Rh2BG618300

OST-HTH/LOTUS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000679
Physical Location & Seq
Reverse (-)
84117505 .. 84122407
4903 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG618300.1

Sequence Viewer

Length: 2715 bp
ATGAATGATCAATCTCAGGATTTGAGCACGGTTAAACTTCTGAGTGAAAAAACCAAGGAGATTGCTGACGTGGCGTATGGTTACGCCGTTAAAGGCCGTGTAGCTTCTCTGACGGCGCTGCTCATCGTCGCCGCCGAGAAGGTTAACGATTCCGTCTTGGAGTTGCGTGATTCCGAATCGGGGACCAAGGAGAAGTTGACTATCTACGAATGGCTTATAAGGGAGGCTTTGTCCATGGGCGGCCGTACAGCGTTGCCGTTGAGGGCGGCCAAACGCGCCTCCACGGCTACGGAGGGGGAAAACTCGGAGAGGAGGAAGCTCGTGCTTCGAGAGATAGAACTGCTTCAGCTCTTCGGAGCTGTAGCTCAGAGCAGCGGTGGTGGGGACAAGAAGGTGACTTCGCCACTCATCCGTGCAGCTCAGGGAGGAGACGAAGCAGTTATTCAGCTGCTTTTGAAGACAAATATAGATGTCAATGATGCTGATGCAGATGGAAATTCTGCTCTTCATTGGATCCTTAAATTGTCCAGATTGTTGTGTCCCCAACAGATCAAAATTGTATTGCTTCTTATTGATCATGGTGCCCGAGTAAGCCAAAGGAATAGATTGGGGTTAACAGCATTTCATATTGCTGCCGGTAATGGTAATTCAGAGGCACTTGAGGTCCTTCTGTTGGAAGACCCAGTTGGCGTCCAATATAGGACGGAAATGAAAGAAACGCCCCTGTTTTTCGCTGTGAAGAATGAGAGTAAGGAGTGTACTGAGCTTCTTTTAACCTGGGGAGCAAATAGTGAAGTCCTTAATCTGCGTAGGCAAAGAGCTATAGACCTGGCAACCTCTCAAGATATGCGTTACATATTGCTGAACCCAACCACTGTTAGCCTCATTAATCACGCTTTCCCCAATCAACATAAATGTACGGCTTTAGAGTTGACAGATGAAGACACCGCTAATGAAAGAAATGTTAACTCAAGCACCAAGGCAGAGGTTTGTAAGTACTTTGACTCGCATCGAGGATGTGTCAGAGGAGCTAAGTGCTTTTATGCACATTGTGAGGAGGAGTCTCGGAAAGTGAAGGAGGGAGCAGACCGGAGTCATTTTTCTGCTGCTAAACTACTCAAACACAAAATCTTTGTTGGTGGACTTCCTCCATCAGTGGACTCTGATGATTTGGGAAAGTTCTGTGAAGAGGAGTTTGGATCAGTGGACGATGCTATAGTCATTGTAACTCAAACAGAAAACAAAATACAATCTCGAGGCTTTGGTTTTGTCACATTCAGAGAGGAGAAATCTGCTTCAGAAGCTATGCAAGCGCACTACATCACCATGAGAGGCAAGAAAGTTGAAATAAAAAGTTTAATTCCAAAATTGGTTTTGGGAGCTGAGTTTGAGAAGATGTCACCTCAACAACGACAACAAGATAAGAATATTCAAAATCAGCTTCCACAGCAGAAGTCGAACGAGAATATTATGGAAGGTGATAACCCTGAACAAGGTTCTTGGGCTGATAGATTAGTCCTTGGACAACCAGTGGCGTGTTCCACTGAACCTCAAGTTACCAAGAGCTCTGAGGATCCAAGCACTCCTACATGGCTAAAGGTTTTCAAGAAGTGGTTTCCTGGGTTCTTACAAGGTCTGTCAAAGCATTCAAATTATGCTCTGTCTTCTCTAAAAGCTGACTTCAGGGCTAAATTTGGGTTAGAACTGGATCACTCATCTGTGGGCTACTCTAAGCTCAGTGACTTTGTGAAATGTTTCTCTGATTTATGTACTGTTGAGATTCTCCCCATCTGCAAACGTGGAACTCCAACTCACGTGATCCTCCGGCCAAAATTTTCTAGGCCTCAACGCCGACTCCTTCCTACACTGAGAACACCTCACACTCTGTCTCTGTCTGCACTGCTTGTCAAAGGCGGTGATACAAAGTGTCTTTTGGACCTCTCAAGTGGCGATTGTGGTGATTCAAAGTGTCTTCAGGACCATTCGAAGTGTTTTCAGGACCTTTCAGTTAACAATGGCAGTGACTCAAAGTGTTTTCAGAACCTTACAATTGAAAGCATTAGTGATACAAAGTGTCTTCAGGACATTTCAGCTGACAATGCTGCTGATTCAAAGTGCCTTCAGGACCTTTCACTTGACTTTAGTGGTGATTCAAACTGTATTGAGGACCTTTCCATTTGCAATGCTGGTGAGAAGTTTGGCTCAAGTGTCACTAGCCATGGGCAAGAGAAACCCTCCTCTGGGTATCCTGAAATGAACTCTGCAGAGGATAAAGGACAATGTGTGCACCTGAGGTTCCTTCAATTCTTAAAACCAGATCCTCTATTCTATGGTCAGAAAGAAACTGACGTGCTTGCCGGAGAGACTGACAATGAAAAGGGACAGTGCAGTGCGGGGGATAAAGGAAGTATAAATATTAGGGATCCAAGACGGCATCTCGTTTTGGAGTCCCTGTCCAAGAAATGGAGCAAATCCTACTTCCTTCGTGAATTTGATTTCTACAAGATTTACAAGGAAAGATTATCGGTAAGAAGGTGTTTTGCATGCAACCAGCAGAAGATGCTATGGGCCAATTTCCCTTGTAAACACTTGCTGTGGTGCACTGACTGTAAGTTGCGGGCAATAGAGGCTTCTGGTCTTTTCCCACACAAATGCGTGCTGTGTGACACAGAAGTACAGAAAATGGATTTAGTACTTCCATTTACTAGTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 OST-HTH RRM_1

Protein Analysis

904

Amino Acids

100.55

Weight (kDa)

6.72

Isoelectric Point (pI)

45.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 131 - 200 7.1e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 184 - 263 1.2e-10 Ankyrin repeats (3 copies)
Ank_4 PF13637 184 - 224 3.1e-06 Ankyrin repeats (many copies)
Ank_5 PF13857 190 - 242 1e-05 Ankyrin repeats (many copies)
RRM_1 PF00076 377 - 448 7.8e-12 RNA recognition motif
OST-HTH PF12872 531 - 593 6.1e-08 OST-HTH/LOTUS domain
zf-C3HC4_3 PF13920 844 - 895 7.5e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 218
AccB1I GGYRCC 1 cut(s) 581
AccB7I CCANNNNNTGG 1 cut(s) 2463
AccII CGCG 1 cut(s) 276
AciI CCGC 8 cut(s) 132, 240, 266, 375, 948, 1914, 2393, 2617
AcoI YGGCCR 3 cut(s) 241, 267, 1826
AcsI RAATTY 4 cut(s) 496, 1691, 1832, 2489
AcuI CTGAAG 6 cut(s) 329, 1281, 1666, 1958, 2063, 2105
AcvI CACGTG 1 cut(s) 1816
AcyI GRCGYC 1 cut(s) 690
AdeI CACNNNGTG 1 cut(s) 1052
AfaI GTAC 7 cut(s) 247, 760, 919, 998, 1771, 2676, 2694
AfiI CCNNNNNNNGG 6 cut(s) 180, 673, 1493, 2240, 2241, 2463
AhlI ACTAGT 1 cut(s) 2705
AjiI CACGTC 2 cut(s) 70, 2350
AjnI CCWGG 3 cut(s) 776, 828, 1618
AjuI GAANNNNNNNTTGG 4 cut(s) 669, 701, 1179, 1211
Alw21I GWGCWC 4 cut(s) 29, 1568, 2289, 2603
Alw26I GTCTC 4 cut(s) 423, 1068, 1893, 2357
Alw44I GTGCAC 2 cut(s) 2285, 2599
Ama87I CYCGRG 2 cut(s) 585, 1254
AoxI GGCC 6 cut(s) 94, 241, 267, 1826, 1841, 2568
ApaLI GTGCAC 2 cut(s) 2285, 2599
ApeKI GCWGC 7 cut(s) 118, 372, 416, 448, 632, 1106, 2102
ApoI RAATTY 4 cut(s) 496, 1691, 1832, 2489
AseI ATTAAT 1 cut(s) 888
Asp700I GAANNNNTTC 2 cut(s) 342, 1754
AspLEI GCGC 3 cut(s) 118, 278, 1315
AspS9I GGNCC 8 cut(s) 183, 664, 1936, 1978, 1999, 2125, 2167, 2568
AsuHPI GGTGA 8 cut(s) 406, 1315, 1392, 1490, 1928, 1970, 2159, 2201
AsuII TTCGAA 1 cut(s) 1985
AvaI CYCGRG 2 cut(s) 585, 1254
AvaII GGWCC 7 cut(s) 183, 664, 1936, 1978, 1999, 2125, 2167
AxyI CCTNAGG 1 cut(s) 2291
BaeGI GKGCMC 3 cut(s) 586, 2289, 2603
BamHI GGATCC 3 cut(s) 513, 1573, 2422
BanI GGYRCC 1 cut(s) 581
BanII GRGCYC 1 cut(s) 1568
BauI CACGAG 1 cut(s) 320
BbrPI CACGTG 1 cut(s) 1816
BbsI GAAGAC 6 cut(s) 464, 684, 948, 1656, 1964, 2069
Bbv12I GWGCWC 4 cut(s) 29, 1568, 2289, 2603
BbvI GCAGC 7 cut(s) 105, 384, 428, 435, 619, 1093, 2089
BccI CCATC 3 cut(s) 485, 1159, 1796
BceAI ACGGC 8 cut(s) 71, 81, 129, 228, 241, 300, 936, 2447
BciT130I CCWGG 3 cut(s) 778, 830, 1620
BciVI GTATCC 1 cut(s) 2256
BclI TGATCA 2 cut(s) 7, 574
BcoDI GTCTC 4 cut(s) 423, 1068, 1893, 2357
BcuI ACTAGT 1 cut(s) 2705
BfaI CTAG 3 cut(s) 1839, 2214, 2706
BfmI CTRYAG 4 cut(s) 360, 822, 1215, 2262
BfoI RGCGCY 1 cut(s) 119
BfuI GTATCC 1 cut(s) 2256
BglI GCCNNNNNGGC 1 cut(s) 284
BmcAI AGTACT 2 cut(s) 998, 2694
Bme1390I CCNGG 3 cut(s) 778, 830, 1620
Bme18I GGWCC 7 cut(s) 183, 664, 1936, 1978, 1999, 2125, 2167
BmeT110I CYCGRG 2 cut(s) 585, 1254
BmgBI CACGTC 2 cut(s) 70, 2350
BmgT120I GGNCC 8 cut(s) 183, 664, 1936, 1978, 1999, 2125, 2167, 2568
BmiI GGNNCC 6 cut(s) 184, 515, 583, 1575, 2297, 2424
BmrFI CCNGG 3 cut(s) 778, 830, 1620
BmrI ACTGGG 1 cut(s) 677
BmsI GCATC 6 cut(s) 469, 475, 1018, 1201, 2443, 2550
BmuI ACTGGG 1 cut(s) 677
BoxI GACNNNNGTC 1 cut(s) 1092
BpiI GAAGAC 6 cut(s) 464, 684, 948, 1656, 1964, 2069
BplI GAGNNNNNCTC 4 cut(s) 1861, 1893, 2219, 2251
Bpu10I CCTNAGC 1 cut(s) 420
Bpu14I TTCGAA 1 cut(s) 1985
BpuEI CTTGAG 6 cut(s) 680, 825, 955, 1536, 1927, 2188
BsaAI YACGTR 1 cut(s) 1816
BsaHI GRCGYC 1 cut(s) 690
BsaJI CCNNGG 9 cut(s) 54, 186, 234, 282, 777, 978, 1519, 1619, 2218
BsaWI WCCGGW 1 cut(s) 1089
BsaXI ACNNNNNCTCC 6 cut(s) 215, 245, 1839, 1869, 2438, 2468
Bsc4I CCNNNNNNNGG 6 cut(s) 180, 673, 1493, 2240, 2241, 2463
Bse118I RCCGGY 1 cut(s) 635
Bse1I ACTGG 3 cut(s) 683, 1529, 1710
Bse21I CCTNAGG 1 cut(s) 2291
Bse3DI GCAATG 1 cut(s) 2188
BseBI CCWGG 3 cut(s) 778, 830, 1620
BseDI CCNNGG 9 cut(s) 54, 186, 234, 282, 777, 978, 1519, 1619, 2218
BseGI GGATG 2 cut(s) 408, 1022
BseLI CCNNNNNNNGG 6 cut(s) 180, 673, 1493, 2240, 2241, 2463
BseMI GCAATG 1 cut(s) 2188
BseNI ACTGG 3 cut(s) 683, 1529, 1710
BseRI GAGGAG 8 cut(s) 325, 441, 1041, 1070, 1073, 1205, 1298, 2227
BseSI GKGCMC 3 cut(s) 586, 2289, 2603
BseX3I CGGCCG 1 cut(s) 241
BseXI GCAGC 7 cut(s) 105, 384, 428, 435, 619, 1093, 2089
BsgI GTGCAG 3 cut(s) 435, 1881, 2407
Bsh1236I CGCG 1 cut(s) 276
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 6 cut(s) 96, 243, 269, 1828, 1843, 2570
BshNI GGYRCC 1 cut(s) 581
BsiEI CGRYCG 1 cut(s) 244
BsiHKAI GWGCWC 4 cut(s) 29, 1568, 2289, 2603
BsiHKCI CYCGRG 2 cut(s) 585, 1254
BsiSI CCGG 4 cut(s) 636, 1090, 1825, 2358
BslFI GGGAC 5 cut(s) 196, 398, 525, 2394, 2434
BslI CCNNNNNNNGG 6 cut(s) 180, 673, 1493, 2240, 2241, 2463
BsmAI GTCTC 4 cut(s) 423, 1068, 1893, 2357
BsmBI CGTCTC 1 cut(s) 423
BsmFI GGGAC 5 cut(s) 196, 398, 525, 2394, 2434
BsmI GAATGC 1 cut(s) 1645
BsnI GGCC 6 cut(s) 96, 243, 269, 1828, 1843, 2570
BsoBI CYCGRG 2 cut(s) 585, 1254
Bsp119I TTCGAA 1 cut(s) 1985
Bsp1286I GDGCHC 5 cut(s) 29, 586, 1568, 2289, 2603
Bsp19I CCATGG 2 cut(s) 234, 2218
BspACI CCGC 8 cut(s) 132, 240, 266, 375, 948, 1914, 2393, 2617
BspANI GGCC 6 cut(s) 96, 243, 269, 1828, 1843, 2570
BspFNI CGCG 1 cut(s) 276
BspLI GGNNCC 6 cut(s) 184, 515, 583, 1575, 2297, 2424
BspMAI CTGCAG 1 cut(s) 2266
BspQI GCTCTTC 2 cut(s) 356, 510
BspT104I TTCGAA 1 cut(s) 1985
BspT107I GGYRCC 1 cut(s) 581
BsrDI GCAATG 1 cut(s) 2188
BsrFI RCCGGY 1 cut(s) 635
BsrI ACTGG 3 cut(s) 683, 1529, 1710
BssAI RCCGGY 1 cut(s) 635
BssECI CCNNGG 9 cut(s) 54, 186, 234, 282, 777, 978, 1519, 1619, 2218
BssNI GRCGYC 1 cut(s) 690
BssSI CACGAG 1 cut(s) 320
BssT1I CCWWGG 6 cut(s) 54, 186, 234, 978, 1519, 2218
Bst2BI CACGAG 1 cut(s) 320
Bst2UI CCWGG 3 cut(s) 778, 830, 1620
Bst4CI ACNGT 6 cut(s) 31, 877, 1774, 2159, 2385, 2609
Bst6I CTCTTC 3 cut(s) 356, 510, 1182
BstACI GRCGYC 1 cut(s) 690
BstAPI GCANNNNNTGC 1 cut(s) 2560
BstBAI YACGTR 1 cut(s) 1816
BstBI TTCGAA 1 cut(s) 1985
BstC8I GCNNGC 5 cut(s) 1311, 2355, 2545, 2619, 2657
BstDSI CCRYGG 3 cut(s) 234, 282, 2218
BstENI CCTNNNNNAGG 1 cut(s) 1491
BstF5I GGATG 2 cut(s) 408, 1022
BstFNI CGCG 1 cut(s) 276
BstH2I RGCGCY 1 cut(s) 119
BstHHI GCGC 3 cut(s) 118, 278, 1315
BstMAI GTCTC 4 cut(s) 423, 1068, 1893, 2357
BstMCI CGRYCG 1 cut(s) 244
BstMWI GCNNNNNNNGC 9 cut(s) 71, 275, 284, 1301, 1310, 1447, 2099, 2560, 2627
BstNI CCWGG 3 cut(s) 778, 830, 1620
BstNSI RCATGY 1 cut(s) 2547
BstPAI GACNNNNGTC 1 cut(s) 1092
BstSCI CCNGG 3 cut(s) 776, 828, 1618
BstSFI CTRYAG 4 cut(s) 360, 822, 1215, 2262
BstSLI GKGCMC 3 cut(s) 586, 2289, 2603
BstUI CGCG 1 cut(s) 276
BstV1I GCAGC 7 cut(s) 105, 384, 428, 435, 619, 1093, 2089
BstV2I GAAGAC 6 cut(s) 464, 684, 948, 1656, 1964, 2069
BstX2I RGATCY 4 cut(s) 513, 1573, 2317, 2422
BstYI RGATCY 4 cut(s) 513, 1573, 2317, 2422
BstZI CGGCCG 1 cut(s) 241
Bsu36I CCTNAGG 1 cut(s) 2291
BsuI GTATCC 1 cut(s) 2256
BsuRI GGCC 6 cut(s) 96, 243, 269, 1828, 1843, 2570
BtgI CCRYGG 3 cut(s) 234, 282, 2218
BtrI CACGTC 2 cut(s) 70, 2350
BtsCI GGATG 2 cut(s) 408, 1022
BtsI GCAGTG 3 cut(s) 1898, 2026, 2395
Cac8I GCNNGC 5 cut(s) 1311, 2355, 2545, 2619, 2657
CfoI GCGC 3 cut(s) 118, 278, 1315
Cfr10I RCCGGY 1 cut(s) 635
Cfr13I GGNCC 8 cut(s) 183, 664, 1936, 1978, 1999, 2125, 2167, 2568
CseI GACGC 1 cut(s) 679
Csp6I GTAC 7 cut(s) 246, 759, 918, 997, 1770, 2675, 2693
CviAII CATG 6 cut(s) 235, 578, 1327, 1590, 2219, 2544
CviQI GTAC 7 cut(s) 246, 759, 918, 997, 1770, 2675, 2693
DraIII CACNNNGTG 1 cut(s) 1052
EaeI YGGCCR 3 cut(s) 241, 267, 1826
EagI CGGCCG 1 cut(s) 241
Eam1104I CTCTTC 3 cut(s) 356, 510, 1182
EarI CTCTTC 3 cut(s) 356, 510, 1182
Ecl136II GAGCTC 1 cut(s) 1566
EclXI CGGCCG 1 cut(s) 241
Eco130I CCWWGG 6 cut(s) 54, 186, 234, 978, 1519, 2218
Eco147I AGGCCT 1 cut(s) 1843
Eco24I GRGCYC 1 cut(s) 1568
Eco47I GGWCC 7 cut(s) 183, 664, 1936, 1978, 1999, 2125, 2167
Eco52I CGGCCG 1 cut(s) 241
Eco53kI GAGCTC 1 cut(s) 1566
Eco57I CTGAAG 6 cut(s) 329, 1281, 1666, 1958, 2063, 2105
Eco72I CACGTG 1 cut(s) 1816
Eco81I CCTNAGG 1 cut(s) 2291
Eco88I CYCGRG 2 cut(s) 585, 1254
EcoICRI GAGCTC 1 cut(s) 1566
EcoNI CCTNNNNNAGG 1 cut(s) 1491
EcoO109I RGGNCCY 4 cut(s) 664, 1999, 2125, 2167
EcoRII CCWGG 3 cut(s) 776, 828, 1618
EcoT14I CCWWGG 6 cut(s) 54, 186, 234, 978, 1519, 2218
EcoT38I GRGCYC 1 cut(s) 1568
ErhI CCWWGG 6 cut(s) 54, 186, 234, 978, 1519, 2218
Esp3I CGTCTC 1 cut(s) 423
FaeI CATG 6 cut(s) 238, 581, 1330, 1593, 2222, 2547
FalI AAGNNNNNCTT 2 cut(s) 211, 243
FaqI GGGAC 5 cut(s) 196, 398, 525, 2394, 2434
FatI CATG 6 cut(s) 234, 577, 1326, 1589, 2218, 2543
FauI CCCGC 2 cut(s) 2386, 2610
FbaI TGATCA 2 cut(s) 7, 574
FokI GGATG 2 cut(s) 395, 1029
FriOI GRGCYC 1 cut(s) 1568
FspBI CTAG 3 cut(s) 1839, 2214, 2706
GlaI GCGC 3 cut(s) 117, 277, 1314
HaeII RGCGCY 1 cut(s) 119
HaeIII GGCC 6 cut(s) 96, 243, 269, 1828, 1843, 2570
HapII CCGG 4 cut(s) 636, 1090, 1825, 2358
HgaI GACGC 1 cut(s) 679
HhaI GCGC 3 cut(s) 118, 278, 1315
Hin1I GRCGYC 1 cut(s) 690
Hin1II CATG 6 cut(s) 238, 581, 1330, 1593, 2222, 2547
Hin6I GCGC 3 cut(s) 116, 276, 1313
HinP1I GCGC 3 cut(s) 116, 276, 1313
HincII GTYRAC 6 cut(s) 145, 198, 615, 933, 967, 2011
HindII GTYRAC 6 cut(s) 145, 198, 615, 933, 967, 2011
HpaI GTTAAC 4 cut(s) 145, 615, 967, 2011
HpaII CCGG 4 cut(s) 636, 1090, 1825, 2358
HphI GGTGA 8 cut(s) 406, 1315, 1392, 1490, 1928, 1970, 2159, 2201
Hpy99I CGWCG 1 cut(s) 131
HpyCH4III ACNGT 6 cut(s) 31, 877, 1774, 2159, 2385, 2609
HpyCH4IV ACGT 4 cut(s) 69, 1798, 1815, 2349
HpyF10VI GCNNNNNNNGC 9 cut(s) 71, 275, 284, 1301, 1310, 1447, 2099, 2560, 2627
HpySE526I ACGT 4 cut(s) 69, 1798, 1815, 2349
Hsp92I GRCGYC 1 cut(s) 690
Hsp92II CATG 6 cut(s) 238, 581, 1330, 1593, 2222, 2547
HspAI GCGC 3 cut(s) 116, 276, 1313
Ksp22I TGATCA 2 cut(s) 7, 574
KspAI GTTAAC 4 cut(s) 145, 615, 967, 2011
LguI GCTCTTC 2 cut(s) 356, 510
LmnI GCTCC 6 cut(s) 356, 782, 1028, 1082, 1379, 2466
Lsp1109I GCAGC 7 cut(s) 105, 384, 428, 435, 619, 1093, 2089
LweI GCATC 6 cut(s) 469, 475, 1018, 1201, 2443, 2550
MaeI CTAG 3 cut(s) 1839, 2214, 2706
MaeII ACGT 4 cut(s) 69, 1798, 1815, 2349
MfeI CAATTG 1 cut(s) 2049
MflI RGATCY 4 cut(s) 513, 1573, 2317, 2422
MhlI GDGCHC 5 cut(s) 29, 586, 1568, 2289, 2603
MlyI GAGTC 7 cut(s) 998, 1070, 1102, 1154, 1848, 2018, 2456
MmeI TCCRAC 2 cut(s) 654, 1832
MroXI GAANNNNTTC 2 cut(s) 342, 1754
MslI CAYNNNNRTG 2 cut(s) 1325, 2650
MspA1I CMGCKG 3 cut(s) 375, 448, 2093
MspI CCGG 4 cut(s) 636, 1090, 1825, 2358
MspR9I CCNGG 3 cut(s) 778, 830, 1620
MunI CAATTG 1 cut(s) 2049
Mva1269I GAATGC 1 cut(s) 1645
MvaI CCWGG 3 cut(s) 778, 830, 1620
MvnI CGCG 1 cut(s) 276
MwoI GCNNNNNNNGC 9 cut(s) 71, 275, 284, 1301, 1310, 1447, 2099, 2560, 2627
NcoI CCATGG 2 cut(s) 234, 2218
NlaIII CATG 6 cut(s) 238, 581, 1330, 1593, 2222, 2547
NlaIV GGNNCC 6 cut(s) 184, 515, 583, 1575, 2297, 2424
NmeAIII GCCGAG 1 cut(s) 160
NmuCI GTSAC 7 cut(s) 394, 1270, 1398, 1739, 2021, 2209, 2663
NspI RCATGY 1 cut(s) 2547
NspV TTCGAA 1 cut(s) 1985
PaeI GCATGC 1 cut(s) 2547
PaeR7I CTCGAG 1 cut(s) 1254
PceI AGGCCT 1 cut(s) 1843
PciSI GCTCTTC 2 cut(s) 356, 510
PcsI WCGNNNNNNNCGW 1 cut(s) 132
PctI GAATGC 1 cut(s) 1645
PdmI GAANNNNTTC 2 cut(s) 342, 1754
PfeI GAWTC 7 cut(s) 149, 170, 176, 1780, 1961, 2108, 2150
PflMI CCANNNNNTGG 1 cut(s) 2463
PleI GAGTC 7 cut(s) 998, 1069, 1101, 1154, 1848, 2018, 2455
PmaCI CACGTG 1 cut(s) 1816
PmlI CACGTG 1 cut(s) 1816
PpsI GAGTC 7 cut(s) 998, 1069, 1101, 1154, 1848, 2018, 2455
Ppu21I YACGTR 1 cut(s) 1816
PpuMI RGGWCCY 4 cut(s) 664, 1999, 2125, 2167
PshAI GACNNNNGTC 1 cut(s) 1092
PshBI ATTAAT 1 cut(s) 888
PsiI TTATAA 1 cut(s) 218
Psp124BI GAGCTC 1 cut(s) 1568
Psp5II RGGWCCY 4 cut(s) 664, 1999, 2125, 2167
Psp6I CCWGG 3 cut(s) 776, 828, 1618
PspCI CACGTG 1 cut(s) 1816
PspGI CCWGG 3 cut(s) 776, 828, 1618
PspN4I GGNNCC 6 cut(s) 184, 515, 583, 1575, 2297, 2424
PspPI GGNCC 8 cut(s) 183, 664, 1936, 1978, 1999, 2125, 2167, 2568
PspPPI RGGWCCY 4 cut(s) 664, 1999, 2125, 2167
PstI CTGCAG 1 cut(s) 2266
PsuI RGATCY 4 cut(s) 513, 1573, 2317, 2422
PvuII CAGCTG 2 cut(s) 448, 2093
RsaI GTAC 7 cut(s) 247, 760, 919, 998, 1771, 2676, 2694
RsaNI GTAC 7 cut(s) 246, 759, 918, 997, 1770, 2675, 2693
RseI CAYNNNNRTG 2 cut(s) 1325, 2650
SacI GAGCTC 1 cut(s) 1568
SapI GCTCTTC 2 cut(s) 356, 510
Sau96I GGNCC 8 cut(s) 183, 664, 1936, 1978, 1999, 2125, 2167, 2568
ScaI AGTACT 2 cut(s) 998, 2694
SchI GAGTC 7 cut(s) 998, 1070, 1102, 1154, 1848, 2018, 2456
ScrFI CCNGG 3 cut(s) 778, 830, 1620
SduI GDGCHC 5 cut(s) 29, 586, 1568, 2289, 2603
SfaNI GCATC 6 cut(s) 469, 475, 1018, 1201, 2443, 2550
SfcI CTRYAG 4 cut(s) 360, 822, 1215, 2262
Sfr274I CTCGAG 1 cut(s) 1254
SfuI TTCGAA 1 cut(s) 1985
SinI GGWCC 7 cut(s) 183, 664, 1936, 1978, 1999, 2125, 2167
SlaI CTCGAG 1 cut(s) 1254
SmiMI CAYNNNNRTG 2 cut(s) 1325, 2650
SmlI CTYRAG 7 cut(s) 659, 840, 970, 1254, 1551, 1942, 2203
SmoI CTYRAG 7 cut(s) 659, 840, 970, 1254, 1551, 1942, 2203
SpeI ACTAGT 1 cut(s) 2705
SphI GCATGC 1 cut(s) 2547
SseBI AGGCCT 1 cut(s) 1843
SsiI CCGC 8 cut(s) 132, 240, 266, 375, 948, 1914, 2393, 2617
SspI AATATT 3 cut(s) 1429, 1468, 2416
SspMI CTAG 3 cut(s) 1839, 2214, 2706
SstI GAGCTC 1 cut(s) 1568
StuI AGGCCT 1 cut(s) 1843
StyD4I CCNGG 3 cut(s) 776, 828, 1618
StyI CCWWGG 6 cut(s) 54, 186, 234, 978, 1519, 2218
TaaI ACNGT 6 cut(s) 31, 877, 1774, 2159, 2385, 2609
TaiI ACGT 4 cut(s) 72, 1801, 1818, 2352
TaqI TCGA 5 cut(s) 328, 1012, 1255, 1457, 1985
TatI WGTACW 5 cut(s) 758, 996, 1769, 2674, 2692
TauI GCSGC 3 cut(s) 134, 243, 269
TfiI GAWTC 7 cut(s) 149, 170, 176, 1780, 1961, 2108, 2150
TseFI GTSAC 7 cut(s) 394, 1270, 1398, 1739, 2021, 2209, 2663
TseI GCWGC 7 cut(s) 118, 372, 416, 448, 632, 1106, 2102
Tsp45I GTSAC 7 cut(s) 394, 1270, 1398, 1739, 2021, 2209, 2663
TspDTI ATGAA 8 cut(s) 17, 497, 614, 725, 954, 969, 2270, 2388
TspGWI ACGGA 4 cut(s) 142, 305, 401, 719
Van91I CCANNNNNTGG 1 cut(s) 2463
VneI GTGCAC 2 cut(s) 2285, 2599
VpaK11BI GGWCC 7 cut(s) 183, 664, 1936, 1978, 1999, 2125, 2167
VspI ATTAAT 1 cut(s) 888
XagI CCTNNNNNAGG 1 cut(s) 1491
XapI RAATTY 4 cut(s) 496, 1691, 1832, 2489
XceI RCATGY 1 cut(s) 2547
XhoI CTCGAG 1 cut(s) 1254
XmnI GAANNNNTTC 2 cut(s) 342, 1754
XspI CTAG 3 cut(s) 1839, 2214, 2706
ZrmI AGTACT 2 cut(s) 998, 2694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.