2828, 3027, 3601, 3698, 3716
TatI WGTACW 7 cut(s) 1350, 1600, 1680, 2758, 2966, 3621, 4009
TfiI GAWTC 4 cut(s) 1139, 1840, 3089, 3236
Tru1I TTAA 8 cut(s) 624, 1413, 2039, 2226, 2279, 3351, 3419, 3962
Tru9I TTAA 8 cut(s) 624, 1413, 2039, 2226, 2279, 3351, 3419, 3962
TscAI CASTG 8 cut(s) 538, 762, 1518, 2502, 2535, 3430, 3734, 4042
TseFI GTSAC 6 cut(s) 469, 1244, 2200, 3178, 3732, 3817
TseI GCWGC 8 cut(s) 11, 131, 1117, 1826, 2927, 2930, 3428, 3778
Tsp45I GTSAC 6 cut(s) 469, 1244, 2200, 3178, 3732, 3817
TspGWI ACGGA 3 cut(s) 2872, 4002, 4016
TspRI CASTG 8 cut(s) 538, 762, 1518, 2502, 2535, 3430, 3734, 4042
Van91I CCANNNNNTGG 4 cut(s) 584, 1324, 3335, 3719
VpaK11BI GGWCC 5 cut(s) 87, 572, 970, 1996, 3957
VspI ATTAAT 1 cut(s) 2226
XagI CCTNNNNNAGG 1 cut(s) 1319
XapI RAATTY 5 cut(s) 385, 516, 987, 2159, 4092
XbaI TCTAGA 3 cut(s) 511, 1547, 1757
XceI RCATGY 4 cut(s) 56, 1490, 1542, 3360
XcmI CCANNNNNNNNNTGG 1 cut(s) 3336
XmiI GTMKAC 2 cut(s) 1230, 1791
XmnI GAANNNNTTC 5 cut(s) 162, 414, 1557, 2005, 2760
ZrmI AGTACT 4 cut(s) 1352, 1682, 2760, 4011
Zsp2I ATGCAT 2 cut(s) 58, 1864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.