Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0000647

Physical Location & Seq

Genomic Coordinates Forward (+)

10102143 .. 10107589

Gene Structure 5447 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G005020.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1473 bp

ATGGTCTGCTCTCTCGTGATTCTCGGTTCCCCCACGGGAACCACCAGAAACATAAAAGAACTACAAAAAACAAAACAACAACTAAAAAGAGGAGCCTCTAAAACAACTATAACTAAGCTGTCAAATAAAGCAGCAGCAAAGGATTTGGGTCTCTCTGCTTTCTCTTCCACGTCGCCGGCTTCATTTCCGAGATGCAAGAGATCGATCTCCAAGAAAATCTGCTCCGTCATGGCGCCACAGCAATCGGAGCGGAAGCCAGCCACTACCGGCTCGAAGATATTGGCTAGGGCTTCTGAGAAACCCCAATTGAGCCCAGGTGACAATGTTTGGGTTAACATTGATGTTTTGATGACCCATGACGTGTGTGGCCCTGGTTCATTTGGAATCTTCAAGAAAGAGGATTTCTGCACGGAGCAGAACATCAAGTACTTCTATGATATCAAGGATCTTGGTAACTTTAAGGCTAACCCAGATTACAAGGGTGTATGCCATGTTGCTCTTGCTCAAGAAGGTCATTGCAGGCCTGGAGAGACATTTTTGTTGGCTTCAGGTGCTGCTGGTACAGATTCTCACACCTGCACCGCTGGAGCTTTTGGACAATTTGCTACTGGAATCGGGAATACTGATGCAGGTTTCGTATTGGGCACTGGGAAGCTTTTGCTCAAGGTGCCTCCAACTCTGAGATTTGTGCTGGATGGTGAAATGCCTGACTATTTGCTTGCCAAAGACTTGATTTTACAAATTATTGGTGAAATCTCTGTTGCTGGTGCAACATATAAAGCTATGGAGTTTGTTGGCAGCACTGTTGAAAGCTTAAGTATGGAAGAGAGGATGACATTATGCAACATGGTTGTTGAAGCTGGGGGTAAAAACGGTATGTCCCTGCTGATAGCACTACATACAAGTACCTTGAGGTTAGATAAAACGTCTGTACCCTATGAACCTGTGTACAGTGATGATACAGCAAGATTTCTTACTGAGTACAGATTTGATATCTCAAAACTGGAGCCATTGGTGGCAAAGCCTCATTCTCCCGATAACCGTGCTTTAGCAAGAGAATGCAAAGATGTGAAAATTGACAGAGTCTATATTGGATCTTGTACTGGTGGGAAAACAGAGGATTTTATGGCTGCTGCCAAAGTTTTTCTAGCTTCGGTCAAAGTACCCACTTTTCTTGTACCTGCTACACAAAAGTCTTCCAGTGCCAGGATCAGGTGGCAAGACTTGCTCACAGATATTGAGGAAGCTGGTTGTGACACACCTGCAAGTCCCAGTTGTGGTGCTTGCTTGGGTGGTCCTAAGGACACGTATGCTCGCTTGAATGAACCACAGGTATGTGTGTCTACTACGAACAGGAACTTCCCAGGCAGAATGGGGCACAAGGAAGGACAGATATATCTTGCTTCACCATATACAGCTGCAGCATCTGCTTTGACCGGTTATGTTACCGACCCAAGAGAGTTTTTGCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010035 GO:0010038 GO:0016143 GO:0016144 GO:0016853 GO:0016866 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044249 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0046686 GO:0050486 GO:0050896 GO:0071704 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659

KEGG Pathways

Metabolic & Signaling

ko00290 ko00660 ko00966 ko01100 ko01110 ko01210 ko01230 map00290 map00660 map00966 map01100 map01110 map01210 map01230

Pfam Domains

Protein Families

Aconitase

Protein Analysis

490

Amino Acids

52.8

Weight (kDa)

8.59

Isoelectric Point (pI)

43.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aconitase	PF00330	152 - 348	1.8e-42	Aconitase family (aconitate hydratase)
Aconitase	PF00330	353 - 480	1.7e-25	Aconitase family (aconitate hydratase)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000647)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13430
fragaria_vesca	FvH4_4g04720 FvH4_4g04720 FvH4_4g04720 FvH4_4g04720 FvH4_4g04720 FvH4_5g09890
malus_domestica	MD13G1222700.v1.1 MD13G1222900.v1.1 MD16G1227200.v1.1
prunus_persica	Prupe.1G049300_v2.0.a1
pyrus_communis	pycom13g19670 pycom16g19040
rosa_chinensis	RchiOBHm_Chr4g0395521 RchiOBHm_Chr4g0395531 RchiOBHm_Chr6g0260271 RchiOBHm_Chr6g0278291
rosa_laevigata	RLG00000009126 RLG00000009614 RLG00000009616 RLG00000019332 RLG00000035454
rosa_multiflora	Rmu_co8195818.1_g000001 Rmu_co8220062.1_g000001 Rmu_sc0000041.1_g000006 Rmu_sc0000744.1_g000082 Rmu_sc0001142.1_g000007 Rmu_sc0001669.1_g000055 Rmu_sc0003400.1_g000015 Rmu_sc0005229.1_g000033 Rmu_sc0007340.1_g000006 Rmu_sc0008351.1_g000002 Rmu_sc0008351.1_g000016 Rmu_sc0039027.1_g000001
rosa_roxburghii	Rroxscaffold_4G00324270 Rroxscaffold_5G00340460 Rroxscaffold_5G00340480
rosa_rugosa	Rorug01G0023800 Rorug01G0050600 Rorug02G0280700 Rorug03G0355400 Rorug07G0181700
rosa_samantha	Rh1CG053700 Rh1DG057900 Rh3CG159300 Rh3DG159800 Rh3DG297100 Rh4AG060800 Rh4AG061300 Rh4AG061500 Rh4BG059800 Rh4BG060100 Rh4CG065500 Rh4CG065800 Rh4DG055700 Rh4DG056000 Rh5BG283600 Rh5BG284500 Rh5BG323300 Rh5BG330600 Rh5BG516600 Rh5CG076000 Rh5CG289900 Rh5DG060900 Rh5DG293300 Rh5DG293900 Rh5DG334300 Rh5DG342000 Rh6AG129800 Rh6DG223600
rosa_wichuraiana	Rw0G000910 Rw2G008950 Rw2G012250 Rw3G021630 Rw4G005010 Rw4G005020 Rw4G005030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	584, 1270
Acc36I	ACCTGC	4 cut(s)	584, 620, 1189, 1270
AccB1I	GGYRCC	2 cut(s)	232, 667
AccBSI	CCGCTC	1 cut(s)	250
AccI	GTMKAC	1 cut(s)	1343
AciI	CCGC	2 cut(s)	250, 582
AclWI	GGATC	3 cut(s)	453, 1102, 1217
AcuI	CTGAAG	1 cut(s)	531
AcyI	GRCGYC	1 cut(s)	233
AfaI	GTAC	9 cut(s)	428, 562, 907, 933, 950, 983, 1102, 1164, 1179
AfiI	CCNNNNNNNGG	3 cut(s)	1206, 1212, 1277
AflII	CTTAAG	1 cut(s)	814
AflIII	ACRYGT	2 cut(s)	360, 1305
AgeI	ACCGGT	1 cut(s)	1436
AgsI	TTSAA	4 cut(s)	391, 809, 857, 1321
AjiI	CACGTC	2 cut(s)	171, 361
AjnI	CCWGG	5 cut(s)	313, 370, 523, 1205, 1363
AluBI	AGCT	9 cut(s)	118, 590, 655, 782, 813, 860, 1151, 1247, 1418
AluI	AGCT	9 cut(s)	118, 590, 655, 782, 813, 860, 1151, 1247, 1418
Alw26I	GTCTC	2 cut(s)	155, 524
AlwI	GGATC	3 cut(s)	453, 1102, 1217
AlwNI	CAGNNNCTG	2 cut(s)	554, 1427
AoxI	GGCC	2 cut(s)	367, 521
ApeKI	GCWGC	8 cut(s)	131, 134, 554, 798, 1130, 1133, 1418, 1421
AsiGI	ACCGGT	1 cut(s)	1436
AspLEI	GCGC	1 cut(s)	235
AspS9I	GGNCC	2 cut(s)	368, 1295
AsuHPI	GGTGA	4 cut(s)	329, 710, 761, 1398
AvaII	GGWCC	1 cut(s)	1295
AxyI	CCTNAGG	1 cut(s)	1299
BaeGI	GKGCMC	2 cut(s)	647, 1380
BaeI	ACNNNNGTAYC	2 cut(s)	915, 948
BanI	GGYRCC	2 cut(s)	232, 667
BanII	GRGCYC	1 cut(s)	314
BauI	CACGAG	1 cut(s)	14
BbsI	GAAGAC	1 cut(s)	1188
BbvI	GCAGC	8 cut(s)	143, 146, 541, 810, 1117, 1120, 1405, 1433
BccI	CCATC	1 cut(s)	689
BcgI	CGANNNNNNTGC	2 cut(s)	1025, 1059
BciT130I	CCWGG	5 cut(s)	315, 372, 525, 1207, 1365
BcoDI	GTCTC	2 cut(s)	155, 524
BfaI	CTAG	2 cut(s)	285, 1148
BfmI	CTRYAG	1 cut(s)	1419
BfoI	RGCGCY	1 cut(s)	236
BfrI	CTTAAG	1 cut(s)	814
BfuAI	ACCTGC	4 cut(s)	584, 620, 1189, 1270
BisI	GCNGC	8 cut(s)	132, 135, 555, 799, 1131, 1134, 1419, 1422
BlsI	GCNGC	8 cut(s)	133, 136, 556, 800, 1132, 1135, 1420, 1423
BmcAI	AGTACT	1 cut(s)	428
Bme1390I	CCNGG	5 cut(s)	315, 372, 525, 1207, 1365
Bme18I	GGWCC	1 cut(s)	1295
BmgBI	CACGTC	2 cut(s)	171, 361
BmgT120I	GGNCC	2 cut(s)	368, 1295
BmiI	GGNNCC	6 cut(s)	28, 40, 94, 234, 669, 1008
BmrFI	CCNGG	5 cut(s)	315, 372, 525, 1207, 1365
BmrI	ACTGGG	2 cut(s)	657, 1266
BmsI	GCATC	3 cut(s)	182, 616, 1433
BmuI	ACTGGG	2 cut(s)	657, 1266
BpiI	GAAGAC	1 cut(s)	1188
BpmI	CTGGAG	3 cut(s)	546, 606, 1025
BpuEI	CTTGAG	3 cut(s)	489, 647, 931
Bsa29I	ATCGAT	1 cut(s)	203
BsaAI	YACGTR	1 cut(s)	1308
BsaHI	GRCGYC	1 cut(s)	233
BsaI	GGTCTC	1 cut(s)	155
BsaJI	CCNNGG	4 cut(s)	33, 313, 370, 1363
BsaWI	WCCGGW	1 cut(s)	1436
Bsc4I	CCNNNNNNNGG	3 cut(s)	1206, 1212, 1277
Bse118I	RCCGGY	3 cut(s)	175, 266, 1436
Bse1I	ACTGG	6 cut(s)	613, 652, 1008, 1108, 1200, 1272
Bse21I	CCTNAGG	1 cut(s)	1299
Bse3DI	GCAATG	1 cut(s)	514
BseBI	CCWGG	5 cut(s)	315, 372, 525, 1207, 1365
BseCI	ATCGAT	1 cut(s)	203
BseDI	CCNNGG	4 cut(s)	33, 313, 370, 1363
BseGI	GGATG	2 cut(s)	700, 837
BseLI	CCNNNNNNNGG	3 cut(s)	1206, 1212, 1277
BseMI	GCAATG	1 cut(s)	514
BseMII	CTCAG	3 cut(s)	285, 671, 969
BseNI	ACTGG	6 cut(s)	613, 652, 1008, 1108, 1200, 1272
BseRI	GAGGAG	1 cut(s)	105
BseSI	GKGCMC	2 cut(s)	647, 1380
BseXI	GCAGC	8 cut(s)	143, 146, 541, 810, 1117, 1120, 1405, 1433
BseYI	CCCAGC	1 cut(s)	860
BsgI	GTGCAG	2 cut(s)	391, 562
BshFI	GGCC	2 cut(s)	369, 523
BshNI	GGYRCC	2 cut(s)	232, 667
BshTI	ACCGGT	1 cut(s)	1436
BshVI	ATCGAT	1 cut(s)	203
BsiSI	CCGG	3 cut(s)	176, 267, 1437
BslFI	GGGAC	2 cut(s)	865, 1254
BslI	CCNNNNNNNGG	3 cut(s)	1206, 1212, 1277
BsmAI	GTCTC	2 cut(s)	155, 524
BsmFI	GGGAC	2 cut(s)	865, 1254
BsmI	GAATGC	1 cut(s)	1064
BsnI	GGCC	2 cut(s)	369, 523
Bso31I	GGTCTC	1 cut(s)	155
Bsp1286I	GDGCHC	3 cut(s)	314, 647, 1380
Bsp1407I	TGTACA	1 cut(s)	948
Bsp143I	GATC	5 cut(s)	200, 204, 445, 1094, 1209
BspACI	CCGC	2 cut(s)	250, 582
BspANI	GGCC	2 cut(s)	369, 523
BspCNI	CTCAG	3 cut(s)	286, 672, 970
BspDI	ATCGAT	1 cut(s)	203
BspLI	GGNNCC	6 cut(s)	28, 40, 94, 234, 669, 1008
BspMAI	CTGCAG	1 cut(s)	1423
BspMI	ACCTGC	4 cut(s)	584, 620, 1189, 1270
BspPI	GGATC	3 cut(s)	453, 1102, 1217
BspT107I	GGYRCC	2 cut(s)	232, 667
BspTI	CTTAAG	1 cut(s)	814
BspTNI	GGTCTC	1 cut(s)	155
BsrBI	CCGCTC	1 cut(s)	250
BsrDI	GCAATG	1 cut(s)	514
BsrFI	RCCGGY	3 cut(s)	175, 266, 1436
BsrGI	TGTACA	1 cut(s)	948
BsrI	ACTGG	6 cut(s)	613, 652, 1008, 1108, 1200, 1272
BssAI	RCCGGY	3 cut(s)	175, 266, 1436
BssECI	CCNNGG	4 cut(s)	33, 313, 370, 1363
BssMI	GATC	5 cut(s)	200, 204, 445, 1094, 1209
BssNI	GRCGYC	1 cut(s)	233
BssSI	CACGAG	1 cut(s)	14
Bst2BI	CACGAG	1 cut(s)	14
Bst2UI	CCWGG	5 cut(s)	315, 372, 525, 1207, 1365
Bst4CI	ACNGT	4 cut(s)	805, 875, 953, 1043
Bst6I	CTCTTC	2 cut(s)	169, 819
BstACI	GRCGYC	1 cut(s)	233
BstAFI	CTTAAG	1 cut(s)	814
BstAPI	GCANNNNNTGC	2 cut(s)	1225, 1427
BstAUI	TGTACA	1 cut(s)	948
BstBAI	YACGTR	1 cut(s)	1308
BstC8I	GCNNGC	6 cut(s)	177, 258, 521, 720, 1285, 1315
BstDEI	CTNAG	5 cut(s)	114, 294, 680, 978, 1299
BstDSI	CCRYGG	1 cut(s)	33
BstF5I	GGATG	2 cut(s)	700, 837
BstH2I	RGCGCY	1 cut(s)	236
BstHHI	GCGC	1 cut(s)	235
BstKTI	GATC	5 cut(s)	203, 207, 448, 1097, 1212
BstMAI	GTCTC	2 cut(s)	155, 524
BstMBI	GATC	5 cut(s)	200, 204, 445, 1094, 1209
BstMWI	GCNNNNNNNGC	5 cut(s)	247, 551, 667, 1225, 1427
BstNI	CCWGG	5 cut(s)	315, 372, 525, 1207, 1365
BstSCI	CCNGG	5 cut(s)	313, 370, 523, 1205, 1363
BstSFI	CTRYAG	1 cut(s)	1419
BstSLI	GKGCMC	2 cut(s)	647, 1380
BstV1I	GCAGC	8 cut(s)	143, 146, 541, 810, 1117, 1120, 1405, 1433
BstV2I	GAAGAC	1 cut(s)	1188
BstX2I	RGATCY	2 cut(s)	445, 1094
BstYI	RGATCY	2 cut(s)	445, 1094
Bsu15I	ATCGAT	1 cut(s)	203
Bsu36I	CCTNAGG	1 cut(s)	1299
BsuRI	GGCC	2 cut(s)	369, 523
BsuTUI	ATCGAT	1 cut(s)	203
BtgI	CCRYGG	1 cut(s)	33
BtrI	CACGTC	2 cut(s)	171, 361
BtsCI	GGATG	2 cut(s)	700, 837
BtsIMutI	CAGTG	4 cut(s)	645, 801, 958, 1207
BveI	ACCTGC	4 cut(s)	584, 620, 1189, 1270
Cac8I	GCNNGC	6 cut(s)	177, 258, 521, 720, 1285, 1315
CaiI	CAGNNNCTG	2 cut(s)	554, 1427
CfoI	GCGC	1 cut(s)	235
Cfr10I	RCCGGY	3 cut(s)	175, 266, 1436
Cfr13I	GGNCC	2 cut(s)	368, 1295
ClaI	ATCGAT	1 cut(s)	203
Csp6I	GTAC	9 cut(s)	427, 561, 906, 932, 949, 982, 1101, 1163, 1178
CspAI	ACCGGT	1 cut(s)	1436
CspCI	CAANNNNNGTGG	2 cut(s)	1156, 1191
CviAII	CATG	4 cut(s)	229, 356, 491, 847
CviQI	GTAC	9 cut(s)	427, 561, 906, 932, 949, 982, 1101, 1163, 1178
DdeI	CTNAG	5 cut(s)	114, 294, 680, 978, 1299
DinI	GGCGCC	1 cut(s)	234
DpnI	GATC	5 cut(s)	202, 206, 447, 1096, 1211
DpnII	GATC	5 cut(s)	200, 204, 445, 1094, 1209
Eam1104I	CTCTTC	2 cut(s)	169, 819
EarI	CTCTTC	2 cut(s)	169, 819
Eco147I	AGGCCT	1 cut(s)	523
Eco24I	GRGCYC	1 cut(s)	314
Eco31I	GGTCTC	1 cut(s)	155
Eco32I	GATATC	2 cut(s)	439, 994
Eco47I	GGWCC	1 cut(s)	1295
Eco57I	CTGAAG	1 cut(s)	531
Eco81I	CCTNAGG	1 cut(s)	1299
EcoRII	CCWGG	5 cut(s)	313, 370, 523, 1205, 1363
EcoRV	GATATC	2 cut(s)	439, 994
EcoT38I	GRGCYC	1 cut(s)	314
EgeI	GGCGCC	1 cut(s)	234
EheI	GGCGCC	1 cut(s)	234
FaeI	CATG	4 cut(s)	232, 359, 494, 850
FaqI	GGGAC	2 cut(s)	865, 1254
FatI	CATG	4 cut(s)	228, 355, 490, 846
FblI	GTMKAC	1 cut(s)	1343
Fnu4HI	GCNGC	8 cut(s)	132, 135, 555, 799, 1131, 1134, 1419, 1422
FokI	GGATG	2 cut(s)	707, 844
FriOI	GRGCYC	1 cut(s)	314
Fsp4HI	GCNGC	8 cut(s)	132, 135, 555, 799, 1131, 1134, 1419, 1422
FspBI	CTAG	2 cut(s)	285, 1148
GlaI	GCGC	1 cut(s)	234
GluI	GCNGC	8 cut(s)	132, 135, 555, 799, 1131, 1134, 1419, 1422
GsaI	CCCAGC	1 cut(s)	864
GsuI	CTGGAG	3 cut(s)	546, 606, 1025
HaeII	RGCGCY	1 cut(s)	236
HaeIII	GGCC	2 cut(s)	369, 523
HapII	CCGG	3 cut(s)	176, 267, 1437
HhaI	GCGC	1 cut(s)	235
Hin1I	GRCGYC	1 cut(s)	233
Hin1II	CATG	4 cut(s)	232, 359, 494, 850
Hin6I	GCGC	1 cut(s)	233
HinP1I	GCGC	1 cut(s)	233
HincII	GTYRAC	1 cut(s)	334
HindII	GTYRAC	1 cut(s)	334
HindIII	AAGCTT	2 cut(s)	653, 811
HinfI	GANTC	5 cut(s)	19, 384, 566, 612, 1083
HpaI	GTTAAC	1 cut(s)	334
HpaII	CCGG	3 cut(s)	176, 267, 1437
HphI	GGTGA	4 cut(s)	329, 710, 761, 1398
Hpy166II	GTNNAC	3 cut(s)	334, 949, 1344
Hpy188I	TCNGA	4 cut(s)	189, 247, 295, 681
Hpy188III	TCNNGA	5 cut(s)	16, 391, 506, 616, 1034
Hpy8I	GTNNAC	3 cut(s)	334, 949, 1344
Hpy99I	CGWCG	1 cut(s)	175
HpyAV	CCTTC	2 cut(s)	503, 1379
HpyCH4III	ACNGT	4 cut(s)	805, 875, 953, 1043
HpyCH4IV	ACGT	4 cut(s)	170, 360, 926, 1307
HpyF10VI	GCNNNNNNNGC	5 cut(s)	247, 551, 667, 1225, 1427
HpyF3I	CTNAG	5 cut(s)	114, 294, 680, 978, 1299
HpySE526I	ACGT	4 cut(s)	170, 360, 926, 1307
Hsp92I	GRCGYC	1 cut(s)	233
Hsp92II	CATG	4 cut(s)	232, 359, 494, 850
HspAI	GCGC	1 cut(s)	233
KasI	GGCGCC	1 cut(s)	232
KroI	GCCGGC	1 cut(s)	175
KroNI	GCCGGC	1 cut(s)	177
KspAI	GTTAAC	1 cut(s)	334
Kzo9I	GATC	5 cut(s)	200, 204, 445, 1094, 1209
LmnI	GCTCC	6 cut(s)	92, 227, 247, 412, 587, 1006
Lsp1109I	GCAGC	8 cut(s)	143, 146, 541, 810, 1117, 1120, 1405, 1433
LweI	GCATC	3 cut(s)	182, 616, 1433
MaeI	CTAG	2 cut(s)	285, 1148
MaeII	ACGT	4 cut(s)	170, 360, 926, 1307
MaeIII	GTNAC	4 cut(s)	317, 452, 1253, 1444
MalI	GATC	5 cut(s)	202, 206, 447, 1096, 1211
MbiI	CCGCTC	1 cut(s)	250
MboI	GATC	5 cut(s)	200, 204, 445, 1094, 1209
MboII	GAAGA	5 cut(s)	156, 286, 379, 836, 1188
MfeI	CAATTG	1 cut(s)	305
MflI	RGATCY	2 cut(s)	445, 1094
MhlI	GDGCHC	3 cut(s)	314, 647, 1380
MluCI	AATT	4 cut(s)	305, 599, 741, 1074
Mly113I	GGCGCC	1 cut(s)	233
MlyI	GAGTC	1 cut(s)	1092
MmeI	TCCRAC	1 cut(s)	698
MnlI	CCTC	9 cut(s)	83, 106, 391, 681, 822, 906, 1035, 1111, 1234
MroNI	GCCGGC	1 cut(s)	175
MseI	TTAA	3 cut(s)	333, 459, 815
MslI	CAYNNNNRTG	1 cut(s)	1333
MspA1I	CMGCKG	2 cut(s)	584, 1418
MspCI	CTTAAG	1 cut(s)	814
MspI	CCGG	3 cut(s)	176, 267, 1437
MspR9I	CCNGG	5 cut(s)	315, 372, 525, 1207, 1365
MunI	CAATTG	1 cut(s)	305
Mva1269I	GAATGC	1 cut(s)	1064
MvaI	CCWGG	5 cut(s)	315, 372, 525, 1207, 1365
MwoI	GCNNNNNNNGC	5 cut(s)	247, 551, 667, 1225, 1427
NaeI	GCCGGC	1 cut(s)	177
NarI	GGCGCC	1 cut(s)	233
NdeII	GATC	5 cut(s)	200, 204, 445, 1094, 1209
NgoMIV	GCCGGC	1 cut(s)	175
NlaIII	CATG	4 cut(s)	232, 359, 494, 850
NlaIV	GGNNCC	6 cut(s)	28, 40, 94, 234, 669, 1008
NmuCI	GTSAC	2 cut(s)	317, 1253
PaqCI	CACCTGC	2 cut(s)	584, 1270
PceI	AGGCCT	1 cut(s)	523
PctI	GAATGC	1 cut(s)	1064
PdiI	GCCGGC	1 cut(s)	177
PfeI	GAWTC	4 cut(s)	19, 384, 566, 612
PflFI	GACNNNGTC	1 cut(s)	1082
PinAI	ACCGGT	1 cut(s)	1436
PkrI	GCNGC	8 cut(s)	133, 136, 556, 800, 1132, 1135, 1420, 1423
PleI	GAGTC	1 cut(s)	1091
PluTI	GGCGCC	1 cut(s)	236
PpsI	GAGTC	1 cut(s)	1091
Ppu21I	YACGTR	1 cut(s)	1308
Psp6I	CCWGG	5 cut(s)	313, 370, 523, 1205, 1363
PspFI	CCCAGC	1 cut(s)	860
PspGI	CCWGG	5 cut(s)	313, 370, 523, 1205, 1363
PspN4I	GGNNCC	6 cut(s)	28, 40, 94, 234, 669, 1008
PspPI	GGNCC	2 cut(s)	368, 1295
PsrI	GAACNNNNNNTAC	2 cut(s)	410, 442
PstI	CTGCAG	1 cut(s)	1423
PstNI	CAGNNNCTG	2 cut(s)	554, 1427
PsuI	RGATCY	2 cut(s)	445, 1094
PsyI	GACNNNGTC	1 cut(s)	1082
PvuII	CAGCTG	1 cut(s)	1418
RsaI	GTAC	9 cut(s)	428, 562, 907, 933, 950, 983, 1102, 1164, 1179
RsaNI	GTAC	9 cut(s)	427, 561, 906, 932, 949, 982, 1101, 1163, 1178
RseI	CAYNNNNRTG	1 cut(s)	1333
SaqAI	TTAA	3 cut(s)	333, 459, 815
SatI	GCNGC	8 cut(s)	132, 135, 555, 799, 1131, 1134, 1419, 1422
Sau3AI	GATC	5 cut(s)	200, 204, 445, 1094, 1209
Sau96I	GGNCC	2 cut(s)	368, 1295
ScaI	AGTACT	1 cut(s)	428
SchI	GAGTC	1 cut(s)	1092
ScrFI	CCNGG	5 cut(s)	315, 372, 525, 1207, 1365
SduI	GDGCHC	3 cut(s)	314, 647, 1380
SfaNI	GCATC	3 cut(s)	182, 616, 1433
SfcI	CTRYAG	1 cut(s)	1419
SfoI	GGCGCC	1 cut(s)	234
SinI	GGWCC	1 cut(s)	1295
SmiMI	CAYNNNNRTG	1 cut(s)	1333
SmlI	CTYRAG	4 cut(s)	504, 662, 814, 910
SmoI	CTYRAG	4 cut(s)	504, 662, 814, 910
Sse9I	AATT	4 cut(s)	305, 599, 741, 1074
SseBI	AGGCCT	1 cut(s)	523
SsiI	CCGC	2 cut(s)	250, 582
SspDI	GGCGCC	1 cut(s)	232
SspMI	CTAG	2 cut(s)	285, 1148
StuI	AGGCCT	1 cut(s)	523
StyD4I	CCNGG	5 cut(s)	313, 370, 523, 1205, 1363
TaaI	ACNGT	4 cut(s)	805, 875, 953, 1043
TaiI	ACGT	4 cut(s)	173, 363, 929, 1310
TaqI	TCGA	2 cut(s)	203, 272
TaqII	GACCGA	1 cut(s)	1144
TasI	AATT	4 cut(s)	305, 599, 741, 1074
TatI	WGTACW	4 cut(s)	426, 948, 981, 1100
TfiI	GAWTC	4 cut(s)	19, 384, 566, 612
Tru1I	TTAA	3 cut(s)	333, 459, 815
Tru9I	TTAA	3 cut(s)	333, 459, 815
TscAI	CASTG	4 cut(s)	652, 808, 958, 1207
TseFI	GTSAC	2 cut(s)	317, 1253
TseI	GCWGC	8 cut(s)	131, 134, 554, 798, 1130, 1133, 1418, 1421
Tsp45I	GTSAC	2 cut(s)	317, 1253
TspDTI	ATGAA	4 cut(s)	171, 366, 954, 1338
TspGWI	ACGGA	2 cut(s)	214, 425
TspRI	CASTG	4 cut(s)	652, 808, 958, 1207
Tth111I	GACNNNGTC	1 cut(s)	1082
Vha464I	CTTAAG	1 cut(s)	814
VpaK11BI	GGWCC	1 cut(s)	1295
XcmI	CCANNNNNNNNNTGG	1 cut(s)	362
XmiI	GTMKAC	1 cut(s)	1343
XspI	CTAG	2 cut(s)	285, 1148
ZrmI	AGTACT	1 cut(s)	428

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.