RchiOBHm_Chr2g0170071

OST-HTH/LOTUS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000679
Physical Location & Seq
Forward (+)
84084631 .. 84085307
677 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53763

Sequence Viewer

Length: 528 bp
ATGTCACCTCAACCACAAGATCAAGAGAAGAGTTTTCAATACCAGCTTCCACTGCAGAAGCCGAACGAGAATATTGTGGAAGGTGATAACCCTGAACAAAGTTCTTGGGCTGATAGATTAGTCCTTAAACAACCAGCAGATTGTTCCATTGAACCTCAAGCGCATGTTACCAAGAGCTCTGAGGACCCAAGCACTCCTACATGGCTAAAGGTTTTCAACAAGTGGTTTCCTGGGTTCTTACAAGGGCTGTCAAAGCATTCAAATTATGCTCTCTCTTCTCTAAAAGCTGACTTCAAGGCTAAATTTGGGTTAGAACTGGATCACTCATCTGTGGGCTACTCTAAGCTCAGAGACTTCCTGAAGCGTGTCTCTGATTTATGTACCATCGAGATTCTCCCCATATGCAAACATGGAACTCCAACTCACATGATCCTCCGTCCAAAATTTTCTAGTCCTCAATGCCGCCTCCTTCCTACACTCAGAACAACTCACACTGTCTTTGTCTGCATTTATTGTGAATGGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 OST-HTH RRM_1

Protein Analysis

175

Amino Acids

20.08

Weight (kDa)

8.2

Isoelectric Point (pI)

59.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OST-HTH PF12872 71 - 130 1e-08 OST-HTH/LOTUS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 463
AclWI GGATC 2 cut(s) 327, 424
AcsI RAATTY 2 cut(s) 302, 443
AcuI CTGAAG 1 cut(s) 380
AfaI GTAC 1 cut(s) 382
AgsI TTSAA 5 cut(s) 38, 152, 217, 261, 295
AjnI CCWGG 1 cut(s) 229
AluBI AGCT 4 cut(s) 46, 177, 287, 346
AluI AGCT 4 cut(s) 46, 177, 287, 346
Alw21I GWGCWC 1 cut(s) 179
Alw26I GTCTC 2 cut(s) 345, 373
AlwI GGATC 2 cut(s) 327, 424
ApoI RAATTY 2 cut(s) 302, 443
AspLEI GCGC 1 cut(s) 163
AspS9I GGNCC 1 cut(s) 184
AsuHPI GGTGA 1 cut(s) 95
AvaII GGWCC 1 cut(s) 184
BanII GRGCYC 1 cut(s) 179
Bbv12I GWGCWC 1 cut(s) 179
BccI CCATC 1 cut(s) 392
BciT130I CCWGG 1 cut(s) 231
BcoDI GTCTC 2 cut(s) 345, 373
BfaI CTAG 1 cut(s) 450
BfmI CTRYAG 1 cut(s) 53
BisI GCNGC 1 cut(s) 463
BlsI GCNGC 1 cut(s) 464
Bme1390I CCNGG 1 cut(s) 231
Bme18I GGWCC 1 cut(s) 184
BmgT120I GGNCC 1 cut(s) 184
BmiI GGNNCC 1 cut(s) 186
BmrFI CCNGG 1 cut(s) 231
BpuEI CTTGAG 1 cut(s) 141
BsaJI CCNNGG 1 cut(s) 230
Bse1I ACTGG 1 cut(s) 321
BseBI CCWGG 1 cut(s) 231
BseDI CCNNGG 1 cut(s) 230
BseMII CTCAG 3 cut(s) 171, 361, 493
BseNI ACTGG 1 cut(s) 321
BsiHKAI GWGCWC 1 cut(s) 179
BsmAI GTCTC 2 cut(s) 345, 373
BsmI GAATGC 1 cut(s) 256
Bsp1286I GDGCHC 1 cut(s) 179
Bsp143I GATC 3 cut(s) 19, 319, 429
BspACI CCGC 1 cut(s) 463
BspCNI CTCAG 3 cut(s) 172, 360, 492
BspLI GGNNCC 1 cut(s) 186
BspMAI CTGCAG 1 cut(s) 57
BspPI GGATC 2 cut(s) 327, 424
BsrI ACTGG 1 cut(s) 321
BssECI CCNNGG 1 cut(s) 230
BssMI GATC 3 cut(s) 19, 319, 429
Bst2UI CCWGG 1 cut(s) 231
Bst4CI ACNGT 1 cut(s) 496
Bst6I CTCTTC 2 cut(s) 23, 280
BstDEI CTNAG 4 cut(s) 180, 342, 347, 479
BstHHI GCGC 1 cut(s) 163
BstKTI GATC 3 cut(s) 22, 322, 432
BstMAI GTCTC 2 cut(s) 345, 373
BstMBI GATC 3 cut(s) 19, 319, 429
BstMWI GCNNNNNNNGC 2 cut(s) 52, 253
BstNI CCWGG 1 cut(s) 231
BstNSI RCATGY 1 cut(s) 167
BstSCI CCNGG 1 cut(s) 229
BstSFI CTRYAG 1 cut(s) 53
BtsI GCAGTG 1 cut(s) 50
BtsIMutI CAGTG 2 cut(s) 50, 492
CfoI GCGC 1 cut(s) 163
Cfr13I GGNCC 1 cut(s) 184
Csp6I GTAC 1 cut(s) 381
CviAII CATG 4 cut(s) 164, 201, 410, 427
CviQI GTAC 1 cut(s) 381
DdeI CTNAG 4 cut(s) 180, 342, 347, 479
DpnI GATC 3 cut(s) 21, 321, 431
DpnII GATC 3 cut(s) 19, 319, 429
Eam1104I CTCTTC 2 cut(s) 23, 280
EarI CTCTTC 2 cut(s) 23, 280
Ecl136II GAGCTC 1 cut(s) 177
Eco24I GRGCYC 1 cut(s) 179
Eco47I GGWCC 1 cut(s) 184
Eco53kI GAGCTC 1 cut(s) 177
Eco57I CTGAAG 1 cut(s) 380
EcoICRI GAGCTC 1 cut(s) 177
EcoO109I RGGNCCY 1 cut(s) 184
EcoRII CCWGG 1 cut(s) 229
EcoT38I GRGCYC 1 cut(s) 179
FaeI CATG 4 cut(s) 167, 204, 413, 430
FaiI YATR 8 cut(s) 165, 202, 267, 379, 401, 403, 411, 428
FatI CATG 4 cut(s) 163, 200, 409, 426
FauNDI CATATG 1 cut(s) 401
Fnu4HI GCNGC 1 cut(s) 463
FriOI GRGCYC 1 cut(s) 179
Fsp4HI GCNGC 1 cut(s) 463
FspBI CTAG 1 cut(s) 450
GlaI GCGC 1 cut(s) 162
GluI GCNGC 1 cut(s) 463
HhaI GCGC 1 cut(s) 163
Hin1II CATG 4 cut(s) 167, 204, 413, 430
Hin6I GCGC 1 cut(s) 161
HinP1I GCGC 1 cut(s) 161
HinfI GANTC 1 cut(s) 391
HphI GGTGA 1 cut(s) 95
Hpy188I TCNGA 4 cut(s) 181, 350, 373, 482
Hpy188III TCNNGA 3 cut(s) 23, 358, 388
HpyAV CCTTC 2 cut(s) 74, 479
HpyCH4III ACNGT 1 cut(s) 496
HpyCH4V TGCA 3 cut(s) 55, 405, 507
HpyF10VI GCNNNNNNNGC 2 cut(s) 52, 253
HpyF3I CTNAG 4 cut(s) 180, 342, 347, 479
Hsp92II CATG 4 cut(s) 167, 204, 413, 430
HspAI GCGC 1 cut(s) 161
Kzo9I GATC 3 cut(s) 19, 319, 429
LpnPI CCDG 7 cut(s) 56, 105, 147, 216, 243, 302, 371
MaeI CTAG 1 cut(s) 450
MaeIII GTNAC 2 cut(s) 3, 166
MalI GATC 3 cut(s) 21, 321, 431
MboI GATC 3 cut(s) 19, 319, 429
MboII GAAGA 2 cut(s) 40, 267
MhlI GDGCHC 1 cut(s) 179
MluCI AATT 3 cut(s) 262, 302, 443
MmeI TCCRAC 1 cut(s) 443
MnlI CCTC 6 cut(s) 18, 165, 175, 443, 465, 476
MseI TTAA 1 cut(s) 126
MspR9I CCNGG 1 cut(s) 231
Mva1269I GAATGC 1 cut(s) 256
MvaI CCWGG 1 cut(s) 231
MwoI GCNNNNNNNGC 2 cut(s) 52, 253
NdeI CATATG 1 cut(s) 401
NdeII GATC 3 cut(s) 19, 319, 429
NlaIII CATG 4 cut(s) 167, 204, 413, 430
NlaIV GGNNCC 1 cut(s) 186
NmuCI GTSAC 1 cut(s) 3
NspI RCATGY 1 cut(s) 167
PctI GAATGC 1 cut(s) 256
PfeI GAWTC 1 cut(s) 391
PkrI GCNGC 1 cut(s) 464
PpuMI RGGWCCY 1 cut(s) 184
Psp124BI GAGCTC 1 cut(s) 179
Psp5II RGGWCCY 1 cut(s) 184
Psp6I CCWGG 1 cut(s) 229
PspGI CCWGG 1 cut(s) 229
PspN4I GGNNCC 1 cut(s) 186
PspPI GGNCC 1 cut(s) 184
PspPPI RGGWCCY 1 cut(s) 184
PstI CTGCAG 1 cut(s) 57
RsaI GTAC 1 cut(s) 382
RsaNI GTAC 1 cut(s) 381
SacI GAGCTC 1 cut(s) 179
SaqAI TTAA 1 cut(s) 126
SatI GCNGC 1 cut(s) 463
Sau3AI GATC 3 cut(s) 19, 319, 429
Sau96I GGNCC 1 cut(s) 184
ScrFI CCNGG 1 cut(s) 231
SduI GDGCHC 1 cut(s) 179
SetI ASST 8 cut(s) 10, 48, 85, 157, 179, 213, 289, 348
SfcI CTRYAG 1 cut(s) 53
SinI GGWCC 1 cut(s) 184
SmlI CTYRAG 1 cut(s) 156
SmoI CTYRAG 1 cut(s) 156
Sse9I AATT 3 cut(s) 262, 302, 443
SsiI CCGC 1 cut(s) 463
SspI AATATT 1 cut(s) 73
SspMI CTAG 1 cut(s) 450
SstI GAGCTC 1 cut(s) 179
StyD4I CCNGG 1 cut(s) 229
TaaI ACNGT 1 cut(s) 496
TaqI TCGA 1 cut(s) 387
TasI AATT 3 cut(s) 262, 302, 443
TauI GCSGC 1 cut(s) 465
TfiI GAWTC 1 cut(s) 391
Tru1I TTAA 1 cut(s) 126
Tru9I TTAA 1 cut(s) 126
TscAI CASTG 2 cut(s) 57, 499
TseFI GTSAC 1 cut(s) 3
Tsp45I GTSAC 1 cut(s) 3
TspGWI ACGGA 1 cut(s) 425
TspRI CASTG 2 cut(s) 57, 499
VpaK11BI GGWCC 1 cut(s) 184
XapI RAATTY 2 cut(s) 302, 443
XceI RCATGY 1 cut(s) 167
XspI CTAG 1 cut(s) 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.