Rroxscaffold_2G00082770

OST-HTH/LOTUS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000679
Physical Location & Seq
Forward (+)
5533585 .. 5539237
5653 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00082770.1

Sequence Viewer

Length: 2865 bp
ATGAATCTCAGGAAATACGAAGCTTCGGAGCGTCGGAGCTCTTCAACTCCTCTCCGAAATGTAGCTACTTTTCTTATAACGGTGAAAGCGCCGGTTCGTGTTCCGTCGACGAAGCGGCTCGCCGGCGGCGACGTGCTGGACCGAGCGACGGCGTCGAAGCTCCTGCACCTCTGCTGCAACTTCGACTCGGCGGAGTGCGCCTCCGCGCTTCTCGTCGGCGAACTCGGGACGGTTCCGCTCGTGAACGAGTTCGACGACTCGGGGAAATCGCCTCTGCACACGGCGGCGTCGGCTCACGCGTCGCGGTGCGTGGAGGTTTTGCTGAAGAAACACGCTCGTACGGACCTGAGGACCAAGGACGGTCAGGCACAGCTAGCACTGGAATTGTCGCTTAGTGACACAAGGATGGACCTGAATTGGAATCCGAACGATTTCTCCATCGAAGACTTGGTGATTCACATGAGTGAAAAGGATTTGAGCACGGTGAAACTTCTGAGTGAAAAAACCAAGGAGATTGCTGACGTGGCTTATGGCTACGCCGTTAAAGGCCGTGTAGCTTCTCTGACGGCGCTGCTCATCGTCGCCGCCGAGAAGGTTAACGATTCCGTCTTGGAGTTGCGTGATTCCGAATCGGGGACCAAGGAGAAGGTGACTATCTACGAATGGCTTATAAGGGAGGCTTTGTCCCTGGGCGGCCGTACAGCGTTGGCGTTGAGGGCGGCCAAACGCGCCTCCACGGCTACGGAGGGCGAAAACTCGGAGAGGAGGAAGCTCGTGCTTCGAGAGATAGAACTGCTTCAGCTCTTCGGAGCTGTAGCTCAGAGCAGCGGTGGTGGGGACAAGAAGGTGACTTCGCCACTCATCCGTGCAGCTCAGGTCCTTCTGTTGGAAGACCCAGTTGGTGTCCAATATAGGACGGAAATGAAAGAAACCCCCCTGTTTTTCGCTGTGAAGAATGAGAGTAAGGAGTGTGCTGAGCTTCTTTTGACGTGGGGAGCAAATAGTGAAGTCCTTAATCTGCGTAGGCAAAGAGCTATAGACCTGGCAACCTCTCAAGATATGCGTTACATATTGCTGAACCCAACCACTGTTAGCCTCATTAATCATGCTTTCCCCAATCAACATAAATGTACGGCTTTAGAGTTGACAGATGAAGACACCACTAACGAAAGAAATGTTAACTCAAGCACCAAGGCAGAGGTTTGTAAGTACTTTGACTCGCATCGAGGATGTGTCAGAGGAGCTAAGTGCTTTTATGCACATTGTGAGGAGGAGTCTCGGAAAGTGAAGGAGGGAGCAGACCGGAGTCATTTTTCTGCTGCTAAACTACTCAAACGCAAAATCTTTGTTGGTGGACTTCCTCCTTCAGTGGACTCTGCAGATGATTTGGGAAAGTTCTGTGAAGAGGAGTTTGGATCAGTGGACGATGCTATAGTCATTGTCACTCAAATAGAAAACAAAATACAATCTCGAGGCTTTGGTTTTGTCACATTTAGAGAGGAGAAATCTGCTTCAGAAGCTATGCAAGCGCACTACATCACCATGAGAGGCAAGAAAGTTGAAATAAAAAGTTTAATTCCAAAATTGGTTTTGGGAGCTGAGTTTGAGAAGATGTCACCTCAACAACGACAACAAGATAAGAATATTCAAAATCAGCTTCCACAGCAGAAGTCGAACGAGAATATTATGGAAGGTGATAACCCTGAACAAGGTTCTTGGGCTGATAGATTAGTCCTTGGACAACCAGTGGCGTGTTCCACTGAACCTCAAGTTACCAAGAGCTCTGAGGATCCAAGCACTCCTACATGGCTAAAGGTTTTCAAGAAGTGGTTTCCTGGGTTCTTACAAGGTCTGTCAAAGCATTCAAATTATGCTCTGTCTTCTCTAAAAGCTGACTTCAGGGCTAAATTTGGGTTAGAACTGGATCACTCATCTGTGGGCTACTCTAAGCTCAGTGACTTTGTGAAATGTTTCTCCGATTTATGTACTGTTGAGATTCTCCCCATCTGCAAACGTGGAACTCCAACCCACATGATCCTCCGGCCAAAATTTTCTAGGCCTCAACGCCGACTCCTTCCTACACTGAGAACACCTCACACTGTGTCTCTGTCAGCACTGCTTGTCAAAGGCGGTGATACAAAGTGTCTTTTGGACCTCTCAAGTGGCGATTGTGGTGATTCAAAGTGTCTTCAGGACCATTCGAAGTGTTTTCTGGACCTTTCAGTTGACAATGGCAGTGACTCAAAGTGTTTTCAGAACCTTACAATTGAAAGCGACCTTTCACTTGACTTTGGTGGTGATTCAAAGTGTATTGAGGACCTTTCCATTTGCAATGCTGGTGAGAAGTTTGGCTCAAGTGTCACTAGCCATGGGCAAGAGAAACCATCGTCTGGGTATCCTGAAATGAACTCTGCAGAGGATAAAGTACAATGTGTGCACCCGAGGTTCCTTCAATTCTTAAAACCAGATCCTCTATTCTATGGTCAGAAAGAAACTGACGTGCTTGCCGGAGAGACTGACAATGAAAAGGGACAGTGCAGTGCAGGGGATAAAGGAAGTATAAATATTAGGGATCCAAGACGGCATCTCGTTTTGGAGTCCCTGTCCAAGAAATGGAGCAAATCCTACTTCCTTCGTGAATTTGATTTCTACAAGATTTACAAGGAAAGATTATTGGTAAGAAGGTGTTTTGCATGCAACCAGCAGAAGATGCTATGGGCCAATTTCCCTTGTAAACACTTGCTGTGGTGCACTGACTGTAAGCTCCTGGCAATAGAGGCTTCTGGTCTTTTCCCACACAAATGCGTGCTGTGTGACACAGAAGTACAGAAAATGGATTTAGTACTTCCATTAACTAGTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 OST-HTH RRM_1

Protein Analysis

954

Amino Acids

106.11

Weight (kDa)

7.64

Isoelectric Point (pI)

44.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 50 - 115 1.6e-06 Ankyrin repeats (3 copies)
RRM_1 PF00076 448 - 520 3.3e-09 RNA recognition motif
OST-HTH PF12872 603 - 665 6.9e-08 OST-HTH/LOTUS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 77, 671
AccB7I CCANNNNNTGG 2 cut(s) 2390, 2613
AccBSI CCGCTC 1 cut(s) 238
AccI GTMKAC 1 cut(s) 107
AccII CGCG 4 cut(s) 206, 299, 304, 729
AclWI GGATC 8 cut(s) 1423, 1784, 1797, 1932, 2029, 2462, 2567, 2580
AcoI YGGCCR 3 cut(s) 694, 720, 2042
AcsI RAATTY 3 cut(s) 1907, 2048, 2639
AcuI CTGAAG 6 cut(s) 344, 782, 1350, 1497, 1882, 2174
AcyI GRCGYC 2 cut(s) 152, 287
AdeI CACNNNGTG 2 cut(s) 1265, 2101
AfaI GTAC 8 cut(s) 340, 700, 1132, 1211, 1987, 2427, 2826, 2844
AfiI CCNNNNNNNGG 6 cut(s) 148, 633, 886, 1709, 2390, 2613
AflIII ACRYGT 1 cut(s) 297
AgsI TTSAA 9 cut(s) 45, 1562, 1649, 1822, 1866, 2181, 2270, 2304, 2453
AhlI ACTAGT 1 cut(s) 2855
AjiI CACGTC 4 cut(s) 133, 523, 990, 2500
AjnI CCWGG 4 cut(s) 687, 1041, 1834, 2766
AjuI GAANNNNNNNTTGG 4 cut(s) 882, 914, 1395, 1427
AleI CACNNNNGTG 1 cut(s) 462
AloI GAACNNNNNNTCC 2 cut(s) 419, 451
Alw21I GWGCWC 5 cut(s) 41, 482, 1784, 2439, 2753
Alw26I GTCTC 3 cut(s) 1281, 2109, 2507
Alw44I GTGCAC 2 cut(s) 2435, 2749
AlwI GGATC 8 cut(s) 1423, 1784, 1797, 1932, 2029, 2462, 2567, 2580
Ama87I CYCGRG 4 cut(s) 224, 259, 1470, 2440
AoxI GGCC 6 cut(s) 547, 694, 720, 2042, 2057, 2718
ApaLI GTGCAC 2 cut(s) 2435, 2749
ApeKI GCWGC 5 cut(s) 174, 571, 825, 869, 1319
ApoI RAATTY 3 cut(s) 1907, 2048, 2639
AseI ATTAAT 1 cut(s) 1101
Asp700I GAANNNNTTC 4 cut(s) 248, 431, 795, 1970
AspLEI GCGC 6 cut(s) 91, 200, 208, 571, 731, 1531
AsuII TTCGAA 1 cut(s) 2201
AsuNHI GCTAGC 1 cut(s) 373
AvaI CYCGRG 4 cut(s) 224, 259, 1470, 2440
AxyI CCTNAGG 1 cut(s) 347
BaeGI GKGCMC 2 cut(s) 2439, 2753
BamHI GGATCC 2 cut(s) 1789, 2572
BanII GRGCYC 2 cut(s) 41, 1784
BauI CACGAG 2 cut(s) 239, 773
BbsI GAAGAC 5 cut(s) 450, 897, 1161, 1872, 2180
Bbv12I GWGCWC 5 cut(s) 41, 482, 1784, 2439, 2753
BbvI GCAGC 5 cut(s) 161, 558, 837, 881, 1306
BccI CCATC 4 cut(s) 400, 446, 2012, 2392
BceAI ACGGC 9 cut(s) 165, 297, 524, 534, 582, 681, 753, 1149, 2597
BcgI CGANNNNNNTGC 2 cut(s) 145, 179
BciT130I CCWGG 4 cut(s) 689, 1043, 1836, 2768
BciVI GTATCC 1 cut(s) 2406
BcoDI GTCTC 3 cut(s) 1281, 2109, 2507
BcuI ACTAGT 1 cut(s) 2855
BfaI CTAG 4 cut(s) 374, 2055, 2364, 2856
BfmI CTRYAG 5 cut(s) 813, 1035, 1377, 1431, 2412
BfoI RGCGCY 2 cut(s) 92, 572
BfuI GTATCC 1 cut(s) 2406
BglI GCCNNNNNGGC 1 cut(s) 737
BlpI GCTNAGC 1 cut(s) 975
BmcAI AGTACT 2 cut(s) 1211, 2844
Bme1390I CCNGG 4 cut(s) 689, 1043, 1836, 2768
BmeT110I CYCGRG 4 cut(s) 224, 259, 1470, 2440
BmgBI CACGTC 4 cut(s) 133, 523, 990, 2500
BmiI GGNNCC 5 cut(s) 234, 637, 1791, 2447, 2574
BmrFI CCNGG 4 cut(s) 689, 1043, 1836, 2768
BmrI ACTGGG 1 cut(s) 890
BmsI GCATC 4 cut(s) 1231, 1417, 2593, 2700
BmtI GCTAGC 1 cut(s) 377
BmuI ACTGGG 1 cut(s) 890
BoxI GACNNNNGTC 1 cut(s) 1305
BpiI GAAGAC 5 cut(s) 450, 897, 1161, 1872, 2180
BplI GAGNNNNNCTC 4 cut(s) 185, 217, 2077, 2109
Bpu10I CCTNAGC 1 cut(s) 873
Bpu1102I GCTNAGC 1 cut(s) 975
Bpu14I TTCGAA 1 cut(s) 2201
BpuEI CTTGAG 5 cut(s) 1038, 1168, 1752, 2143, 2338
BsaHI GRCGYC 2 cut(s) 152, 287
BsaWI WCCGGW 1 cut(s) 1302
BsaXI ACNNNNNCTCC 8 cut(s) 419, 449, 668, 698, 2055, 2085, 2588, 2618
Bsc4I CCNNNNNNNGG 6 cut(s) 148, 633, 886, 1709, 2390, 2613
Bse118I RCCGGY 2 cut(s) 91, 122
Bse1I ACTGG 4 cut(s) 384, 896, 1745, 1926
Bse21I CCTNAGG 1 cut(s) 347
Bse3DI GCAATG 1 cut(s) 2338
BseBI CCWGG 4 cut(s) 689, 1043, 1836, 2768
BseGI GGATG 3 cut(s) 411, 861, 1235
BseLI CCNNNNNNNGG 6 cut(s) 148, 633, 886, 1709, 2390, 2613
BseMI GCAATG 1 cut(s) 2338
BseNI ACTGG 4 cut(s) 384, 896, 1745, 1926
BseRI GAGGAG 7 cut(s) 39, 778, 1254, 1283, 1286, 1421, 1514
BseSI GKGCMC 2 cut(s) 2439, 2753
BseX3I CGGCCG 1 cut(s) 694
BseXI GCAGC 5 cut(s) 161, 558, 837, 881, 1306
BsgI GTGCAG 5 cut(s) 149, 260, 888, 2557, 2562
Bsh1236I CGCG 4 cut(s) 206, 299, 304, 729
Bsh1285I CGRYCG 1 cut(s) 697
BshFI GGCC 6 cut(s) 549, 696, 722, 2044, 2059, 2720
BsiEI CGRYCG 1 cut(s) 697
BsiHKAI GWGCWC 5 cut(s) 41, 482, 1784, 2439, 2753
BsiHKCI CYCGRG 4 cut(s) 224, 259, 1470, 2440
BsiSI CCGG 5 cut(s) 92, 123, 1303, 2041, 2508
BsiWI CGTACG 1 cut(s) 338
BslFI GGGAC 6 cut(s) 241, 649, 670, 851, 2544, 2584
BslI CCNNNNNNNGG 6 cut(s) 148, 633, 886, 1709, 2390, 2613
BsmAI GTCTC 3 cut(s) 1281, 2109, 2507
BsmFI GGGAC 6 cut(s) 241, 649, 670, 851, 2544, 2584
BsmI GAATGC 1 cut(s) 1861
BsnI GGCC 6 cut(s) 549, 696, 722, 2044, 2059, 2720
BsoBI CYCGRG 4 cut(s) 224, 259, 1470, 2440
Bsp119I TTCGAA 1 cut(s) 2201
Bsp1286I GDGCHC 5 cut(s) 41, 482, 1784, 2439, 2753
Bsp143I GATC 6 cut(s) 1415, 1789, 1924, 2034, 2467, 2572
Bsp1720I GCTNAGC 1 cut(s) 975
Bsp19I CCATGG 1 cut(s) 2368
BspANI GGCC 6 cut(s) 549, 696, 722, 2044, 2059, 2720
BspFNI CGCG 4 cut(s) 206, 299, 304, 729
BspLI GGNNCC 5 cut(s) 234, 637, 1791, 2447, 2574
BspMAI CTGCAG 2 cut(s) 1381, 2416
BspOI GCTAGC 1 cut(s) 377
BspPI GGATC 8 cut(s) 1423, 1784, 1797, 1932, 2029, 2462, 2567, 2580
BspQI GCTCTTC 2 cut(s) 46, 809
BspT104I TTCGAA 1 cut(s) 2201
BsrBI CCGCTC 1 cut(s) 238
BsrDI GCAATG 1 cut(s) 2338
BsrFI RCCGGY 2 cut(s) 91, 122
BsrI ACTGG 4 cut(s) 384, 896, 1745, 1926
BssAI RCCGGY 2 cut(s) 91, 122
BssMI GATC 6 cut(s) 1415, 1789, 1924, 2034, 2467, 2572
BssNI GRCGYC 2 cut(s) 152, 287
BssSI CACGAG 2 cut(s) 239, 773
BssT1I CCWWGG 6 cut(s) 354, 507, 639, 1191, 1735, 2368
Bst2BI CACGAG 2 cut(s) 239, 773
Bst2UI CCWGG 4 cut(s) 689, 1043, 1836, 2768
Bst4CI ACNGT 9 cut(s) 82, 232, 362, 484, 1090, 1990, 2101, 2535, 2759
Bst6I CTCTTC 3 cut(s) 46, 809, 1398
BstACI GRCGYC 2 cut(s) 152, 287
BstAPI GCANNNNNTGC 1 cut(s) 2710
BstBI TTCGAA 1 cut(s) 2201
BstC8I GCNNGC 7 cut(s) 120, 124, 375, 1527, 2505, 2695, 2807
BstDSI CCRYGG 2 cut(s) 735, 2368
BstENI CCTNNNNNAGG 1 cut(s) 1707
BstF5I GGATG 3 cut(s) 411, 861, 1235
BstFNI CGCG 4 cut(s) 206, 299, 304, 729
BstH2I RGCGCY 2 cut(s) 92, 572
BstHHI GCGC 6 cut(s) 91, 200, 208, 571, 731, 1531
BstKTI GATC 6 cut(s) 1418, 1792, 1927, 2037, 2470, 2575
BstMAI GTCTC 3 cut(s) 1281, 2109, 2507
BstMBI GATC 6 cut(s) 1415, 1789, 1924, 2034, 2467, 2572
BstMCI CGRYCG 1 cut(s) 697
BstNI CCWGG 4 cut(s) 689, 1043, 1836, 2768
BstNSI RCATGY 1 cut(s) 2697
BstPAI GACNNNNGTC 1 cut(s) 1305
BstSCI CCNGG 4 cut(s) 687, 1041, 1834, 2766
BstSFI CTRYAG 5 cut(s) 813, 1035, 1377, 1431, 2412
BstSLI GKGCMC 2 cut(s) 2439, 2753
BstUI CGCG 4 cut(s) 206, 299, 304, 729
BstV1I GCAGC 5 cut(s) 161, 558, 837, 881, 1306
BstV2I GAAGAC 5 cut(s) 450, 897, 1161, 1872, 2180
BstX2I RGATCY 3 cut(s) 1789, 2467, 2572
BstYI RGATCY 3 cut(s) 1789, 2467, 2572
BstZI CGGCCG 1 cut(s) 694
Bsu36I CCTNAGG 1 cut(s) 347
BsuI GTATCC 1 cut(s) 2406
BsuRI GGCC 6 cut(s) 549, 696, 722, 2044, 2059, 2720
BtgI CCRYGG 2 cut(s) 735, 2368
BtrI CACGTC 4 cut(s) 133, 523, 990, 2500
BtsCI GGATG 3 cut(s) 411, 861, 1235
BtsI GCAGTG 3 cut(s) 2114, 2242, 2545
Cac8I GCNNGC 7 cut(s) 120, 124, 375, 1527, 2505, 2695, 2807
CfoI GCGC 6 cut(s) 91, 200, 208, 571, 731, 1531
Cfr10I RCCGGY 2 cut(s) 91, 122
CseI GACGC 4 cut(s) 20, 141, 276, 288
Csp6I GTAC 8 cut(s) 339, 699, 1131, 1210, 1986, 2426, 2825, 2843
CviAII CATG 7 cut(s) 460, 1106, 1543, 1806, 2032, 2369, 2694
CviQI GTAC 8 cut(s) 339, 699, 1131, 1210, 1986, 2426, 2825, 2843
DpnI GATC 6 cut(s) 1417, 1791, 1926, 2036, 2469, 2574
DpnII GATC 6 cut(s) 1415, 1789, 1924, 2034, 2467, 2572
DraIII CACNNNGTG 2 cut(s) 1265, 2101
EaeI YGGCCR 3 cut(s) 694, 720, 2042
EagI CGGCCG 1 cut(s) 694
Eam1104I CTCTTC 3 cut(s) 46, 809, 1398
EarI CTCTTC 3 cut(s) 46, 809, 1398
EciI GGCGGA 1 cut(s) 206
Ecl136II GAGCTC 2 cut(s) 39, 1782
EclXI CGGCCG 1 cut(s) 694
Eco130I CCWWGG 6 cut(s) 354, 507, 639, 1191, 1735, 2368
Eco147I AGGCCT 1 cut(s) 2059
Eco24I GRGCYC 2 cut(s) 41, 1784
Eco52I CGGCCG 1 cut(s) 694
Eco53kI GAGCTC 2 cut(s) 39, 1782
Eco57I CTGAAG 6 cut(s) 344, 782, 1350, 1497, 1882, 2174
Eco81I CCTNAGG 1 cut(s) 347
Eco88I CYCGRG 4 cut(s) 224, 259, 1470, 2440
EcoICRI GAGCTC 2 cut(s) 39, 1782
EcoNI CCTNNNNNAGG 1 cut(s) 1707
EcoO109I RGGNCCY 2 cut(s) 877, 2317
EcoRII CCWGG 4 cut(s) 687, 1041, 1834, 2766
EcoT14I CCWWGG 6 cut(s) 354, 507, 639, 1191, 1735, 2368
EcoT38I GRGCYC 2 cut(s) 41, 1784
ErhI CCWWGG 6 cut(s) 354, 507, 639, 1191, 1735, 2368
FaeI CATG 7 cut(s) 463, 1109, 1546, 1809, 2035, 2372, 2697
FalI AAGNNNNNCTT 2 cut(s) 664, 696
FaqI GGGAC 6 cut(s) 241, 649, 670, 851, 2544, 2584
FatI CATG 7 cut(s) 459, 1105, 1542, 1805, 2031, 2368, 2693
FblI GTMKAC 1 cut(s) 107
FokI GGATG 3 cut(s) 418, 848, 1242
FriOI GRGCYC 2 cut(s) 41, 1784
FspBI CTAG 4 cut(s) 374, 2055, 2364, 2856
GlaI GCGC 6 cut(s) 90, 199, 207, 570, 730, 1530
HaeII RGCGCY 2 cut(s) 92, 572
HaeIII GGCC 6 cut(s) 549, 696, 722, 2044, 2059, 2720
HapII CCGG 5 cut(s) 92, 123, 1303, 2041, 2508
HgaI GACGC 4 cut(s) 20, 141, 276, 288
HhaI GCGC 6 cut(s) 91, 200, 208, 571, 731, 1531
Hin1I GRCGYC 2 cut(s) 152, 287
Hin1II CATG 7 cut(s) 463, 1109, 1546, 1809, 2035, 2372, 2697
Hin6I GCGC 6 cut(s) 89, 198, 206, 569, 729, 1529
HinP1I GCGC 6 cut(s) 89, 198, 206, 569, 729, 1529
HincII GTYRAC 5 cut(s) 108, 598, 1146, 1180, 2227
HindII GTYRAC 5 cut(s) 108, 598, 1146, 1180, 2227
HindIII AAGCTT 1 cut(s) 21
HpaI GTTAAC 2 cut(s) 598, 1180
HpaII CCGG 5 cut(s) 92, 123, 1303, 2041, 2508
HpyCH4III ACNGT 9 cut(s) 82, 232, 362, 484, 1090, 1990, 2101, 2535, 2759
HpyCH4IV ACGT 5 cut(s) 132, 522, 989, 2014, 2499
HpySE526I ACGT 5 cut(s) 132, 522, 989, 2014, 2499
Hsp92I GRCGYC 2 cut(s) 152, 287
Hsp92II CATG 7 cut(s) 463, 1109, 1546, 1809, 2035, 2372, 2697
HspAI GCGC 6 cut(s) 89, 198, 206, 569, 729, 1529
KroI GCCGGC 1 cut(s) 122
KroNI GCCGGC 1 cut(s) 124
KspAI GTTAAC 2 cut(s) 598, 1180
Kzo9I GATC 6 cut(s) 1415, 1789, 1924, 2034, 2467, 2572
LguI GCTCTTC 2 cut(s) 46, 809
Lsp1109I GCAGC 5 cut(s) 161, 558, 837, 881, 1306
LweI GCATC 4 cut(s) 1231, 1417, 2593, 2700
MaeI CTAG 4 cut(s) 374, 2055, 2364, 2856
MaeII ACGT 5 cut(s) 132, 522, 989, 2014, 2499
MalI GATC 6 cut(s) 1417, 1791, 1926, 2036, 2469, 2574
MbiI CCGCTC 1 cut(s) 238
MboI GATC 6 cut(s) 1415, 1789, 1924, 2034, 2467, 2572
MfeI CAATTG 1 cut(s) 2265
MflI RGATCY 3 cut(s) 1789, 2467, 2572
MhlI GDGCHC 5 cut(s) 41, 482, 1784, 2439, 2753
MluI ACGCGT 1 cut(s) 297
MlyI GAGTC 9 cut(s) 179, 251, 1211, 1283, 1315, 1367, 2064, 2234, 2606
MmeI TCCRAC 3 cut(s) 14, 867, 2048
MreI CGCCGGCG 1 cut(s) 122
MroNI GCCGGC 1 cut(s) 122
MroXI GAANNNNTTC 4 cut(s) 248, 431, 795, 1970
MseI TTAA 8 cut(s) 543, 597, 1014, 1101, 1179, 1574, 2459, 2852
MslI CAYNNNNRTG 4 cut(s) 404, 462, 1541, 2800
MspA1I CMGCKG 1 cut(s) 828
MspI CCGG 5 cut(s) 92, 123, 1303, 2041, 2508
MspR9I CCNGG 4 cut(s) 689, 1043, 1836, 2768
MunI CAATTG 1 cut(s) 2265
Mva1269I GAATGC 1 cut(s) 1861
MvaI CCWGG 4 cut(s) 689, 1043, 1836, 2768
MvnI CGCG 4 cut(s) 206, 299, 304, 729
NaeI GCCGGC 1 cut(s) 124
NcoI CCATGG 1 cut(s) 2368
NdeII GATC 6 cut(s) 1415, 1789, 1924, 2034, 2467, 2572
NgoMIV GCCGGC 1 cut(s) 122
NheI GCTAGC 1 cut(s) 373
NlaIII CATG 7 cut(s) 463, 1109, 1546, 1809, 2035, 2372, 2697
NlaIV GGNNCC 5 cut(s) 234, 637, 1791, 2447, 2574
NmeAIII GCCGAG 2 cut(s) 167, 613
NspI RCATGY 1 cut(s) 2697
NspV TTCGAA 1 cut(s) 2201
OliI CACNNNNGTG 1 cut(s) 462
PaeI GCATGC 1 cut(s) 2697
PaeR7I CTCGAG 1 cut(s) 1470
PasI CCCWGGG 1 cut(s) 688
PceI AGGCCT 1 cut(s) 2059
PciSI GCTCTTC 2 cut(s) 46, 809
PcsI WCGNNNNNNNCGW 4 cut(s) 126, 252, 296, 585
PctI GAATGC 1 cut(s) 1861
PdiI GCCGGC 1 cut(s) 124
PdmI GAANNNNTTC 4 cut(s) 248, 431, 795, 1970
PfeI GAWTC 9 cut(s) 4, 421, 454, 602, 623, 629, 1996, 2177, 2300
Pfl23II CGTACG 1 cut(s) 338
PflFI GACNNNGTC 1 cut(s) 151
PflMI CCANNNNNTGG 2 cut(s) 2390, 2613
PleI GAGTC 9 cut(s) 179, 251, 1211, 1282, 1314, 1367, 2064, 2234, 2605
PpsI GAGTC 9 cut(s) 179, 251, 1211, 1282, 1314, 1367, 2064, 2234, 2605
PpuMI RGGWCCY 2 cut(s) 877, 2317
PshAI GACNNNNGTC 1 cut(s) 1305
PshBI ATTAAT 1 cut(s) 1101
PsiI TTATAA 2 cut(s) 77, 671
Psp124BI GAGCTC 2 cut(s) 41, 1784
Psp5II RGGWCCY 2 cut(s) 877, 2317
Psp6I CCWGG 4 cut(s) 687, 1041, 1834, 2766
PspGI CCWGG 4 cut(s) 687, 1041, 1834, 2766
PspLI CGTACG 1 cut(s) 338
PspN4I GGNNCC 5 cut(s) 234, 637, 1791, 2447, 2574
PspPPI RGGWCCY 2 cut(s) 877, 2317
PstI CTGCAG 2 cut(s) 1381, 2416
PsuI RGATCY 3 cut(s) 1789, 2467, 2572
PsyI GACNNNGTC 1 cut(s) 151
RsaI GTAC 8 cut(s) 340, 700, 1132, 1211, 1987, 2427, 2826, 2844
RsaNI GTAC 8 cut(s) 339, 699, 1131, 1210, 1986, 2426, 2825, 2843
RseI CAYNNNNRTG 4 cut(s) 404, 462, 1541, 2800
SacI GAGCTC 2 cut(s) 41, 1784
SalI GTCGAC 1 cut(s) 106
SapI GCTCTTC 2 cut(s) 46, 809
SaqAI TTAA 8 cut(s) 543, 597, 1014, 1101, 1179, 1574, 2459, 2852
Sau3AI GATC 6 cut(s) 1415, 1789, 1924, 2034, 2467, 2572
ScaI AGTACT 2 cut(s) 1211, 2844
SchI GAGTC 9 cut(s) 179, 251, 1211, 1283, 1315, 1367, 2064, 2234, 2606
ScrFI CCNGG 4 cut(s) 689, 1043, 1836, 2768
SduI GDGCHC 5 cut(s) 41, 482, 1784, 2439, 2753
SfaNI GCATC 4 cut(s) 1231, 1417, 2593, 2700
SfcI CTRYAG 5 cut(s) 813, 1035, 1377, 1431, 2412
Sfr274I CTCGAG 1 cut(s) 1470
SfuI TTCGAA 1 cut(s) 2201
SgrAI CRCCGGYG 1 cut(s) 122
SgrDI CGTCGACG 1 cut(s) 106
SlaI CTCGAG 1 cut(s) 1470
SmiMI CAYNNNNRTG 4 cut(s) 404, 462, 1541, 2800
SmlI CTYRAG 6 cut(s) 1053, 1183, 1470, 1767, 2158, 2353
SmoI CTYRAG 6 cut(s) 1053, 1183, 1470, 1767, 2158, 2353
SpeI ACTAGT 1 cut(s) 2855
SphI GCATGC 1 cut(s) 2697
SseBI AGGCCT 1 cut(s) 2059
SspI AATATT 3 cut(s) 1645, 1684, 2566
SspMI CTAG 4 cut(s) 374, 2055, 2364, 2856
SstI GAGCTC 2 cut(s) 41, 1784
StuI AGGCCT 1 cut(s) 2059
StyD4I CCNGG 4 cut(s) 687, 1041, 1834, 2766
StyI CCWWGG 6 cut(s) 354, 507, 639, 1191, 1735, 2368
TaaI ACNGT 9 cut(s) 82, 232, 362, 484, 1090, 1990, 2101, 2535, 2759
TaiI ACGT 5 cut(s) 135, 525, 992, 2017, 2502
TaqII GACCGA 1 cut(s) 156
TatI WGTACW 5 cut(s) 1209, 1985, 2425, 2824, 2842
TauI GCSGC 6 cut(s) 118, 129, 287, 587, 696, 722
TfiI GAWTC 9 cut(s) 4, 421, 454, 602, 623, 629, 1996, 2177, 2300
Tru1I TTAA 8 cut(s) 543, 597, 1014, 1101, 1179, 1574, 2459, 2852
Tru9I TTAA 8 cut(s) 543, 597, 1014, 1101, 1179, 1574, 2459, 2852
TseI GCWGC 5 cut(s) 174, 571, 825, 869, 1319
TspDTI ATGAA 5 cut(s) 17, 938, 1167, 2420, 2538
TspGWI ACGGA 6 cut(s) 93, 356, 595, 758, 854, 932
Tth111I GACNNNGTC 1 cut(s) 151
Van91I CCANNNNNTGG 2 cut(s) 2390, 2613
VneI GTGCAC 2 cut(s) 2435, 2749
VspI ATTAAT 1 cut(s) 1101
XagI CCTNNNNNAGG 1 cut(s) 1707
XapI RAATTY 3 cut(s) 1907, 2048, 2639
XceI RCATGY 1 cut(s) 2697
XcmI CCANNNNNNNNNTGG 1 cut(s) 445
XhoI CTCGAG 1 cut(s) 1470
XmiI GTMKAC 1 cut(s) 107
XmnI GAANNNNTTC 4 cut(s) 248, 431, 795, 1970
XspI CTAG 4 cut(s) 374, 2055, 2364, 2856
ZrmI AGTACT 2 cut(s) 1211, 2844
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.