AT3G28880

OST-HTH/LOTUS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000679
Physical Location & Seq
Forward (+)
10893616 .. 10895895
2280 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G28880.1

Sequence Viewer

Length: 1479 bp
ATGGTTCTGCTTAGAGAGATTGAGCTTCTTCAGCTCTTTGGTGCTGCAGTTTTTAGTGAATCTGTGGATAAGCAAACTTCACCACTTATCTCCATTGTGCAGGCAGGAGATGAAGCTGTTCTTGAGCTGTTTATAAACACCAACTTCGATGTCAACGAGAAAAATATAGAAGGGAATACTGTTCTTCAATGTAGCCTGAAAGGATCGTCTGTGCCACATAACCAGCAAACCAGAATCATGAACCTACTTATTGCACACGGTGCTCGAGTTAACCAAAAGAACAAGTTGGGTTTATCTGCAGTTCACTTTGCTGCTGCAAATGGCAATTTATCTACACTTGAGATTCTACTAGCGGCCAACCCTGACTTGGTTAACATGAAGACGGTAATCAAAGAGACGCCACTATTCTTCGCTGTGAAGAATAATCATCTTGACTGCGTGGAGCTTCTTCTTCGATGTGGTGCAATTACGGAGATTCATAACCTACGCAAAGAAACAGAACTTGCACAGTCACAAAGTGCAGCAGTTTCACCAAATGCAAGTGATGAGGAAACTCTTGGATTTCTCGGAGATTCTGTTCCAACATGGCTCAAAACATTTGTAACCTGGCTTCCTCTGTACCTCCGAGATACATCAGCGAACTGGAAAGGGGCTGGATATCCCCTGTCATCCTTCAAAGCCGATTTTCGTGCTGTTTTTGGAATGGATCTCGATCACACCTCTCTTGATTTCCCTAAGCACATCGACTTTGTCAAATACTTTCCAAGACTCTGTCAAATGAAAGTCGTCCCCATAGGTAAACTTGGAGCTGCAACACACTGGGTCATGCTACCCACCAAATCTAAGTGTTCTCAGCTCAAAGAAAGACCTCCTGAGCCACTCATTATCACTAAGAATGACTCTGTTGCTTCTCCCAGCAAACCAAAAGCCTTATCAGTTCCTCCAGATTTTAAATTCATACAAGAAGCACATGACTCTAAAACCTTCAAAGCTTCACCACAACCAAAAGCTCAACCTCCTCCTCCCTTAACAGCATCGTACAACAACAACCAACGTCAACTAAAACACCCGGTTCTTGAGACACTTACAAAGATCAGAAACAGCACTTCTGTCTTCTTTCTCCGTGAGTTTGACTTTTATCAAAGCTATGAGACATGTTTAAAGGAAGGCATGTGTTTCTGGTGCAACAAGAACATGATTCGATGGGCAAACTTCCCTTGCAGACACAAACTTTGGTGCAGCTCTTGTAAACAACAGATTACTCAATCTACTTCAGGAGAGAAGATCCTTGAGGAAGACCATCATAAGTGTGTGGTCTGTGATGCAAAAGTGGAACGTTTCGTCTTGTCTCCACCGTTTGAATCCGGTCACCATAGGATTTTAAGTGATCGCCCTAACAACGCCGAATTAGCCACTTCTTTCCTTCAGTTCCTCAGCATAAGGAACTCCATGAACAAGATGATATATCGCGGGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 OST-HTH RRM_1

Protein Analysis

492

Amino Acids

55.33

Weight (kDa)

8.67

Isoelectric Point (pI)

43.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 75 - 155 3.6e-13 Ankyrin repeats (3 copies)
OST-HTH PF12872 196 - 267 5.3e-08 OST-HTH/LOTUS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 134
AccII CGCG 1 cut(s) 1470
AciI CCGC 2 cut(s) 353, 1470
AclI AACGTT 1 cut(s) 1336
AclWI GGATC 3 cut(s) 211, 714, 1279
AcoI YGGCCR 1 cut(s) 354
AcsI RAATTY 1 cut(s) 953
AcuI CTGAAG 3 cut(s) 14, 1257, 1409
AcyI GRCGYC 1 cut(s) 398
AdeI CACNNNGTG 2 cut(s) 260, 518
AfaI GTAC 2 cut(s) 620, 1040
AfiI CCNNNNNNNGG 1 cut(s) 367
AflIII ACRYGT 1 cut(s) 1154
AgsI TTSAA 4 cut(s) 188, 676, 988, 1361
AjnI CCWGG 1 cut(s) 605
AloI GAACNNNNNNTCC 2 cut(s) 561, 593
Alw21I GWGCWC 1 cut(s) 265
Alw26I GTCTC 4 cut(s) 389, 1073, 1145, 1353
AlwI GGATC 3 cut(s) 211, 714, 1279
Ama87I CYCGRG 1 cut(s) 264
AoxI GGCC 1 cut(s) 354
ApeKI GCWGC 6 cut(s) 44, 311, 314, 521, 809, 1239
ApoI RAATTY 1 cut(s) 953
ArsI GACNNNNNNTTYG 2 cut(s) 1215, 1247
Asp700I GAANNNNTTC 1 cut(s) 117
AsuC2I CCSGG 1 cut(s) 1070
AsuHPI GGTGA 4 cut(s) 72, 522, 987, 1361
AvaI CYCGRG 1 cut(s) 264
BarI GAAGNNNNNNTAC 2 cut(s) 594, 626
BbsI GAAGAC 3 cut(s) 386, 1105, 1302
Bbv12I GWGCWC 1 cut(s) 265
BbvCI CCTCAGC 1 cut(s) 1433
BbvI GCAGC 6 cut(s) 31, 298, 301, 533, 796, 1251
BccI CCATC 2 cut(s) 1197, 1308
BcgI CGANNNNNNTGC 2 cut(s) 671, 705
BciT130I CCWGG 1 cut(s) 607
BcnI CCSGG 1 cut(s) 1070
BcoDI GTCTC 4 cut(s) 389, 1073, 1145, 1353
BfaI CTAG 1 cut(s) 350
BfmI CTRYAG 2 cut(s) 45, 297
BisI GCNGC 7 cut(s) 45, 312, 315, 354, 522, 810, 1240
BlsI GCNGC 7 cut(s) 46, 313, 316, 355, 523, 811, 1241
Bme1390I CCNGG 2 cut(s) 607, 1070
BmeT110I CYCGRG 1 cut(s) 264
BmrFI CCNGG 2 cut(s) 607, 1070
BmrI ACTGGG 1 cut(s) 829
BmsI GCATC 2 cut(s) 1043, 1312
BmuI ACTGGG 1 cut(s) 829
BpiI GAAGAC 3 cut(s) 386, 1105, 1302
BpmI CTGGAG 1 cut(s) 927
Bpu10I CCTNAGC 3 cut(s) 735, 873, 1433
BpuEI CTTGAG 4 cut(s) 143, 359, 1097, 1310
BpuMI CCSGG 1 cut(s) 1070
BsaBI GATNNNNATC 1 cut(s) 711
BsaHI GRCGYC 1 cut(s) 398
BsaWI WCCGGW 1 cut(s) 1364
BsaXI ACNNNNNCTCC 2 cut(s) 561, 591
Bsc4I CCNNNNNNNGG 1 cut(s) 367
Bse1I ACTGG 2 cut(s) 647, 824
Bse8I GATNNNNATC 1 cut(s) 711
BseBI CCWGG 1 cut(s) 607
BseGI GGATG 1 cut(s) 668
BseJI GATNNNNATC 1 cut(s) 711
BseLI CCNNNNNNNGG 1 cut(s) 367
BseMII CTCAG 3 cut(s) 864, 866, 1447
BseNI ACTGG 2 cut(s) 647, 824
BseRI GAGGAG 2 cut(s) 1008, 1011
BseXI GCAGC 6 cut(s) 31, 298, 301, 533, 796, 1251
BseYI CCCAGC 1 cut(s) 914
BsgI GTGCAG 3 cut(s) 119, 540, 1258
Bsh1236I CGCG 1 cut(s) 1470
BshFI GGCC 1 cut(s) 356
BsiHKAI GWGCWC 1 cut(s) 265
BsiHKCI CYCGRG 1 cut(s) 264
BsiSI CCGG 2 cut(s) 1070, 1365
BslFI GGGAC 1 cut(s) 773
BslI CCNNNNNNNGG 1 cut(s) 367
BsmAI GTCTC 4 cut(s) 389, 1073, 1145, 1353
BsmBI CGTCTC 1 cut(s) 389
BsmFI GGGAC 1 cut(s) 773
BsnI GGCC 1 cut(s) 356
BsoBI CYCGRG 1 cut(s) 264
Bsp1286I GDGCHC 1 cut(s) 265
Bsp143I GATC 6 cut(s) 203, 706, 712, 1092, 1284, 1387
BspACI CCGC 2 cut(s) 353, 1470
BspANI GGCC 1 cut(s) 356
BspCNI CTCAG 3 cut(s) 865, 865, 1446
BspFNI CGCG 1 cut(s) 1470
BspHI TCATGA 1 cut(s) 237
BspMAI CTGCAG 2 cut(s) 49, 301
BspPI GGATC 3 cut(s) 211, 714, 1279
BsrI ACTGG 2 cut(s) 647, 824
BssMI GATC 6 cut(s) 203, 706, 712, 1092, 1284, 1387
BssNI GRCGYC 1 cut(s) 398
Bst2UI CCWGG 1 cut(s) 607
Bst4CI ACNGT 5 cut(s) 181, 260, 385, 510, 1356
BstACI GRCGYC 1 cut(s) 398
BstAPI GCANNNNNTGC 1 cut(s) 260
BstC8I GCNNGC 1 cut(s) 102
BstDEI CTNAG 7 cut(s) 11, 735, 843, 852, 873, 891, 1433
BstEII GGTNACC 1 cut(s) 1367
BstF5I GGATG 1 cut(s) 668
BstFNI CGCG 1 cut(s) 1470
BstKTI GATC 6 cut(s) 206, 709, 715, 1095, 1287, 1390
BstMAI GTCTC 4 cut(s) 389, 1073, 1145, 1353
BstMBI GATC 6 cut(s) 203, 706, 712, 1092, 1284, 1387
BstMWI GCNNNNNNNGC 3 cut(s) 31, 260, 1409
BstNI CCWGG 1 cut(s) 607
BstNSI RCATGY 2 cut(s) 1158, 1174
BstPI GGTNACC 1 cut(s) 1367
BstSCI CCNGG 2 cut(s) 605, 1068
BstSFI CTRYAG 2 cut(s) 45, 297
BstUI CGCG 1 cut(s) 1470
BstV1I GCAGC 6 cut(s) 31, 298, 301, 533, 796, 1251
BstV2I GAAGAC 3 cut(s) 386, 1105, 1302
BstX2I RGATCY 2 cut(s) 706, 1284
BstYI RGATCY 2 cut(s) 706, 1284
BsuRI GGCC 1 cut(s) 356
BtsCI GGATG 1 cut(s) 668
BtsIMutI CAGTG 1 cut(s) 817
Cac8I GCNNGC 1 cut(s) 102
CciI TCATGA 1 cut(s) 237
CseI GACGC 1 cut(s) 406
Csp6I GTAC 2 cut(s) 619, 1039
CviAII CATG 9 cut(s) 238, 376, 585, 826, 971, 1155, 1171, 1195, 1450
CviQI GTAC 2 cut(s) 619, 1039
DdeI CTNAG 7 cut(s) 11, 735, 843, 852, 873, 891, 1433
DpnI GATC 6 cut(s) 205, 708, 714, 1094, 1286, 1389
DpnII GATC 6 cut(s) 203, 706, 712, 1092, 1284, 1387
DraI TTTAAA 2 cut(s) 952, 1161
DraIII CACNNNGTG 2 cut(s) 260, 518
EaeI YGGCCR 1 cut(s) 354
Eco32I GATATC 1 cut(s) 659
Eco57I CTGAAG 3 cut(s) 14, 1257, 1409
Eco88I CYCGRG 1 cut(s) 264
Eco91I GGTNACC 1 cut(s) 1367
EcoO65I GGTNACC 1 cut(s) 1367
EcoRII CCWGG 1 cut(s) 605
EcoRV GATATC 1 cut(s) 659
Esp3I CGTCTC 1 cut(s) 389
FaeI CATG 9 cut(s) 241, 379, 588, 829, 974, 1158, 1174, 1198, 1453
FalI AAGNNNNNCTT 2 cut(s) 105, 137
FaqI GGGAC 1 cut(s) 773
FatI CATG 9 cut(s) 237, 375, 584, 825, 970, 1154, 1170, 1194, 1449
FauI CCCGC 1 cut(s) 1463
Fnu4HI GCNGC 7 cut(s) 45, 312, 315, 354, 522, 810, 1240
FokI GGATG 1 cut(s) 655
Fsp4HI GCNGC 7 cut(s) 45, 312, 315, 354, 522, 810, 1240
FspBI CTAG 1 cut(s) 350
GluI GCNGC 7 cut(s) 45, 312, 315, 354, 522, 810, 1240
GsaI CCCAGC 1 cut(s) 918
GsuI CTGGAG 1 cut(s) 927
HaeIII GGCC 1 cut(s) 356
HapII CCGG 2 cut(s) 1070, 1365
HgaI GACGC 1 cut(s) 406
Hin1I GRCGYC 1 cut(s) 398
Hin1II CATG 9 cut(s) 241, 379, 588, 829, 974, 1158, 1174, 1198, 1453
HincII GTYRAC 4 cut(s) 154, 271, 373, 1058
HindII GTYRAC 4 cut(s) 154, 271, 373, 1058
HindIII AAGCTT 1 cut(s) 990
HpaI GTTAAC 2 cut(s) 271, 373
HpaII CCGG 2 cut(s) 1070, 1365
HphI GGTGA 4 cut(s) 72, 522, 987, 1361
Hpy166II GTNNAC 7 cut(s) 154, 271, 304, 373, 800, 1058, 1250
Hpy188I TCNGA 3 cut(s) 569, 626, 1097
Hpy188III TCNNGA 9 cut(s) 122, 238, 431, 710, 725, 872, 944, 1076, 1275
Hpy8I GTNNAC 7 cut(s) 154, 271, 304, 373, 800, 1058, 1250
HpyAV CCTTC 5 cut(s) 164, 682, 994, 1160, 1433
HpyCH4III ACNGT 5 cut(s) 181, 260, 385, 510, 1356
HpyCH4IV ACGT 2 cut(s) 1054, 1336
HpyF10VI GCNNNNNNNGC 3 cut(s) 31, 260, 1409
HpyF3I CTNAG 7 cut(s) 11, 735, 843, 852, 873, 891, 1433
HpySE526I ACGT 2 cut(s) 1054, 1336
Hsp92I GRCGYC 1 cut(s) 398
Hsp92II CATG 9 cut(s) 241, 379, 588, 829, 974, 1158, 1174, 1198, 1453
KspAI GTTAAC 2 cut(s) 271, 373
Kzo9I GATC 6 cut(s) 203, 706, 712, 1092, 1284, 1387
LmnI GCTCC 2 cut(s) 442, 806
Lsp1109I GCAGC 6 cut(s) 31, 298, 301, 533, 796, 1251
LweI GCATC 2 cut(s) 1043, 1312
MaeI CTAG 1 cut(s) 350
MaeII ACGT 2 cut(s) 1054, 1336
MaeIII GTNAC 3 cut(s) 510, 601, 1367
MalI GATC 6 cut(s) 205, 708, 714, 1094, 1286, 1389
MboI GATC 6 cut(s) 203, 706, 712, 1092, 1284, 1387
MflI RGATCY 2 cut(s) 706, 1284
MhlI GDGCHC 1 cut(s) 265
MluCI AATT 4 cut(s) 325, 465, 953, 1406
MlyI GAGTC 3 cut(s) 762, 893, 968
MmeI TCCRAC 1 cut(s) 605
MroXI GAANNNNTTC 1 cut(s) 117
MseI TTAA 6 cut(s) 270, 372, 951, 1028, 1160, 1382
MslI CAYNNNNRTG 1 cut(s) 1308
MspI CCGG 2 cut(s) 1070, 1365
MspR9I CCNGG 2 cut(s) 607, 1070
MvaI CCWGG 1 cut(s) 607
MvnI CGCG 1 cut(s) 1470
MwoI GCNNNNNNNGC 3 cut(s) 31, 260, 1409
NciI CCSGG 1 cut(s) 1070
NdeII GATC 6 cut(s) 203, 706, 712, 1092, 1284, 1387
NlaIII CATG 9 cut(s) 241, 379, 588, 829, 974, 1158, 1174, 1198, 1453
NmuCI GTSAC 2 cut(s) 510, 1367
NspI RCATGY 2 cut(s) 1158, 1174
PaeR7I CTCGAG 1 cut(s) 264
PagI TCATGA 1 cut(s) 237
PciI ACATGT 1 cut(s) 1154
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PdmI GAANNNNTTC 1 cut(s) 117
PfeI GAWTC 7 cut(s) 59, 234, 343, 475, 572, 1198, 1361
PflFI GACNNNGTC 2 cut(s) 749, 771
PkrI GCNGC 7 cut(s) 46, 313, 316, 355, 523, 811, 1241
PleI GAGTC 3 cut(s) 762, 893, 968
PpsI GAGTC 3 cut(s) 762, 893, 968
PscI ACATGT 1 cut(s) 1154
PsiI TTATAA 1 cut(s) 134
Psp1406I AACGTT 1 cut(s) 1336
Psp6I CCWGG 1 cut(s) 605
PspEI GGTNACC 1 cut(s) 1367
PspFI CCCAGC 1 cut(s) 914
PspGI CCWGG 1 cut(s) 605
PspXI VCTCGAGB 1 cut(s) 264
PstI CTGCAG 2 cut(s) 49, 301
PsuI RGATCY 2 cut(s) 706, 1284
PsyI GACNNNGTC 2 cut(s) 749, 771
RsaI GTAC 2 cut(s) 620, 1040
RsaNI GTAC 2 cut(s) 619, 1039
RseI CAYNNNNRTG 1 cut(s) 1308
SaqAI TTAA 6 cut(s) 270, 372, 951, 1028, 1160, 1382
SatI GCNGC 7 cut(s) 45, 312, 315, 354, 522, 810, 1240
Sau3AI GATC 6 cut(s) 203, 706, 712, 1092, 1284, 1387
SchI GAGTC 3 cut(s) 762, 893, 968
ScrFI CCNGG 2 cut(s) 607, 1070
SduI GDGCHC 1 cut(s) 265
SfaNI GCATC 2 cut(s) 1043, 1312
SfcI CTRYAG 2 cut(s) 45, 297
Sfr274I CTCGAG 1 cut(s) 264
SlaI CTCGAG 1 cut(s) 264
SmiMI CAYNNNNRTG 1 cut(s) 1308
SmlI CTYRAG 5 cut(s) 122, 264, 338, 1076, 1289
SmoI CTYRAG 5 cut(s) 122, 264, 338, 1076, 1289
Sse9I AATT 4 cut(s) 325, 465, 953, 1406
SsiI CCGC 2 cut(s) 353, 1470
SspMI CTAG 1 cut(s) 350
StyD4I CCNGG 2 cut(s) 605, 1068
TaaI ACNGT 5 cut(s) 181, 260, 385, 510, 1356
TaiI ACGT 2 cut(s) 1057, 1339
TaqI TCGA 6 cut(s) 147, 265, 454, 711, 744, 1201
TasI AATT 4 cut(s) 325, 465, 953, 1406
TauI GCSGC 1 cut(s) 356
TfiI GAWTC 7 cut(s) 59, 234, 343, 475, 572, 1198, 1361
Tru1I TTAA 6 cut(s) 270, 372, 951, 1028, 1160, 1382
Tru9I TTAA 6 cut(s) 270, 372, 951, 1028, 1160, 1382
TscAI CASTG 1 cut(s) 824
TseFI GTSAC 2 cut(s) 510, 1367
TseI GCWGC 6 cut(s) 44, 311, 314, 521, 809, 1239
Tsp45I GTSAC 2 cut(s) 510, 1367
TspDTI ATGAA 7 cut(s) 126, 254, 392, 467, 794, 946, 1466
TspGWI ACGGA 2 cut(s) 485, 1112
TspRI CASTG 1 cut(s) 824
Tth111I GACNNNGTC 2 cut(s) 749, 771
XapI RAATTY 1 cut(s) 953
XceI RCATGY 2 cut(s) 1158, 1174
XcmI CCANNNNNNNNNTGG 1 cut(s) 364
XhoI CTCGAG 1 cut(s) 264
XmnI GAANNNNTTC 1 cut(s) 117
XspI CTAG 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.