Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0000647

Physical Location & Seq

Genomic Coordinates Reverse (-)

8663438 .. 8668736

Gene Structure 5299 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG056000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1536 bp

ATGGCTTACTCCACTCTTGCTTCACCCTCCACCTCCTTCATCAACAACACCACCAGGAAGGATTTGGGTCTCTCTGCTTTCTCTTCCACGTCGCCGGCTTCATTTCCGAGATGCAAGAGATCGGTCTCCAAGAAAATCTGCTCCGTCATGGCGCCACAGCAATCGGAGCGGAAGCCAGCCACTACTGGCTCGGTCAAGACGGGAATGACGATGACGGAGAAGATATTGGCTAGGGCTTCTGAGAAACCCCAATTGAGCCCAGGTGACAATGTTTGGGTTAACATTGATGTTTTGATGACCCATGACGTGTGTGGCCCTGGTTCATTTGGAATCTTCAAGAAAGAGTTTGGACAGAATGCAAAGGTTTGGGACCGAGAAAAGATTGTAATCATACCTGACCATTATATATTTACTACTGATGAGCGTGCAAACCGTAATGTGGATATCTTGAGGGATTTCTGCACGGAGCAGAACATCAAGTACTTCTATGATATCAAGGATCTTGGTAACTTTAAGGCTAACCCAGATTACAAGGGTGTATGCCATGTTGCTCTTGCTCAAGAAGGTCATTGCAGGCCTGGAGAGGTGCTGCTAGGTACAGATTCTCACACCTGCACCGCTGGAGCTTTTGGACAATTTGCTACTGGAATCGGGAATACTGATGCAGGTTTCGTATTGGGCACTGGGAAGCTTTTGCTCAAGGTGCCTCCAACTCTGAGATTTGTGCTGGATGGTGAAATGCCTGACTATTTGCTTGCCAAAGACTTGATTTTACAAATTATTGGTGAAATCTCTGTTGCTGGTGCAACATATAAAGCTATGGAGTTTGTTGGCAGCACTGTTGAAAGCTTAAGTATGGAAGAGAGGATGACATTATGCAACATGGTTGTTGAAGCTGGGGGTAAAAACGGTATCGTCCCTGCTGATAGCACTACATACAAGTACCTTGAGGATAAAACGTCTGTACCCTATGAACCTGTGCACAGTGATGATACAGCAAGATTTCAAACTGAGTACAGATTTGATATCTCAAAACTGGAGCCATTGGTGGCAAAGCCTCATTCTCCCGATAACCGTGCTTTAGCAAGAGAATGCAAAGATGTGAAAATTGACAGGGTCTATATTGGATCTTGTACTGGTGGGAAAACAGAGGATTTTATGGCTGCTGCTAAAGTTTTTCTAGCTTCGGGAAAAAAGGTCAAAGTACCCACTTTTCTTGTACCTGCTACACAAAAGGTTTGGATGGATGTGTACAGTCTTCCAGTGCCAGGATCAGGTGGCAAGACTTGCTCACAGATATTTGAGGAAGCTGGTTGTGACACACCTGCAAGTCCCAGTTGTGGTGCTTGCTTGGGTGGTCCTAAGGACACGTATGCTCGCTTGAATGAACCACAGGTATGTGTGTCTACTACGAACAGGAACTTCCCAGGCAGAATGGGCCACAAGGAAGGACAGATATATCTTGCTTCACCATATACAGCTGCAGCATCTGCTTTGACCGGTTATGTTACCGACCCAAGAGAGTTTTTGCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010035 GO:0010038 GO:0016143 GO:0016144 GO:0016853 GO:0016866 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044249 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0046686 GO:0050486 GO:0050896 GO:0071704 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659

KEGG Pathways

Metabolic & Signaling

ko00290 ko00660 ko00966 ko01100 ko01110 ko01210 ko01230 map00290 map00660 map00966 map01100 map01110 map01210 map01230

Pfam Domains

Protein Families

Aconitase

Protein Analysis

511

Amino Acids

55.57

Weight (kDa)

7.04

Isoelectric Point (pI)

38.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aconitase	PF00330	91 - 501	3.5e-82	Aconitase family (aconitate hydratase)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000647)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13430
fragaria_vesca	FvH4_4g04720 FvH4_4g04720 FvH4_4g04720 FvH4_4g04720 FvH4_4g04720 FvH4_5g09890
malus_domestica	MD13G1222700.v1.1 MD13G1222900.v1.1 MD16G1227200.v1.1
prunus_persica	Prupe.1G049300_v2.0.a1
pyrus_communis	pycom13g19670 pycom16g19040
rosa_chinensis	RchiOBHm_Chr4g0395521 RchiOBHm_Chr4g0395531 RchiOBHm_Chr6g0260271 RchiOBHm_Chr6g0278291
rosa_laevigata	RLG00000009126 RLG00000009614 RLG00000009616 RLG00000019332 RLG00000035454
rosa_multiflora	Rmu_co8195818.1_g000001 Rmu_co8220062.1_g000001 Rmu_sc0000041.1_g000006 Rmu_sc0000744.1_g000082 Rmu_sc0001142.1_g000007 Rmu_sc0001669.1_g000055 Rmu_sc0003400.1_g000015 Rmu_sc0005229.1_g000033 Rmu_sc0007340.1_g000006 Rmu_sc0008351.1_g000002 Rmu_sc0008351.1_g000016 Rmu_sc0039027.1_g000001
rosa_roxburghii	Rroxscaffold_4G00324270 Rroxscaffold_5G00340460 Rroxscaffold_5G00340480
rosa_rugosa	Rorug01G0023800 Rorug01G0050600 Rorug02G0280700 Rorug03G0355400 Rorug07G0181700
rosa_samantha	Rh1CG053700 Rh1DG057900 Rh3CG159300 Rh3DG159800 Rh3DG297100 Rh4AG060800 Rh4AG061300 Rh4AG061500 Rh4BG059800 Rh4BG060100 Rh4CG065500 Rh4CG065800 Rh4DG055700 Rh4DG056000 Rh5BG283600 Rh5BG284500 Rh5BG323300 Rh5BG330600 Rh5BG516600 Rh5CG076000 Rh5CG289900 Rh5DG060900 Rh5DG293300 Rh5DG293900 Rh5DG334300 Rh5DG342000 Rh6AG129800 Rh6DG223600
rosa_wichuraiana	Rw0G000910 Rw2G008950 Rw2G012250 Rw3G021630 Rw4G005010 Rw4G005020 Rw4G005030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	620, 1333
Acc36I	ACCTGC	4 cut(s)	620, 656, 1231, 1333
AccB1I	GGYRCC	2 cut(s)	151, 703
AccBSI	CCGCTC	1 cut(s)	169
AccI	GTMKAC	1 cut(s)	1406
AciI	CCGC	2 cut(s)	169, 618
AclWI	GGATC	3 cut(s)	507, 1135, 1279
AcyI	GRCGYC	1 cut(s)	152
AfaI	GTAC	9 cut(s)	482, 598, 944, 966, 1016, 1135, 1206, 1221, 1253
AfiI	CCNNNNNNNGG	4 cut(s)	439, 1268, 1274, 1340
AflII	CTTAAG	1 cut(s)	850
AflIII	ACRYGT	2 cut(s)	306, 1368
AgeI	ACCGGT	1 cut(s)	1499
AgsI	TTSAA	5 cut(s)	337, 845, 893, 1007, 1384
AjiI	CACGTC	2 cut(s)	90, 307
AjnI	CCWGG	6 cut(s)	53, 259, 316, 577, 1267, 1426
AluBI	AGCT	8 cut(s)	626, 691, 818, 849, 896, 1184, 1310, 1481
AluI	AGCT	8 cut(s)	626, 691, 818, 849, 896, 1184, 1310, 1481
Alw21I	GWGCWC	1 cut(s)	984
Alw26I	GTCTC	2 cut(s)	74, 130
Alw44I	GTGCAC	1 cut(s)	980
AlwI	GGATC	3 cut(s)	507, 1135, 1279
AlwNI	CAGNNNCTG	1 cut(s)	1490
AoxI	GGCC	3 cut(s)	313, 575, 1438
ApaLI	GTGCAC	1 cut(s)	980
ApeKI	GCWGC	6 cut(s)	589, 834, 1163, 1166, 1481, 1484
AsiGI	ACCGGT	1 cut(s)	1499
AspLEI	GCGC	1 cut(s)	154
AspS9I	GGNCC	4 cut(s)	314, 370, 1358, 1438
AsuHPI	GGTGA	5 cut(s)	15, 275, 746, 797, 1461
AvaII	GGWCC	2 cut(s)	370, 1358
AxyI	CCTNAGG	1 cut(s)	1362
BaeGI	GKGCMC	2 cut(s)	683, 984
BaeI	ACNNNNGTAYC	2 cut(s)	948, 981
BanI	GGYRCC	2 cut(s)	151, 703
BanII	GRGCYC	1 cut(s)	260
BbsI	GAAGAC	1 cut(s)	1250
Bbv12I	GWGCWC	1 cut(s)	984
BbvI	GCAGC	6 cut(s)	576, 846, 1150, 1153, 1468, 1496
BccI	CCATC	2 cut(s)	725, 1237
BcgI	CGANNNNNNTGC	2 cut(s)	1058, 1092
BciT130I	CCWGG	6 cut(s)	55, 261, 318, 579, 1269, 1428
BcoDI	GTCTC	2 cut(s)	74, 130
BfaI	CTAG	3 cut(s)	231, 593, 1181
BfmI	CTRYAG	1 cut(s)	1482
BfoI	RGCGCY	1 cut(s)	155
BfrI	CTTAAG	1 cut(s)	850
BfuAI	ACCTGC	4 cut(s)	620, 656, 1231, 1333
BisI	GCNGC	6 cut(s)	590, 835, 1164, 1167, 1482, 1485
BlsI	GCNGC	6 cut(s)	591, 836, 1165, 1168, 1483, 1486
BmcAI	AGTACT	1 cut(s)	482
Bme1390I	CCNGG	6 cut(s)	55, 261, 318, 579, 1269, 1428
Bme18I	GGWCC	2 cut(s)	370, 1358
BmgBI	CACGTC	2 cut(s)	90, 307
BmgT120I	GGNCC	4 cut(s)	314, 370, 1358, 1438
BmiI	GGNNCC	4 cut(s)	153, 371, 705, 1041
BmrFI	CCNGG	6 cut(s)	55, 261, 318, 579, 1269, 1428
BmrI	ACTGGG	2 cut(s)	693, 1329
BmsI	GCATC	3 cut(s)	101, 652, 1496
BmuI	ACTGGG	2 cut(s)	693, 1329
BpiI	GAAGAC	1 cut(s)	1250
BpmI	CTGGAG	3 cut(s)	600, 642, 1058
BpuEI	CTTGAG	4 cut(s)	469, 543, 683, 968
BsaAI	YACGTR	1 cut(s)	1371
BsaBI	GATNNNNATC	1 cut(s)	386
BsaHI	GRCGYC	1 cut(s)	152
BsaI	GGTCTC	2 cut(s)	74, 130
BsaJI	CCNNGG	3 cut(s)	259, 316, 1426
BsaWI	WCCGGW	1 cut(s)	1499
Bsc4I	CCNNNNNNNGG	4 cut(s)	439, 1268, 1274, 1340
Bse118I	RCCGGY	2 cut(s)	94, 1499
Bse1I	ACTGG	7 cut(s)	190, 649, 688, 1041, 1141, 1262, 1335
Bse21I	CCTNAGG	1 cut(s)	1362
Bse3DI	GCAATG	1 cut(s)	568
Bse8I	GATNNNNATC	1 cut(s)	386
BseBI	CCWGG	6 cut(s)	55, 261, 318, 579, 1269, 1428
BseDI	CCNNGG	3 cut(s)	259, 316, 1426
BseGI	GGATG	4 cut(s)	736, 873, 1248, 1252
BseJI	GATNNNNATC	1 cut(s)	386
BseLI	CCNNNNNNNGG	4 cut(s)	439, 1268, 1274, 1340
BseMI	GCAATG	1 cut(s)	568
BseMII	CTCAG	3 cut(s)	231, 707, 1002
BseNI	ACTGG	7 cut(s)	190, 649, 688, 1041, 1141, 1262, 1335
BseSI	GKGCMC	2 cut(s)	683, 984
BseXI	GCAGC	6 cut(s)	576, 846, 1150, 1153, 1468, 1496
BseYI	CCCAGC	1 cut(s)	896
BsgI	GTGCAG	2 cut(s)	445, 598
BshFI	GGCC	3 cut(s)	315, 577, 1440
BshNI	GGYRCC	2 cut(s)	151, 703
BshTI	ACCGGT	1 cut(s)	1499
BsiHKAI	GWGCWC	1 cut(s)	984
BsiSI	CCGG	2 cut(s)	95, 1500
BslFI	GGGAC	3 cut(s)	383, 902, 1317
BslI	CCNNNNNNNGG	4 cut(s)	439, 1268, 1274, 1340
BsmAI	GTCTC	2 cut(s)	74, 130
BsmFI	GGGAC	3 cut(s)	383, 902, 1317
BsmI	GAATGC	2 cut(s)	361, 1097
BsnI	GGCC	3 cut(s)	315, 577, 1440
Bso31I	GGTCTC	2 cut(s)	74, 130
Bsp1286I	GDGCHC	3 cut(s)	260, 683, 984
Bsp1407I	TGTACA	1 cut(s)	1251
Bsp143I	GATC	4 cut(s)	119, 499, 1127, 1271
BspACI	CCGC	2 cut(s)	169, 618
BspANI	GGCC	3 cut(s)	315, 577, 1440
BspCNI	CTCAG	3 cut(s)	232, 708, 1003
BspLI	GGNNCC	4 cut(s)	153, 371, 705, 1041
BspMAI	CTGCAG	1 cut(s)	1486
BspMI	ACCTGC	4 cut(s)	620, 656, 1231, 1333
BspPI	GGATC	3 cut(s)	507, 1135, 1279
BspT107I	GGYRCC	2 cut(s)	151, 703
BspTI	CTTAAG	1 cut(s)	850
BspTNI	GGTCTC	2 cut(s)	74, 130
BsrBI	CCGCTC	1 cut(s)	169
BsrDI	GCAATG	1 cut(s)	568
BsrFI	RCCGGY	2 cut(s)	94, 1499
BsrGI	TGTACA	1 cut(s)	1251
BsrI	ACTGG	7 cut(s)	190, 649, 688, 1041, 1141, 1262, 1335
BssAI	RCCGGY	2 cut(s)	94, 1499
BssECI	CCNNGG	3 cut(s)	259, 316, 1426
BssMI	GATC	4 cut(s)	119, 499, 1127, 1271
BssNI	GRCGYC	1 cut(s)	152
Bst2UI	CCWGG	6 cut(s)	55, 261, 318, 579, 1269, 1428
Bst4CI	ACNGT	6 cut(s)	434, 841, 911, 986, 1076, 1256
Bst6I	CTCTTC	2 cut(s)	88, 855
BstACI	GRCGYC	1 cut(s)	152
BstAFI	CTTAAG	1 cut(s)	850
BstAPI	GCANNNNNTGC	2 cut(s)	1287, 1490
BstAUI	TGTACA	1 cut(s)	1251
BstBAI	YACGTR	1 cut(s)	1371
BstC8I	GCNNGC	7 cut(s)	96, 177, 426, 575, 756, 1348, 1378
BstDEI	CTNAG	4 cut(s)	240, 716, 1011, 1362
BstF5I	GGATG	4 cut(s)	736, 873, 1248, 1252
BstH2I	RGCGCY	1 cut(s)	155
BstHHI	GCGC	1 cut(s)	154
BstKTI	GATC	4 cut(s)	122, 502, 1130, 1274
BstMAI	GTCTC	2 cut(s)	74, 130
BstMBI	GATC	4 cut(s)	119, 499, 1127, 1271
BstMWI	GCNNNNNNNGC	5 cut(s)	166, 703, 1287, 1437, 1490
BstNI	CCWGG	6 cut(s)	55, 261, 318, 579, 1269, 1428
BstSCI	CCNGG	6 cut(s)	53, 259, 316, 577, 1267, 1426
BstSFI	CTRYAG	1 cut(s)	1482
BstSLI	GKGCMC	2 cut(s)	683, 984
BstV1I	GCAGC	6 cut(s)	576, 846, 1150, 1153, 1468, 1496
BstV2I	GAAGAC	1 cut(s)	1250
BstX2I	RGATCY	2 cut(s)	499, 1127
BstYI	RGATCY	2 cut(s)	499, 1127
Bsu36I	CCTNAGG	1 cut(s)	1362
BsuRI	GGCC	3 cut(s)	315, 577, 1440
BtrI	CACGTC	2 cut(s)	90, 307
BtsCI	GGATG	4 cut(s)	736, 873, 1248, 1252
BtsIMutI	CAGTG	4 cut(s)	681, 837, 991, 1269
BveI	ACCTGC	4 cut(s)	620, 656, 1231, 1333
Cac8I	GCNNGC	7 cut(s)	96, 177, 426, 575, 756, 1348, 1378
CaiI	CAGNNNCTG	1 cut(s)	1490
CfoI	GCGC	1 cut(s)	154
Cfr10I	RCCGGY	2 cut(s)	94, 1499
Cfr13I	GGNCC	4 cut(s)	314, 370, 1358, 1438
Csp6I	GTAC	9 cut(s)	481, 597, 943, 965, 1015, 1134, 1205, 1220, 1252
CspAI	ACCGGT	1 cut(s)	1499
CspCI	CAANNNNNGTGG	2 cut(s)	1198, 1233
CviAII	CATG	4 cut(s)	148, 302, 545, 883
CviQI	GTAC	9 cut(s)	481, 597, 943, 965, 1015, 1134, 1205, 1220, 1252
DdeI	CTNAG	4 cut(s)	240, 716, 1011, 1362
DinI	GGCGCC	1 cut(s)	153
DpnI	GATC	4 cut(s)	121, 501, 1129, 1273
DpnII	GATC	4 cut(s)	119, 499, 1127, 1271
Eam1104I	CTCTTC	2 cut(s)	88, 855
EarI	CTCTTC	2 cut(s)	88, 855
Eco147I	AGGCCT	1 cut(s)	577
Eco24I	GRGCYC	1 cut(s)	260
Eco31I	GGTCTC	2 cut(s)	74, 130
Eco32I	GATATC	3 cut(s)	445, 493, 1027
Eco47I	GGWCC	2 cut(s)	370, 1358
Eco81I	CCTNAGG	1 cut(s)	1362
EcoRII	CCWGG	6 cut(s)	53, 259, 316, 577, 1267, 1426
EcoRV	GATATC	3 cut(s)	445, 493, 1027
EcoT38I	GRGCYC	1 cut(s)	260
EgeI	GGCGCC	1 cut(s)	153
EheI	GGCGCC	1 cut(s)	153
FaeI	CATG	4 cut(s)	151, 305, 548, 886
FaqI	GGGAC	3 cut(s)	383, 902, 1317
FatI	CATG	4 cut(s)	147, 301, 544, 882
FblI	GTMKAC	1 cut(s)	1406
Fnu4HI	GCNGC	6 cut(s)	590, 835, 1164, 1167, 1482, 1485
FokI	GGATG	4 cut(s)	743, 880, 1255, 1259
FriOI	GRGCYC	1 cut(s)	260
Fsp4HI	GCNGC	6 cut(s)	590, 835, 1164, 1167, 1482, 1485
FspBI	CTAG	3 cut(s)	231, 593, 1181
GlaI	GCGC	1 cut(s)	153
GluI	GCNGC	6 cut(s)	590, 835, 1164, 1167, 1482, 1485
GsaI	CCCAGC	1 cut(s)	900
GsuI	CTGGAG	3 cut(s)	600, 642, 1058
HaeII	RGCGCY	1 cut(s)	155
HaeIII	GGCC	3 cut(s)	315, 577, 1440
HapII	CCGG	2 cut(s)	95, 1500
HhaI	GCGC	1 cut(s)	154
Hin1I	GRCGYC	1 cut(s)	152
Hin1II	CATG	4 cut(s)	151, 305, 548, 886
Hin6I	GCGC	1 cut(s)	152
HinP1I	GCGC	1 cut(s)	152
HincII	GTYRAC	1 cut(s)	280
HindII	GTYRAC	1 cut(s)	280
HindIII	AAGCTT	2 cut(s)	689, 847
HinfI	GANTC	3 cut(s)	330, 602, 648
HpaI	GTTAAC	1 cut(s)	280
HpaII	CCGG	2 cut(s)	95, 1500
HphI	GGTGA	5 cut(s)	15, 275, 746, 797, 1461
Hpy166II	GTNNAC	4 cut(s)	280, 982, 1252, 1407
Hpy188I	TCNGA	4 cut(s)	108, 166, 241, 717
Hpy188III	TCNNGA	7 cut(s)	196, 337, 448, 560, 652, 1067, 1188
Hpy8I	GTNNAC	4 cut(s)	280, 982, 1252, 1407
Hpy99I	CGWCG	1 cut(s)	94
HpyAV	CCTTC	4 cut(s)	46, 52, 557, 1442
HpyCH4III	ACNGT	6 cut(s)	434, 841, 911, 986, 1076, 1256
HpyCH4IV	ACGT	4 cut(s)	89, 306, 959, 1370
HpyF10VI	GCNNNNNNNGC	5 cut(s)	166, 703, 1287, 1437, 1490
HpyF3I	CTNAG	4 cut(s)	240, 716, 1011, 1362
HpySE526I	ACGT	4 cut(s)	89, 306, 959, 1370
Hsp92I	GRCGYC	1 cut(s)	152
Hsp92II	CATG	4 cut(s)	151, 305, 548, 886
HspAI	GCGC	1 cut(s)	152
KasI	GGCGCC	1 cut(s)	151
KroI	GCCGGC	1 cut(s)	94
KroNI	GCCGGC	1 cut(s)	96
KspAI	GTTAAC	1 cut(s)	280
Kzo9I	GATC	4 cut(s)	119, 499, 1127, 1271
LmnI	GCTCC	5 cut(s)	146, 166, 466, 623, 1039
Lsp1109I	GCAGC	6 cut(s)	576, 846, 1150, 1153, 1468, 1496
LweI	GCATC	3 cut(s)	101, 652, 1496
MaeI	CTAG	3 cut(s)	231, 593, 1181
MaeII	ACGT	4 cut(s)	89, 306, 959, 1370
MaeIII	GTNAC	4 cut(s)	263, 506, 1316, 1507
MalI	GATC	4 cut(s)	121, 501, 1129, 1273
MbiI	CCGCTC	1 cut(s)	169
MboI	GATC	4 cut(s)	119, 499, 1127, 1271
MboII	GAAGA	5 cut(s)	75, 232, 325, 872, 1250
MfeI	CAATTG	1 cut(s)	251
MflI	RGATCY	2 cut(s)	499, 1127
MhlI	GDGCHC	3 cut(s)	260, 683, 984
MluCI	AATT	4 cut(s)	251, 635, 777, 1107
Mly113I	GGCGCC	1 cut(s)	152
MmeI	TCCRAC	1 cut(s)	734
MroNI	GCCGGC	1 cut(s)	94
MseI	TTAA	3 cut(s)	279, 513, 851
MslI	CAYNNNNRTG	2 cut(s)	987, 1396
MspA1I	CMGCKG	2 cut(s)	620, 1481
MspCI	CTTAAG	1 cut(s)	850
MspI	CCGG	2 cut(s)	95, 1500
MspR9I	CCNGG	6 cut(s)	55, 261, 318, 579, 1269, 1428
MunI	CAATTG	1 cut(s)	251
Mva1269I	GAATGC	2 cut(s)	361, 1097
MvaI	CCWGG	6 cut(s)	55, 261, 318, 579, 1269, 1428
MwoI	GCNNNNNNNGC	5 cut(s)	166, 703, 1287, 1437, 1490
NaeI	GCCGGC	1 cut(s)	96
NarI	GGCGCC	1 cut(s)	152
NdeII	GATC	4 cut(s)	119, 499, 1127, 1271
NgoMIV	GCCGGC	1 cut(s)	94
NlaIII	CATG	4 cut(s)	151, 305, 548, 886
NlaIV	GGNNCC	4 cut(s)	153, 371, 705, 1041
NmuCI	GTSAC	2 cut(s)	263, 1316
PaqCI	CACCTGC	2 cut(s)	620, 1333
PceI	AGGCCT	1 cut(s)	577
PcsI	WCGNNNNNNNCGW	1 cut(s)	206
PctI	GAATGC	2 cut(s)	361, 1097
PdiI	GCCGGC	1 cut(s)	96
PfeI	GAWTC	3 cut(s)	330, 602, 648
PflFI	GACNNNGTC	1 cut(s)	1115
PinAI	ACCGGT	1 cut(s)	1499
PkrI	GCNGC	6 cut(s)	591, 836, 1165, 1168, 1483, 1486
PluTI	GGCGCC	1 cut(s)	155
Ppu21I	YACGTR	1 cut(s)	1371
Psp6I	CCWGG	6 cut(s)	53, 259, 316, 577, 1267, 1426
PspFI	CCCAGC	1 cut(s)	896
PspGI	CCWGG	6 cut(s)	53, 259, 316, 577, 1267, 1426
PspN4I	GGNNCC	4 cut(s)	153, 371, 705, 1041
PspPI	GGNCC	4 cut(s)	314, 370, 1358, 1438
PsrI	GAACNNNNNNTAC	2 cut(s)	464, 496
PstI	CTGCAG	1 cut(s)	1486
PstNI	CAGNNNCTG	1 cut(s)	1490
PsuI	RGATCY	2 cut(s)	499, 1127
PsyI	GACNNNGTC	1 cut(s)	1115
PvuII	CAGCTG	1 cut(s)	1481
RsaI	GTAC	9 cut(s)	482, 598, 944, 966, 1016, 1135, 1206, 1221, 1253
RsaNI	GTAC	9 cut(s)	481, 597, 943, 965, 1015, 1134, 1205, 1220, 1252
RseI	CAYNNNNRTG	2 cut(s)	987, 1396
SaqAI	TTAA	3 cut(s)	279, 513, 851
SatI	GCNGC	6 cut(s)	590, 835, 1164, 1167, 1482, 1485
Sau3AI	GATC	4 cut(s)	119, 499, 1127, 1271
Sau96I	GGNCC	4 cut(s)	314, 370, 1358, 1438
ScaI	AGTACT	1 cut(s)	482
ScrFI	CCNGG	6 cut(s)	55, 261, 318, 579, 1269, 1428
SduI	GDGCHC	3 cut(s)	260, 683, 984
SfaNI	GCATC	3 cut(s)	101, 652, 1496
SfcI	CTRYAG	1 cut(s)	1482
SfoI	GGCGCC	1 cut(s)	153
SinI	GGWCC	2 cut(s)	370, 1358
SmiMI	CAYNNNNRTG	2 cut(s)	987, 1396
SmlI	CTYRAG	5 cut(s)	448, 558, 698, 850, 947
SmoI	CTYRAG	5 cut(s)	448, 558, 698, 850, 947
Sse9I	AATT	4 cut(s)	251, 635, 777, 1107
SseBI	AGGCCT	1 cut(s)	577
SsiI	CCGC	2 cut(s)	169, 618
SspDI	GGCGCC	1 cut(s)	151
SspMI	CTAG	3 cut(s)	231, 593, 1181
StuI	AGGCCT	1 cut(s)	577
StyD4I	CCNGG	6 cut(s)	53, 259, 316, 577, 1267, 1426
TaaI	ACNGT	6 cut(s)	434, 841, 911, 986, 1076, 1256
TaiI	ACGT	4 cut(s)	92, 309, 962, 1373
TaqII	GACCGA	3 cut(s)	112, 181, 387
TasI	AATT	4 cut(s)	251, 635, 777, 1107
TatI	WGTACW	4 cut(s)	480, 1014, 1133, 1251
TfiI	GAWTC	3 cut(s)	330, 602, 648
Tru1I	TTAA	3 cut(s)	279, 513, 851
Tru9I	TTAA	3 cut(s)	279, 513, 851
TscAI	CASTG	4 cut(s)	688, 844, 991, 1269
TseFI	GTSAC	2 cut(s)	263, 1316
TseI	GCWGC	6 cut(s)	589, 834, 1163, 1166, 1481, 1484
Tsp45I	GTSAC	2 cut(s)	263, 1316
TspDTI	ATGAA	5 cut(s)	28, 90, 312, 987, 1401
TspGWI	ACGGA	3 cut(s)	133, 230, 479
TspRI	CASTG	4 cut(s)	688, 844, 991, 1269
Tth111I	GACNNNGTC	1 cut(s)	1115
Vha464I	CTTAAG	1 cut(s)	850
VneI	GTGCAC	1 cut(s)	980
VpaK11BI	GGWCC	2 cut(s)	370, 1358
XcmI	CCANNNNNNNNNTGG	2 cut(s)	61, 308
XmiI	GTMKAC	1 cut(s)	1406
XspI	CTAG	3 cut(s)	231, 593, 1181
ZrmI	AGTACT	1 cut(s)	482

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.