MD09G1050300.v1.1

OST-HTH/LOTUS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000679
Physical Location & Seq
Forward (+)
3340082 .. 3347043
6962 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1050300.v1.1.491

Sequence Viewer

Length: 3321 bp
ATGAATCTGGAAATACAAAATTTTGGAGCGACGGAGCTCCTGACCAACTCGCTTCCAAAATGCACCTATTTCTCTTACAATCACGACGAAAACGTCGCGTCCAAGGCCGGCGACGATCTCCGGAGTGCAAAGAGCGAACGGTTCGACTTCGTGGAAGCCGAATCGCTCCTGATCAACTCGGTTTTCCGAGACAACAAGGCACAACTCGTGACTCACGCGAAGCGGTTCGCGGAGGCGTCTGGGGAGGCGATTGACCGGAGCACGGTGTCGAAGCTCCTCCACCTCTGCTGCAACTTCGACTCACCGGAGTGCGCGTCGGCATTGCTAGCCGGAGAGGTCGGAGAGACACCGCTGATAAACGAGCTCGACGATTCCGGGAAATCGGCGCTGCACACGGCGGCTCTGGCTCACGCGGCGAGGTGCGTGGAGGTTTTGCTAAAGAAACACGCTCGTACGGGCCTGAGGACACGAGACGGACGCGCACAGCTTGCTCTGGACCTGTCTCTCTCTAACAGCAGGATCGATGTGATTTGGACTCCGGATGATTACACCATTGAGGACTTGGTTGTTGTTCTTGGTGAAAAGGATTTGACGACTGTGAGACTTCTCAGTGAGAAGACGAAGGAACTAGGTGACGTGGCATATGCAAACGCCGTTGAAGGCCGTATAGTTGCTTTAGCGGCTCTGCTTACAGTAGCTGCGGAGAAGGTGAACGAAGCGGTGTTGGAGTTGCGCGATGCCGATTCGGGTTCCAAAGAGAAGGTGACAATATACGAGTGCGTTATACGGGAAGCTCTGTCCCTGGTCCCTTCAAATACGCCGTTGAGGGCGGCCAAACGCACCTCGAGCGCCACGGAGAGCGACGATGCCGAGAAAAGGAGGATTCTGCTTCACGAGATAGAGCTGCTTCAGCTCTTCGGAGCTGTTGCTCTCAGCAGTGGTACGGATAAGAAGGTGATGTCACCACTCATCCGAGCAGCCCAGGTAGGAGATGAAGCAGTCATTCGTCTGCTTCTGAAGACAAATATAGATGTAAATGATGCTGATGATGCAGAAGGAAATTCTGCTCTTCATTGGACCCTCAAACTGTCGAGATTGTTGCATCCACAGCAGATCAAAATTTTATGGCTTCTCATTAAGCATGGGGCACGAGTGAGCCAGAGGAACAAATTAGGATTAACTTCCTTTCATATTGCTGCTGGTAGTGGTAACACAGAGGCACTTCAGGTCCTTCTGTTGGAAGACCCAGAAGGTGTCCAGTATAAGACAGAAATTAAAGAAACCCCACTGTTCTTTGCTGTGAGAAATGACAACATGGAGTGCGCTGAACTTCTTTTGAGCTGGGGAGCAAATAGTGAAATTCTCAATCTGCGACAAAGACCTATTGACCTGACAAACTCGCAAGATATGCGTTTCATGTTGCTGAACCCGACCAGTGTTAGCCTCAACACCAATCAACATAAATGCCATGCTTGTTCACGAGTCAATGAGGCTTTCTCAAGCACCTGCGAAGCACTTCTAACCATGCAATATGAAGACACCGCCACTGAAAGTAAAGCCGAGATTTGCAAATACTTTGACTCCTCTCGCGGATGTGTCAGAGGATCTAAGTGCTTTTTTTCACATGATGTGGAGGAGCATCGGAAAGTGAAGCATGAAGCAGCCCATAATCATTCTCCTGTTGATAAAGCATTAGCCCTCAAGCGCATCTTCGTAGGAGGACTTCCTCCTTCAGTGGACTCTGATTCGTTGGGTAAGTTTTTTGAAAAAGAATTTGGGCCGGTGGAAGATGCCATAGTCATTTTAGCTCAAATGGAAAACAAGACACACTCTCGAGGCTTTGGATTTGTCACATTTAAAGAGGAGAAATCTGTTTCTGCAGCTGTTCATGCACACTATATCACCATGGCAGGCAAGCCGGTTGAAATAAAAAGCCTAATACCAAAATTGGCAGCTGAGTCGGTGAAAATGGCACTTCGACATCAAGAACAAAAGAATTGCAATTGCCAATCTCCACTTCCAGCAAAGGTGTTGTCCAACGAGATGATTATGGAAGCAAATAAACCCAAACAAGGTTCTTGGCTTGATAAGGTAGTCTACGGCCAACCAAACACATCCCCTCAAGCACATAATACCAGCAGCCCCAAGGATAAAAGCGTGCCTGTATGGCTAAAGGCTTTCAAGAAGTGGTTTCCTCGCTTCTTACAACGTCTGCCAAAGCCCACGGTTGGAGAATATGCTCTCTCCTCTCTGAAAAGTGATTTCAGGGCTTCTGTTGGCTTAGAACTGGATCATTCGCACATTGGCTTCTCTAAGCTCAGTGACTTCATCAAATCTTTCTCCAACTTATGTACCATTAAGCATGTCCCTATTGGAAAACTCGGATCTCTCAGTCACATGGTCCTCCTACCAAATCCCAGGCGTCACCACAACCTTTGTCATGCATTCAAAATGCCTTGCAGTTCCCCTTTTTCTACACCAACTGATGATGGTGGGGATTCAAAATGTCTTCATGACATTTCAGTTGAGGCTGGCGGTGATTCAAAGTGTCTTCAAGACCTCCCAATGGATGATGTTGGTGATTCAAAGTGTCTTCAAGACCTCCCAACGGATGATGTTGGAGATTCAAAGTGCCTTCAGGATATTCCAACCGATGATGTTGGTGATCCAAAGTGTGTTCGGGACATTCCAACTAATGATGGCGGTGATTCAAAGCATCTTCTGGAGCTTTCGGTTGATGATCGTGCCGGAAACTCTGGGTACCCTAAAGAGAGACCCTCCTCTGGGAACCCTGAAGTAACCTCAGGAAAGGATACAACATCCCATTGGGAGCATTCAAGGGTCCTGCAATTCTTGAAGCCAGATCCTGTATTCCATGGTCGAAAAGATTATGATGTGCAAGAAGTAAGAGGACAGCTTGTAGAGAATACAGGAAGCACTAAGCCTAGCGATCCACAGCGGCATTTGGTTTTGGAGGCCCTTACCAGAAATAGGAACAAATCCTCGGGGTTCTTCCTTCGTGAATTTGATTTCTACAAGAACTACAAGGAAAGCATCTTGGAGGGAAAGTGTTTTGCCTGCAACCAGAACCAGATGTCATGGGCCAACTTCCCCTGCCAACACTTGCTGTGGTGCGGCGACTGCAAAGTGCAGGCCATACTGGCATCTAGTGGTTCTGTGCATAAATGCGTGGTTTGTGATGCGCAAGTACACAAACTCATTTCACTTCCGCGGAATGATGAGTTCTTGCCTATGCCACCTGGTCTTTTCCGAACTCCATTCGTGCAGAAGACATCTCCACTGATGACCGGTAACAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 OST-HTH RRM_1

Protein Analysis

1107

Amino Acids

122.28

Weight (kDa)

6.33

Isoelectric Point (pI)

40.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 91 - 151 1e-05 Ankyrin repeats (3 copies)
Ank_2 PF12796 314 - 388 4e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 373 - 451 9.6e-10 Ankyrin repeats (3 copies)
RRM_1 PF00076 570 - 636 1.8e-11 RNA recognition motif
OST-HTH PF12872 723 - 793 6.5e-10 OST-HTH/LOTUS domain
zf-C3HC4_3 PF13920 1022 - 1072 2.1e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1514
AasI GACNNNNNNGTC 1 cut(s) 92
Acc16I TGCGCA 1 cut(s) 3204
Acc36I ACCTGC 1 cut(s) 1514
Acc65I GGTACC 1 cut(s) 2760
AccB1I GGYRCC 1 cut(s) 2760
AccI GTMKAC 1 cut(s) 2097
AccII CGCG 9 cut(s) 98, 218, 230, 314, 413, 480, 735, 1590, 3232
AccIII TCCGGA 2 cut(s) 120, 538
AclWI GGATC 7 cut(s) 527, 1612, 2298, 2392, 2660, 2858, 2945
AcoI YGGCCR 2 cut(s) 831, 2101
AcsI RAATTY 6 cut(s) 19, 1060, 1119, 1359, 1772, 3023
AcuI CTGAAG 6 cut(s) 893, 1037, 1208, 1716, 2621, 2814
AcyI GRCGYC 2 cut(s) 236, 2422
AfaI GTAC 5 cut(s) 454, 943, 2353, 2762, 3210
AgeI ACCGGT 1 cut(s) 3308
AjiI CACGTC 1 cut(s) 637
AjnI CCWGG 4 cut(s) 801, 981, 2417, 3259
AjuI GAANNNNNNNTTGG 4 cut(s) 1758, 1790, 2001, 2033
AleI CACNNNNGTG 1 cut(s) 307
Alw21I GWGCWC 3 cut(s) 39, 263, 366
Alw26I GTCTC 6 cut(s) 183, 338, 465, 507, 595, 2767
AlwI GGATC 7 cut(s) 527, 1612, 2298, 2392, 2660, 2858, 2945
AlwNI CAGNNNCTG 2 cut(s) 698, 2867
Ama87I CYCGRG 3 cut(s) 844, 1833, 3004
Aor13HI TCCGGA 2 cut(s) 120, 538
AoxI GGCC 9 cut(s) 105, 457, 661, 831, 1778, 2101, 2976, 3102, 3153
ApoI RAATTY 6 cut(s) 19, 1060, 1119, 1359, 1772, 3023
ArsI GACNNNNNNTTYG 2 cut(s) 2018, 2050
AsiGI ACCGGT 1 cut(s) 3308
Asp700I GAANNNNTTC 2 cut(s) 224, 1515
Asp718I GGTACC 1 cut(s) 2760
AspLEI GCGC 8 cut(s) 314, 388, 482, 735, 851, 1325, 1707, 3205
AsuC2I CCSGG 1 cut(s) 376
AsuNHI GCTAGC 1 cut(s) 325
AvaI CYCGRG 3 cut(s) 844, 1833, 3004
AvaII GGWCC 6 cut(s) 496, 805, 1077, 1228, 2401, 2842
AxyI CCTNAGG 2 cut(s) 461, 2803
BaeGI GKGCMC 1 cut(s) 1150
BaeI ACNNNNGTAYC 2 cut(s) 2335, 2368
BanI GGYRCC 1 cut(s) 2760
BanII GRGCYC 2 cut(s) 39, 366
BarI GAAGNNNNNNTAC 2 cut(s) 1707, 1739
BauI CACGAG 5 cut(s) 206, 468, 893, 1149, 1479
BbsI GAAGAC 8 cut(s) 623, 1025, 1248, 1542, 2501, 2543, 2585, 3296
Bbv12I GWGCWC 3 cut(s) 39, 263, 366
BccI CCATC 2 cut(s) 2483, 2693
BceAI ACGGC 5 cut(s) 411, 638, 648, 805, 2116
BcgI CGANNNNNNTGC 4 cut(s) 1081, 1115, 1941, 1975
BciT130I CCWGG 4 cut(s) 803, 983, 2419, 3261
BciVI GTATCC 1 cut(s) 2806
BclI TGATCA 1 cut(s) 171
BcnI CCSGG 1 cut(s) 376
BcoDI GTCTC 6 cut(s) 183, 338, 465, 507, 595, 2767
BfaI CTAG 4 cut(s) 326, 629, 2946, 3168
BfmI CTRYAG 1 cut(s) 1878
BfoI RGCGCY 2 cut(s) 389, 852
BfuAI ACCTGC 1 cut(s) 1514
BfuI GTATCC 1 cut(s) 2806
BglI GCCNNNNNGGC 2 cut(s) 2167, 3161
Bme1390I CCNGG 5 cut(s) 376, 803, 983, 2419, 3261
Bme18I GGWCC 6 cut(s) 496, 805, 1077, 1228, 2401, 2842
BmeT110I CYCGRG 3 cut(s) 844, 1833, 3004
BmgBI CACGTC 1 cut(s) 637
BmiI GGNNCC 6 cut(s) 751, 807, 1079, 2762, 2789, 2843
BmrFI CCNGG 5 cut(s) 376, 803, 983, 2419, 3261
BmtI GCTAGC 1 cut(s) 329
BpiI GAAGAC 8 cut(s) 623, 1025, 1248, 1542, 2501, 2543, 2585, 3296
BplI GAGNNNNNCTC 4 cut(s) 1481, 1513, 2762, 2794
BpmI CTGGAG 1 cut(s) 2744
BpuEI CTTGAG 3 cut(s) 1483, 1685, 2106
BpuMI CCSGG 1 cut(s) 376
Bsa29I ATCGAT 1 cut(s) 522
BsaHI GRCGYC 2 cut(s) 236, 2422
BsaI GGTCTC 1 cut(s) 2767
BsaWI WCCGGW 5 cut(s) 120, 255, 304, 538, 3308
BsaXI ACNNNNNCTCC 6 cut(s) 299, 329, 1565, 1595, 3053, 3083
Bse118I RCCGGY 4 cut(s) 107, 1780, 1918, 3308
Bse1I ACTGG 4 cut(s) 1258, 1434, 2292, 3165
Bse21I CCTNAGG 2 cut(s) 461, 2803
Bse3DI GCAATG 1 cut(s) 320
BseAI TCCGGA 2 cut(s) 120, 538
BseBI CCWGG 4 cut(s) 803, 983, 2419, 3261
BseCI ATCGAT 1 cut(s) 522
BseGI GGATG 8 cut(s) 547, 969, 1102, 1598, 2114, 2575, 2617, 2819
BseMI GCAATG 1 cut(s) 320
BseMII CTCAG 7 cut(s) 452, 622, 946, 1947, 2332, 2404, 2817
BseNI ACTGG 4 cut(s) 1258, 1434, 2292, 3165
BseRI GAGGAG 6 cut(s) 266, 1573, 1649, 1877, 2236, 2770
BseSI GKGCMC 1 cut(s) 1150
BseYI CCCAGC 1 cut(s) 1341
BsgI GTGCAG 3 cut(s) 374, 3170, 3305
Bsh1236I CGCG 9 cut(s) 98, 218, 230, 314, 413, 480, 735, 1590, 3232
BshFI GGCC 9 cut(s) 107, 459, 663, 833, 1780, 2103, 2978, 3104, 3155
BshNI GGYRCC 1 cut(s) 2760
BshTI ACCGGT 1 cut(s) 3308
BshVI ATCGAT 1 cut(s) 522
BsiHKAI GWGCWC 3 cut(s) 39, 263, 366
BsiHKCI CYCGRG 3 cut(s) 844, 1833, 3004
BsiWI CGTACG 1 cut(s) 452
BslFI GGGAC 4 cut(s) 784, 791, 2351, 2696
BsmAI GTCTC 6 cut(s) 183, 338, 465, 507, 595, 2767
BsmBI CGTCTC 1 cut(s) 465
BsmFI GGGAC 4 cut(s) 784, 791, 2351, 2696
BsmI GAATGC 2 cut(s) 2444, 2833
BsnI GGCC 9 cut(s) 107, 459, 663, 833, 1780, 2103, 2978, 3104, 3155
Bso31I GGTCTC 1 cut(s) 2767
BsoBI CYCGRG 3 cut(s) 844, 1833, 3004
Bsp1286I GDGCHC 4 cut(s) 39, 263, 366, 1150
Bsp13I TCCGGA 2 cut(s) 120, 538
Bsp19I CCATGG 2 cut(s) 1905, 2875
BspANI GGCC 9 cut(s) 107, 459, 663, 833, 1780, 2103, 2978, 3104, 3155
BspCNI CTCAG 7 cut(s) 453, 621, 945, 1948, 2331, 2403, 2816
BspDI ATCGAT 1 cut(s) 522
BspEI TCCGGA 2 cut(s) 120, 538
BspFNI CGCG 9 cut(s) 98, 218, 230, 314, 413, 480, 735, 1590, 3232
BspHI TCATGA 1 cut(s) 2512
BspLI GGNNCC 6 cut(s) 751, 807, 1079, 2762, 2789, 2843
BspMAI CTGCAG 1 cut(s) 1882
BspMI ACCTGC 1 cut(s) 1514
BspOI GCTAGC 1 cut(s) 329
BspPI GGATC 7 cut(s) 527, 1612, 2298, 2392, 2660, 2858, 2945
BspQI GCTCTTC 2 cut(s) 920, 1074
BspT107I GGYRCC 1 cut(s) 2760
BspTNI GGTCTC 1 cut(s) 2767
BsrDI GCAATG 1 cut(s) 320
BsrFI RCCGGY 4 cut(s) 107, 1780, 1918, 3308
BsrI ACTGG 4 cut(s) 1258, 1434, 2292, 3165
BssAI RCCGGY 4 cut(s) 107, 1780, 1918, 3308
BssNI GRCGYC 2 cut(s) 236, 2422
BssSI CACGAG 5 cut(s) 206, 468, 893, 1149, 1479
BssT1I CCWWGG 4 cut(s) 102, 1905, 2145, 2875
Bst2BI CACGAG 5 cut(s) 206, 468, 893, 1149, 1479
Bst2UI CCWGG 4 cut(s) 803, 983, 2419, 3261
Bst4CI ACNGT 7 cut(s) 141, 265, 598, 694, 1089, 1290, 2227
Bst6I CTCTTC 2 cut(s) 920, 1074
BstACI GRCGYC 2 cut(s) 236, 2422
BstAPI GCANNNNNTGC 2 cut(s) 488, 1408
BstC8I GCNNGC 9 cut(s) 109, 327, 489, 1912, 1916, 2159, 2533, 3079, 3153
BstDSI CCRYGG 5 cut(s) 852, 1905, 2223, 2875, 3230
BstF5I GGATG 8 cut(s) 547, 969, 1102, 1598, 2114, 2575, 2617, 2819
BstFNI CGCG 9 cut(s) 98, 218, 230, 314, 413, 480, 735, 1590, 3232
BstH2I RGCGCY 2 cut(s) 389, 852
BstHHI GCGC 8 cut(s) 314, 388, 482, 735, 851, 1325, 1707, 3205
BstMAI GTCTC 6 cut(s) 183, 338, 465, 507, 595, 2767
BstNI CCWGG 4 cut(s) 803, 983, 2419, 3261
BstNSI RCATGY 1 cut(s) 2366
BstSCI CCNGG 5 cut(s) 374, 801, 981, 2417, 3259
BstSFI CTRYAG 1 cut(s) 1878
BstSLI GKGCMC 1 cut(s) 1150
BstUI CGCG 9 cut(s) 98, 218, 230, 314, 413, 480, 735, 1590, 3232
BstV2I GAAGAC 8 cut(s) 623, 1025, 1248, 1542, 2501, 2543, 2585, 3296
BstX2I RGATCY 3 cut(s) 1604, 2384, 2863
BstYI RGATCY 3 cut(s) 1604, 2384, 2863
Bsu15I ATCGAT 1 cut(s) 522
Bsu36I CCTNAGG 2 cut(s) 461, 2803
BsuI GTATCC 1 cut(s) 2806
BsuRI GGCC 9 cut(s) 107, 459, 663, 833, 1780, 2103, 2978, 3104, 3155
BsuTUI ATCGAT 1 cut(s) 522
BtgI CCRYGG 5 cut(s) 852, 1905, 2223, 2875, 3230
BtgZI GCGATG 1 cut(s) 750
BtrI CACGTC 1 cut(s) 637
BtsCI GGATG 8 cut(s) 547, 969, 1102, 1598, 2114, 2575, 2617, 2819
BtsI GCAGTG 1 cut(s) 943
BtsIMutI CAGTG 8 cut(s) 616, 943, 1286, 1441, 1545, 1740, 2326, 3299
BveI ACCTGC 1 cut(s) 1514
Cac8I GCNNGC 9 cut(s) 109, 327, 489, 1912, 1916, 2159, 2533, 3079, 3153
CaiI CAGNNNCTG 2 cut(s) 698, 2867
CciI TCATGA 1 cut(s) 2512
CfoI GCGC 8 cut(s) 314, 388, 482, 735, 851, 1325, 1707, 3205
Cfr10I RCCGGY 4 cut(s) 107, 1780, 1918, 3308
Cfr42I CCGCGG 1 cut(s) 3233
ClaI ATCGAT 1 cut(s) 522
CseI GACGC 5 cut(s) 87, 225, 303, 486, 2411
CsiI ACCWGGT 1 cut(s) 3259
Csp6I GTAC 5 cut(s) 453, 942, 2352, 2761, 3209
CspAI ACCGGT 1 cut(s) 3308
CspCI CAANNNNNGTGG 2 cut(s) 2417, 2452
CviQI GTAC 5 cut(s) 453, 942, 2352, 2761, 3209
DraI TTTAAA 1 cut(s) 1858
DrdI GACNNNNNNGTC 1 cut(s) 92
DseDI GACNNNNNNGTC 1 cut(s) 92
EaeI YGGCCR 2 cut(s) 831, 2101
Eam1104I CTCTTC 2 cut(s) 920, 1074
EarI CTCTTC 2 cut(s) 920, 1074
Ecl136II GAGCTC 2 cut(s) 37, 364
Eco130I CCWWGG 4 cut(s) 102, 1905, 2145, 2875
Eco24I GRGCYC 2 cut(s) 39, 366
Eco31I GGTCTC 1 cut(s) 2767
Eco47I GGWCC 6 cut(s) 496, 805, 1077, 1228, 2401, 2842
Eco53kI GAGCTC 2 cut(s) 37, 364
Eco57I CTGAAG 6 cut(s) 893, 1037, 1208, 1716, 2621, 2814
Eco81I CCTNAGG 2 cut(s) 461, 2803
Eco88I CYCGRG 3 cut(s) 844, 1833, 3004
EcoICRI GAGCTC 2 cut(s) 37, 364
EcoO109I RGGNCCY 3 cut(s) 1228, 2842, 2977
EcoRII CCWGG 4 cut(s) 801, 981, 2417, 3259
EcoT14I CCWWGG 4 cut(s) 102, 1905, 2145, 2875
EcoT22I ATGCAT 1 cut(s) 2446
EcoT38I GRGCYC 2 cut(s) 39, 366
ErhI CCWWGG 4 cut(s) 102, 1905, 2145, 2875
Esp3I CGTCTC 1 cut(s) 465
FalI AAGNNNNNCTT 2 cut(s) 1694, 1726
FaqI GGGAC 4 cut(s) 784, 791, 2351, 2696
FauNDI CATATG 1 cut(s) 643
FbaI TGATCA 1 cut(s) 171
FblI GTMKAC 1 cut(s) 2097
FokI GGATG 8 cut(s) 554, 956, 1089, 1605, 2101, 2582, 2624, 2806
FriOI GRGCYC 2 cut(s) 39, 366
FspBI CTAG 4 cut(s) 326, 629, 2946, 3168
FspI TGCGCA 1 cut(s) 3204
GlaI GCGC 8 cut(s) 313, 387, 481, 734, 850, 1324, 1706, 3204
GsaI CCCAGC 1 cut(s) 1345
GsuI CTGGAG 1 cut(s) 2744
HaeII RGCGCY 2 cut(s) 389, 852
HaeIII GGCC 9 cut(s) 107, 459, 663, 833, 1780, 2103, 2978, 3104, 3155
HgaI GACGC 5 cut(s) 87, 225, 303, 486, 2411
HhaI GCGC 8 cut(s) 314, 388, 482, 735, 851, 1325, 1707, 3205
Hin1I GRCGYC 2 cut(s) 236, 2422
Hin6I GCGC 8 cut(s) 312, 386, 480, 733, 849, 1323, 1705, 3203
HinP1I GCGC 8 cut(s) 312, 386, 480, 733, 849, 1323, 1705, 3203
Hpy166II GTNNAC 5 cut(s) 712, 1478, 1738, 2098, 3211
Hpy8I GTNNAC 5 cut(s) 712, 1478, 1738, 2098, 3211
Hpy99I CGWCG 7 cut(s) 34, 89, 98, 116, 319, 371, 866
HpyCH4III ACNGT 7 cut(s) 141, 265, 598, 694, 1089, 1290, 2227
HpyCH4IV ACGT 3 cut(s) 93, 636, 2209
HpySE526I ACGT 3 cut(s) 93, 636, 2209
Hsp92I GRCGYC 2 cut(s) 236, 2422
HspAI GCGC 8 cut(s) 312, 386, 480, 733, 849, 1323, 1705, 3203
Kpn2I TCCGGA 2 cut(s) 120, 538
KpnI GGTACC 1 cut(s) 2764
KroI GCCGGC 1 cut(s) 107
KroNI GCCGGC 1 cut(s) 109
Ksp22I TGATCA 1 cut(s) 171
KspI CCGCGG 1 cut(s) 3233
LguI GCTCTTC 2 cut(s) 920, 1074
MabI ACCWGGT 1 cut(s) 3259
MaeI CTAG 4 cut(s) 326, 629, 2946, 3168
MaeII ACGT 3 cut(s) 93, 636, 2209
MfeI CAATTG 1 cut(s) 2002
MflI RGATCY 3 cut(s) 1604, 2384, 2863
MhlI GDGCHC 4 cut(s) 39, 263, 366, 1150
MlyI GAGTC 7 cut(s) 205, 293, 529, 1491, 1574, 1733, 1967
MmeI TCCRAC 9 cut(s) 319, 705, 1218, 2061, 2209, 2367, 2599, 2672, 2714
Mph1103I ATGCAT 1 cut(s) 2446
MroI TCCGGA 2 cut(s) 120, 538
MroNI GCCGGC 1 cut(s) 107
MroXI GAANNNNTTC 2 cut(s) 224, 1515
MseI TTAA 5 cut(s) 1137, 1178, 1275, 1857, 2358
MslI CAYNNNNRTG 1 cut(s) 307
MspA1I CMGCKG 5 cut(s) 352, 1883, 1955, 2959, 3232
MspR9I CCNGG 5 cut(s) 376, 803, 983, 2419, 3261
MunI CAATTG 1 cut(s) 2002
Mva1269I GAATGC 2 cut(s) 2444, 2833
MvaI CCWGG 4 cut(s) 803, 983, 2419, 3261
MvnI CGCG 9 cut(s) 98, 218, 230, 314, 413, 480, 735, 1590, 3232
NaeI GCCGGC 1 cut(s) 109
NciI CCSGG 1 cut(s) 376
NcoI CCATGG 2 cut(s) 1905, 2875
NdeI CATATG 1 cut(s) 643
NgoMIV GCCGGC 1 cut(s) 107
NheI GCTAGC 1 cut(s) 325
NlaIV GGNNCC 6 cut(s) 751, 807, 1079, 2762, 2789, 2843
NmeAIII GCCGAG 2 cut(s) 895, 1585
NmuCI GTSAC 8 cut(s) 208, 632, 763, 960, 1849, 2321, 2393, 2423
NsbI TGCGCA 1 cut(s) 3204
NsiI ATGCAT 1 cut(s) 2446
NspI RCATGY 1 cut(s) 2366
OliI CACNNNNGTG 1 cut(s) 307
PaeR7I CTCGAG 2 cut(s) 844, 1833
PagI TCATGA 1 cut(s) 2512
PaqCI CACCTGC 1 cut(s) 1514
PciSI GCTCTTC 2 cut(s) 920, 1074
PcsI WCGNNNNNNNCGW 3 cut(s) 90, 156, 366
PctI GAATGC 2 cut(s) 2444, 2833
PdiI GCCGGC 1 cut(s) 109
PdmI GAANNNNTTC 2 cut(s) 224, 1515
Pfl23II CGTACG 1 cut(s) 452
PfoI TCCNGGA 1 cut(s) 374
PinAI ACCGGT 1 cut(s) 3308
PleI GAGTC 7 cut(s) 205, 293, 529, 1490, 1574, 1733, 1966
PpsI GAGTC 7 cut(s) 205, 293, 529, 1490, 1574, 1733, 1966
PpuMI RGGWCCY 2 cut(s) 1228, 2842
Psp124BI GAGCTC 2 cut(s) 39, 366
Psp5II RGGWCCY 2 cut(s) 1228, 2842
Psp6I CCWGG 4 cut(s) 801, 981, 2417, 3259
PspFI CCCAGC 1 cut(s) 1341
PspGI CCWGG 4 cut(s) 801, 981, 2417, 3259
PspLI CGTACG 1 cut(s) 452
PspN4I GGNNCC 6 cut(s) 751, 807, 1079, 2762, 2789, 2843
PspPPI RGGWCCY 2 cut(s) 1228, 2842
PspXI VCTCGAGB 1 cut(s) 844
PstI CTGCAG 1 cut(s) 1882
PstNI CAGNNNCTG 2 cut(s) 698, 2867
PsuI RGATCY 3 cut(s) 1604, 2384, 2863
PvuII CAGCTG 2 cut(s) 1883, 1955
RsaI GTAC 5 cut(s) 454, 943, 2353, 2762, 3210
RsaNI GTAC 5 cut(s) 453, 942, 2352, 2761, 3209
RseI CAYNNNNRTG 1 cut(s) 307
SacI GAGCTC 2 cut(s) 39, 366
SacII CCGCGG 1 cut(s) 3233
SapI GCTCTTC 2 cut(s) 920, 1074
SaqAI TTAA 5 cut(s) 1137, 1178, 1275, 1857, 2358
SchI GAGTC 7 cut(s) 205, 293, 529, 1491, 1574, 1733, 1967
ScrFI CCNGG 5 cut(s) 376, 803, 983, 2419, 3261
SduI GDGCHC 4 cut(s) 39, 263, 366, 1150
SexAI ACCWGGT 1 cut(s) 3259
SfcI CTRYAG 1 cut(s) 1878
Sfr274I CTCGAG 2 cut(s) 844, 1833
Sfr303I CCGCGG 1 cut(s) 3233
SgrBI CCGCGG 1 cut(s) 3233
SinI GGWCC 6 cut(s) 496, 805, 1077, 1228, 2401, 2842
SlaI CTCGAG 2 cut(s) 844, 1833
SmiMI CAYNNNNRTG 1 cut(s) 307
SmlI CTYRAG 5 cut(s) 844, 1498, 1700, 1833, 2121
SmoI CTYRAG 5 cut(s) 844, 1498, 1700, 1833, 2121
SspMI CTAG 4 cut(s) 326, 629, 2946, 3168
SstI GAGCTC 2 cut(s) 39, 366
StyD4I CCNGG 5 cut(s) 374, 801, 981, 2417, 3259
StyI CCWWGG 4 cut(s) 102, 1905, 2145, 2875
TaaI ACNGT 7 cut(s) 141, 265, 598, 694, 1089, 1290, 2227
TaiI ACGT 3 cut(s) 96, 639, 2212
TatI WGTACW 1 cut(s) 3208
TauI GCSGC 6 cut(s) 401, 416, 683, 833, 2962, 3138
Tru1I TTAA 5 cut(s) 1137, 1178, 1275, 1857, 2358
Tru9I TTAA 5 cut(s) 1137, 1178, 1275, 1857, 2358
TscAI CASTG 8 cut(s) 616, 943, 1293, 1441, 1552, 1740, 2326, 3306
TseFI GTSAC 8 cut(s) 208, 632, 763, 960, 1849, 2321, 2393, 2423
Tsp45I GTSAC 8 cut(s) 208, 632, 763, 960, 1849, 2321, 2393, 2423
TspGWI ACGGA 5 cut(s) 47, 489, 869, 959, 2624
TspRI CASTG 8 cut(s) 616, 943, 1293, 1441, 1552, 1740, 2326, 3306
VpaK11BI GGWCC 6 cut(s) 496, 805, 1077, 1228, 2401, 2842
XapI RAATTY 6 cut(s) 19, 1060, 1119, 1359, 1772, 3023
XceI RCATGY 1 cut(s) 2366
XcmI CCANNNNNNNNNTGG 1 cut(s) 559
XhoI CTCGAG 2 cut(s) 844, 1833
XmiI GTMKAC 1 cut(s) 2097
XmnI GAANNNNTTC 2 cut(s) 224, 1515
XspI CTAG 4 cut(s) 326, 629, 2946, 3168
Zsp2I ATGCAT 1 cut(s) 2446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.