Rh2BG620800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000679
Physical Location & Seq
Forward (+)
84356169 .. 84358064
1896 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG620800.1

Sequence Viewer

Length: 471 bp
ATGGAAAAACTGAAAGCTAAGGTGAAGTACGTACCAGCTGGCATGGTCAAAACCCAAGTATTTACGAAACCGCTTGTGGCTCAAACGAAGCGGGAAAAACGAAGCTTCGGAGCTCTTCAACTCGTCTCCGAGTGTGACCAGAGCCGTCTATCTAGACGACAAGTACGAGCTCGTGGCTCAGATGAAGCGGCTCGCCGGCGACTCGCTGAACCGAGAGACGGCGTCGAAGCTCCTCCACCTCTGCTGCAAGTTCGACTTGCCGGAGTGCGCCTCTGCTCTTCTCGGCGGAGAACTCGGGACGATTCCTCTAGTGAACGAGTTGGACGACTTGGGGAGATAGCCGCTGCACACGGCGGCGTTGGCTCACTCGTCGCTGTGCGTAGATGTACGTATGTTGCTGAAGAAACACGCTCGTACGAACCTGAAGACCAGGGACGGTCAGGCACAGCTAGCACTGGAGCTGTCGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

156

Amino Acids

17.05

Weight (kDa)

10.5

Isoelectric Point (pI)

74.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 71, 91, 188, 286, 342, 354
AcuI CTGAAG 2 cut(s) 420, 444
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 5 cut(s) 29, 33, 165, 388, 416
AfiI CCNNNNNNNGG 1 cut(s) 218
AgsI TTSAA 1 cut(s) 119
AjnI CCWGG 1 cut(s) 429
AluBI AGCT 8 cut(s) 17, 38, 105, 113, 170, 230, 449, 461
AluI AGCT 8 cut(s) 17, 38, 105, 113, 170, 230, 449, 461
Alw21I GWGCWC 2 cut(s) 115, 172
Alw26I GTCTC 2 cut(s) 130, 210
Ama87I CYCGRG 1 cut(s) 294
ApeKI GCWGC 2 cut(s) 244, 344
AspLEI GCGC 1 cut(s) 270
AsuHPI GGTGA 1 cut(s) 34
AsuNHI GCTAGC 1 cut(s) 449
AvaI CYCGRG 1 cut(s) 294
BanII GRGCYC 2 cut(s) 115, 172
BauI CACGAG 1 cut(s) 171
BbsI GAAGAC 1 cut(s) 432
Bbv12I GWGCWC 2 cut(s) 115, 172
BbvI GCAGC 2 cut(s) 231, 331
BceAI ACGGC 3 cut(s) 129, 235, 367
BciT130I CCWGG 1 cut(s) 431
BcoDI GTCTC 2 cut(s) 130, 210
BfaI CTAG 3 cut(s) 153, 309, 450
BisI GCNGC 5 cut(s) 189, 245, 342, 345, 355
BlsI GCNGC 5 cut(s) 190, 246, 343, 346, 356
Bme1390I CCNGG 1 cut(s) 431
BmeT110I CYCGRG 1 cut(s) 294
BmrFI CCNGG 1 cut(s) 431
BmtI GCTAGC 1 cut(s) 453
BpiI GAAGAC 1 cut(s) 432
BplI GAGNNNNNCTC 2 cut(s) 255, 287
Bpu10I CCTNAGC 1 cut(s) 18
BsaAI YACGTR 2 cut(s) 31, 390
BsaHI GRCGYC 1 cut(s) 222
BsaJI CCNNGG 1 cut(s) 430
Bsc4I CCNNNNNNNGG 1 cut(s) 218
Bse118I RCCGGY 1 cut(s) 195
Bse1I ACTGG 1 cut(s) 460
BseBI CCWGG 1 cut(s) 431
BseDI CCNNGG 1 cut(s) 430
BseLI CCNNNNNNNGG 1 cut(s) 218
BseMII CTCAG 1 cut(s) 192
BseNI ACTGG 1 cut(s) 460
BseRI GAGGAG 1 cut(s) 222
BseXI GCAGC 2 cut(s) 231, 331
BsgI GTGCAG 1 cut(s) 330
BsiHKAI GWGCWC 2 cut(s) 115, 172
BsiHKCI CYCGRG 1 cut(s) 294
BsiSI CCGG 2 cut(s) 196, 261
BsiWI CGTACG 1 cut(s) 414
BslFI GGGAC 2 cut(s) 311, 447
BslI CCNNNNNNNGG 1 cut(s) 218
BsmAI GTCTC 2 cut(s) 130, 210
BsmBI CGTCTC 2 cut(s) 130, 210
BsmFI GGGAC 2 cut(s) 311, 447
BsoBI CYCGRG 1 cut(s) 294
Bsp1286I GDGCHC 2 cut(s) 115, 172
BspACI CCGC 6 cut(s) 71, 91, 188, 286, 342, 354
BspCNI CTCAG 1 cut(s) 191
BspOI GCTAGC 1 cut(s) 453
BspQI GCTCTTC 2 cut(s) 120, 283
BsrFI RCCGGY 1 cut(s) 195
BsrI ACTGG 1 cut(s) 460
BssAI RCCGGY 1 cut(s) 195
BssECI CCNNGG 1 cut(s) 430
BssNI GRCGYC 1 cut(s) 222
BssSI CACGAG 1 cut(s) 171
Bst2BI CACGAG 1 cut(s) 171
Bst2UI CCWGG 1 cut(s) 431
Bst4CI ACNGT 1 cut(s) 438
Bst6I CTCTTC 2 cut(s) 120, 283
BstACI GRCGYC 1 cut(s) 222
BstBAI YACGTR 2 cut(s) 31, 390
BstC8I GCNNGC 4 cut(s) 40, 193, 197, 451
BstDEI CTNAG 3 cut(s) 18, 178, 468
BstHHI GCGC 1 cut(s) 270
BstMAI GTCTC 2 cut(s) 130, 210
BstMWI GCNNNNNNNGC 2 cut(s) 360, 450
BstNI CCWGG 1 cut(s) 431
BstSCI CCNGG 1 cut(s) 429
BstSNI TACGTA 2 cut(s) 31, 390
BstV1I GCAGC 2 cut(s) 231, 331
BstV2I GAAGAC 1 cut(s) 432
BtsIMutI CAGTG 1 cut(s) 453
Cac8I GCNNGC 4 cut(s) 40, 193, 197, 451
CfoI GCGC 1 cut(s) 270
Cfr10I RCCGGY 1 cut(s) 195
CseI GACGC 1 cut(s) 211
Csp6I GTAC 5 cut(s) 28, 32, 164, 387, 415
CviAII CATG 1 cut(s) 43
CviQI GTAC 5 cut(s) 28, 32, 164, 387, 415
DdeI CTNAG 3 cut(s) 18, 178, 468
Eam1104I CTCTTC 2 cut(s) 120, 283
EarI CTCTTC 2 cut(s) 120, 283
EciI GGCGGA 1 cut(s) 301
Ecl136II GAGCTC 2 cut(s) 113, 170
Eco105I TACGTA 2 cut(s) 31, 390
Eco24I GRGCYC 2 cut(s) 115, 172
Eco53kI GAGCTC 2 cut(s) 113, 170
Eco57I CTGAAG 2 cut(s) 420, 444
Eco88I CYCGRG 1 cut(s) 294
EcoICRI GAGCTC 2 cut(s) 113, 170
EcoRII CCWGG 1 cut(s) 429
EcoT38I GRGCYC 2 cut(s) 115, 172
Esp3I CGTCTC 2 cut(s) 130, 210
FaeI CATG 1 cut(s) 46
FaiI YATR 2 cut(s) 44, 393
FalI AAGNNNNNCTT 2 cut(s) 240, 272
FaqI GGGAC 2 cut(s) 311, 447
FatI CATG 1 cut(s) 42
FauI CCCGC 1 cut(s) 84
Fnu4HI GCNGC 5 cut(s) 189, 245, 342, 345, 355
FriOI GRGCYC 2 cut(s) 115, 172
Fsp4HI GCNGC 5 cut(s) 189, 245, 342, 345, 355
FspBI CTAG 3 cut(s) 153, 309, 450
GlaI GCGC 1 cut(s) 269
GluI GCNGC 5 cut(s) 189, 245, 342, 345, 355
HapII CCGG 2 cut(s) 196, 261
HgaI GACGC 1 cut(s) 211
HhaI GCGC 1 cut(s) 270
Hin1I GRCGYC 1 cut(s) 222
Hin1II CATG 1 cut(s) 46
Hin6I GCGC 1 cut(s) 268
HinP1I GCGC 1 cut(s) 268
HindIII AAGCTT 1 cut(s) 103
HinfI GANTC 2 cut(s) 201, 302
HpaII CCGG 2 cut(s) 196, 261
HphI GGTGA 1 cut(s) 34
Hpy166II GTNNAC 1 cut(s) 314
Hpy188I TCNGA 3 cut(s) 110, 130, 181
Hpy188III TCNNGA 2 cut(s) 153, 296
Hpy8I GTNNAC 1 cut(s) 314
Hpy99I CGWCG 2 cut(s) 227, 374
HpyCH4III ACNGT 1 cut(s) 438
HpyCH4IV ACGT 2 cut(s) 30, 389
HpyCH4V TGCA 2 cut(s) 247, 347
HpyF10VI GCNNNNNNNGC 2 cut(s) 360, 450
HpyF3I CTNAG 3 cut(s) 18, 178, 468
HpySE526I ACGT 2 cut(s) 30, 389
Hsp92I GRCGYC 1 cut(s) 222
Hsp92II CATG 1 cut(s) 46
HspAI GCGC 1 cut(s) 268
KroI GCCGGC 1 cut(s) 195
KroNI GCCGGC 1 cut(s) 197
LguI GCTCTTC 2 cut(s) 120, 283
LmnI GCTCC 3 cut(s) 110, 235, 458
Lsp1109I GCAGC 2 cut(s) 231, 331
MaeI CTAG 3 cut(s) 153, 309, 450
MaeII ACGT 2 cut(s) 30, 389
MaeIII GTNAC 1 cut(s) 134
MboII GAAGA 4 cut(s) 107, 270, 413, 437
MhlI GDGCHC 2 cut(s) 115, 172
MlyI GAGTC 1 cut(s) 195
MmeI TCCRAC 1 cut(s) 301
MnlI CCTC 4 cut(s) 243, 249, 281, 316
MreI CGCCGGCG 1 cut(s) 195
MroNI GCCGGC 1 cut(s) 195
MspA1I CMGCKG 2 cut(s) 38, 344
MspI CCGG 2 cut(s) 196, 261
MspR9I CCNGG 1 cut(s) 431
MvaI CCWGG 1 cut(s) 431
MwoI GCNNNNNNNGC 2 cut(s) 360, 450
NaeI GCCGGC 1 cut(s) 197
NgoMIV GCCGGC 1 cut(s) 195
NheI GCTAGC 1 cut(s) 449
NlaIII CATG 1 cut(s) 46
NmeAIII GCCGAG 1 cut(s) 262
NmuCI GTSAC 1 cut(s) 134
PciSI GCTCTTC 2 cut(s) 120, 283
PcsI WCGNNNNNNNCGW 2 cut(s) 163, 322
PdiI GCCGGC 1 cut(s) 197
PfeI GAWTC 1 cut(s) 302
Pfl23II CGTACG 1 cut(s) 414
PflFI GACNNNGTC 1 cut(s) 221
PkrI GCNGC 5 cut(s) 190, 246, 343, 346, 356
PleI GAGTC 1 cut(s) 195
PpsI GAGTC 1 cut(s) 195
Ppu21I YACGTR 2 cut(s) 31, 390
Psp124BI GAGCTC 2 cut(s) 115, 172
Psp6I CCWGG 1 cut(s) 429
PspGI CCWGG 1 cut(s) 429
PspLI CGTACG 1 cut(s) 414
PsyI GACNNNGTC 1 cut(s) 221
PvuII CAGCTG 1 cut(s) 38
RsaI GTAC 5 cut(s) 29, 33, 165, 388, 416
RsaNI GTAC 5 cut(s) 28, 32, 164, 387, 415
SacI GAGCTC 2 cut(s) 115, 172
SapI GCTCTTC 2 cut(s) 120, 283
SatI GCNGC 5 cut(s) 189, 245, 342, 345, 355
SchI GAGTC 1 cut(s) 195
ScrFI CCNGG 1 cut(s) 431
SduI GDGCHC 2 cut(s) 115, 172
SgrAI CRCCGGYG 1 cut(s) 195
SnaBI TACGTA 2 cut(s) 31, 390
SsiI CCGC 6 cut(s) 71, 91, 188, 286, 342, 354
SspMI CTAG 3 cut(s) 153, 309, 450
SstI GAGCTC 2 cut(s) 115, 172
StyD4I CCNGG 1 cut(s) 429
TaaI ACNGT 1 cut(s) 438
TaiI ACGT 2 cut(s) 33, 392
TaqI TCGA 2 cut(s) 225, 253
TauI GCSGC 3 cut(s) 191, 344, 357
TfiI GAWTC 1 cut(s) 302
TscAI CASTG 1 cut(s) 460
TseFI GTSAC 1 cut(s) 134
TseI GCWGC 2 cut(s) 244, 344
Tsp45I GTSAC 1 cut(s) 134
TspDTI ATGAA 1 cut(s) 198
TspRI CASTG 1 cut(s) 460
Tth111I GACNNNGTC 1 cut(s) 221
XbaI TCTAGA 1 cut(s) 152
XspI CTAG 3 cut(s) 153, 309, 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.