Rh5BG516600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000647
Physical Location & Seq
Forward (+)
81816355 .. 81817045
691 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG516600.1

Sequence Viewer

Length: 294 bp
ATGGCTGACGGAGTCGACCATAATGCCAAGAAGCACCGCGCGACGGCGTCGGGGCCTCTTCGATCATGGAGCTCAAAGCCTAGCTTTACCAATCTCAAGAAGATTCCAAGAAGTACAAAGACCTCGCAGACCCCGACGCCGAGTTCCACCGCGCTAAGTAGAAGATCACCGCTCACAATGTCTTCTCTGCCAAGAACTCCGGCATCAACGCCTGGGCCCACAGGCGCCACTAAATCTGGTGGAAAACATGTTGAGTTTGTTATCCATTTGGGTAGAACACCAAACACTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

97

Amino Acids

10.22

Weight (kDa)

11.9

Isoelectric Point (pI)

52.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 224
AccBSI CCGCTC 1 cut(s) 172
AccI GTMKAC 1 cut(s) 15
AccII CGCG 3 cut(s) 39, 41, 152
AciI CCGC 3 cut(s) 37, 150, 170
AcyI GRCGYC 3 cut(s) 47, 137, 225
AfaI GTAC 1 cut(s) 115
AfiI CCNNNNNNNGG 1 cut(s) 43
AflIII ACRYGT 1 cut(s) 247
AjnI CCWGG 1 cut(s) 211
AluBI AGCT 2 cut(s) 72, 84
AluI AGCT 2 cut(s) 72, 84
Alw21I GWGCWC 1 cut(s) 74
AoxI GGCC 2 cut(s) 53, 215
ApaI GGGCCC 1 cut(s) 219
AspLEI GCGC 3 cut(s) 41, 154, 227
AspS9I GGNCC 3 cut(s) 53, 215, 216
AsuHPI GGTGA 1 cut(s) 159
BaeGI GKGCMC 1 cut(s) 219
BanI GGYRCC 1 cut(s) 224
BanII GRGCYC 2 cut(s) 74, 219
BbsI GAAGAC 1 cut(s) 174
Bbv12I GWGCWC 1 cut(s) 74
BceAI ACGGC 1 cut(s) 60
BcgI CGANNNNNNTGC 2 cut(s) 5, 39
BciT130I CCWGG 1 cut(s) 213
BfaI CTAG 1 cut(s) 81
BfoI RGCGCY 1 cut(s) 228
Bme1390I CCNGG 1 cut(s) 213
BmgT120I GGNCC 3 cut(s) 53, 215, 216
BmiI GGNNCC 3 cut(s) 54, 217, 226
BmrFI CCNGG 1 cut(s) 213
BmsI GCATC 1 cut(s) 212
BpiI GAAGAC 1 cut(s) 174
BpuEI CTTGAG 1 cut(s) 80
BsaHI GRCGYC 3 cut(s) 47, 137, 225
BsaJI CCNNGG 1 cut(s) 212
Bsc4I CCNNNNNNNGG 1 cut(s) 43
BseBI CCWGG 1 cut(s) 213
BseDI CCNNGG 1 cut(s) 212
BseLI CCNNNNNNNGG 1 cut(s) 43
BseSI GKGCMC 1 cut(s) 219
Bsh1236I CGCG 3 cut(s) 39, 41, 152
BshFI GGCC 2 cut(s) 55, 217
BshNI GGYRCC 1 cut(s) 224
BsiHKAI GWGCWC 1 cut(s) 74
BsiSI CCGG 1 cut(s) 200
BslI CCNNNNNNNGG 1 cut(s) 43
BsnI GGCC 2 cut(s) 55, 217
Bsp120I GGGCCC 1 cut(s) 215
Bsp1286I GDGCHC 2 cut(s) 74, 219
Bsp143I GATC 2 cut(s) 62, 164
BspACI CCGC 3 cut(s) 37, 150, 170
BspANI GGCC 2 cut(s) 55, 217
BspFNI CGCG 3 cut(s) 39, 41, 152
BspLI GGNNCC 3 cut(s) 54, 217, 226
BspT107I GGYRCC 1 cut(s) 224
BsrBI CCGCTC 1 cut(s) 172
BssECI CCNNGG 1 cut(s) 212
BssMI GATC 2 cut(s) 62, 164
BssNI GRCGYC 3 cut(s) 47, 137, 225
Bst2UI CCWGG 1 cut(s) 213
Bst6I CTCTTC 1 cut(s) 63
BstACI GRCGYC 3 cut(s) 47, 137, 225
BstDEI CTNAG 1 cut(s) 155
BstFNI CGCG 3 cut(s) 39, 41, 152
BstH2I RGCGCY 1 cut(s) 228
BstHHI GCGC 3 cut(s) 41, 154, 227
BstKTI GATC 2 cut(s) 65, 167
BstMBI GATC 2 cut(s) 62, 164
BstNI CCWGG 1 cut(s) 213
BstNSI RCATGY 1 cut(s) 251
BstSCI CCNGG 1 cut(s) 211
BstSLI GKGCMC 1 cut(s) 219
BstUI CGCG 3 cut(s) 39, 41, 152
BstV2I GAAGAC 1 cut(s) 174
BsuRI GGCC 2 cut(s) 55, 217
BtsI GCAGTG 1 cut(s) 285
BtsIMutI CAGTG 1 cut(s) 285
CfoI GCGC 3 cut(s) 41, 154, 227
Cfr13I GGNCC 3 cut(s) 53, 215, 216
CseI GACGC 2 cut(s) 36, 145
Csp6I GTAC 1 cut(s) 114
CviAII CATG 3 cut(s) 66, 248, 291
CviJI RGCY 6 cut(s) 5, 55, 72, 79, 84, 217
CviKI_1 RGCY 6 cut(s) 5, 55, 72, 79, 84, 217
CviQI GTAC 1 cut(s) 114
DdeI CTNAG 1 cut(s) 155
DinI GGCGCC 1 cut(s) 226
DpnI GATC 2 cut(s) 64, 166
DpnII GATC 2 cut(s) 62, 164
Eam1104I CTCTTC 1 cut(s) 63
EarI CTCTTC 1 cut(s) 63
Ecl136II GAGCTC 1 cut(s) 72
Eco24I GRGCYC 2 cut(s) 74, 219
Eco53kI GAGCTC 1 cut(s) 72
EcoICRI GAGCTC 1 cut(s) 72
EcoO109I RGGNCCY 1 cut(s) 53
EcoRII CCWGG 1 cut(s) 211
EcoT38I GRGCYC 2 cut(s) 74, 219
EgeI GGCGCC 1 cut(s) 226
EheI GGCGCC 1 cut(s) 226
FaeI CATG 3 cut(s) 69, 251, 294
FaiI YATR 4 cut(s) 21, 67, 249, 292
FalI AAGNNNNNCTT 2 cut(s) 68, 100
FatI CATG 3 cut(s) 65, 247, 290
FblI GTMKAC 1 cut(s) 15
FriOI GRGCYC 2 cut(s) 74, 219
FspBI CTAG 1 cut(s) 81
GlaI GCGC 3 cut(s) 40, 153, 226
HaeII RGCGCY 1 cut(s) 228
HaeIII GGCC 2 cut(s) 55, 217
HapII CCGG 1 cut(s) 200
HgaI GACGC 2 cut(s) 36, 145
HhaI GCGC 3 cut(s) 41, 154, 227
Hin1I GRCGYC 3 cut(s) 47, 137, 225
Hin1II CATG 3 cut(s) 69, 251, 294
Hin6I GCGC 3 cut(s) 39, 152, 225
HinP1I GCGC 3 cut(s) 39, 152, 225
HincII GTYRAC 1 cut(s) 16
HindII GTYRAC 1 cut(s) 16
HinfI GANTC 2 cut(s) 12, 103
HpaII CCGG 1 cut(s) 200
HphI GGTGA 1 cut(s) 159
Hpy166II GTNNAC 1 cut(s) 16
Hpy188III TCNNGA 1 cut(s) 97
Hpy8I GTNNAC 1 cut(s) 16
Hpy99I CGWCG 3 cut(s) 46, 52, 139
HpyCH4V TGCA 1 cut(s) 290
HpyF3I CTNAG 1 cut(s) 155
Hsp92I GRCGYC 3 cut(s) 47, 137, 225
Hsp92II CATG 3 cut(s) 69, 251, 294
HspAI GCGC 3 cut(s) 39, 152, 225
KasI GGCGCC 1 cut(s) 224
Kzo9I GATC 2 cut(s) 62, 164
LmnI GCTCC 1 cut(s) 69
LpnPI CCDG 5 cut(s) 198, 207, 213, 222, 225
LweI GCATC 1 cut(s) 212
MaeI CTAG 1 cut(s) 81
MalI GATC 2 cut(s) 64, 166
MbiI CCGCTC 1 cut(s) 172
MboI GATC 2 cut(s) 62, 164
MboII GAAGA 4 cut(s) 50, 112, 174, 174
MhlI GDGCHC 2 cut(s) 74, 219
Mly113I GGCGCC 1 cut(s) 225
MlyI GAGTC 1 cut(s) 21
MnlI CCTC 2 cut(s) 66, 133
MspI CCGG 1 cut(s) 200
MspR9I CCNGG 1 cut(s) 213
MvaI CCWGG 1 cut(s) 213
MvnI CGCG 3 cut(s) 39, 41, 152
NarI GGCGCC 1 cut(s) 225
NdeII GATC 2 cut(s) 62, 164
NlaIII CATG 3 cut(s) 69, 251, 294
NlaIV GGNNCC 3 cut(s) 54, 217, 226
NmeAIII GCCGAG 1 cut(s) 165
NspI RCATGY 1 cut(s) 251
PciI ACATGT 1 cut(s) 247
PcsI WCGNNNNNNNCGW 1 cut(s) 131
PfeI GAWTC 1 cut(s) 103
PflFI GACNNNGTC 2 cut(s) 11, 46
PleI GAGTC 1 cut(s) 20
PluTI GGCGCC 1 cut(s) 228
PpsI GAGTC 1 cut(s) 20
PscI ACATGT 1 cut(s) 247
Psp124BI GAGCTC 1 cut(s) 74
Psp6I CCWGG 1 cut(s) 211
PspGI CCWGG 1 cut(s) 211
PspN4I GGNNCC 3 cut(s) 54, 217, 226
PspOMI GGGCCC 1 cut(s) 215
PspPI GGNCC 3 cut(s) 53, 215, 216
PsyI GACNNNGTC 2 cut(s) 11, 46
RsaI GTAC 1 cut(s) 115
RsaNI GTAC 1 cut(s) 114
SacI GAGCTC 1 cut(s) 74
SalI GTCGAC 1 cut(s) 14
Sau3AI GATC 2 cut(s) 62, 164
Sau96I GGNCC 3 cut(s) 53, 215, 216
SchI GAGTC 1 cut(s) 21
ScrFI CCNGG 1 cut(s) 213
SduI GDGCHC 2 cut(s) 74, 219
SetI ASST 3 cut(s) 74, 86, 125
SfaNI GCATC 1 cut(s) 212
SfoI GGCGCC 1 cut(s) 226
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
SsiI CCGC 3 cut(s) 37, 150, 170
SspDI GGCGCC 1 cut(s) 224
SspMI CTAG 1 cut(s) 81
SstI GAGCTC 1 cut(s) 74
StyD4I CCNGG 1 cut(s) 211
TaqI TCGA 2 cut(s) 15, 61
TatI WGTACW 1 cut(s) 113
TfiI GAWTC 1 cut(s) 103
TscAI CASTG 1 cut(s) 292
TspGWI ACGGA 1 cut(s) 24
TspRI CASTG 1 cut(s) 292
Tth111I GACNNNGTC 2 cut(s) 11, 46
XceI RCATGY 1 cut(s) 251
XmiI GTMKAC 1 cut(s) 15
XspI CTAG 1 cut(s) 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.