RchiOBHm_Chr2g0168671

OST-HTH/LOTUS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000679
Physical Location & Seq
Reverse (-)
83069820 .. 83075811
5992 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53630

Sequence Viewer

Length: 3264 bp
ATGGATCTGGAAATACGAAGCTTCGGAGCGTCGGAGCTCTTCAACTCCTCTCCGAAATGTAGCTACTTTTCTTATAATGGCGGTGAAAGCGCCGGTTCGTGTTCCGTCGGTGCCGCGGCGAGTGCGGACCGATTCGACTCGGTCGATGCCGAGACGAGTCTGATCAGAGCCGTCTATCTCGACAGCAAGGCCGAGCTTTTGACTCAGACGAAGCGGCTCGCCGGCGGCGACGTGCTGGACCGAGCGACGGCGTCGAAGCTCCTGCACCTCTGCTGCAACTTCGACTCGGCCGAGTGCGCCTCCGCGCTTCTCGGCGGCGAACTCGGGACAGTTCCGCTCGTGAACGAGTTCGACGACTCGGGAAAATCGCCGCTGCACACGGCGGCGTCGGCTCACGCGTCGCGGTGCGCGGAGGTTTTGCTGAAGAAACACGCTCGTACGGACCTGAGGACCAAGGACGGTCAGGCACAGCTAGCACTGGAGTTGTCGCTTAGTGACACAAGGATGGATCTGAATTGGAATCCGAACGATTTCTCCATCGAAGACTTGGTGATTCACATGAGTGAAAAGGATTTGAGCACGGTTAAACTTCTGAGTGAAAAAACCAAGGAGATTGCTGACGTGGCGTATGGTTACGCCGTTAAAGGCCGTGTAGCTTCTCTGACGGCGCTGCTCATCGTCGCCGCCGAGAAGGTTAACGATTCCGTCTTGGAGTTGCGTGATTCCGAATCGGGGACCAAGGAGAAGTTGACTATCTACGAATGGCTTATAAGGGAGGCTTTGTCCATGGGCGGCCGTACAGCGTTGCCGTTGAGGGCGGCCAAACGCGCCTCCACGGCTACGGAGGGGGAAAACTCGGAGAGGAGGAAGCTCGTGCTTCGAGAGATAGAACTGCTTCAGCTCTTCGGAGCTGTAGCTCAGAGCAGCGGTGGTGGGGACAAGAAGGTGACTTCGCCACTCATCCGTGCAGCTCAGGGAGGAGACGAAGCAGTTATTCAGCTGCTTTTGAAGACAAATATAGATGTCAATGATGCTGATGCAGATGGAAATTCTGCTCTTCATTGGATCCTTAAATTGTCCAGATTGTTGTGTCCCCAACAGATCAAAATTGTATTGCTTCTTATTGATCATGGTGCCCGAGTAAGCCAAAGGAATAGATTGGGGTTAACAGCATTTCATATTGCTGCCGGTAATGGTAATTCAGAGGCACTTGAGGTCCTTCTGTTGGAAGACCCAGTTGGCGTCCAATATAGGACGGAAATGAAAGAAACGCCCCTGTTTTTCGCTGTGAAGAATGAGAGTAAGGAGTGTACTGAGCTTCTTTTAACCTGGGGAGCAAATAGTGAAGTCCTTAATCTGCGGCAAAGAGCTATAGACCTGGCAACCTCTCAAGATATGCGTTACATATTGCTGAACCCAACCACTGTTAGCCTCATTAATCACGCTTTCCCCAATCAACATAAATGTACGGCTTTAGAGTTGACAGATGAAGACACCGCTAATGAAAGAAATGTTAACTCAAGCACCAAGGCAGAGGTTTGTAAGTACTTTGACTCGCATCGAGGATGTGTCAGAGGAGCTAAGTGCTTTTATGCACATTGTGAGGAGGAGTCTCGGAAAGTGAAGGAGGGAGCAGACCGGAGTCATTTTTCTGCTGCTAAACTACTCAAACACAAAATCTTTGTTGGTGGACTTCCTCCATCAGTGGACTCTGATGATTTGGGAAAGTTCTGTGAAGAGGAGTTTGGATCAGTGGACGATGCTATAGTCATTGTAACTCAAACAGAAAACAAAATACAATCTCGAGGCTTTGGTTTTGTCACATTCAGAGAGGAGAAATCTGCTTCAGAAGCTATGCAAGCGCACTACATCACCATGAGAGGCAAGAAAGTTGAAATAAAAAGTTTAATTCCAAAATTGGTTTTGGGAGCTGAGTTTGAGAAGATGTCACCTCAACAACGACAACAAGATAAGAATATTCAAAATCAGCTTCCACAGCAGAAGTCGAACGAGAATATTATGGAAGGTGATAACCCTGAACAAGGTTCTTGGGCTGATAGATTAGTCCTTGGACAACCAGTGGCGTGTTCCACTGAACCTCAAGTTACCAAGAGCTCTGAGGATCCAAGCACTCCTACATGGCTAAAGGTTTTCAAGAAGTGGTTTCCTGGGTTCTTACAAGGTCTGTCAAAGCATTCAAATTATGCTCTGTCTTCTCTAAAAGCTGACTTCAGGGCTAAATTTGGGTTAGAACTGGATCACTCATCTGTGGGCTACTCTAAGCTCAGTGACTTTGTGAAATGTTTCTCTGATTTATGTACTGTTGAGATTCTCCCCATCTGCAAACGTGGAACTCCAACTCACGTGATCCTCCGGCCAAAATTTTCTAGGCCTCAACGCCGACTCCTTCCTACACTGAGAACACCTCACACTCTGTCTCTGTCTGCACTGCTTGTCAAAGGCGGTGATACAAAGTGTCTTTTGGACCTCTCAAGTGGCGATTGTGGTGATTCAAAGTGTCTTCAGGACCATTCGAAGTGTTTTCAGGACCTTTCAGTTAACAATGGCAGTGACTCAAAGTGTTTTCAGAACCTTACAATTGAAAGCATTAGTGATACAAAGTGTCTTCAGGACATTTCAGCTGACAATGCTGCTGATTCAAAGTGCCTTCAGGACCTTTCACTTGACTTTAGTGGTGATTCAAACTGTATTGAGGACCTTTCCATTTGCAATGCTGGTGAGAAGTTTGGCTCAAGTGTCACTAGCCATGGGCAAGAGAAACCCTCCTCTGGGTATCCTGAAATGAACTCTGCAGAGGATAAAGGACAATGTGTGCACCTGAGGTTCCTTCAATTCTTAAAACCAGATCCTCTATTCTATGGTCAGAAAGAAACTGACGTGCTTGCCGGAGAGACTGACAATGAAAAGGGACAGTGCAGTGCGGGGGATAAAGGAAGTATAAATATTAGGGATCCAAGACGGCATCTCGTTTTGGAGTCCCTGTCCAAGAAATGGAGCAAATCCTACTTCCTTCGTGAATTTGATTTCTACAAGATTTACAAGGAAAGATTATCGGTAAGAAGGTGTTTTGCATGCAACCAGCAGAAGATGCTATGGGCCAATTTCCCTTGTAAACACTTGCTGTGGTGCACTGACTGTAAGTTGCGGGCAATAGAGGCTTCTGGTCTTTTCCCACACAAATGCGTGCTGTGTGACACAGAAGTACAGAAAATGGATTTAGTACTTCCATTTACTAGTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 OST-HTH RRM_1

Protein Analysis

1087

Amino Acids

120.01

Weight (kDa)

6.09

Isoelectric Point (pI)

42.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 75 - 148 2.6e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 315 - 384 8.9e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 368 - 447 1.5e-10 Ankyrin repeats (3 copies)
Ank_4 PF13637 368 - 408 3.8e-06 Ankyrin repeats (many copies)
RRM_1 PF00076 560 - 631 9.8e-12 RNA recognition motif
OST-HTH PF12872 714 - 776 7.7e-08 OST-HTH/LOTUS domain
zf-C3HC4_3 PF13920 1027 - 1078 9.4e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 75, 770
AccB1I GGYRCC 2 cut(s) 110, 1133
AccB7I CCANNNNNTGG 1 cut(s) 3012
AccBSI CCGCTC 1 cut(s) 337
AccII CGCG 6 cut(s) 116, 305, 398, 403, 410, 828
AcoI YGGCCR 4 cut(s) 288, 793, 819, 2375
AcsI RAATTY 4 cut(s) 1048, 2240, 2381, 3038
AcuI CTGAAG 7 cut(s) 443, 881, 1830, 2215, 2507, 2612, 2654
AcvI CACGTG 1 cut(s) 2365
AcyI GRCGYC 3 cut(s) 251, 386, 1242
AdeI CACNNNGTG 1 cut(s) 1601
AfaI GTAC 8 cut(s) 439, 799, 1312, 1468, 1547, 2320, 3225, 3243
AfiI CCNNNNNNNGG 7 cut(s) 247, 732, 1225, 2042, 2789, 2790, 3012
AflIII ACRYGT 1 cut(s) 396
AhlI ACTAGT 1 cut(s) 3254
AjiI CACGTC 3 cut(s) 232, 622, 2899
AjnI CCWGG 3 cut(s) 1328, 1377, 2167
AjuI GAANNNNNNNTTGG 4 cut(s) 1221, 1253, 1728, 1760
AleI CACNNNNGTG 1 cut(s) 561
AloI GAACNNNNNNTCC 2 cut(s) 518, 550
Alw21I GWGCWC 5 cut(s) 39, 581, 2117, 2838, 3152
Alw26I GTCTC 5 cut(s) 146, 975, 1617, 2442, 2906
Alw44I GTGCAC 2 cut(s) 2834, 3148
Ama87I CYCGRG 4 cut(s) 323, 358, 1137, 1803
AoxI GGCC 8 cut(s) 189, 288, 646, 793, 819, 2375, 2390, 3117
ApaLI GTGCAC 2 cut(s) 2834, 3148
ApeKI GCWGC 9 cut(s) 273, 373, 670, 924, 968, 1000, 1184, 1655, 2651
ApoI RAATTY 4 cut(s) 1048, 2240, 2381, 3038
AseI ATTAAT 1 cut(s) 1437
Asp700I GAANNNNTTC 4 cut(s) 347, 530, 894, 2303
AspLEI GCGC 7 cut(s) 92, 299, 307, 410, 670, 830, 1864
AsuII TTCGAA 1 cut(s) 2534
AsuNHI GCTAGC 1 cut(s) 472
AvaI CYCGRG 4 cut(s) 323, 358, 1137, 1803
AxyI CCTNAGG 2 cut(s) 446, 2840
BaeGI GKGCMC 3 cut(s) 1138, 2838, 3152
BamHI GGATCC 3 cut(s) 1065, 2122, 2971
BanI GGYRCC 2 cut(s) 110, 1133
BanII GRGCYC 2 cut(s) 39, 2117
BauI CACGAG 2 cut(s) 338, 872
BbrPI CACGTG 1 cut(s) 2365
BbsI GAAGAC 7 cut(s) 549, 1016, 1236, 1497, 2205, 2513, 2618
Bbv12I GWGCWC 5 cut(s) 39, 581, 2117, 2838, 3152
BbvI GCAGC 9 cut(s) 260, 360, 657, 936, 980, 987, 1171, 1642, 2638
BccI CCATC 5 cut(s) 499, 545, 1037, 1708, 2345
BcgI CGANNNNNNTGC 2 cut(s) 244, 278
BciT130I CCWGG 3 cut(s) 1330, 1379, 2169
BciVI GTATCC 1 cut(s) 2805
BclI TGATCA 2 cut(s) 162, 1126
BcoDI GTCTC 5 cut(s) 146, 975, 1617, 2442, 2906
BcuI ACTAGT 1 cut(s) 3254
BfaI CTAG 4 cut(s) 473, 2388, 2763, 3255
BfmI CTRYAG 4 cut(s) 912, 1371, 1764, 2811
BfoI RGCGCY 2 cut(s) 93, 671
BfuI GTATCC 1 cut(s) 2805
BglI GCCNNNNNGGC 1 cut(s) 836
BmcAI AGTACT 2 cut(s) 1547, 3243
Bme1390I CCNGG 3 cut(s) 1330, 1379, 2169
BmeT110I CYCGRG 4 cut(s) 323, 358, 1137, 1803
BmgBI CACGTC 3 cut(s) 232, 622, 2899
BmiI GGNNCC 7 cut(s) 112, 736, 1067, 1135, 2124, 2846, 2973
BmrFI CCNGG 3 cut(s) 1330, 1379, 2169
BmrI ACTGGG 1 cut(s) 1229
BmsI GCATC 7 cut(s) 136, 1021, 1027, 1567, 1750, 2992, 3099
BmtI GCTAGC 1 cut(s) 476
BmuI ACTGGG 1 cut(s) 1229
BoxI GACNNNNGTC 1 cut(s) 1641
BpiI GAAGAC 7 cut(s) 549, 1016, 1236, 1497, 2205, 2513, 2618
BplI GAGNNNNNCTC 6 cut(s) 284, 316, 2410, 2442, 2768, 2800
BpmI CTGGAG 1 cut(s) 500
Bpu10I CCTNAGC 1 cut(s) 972
Bpu14I TTCGAA 1 cut(s) 2534
BpuEI CTTGAG 6 cut(s) 1232, 1374, 1504, 2085, 2476, 2737
BsaAI YACGTR 1 cut(s) 2365
BsaHI GRCGYC 3 cut(s) 251, 386, 1242
BsaWI WCCGGW 1 cut(s) 1638
BsaXI ACNNNNNCTCC 8 cut(s) 518, 548, 767, 797, 2388, 2418, 2987, 3017
Bsc4I CCNNNNNNNGG 7 cut(s) 247, 732, 1225, 2042, 2789, 2790, 3012
Bse118I RCCGGY 3 cut(s) 92, 221, 1187
Bse1I ACTGG 4 cut(s) 483, 1235, 2078, 2259
Bse21I CCTNAGG 2 cut(s) 446, 2840
Bse3DI GCAATG 1 cut(s) 2737
BseBI CCWGG 3 cut(s) 1330, 1379, 2169
BseGI GGATG 3 cut(s) 510, 960, 1571
BseLI CCNNNNNNNGG 7 cut(s) 247, 732, 1225, 2042, 2789, 2790, 3012
BseMI GCAATG 1 cut(s) 2737
BseNI ACTGG 4 cut(s) 483, 1235, 2078, 2259
BseRI GAGGAG 9 cut(s) 37, 877, 993, 1590, 1619, 1622, 1754, 1847, 2776
BseSI GKGCMC 3 cut(s) 1138, 2838, 3152
BseX3I CGGCCG 2 cut(s) 288, 793
BseXI GCAGC 9 cut(s) 260, 360, 657, 936, 980, 987, 1171, 1642, 2638
BsgI GTGCAG 5 cut(s) 248, 359, 987, 2430, 2956
Bsh1236I CGCG 6 cut(s) 116, 305, 398, 403, 410, 828
Bsh1285I CGRYCG 3 cut(s) 144, 291, 796
BshFI GGCC 8 cut(s) 191, 290, 648, 795, 821, 2377, 2392, 3119
BshNI GGYRCC 2 cut(s) 110, 1133
BsiEI CGRYCG 3 cut(s) 144, 291, 796
BsiHKAI GWGCWC 5 cut(s) 39, 581, 2117, 2838, 3152
BsiHKCI CYCGRG 4 cut(s) 323, 358, 1137, 1803
BsiSI CCGG 6 cut(s) 93, 222, 1188, 1639, 2374, 2907
BsiWI CGTACG 1 cut(s) 437
BslFI GGGAC 6 cut(s) 340, 748, 950, 1077, 2943, 2983
BslI CCNNNNNNNGG 7 cut(s) 247, 732, 1225, 2042, 2789, 2790, 3012
BsmAI GTCTC 5 cut(s) 146, 975, 1617, 2442, 2906
BsmBI CGTCTC 2 cut(s) 146, 975
BsmFI GGGAC 6 cut(s) 340, 748, 950, 1077, 2943, 2983
BsmI GAATGC 1 cut(s) 2194
BsnI GGCC 8 cut(s) 191, 290, 648, 795, 821, 2377, 2392, 3119
BsoBI CYCGRG 4 cut(s) 323, 358, 1137, 1803
Bsp119I TTCGAA 1 cut(s) 2534
Bsp1286I GDGCHC 6 cut(s) 39, 581, 1138, 2117, 2838, 3152
Bsp19I CCATGG 2 cut(s) 786, 2767
BspANI GGCC 8 cut(s) 191, 290, 648, 795, 821, 2377, 2392, 3119
BspFNI CGCG 6 cut(s) 116, 305, 398, 403, 410, 828
BspLI GGNNCC 7 cut(s) 112, 736, 1067, 1135, 2124, 2846, 2973
BspMAI CTGCAG 1 cut(s) 2815
BspOI GCTAGC 1 cut(s) 476
BspQI GCTCTTC 3 cut(s) 44, 908, 1062
BspT104I TTCGAA 1 cut(s) 2534
BspT107I GGYRCC 2 cut(s) 110, 1133
BsrBI CCGCTC 1 cut(s) 337
BsrDI GCAATG 1 cut(s) 2737
BsrFI RCCGGY 3 cut(s) 92, 221, 1187
BsrI ACTGG 4 cut(s) 483, 1235, 2078, 2259
BssAI RCCGGY 3 cut(s) 92, 221, 1187
BssNI GRCGYC 3 cut(s) 251, 386, 1242
BssSI CACGAG 2 cut(s) 338, 872
BssT1I CCWWGG 7 cut(s) 453, 606, 738, 786, 1527, 2068, 2767
Bst2BI CACGAG 2 cut(s) 338, 872
Bst2UI CCWGG 3 cut(s) 1330, 1379, 2169
Bst4CI ACNGT 8 cut(s) 331, 461, 583, 1426, 2323, 2708, 2934, 3158
Bst6I CTCTTC 4 cut(s) 44, 908, 1062, 1731
BstACI GRCGYC 3 cut(s) 251, 386, 1242
BstAPI GCANNNNNTGC 1 cut(s) 3109
BstBAI YACGTR 1 cut(s) 2365
BstBI TTCGAA 1 cut(s) 2534
BstC8I GCNNGC 8 cut(s) 219, 223, 474, 1860, 2904, 3094, 3168, 3206
BstDSI CCRYGG 4 cut(s) 114, 786, 834, 2767
BstENI CCTNNNNNAGG 1 cut(s) 2040
BstF5I GGATG 3 cut(s) 510, 960, 1571
BstFNI CGCG 6 cut(s) 116, 305, 398, 403, 410, 828
BstH2I RGCGCY 2 cut(s) 93, 671
BstHHI GCGC 7 cut(s) 92, 299, 307, 410, 670, 830, 1864
BstMAI GTCTC 5 cut(s) 146, 975, 1617, 2442, 2906
BstMCI CGRYCG 3 cut(s) 144, 291, 796
BstNI CCWGG 3 cut(s) 1330, 1379, 2169
BstNSI RCATGY 1 cut(s) 3096
BstPAI GACNNNNGTC 1 cut(s) 1641
BstSCI CCNGG 3 cut(s) 1328, 1377, 2167
BstSFI CTRYAG 4 cut(s) 912, 1371, 1764, 2811
BstSLI GKGCMC 3 cut(s) 1138, 2838, 3152
BstUI CGCG 6 cut(s) 116, 305, 398, 403, 410, 828
BstV1I GCAGC 9 cut(s) 260, 360, 657, 936, 980, 987, 1171, 1642, 2638
BstV2I GAAGAC 7 cut(s) 549, 1016, 1236, 1497, 2205, 2513, 2618
BstX2I RGATCY 6 cut(s) 4, 508, 1065, 2122, 2866, 2971
BstYI RGATCY 6 cut(s) 4, 508, 1065, 2122, 2866, 2971
BstZI CGGCCG 2 cut(s) 288, 793
Bsu36I CCTNAGG 2 cut(s) 446, 2840
BsuI GTATCC 1 cut(s) 2805
BsuRI GGCC 8 cut(s) 191, 290, 648, 795, 821, 2377, 2392, 3119
BtgI CCRYGG 4 cut(s) 114, 786, 834, 2767
BtrI CACGTC 3 cut(s) 232, 622, 2899
BtsCI GGATG 3 cut(s) 510, 960, 1571
BtsI GCAGTG 3 cut(s) 2447, 2575, 2944
Cac8I GCNNGC 8 cut(s) 219, 223, 474, 1860, 2904, 3094, 3168, 3206
CfoI GCGC 7 cut(s) 92, 299, 307, 410, 670, 830, 1864
Cfr10I RCCGGY 3 cut(s) 92, 221, 1187
Cfr42I CCGCGG 1 cut(s) 117
CpoI CGGWCCG 1 cut(s) 127
CseI GACGC 5 cut(s) 18, 240, 375, 387, 1231
Csp6I GTAC 8 cut(s) 438, 798, 1311, 1467, 1546, 2319, 3224, 3242
CspI CGGWCCG 1 cut(s) 127
CviAII CATG 7 cut(s) 559, 787, 1130, 1876, 2139, 2768, 3093
CviQI GTAC 8 cut(s) 438, 798, 1311, 1467, 1546, 2319, 3224, 3242
DraIII CACNNNGTG 1 cut(s) 1601
EaeI YGGCCR 4 cut(s) 288, 793, 819, 2375
EagI CGGCCG 2 cut(s) 288, 793
Eam1104I CTCTTC 4 cut(s) 44, 908, 1062, 1731
EarI CTCTTC 4 cut(s) 44, 908, 1062, 1731
Ecl136II GAGCTC 2 cut(s) 37, 2115
EclXI CGGCCG 2 cut(s) 288, 793
Eco130I CCWWGG 7 cut(s) 453, 606, 738, 786, 1527, 2068, 2767
Eco147I AGGCCT 1 cut(s) 2392
Eco24I GRGCYC 2 cut(s) 39, 2117
Eco52I CGGCCG 2 cut(s) 288, 793
Eco53kI GAGCTC 2 cut(s) 37, 2115
Eco57I CTGAAG 7 cut(s) 443, 881, 1830, 2215, 2507, 2612, 2654
Eco72I CACGTG 1 cut(s) 2365
Eco81I CCTNAGG 2 cut(s) 446, 2840
Eco88I CYCGRG 4 cut(s) 323, 358, 1137, 1803
EcoICRI GAGCTC 2 cut(s) 37, 2115
EcoNI CCTNNNNNAGG 1 cut(s) 2040
EcoO109I RGGNCCY 4 cut(s) 1216, 2548, 2674, 2716
EcoRII CCWGG 3 cut(s) 1328, 1377, 2167
EcoT14I CCWWGG 7 cut(s) 453, 606, 738, 786, 1527, 2068, 2767
EcoT38I GRGCYC 2 cut(s) 39, 2117
ErhI CCWWGG 7 cut(s) 453, 606, 738, 786, 1527, 2068, 2767
Esp3I CGTCTC 2 cut(s) 146, 975
FaeI CATG 7 cut(s) 562, 790, 1133, 1879, 2142, 2771, 3096
FalI AAGNNNNNCTT 2 cut(s) 763, 795
FaqI GGGAC 6 cut(s) 340, 748, 950, 1077, 2943, 2983
FatI CATG 7 cut(s) 558, 786, 1129, 1875, 2138, 2767, 3092
FauI CCCGC 2 cut(s) 2935, 3159
FbaI TGATCA 2 cut(s) 162, 1126
FokI GGATG 3 cut(s) 517, 947, 1578
FriOI GRGCYC 2 cut(s) 39, 2117
FspBI CTAG 4 cut(s) 473, 2388, 2763, 3255
GlaI GCGC 7 cut(s) 91, 298, 306, 409, 669, 829, 1863
GsuI CTGGAG 1 cut(s) 500
HaeII RGCGCY 2 cut(s) 93, 671
HaeIII GGCC 8 cut(s) 191, 290, 648, 795, 821, 2377, 2392, 3119
HapII CCGG 6 cut(s) 93, 222, 1188, 1639, 2374, 2907
HgaI GACGC 5 cut(s) 18, 240, 375, 387, 1231
HhaI GCGC 7 cut(s) 92, 299, 307, 410, 670, 830, 1864
Hin1I GRCGYC 3 cut(s) 251, 386, 1242
Hin1II CATG 7 cut(s) 562, 790, 1133, 1879, 2142, 2771, 3096
Hin6I GCGC 7 cut(s) 90, 297, 305, 408, 668, 828, 1862
HinP1I GCGC 7 cut(s) 90, 297, 305, 408, 668, 828, 1862
HincII GTYRAC 6 cut(s) 697, 750, 1167, 1482, 1516, 2560
HindII GTYRAC 6 cut(s) 697, 750, 1167, 1482, 1516, 2560
HindIII AAGCTT 1 cut(s) 19
HpaI GTTAAC 4 cut(s) 697, 1167, 1516, 2560
HpaII CCGG 6 cut(s) 93, 222, 1188, 1639, 2374, 2907
Hpy99I CGWCG 9 cut(s) 34, 110, 233, 250, 256, 356, 391, 403, 683
HpyCH4III ACNGT 8 cut(s) 331, 461, 583, 1426, 2323, 2708, 2934, 3158
HpyCH4IV ACGT 5 cut(s) 231, 621, 2347, 2364, 2898
HpySE526I ACGT 5 cut(s) 231, 621, 2347, 2364, 2898
Hsp92I GRCGYC 3 cut(s) 251, 386, 1242
Hsp92II CATG 7 cut(s) 562, 790, 1133, 1879, 2142, 2771, 3096
HspAI GCGC 7 cut(s) 90, 297, 305, 408, 668, 828, 1862
KroI GCCGGC 1 cut(s) 221
KroNI GCCGGC 1 cut(s) 223
Ksp22I TGATCA 2 cut(s) 162, 1126
KspAI GTTAAC 4 cut(s) 697, 1167, 1516, 2560
KspI CCGCGG 1 cut(s) 117
LguI GCTCTTC 3 cut(s) 44, 908, 1062
LmnI GCTCC 9 cut(s) 26, 34, 264, 908, 1334, 1577, 1631, 1928, 3015
Lsp1109I GCAGC 9 cut(s) 260, 360, 657, 936, 980, 987, 1171, 1642, 2638
LweI GCATC 7 cut(s) 136, 1021, 1027, 1567, 1750, 2992, 3099
MaeI CTAG 4 cut(s) 473, 2388, 2763, 3255
MaeII ACGT 5 cut(s) 231, 621, 2347, 2364, 2898
MbiI CCGCTC 1 cut(s) 337
MfeI CAATTG 1 cut(s) 2598
MflI RGATCY 6 cut(s) 4, 508, 1065, 2122, 2866, 2971
MhlI GDGCHC 6 cut(s) 39, 581, 1138, 2117, 2838, 3152
MluI ACGCGT 1 cut(s) 396
MmeI TCCRAC 3 cut(s) 12, 1206, 2381
MreI CGCCGGCG 1 cut(s) 221
MroNI GCCGGC 1 cut(s) 221
MroXI GAANNNNTTC 4 cut(s) 347, 530, 894, 2303
MslI CAYNNNNRTG 4 cut(s) 503, 561, 1874, 3199
MspA1I CMGCKG 5 cut(s) 116, 373, 927, 1000, 2642
MspI CCGG 6 cut(s) 93, 222, 1188, 1639, 2374, 2907
MspR9I CCNGG 3 cut(s) 1330, 1379, 2169
MunI CAATTG 1 cut(s) 2598
Mva1269I GAATGC 1 cut(s) 2194
MvaI CCWGG 3 cut(s) 1330, 1379, 2169
MvnI CGCG 6 cut(s) 116, 305, 398, 403, 410, 828
NaeI GCCGGC 1 cut(s) 223
NcoI CCATGG 2 cut(s) 786, 2767
NgoMIV GCCGGC 1 cut(s) 221
NheI GCTAGC 1 cut(s) 472
NlaIII CATG 7 cut(s) 562, 790, 1133, 1879, 2142, 2771, 3096
NlaIV GGNNCC 7 cut(s) 112, 736, 1067, 1135, 2124, 2846, 2973
NmeAIII GCCGAG 6 cut(s) 175, 217, 266, 291, 316, 712
NmuCI GTSAC 8 cut(s) 494, 946, 1819, 1947, 2288, 2570, 2758, 3212
NspI RCATGY 1 cut(s) 3096
NspV TTCGAA 1 cut(s) 2534
OliI CACNNNNGTG 1 cut(s) 561
PaeI GCATGC 1 cut(s) 3096
PaeR7I CTCGAG 1 cut(s) 1803
PceI AGGCCT 1 cut(s) 2392
PciSI GCTCTTC 3 cut(s) 44, 908, 1062
PcsI WCGNNNNNNNCGW 6 cut(s) 141, 225, 288, 351, 395, 684
PctI GAATGC 1 cut(s) 2194
PdiI GCCGGC 1 cut(s) 223
PdmI GAANNNNTTC 4 cut(s) 347, 530, 894, 2303
Pfl23II CGTACG 1 cut(s) 437
PflFI GACNNNGTC 2 cut(s) 140, 250
PflMI CCANNNNNTGG 1 cut(s) 3012
PmaCI CACGTG 1 cut(s) 2365
PmlI CACGTG 1 cut(s) 2365
Ppu21I YACGTR 1 cut(s) 2365
PpuMI RGGWCCY 4 cut(s) 1216, 2548, 2674, 2716
PshAI GACNNNNGTC 1 cut(s) 1641
PshBI ATTAAT 1 cut(s) 1437
PsiI TTATAA 2 cut(s) 75, 770
Psp124BI GAGCTC 2 cut(s) 39, 2117
Psp5II RGGWCCY 4 cut(s) 1216, 2548, 2674, 2716
Psp6I CCWGG 3 cut(s) 1328, 1377, 2167
PspCI CACGTG 1 cut(s) 2365
PspGI CCWGG 3 cut(s) 1328, 1377, 2167
PspLI CGTACG 1 cut(s) 437
PspN4I GGNNCC 7 cut(s) 112, 736, 1067, 1135, 2124, 2846, 2973
PspPPI RGGWCCY 4 cut(s) 1216, 2548, 2674, 2716
PstI CTGCAG 1 cut(s) 2815
PsuI RGATCY 6 cut(s) 4, 508, 1065, 2122, 2866, 2971
PsyI GACNNNGTC 2 cut(s) 140, 250
PvuII CAGCTG 2 cut(s) 1000, 2642
RsaI GTAC 8 cut(s) 439, 799, 1312, 1468, 1547, 2320, 3225, 3243
RsaNI GTAC 8 cut(s) 438, 798, 1311, 1467, 1546, 2319, 3224, 3242
RseI CAYNNNNRTG 4 cut(s) 503, 561, 1874, 3199
Rsr2I CGGWCCG 1 cut(s) 127
RsrII CGGWCCG 1 cut(s) 127
SacI GAGCTC 2 cut(s) 39, 2117
SacII CCGCGG 1 cut(s) 117
SapI GCTCTTC 3 cut(s) 44, 908, 1062
ScaI AGTACT 2 cut(s) 1547, 3243
ScrFI CCNGG 3 cut(s) 1330, 1379, 2169
SduI GDGCHC 6 cut(s) 39, 581, 1138, 2117, 2838, 3152
SfaNI GCATC 7 cut(s) 136, 1021, 1027, 1567, 1750, 2992, 3099
SfcI CTRYAG 4 cut(s) 912, 1371, 1764, 2811
Sfr274I CTCGAG 1 cut(s) 1803
Sfr303I CCGCGG 1 cut(s) 117
SfuI TTCGAA 1 cut(s) 2534
SgrAI CRCCGGYG 1 cut(s) 221
SgrBI CCGCGG 1 cut(s) 117
SlaI CTCGAG 1 cut(s) 1803
SmiMI CAYNNNNRTG 4 cut(s) 503, 561, 1874, 3199
SmlI CTYRAG 7 cut(s) 1211, 1389, 1519, 1803, 2100, 2491, 2752
SmoI CTYRAG 7 cut(s) 1211, 1389, 1519, 1803, 2100, 2491, 2752
SpeI ACTAGT 1 cut(s) 3254
SphI GCATGC 1 cut(s) 3096
SseBI AGGCCT 1 cut(s) 2392
SspI AATATT 3 cut(s) 1978, 2017, 2965
SspMI CTAG 4 cut(s) 473, 2388, 2763, 3255
SstI GAGCTC 2 cut(s) 39, 2117
StuI AGGCCT 1 cut(s) 2392
StyD4I CCNGG 3 cut(s) 1328, 1377, 2167
StyI CCWWGG 7 cut(s) 453, 606, 738, 786, 1527, 2068, 2767
TaaI ACNGT 8 cut(s) 331, 461, 583, 1426, 2323, 2708, 2934, 3158
TaiI ACGT 5 cut(s) 234, 624, 2350, 2367, 2901
TaqII GACCGA 3 cut(s) 130, 144, 255
TatI WGTACW 5 cut(s) 1310, 1545, 2318, 3223, 3241
TseFI GTSAC 8 cut(s) 494, 946, 1819, 1947, 2288, 2570, 2758, 3212
TseI GCWGC 9 cut(s) 273, 373, 670, 924, 968, 1000, 1184, 1655, 2651
Tsp45I GTSAC 8 cut(s) 494, 946, 1819, 1947, 2288, 2570, 2758, 3212
TspDTI ATGAA 7 cut(s) 1049, 1166, 1277, 1503, 1518, 2819, 2937
TspGWI ACGGA 6 cut(s) 94, 455, 694, 857, 953, 1271
Tth111I GACNNNGTC 2 cut(s) 140, 250
Van91I CCANNNNNTGG 1 cut(s) 3012
VneI GTGCAC 2 cut(s) 2834, 3148
VspI ATTAAT 1 cut(s) 1437
XagI CCTNNNNNAGG 1 cut(s) 2040
XapI RAATTY 4 cut(s) 1048, 2240, 2381, 3038
XceI RCATGY 1 cut(s) 3096
XcmI CCANNNNNNNNNTGG 1 cut(s) 544
XhoI CTCGAG 1 cut(s) 1803
XmnI GAANNNNTTC 4 cut(s) 347, 530, 894, 2303
XspI CTAG 4 cut(s) 473, 2388, 2763, 3255
ZrmI AGTACT 2 cut(s) 1547, 3243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.