Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0000647

Physical Location & Seq

Genomic Coordinates Reverse (-)

23053719 .. 23060435

Gene Structure 6717 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1227200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1527 bp

ATGGCTTGCTCCACCGTTACTCCGCCGTCCACCTCCTTCATCGGCTACAAGAAGGATTTGAATCTCTCTGCTTTCTCCTCTGCGACGCCGCTTTCCAGTCAGAAATGCAAGAGAAAATCCAAGAAAATTTGCTCTGTCATGTCTCCGCAGCAATCGGAGCGCAAGCCCGCCACCACCGGCTCGGTTAAGACCGGGATGACGATAACTGAGAAAATATTTGCTAGAGCTTCTGAGAAAACCCAGCTGAGTCCGGGTGAGAATGTTTGGGTTGATGTCGATGTTTTGATGACCCATGACGTCTGTGGCCCGGGGTCAATTGGAATCTTCAAGAAAGAGTTCGGCCAGAACGCAAAGGTTTGGGACCGTGAAAAGGTTGTAATCATACCGGACCATTATATATTTACAACTGATGAACGTGCAAATCGTAATGTGGATATCTTGAGAGATTTCTGCATGGAGCAAAACATCAAGTATTTCTATGATATCAAGGATCTAAGTAATTTCAAGGCCAACCCTGATTATAAGGGCGTATGCCATGTTGCACTTGCTCAAGAAGGTCATTGCAGGCCGGGGGAGGTCCTGCTGGGTACAGATTCTCATACATGTACTGCTGGAGCTTTTGGACAATTTGCTACTGGAATTGGGAACACTGATGCAGGATTTGTATTGGGCACTGGGAAGCTTTTGCTCAAGGTGCCTCCAACACTGAGATTTGTGATGGATGGTGAAATGCCTGACTATTTGCTTGCCAAAGATTTGATTCTGCAAATTATTGGTGAAATATCTGTTGCTGGTGGAACATATAAAGCTATGGAGTTTGTTGGCAGCACTGTTGAAAGCTTATCTATGGAAGAAAGGATGACATTATGCAACATGGTTGTTGAGGCTGGGGGTAAAAATGGTATTGTTCCTGCTGATAGCACTACATTTAAGTACCTTGAGGATAAGACTTCTGTTCCCTATGAACCTGTTTACAGTGATGCTAAAGCAAGATTTCTTAAGGAGTACAGATTTGATATCTCAAAACTGGAGCCGTTGGTGGCAAAGCCTCATTCTCCAGATAATCGTGCTTTAGCAAGAGAATGCAAAGATGTGAAAATTGACAGAGTATATATCGGTTCTTGTACTGGAGGAAAAACTGAGGATTTTATGGCCGCAGCCAGAGTTTTTCTAGCATCAGGGAAAAAGGTCAAAGTTCCCACATTTCTTGTGCCTGCTACCCAAAAGGTTTGGATGGACGTGTATAGTCTTCCAGTACCAGGGTCTGGTGGCAAGACTTGCTCACAGATATTTGAGGAAGCTGGATGTGACACACCTGCAAGTCCCAGTTGCGGCGCTTGCTTGGGTGGTCCGAAAGACACGTATGCTCGCTTAAATGAACCTCAGGTATGCGTCTCAACAACAAACAGGAACTTCCCAGGCCGAATGGGTCACAAGGAAGGCCAGATATATCTCGCTTCCCCCTATACGGCTGCTGCATCAGCTTTGACTGGTCACGTCACCGACCCAAGAGAGTTTTTGCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010035 GO:0010038 GO:0016143 GO:0016144 GO:0016853 GO:0016866 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044249 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0046686 GO:0050486 GO:0050896 GO:0071704 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659

KEGG Pathways

Metabolic & Signaling

ko00290 ko00660 ko00966 ko01100 ko01110 ko01210 ko01230 map00290 map00660 map00966 map01100 map01110 map01210 map01230

Pfam Domains

Protein Families

Aconitase

Protein Analysis

509

Amino Acids

55.33

Weight (kDa)

8.03

Isoelectric Point (pI)

35.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aconitase	PF00330	71 - 357	1.7e-56	Aconitase family (aconitate hydratase)
Aconitase	PF00330	361 - 498	4e-26	Aconitase family (aconitate hydratase)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000647)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13430
fragaria_vesca	FvH4_4g04720 FvH4_4g04720 FvH4_4g04720 FvH4_4g04720 FvH4_4g04720 FvH4_5g09890
malus_domestica	MD13G1222700.v1.1 MD13G1222900.v1.1 MD16G1227200.v1.1
prunus_persica	Prupe.1G049300_v2.0.a1
pyrus_communis	pycom13g19670 pycom16g19040
rosa_chinensis	RchiOBHm_Chr4g0395521 RchiOBHm_Chr4g0395531 RchiOBHm_Chr6g0260271 RchiOBHm_Chr6g0278291
rosa_laevigata	RLG00000009126 RLG00000009614 RLG00000009616 RLG00000019332 RLG00000035454
rosa_multiflora	Rmu_co8195818.1_g000001 Rmu_co8220062.1_g000001 Rmu_sc0000041.1_g000006 Rmu_sc0000744.1_g000082 Rmu_sc0001142.1_g000007 Rmu_sc0001669.1_g000055 Rmu_sc0003400.1_g000015 Rmu_sc0005229.1_g000033 Rmu_sc0007340.1_g000006 Rmu_sc0008351.1_g000002 Rmu_sc0008351.1_g000016 Rmu_sc0039027.1_g000001
rosa_roxburghii	Rroxscaffold_4G00324270 Rroxscaffold_5G00340460 Rroxscaffold_5G00340480
rosa_rugosa	Rorug01G0023800 Rorug01G0050600 Rorug02G0280700 Rorug03G0355400 Rorug07G0181700
rosa_samantha	Rh1CG053700 Rh1DG057900 Rh3CG159300 Rh3DG159800 Rh3DG297100 Rh4AG060800 Rh4AG061300 Rh4AG061500 Rh4BG059800 Rh4BG060100 Rh4CG065500 Rh4CG065800 Rh4DG055700 Rh4DG056000 Rh5BG283600 Rh5BG284500 Rh5BG323300 Rh5BG330600 Rh5BG516600 Rh5CG076000 Rh5CG289900 Rh5DG060900 Rh5DG293300 Rh5DG293900 Rh5DG334300 Rh5DG342000 Rh6AG129800 Rh6DG223600
rosa_wichuraiana	Rw0G000910 Rw2G008950 Rw2G012250 Rw3G021630 Rw4G005010 Rw4G005020 Rw4G005030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	522
AarI	CACCTGC	1 cut(s)	1324
AatII	GACGTC	1 cut(s)	300
Acc36I	ACCTGC	1 cut(s)	1324
AccB1I	GGYRCC	1 cut(s)	694
AccB7I	CCANNNNNTGG	1 cut(s)	1265
AciI	CCGC	6 cut(s)	23, 89, 146, 168, 1155, 1332
AclWI	GGATC	1 cut(s)	498
AcoI	YGGCCR	2 cut(s)	340, 1152
AcsI	RAATTY	1 cut(s)	126
AcyI	GRCGYC	2 cut(s)	86, 297
AfaI	GTAC	6 cut(s)	589, 607, 935, 1007, 1126, 1257
AfiI	CCNNNNNNNGG	5 cut(s)	370, 1259, 1265, 1331, 1468
AflII	CTTAAG	1 cut(s)	998
AflIII	ACRYGT	3 cut(s)	602, 1239, 1359
AgsI	TTSAA	4 cut(s)	61, 328, 505, 836
AjiI	CACGTC	2 cut(s)	1240, 1498
AjnI	CCWGG	2 cut(s)	1258, 1417
AluBI	AGCT	8 cut(s)	227, 244, 617, 682, 809, 840, 1301, 1484
AluI	AGCT	8 cut(s)	227, 244, 617, 682, 809, 840, 1301, 1484
Alw26I	GTCTC	2 cut(s)	147, 1399
AlwI	GGATC	1 cut(s)	498
AlwNI	CAGNNNCTG	1 cut(s)	1265
Ama87I	CYCGRG	1 cut(s)	307
AoxI	GGCC	7 cut(s)	304, 340, 507, 566, 1152, 1420, 1441
ApeKI	GCWGC	5 cut(s)	148, 825, 1157, 1472, 1475
ApoI	RAATTY	1 cut(s)	126
Asp700I	GAANNNNTTC	1 cut(s)	335
AspLEI	GCGC	2 cut(s)	162, 1337
AspS9I	GGNCC	5 cut(s)	305, 361, 388, 577, 1349
AsuC2I	CCSGG	5 cut(s)	193, 252, 308, 309, 570
AsuHPI	GGTGA	4 cut(s)	266, 737, 788, 1492
AvaI	CYCGRG	1 cut(s)	307
AvaII	GGWCC	4 cut(s)	361, 388, 577, 1349
AxyI	CCTNAGG	1 cut(s)	1383
BaeGI	GKGCMC	1 cut(s)	674
BanI	GGYRCC	1 cut(s)	694
BbsI	GAAGAC	1 cut(s)	1241
BbvI	GCAGC	5 cut(s)	160, 837, 1169, 1459, 1462
BccI	CCATC	3 cut(s)	712, 716, 1228
BceAI	ACGGC	3 cut(s)	10, 1018, 1485
BciT130I	CCWGG	2 cut(s)	1260, 1419
BcnI	CCSGG	5 cut(s)	193, 252, 308, 309, 570
BcoDI	GTCTC	2 cut(s)	147, 1399
BfaI	CTAG	2 cut(s)	222, 1172
BfoI	RGCGCY	1 cut(s)	1338
BfrI	CTTAAG	1 cut(s)	998
BfuAI	ACCTGC	1 cut(s)	1324
BisI	GCNGC	8 cut(s)	89, 149, 826, 1155, 1158, 1333, 1473, 1476
BlsI	GCNGC	8 cut(s)	90, 150, 827, 1156, 1159, 1334, 1474, 1477
Bme1390I	CCNGG	7 cut(s)	193, 252, 308, 309, 570, 1260, 1419
Bme18I	GGWCC	4 cut(s)	361, 388, 577, 1349
BmeT110I	CYCGRG	1 cut(s)	307
BmgBI	CACGTC	2 cut(s)	1240, 1498
BmgT120I	GGNCC	5 cut(s)	305, 361, 388, 577, 1349
BmiI	GGNNCC	3 cut(s)	362, 696, 1032
BmrFI	CCNGG	7 cut(s)	193, 252, 308, 309, 570, 1260, 1419
BmrI	ACTGGG	2 cut(s)	684, 1320
BmsI	GCATC	4 cut(s)	643, 970, 1184, 1487
BmuI	ACTGGG	2 cut(s)	684, 1320
BpiI	GAAGAC	1 cut(s)	1241
BpmI	CTGGAG	4 cut(s)	633, 1041, 1049, 1149
BpuEI	CTTGAG	4 cut(s)	460, 534, 674, 959
BpuMI	CCSGG	5 cut(s)	193, 252, 308, 309, 570
BsaAI	YACGTR	1 cut(s)	1362
BsaBI	GATNNNNATC	1 cut(s)	60
BsaHI	GRCGYC	2 cut(s)	86, 297
BsaJI	CCNNGG	5 cut(s)	307, 308, 569, 1259, 1417
BsaWI	WCCGGW	1 cut(s)	385
BsaXI	ACNNNNNCTCC	1 cut(s)	34
Bsc4I	CCNNNNNNNGG	5 cut(s)	370, 1259, 1265, 1331, 1468
Bse118I	RCCGGY	1 cut(s)	176
Bse1I	ACTGG	8 cut(s)	96, 640, 679, 1032, 1132, 1253, 1326, 1495
Bse21I	CCTNAGG	1 cut(s)	1383
Bse3DI	GCAATG	1 cut(s)	559
Bse8I	GATNNNNATC	1 cut(s)	60
BseBI	CCWGG	2 cut(s)	1260, 1419
BseDI	CCNNGG	5 cut(s)	307, 308, 569, 1259, 1417
BseGI	GGATG	5 cut(s)	201, 727, 864, 1239, 1310
BseJI	GATNNNNATC	1 cut(s)	60
BseLI	CCNNNNNNNGG	5 cut(s)	370, 1259, 1265, 1331, 1468
BseMI	GCAATG	1 cut(s)	559
BseMII	CTCAG	6 cut(s)	198, 222, 236, 698, 1131, 1397
BseNI	ACTGG	8 cut(s)	96, 640, 679, 1032, 1132, 1253, 1326, 1495
BseRI	GAGGAG	1 cut(s)	67
BseSI	GKGCMC	1 cut(s)	674
BseXI	GCAGC	5 cut(s)	160, 837, 1169, 1459, 1462
BseYI	CCCAGC	3 cut(s)	240, 583, 887
BshFI	GGCC	7 cut(s)	306, 342, 509, 568, 1154, 1422, 1443
BshNI	GGYRCC	1 cut(s)	694
BsiHKCI	CYCGRG	1 cut(s)	307
BsiSI	CCGG	6 cut(s)	177, 192, 251, 308, 386, 569
BslFI	GGGAC	2 cut(s)	374, 1308
BslI	CCNNNNNNNGG	5 cut(s)	370, 1259, 1265, 1331, 1468
BsmAI	GTCTC	2 cut(s)	147, 1399
BsmBI	CGTCTC	1 cut(s)	1399
BsmFI	GGGAC	2 cut(s)	374, 1308
BsmI	GAATGC	1 cut(s)	1088
BsnI	GGCC	7 cut(s)	306, 342, 509, 568, 1154, 1422, 1443
BsoBI	CYCGRG	1 cut(s)	307
Bsp1286I	GDGCHC	1 cut(s)	674
Bsp143I	GATC	1 cut(s)	490
BspACI	CCGC	6 cut(s)	23, 89, 146, 168, 1155, 1332
BspANI	GGCC	7 cut(s)	306, 342, 509, 568, 1154, 1422, 1443
BspCNI	CTCAG	6 cut(s)	199, 223, 237, 699, 1132, 1396
BspLI	GGNNCC	3 cut(s)	362, 696, 1032
BspMI	ACCTGC	1 cut(s)	1324
BspPI	GGATC	1 cut(s)	498
BspT107I	GGYRCC	1 cut(s)	694
BspTI	CTTAAG	1 cut(s)	998
BsrDI	GCAATG	1 cut(s)	559
BsrFI	RCCGGY	1 cut(s)	176
BsrI	ACTGG	8 cut(s)	96, 640, 679, 1032, 1132, 1253, 1326, 1495
BssAI	RCCGGY	1 cut(s)	176
BssECI	CCNNGG	5 cut(s)	307, 308, 569, 1259, 1417
BssMI	GATC	1 cut(s)	490
BssNI	GRCGYC	2 cut(s)	86, 297
Bst2UI	CCWGG	2 cut(s)	1260, 1419
Bst4CI	ACNGT	4 cut(s)	16, 365, 832, 977
BstACI	GRCGYC	2 cut(s)	86, 297
BstAFI	CTTAAG	1 cut(s)	998
BstAPI	GCANNNNNTGC	1 cut(s)	1278
BstBAI	YACGTR	1 cut(s)	1362
BstC8I	GCNNGC	8 cut(s)	7, 164, 168, 566, 747, 1215, 1339, 1369
BstDEI	CTNAG	7 cut(s)	207, 231, 245, 494, 707, 1140, 1383
BstF5I	GGATG	5 cut(s)	201, 727, 864, 1239, 1310
BstH2I	RGCGCY	1 cut(s)	1338
BstHHI	GCGC	2 cut(s)	162, 1337
BstKTI	GATC	1 cut(s)	493
BstMAI	GTCTC	2 cut(s)	147, 1399
BstMBI	GATC	1 cut(s)	490
BstMWI	GCNNNNNNNGC	5 cut(s)	157, 694, 1278, 1338, 1481
BstNI	CCWGG	2 cut(s)	1260, 1419
BstNSI	RCATGY	1 cut(s)	606
BstSCI	CCNGG	7 cut(s)	191, 250, 306, 307, 568, 1258, 1417
BstSLI	GKGCMC	1 cut(s)	674
BstV1I	GCAGC	5 cut(s)	160, 837, 1169, 1459, 1462
BstV2I	GAAGAC	1 cut(s)	1241
BstX2I	RGATCY	1 cut(s)	490
BstYI	RGATCY	1 cut(s)	490
Bsu36I	CCTNAGG	1 cut(s)	1383
BsuRI	GGCC	7 cut(s)	306, 342, 509, 568, 1154, 1422, 1443
BtrI	CACGTC	2 cut(s)	1240, 1498
BtsCI	GGATG	5 cut(s)	201, 727, 864, 1239, 1310
BtsIMutI	CAGTG	5 cut(s)	648, 672, 704, 828, 982
BveI	ACCTGC	1 cut(s)	1324
Cac8I	GCNNGC	8 cut(s)	7, 164, 168, 566, 747, 1215, 1339, 1369
CaiI	CAGNNNCTG	1 cut(s)	1265
CfoI	GCGC	2 cut(s)	162, 1337
Cfr10I	RCCGGY	1 cut(s)	176
Cfr13I	GGNCC	5 cut(s)	305, 361, 388, 577, 1349
Cfr9I	CCCGGG	1 cut(s)	307
CseI	GACGC	2 cut(s)	94, 1381
Csp6I	GTAC	6 cut(s)	588, 606, 934, 1006, 1125, 1256
CspCI	CAANNNNNGTGG	2 cut(s)	1189, 1224
CviAII	CATG	6 cut(s)	139, 293, 454, 536, 603, 874
CviQI	GTAC	6 cut(s)	588, 606, 934, 1006, 1125, 1256
DdeI	CTNAG	7 cut(s)	207, 231, 245, 494, 707, 1140, 1383
DpnI	GATC	1 cut(s)	492
DpnII	GATC	1 cut(s)	490
EaeI	YGGCCR	2 cut(s)	340, 1152
EciI	GGCGGA	1 cut(s)	12
Eco32I	GATATC	3 cut(s)	436, 484, 1018
Eco47I	GGWCC	4 cut(s)	361, 388, 577, 1349
Eco81I	CCTNAGG	1 cut(s)	1383
Eco88I	CYCGRG	1 cut(s)	307
EcoO109I	RGGNCCY	1 cut(s)	577
EcoRII	CCWGG	2 cut(s)	1258, 1417
EcoRV	GATATC	3 cut(s)	436, 484, 1018
Esp3I	CGTCTC	1 cut(s)	1399
FaeI	CATG	6 cut(s)	142, 296, 457, 539, 606, 877
FaqI	GGGAC	2 cut(s)	374, 1308
FatI	CATG	6 cut(s)	138, 292, 453, 535, 602, 873
FauI	CCCGC	1 cut(s)	175
Fnu4HI	GCNGC	8 cut(s)	89, 149, 826, 1155, 1158, 1333, 1473, 1476
FokI	GGATG	5 cut(s)	208, 734, 871, 1246, 1317
Fsp4HI	GCNGC	8 cut(s)	89, 149, 826, 1155, 1158, 1333, 1473, 1476
FspBI	CTAG	2 cut(s)	222, 1172
GlaI	GCGC	2 cut(s)	161, 1336
GluI	GCNGC	8 cut(s)	89, 149, 826, 1155, 1158, 1333, 1473, 1476
GsaI	CCCAGC	3 cut(s)	244, 587, 891
GsuI	CTGGAG	4 cut(s)	633, 1041, 1049, 1149
HaeII	RGCGCY	1 cut(s)	1338
HaeIII	GGCC	7 cut(s)	306, 342, 509, 568, 1154, 1422, 1443
HapII	CCGG	6 cut(s)	177, 192, 251, 308, 386, 569
HgaI	GACGC	2 cut(s)	94, 1381
HhaI	GCGC	2 cut(s)	162, 1337
Hin1I	GRCGYC	2 cut(s)	86, 297
Hin1II	CATG	6 cut(s)	142, 296, 457, 539, 606, 877
Hin6I	GCGC	2 cut(s)	160, 1335
HinP1I	GCGC	2 cut(s)	160, 1335
HindIII	AAGCTT	2 cut(s)	680, 838
HinfI	GANTC	5 cut(s)	61, 247, 321, 593, 760
HpaII	CCGG	6 cut(s)	177, 192, 251, 308, 386, 569
HphI	GGTGA	4 cut(s)	266, 737, 788, 1492
Hpy166II	GTNNAC	2 cut(s)	30, 973
Hpy188I	TCNGA	4 cut(s)	102, 157, 232, 1353
Hpy188III	TCNNGA	4 cut(s)	328, 439, 551, 1058
Hpy8I	GTNNAC	2 cut(s)	30, 973
Hpy99I	CGWCG	1 cut(s)	88
HpyAV	CCTTC	4 cut(s)	46, 46, 548, 1433
HpyCH4III	ACNGT	4 cut(s)	16, 365, 832, 977
HpyCH4IV	ACGT	5 cut(s)	297, 415, 1239, 1361, 1497
HpyF10VI	GCNNNNNNNGC	5 cut(s)	157, 694, 1278, 1338, 1481
HpyF3I	CTNAG	7 cut(s)	207, 231, 245, 494, 707, 1140, 1383
HpySE526I	ACGT	5 cut(s)	297, 415, 1239, 1361, 1497
Hsp92I	GRCGYC	2 cut(s)	86, 297
Hsp92II	CATG	6 cut(s)	142, 296, 457, 539, 606, 877
HspAI	GCGC	2 cut(s)	160, 1335
Kzo9I	GATC	1 cut(s)	490
LmnI	GCTCC	5 cut(s)	14, 157, 457, 614, 1030
Lsp1109I	GCAGC	5 cut(s)	160, 837, 1169, 1459, 1462
LweI	GCATC	4 cut(s)	643, 970, 1184, 1487
MaeI	CTAG	2 cut(s)	222, 1172
MaeII	ACGT	5 cut(s)	297, 415, 1239, 1361, 1497
MaeIII	GTNAC	5 cut(s)	16, 1307, 1430, 1493, 1498
MalI	GATC	1 cut(s)	492
MboI	GATC	1 cut(s)	490
MboII	GAAGA	3 cut(s)	316, 863, 1241
MfeI	CAATTG	1 cut(s)	315
MflI	RGATCY	1 cut(s)	490
MhlI	GDGCHC	1 cut(s)	674
MluCI	AATT	7 cut(s)	126, 315, 499, 626, 639, 768, 1098
MlyI	GAGTC	1 cut(s)	256
MmeI	TCCRAC	1 cut(s)	725
MroXI	GAANNNNTTC	1 cut(s)	335
MseI	TTAA	4 cut(s)	186, 930, 999, 1373
MspA1I	CMGCKG	1 cut(s)	244
MspCI	CTTAAG	1 cut(s)	998
MspI	CCGG	6 cut(s)	177, 192, 251, 308, 386, 569
MspR9I	CCNGG	7 cut(s)	193, 252, 308, 309, 570, 1260, 1419
MunI	CAATTG	1 cut(s)	315
Mva1269I	GAATGC	1 cut(s)	1088
MvaI	CCWGG	2 cut(s)	1260, 1419
MwoI	GCNNNNNNNGC	5 cut(s)	157, 694, 1278, 1338, 1481
NciI	CCSGG	5 cut(s)	193, 252, 308, 309, 570
NdeII	GATC	1 cut(s)	490
NlaIII	CATG	6 cut(s)	142, 296, 457, 539, 606, 877
NlaIV	GGNNCC	3 cut(s)	362, 696, 1032
NmuCI	GTSAC	4 cut(s)	1307, 1430, 1493, 1498
NspI	RCATGY	1 cut(s)	606
PaqCI	CACCTGC	1 cut(s)	1324
PciI	ACATGT	1 cut(s)	602
PcsI	WCGNNNNNNNCGW	1 cut(s)	421
PctI	GAATGC	1 cut(s)	1088
PdmI	GAANNNNTTC	1 cut(s)	335
PfeI	GAWTC	4 cut(s)	61, 321, 593, 760
PflMI	CCANNNNNTGG	1 cut(s)	1265
PkrI	GCNGC	8 cut(s)	90, 150, 827, 1156, 1159, 1334, 1474, 1477
PleI	GAGTC	1 cut(s)	255
PpsI	GAGTC	1 cut(s)	255
Ppu21I	YACGTR	1 cut(s)	1362
PpuMI	RGGWCCY	1 cut(s)	577
PscI	ACATGT	1 cut(s)	602
PsiI	TTATAA	1 cut(s)	522
Psp5II	RGGWCCY	1 cut(s)	577
Psp6I	CCWGG	2 cut(s)	1258, 1417
PspFI	CCCAGC	3 cut(s)	240, 583, 887
PspGI	CCWGG	2 cut(s)	1258, 1417
PspN4I	GGNNCC	3 cut(s)	362, 696, 1032
PspPI	GGNCC	5 cut(s)	305, 361, 388, 577, 1349
PspPPI	RGGWCCY	1 cut(s)	577
PstNI	CAGNNNCTG	1 cut(s)	1265
PsuI	RGATCY	1 cut(s)	490
PvuII	CAGCTG	1 cut(s)	244
RsaI	GTAC	6 cut(s)	589, 607, 935, 1007, 1126, 1257
RsaNI	GTAC	6 cut(s)	588, 606, 934, 1006, 1125, 1256
SaqAI	TTAA	4 cut(s)	186, 930, 999, 1373
SatI	GCNGC	8 cut(s)	89, 149, 826, 1155, 1158, 1333, 1473, 1476
Sau3AI	GATC	1 cut(s)	490
Sau96I	GGNCC	5 cut(s)	305, 361, 388, 577, 1349
SchI	GAGTC	1 cut(s)	256
ScrFI	CCNGG	7 cut(s)	193, 252, 308, 309, 570, 1260, 1419
SduI	GDGCHC	1 cut(s)	674
SfaNI	GCATC	4 cut(s)	643, 970, 1184, 1487
SinI	GGWCC	4 cut(s)	361, 388, 577, 1349
SmaI	CCCGGG	1 cut(s)	309
SmlI	CTYRAG	5 cut(s)	439, 549, 689, 938, 998
SmoI	CTYRAG	5 cut(s)	439, 549, 689, 938, 998
Sse9I	AATT	7 cut(s)	126, 315, 499, 626, 639, 768, 1098
SsiI	CCGC	6 cut(s)	23, 89, 146, 168, 1155, 1332
SspI	AATATT	1 cut(s)	216
SspMI	CTAG	2 cut(s)	222, 1172
StyD4I	CCNGG	7 cut(s)	191, 250, 306, 307, 568, 1258, 1417
TaaI	ACNGT	4 cut(s)	16, 365, 832, 977
TaiI	ACGT	5 cut(s)	300, 418, 1242, 1364, 1500
TaqI	TCGA	1 cut(s)	276
TasI	AATT	7 cut(s)	126, 315, 499, 626, 639, 768, 1098
TatI	WGTACW	3 cut(s)	605, 1005, 1124
TauI	GCSGC	3 cut(s)	91, 1157, 1335
TfiI	GAWTC	4 cut(s)	61, 321, 593, 760
Tru1I	TTAA	4 cut(s)	186, 930, 999, 1373
Tru9I	TTAA	4 cut(s)	186, 930, 999, 1373
TscAI	CASTG	5 cut(s)	655, 679, 711, 835, 982
TseFI	GTSAC	4 cut(s)	1307, 1430, 1493, 1498
TseI	GCWGC	5 cut(s)	148, 825, 1157, 1472, 1475
Tsp45I	GTSAC	4 cut(s)	1307, 1430, 1493, 1498
TspDTI	ATGAA	4 cut(s)	28, 426, 978, 1392
TspMI	CCCGGG	1 cut(s)	307
TspRI	CASTG	5 cut(s)	655, 679, 711, 835, 982
Van91I	CCANNNNNTGG	1 cut(s)	1265
Vha464I	CTTAAG	1 cut(s)	998
VpaK11BI	GGWCC	4 cut(s)	361, 388, 577, 1349
XapI	RAATTY	1 cut(s)	126
XceI	RCATGY	1 cut(s)	606
XcmI	CCANNNNNNNNNTGG	1 cut(s)	299
XmaI	CCCGGG	1 cut(s)	307
XmnI	GAANNNNTTC	1 cut(s)	335
XspI	CTAG	2 cut(s)	222, 1172
ZraI	GACGTC	1 cut(s)	298

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.