Rh6AG033900

OST-HTH/LOTUS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0000679
Physical Location & Seq
Forward (+)
4301853 .. 4309062
7210 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG033900.1

Sequence Viewer

Length: 3555 bp
ATGGTTCCGGAGCGGTCCGAGTCAGATCCGAGTTCGGCCTGTGACCTCCGACACGATGCGAGTGAAGACCCAATCGACTCGGTCGAGGCCGAGACGAGTCTGATCAATGCCGTCTACCTCGACAGCAAGGCCGAGCTCGTGGCTCAGTCGAAGCGACTCGGCGGCCGGGCGTTAGACCTAGCGACCGCGTCGAAGCTCCTGCATCTCTGCTGCAACTTTGACTCTGTAGAGTGCGCCTCGGCGCTTCTCAGCGGACAGCTCGGAGCGATACCACTCGTGAACGAGTTAGACGAGGCCGGGAAAACGCCGCTACACACGGCGGTGGAGTCTCACGCGTTGCGGTGCGTGGAGGCGTTGCTGAAGAAACACGCTCGTACGGACCTGAGGACGAAGGACGGTGAGGCACAGCTACCGTTGGAGTTGTCGCTTACTGAGGCAAGGTTGGATATATATTGGAATCCGAACGATTTCTCCAAGGAAGAATTTGTCCTTCACATAACTGAAAAGGATTTGAGCATTGTGAGACTTTTGAGTGACAAAACCAAGGAGATCGCTGAGGTGGCTTATTCATACGCTGTTCAAGGGTGCGTAGCTTCTCTGACGGCTCTGCTCATCGTCGCCGCCGAGAAGGTTATCGATTCCGTGTTGGAGTTGCGTAATTCCAAATCGGGGTGCAAGGAGAAGTTGACTATCTACGAATGCCTTATAAGGGAGGCTTTGGCCCTGGGCGGCCATACAACGACGCCGTTGAGGGCGGCCAAAAGGAAGCTCTTGCTCCGGGAGATAGACCTGCTTCAGCTCTTCGGAGCTGTAGTTCAGAGCTGTGGTGGAGACAAGAAGGTGACTTCGCCACTCATCCGTGCAGCTCAGGGAGGAGACGAAGCTGTTATCCAGCTGCTTCTGAAGACAAATATAGATGTCAATGATGCTGATGCAGATGGAAATTCTGCTCTTCATTGGATACTTGCGTCATCGATATTGTCACATCCACAACAGATAAGTATTATATTGCTTCTTCTAGAGCATGGTGCTCTCGTAAGGCAAAGGAATAAAATGGGGTTGACAGCGTTTCATATTGCTGCTGGTAATGGTAATTCAAAGGCCCTTGAGATCCTTCTTGAAGTCCTTAACGTGCGTGGGCAAAAAGCAATAGACCTGGCAACCTCTCAAGATATGCGTGACATACTGCTGAACATAACCACTGTTAGCCTCGTTAATCAGGCCTTCCCCAATCAACATAAATGTACTGCCTTAGAGGTGACAGATGAAGACACCACTACTGAAAGAAATGTTAACTCAATCACCAAGGCAGAAGCTTGTAAGTACTTTGAATCTCATCAAGGATGTGTCAGAGGAGTTCAGGATGGAGCATACCAGAGTCATTCTTATGCTGCGAAACAACTCAAACGCAAAATCTTTGTTGGAGGACTTCCTCCTTCAGTGGACTCTGATGTTTTGGGAAAGTTCTTTGAAGAGTTTGGATCAGTGGAAGATGCTGTAGTTATTGTAACTCAGATAGAAAAGAAGATACAATCTCGAGGCTTTGGTTTTGTCACATTTAGAGAGGAGAAATCTGCTTTAGAAGCCATGCAAGCGAACTACGTCACCATGAGAGGCAAGAAAGTTGAAATAAAAAGTTTGATTCCAAAATTGGTTTTCAGAGCTGAGTTTGAGAAAATGTCACCTCAACAACGAGAACAAGATAAAGATTTTCAACTTCCACTGCAGAAGTCGAACGAGTATATTAGGGAAGGTGATAACCCTGAACAAGGTTCTTGCGCTGATAGATTAGTCCTTGGACAACCAGCGGCATGTTCCATTGAACATCAAGCTCATGTGACCAAGAGCTCTGAGGACCCAAGCACTCCTACATGGCTAAAGGTTTTCAAGAAGTGGTTTCCTGGGTTCTTACAAGGTCTCCTGAAGCACTCAAAGTATGCTCTCTCTTCTCTAAAAGCTGATTTCAGGGCTAAATTTGGGTTAGAACTGGATCATTCATCTGTGGGCTACTCTAAGCTCAGTGACTTCCTGAAATGTTTCTCCGATTTATGTACCGTTGAGATTCTCTCCATATGCAAACGTAGACCACCTAGTCACATGATCATCCGGCCAAATTTTTTTAGGCCTCCACGCCGACTCCTTCATACACTCAGAACACCTCATACTCTGTCATTTTTTGCATTGATTGAGAATGGTGGTGATTCAAAGTGTCTTTCAGACCTCTCAATGGGCATTGGTGGTGAATATTCAAAATGTCTTCAGGATCATTCAAAGTGTTTTCATGACCATTCAGTTGCCGATAGTACTGATTCAAAGTGTCTTCAGAATCTTTCAATTGCAGCGGGTAGTGATACAAATTGTCGTCAGGAACTTTCAGCTGACAATGCTGCTGATTCAAGGTGTCTTCAGGACCTTTCACTGGACTTTGGTGGTGATTCAAAGTGTATCCAGGACCTTTCCATTTGCAATGCTTGTGAAAGGTGTGGCTTAAATGTCACTAGCTATGGGGAAGAGAAATTGTCCTTTGGGTACACTAAAGTGAAGAACTCAAGGTTCCTTCAATTCTTGAAGCCAGATCCTGTACTCCATGGTCCGATTTATCAGGAGAAGCTACATAGAGAGACTAAAGGGACTACTATTATTGCTTGGGTTTTAGACCAAGCACCTGCTGTAATGGCTGGTTTTGATTCCAGGATCACTAAGCATGATGAACTAGTTTCAGATACATCGAAAAAATTTGAAATCATTAGTCCAAAGATACTCGCATTAGGTGCAGGAAGCTATTATGATATTAAGAAGACATTTAATATTATGAAGTACTGCATTCCAAACAAGATTATGAGACATCCTGAAGTCATATTTAAGCTAGGAGAGTATGCTCGTGATAGGCCGGTGAGAAAGAATGTTGATTTGTATACCTGTTTTATTCTTGGTGTGCAGTCTGAGGAACCAACCCTTTATCGACTTGACAGTGACCTTGATCCTCCTTTAGAAAAAGTTAAATATGCATTTCTTAAGGCAGCTCTAACCGATCCCTCTACCGGTGGTATTATAACTGTGGCATATATTGATGATGATGGAATGGAAAGAATGTCCAGCAATACGGTTGTAGAATTGTATATACAATTCTATGACAAGTTCCGTTATCTTGAAGACCGAATTGTGTTTCTATTTTATGATAATGTTCAAAAGAAAAAGCTTCCGTCATTTCTTGGAAAGAAAATCAATGAAAATGCTGTTGAAGAAGTTGGTCATCCTCTGACGCAGTTACATGGAGGGGAAGTTTGTCTAGAAACAGATGGTGAAGGGGTCATTACGTTAGACACTGAGATGCAGATGCAGCGGATGGTATTTAAAAGCAACACAGATGCTATTAATTTCTTCAACAAAGCTATAGCAGTTGATAAAGATCTGTCTTTTTCTTCATCTGCACGTGCCTTTATTAGATTCTATAAAGTTTCCGGCAAGAGGATATACGTTGCCAAACAATCTATTGAACTTTTGGAGTCTTTAGTCATTTTGAGAAATCTACGAAACCCTTTGTACTTCCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 OST-HTH RRM_1

Protein Analysis

1184

Amino Acids

131.82

Weight (kDa)

6.25

Isoelectric Point (pI)

41.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 64 - 127 9.9e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 277 - 348 4.2e-06 Ankyrin repeats (3 copies)
RRM_1 PF00076 472 - 542 1.1e-13 RNA recognition motif
OST-HTH PF12872 632 - 687 1.2e-07 OST-HTH/LOTUS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 707, 3053
AarI CACCTGC 1 cut(s) 2674
AasI GACNNNNNNGTC 1 cut(s) 2091
Acc36I ACCTGC 2 cut(s) 798, 2674
AccBSI CCGCTC 1 cut(s) 13
AccI GTMKAC 3 cut(s) 114, 2083, 2915
AccII CGCG 2 cut(s) 188, 335
AccIII TCCGGA 1 cut(s) 7
AclWI GGATC 9 cut(s) 20, 1105, 1489, 1998, 2271, 2570, 2702, 2975, 3026
AcoI YGGCCR 4 cut(s) 163, 730, 756, 2108
AcsI RAATTY 5 cut(s) 482, 943, 1973, 2113, 2735
AcuI CTGAAG 9 cut(s) 380, 779, 923, 1422, 1943, 2243, 2306, 2390, 2871
AcvI CACGTG 1 cut(s) 3434
AcyI GRCGYC 1 cut(s) 743
AfaI GTAC 9 cut(s) 376, 1246, 1325, 2053, 2305, 2531, 2583, 2819, 3545
AfiI CCNNNNNNNGG 7 cut(s) 669, 709, 1769, 2227, 2419, 3041, 3468
AflII CTTAAG 1 cut(s) 3014
AflIII ACRYGT 1 cut(s) 333
AgeI ACCGGT 1 cut(s) 3041
AhlI ACTAGT 1 cut(s) 2713
AjnI CCWGG 5 cut(s) 723, 1155, 1900, 2448, 2690
AleI CACNNNNGTG 2 cut(s) 320, 2537
AloI GAACNNNNNNTCC 4 cut(s) 455, 487, 798, 830
Alw21I GWGCWC 3 cut(s) 138, 1033, 1850
Alw26I GTCTC 8 cut(s) 86, 333, 517, 825, 870, 1922, 2615, 2836
AlwI GGATC 9 cut(s) 20, 1105, 1489, 1998, 2271, 2570, 2702, 2975, 3026
AlwNI CAGNNNCTG 1 cut(s) 2579
Ama87I CYCGRG 1 cut(s) 1536
Aor13HI TCCGGA 1 cut(s) 7
ApeKI GCWGC 9 cut(s) 210, 863, 895, 1079, 1391, 2339, 2387, 3020, 3340
ApoI RAATTY 5 cut(s) 482, 943, 1973, 2113, 2735
AseI ATTAAT 1 cut(s) 3375
AsiGI ACCGGT 1 cut(s) 3041
Asp700I GAANNNNTTC 2 cut(s) 467, 2036
AspLEI GCGC 3 cut(s) 236, 244, 1781
AspS9I GGNCC 8 cut(s) 15, 379, 721, 1102, 1855, 2410, 2452, 2591
AsuC2I CCSGG 3 cut(s) 167, 298, 779
AvaI CYCGRG 1 cut(s) 1536
AvaII GGWCC 6 cut(s) 15, 379, 1855, 2410, 2452, 2591
AxyI CCTNAGG 1 cut(s) 383
BaeI ACNNNNGTAYC 2 cut(s) 953, 986
BanII GRGCYC 2 cut(s) 138, 1850
BauI CACGAG 3 cut(s) 137, 275, 2880
BbrPI CACGTG 1 cut(s) 3434
BbsI GAAGAC 8 cut(s) 72, 911, 1275, 2249, 2312, 2396, 2804, 3159
Bbv12I GWGCWC 3 cut(s) 138, 1033, 1850
BbvCI CCTCAGC 1 cut(s) 555
BbvI GCAGC 9 cut(s) 197, 875, 882, 1066, 1378, 2351, 2374, 3032, 3352
BccI CCATC 5 cut(s) 932, 1358, 3071, 3293, 3340
BceAI ACGGC 4 cut(s) 95, 333, 618, 730
BcgI CGANNNNNNTGC 2 cut(s) 181, 215
BciT130I CCWGG 5 cut(s) 725, 1157, 1902, 2450, 2692
BciVI GTATCC 2 cut(s) 954, 2456
BclI TGATCA 2 cut(s) 102, 2100
BcnI CCSGG 3 cut(s) 167, 298, 779
BcoDI GTCTC 8 cut(s) 86, 333, 517, 825, 870, 1922, 2615, 2836
BcuI ACTAGT 1 cut(s) 2713
BfaI CTAG 7 cut(s) 179, 1019, 2091, 2499, 2714, 2867, 3290
BfmI CTRYAG 5 cut(s) 225, 810, 1497, 1724, 3393
BfoI RGCGCY 1 cut(s) 245
BfrI CTTAAG 1 cut(s) 3014
BfuAI ACCTGC 2 cut(s) 798, 2674
BfuI GTATCC 2 cut(s) 954, 2456
BglII AGATCT 1 cut(s) 3409
BmcAI AGTACT 3 cut(s) 1325, 2305, 2819
Bme1390I CCNGG 8 cut(s) 167, 298, 725, 779, 1157, 1902, 2450, 2692
Bme18I GGWCC 6 cut(s) 15, 379, 1855, 2410, 2452, 2591
BmeT110I CYCGRG 1 cut(s) 1536
BmgT120I GGNCC 8 cut(s) 15, 379, 721, 1102, 1855, 2410, 2452, 2591
BmiI GGNNCC 4 cut(s) 6, 1857, 2555, 2949
BmrFI CCNGG 8 cut(s) 167, 298, 725, 779, 1157, 1902, 2450, 2692
BmsI GCATC 8 cut(s) 46, 211, 916, 922, 1483, 3321, 3327, 3358
BpiI GAAGAC 8 cut(s) 72, 911, 1275, 2249, 2312, 2396, 2804, 3159
BplI GAGNNNNNCTC 4 cut(s) 221, 253, 2051, 2083
Bpu10I CCTNAGC 2 cut(s) 555, 867
BpuEI CTTGAG 3 cut(s) 1127, 1152, 2533
BpuMI CCSGG 3 cut(s) 167, 298, 779
Bsa29I ATCGAT 2 cut(s) 636, 974
BsaAI YACGTR 1 cut(s) 3434
BsaBI GATNNNNATC 1 cut(s) 3408
BsaHI GRCGYC 1 cut(s) 743
BsaI GGTCTC 1 cut(s) 1922
BsaJI CCNNGG 9 cut(s) 237, 474, 543, 723, 724, 1305, 1795, 1901, 2587
BsaWI WCCGGW 2 cut(s) 7, 3041
Bsc4I CCNNNNNNNGG 7 cut(s) 669, 709, 1769, 2227, 2419, 3041, 3468
Bse118I RCCGGY 2 cut(s) 2890, 3041
Bse1I ACTGG 2 cut(s) 1992, 2424
Bse21I CCTNAGG 1 cut(s) 383
Bse3DI GCAATG 1 cut(s) 2473
Bse8I GATNNNNATC 1 cut(s) 3408
BseAI TCCGGA 1 cut(s) 7
BseBI CCWGG 5 cut(s) 725, 1157, 1902, 2450, 2692
BseCI ATCGAT 2 cut(s) 636, 974
BseDI CCNNGG 9 cut(s) 237, 474, 543, 723, 724, 1305, 1795, 1901, 2587
BseGI GGATG 8 cut(s) 855, 985, 1349, 1369, 2103, 2845, 3253, 3351
BseJI GATNNNNATC 1 cut(s) 3408
BseLI CCNNNNNNNGG 7 cut(s) 669, 709, 1769, 2227, 2419, 3041, 3468
BseMI GCAATG 1 cut(s) 2473
BseNI ACTGG 2 cut(s) 1992, 2424
BseRI GAGGAG 3 cut(s) 888, 1368, 1580
BseX3I CGGCCG 1 cut(s) 163
BseXI GCAGC 9 cut(s) 197, 875, 882, 1066, 1378, 2351, 2374, 3032, 3352
BsgI GTGCAG 4 cut(s) 882, 2793, 2957, 3414
Bsh1236I CGCG 2 cut(s) 188, 335
Bsh1285I CGRYCG 3 cut(s) 84, 166, 186
BshTI ACCGGT 1 cut(s) 3041
BshVI ATCGAT 2 cut(s) 636, 974
BsiEI CGRYCG 3 cut(s) 84, 166, 186
BsiHKAI GWGCWC 3 cut(s) 138, 1033, 1850
BsiHKCI CYCGRG 1 cut(s) 1536
BsiSI CCGG 8 cut(s) 8, 166, 297, 778, 2107, 2891, 3042, 3462
BsiWI CGTACG 1 cut(s) 374
BslFI GGGAC 1 cut(s) 2644
BslI CCNNNNNNNGG 7 cut(s) 669, 709, 1769, 2227, 2419, 3041, 3468
BsmAI GTCTC 8 cut(s) 86, 333, 517, 825, 870, 1922, 2615, 2836
BsmBI CGTCTC 2 cut(s) 86, 870
BsmFI GGGAC 1 cut(s) 2644
BsmI GAATGC 2 cut(s) 704, 2823
Bso31I GGTCTC 1 cut(s) 1922
BsoBI CYCGRG 1 cut(s) 1536
Bsp1286I GDGCHC 3 cut(s) 138, 1033, 1850
Bsp13I TCCGGA 1 cut(s) 7
Bsp19I CCATGG 1 cut(s) 2587
BspDI ATCGAT 2 cut(s) 636, 974
BspEI TCCGGA 1 cut(s) 7
BspFNI CGCG 2 cut(s) 188, 335
BspHI TCATGA 1 cut(s) 2281
BspLI GGNNCC 4 cut(s) 6, 1857, 2555, 2949
BspMAI CTGCAG 1 cut(s) 1728
BspMI ACCTGC 2 cut(s) 798, 2674
BspPI GGATC 9 cut(s) 20, 1105, 1489, 1998, 2271, 2570, 2702, 2975, 3026
BspQI GCTCTTC 2 cut(s) 806, 957
BspTI CTTAAG 1 cut(s) 3014
BspTNI GGTCTC 1 cut(s) 1922
BsrBI CCGCTC 1 cut(s) 13
BsrDI GCAATG 1 cut(s) 2473
BsrFI RCCGGY 2 cut(s) 2890, 3041
BsrI ACTGG 2 cut(s) 1992, 2424
BssAI RCCGGY 2 cut(s) 2890, 3041
BssECI CCNNGG 9 cut(s) 237, 474, 543, 723, 724, 1305, 1795, 1901, 2587
BssNAI GTATAC 1 cut(s) 2916
BssNI GRCGYC 1 cut(s) 743
BssSI CACGAG 3 cut(s) 137, 275, 2880
BssT1I CCWWGG 5 cut(s) 474, 543, 1305, 1795, 2587
Bst1107I GTATAC 1 cut(s) 2916
Bst2BI CACGAG 3 cut(s) 137, 275, 2880
Bst2UI CCWGG 5 cut(s) 725, 1157, 1902, 2450, 2692
Bst4CI ACNGT 7 cut(s) 398, 414, 1204, 2056, 2972, 3058, 3106
Bst6I CTCTTC 5 cut(s) 806, 957, 1467, 1951, 2505
BstACI GRCGYC 1 cut(s) 743
BstAFI CTTAAG 1 cut(s) 3014
BstAPI GCANNNNNTGC 1 cut(s) 2771
BstBAI YACGTR 1 cut(s) 3434
BstC8I GCNNGC 1 cut(s) 1593
BstDSI CCRYGG 1 cut(s) 2587
BstENI CCTNNNNNAGG 1 cut(s) 1767
BstF5I GGATG 8 cut(s) 855, 985, 1349, 1369, 2103, 2845, 3253, 3351
BstFNI CGCG 2 cut(s) 188, 335
BstH2I RGCGCY 1 cut(s) 245
BstHHI GCGC 3 cut(s) 236, 244, 1781
BstMAI GTCTC 8 cut(s) 86, 333, 517, 825, 870, 1922, 2615, 2836
BstMCI CGRYCG 3 cut(s) 84, 166, 186
BstMWI GCNNNNNNNGC 7 cut(s) 560, 1583, 1592, 2384, 2675, 2771, 3340
BstNI CCWGG 5 cut(s) 725, 1157, 1902, 2450, 2692
BstNSI RCATGY 1 cut(s) 1815
BstSCI CCNGG 8 cut(s) 165, 296, 723, 777, 1155, 1900, 2448, 2690
BstSFI CTRYAG 5 cut(s) 225, 810, 1497, 1724, 3393
BstUI CGCG 2 cut(s) 188, 335
BstV1I GCAGC 9 cut(s) 197, 875, 882, 1066, 1378, 2351, 2374, 3032, 3352
BstV2I GAAGAC 8 cut(s) 72, 911, 1275, 2249, 2312, 2396, 2804, 3159
BstX2I RGATCY 4 cut(s) 25, 1110, 2575, 3409
BstYI RGATCY 4 cut(s) 25, 1110, 2575, 3409
BstZ17I GTATAC 1 cut(s) 2916
BstZI CGGCCG 1 cut(s) 163
Bsu15I ATCGAT 2 cut(s) 636, 974
Bsu36I CCTNAGG 1 cut(s) 383
BsuI GTATCC 2 cut(s) 954, 2456
BsuTUI ATCGAT 2 cut(s) 636, 974
BtgI CCRYGG 1 cut(s) 2587
BtsCI GGATG 8 cut(s) 855, 985, 1349, 1369, 2103, 2845, 3253, 3351
BtsI GCAGTG 1 cut(s) 1721
BtsIMutI CAGTG 8 cut(s) 1200, 1446, 1491, 1721, 2026, 2417, 2977, 3324
BveI ACCTGC 2 cut(s) 798, 2674
Cac8I GCNNGC 1 cut(s) 1593
CaiI CAGNNNCTG 1 cut(s) 2579
CciI TCATGA 1 cut(s) 2281
CfoI GCGC 3 cut(s) 236, 244, 1781
Cfr10I RCCGGY 2 cut(s) 2890, 3041
Cfr13I GGNCC 8 cut(s) 15, 379, 721, 1102, 1855, 2410, 2452, 2591
ClaI ATCGAT 2 cut(s) 636, 974
CpoI CGGWCCG 1 cut(s) 15
CseI GACGC 4 cut(s) 177, 751, 957, 3271
Csp6I GTAC 9 cut(s) 375, 1245, 1324, 2052, 2304, 2530, 2582, 2818, 3544
CspAI ACCGGT 1 cut(s) 3041
CspI CGGWCCG 1 cut(s) 15
CviQI GTAC 9 cut(s) 375, 1245, 1324, 2052, 2304, 2530, 2582, 2818, 3544
DraI TTTAAA 1 cut(s) 3355
DrdI GACNNNNNNGTC 1 cut(s) 2091
DseDI GACNNNNNNGTC 1 cut(s) 2091
EaeI YGGCCR 4 cut(s) 163, 730, 756, 2108
EagI CGGCCG 1 cut(s) 163
Eam1104I CTCTTC 5 cut(s) 806, 957, 1467, 1951, 2505
EarI CTCTTC 5 cut(s) 806, 957, 1467, 1951, 2505
Ecl136II GAGCTC 2 cut(s) 136, 1848
EclXI CGGCCG 1 cut(s) 163
Eco130I CCWWGG 5 cut(s) 474, 543, 1305, 1795, 2587
Eco147I AGGCCT 2 cut(s) 1223, 2125
Eco24I GRGCYC 2 cut(s) 138, 1850
Eco31I GGTCTC 1 cut(s) 1922
Eco47I GGWCC 6 cut(s) 15, 379, 1855, 2410, 2452, 2591
Eco52I CGGCCG 1 cut(s) 163
Eco53kI GAGCTC 2 cut(s) 136, 1848
Eco57I CTGAAG 9 cut(s) 380, 779, 923, 1422, 1943, 2243, 2306, 2390, 2871
Eco72I CACGTG 1 cut(s) 3434
Eco81I CCTNAGG 1 cut(s) 383
Eco88I CYCGRG 1 cut(s) 1536
EcoICRI GAGCTC 2 cut(s) 136, 1848
EcoNI CCTNNNNNAGG 1 cut(s) 1767
EcoO109I RGGNCCY 4 cut(s) 1102, 1855, 2410, 2452
EcoRII CCWGG 5 cut(s) 723, 1155, 1900, 2448, 2690
EcoT14I CCWWGG 5 cut(s) 474, 543, 1305, 1795, 2587
EcoT22I ATGCAT 1 cut(s) 3010
EcoT38I GRGCYC 2 cut(s) 138, 1850
ErhI CCWWGG 5 cut(s) 474, 543, 1305, 1795, 2587
Esp3I CGTCTC 2 cut(s) 86, 870
FalI AAGNNNNNCTT 2 cut(s) 700, 732
FaqI GGGAC 1 cut(s) 2644
FauI CCCGC 1 cut(s) 2335
FauNDI CATATG 1 cut(s) 2072
FbaI TGATCA 2 cut(s) 102, 2100
FblI GTMKAC 3 cut(s) 114, 2083, 2915
FokI GGATG 8 cut(s) 842, 972, 1356, 1376, 2090, 2832, 3240, 3358
FriOI GRGCYC 2 cut(s) 138, 1850
FspBI CTAG 7 cut(s) 179, 1019, 2091, 2499, 2714, 2867, 3290
GlaI GCGC 3 cut(s) 235, 243, 1780
HaeII RGCGCY 1 cut(s) 245
HapII CCGG 8 cut(s) 8, 166, 297, 778, 2107, 2891, 3042, 3462
HgaI GACGC 4 cut(s) 177, 751, 957, 3271
HhaI GCGC 3 cut(s) 236, 244, 1781
Hin1I GRCGYC 1 cut(s) 743
Hin6I GCGC 3 cut(s) 234, 242, 1779
HinP1I GCGC 3 cut(s) 234, 242, 1779
HincII GTYRAC 3 cut(s) 687, 1062, 1294
HindII GTYRAC 3 cut(s) 687, 1062, 1294
HindIII AAGCTT 2 cut(s) 1314, 3197
HpaI GTTAAC 1 cut(s) 1294
HpaII CCGG 8 cut(s) 8, 166, 297, 778, 2107, 2891, 3042, 3462
Hpy166II GTNNAC 9 cut(s) 115, 280, 687, 1062, 1294, 1444, 2084, 2532, 2916
Hpy8I GTNNAC 9 cut(s) 115, 280, 687, 1062, 1294, 1444, 2084, 2532, 2916
Hpy99I CGWCG 3 cut(s) 193, 620, 745
HpyCH4III ACNGT 7 cut(s) 398, 414, 1204, 2056, 2972, 3058, 3106
HpyCH4IV ACGT 6 cut(s) 1131, 1602, 2080, 3317, 3433, 3477
HpyF10VI GCNNNNNNNGC 7 cut(s) 560, 1583, 1592, 2384, 2675, 2771, 3340
HpySE526I ACGT 6 cut(s) 1131, 1602, 2080, 3317, 3433, 3477
Hsp92I GRCGYC 1 cut(s) 743
HspAI GCGC 3 cut(s) 234, 242, 1779
Kpn2I TCCGGA 1 cut(s) 7
Ksp22I TGATCA 2 cut(s) 102, 2100
KspAI GTTAAC 1 cut(s) 1294
LguI GCTCTTC 2 cut(s) 806, 957
LmnI GCTCC 6 cut(s) 10, 201, 263, 780, 806, 1367
Lsp1109I GCAGC 9 cut(s) 197, 875, 882, 1066, 1378, 2351, 2374, 3032, 3352
LweI GCATC 8 cut(s) 46, 211, 916, 922, 1483, 3321, 3327, 3358
MaeI CTAG 7 cut(s) 179, 1019, 2091, 2499, 2714, 2867, 3290
MaeII ACGT 6 cut(s) 1131, 1602, 2080, 3317, 3433, 3477
MbiI CCGCTC 1 cut(s) 13
MfeI CAATTG 1 cut(s) 2334
MflI RGATCY 4 cut(s) 25, 1110, 2575, 3409
MhlI GDGCHC 3 cut(s) 138, 1033, 1850
MluI ACGCGT 1 cut(s) 333
MmeI TCCRAC 5 cut(s) 73, 396, 423, 627, 1402
Mph1103I ATGCAT 1 cut(s) 3010
MroI TCCGGA 1 cut(s) 7
MroXI GAANNNNTTC 2 cut(s) 467, 2036
MslI CAYNNNNRTG 4 cut(s) 320, 1386, 2537, 3329
MspA1I CMGCKG 6 cut(s) 252, 895, 1808, 2342, 2378, 3343
MspCI CTTAAG 1 cut(s) 3014
MspI CCGG 8 cut(s) 8, 166, 297, 778, 2107, 2891, 3042, 3462
MspR9I CCNGG 8 cut(s) 167, 298, 725, 779, 1157, 1902, 2450, 2692
MunI CAATTG 1 cut(s) 2334
Mva1269I GAATGC 2 cut(s) 704, 2823
MvaI CCWGG 5 cut(s) 725, 1157, 1902, 2450, 2692
MvnI CGCG 2 cut(s) 188, 335
MwoI GCNNNNNNNGC 7 cut(s) 560, 1583, 1592, 2384, 2675, 2771, 3340
NciI CCSGG 3 cut(s) 167, 298, 779
NcoI CCATGG 1 cut(s) 2587
NdeI CATATG 1 cut(s) 2072
NlaIV GGNNCC 4 cut(s) 6, 1857, 2555, 2949
NmeAIII GCCGAG 5 cut(s) 115, 138, 157, 218, 649
NsiI ATGCAT 1 cut(s) 3010
NspI RCATGY 1 cut(s) 1815
OliI CACNNNNGTG 2 cut(s) 320, 2537
PaeR7I CTCGAG 1 cut(s) 1536
PagI TCATGA 1 cut(s) 2281
PaqCI CACCTGC 1 cut(s) 2674
PasI CCCWGGG 1 cut(s) 724
PceI AGGCCT 2 cut(s) 1223, 2125
PciSI GCTCTTC 2 cut(s) 806, 957
PcsI WCGNNNNNNNCGW 3 cut(s) 81, 288, 621
PctI GAATGC 2 cut(s) 704, 2823
PdmI GAANNNNTTC 2 cut(s) 467, 2036
Pfl23II CGTACG 1 cut(s) 374
PflFI GACNNNGTC 2 cut(s) 80, 187
PfoI TCCNGGA 3 cut(s) 777, 2448, 2690
PinAI ACCGGT 1 cut(s) 3041
PmaCI CACGTG 1 cut(s) 3434
PmlI CACGTG 1 cut(s) 3434
Ppu21I YACGTR 1 cut(s) 3434
PpuMI RGGWCCY 3 cut(s) 1855, 2410, 2452
PshBI ATTAAT 1 cut(s) 3375
PsiI TTATAA 2 cut(s) 707, 3053
Psp124BI GAGCTC 2 cut(s) 138, 1850
Psp5II RGGWCCY 3 cut(s) 1855, 2410, 2452
Psp6I CCWGG 5 cut(s) 723, 1155, 1900, 2448, 2690
PspCI CACGTG 1 cut(s) 3434
PspGI CCWGG 5 cut(s) 723, 1155, 1900, 2448, 2690
PspLI CGTACG 1 cut(s) 374
PspN4I GGNNCC 4 cut(s) 6, 1857, 2555, 2949
PspPI GGNCC 8 cut(s) 15, 379, 721, 1102, 1855, 2410, 2452, 2591
PspPPI RGGWCCY 3 cut(s) 1855, 2410, 2452
PsrI GAACNNNNNNTAC 2 cut(s) 2146, 2178
PstI CTGCAG 1 cut(s) 1728
PstNI CAGNNNCTG 1 cut(s) 2579
PsuI RGATCY 4 cut(s) 25, 1110, 2575, 3409
PsyI GACNNNGTC 2 cut(s) 80, 187
PvuII CAGCTG 2 cut(s) 895, 2378
RsaI GTAC 9 cut(s) 376, 1246, 1325, 2053, 2305, 2531, 2583, 2819, 3545
RsaNI GTAC 9 cut(s) 375, 1245, 1324, 2052, 2304, 2530, 2582, 2818, 3544
RseI CAYNNNNRTG 4 cut(s) 320, 1386, 2537, 3329
Rsr2I CGGWCCG 1 cut(s) 15
RsrII CGGWCCG 1 cut(s) 15
SacI GAGCTC 2 cut(s) 138, 1850
SapI GCTCTTC 2 cut(s) 806, 957
Sau96I GGNCC 8 cut(s) 15, 379, 721, 1102, 1855, 2410, 2452, 2591
ScaI AGTACT 3 cut(s) 1325, 2305, 2819
ScrFI CCNGG 8 cut(s) 167, 298, 725, 779, 1157, 1902, 2450, 2692
SduI GDGCHC 3 cut(s) 138, 1033, 1850
SfaNI GCATC 8 cut(s) 46, 211, 916, 922, 1483, 3321, 3327, 3358
SfcI CTRYAG 5 cut(s) 225, 810, 1497, 1724, 3393
Sfr274I CTCGAG 1 cut(s) 1536
SinI GGWCC 6 cut(s) 15, 379, 1855, 2410, 2452, 2591
SlaI CTCGAG 1 cut(s) 1536
SmiMI CAYNNNNRTG 4 cut(s) 320, 1386, 2537, 3329
SmlI CTYRAG 5 cut(s) 1106, 1167, 1536, 2548, 3014
SmoI CTYRAG 5 cut(s) 1106, 1167, 1536, 2548, 3014
SpeI ACTAGT 1 cut(s) 2713
SseBI AGGCCT 2 cut(s) 1223, 2125
SspI AATATT 2 cut(s) 2246, 2809
SspMI CTAG 7 cut(s) 179, 1019, 2091, 2499, 2714, 2867, 3290
SstI GAGCTC 2 cut(s) 138, 1850
StuI AGGCCT 2 cut(s) 1223, 2125
StyD4I CCNGG 8 cut(s) 165, 296, 723, 777, 1155, 1900, 2448, 2690
StyI CCWWGG 5 cut(s) 474, 543, 1305, 1795, 2587
TaaI ACNGT 7 cut(s) 398, 414, 1204, 2056, 2972, 3058, 3106
TaiI ACGT 6 cut(s) 1134, 1605, 2083, 3320, 3436, 3480
TaqII GACCGA 2 cut(s) 70, 3171
TatI WGTACW 6 cut(s) 1244, 1323, 2303, 2581, 2817, 3543
TauI GCSGC 6 cut(s) 165, 310, 623, 732, 758, 1811
TscAI CASTG 8 cut(s) 1207, 1446, 1491, 1728, 2026, 2424, 2977, 3331
TseI GCWGC 9 cut(s) 210, 863, 895, 1079, 1391, 2339, 2387, 3020, 3340
TspGWI ACGGA 5 cut(s) 392, 631, 848, 3131, 3192
TspRI CASTG 8 cut(s) 1207, 1446, 1491, 1728, 2026, 2424, 2977, 3331
Tth111I GACNNNGTC 2 cut(s) 80, 187
Vha464I CTTAAG 1 cut(s) 3014
VpaK11BI GGWCC 6 cut(s) 15, 379, 1855, 2410, 2452, 2591
VspI ATTAAT 1 cut(s) 3375
XagI CCTNNNNNAGG 1 cut(s) 1767
XapI RAATTY 5 cut(s) 482, 943, 1973, 2113, 2735
XbaI TCTAGA 2 cut(s) 1018, 3289
XceI RCATGY 1 cut(s) 1815
XhoI CTCGAG 1 cut(s) 1536
XmiI GTMKAC 3 cut(s) 114, 2083, 2915
XmnI GAANNNNTTC 2 cut(s) 467, 2036
XspI CTAG 7 cut(s) 179, 1019, 2091, 2499, 2714, 2867, 3290
ZrmI AGTACT 3 cut(s) 1325, 2305, 2819
Zsp2I ATGCAT 1 cut(s) 3010
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.