RchiOBHm_Chr6g0246231

OST-HTH/LOTUS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000679
Physical Location & Seq
Forward (+)
1971022 .. 1972088
1067 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22071

Sequence Viewer

Length: 570 bp
ATGGTTCCGGTTCGTCGTGAGCGGTCCGATTCAGATCCGAGTTCCGCCTGTGACCTCCGACACGATGCGAGTGAAGACCCAATCGACTCGGTCGAGGCCGAGACGAGTCTGATCAATGCCGTCTACCTCGACAGCAAGGCCGAGCTCGTGGCTCAGTCGAAGCGACTCGGCGGCCGGGCGTTAGACCTAGCGACCGCGTCGAAGCTCCTGCATCTCTGCTGCAACTTTGACTCTGTAGAGTGCGCCTCGGCGCTTCTCAGCGGACAGCTTGGAGCGATACCACTCGTGAACGAGTTAGACGAGGCCGGGAAAACGCCGCTACACACGGCGGTGGAGTCTCACGCGTTGCGGTGCGTGGAGGCGTTGCTGAAGAAACACGCTCGTACGGACCTGAGGACGAAGGACGGTGAGGCACAGCTACCGTTGGAGTTGTCGCTTACTGAGGCAAGGTTGGATATATATTGGAATCCGAACGATTTCTCCAAGGAAGAATTTGTCCTTCACATAACTGAAAAGGATTTGAGCATTGTGAGACTTTTGAGTGACAAAACCAAGGAGATCGCTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 OST-HTH RRM_1

Protein Analysis

189

Amino Acids

20.78

Weight (kDa)

4.95

Isoelectric Point (pI)

44.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 66 - 130 4.7e-09 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 22
AccI GTMKAC 1 cut(s) 123
AccII CGCG 2 cut(s) 197, 344
AciI CCGC 8 cut(s) 22, 45, 171, 195, 261, 317, 329, 349
AclWI GGATC 1 cut(s) 29
AcoI YGGCCR 1 cut(s) 172
AcsI RAATTY 1 cut(s) 491
AcuI CTGAAG 1 cut(s) 389
AfaI GTAC 1 cut(s) 385
AflIII ACRYGT 1 cut(s) 342
AleI CACNNNNGTG 1 cut(s) 329
AloI GAACNNNNNNTCC 2 cut(s) 464, 496
AluBI AGCT 4 cut(s) 145, 205, 268, 418
AluI AGCT 4 cut(s) 145, 205, 268, 418
Alw21I GWGCWC 1 cut(s) 147
Alw26I GTCTC 3 cut(s) 95, 342, 526
AlwI GGATC 1 cut(s) 29
AoxI GGCC 4 cut(s) 96, 138, 172, 303
ApeKI GCWGC 1 cut(s) 219
ApoI RAATTY 1 cut(s) 491
Asp700I GAANNNNTTC 1 cut(s) 476
AspLEI GCGC 2 cut(s) 245, 253
AspS9I GGNCC 2 cut(s) 24, 388
AsuC2I CCSGG 2 cut(s) 176, 307
AsuHPI GGTGA 1 cut(s) 419
AvaII GGWCC 2 cut(s) 24, 388
AxyI CCTNAGG 1 cut(s) 392
BanII GRGCYC 1 cut(s) 147
BauI CACGAG 2 cut(s) 146, 284
BbsI GAAGAC 1 cut(s) 81
Bbv12I GWGCWC 1 cut(s) 147
BbvI GCAGC 1 cut(s) 206
BceAI ACGGC 2 cut(s) 104, 342
BcgI CGANNNNNNTGC 2 cut(s) 190, 224
BclI TGATCA 1 cut(s) 111
BcnI CCSGG 2 cut(s) 176, 307
BcoDI GTCTC 3 cut(s) 95, 342, 526
BfaI CTAG 1 cut(s) 188
BfmI CTRYAG 1 cut(s) 234
BfoI RGCGCY 1 cut(s) 254
BisI GCNGC 3 cut(s) 172, 220, 317
BlsI GCNGC 3 cut(s) 173, 221, 318
Bme1390I CCNGG 2 cut(s) 176, 307
Bme18I GGWCC 2 cut(s) 24, 388
BmgT120I GGNCC 2 cut(s) 24, 388
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 2 cut(s) 176, 307
BmsI GCATC 2 cut(s) 55, 220
BpiI GAAGAC 1 cut(s) 81
BplI GAGNNNNNCTC 2 cut(s) 230, 262
BpuMI CCSGG 2 cut(s) 176, 307
BsaBI GATNNNNATC 1 cut(s) 33
BsaJI CCNNGG 3 cut(s) 246, 483, 552
BsaWI WCCGGW 1 cut(s) 7
BsaXI ACNNNNNCTCC 2 cut(s) 464, 494
Bse21I CCTNAGG 1 cut(s) 392
Bse8I GATNNNNATC 1 cut(s) 33
BseDI CCNNGG 3 cut(s) 246, 483, 552
BseJI GATNNNNATC 1 cut(s) 33
BseMII CTCAG 5 cut(s) 167, 271, 383, 432, 555
BseX3I CGGCCG 1 cut(s) 172
BseXI GCAGC 1 cut(s) 206
Bsh1236I CGCG 2 cut(s) 197, 344
Bsh1285I CGRYCG 3 cut(s) 93, 175, 195
BshFI GGCC 4 cut(s) 98, 140, 174, 305
BsiEI CGRYCG 3 cut(s) 93, 175, 195
BsiHKAI GWGCWC 1 cut(s) 147
BsiSI CCGG 3 cut(s) 8, 175, 306
BsiWI CGTACG 1 cut(s) 383
BsmAI GTCTC 3 cut(s) 95, 342, 526
BsmBI CGTCTC 1 cut(s) 95
BsnI GGCC 4 cut(s) 98, 140, 174, 305
Bsp1286I GDGCHC 1 cut(s) 147
Bsp143I GATC 3 cut(s) 34, 111, 558
BspACI CCGC 8 cut(s) 22, 45, 171, 195, 261, 317, 329, 349
BspANI GGCC 4 cut(s) 98, 140, 174, 305
BspCNI CTCAG 5 cut(s) 166, 270, 384, 433, 556
BspFNI CGCG 2 cut(s) 197, 344
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 1 cut(s) 29
BsrBI CCGCTC 1 cut(s) 22
BssECI CCNNGG 3 cut(s) 246, 483, 552
BssMI GATC 3 cut(s) 34, 111, 558
BssSI CACGAG 2 cut(s) 146, 284
BssT1I CCWWGG 2 cut(s) 483, 552
Bst2BI CACGAG 2 cut(s) 146, 284
Bst4CI ACNGT 2 cut(s) 407, 423
BstDEI CTNAG 5 cut(s) 153, 257, 392, 441, 564
BstFNI CGCG 2 cut(s) 197, 344
BstH2I RGCGCY 1 cut(s) 254
BstHHI GCGC 2 cut(s) 245, 253
BstKTI GATC 3 cut(s) 37, 114, 561
BstMAI GTCTC 3 cut(s) 95, 342, 526
BstMBI GATC 3 cut(s) 34, 111, 558
BstMCI CGRYCG 3 cut(s) 93, 175, 195
BstSCI CCNGG 2 cut(s) 174, 305
BstSFI CTRYAG 1 cut(s) 234
BstUI CGCG 2 cut(s) 197, 344
BstV1I GCAGC 1 cut(s) 206
BstV2I GAAGAC 1 cut(s) 81
BstX2I RGATCY 1 cut(s) 34
BstYI RGATCY 1 cut(s) 34
BstZI CGGCCG 1 cut(s) 172
Bsu36I CCTNAGG 1 cut(s) 392
BsuRI GGCC 4 cut(s) 98, 140, 174, 305
CfoI GCGC 2 cut(s) 245, 253
Cfr13I GGNCC 2 cut(s) 24, 388
CpoI CGGWCCG 1 cut(s) 24
CseI GACGC 1 cut(s) 186
Csp6I GTAC 1 cut(s) 384
CspI CGGWCCG 1 cut(s) 24
CviJI RGCY 9 cut(s) 98, 140, 145, 152, 174, 205, 268, 305, 418
CviKI_1 RGCY 9 cut(s) 98, 140, 145, 152, 174, 205, 268, 305, 418
CviQI GTAC 1 cut(s) 384
DdeI CTNAG 5 cut(s) 153, 257, 392, 441, 564
DpnI GATC 3 cut(s) 36, 113, 560
DpnII GATC 3 cut(s) 34, 111, 558
EaeI YGGCCR 1 cut(s) 172
EagI CGGCCG 1 cut(s) 172
EciI GGCGGA 1 cut(s) 34
Ecl136II GAGCTC 1 cut(s) 145
EclXI CGGCCG 1 cut(s) 172
Eco130I CCWWGG 2 cut(s) 483, 552
Eco24I GRGCYC 1 cut(s) 147
Eco47I GGWCC 2 cut(s) 24, 388
Eco52I CGGCCG 1 cut(s) 172
Eco53kI GAGCTC 1 cut(s) 145
Eco57I CTGAAG 1 cut(s) 389
Eco81I CCTNAGG 1 cut(s) 392
EcoICRI GAGCTC 1 cut(s) 145
EcoT14I CCWWGG 2 cut(s) 483, 552
EcoT38I GRGCYC 1 cut(s) 147
ErhI CCWWGG 2 cut(s) 483, 552
Esp3I CGTCTC 1 cut(s) 95
FaiI YATR 3 cut(s) 458, 460, 506
FbaI TGATCA 1 cut(s) 111
FblI GTMKAC 1 cut(s) 123
Fnu4HI GCNGC 3 cut(s) 172, 220, 317
FriOI GRGCYC 1 cut(s) 147
Fsp4HI GCNGC 3 cut(s) 172, 220, 317
FspBI CTAG 1 cut(s) 188
GlaI GCGC 2 cut(s) 244, 252
GluI GCNGC 3 cut(s) 172, 220, 317
HaeII RGCGCY 1 cut(s) 254
HaeIII GGCC 4 cut(s) 98, 140, 174, 305
HapII CCGG 3 cut(s) 8, 175, 306
HgaI GACGC 1 cut(s) 186
HhaI GCGC 2 cut(s) 245, 253
Hin6I GCGC 2 cut(s) 243, 251
HinP1I GCGC 2 cut(s) 243, 251
HinfI GANTC 7 cut(s) 29, 86, 106, 165, 230, 335, 466
HpaII CCGG 3 cut(s) 8, 175, 306
HphI GGTGA 1 cut(s) 419
Hpy166II GTNNAC 2 cut(s) 124, 289
Hpy188I TCNGA 6 cut(s) 28, 34, 39, 59, 111, 471
Hpy188III TCNNGA 2 cut(s) 17, 286
Hpy8I GTNNAC 2 cut(s) 124, 289
Hpy99I CGWCG 2 cut(s) 18, 202
HpyAV CCTTC 2 cut(s) 394, 509
HpyCH4III ACNGT 2 cut(s) 407, 423
HpyCH4V TGCA 2 cut(s) 211, 222
HpyF3I CTNAG 5 cut(s) 153, 257, 392, 441, 564
HspAI GCGC 2 cut(s) 243, 251
Ksp22I TGATCA 1 cut(s) 111
Kzo9I GATC 3 cut(s) 34, 111, 558
LmnI GCTCC 2 cut(s) 210, 272
LpnPI CCDG 6 cut(s) 21, 61, 188, 221, 319, 404
Lsp1109I GCAGC 1 cut(s) 206
LweI GCATC 2 cut(s) 55, 220
MaeI CTAG 1 cut(s) 188
MaeIII GTNAC 2 cut(s) 50, 542
MalI GATC 3 cut(s) 36, 113, 560
MbiI CCGCTC 1 cut(s) 22
MboI GATC 3 cut(s) 34, 111, 558
MboII GAAGA 3 cut(s) 86, 382, 500
MflI RGATCY 1 cut(s) 34
MhlI GDGCHC 1 cut(s) 147
MluCI AATT 1 cut(s) 491
MluI ACGCGT 1 cut(s) 342
MlyI GAGTC 5 cut(s) 80, 115, 159, 224, 344
MmeI TCCRAC 3 cut(s) 82, 405, 432
MnlI CCTC 9 cut(s) 65, 88, 137, 256, 295, 352, 387, 403, 436
MroXI GAANNNNTTC 1 cut(s) 476
MslI CAYNNNNRTG 1 cut(s) 329
MspA1I CMGCKG 1 cut(s) 261
MspI CCGG 3 cut(s) 8, 175, 306
MspR9I CCNGG 2 cut(s) 176, 307
MvnI CGCG 2 cut(s) 197, 344
NciI CCSGG 2 cut(s) 176, 307
NdeII GATC 3 cut(s) 34, 111, 558
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 4 cut(s) 124, 147, 166, 227
NmuCI GTSAC 2 cut(s) 50, 542
OliI CACNNNNGTG 1 cut(s) 329
PcsI WCGNNNNNNNCGW 2 cut(s) 90, 297
PdmI GAANNNNTTC 1 cut(s) 476
PfeI GAWTC 2 cut(s) 29, 466
Pfl23II CGTACG 1 cut(s) 383
PflFI GACNNNGTC 2 cut(s) 89, 196
PkrI GCNGC 3 cut(s) 173, 221, 318
PleI GAGTC 5 cut(s) 80, 114, 159, 224, 343
PpsI GAGTC 5 cut(s) 80, 114, 159, 224, 343
Psp124BI GAGCTC 1 cut(s) 147
PspLI CGTACG 1 cut(s) 383
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 2 cut(s) 24, 388
PsuI RGATCY 1 cut(s) 34
PsyI GACNNNGTC 2 cut(s) 89, 196
RsaI GTAC 1 cut(s) 385
RsaNI GTAC 1 cut(s) 384
RseI CAYNNNNRTG 1 cut(s) 329
Rsr2I CGGWCCG 1 cut(s) 24
RsrII CGGWCCG 1 cut(s) 24
SacI GAGCTC 1 cut(s) 147
SatI GCNGC 3 cut(s) 172, 220, 317
Sau3AI GATC 3 cut(s) 34, 111, 558
Sau96I GGNCC 2 cut(s) 24, 388
SchI GAGTC 5 cut(s) 80, 115, 159, 224, 344
ScrFI CCNGG 2 cut(s) 176, 307
SduI GDGCHC 1 cut(s) 147
SetI ASST 9 cut(s) 57, 129, 147, 189, 207, 270, 393, 420, 452
SfaNI GCATC 2 cut(s) 55, 220
SfcI CTRYAG 1 cut(s) 234
SinI GGWCC 2 cut(s) 24, 388
SmiMI CAYNNNNRTG 1 cut(s) 329
Sse9I AATT 1 cut(s) 491
SsiI CCGC 8 cut(s) 22, 45, 171, 195, 261, 317, 329, 349
SspMI CTAG 1 cut(s) 188
SstI GAGCTC 1 cut(s) 147
StyD4I CCNGG 2 cut(s) 174, 305
StyI CCWWGG 2 cut(s) 483, 552
TaaI ACNGT 2 cut(s) 407, 423
TaqI TCGA 5 cut(s) 84, 93, 129, 158, 200
TaqII GACCGA 1 cut(s) 79
TasI AATT 1 cut(s) 491
TauI GCSGC 2 cut(s) 174, 319
TfiI GAWTC 2 cut(s) 29, 466
TseFI GTSAC 2 cut(s) 50, 542
TseI GCWGC 1 cut(s) 219
Tsp45I GTSAC 2 cut(s) 50, 542
TspGWI ACGGA 1 cut(s) 401
Tth111I GACNNNGTC 2 cut(s) 89, 196
VpaK11BI GGWCC 2 cut(s) 24, 388
XapI RAATTY 1 cut(s) 491
XmiI GTMKAC 1 cut(s) 123
XmnI GAANNNNTTC 1 cut(s) 476
XspI CTAG 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.