Rw2G050430

OST-HTH/LOTUS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000679
Physical Location & Seq
Forward (+)
78057083 .. 78066137
9055 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G050430.1

Sequence Viewer

Length: 3096 bp
ATGAATCGTAGGAAAAACGAAGCTTCGGAGGAGCCTCGGAGCCGTCTATCTCGACGACAAGGACGAGCTCGTGGCTCAGATGAAGCAGCTCGCCGGCGACTCACTGAACCTAGAGACGGCGTCGAAGCTCCTCCACCTCTGCTGCAAATGTACGTGTTGCTGAAGAAACACGCTCGTACGGACCTGAAGACCAGGGACGGTCAGGCACAGCTAGCACTGGAGCTGTCTCTTAGTGACGCAAGTCCCAACTTCAATTTGGTAATTCACCTAAGTGAAAAGGATTTGAGCACTGTGAAACTTTTAAGTGAAAAATCCAAAGAGATTGCTGAGAAGGTTAACGACTCCGCTTTGGAGTTGCGTGATTCCGACTCAGGTTGCAAGGAGAAGGTGACCATCTACGAATGCCTTATAAGGGAGGCTTTGGCCCTGGGCGGCCGTACAACGAAGCCGTCGAATTCTGAGAGAAGGAAACGGTTGCTTTATGTCATCCAGCTGCTTCTGAAGACAAATATAGATGTCAATTATGCTGATGCAGATGGAAATTCTGTTCTTCATGGGATACTTGGGTTGTCGAGATTATCATGTCCACTACAGATGAGAATTGTATTGCTTCTTCTCGAGCATGGCGCTCTAGTAAGCCAAAGGAATAACTTGGGGTTGACAGAATTTCATATTGCTGCCAGTAATGGTAATTCTGAGGCCCTTGAGGTCCTTCTGTTGGAAGACCCAGATGGAGTGCAGTATATGACGGAAATGAAAGAAACCACTCTGTTTTTCGCTGTGAAGAATGAAAGTGAGGACTGTGCTAAGCTTCTTTTGACTTGGGGAGCAAATAGTGAAGTCCTTAATCTACTTACGCAAAGAGCTATAGACCTGGCAACCTCTCAAGAGATGCGTTACATAGTGCTGAATCCAACCACTGTTAGCCTTATTGATCATGCCTTCCCCAATCAATTTGAATGTATGGCTCTGGAGTTGGCAGATGAAGACACCACTACCAAAAGTTCTCATGTTTCTACCCCTGTGAATGCTAGAAATGTTAACTCAAACACCAAGGCAGAGGTTTGTAAGTTCTTCAACCCTCATCAAGGATGTGTCAGGGGAGCAAAGTGCTTTAATGCACATTGTGAGGAGTGTCAGAAAGTGAAGGAGGAAGCAGACTGGAATCATTCTTCTGCTGCTAAACTAAAACGCAAAGTCTTTGTTGGAGGACTTCCTCTTTCAGTGGACTCTGATGCTTTGGGAAAGCTCTGTGAAAAAGAGTTTAGATCGGTGGAAGATGCTATAGTTATTGTAACTCGAATTGAAAACAAGATACAATCTCGAGGCTTTGGATTTGTCACATTTAGAGAGGAGAAATCTGCTTCGGAAGCCATACAAGCGCACTACATCACCACGAGGGCAAGAAACCCACACGTTGTGGTTAAGCGACAAGTTTTGTTCCTGGCGTCGTTCCCTTTCTGGAAAGCTATAGCAATCACGAATCGGACACCGAATGCCAAAGTTGCAATAATAACTTGGGTTTACCTCCTTTTGCACGATCGAACAGTTCTTCAATTCGGACTGCCATTCATTCTACATCAGGTTTCCTGGGTTCTTACAAGGTCTGTCAAAGCATTCAAATTATCTGTCTCTTCTCTAAAATGTGACTTCAGGACAAAATTTGGGTTAGAACTGGATCACTCATCTGTGGGCTACTCTAAGCTTAGTGACTTCCTGAAGTGTGTCTCCGATTTATGTGCCATTGAGATTCTCCCCATATGCAAACACGGAACTCCAACTCACATGACCCTCCGGCCAAAATTTTCTAGGCCTCAATGCCGACTCCTTCCTACACTCAGAACACCTTACACTCTGTCTTTGTCTACATTCATTATGAATGTGGGTGATTCAAAGTGTCTTTTAGACCTCTCAAGTGACGATGGTGGTGATTCAAAGGGTCTTCAGATCCTTTCAACTGGCAGCGGCACTGACAATGATGTTGATTCAAAGTGCCTTCAGGACCTTTCACTTGACTCTGGTAGTGATTCAAAGTGTATTGAAGACGAAATATGGAGCTCAAGTGTCACTAGCCATGTGGAAGAGAAACCCTCCTCTAGGTATCCTGAAATGAACTCTACACAAGATAAAGCACACTGTTTGCACCCAAGGTTCCTTCAATTCTTGAAGATAGATCCTCTACTCCATGGTCAGAATGATATTGATGTGCTTGCTGGAGAGACTGACAATGCAAAGGGACAGTGCAATGCAGGGGATGAGAATACAAGCGAATCATATTTCATTCATGACTCTGATTCTCGCAAGCATAATGTATATGTTAAAGGCACAACAAACCTTATGTGGGTTTTAAAAGAATGCGTACTCGTTGGTTGTGATTCACGATCCTCCACAGTGAATGGAGATATAGACTCAGATAGTGTCGAGAAATTCACACTGATAACCGACCATGTGCTTGCTACAAAGTGTGGTTACGATGTAGAAGCCACTCAAACATGGAAGCACATGGCGCTTTCCATCAGTAATGAGGTCAGATCTGGAATAGATCCAGATCATGTACGGAGAGTAGGCATGGCTGGTCGTTTGATGGAGAGAAGTGCCGATGCCAACTCCTCTAAATTCTTGATCGTTGGATTACAATATGAGATTGCAATTGGCTCTAGCTCGGCAACTGCCCGAAACATATTGGCACGAAGCTGGATTGAACATGGAAATGATCTGCCGTTGAAAGAGGGCATTCAAACTATAATCTGGTCATTGCTTGTAGCCGCTTTATCTGATCCTTTCACTGGTGGATTATTGACAGATGGATCACTGGACATGCGTACCACTGCTTTCCGTGATATCGAAGGAGTATCGGTATTTTTGTTCTATGTGCCGAACTATGTCAACAAAAAAGTTTTGGAGAATCGACTAAAAGTGATTTTCGAGACAGAAGCACATTCCACTAGATTTGTTGAAACAACTGAAGAGATTCCTGAGCATATACAGCTTCCACTCTCCACTTTGCGGGACTACTTTAGATTCATGAAGATCCAAAAGAGGAAAGTGCTTTTCAAGATAGATGCACATTCTACTAGATTTTTTGAAGCAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 OST-HTH RRM_1

Protein Analysis

1031

Amino Acids

115.26

Weight (kDa)

6.53

Isoelectric Point (pI)

40.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 201 - 278 1.9e-08 Ankyrin repeats (3 copies)
RRM_1 PF00076 400 - 461 1.3e-08 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 410
AccI GTMKAC 1 cut(s) 1865
AciI CCGC 5 cut(s) 345, 432, 1965, 2766, 3007
AclWI GGATC 8 cut(s) 1686, 1942, 2168, 2376, 2537, 2771, 2815, 3025
AcoI YGGCCR 2 cut(s) 433, 1796
AcsI RAATTY 7 cut(s) 454, 541, 665, 1661, 1802, 2426, 2615
AcuI CTGAAG 8 cut(s) 182, 206, 521, 1636, 1739, 1928, 1982, 2985
AcyI GRCGYC 2 cut(s) 120, 1448
AdeI CACNNNGTG 2 cut(s) 1127, 1420
AfaI GTAC 6 cut(s) 152, 178, 439, 2361, 2556, 2824
AfiI CCNNNNNNNGG 8 cut(s) 116, 412, 718, 1088, 2097, 2341, 2786, 3006
AflIII ACRYGT 2 cut(s) 153, 1414
AjnI CCWGG 5 cut(s) 191, 426, 873, 1443, 1589
AleI CACNNNNGTG 1 cut(s) 270
AloI GAACNNNNNNTCC 2 cut(s) 531, 563
Alw21I GWGCWC 3 cut(s) 70, 290, 2060
Alw26I GTCTC 6 cut(s) 108, 231, 1636, 1732, 2213, 2921
AlwI GGATC 8 cut(s) 1686, 1942, 2168, 2376, 2537, 2771, 2815, 3025
Ama87I CYCGRG 2 cut(s) 617, 1323
AoxI GGCC 5 cut(s) 423, 433, 699, 1796, 1811
ApeKI GCWGC 6 cut(s) 86, 142, 493, 677, 1178, 1962
ApoI RAATTY 7 cut(s) 454, 541, 665, 1661, 1802, 2426, 2615
ArsI GACNNNNNNTTYG 2 cut(s) 1614, 1646
Asp700I GAANNNNTTC 1 cut(s) 2970
AspLEI GCGC 3 cut(s) 629, 1384, 2509
AspS9I GGNCC 5 cut(s) 181, 424, 700, 709, 2002
AsuHPI GGTGA 5 cut(s) 257, 400, 1384, 1898, 1940
AsuNHI GCTAGC 1 cut(s) 211
AvaI CYCGRG 2 cut(s) 617, 1323
AvaII GGWCC 3 cut(s) 181, 709, 2002
BaeI ACNNNNGTAYC 4 cut(s) 551, 584, 2806, 2839
BanII GRGCYC 2 cut(s) 70, 2060
BarI GAAGNNNNNNTAC 2 cut(s) 597, 629
BauI CACGAG 2 cut(s) 69, 1396
BbsI GAAGAC 6 cut(s) 194, 509, 729, 993, 1934, 2049
Bbv12I GWGCWC 3 cut(s) 70, 290, 2060
BbvI GCAGC 6 cut(s) 98, 129, 480, 664, 1165, 1974
BccI CCATC 7 cut(s) 401, 530, 725, 1916, 2522, 2578, 2798
BceAI ACGGC 5 cut(s) 27, 133, 420, 433, 2704
BcgI CGANNNNNNTGC 2 cut(s) 1721, 1755
BciT130I CCWGG 5 cut(s) 193, 428, 875, 1445, 1591
BciVI GTATCC 2 cut(s) 552, 2112
BclI TGATCA 1 cut(s) 934
BcoDI GTCTC 6 cut(s) 108, 231, 1636, 1732, 2213, 2921
BfmI CTRYAG 4 cut(s) 590, 867, 1284, 1470
BfoI RGCGCY 2 cut(s) 630, 2510
BfuI GTATCC 2 cut(s) 552, 2112
BglII AGATCT 1 cut(s) 2531
BisI GCNGC 9 cut(s) 87, 143, 433, 494, 678, 1179, 1963, 1966, 2766
BlpI GCTNAGC 1 cut(s) 807
BlsI GCNGC 9 cut(s) 88, 144, 434, 495, 679, 1180, 1964, 1967, 2767
Bme1390I CCNGG 5 cut(s) 193, 428, 875, 1445, 1591
Bme18I GGWCC 3 cut(s) 181, 709, 2002
BmeT110I CYCGRG 2 cut(s) 617, 1323
BmgT120I GGNCC 5 cut(s) 181, 424, 700, 709, 2002
BmiI GGNNCC 3 cut(s) 33, 41, 2153
BmrFI CCNGG 5 cut(s) 193, 428, 875, 1445, 1591
BmsI GCATC 6 cut(s) 520, 882, 1225, 1270, 2590, 3052
BmtI GCTAGC 1 cut(s) 215
BoxI GACNNNNGTC 1 cut(s) 240
BpiI GAAGAC 6 cut(s) 194, 509, 729, 993, 1934, 2049
BplI GAGNNNNNCTC 2 cut(s) 2075, 2107
BpmI CTGGAG 3 cut(s) 239, 992, 2235
Bpu10I CCTNAGC 1 cut(s) 2976
Bpu1102I GCTNAGC 1 cut(s) 807
BpuEI CTTGAG 4 cut(s) 725, 870, 1897, 2044
BsaAI YACGTR 1 cut(s) 154
BsaBI GATNNNNATC 1 cut(s) 2547
BsaHI GRCGYC 2 cut(s) 120, 1448
BsaJI CCNNGG 8 cut(s) 35, 192, 426, 427, 1053, 1590, 2147, 2185
BsaXI ACNNNNNCTCC 4 cut(s) 2047, 2077, 2841, 2871
Bsc4I CCNNNNNNNGG 8 cut(s) 116, 412, 718, 1088, 2097, 2341, 2786, 3006
Bse118I RCCGGY 1 cut(s) 93
Bse1I ACTGG 7 cut(s) 222, 681, 1166, 1680, 1963, 2791, 2817
Bse3DI GCAATG 2 cut(s) 2251, 2753
Bse8I GATNNNNATC 1 cut(s) 2547
BseBI CCWGG 5 cut(s) 193, 428, 875, 1445, 1591
BseDI CCNNGG 8 cut(s) 35, 192, 426, 427, 1053, 1590, 2147, 2185
BseGI GGATG 3 cut(s) 486, 1097, 2260
BseJI GATNNNNATC 1 cut(s) 2547
BseLI CCNNNNNNNGG 8 cut(s) 116, 412, 718, 1088, 2097, 2341, 2786, 3006
BseMI GCAATG 2 cut(s) 2251, 2753
BseMII CTCAG 8 cut(s) 90, 318, 384, 450, 687, 1852, 2424, 2967
BseNI ACTGG 7 cut(s) 222, 681, 1166, 1680, 1963, 2791, 2817
BseRI GAGGAG 6 cut(s) 44, 120, 1145, 1367, 2083, 2599
BseX3I CGGCCG 1 cut(s) 433
BseXI GCAGC 6 cut(s) 98, 129, 480, 664, 1165, 1974
BsgI GTGCAG 1 cut(s) 758
Bsh1285I CGRYCG 2 cut(s) 436, 1543
BshFI GGCC 5 cut(s) 425, 435, 701, 1798, 1813
BsiEI CGRYCG 2 cut(s) 436, 1543
BsiHKAI GWGCWC 3 cut(s) 70, 290, 2060
BsiHKCI CYCGRG 2 cut(s) 617, 1323
BsiSI CCGG 2 cut(s) 94, 1795
BsiWI CGTACG 1 cut(s) 176
BslFI GGGAC 4 cut(s) 209, 228, 2250, 3023
BslI CCNNNNNNNGG 8 cut(s) 116, 412, 718, 1088, 2097, 2341, 2786, 3006
BsmAI GTCTC 6 cut(s) 108, 231, 1636, 1732, 2213, 2921
BsmBI CGTCTC 1 cut(s) 108
BsmFI GGGAC 4 cut(s) 209, 228, 2250, 3023
BsmI GAATGC 6 cut(s) 407, 1033, 1501, 1616, 2360, 2733
BsnI GGCC 5 cut(s) 425, 435, 701, 1798, 1813
BsoBI CYCGRG 2 cut(s) 617, 1323
Bsp1286I GDGCHC 3 cut(s) 70, 290, 2060
Bsp1720I GCTNAGC 1 cut(s) 807
Bsp19I CCATGG 1 cut(s) 2185
BspACI CCGC 5 cut(s) 345, 432, 1965, 2766, 3007
BspANI GGCC 5 cut(s) 425, 435, 701, 1798, 1813
BspCNI CTCAG 8 cut(s) 89, 319, 383, 451, 688, 1851, 2423, 2968
BspHI TCATGA 2 cut(s) 2284, 3024
BspLI GGNNCC 3 cut(s) 33, 41, 2153
BspOI GCTAGC 1 cut(s) 215
BspPI GGATC 8 cut(s) 1686, 1942, 2168, 2376, 2537, 2771, 2815, 3025
BsrDI GCAATG 2 cut(s) 2251, 2753
BsrFI RCCGGY 1 cut(s) 93
BsrI ACTGG 7 cut(s) 222, 681, 1166, 1680, 1963, 2791, 2817
BssAI RCCGGY 1 cut(s) 93
BssECI CCNNGG 8 cut(s) 35, 192, 426, 427, 1053, 1590, 2147, 2185
BssNI GRCGYC 2 cut(s) 120, 1448
BssSI CACGAG 2 cut(s) 69, 1396
BssT1I CCWWGG 3 cut(s) 1053, 2147, 2185
Bst2BI CACGAG 2 cut(s) 69, 1396
Bst2UI CCWGG 5 cut(s) 193, 428, 875, 1445, 1591
Bst4CI ACNGT 9 cut(s) 200, 292, 474, 803, 922, 1549, 2138, 2241, 2392
Bst6I CTCTTC 3 cut(s) 1639, 2076, 2961
BstACI GRCGYC 2 cut(s) 120, 1448
BstBAI YACGTR 1 cut(s) 154
BstC8I GCNNGC 6 cut(s) 91, 95, 213, 2211, 2303, 2454
BstDSI CCRYGG 1 cut(s) 2185
BstEII GGTNACC 1 cut(s) 388
BstENI CCTNNNNNAGG 2 cut(s) 1086, 2095
BstF5I GGATG 3 cut(s) 486, 1097, 2260
BstH2I RGCGCY 2 cut(s) 630, 2510
BstHHI GCGC 3 cut(s) 629, 1384, 2509
BstMAI GTCTC 6 cut(s) 108, 231, 1636, 1732, 2213, 2921
BstMCI CGRYCG 2 cut(s) 436, 1543
BstMWI GCNNNNNNNGC 6 cut(s) 212, 1370, 1379, 1505, 2506, 2986
BstNI CCWGG 5 cut(s) 193, 428, 875, 1445, 1591
BstNSI RCATGY 1 cut(s) 2821
BstPAI GACNNNNGTC 1 cut(s) 240
BstPI GGTNACC 1 cut(s) 388
BstSCI CCNGG 5 cut(s) 191, 426, 873, 1443, 1589
BstSFI CTRYAG 4 cut(s) 590, 867, 1284, 1470
BstV1I GCAGC 6 cut(s) 98, 129, 480, 664, 1165, 1974
BstV2I GAAGAC 6 cut(s) 194, 509, 729, 993, 1934, 2049
BstX2I RGATCY 5 cut(s) 1947, 2173, 2531, 2542, 3030
BstYI RGATCY 5 cut(s) 1947, 2173, 2531, 2542, 3030
BstZI CGGCCG 1 cut(s) 433
BsuI GTATCC 2 cut(s) 552, 2112
BsuRI GGCC 5 cut(s) 425, 435, 701, 1798, 1813
BtgI CCRYGG 1 cut(s) 2185
BtsCI GGATG 3 cut(s) 486, 1097, 2260
BtsI GCAGTG 1 cut(s) 2826
Cac8I GCNNGC 6 cut(s) 91, 95, 213, 2211, 2303, 2454
CciI TCATGA 2 cut(s) 2284, 3024
CfoI GCGC 3 cut(s) 629, 1384, 2509
Cfr10I RCCGGY 1 cut(s) 93
Cfr13I GGNCC 5 cut(s) 181, 424, 700, 709, 2002
CseI GACGC 3 cut(s) 109, 245, 1437
Csp6I GTAC 6 cut(s) 151, 177, 438, 2360, 2555, 2823
CspCI CAANNNNNGTGG 2 cut(s) 2932, 2967
CviQI GTAC 6 cut(s) 151, 177, 438, 2360, 2555, 2823
DraI TTTAAA 1 cut(s) 2349
DraIII CACNNNGTG 2 cut(s) 1127, 1420
EaeI YGGCCR 2 cut(s) 433, 1796
EagI CGGCCG 1 cut(s) 433
Eam1104I CTCTTC 3 cut(s) 1639, 2076, 2961
EarI CTCTTC 3 cut(s) 1639, 2076, 2961
Ecl136II GAGCTC 2 cut(s) 68, 2058
EclXI CGGCCG 1 cut(s) 433
Eco130I CCWWGG 3 cut(s) 1053, 2147, 2185
Eco147I AGGCCT 1 cut(s) 1813
Eco24I GRGCYC 2 cut(s) 70, 2060
Eco32I GATATC 1 cut(s) 2842
Eco47I GGWCC 3 cut(s) 181, 709, 2002
Eco52I CGGCCG 1 cut(s) 433
Eco53kI GAGCTC 2 cut(s) 68, 2058
Eco57I CTGAAG 8 cut(s) 182, 206, 521, 1636, 1739, 1928, 1982, 2985
Eco88I CYCGRG 2 cut(s) 617, 1323
Eco91I GGTNACC 1 cut(s) 388
EcoICRI GAGCTC 2 cut(s) 68, 2058
EcoNI CCTNNNNNAGG 2 cut(s) 1086, 2095
EcoO109I RGGNCCY 3 cut(s) 700, 709, 2002
EcoO65I GGTNACC 1 cut(s) 388
EcoRI GAATTC 1 cut(s) 454
EcoRII CCWGG 5 cut(s) 191, 426, 873, 1443, 1589
EcoRV GATATC 1 cut(s) 2842
EcoT14I CCWWGG 3 cut(s) 1053, 2147, 2185
EcoT38I GRGCYC 2 cut(s) 70, 2060
ErhI CCWWGG 3 cut(s) 1053, 2147, 2185
Esp3I CGTCTC 1 cut(s) 108
FalI AAGNNNNNCTT 2 cut(s) 403, 435
FaqI GGGAC 4 cut(s) 209, 228, 2250, 3023
FauI CCCGC 1 cut(s) 3000
FauNDI CATATG 1 cut(s) 1760
FbaI TGATCA 1 cut(s) 934
FblI GTMKAC 1 cut(s) 1865
Fnu4HI GCNGC 9 cut(s) 87, 143, 433, 494, 678, 1179, 1963, 1966, 2766
FokI GGATG 3 cut(s) 473, 1104, 2267
FriOI GRGCYC 2 cut(s) 70, 2060
Fsp4HI GCNGC 9 cut(s) 87, 143, 433, 494, 678, 1179, 1963, 1966, 2766
GlaI GCGC 3 cut(s) 628, 1383, 2508
GluI GCNGC 9 cut(s) 87, 143, 433, 494, 678, 1179, 1963, 1966, 2766
GsuI CTGGAG 3 cut(s) 239, 992, 2235
HaeII RGCGCY 2 cut(s) 630, 2510
HaeIII GGCC 5 cut(s) 425, 435, 701, 1798, 1813
HapII CCGG 2 cut(s) 94, 1795
HgaI GACGC 3 cut(s) 109, 245, 1437
HhaI GCGC 3 cut(s) 629, 1384, 2509
Hin1I GRCGYC 2 cut(s) 120, 1448
Hin6I GCGC 3 cut(s) 627, 1382, 2507
HinP1I GCGC 3 cut(s) 627, 1382, 2507
HincII GTYRAC 4 cut(s) 337, 660, 1042, 2887
HindII GTYRAC 4 cut(s) 337, 660, 1042, 2887
HindIII AAGCTT 3 cut(s) 21, 809, 1703
HpaI GTTAAC 2 cut(s) 337, 1042
HpaII CCGG 2 cut(s) 94, 1795
HphI GGTGA 5 cut(s) 257, 400, 1384, 1898, 1940
Hpy166II GTNNAC 8 cut(s) 337, 587, 660, 1042, 1228, 1525, 1866, 2887
Hpy8I GTNNAC 8 cut(s) 337, 587, 660, 1042, 1228, 1525, 1866, 2887
Hpy99I CGWCG 4 cut(s) 57, 125, 454, 1453
HpyCH4III ACNGT 9 cut(s) 200, 292, 474, 803, 922, 1549, 2138, 2241, 2392
HpyCH4IV ACGT 2 cut(s) 153, 1416
HpyF10VI GCNNNNNNNGC 6 cut(s) 212, 1370, 1379, 1505, 2506, 2986
HpySE526I ACGT 2 cut(s) 153, 1416
Hsp92I GRCGYC 2 cut(s) 120, 1448
HspAI GCGC 3 cut(s) 627, 1382, 2507
KroI GCCGGC 1 cut(s) 93
KroNI GCCGGC 1 cut(s) 95
Ksp22I TGATCA 1 cut(s) 934
KspAI GTTAAC 2 cut(s) 337, 1042
LmnI GCTCC 7 cut(s) 31, 39, 133, 220, 827, 1103, 2055
Lsp1109I GCAGC 6 cut(s) 98, 129, 480, 664, 1165, 1974
LweI GCATC 6 cut(s) 520, 882, 1225, 1270, 2590, 3052
MaeII ACGT 2 cut(s) 153, 1416
MfeI CAATTG 1 cut(s) 2649
MflI RGATCY 5 cut(s) 1947, 2173, 2531, 2542, 3030
MhlI GDGCHC 3 cut(s) 70, 290, 2060
MlyI GAGTC 8 cut(s) 93, 335, 362, 1223, 1818, 2009, 2282, 2402
MmeI TCCRAC 6 cut(s) 390, 699, 938, 1186, 1802, 2608
MreI CGCCGGCG 1 cut(s) 93
MroNI GCCGGC 1 cut(s) 93
MroXI GAANNNNTTC 1 cut(s) 2970
MseI TTAA 8 cut(s) 302, 336, 846, 1041, 1116, 1425, 2319, 2348
MslI CAYNNNNRTG 2 cut(s) 270, 2709
MspA1I CMGCKG 2 cut(s) 493, 1965
MspI CCGG 2 cut(s) 94, 1795
MspR9I CCNGG 5 cut(s) 193, 428, 875, 1445, 1591
MunI CAATTG 1 cut(s) 2649
Mva1269I GAATGC 6 cut(s) 407, 1033, 1501, 1616, 2360, 2733
MvaI CCWGG 5 cut(s) 193, 428, 875, 1445, 1591
MwoI GCNNNNNNNGC 6 cut(s) 212, 1370, 1379, 1505, 2506, 2986
NaeI GCCGGC 1 cut(s) 95
NcoI CCATGG 1 cut(s) 2185
NdeI CATATG 1 cut(s) 1760
NgoMIV GCCGGC 1 cut(s) 93
NheI GCTAGC 1 cut(s) 211
NlaIV GGNNCC 3 cut(s) 33, 41, 2153
NmeAIII GCCGAG 1 cut(s) 2642
NmuCI GTSAC 7 cut(s) 233, 388, 1339, 1646, 1709, 1916, 2065
NspI RCATGY 1 cut(s) 2821
OliI CACNNNNGTG 1 cut(s) 270
PaeR7I CTCGAG 2 cut(s) 617, 1323
PagI TCATGA 2 cut(s) 2284, 3024
PasI CCCWGGG 1 cut(s) 427
PceI AGGCCT 1 cut(s) 1813
PcsI WCGNNNNNNNCGW 2 cut(s) 61, 449
PctI GAATGC 6 cut(s) 407, 1033, 1501, 1616, 2360, 2733
PdiI GCCGGC 1 cut(s) 95
PdmI GAANNNNTTC 1 cut(s) 2970
Pfl23II CGTACG 1 cut(s) 176
PflFI GACNNNGTC 1 cut(s) 119
PkrI GCNGC 9 cut(s) 88, 144, 434, 495, 679, 1180, 1964, 1967, 2767
Ple19I CGATCG 1 cut(s) 1543
PleI GAGTC 8 cut(s) 93, 335, 362, 1223, 1818, 2009, 2282, 2402
PpsI GAGTC 8 cut(s) 93, 335, 362, 1223, 1818, 2009, 2282, 2402
Ppu21I YACGTR 1 cut(s) 154
PpuMI RGGWCCY 2 cut(s) 709, 2002
PshAI GACNNNNGTC 1 cut(s) 240
PsiI TTATAA 1 cut(s) 410
Psp124BI GAGCTC 2 cut(s) 70, 2060
Psp5II RGGWCCY 2 cut(s) 709, 2002
Psp6I CCWGG 5 cut(s) 191, 426, 873, 1443, 1589
PspEI GGTNACC 1 cut(s) 388
PspGI CCWGG 5 cut(s) 191, 426, 873, 1443, 1589
PspLI CGTACG 1 cut(s) 176
PspN4I GGNNCC 3 cut(s) 33, 41, 2153
PspPI GGNCC 5 cut(s) 181, 424, 700, 709, 2002
PspPPI RGGWCCY 2 cut(s) 709, 2002
PsuI RGATCY 5 cut(s) 1947, 2173, 2531, 2542, 3030
PsyI GACNNNGTC 1 cut(s) 119
PvuI CGATCG 1 cut(s) 1543
PvuII CAGCTG 1 cut(s) 493
RsaI GTAC 6 cut(s) 152, 178, 439, 2361, 2556, 2824
RsaNI GTAC 6 cut(s) 151, 177, 438, 2360, 2555, 2823
RseI CAYNNNNRTG 2 cut(s) 270, 2709
SacI GAGCTC 2 cut(s) 70, 2060
SaqAI TTAA 8 cut(s) 302, 336, 846, 1041, 1116, 1425, 2319, 2348
SatI GCNGC 9 cut(s) 87, 143, 433, 494, 678, 1179, 1963, 1966, 2766
Sau96I GGNCC 5 cut(s) 181, 424, 700, 709, 2002
SchI GAGTC 8 cut(s) 93, 335, 362, 1223, 1818, 2009, 2282, 2402
ScrFI CCNGG 5 cut(s) 193, 428, 875, 1445, 1591
SduI GDGCHC 3 cut(s) 70, 290, 2060
SfaNI GCATC 6 cut(s) 520, 882, 1225, 1270, 2590, 3052
SfcI CTRYAG 4 cut(s) 590, 867, 1284, 1470
Sfr274I CTCGAG 2 cut(s) 617, 1323
SgrAI CRCCGGYG 1 cut(s) 93
SinI GGWCC 3 cut(s) 181, 709, 2002
SlaI CTCGAG 2 cut(s) 617, 1323
SmiMI CAYNNNNRTG 2 cut(s) 270, 2709
SmlI CTYRAG 6 cut(s) 617, 704, 885, 1323, 1912, 2059
SmoI CTYRAG 6 cut(s) 617, 704, 885, 1323, 1912, 2059
SseBI AGGCCT 1 cut(s) 1813
SsiI CCGC 5 cut(s) 345, 432, 1965, 2766, 3007
SstI GAGCTC 2 cut(s) 70, 2060
StuI AGGCCT 1 cut(s) 1813
StyD4I CCNGG 5 cut(s) 191, 426, 873, 1443, 1589
StyI CCWWGG 3 cut(s) 1053, 2147, 2185
TaaI ACNGT 9 cut(s) 200, 292, 474, 803, 922, 1549, 2138, 2241, 2392
TaiI ACGT 2 cut(s) 156, 1419
TauI GCSGC 3 cut(s) 435, 1968, 2768
Tru1I TTAA 8 cut(s) 302, 336, 846, 1041, 1116, 1425, 2319, 2348
Tru9I TTAA 8 cut(s) 302, 336, 846, 1041, 1116, 1425, 2319, 2348
TseFI GTSAC 7 cut(s) 233, 388, 1339, 1646, 1709, 1916, 2065
TseI GCWGC 6 cut(s) 86, 142, 493, 677, 1178, 1962
Tsp45I GTSAC 7 cut(s) 233, 388, 1339, 1646, 1709, 1916, 2065
TspGWI ACGGA 5 cut(s) 194, 764, 1785, 2572, 2825
Tth111I GACNNNGTC 1 cut(s) 119
VpaK11BI GGWCC 3 cut(s) 181, 709, 2002
XagI CCTNNNNNAGG 2 cut(s) 1086, 2095
XapI RAATTY 7 cut(s) 454, 541, 665, 1661, 1802, 2426, 2615
XceI RCATGY 1 cut(s) 2821
XcmI CCANNNNNNNNNTGG 1 cut(s) 253
XhoI CTCGAG 2 cut(s) 617, 1323
XmiI GTMKAC 1 cut(s) 1865
XmnI GAANNNNTTC 1 cut(s) 2970
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.