Prupe.3G268600_v2.0.a1

OST-HTH/LOTUS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0000679
Physical Location & Seq
Reverse (-)
25063720 .. 25070556
6837 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G268600.4

Sequence Viewer

Length: 3348 bp
ATGGATCTGGCAATACAAAGATTCGGAGCCACGGAGCTCTTGATCAACTCGCTTCCCAAATGCACCTATTTCTCTTACAATCGCGATGAAAAGCTCGCTTCGCATGCGGAGGACGATCTCCGAAACGAGACGAGTGAACACTTCGACTTCGTTGAAGCTGAATCGCTCTTGATCAAATCGGTTTTACGAGACAACAAGGATCAACTCGTAACTCACACGATACGACTCGCCGAGGCCTCCACGGTGCCCCTAGACCGAAGCACAGTATCGAAGCTTCTCCACCTCTGCTGTAACTTCGACTCGCCGGACTGCGCCTCCGCATTGCTCGGCGGGGAGCTTGGAACGACGCCGTTGGTGAACGAGTTCGATGACTCGGGAAAATCGGCGCTTCACACAGCGGCTCTGGCTCACGCGGCCAAGTGCGTCGAGGTCTTGCTCAAGAAACACGCTCGTACGGGCCTGAGGACCAGAGACGGACGAGCACAGCTAGCTCTGGACCTGTCTCTGTCTAGCGCAAGGATGGATGTGATTTGGACTCCAGATGAATACACCCTTGAGGATTTGGTCGTCGTTCTCGGCGAAAAGGATTTGACCACCGTGAGACTTCTCAGTGAAAAAACCAAGGAAATCGGTCAGGTGGCCTATGCAAACGCCGTTGAAGGGCGTATAGTTGCTTTAGCGGCTCTGCTTATTGTAGCCGCAGAGAAGATTAGCGAAACGGTATTGGAGTTGCGTGATAACGATTCGGCTTCTAAAGAGAAGGTGACCTTATACGAATGTGTTGTACGGGAAGCTTTGTCCCTGGTCCGTCCAACGACGCGGTTGACGGCGGCGAAACGCACCTCGACGGCCACCGAGAACGACAACTCCGAGAAAAGGAGGCTTTTGCTTCTCGAGATAGAGTTGCTTCAGCTCTTCGGAGCTGTTGCTGAAAGCGGTTGTACGGACAAGAAGGTGACGTCACCACTCATCCGAGCGGCCCAGGTAGGAGATGAAGCCGTCATGCAGCTGCTTCTGCAGACAGATATAGATGTAAATGATGCTGATGCAGAAGGAAATTCTGCTCTTCATTGGACCCTTAAATTGTCGAGATCGTTATGTCCACAGCAGATAAAAATTTTATGGCTTCTCATTAAGCATGGTGCACGAGTAAGCCAAAGGAATAAATTAGGATTAACTGCCTTACATATTGCTGCTGGTAATGGTAATTCAGAGGCACTTCAGGTCCTTCTATTGGAAGCCCCAGATGGTACCCAGTATAAGACAGAAATTAAAGAAACCCCGCTGTTCTTTGCTGTTAGGAATGATAGTATGGAGTGTGCTGAGCTTCTTTTGAGCTGGGGAGCAAGTAGTGAAATTCTCAATCTGCGTAGACAAAGACCTATTGACCTGACAACCTCGCAAGATATGCGTTTCATATTGCTGAACCCAACCAGTGTTAACCTCAGCAACAATGCTTTCCCCAATCAACATAAATGTATTGCTTGTTCACAAGGTGATGAGGCTTTTTCAAGCACCTGCGAAGCACTTCTAACCATGACAGATGAAGACACCACTTCTGAAAGAAAAATTCACTCCAGCACCAAGGTAGAGATTTGCAAATACTTCGTCTCTCCTCGTGGATGTGTCAGAGGAGCTAAGTGCTTTTATGCACATGGTATGGAGGAGCATCAGAAAGTGAAGCAGGAAGCAGTCTGCAATCATTCTCACGATGCTAAAGAAATCAAGCGCATCTTTGTAGGAGGACTTCCTCCTTCAGTGGGATCTGCAGATTCCTTGGGTAAGTTCTTTGAAGAACAATTTGGGCCAGTGGATGATGCCATAGTCATTTTCTCTCAAATAGAAAACAAGATACAATCTCGAGGCTTTGGATTTGTCACATTTAAAAAGGAGAAATCTGTTTCCGCAGCTGTTCAAGCACACTATGTCTCCATGCTAAACAAACCAGTCGAAATAAAAAGTGTGGTACCAAGATTGGCAGCTGAGTCGGAGAAATTGTCACCTCGACAGCAAGGACAAGAGAAGAATTGCCGACCTCAACTTCCACCACAGATGTCGTCCGATGAGATGATTATGGAAGCAAACAAACCTGAACAAGGTTCTTGGCTTGATAAGTTACTCCATGGCCAACCAAAGACAAGTCCCATAAAACCTCGAGCACGTAAAATCTCAAGCCCAGAAGATAAAAGCATGCCTATTTGGCTAAAGGCTTTTAAGAAGTGGTTTCCTGGGTTCTTACAAGATCTGTCAAAGCATCCAAGGAATGGAAAATATGCTCTATCTTCTCTGAAAGGTGATTTCAGGGCTAAATTTGGCTTAGAACTGGATCATGCACCTCTTGGATTCTCCAAGCTGAGTGACTTCATCAAATCTTTCTCCAACTTATGTACCGTTAAGGTTGACCCTGTAGGAAAAAATGGATTTCTCAATCACATGATCCTCCAACCAAAATTCAGGCATCATCACCAACTCTGTCAATGTCATACACTCAGAATGTCTAGCAATTCCTCTGTTTCTACAGCGAGCGATGATGGTGGTAATTCAAAGTGTCTTCAGGACATTTCGACTGATGATGGTGGTGATTCAAAGTGTCTTCAGGACATTTCAACTGATGATGTTGGTGATTCAGAGTGTCTTCAGGACATTTCGACTGATGATGTTGGTGATTCAGAGTGTCTTCAGGACCTTTCAGTTGATGATGGTGGTGATTCAAAGTGTCTTCGGGACCTTCCAATTGATGATGGTGCTGGAAACTTGGGATTAAAGGAAACCAGTAGCTATAGGGAAGAGAAACCCTCCCATGGGCATCATCCTGAAGTGAATTCAGCTAAGGATGCATCACATTGCATCCACCCAAGGGTCCTGCAATTCTTGAAGCCAGATCCTCTGTTCCACGGTAAAAAAGAACTTGATGTGAGCAGTGAAAGAGGACAATGCATAGGAGAGCTTAGAGGAAGCACTAATAATCGGAGAGACCCACAACGGCATCTGGTTTTGGAGACCCTTGCCAGAAAAAGGAACAAATCATTTAGTTACTTCCTTCGTGACTTTGATTTTTACAAGGATTATAAGGAATGCATTTTGGAGGGAAAGTGTTTTGGATGCAACCAGAAGCAGATGTTATGGGCCAATTTTCCATGCCAACACTTGCTGTGGTGCGGCTCCTGTAAAGTACAGGCCATACTGGCTGCTGGTGATTTTGAACACAAATGCGTGGTGTGTGATTTGCAAGTCCACAAAATCATCACACTTCCGCGCCACGATGACTTCCCACCTATTGTTGATCCTGCAGCTTATTTACCAACTCCATTGATGAAGAAGACATCTCCATTAAGTGGTAACAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 OST-HTH RRM_1

Protein Analysis

1116

Amino Acids

123.69

Weight (kDa)

6.25

Isoelectric Point (pI)

42.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3069
AarI CACCTGC 1 cut(s) 1526
AatII GACGTC 1 cut(s) 962
Acc36I ACCTGC 1 cut(s) 1526
Acc65I GGTACC 2 cut(s) 1250, 1966
AccB1I GGYRCC 3 cut(s) 244, 1250, 1966
AccB7I CCANNNNNTGG 2 cut(s) 2264, 3335
AccBSI CCGCTC 1 cut(s) 977
AccI GTMKAC 1 cut(s) 1372
AccII CGCG 4 cut(s) 84, 413, 820, 3256
AclWI GGATC 7 cut(s) 12, 207, 1771, 2334, 2431, 2876, 3278
AcoI YGGCCR 3 cut(s) 414, 849, 2125
AcsI RAATTY 7 cut(s) 1057, 1116, 1356, 1569, 2309, 2450, 2821
AcuI CTGAAG 8 cut(s) 893, 1205, 1740, 2537, 2579, 2621, 2663, 2835
AcyI GRCGYC 2 cut(s) 347, 959
AdeI CACNNNGTG 1 cut(s) 1496
AfaI GTAC 7 cut(s) 454, 786, 943, 1252, 1968, 2389, 3174
AjnI CCWGG 3 cut(s) 801, 981, 2227
AjuI GAANNNNNNNTTGG 2 cut(s) 1785, 1817
AloI GAACNNNNNNTCC 2 cut(s) 851, 883
Alw21I GWGCWC 4 cut(s) 39, 484, 1147, 2161
Alw26I GTCTC 9 cut(s) 122, 183, 465, 507, 595, 1615, 1933, 2967, 2993
Alw44I GTGCAC 1 cut(s) 1143
AlwI GGATC 7 cut(s) 12, 207, 1771, 2334, 2431, 2876, 3278
Ama87I CYCGRG 4 cut(s) 373, 893, 1860, 2154
ApaLI GTGCAC 1 cut(s) 1143
ApeKI GCWGC 7 cut(s) 1006, 1009, 1193, 1907, 1979, 3188, 3290
ApoI RAATTY 7 cut(s) 1057, 1116, 1356, 1569, 2309, 2450, 2821
ArsI GACNNNNNNTTYG 2 cut(s) 1593, 1625
Asp700I GAANNNNTTC 2 cut(s) 362, 1527
Asp718I GGTACC 2 cut(s) 1250, 1966
AspLEI GCGC 5 cut(s) 314, 388, 515, 1731, 3258
AsuNHI GCTAGC 1 cut(s) 487
AvaI CYCGRG 4 cut(s) 373, 893, 1860, 2154
AvaII GGWCC 8 cut(s) 465, 496, 805, 1074, 1225, 2683, 2725, 2860
AxyI CCTNAGG 1 cut(s) 461
BaeGI GKGCMC 2 cut(s) 249, 1147
BaeI ACNNNNGTAYC 2 cut(s) 2371, 2404
BalI TGGCCA 1 cut(s) 2127
BanI GGYRCC 3 cut(s) 244, 1250, 1966
BanII GRGCYC 1 cut(s) 39
BarI GAAGNNNNNNTAC 4 cut(s) 258, 290, 1731, 1763
BauI CACGAG 2 cut(s) 1146, 1617
BbsI GAAGAC 7 cut(s) 1554, 2543, 2585, 2627, 2669, 2711, 3326
Bbv12I GWGCWC 4 cut(s) 39, 484, 1147, 2161
BbvCI CCTCAGC 1 cut(s) 1445
BbvI GCAGC 7 cut(s) 996, 1018, 1180, 1919, 1991, 3175, 3302
BccI CCATC 6 cut(s) 514, 1241, 2525, 2567, 2693, 2735
BceAI ACGGC 6 cut(s) 334, 638, 843, 864, 983, 2999
BcgI CGANNNNNNTGC 4 cut(s) 1588, 1622, 1968, 2002
BciT130I CCWGG 3 cut(s) 803, 983, 2229
BclI TGATCA 2 cut(s) 42, 171
BcoDI GTCTC 9 cut(s) 122, 183, 465, 507, 595, 1615, 1933, 2967, 2993
BfaI CTAG 4 cut(s) 251, 488, 510, 2499
BfmI CTRYAG 6 cut(s) 1016, 1767, 2406, 2517, 2779, 3288
BfoI RGCGCY 1 cut(s) 389
BfuAI ACCTGC 1 cut(s) 1526
BglI GCCNNNNNGGC 2 cut(s) 2200, 3185
BglII AGATCT 1 cut(s) 2242
BlpI GCTNAGC 1 cut(s) 1323
Bme1390I CCNGG 3 cut(s) 803, 983, 2229
Bme18I GGWCC 8 cut(s) 465, 496, 805, 1074, 1225, 2683, 2725, 2860
BmeT110I CYCGRG 4 cut(s) 373, 893, 1860, 2154
BmiI GGNNCC 8 cut(s) 28, 246, 1076, 1252, 1968, 2726, 2861, 3163
BmrFI CCNGG 3 cut(s) 803, 983, 2229
BmrI ACTGGG 1 cut(s) 1249
BmtI GCTAGC 1 cut(s) 491
BmuI ACTGGG 1 cut(s) 1249
BpiI GAAGAC 7 cut(s) 1554, 2543, 2585, 2627, 2669, 2711, 3326
BplI GAGNNNNNCTC 2 cut(s) 2780, 2812
BpmI CTGGAG 2 cut(s) 522, 1561
Bpu10I CCTNAGC 2 cut(s) 1445, 2829
Bpu1102I GCTNAGC 1 cut(s) 1323
BpuEI CTTGAG 3 cut(s) 422, 575, 2155
BsaAI YACGTR 1 cut(s) 2162
BsaHI GRCGYC 2 cut(s) 347, 959
BsaI GGTCTC 2 cut(s) 2967, 2993
Bse1I ACTGG 7 cut(s) 1255, 1434, 1808, 1946, 2328, 2772, 3189
Bse21I CCTNAGG 1 cut(s) 461
Bse3DI GCAATG 2 cut(s) 320, 2842
BseBI CCWGG 3 cut(s) 803, 983, 2229
BseMI GCAATG 2 cut(s) 320, 2842
BseMII CTCAG 7 cut(s) 452, 622, 1314, 1459, 1974, 2345, 2503
BseNI ACTGG 7 cut(s) 1255, 1434, 1808, 1946, 2328, 2772, 3189
BseRI GAGGAG 3 cut(s) 1605, 1647, 1679
BseSI GKGCMC 2 cut(s) 249, 1147
BseXI GCAGC 7 cut(s) 996, 1018, 1180, 1919, 1991, 3175, 3302
BseYI CCCAGC 1 cut(s) 1338
Bsh1236I CGCG 4 cut(s) 84, 413, 820, 3256
BshNI GGYRCC 3 cut(s) 244, 1250, 1966
BsiHKAI GWGCWC 4 cut(s) 39, 484, 1147, 2161
BsiHKCI CYCGRG 4 cut(s) 373, 893, 1860, 2154
BsiSI CCGG 1 cut(s) 305
BsiWI CGTACG 1 cut(s) 452
BslFI GGGAC 3 cut(s) 784, 2127, 2738
BsmAI GTCTC 9 cut(s) 122, 183, 465, 507, 595, 1615, 1933, 2967, 2993
BsmBI CGTCTC 3 cut(s) 122, 465, 1615
BsmFI GGGAC 3 cut(s) 784, 2127, 2738
BsmI GAATGC 1 cut(s) 3080
Bso31I GGTCTC 2 cut(s) 2967, 2993
BsoBI CYCGRG 4 cut(s) 373, 893, 1860, 2154
Bsp1286I GDGCHC 5 cut(s) 39, 249, 484, 1147, 2161
Bsp1720I GCTNAGC 1 cut(s) 1323
Bsp19I CCATGG 2 cut(s) 2122, 2800
Bsp68I TCGCGA 1 cut(s) 84
BspCNI CTCAG 7 cut(s) 453, 621, 1315, 1458, 1975, 2346, 2502
BspFNI CGCG 4 cut(s) 84, 413, 820, 3256
BspLI GGNNCC 8 cut(s) 28, 246, 1076, 1252, 1968, 2726, 2861, 3163
BspMAI CTGCAG 3 cut(s) 1020, 1771, 3292
BspMI ACCTGC 1 cut(s) 1526
BspOI GCTAGC 1 cut(s) 491
BspPI GGATC 7 cut(s) 12, 207, 1771, 2334, 2431, 2876, 3278
BspQI GCTCTTC 2 cut(s) 920, 1071
BspT107I GGYRCC 3 cut(s) 244, 1250, 1966
BspTNI GGTCTC 2 cut(s) 2967, 2993
BsrBI CCGCTC 1 cut(s) 977
BsrDI GCAATG 2 cut(s) 320, 2842
BsrI ACTGG 7 cut(s) 1255, 1434, 1808, 1946, 2328, 2772, 3189
BssNI GRCGYC 2 cut(s) 347, 959
BssSI CACGAG 2 cut(s) 1146, 1617
BssT1I CCWWGG 7 cut(s) 621, 1584, 1776, 2122, 2258, 2800, 2855
Bst2BI CACGAG 2 cut(s) 1146, 1617
Bst2UI CCWGG 3 cut(s) 803, 983, 2229
Bst4CI ACNGT 6 cut(s) 244, 265, 598, 721, 2392, 2897
Bst6I CTCTTC 3 cut(s) 920, 1071, 2781
BstACI GRCGYC 2 cut(s) 347, 959
BstAPI GCANNNNNTGC 1 cut(s) 1408
BstBAI YACGTR 1 cut(s) 2162
BstC8I GCNNGC 5 cut(s) 96, 105, 489, 2192, 2524
BstDSI CCRYGG 5 cut(s) 30, 240, 2122, 2800, 2893
BstEII GGTNACC 1 cut(s) 763
BstENI CCTNNNNNAGG 1 cut(s) 2094
BstFNI CGCG 4 cut(s) 84, 413, 820, 3256
BstH2I RGCGCY 1 cut(s) 389
BstHHI GCGC 5 cut(s) 314, 388, 515, 1731, 3258
BstMAI GTCTC 9 cut(s) 122, 183, 465, 507, 595, 1615, 1933, 2967, 2993
BstNI CCWGG 3 cut(s) 803, 983, 2229
BstNSI RCATGY 2 cut(s) 107, 2194
BstPI GGTNACC 1 cut(s) 763
BstSCI CCNGG 3 cut(s) 801, 981, 2227
BstSFI CTRYAG 6 cut(s) 1016, 1767, 2406, 2517, 2779, 3288
BstSLI GKGCMC 2 cut(s) 249, 1147
BstUI CGCG 4 cut(s) 84, 413, 820, 3256
BstV1I GCAGC 7 cut(s) 996, 1018, 1180, 1919, 1991, 3175, 3302
BstV2I GAAGAC 7 cut(s) 1554, 2543, 2585, 2627, 2669, 2711, 3326
BstX2I RGATCY 4 cut(s) 4, 1763, 2242, 2881
BstYI RGATCY 4 cut(s) 4, 1763, 2242, 2881
Bsu36I CCTNAGG 1 cut(s) 461
BtgI CCRYGG 5 cut(s) 30, 240, 2122, 2800, 2893
BtgZI GCGATG 2 cut(s) 99, 2541
BtsI GCAGTG 1 cut(s) 2926
BtsIMutI CAGTG 5 cut(s) 616, 1441, 1764, 1815, 2926
BtuMI TCGCGA 1 cut(s) 84
BveI ACCTGC 1 cut(s) 1526
Cac8I GCNNGC 5 cut(s) 96, 105, 489, 2192, 2524
CfoI GCGC 5 cut(s) 314, 388, 515, 1731, 3258
CseI GACGC 3 cut(s) 355, 412, 826
Csp6I GTAC 7 cut(s) 453, 785, 942, 1251, 1967, 2388, 3173
CviQI GTAC 7 cut(s) 453, 785, 942, 1251, 1967, 2388, 3173
DraI TTTAAA 1 cut(s) 1885
DraIII CACNNNGTG 1 cut(s) 1496
EaeI YGGCCR 3 cut(s) 414, 849, 2125
Eam1104I CTCTTC 3 cut(s) 920, 1071, 2781
EarI CTCTTC 3 cut(s) 920, 1071, 2781
Ecl136II GAGCTC 1 cut(s) 37
Eco130I CCWWGG 7 cut(s) 621, 1584, 1776, 2122, 2258, 2800, 2855
Eco147I AGGCCT 1 cut(s) 236
Eco24I GRGCYC 1 cut(s) 39
Eco31I GGTCTC 2 cut(s) 2967, 2993
Eco47I GGWCC 8 cut(s) 465, 496, 805, 1074, 1225, 2683, 2725, 2860
Eco53kI GAGCTC 1 cut(s) 37
Eco57I CTGAAG 8 cut(s) 893, 1205, 1740, 2537, 2579, 2621, 2663, 2835
Eco81I CCTNAGG 1 cut(s) 461
Eco88I CYCGRG 4 cut(s) 373, 893, 1860, 2154
Eco91I GGTNACC 1 cut(s) 763
EcoICRI GAGCTC 1 cut(s) 37
EcoNI CCTNNNNNAGG 1 cut(s) 2094
EcoO109I RGGNCCY 4 cut(s) 1225, 2683, 2725, 2860
EcoO65I GGTNACC 1 cut(s) 763
EcoRI GAATTC 1 cut(s) 2821
EcoRII CCWGG 3 cut(s) 801, 981, 2227
EcoT14I CCWWGG 7 cut(s) 621, 1584, 1776, 2122, 2258, 2800, 2855
EcoT22I ATGCAT 3 cut(s) 2839, 2939, 3080
EcoT38I GRGCYC 1 cut(s) 39
ErhI CCWWGG 7 cut(s) 621, 1584, 1776, 2122, 2258, 2800, 2855
Esp3I CGTCTC 3 cut(s) 122, 465, 1615
FalI AAGNNNNNCTT 4 cut(s) 752, 784, 1718, 1750
FaqI GGGAC 3 cut(s) 784, 2127, 2738
FauI CCCGC 2 cut(s) 323, 1290
FbaI TGATCA 2 cut(s) 42, 171
FblI GTMKAC 1 cut(s) 1372
FriOI GRGCYC 1 cut(s) 39
FspBI CTAG 4 cut(s) 251, 488, 510, 2499
GlaI GCGC 5 cut(s) 313, 387, 514, 1730, 3257
GsaI CCCAGC 1 cut(s) 1342
GsuI CTGGAG 2 cut(s) 522, 1561
HaeII RGCGCY 1 cut(s) 389
HapII CCGG 1 cut(s) 305
HgaI GACGC 3 cut(s) 355, 412, 826
HhaI GCGC 5 cut(s) 314, 388, 515, 1731, 3258
Hin1I GRCGYC 2 cut(s) 347, 959
Hin6I GCGC 5 cut(s) 312, 386, 513, 1729, 3256
HinP1I GCGC 5 cut(s) 312, 386, 513, 1729, 3256
HincII GTYRAC 3 cut(s) 825, 1441, 2401
HindII GTYRAC 3 cut(s) 825, 1441, 2401
HindIII AAGCTT 2 cut(s) 272, 792
HpaI GTTAAC 1 cut(s) 1441
HpaII CCGG 1 cut(s) 305
Hpy99I CGWCG 5 cut(s) 349, 428, 572, 820, 850
HpyAV CCTTC 8 cut(s) 653, 754, 946, 1046, 1238, 1764, 2738, 3050
HpyCH4III ACNGT 6 cut(s) 244, 265, 598, 721, 2392, 2897
HpyCH4IV ACGT 2 cut(s) 959, 2161
HpySE526I ACGT 2 cut(s) 959, 2161
Hsp92I GRCGYC 2 cut(s) 347, 959
HspAI GCGC 5 cut(s) 312, 386, 513, 1729, 3256
KpnI GGTACC 2 cut(s) 1254, 1970
Ksp22I TGATCA 2 cut(s) 42, 171
KspAI GTTAAC 1 cut(s) 1441
LguI GCTCTTC 2 cut(s) 920, 1071
LmnI GCTCC 8 cut(s) 26, 34, 334, 920, 1343, 1634, 1666, 3167
Lsp1109I GCAGC 7 cut(s) 996, 1018, 1180, 1919, 1991, 3175, 3302
MaeI CTAG 4 cut(s) 251, 488, 510, 2499
MaeII ACGT 2 cut(s) 959, 2161
MbiI CCGCTC 1 cut(s) 977
MfeI CAATTG 1 cut(s) 2733
MflI RGATCY 4 cut(s) 4, 1763, 2242, 2881
MhlI GDGCHC 5 cut(s) 39, 249, 484, 1147, 2161
MlsI TGGCCA 1 cut(s) 2127
MluNI TGGCCA 1 cut(s) 2127
MlyI GAGTC 5 cut(s) 219, 293, 365, 529, 1994
MmeI TCCRAC 4 cut(s) 836, 1968, 2403, 2467
Mox20I TGGCCA 1 cut(s) 2127
Mph1103I ATGCAT 3 cut(s) 2839, 2939, 3080
MroXI GAANNNNTTC 2 cut(s) 362, 1527
MscI TGGCCA 1 cut(s) 2127
Msp20I TGGCCA 1 cut(s) 2127
MspA1I CMGCKG 5 cut(s) 398, 1009, 1285, 1910, 1982
MspI CCGG 1 cut(s) 305
MspR9I CCNGG 3 cut(s) 803, 983, 2229
MunI CAATTG 1 cut(s) 2733
Mva1269I GAATGC 1 cut(s) 3080
MvaI CCWGG 3 cut(s) 803, 983, 2229
MvnI CGCG 4 cut(s) 84, 413, 820, 3256
NcoI CCATGG 2 cut(s) 2122, 2800
NheI GCTAGC 1 cut(s) 487
NlaIV GGNNCC 8 cut(s) 28, 246, 1076, 1252, 1968, 2726, 2861, 3163
NmeAIII GCCGAG 3 cut(s) 256, 306, 555
NmuCI GTSAC 7 cut(s) 763, 955, 960, 1876, 1998, 2357, 3044
NruI TCGCGA 1 cut(s) 84
NsiI ATGCAT 3 cut(s) 2839, 2939, 3080
NspI RCATGY 2 cut(s) 107, 2194
PaeI GCATGC 2 cut(s) 107, 2194
PaeR7I CTCGAG 3 cut(s) 893, 1860, 2154
PaqCI CACCTGC 1 cut(s) 1526
PceI AGGCCT 1 cut(s) 236
PciSI GCTCTTC 2 cut(s) 920, 1071
PcsI WCGNNNNNNNCGW 2 cut(s) 576, 867
PctI GAATGC 1 cut(s) 3080
PdmI GAANNNNTTC 2 cut(s) 362, 1527
PfeI GAWTC 9 cut(s) 21, 161, 743, 1772, 2343, 2582, 2624, 2666, 2708
Pfl23II CGTACG 1 cut(s) 452
PflMI CCANNNNNTGG 2 cut(s) 2264, 3335
PleI GAGTC 5 cut(s) 219, 293, 365, 529, 1993
PpsI GAGTC 5 cut(s) 219, 293, 365, 529, 1993
Ppu21I YACGTR 1 cut(s) 2162
PpuMI RGGWCCY 4 cut(s) 1225, 2683, 2725, 2860
PsiI TTATAA 1 cut(s) 3069
Psp124BI GAGCTC 1 cut(s) 39
Psp5II RGGWCCY 4 cut(s) 1225, 2683, 2725, 2860
Psp6I CCWGG 3 cut(s) 801, 981, 2227
PspEI GGTNACC 1 cut(s) 763
PspFI CCCAGC 1 cut(s) 1338
PspGI CCWGG 3 cut(s) 801, 981, 2227
PspLI CGTACG 1 cut(s) 452
PspN4I GGNNCC 8 cut(s) 28, 246, 1076, 1252, 1968, 2726, 2861, 3163
PspPPI RGGWCCY 4 cut(s) 1225, 2683, 2725, 2860
PspXI VCTCGAGB 1 cut(s) 2154
PsrI GAACNNNNNNTAC 2 cut(s) 1471, 1503
PstI CTGCAG 3 cut(s) 1020, 1771, 3292
PsuI RGATCY 4 cut(s) 4, 1763, 2242, 2881
PvuII CAGCTG 3 cut(s) 1009, 1910, 1982
RruI TCGCGA 1 cut(s) 84
RsaI GTAC 7 cut(s) 454, 786, 943, 1252, 1968, 2389, 3174
RsaNI GTAC 7 cut(s) 453, 785, 942, 1251, 1967, 2388, 3173
SacI GAGCTC 1 cut(s) 39
SapI GCTCTTC 2 cut(s) 920, 1071
SchI GAGTC 5 cut(s) 219, 293, 365, 529, 1994
ScrFI CCNGG 3 cut(s) 803, 983, 2229
SduI GDGCHC 5 cut(s) 39, 249, 484, 1147, 2161
SfcI CTRYAG 6 cut(s) 1016, 1767, 2406, 2517, 2779, 3288
Sfr274I CTCGAG 3 cut(s) 893, 1860, 2154
SinI GGWCC 8 cut(s) 465, 496, 805, 1074, 1225, 2683, 2725, 2860
SlaI CTCGAG 3 cut(s) 893, 1860, 2154
SmlI CTYRAG 6 cut(s) 437, 554, 893, 1860, 2154, 2170
SmoI CTYRAG 6 cut(s) 437, 554, 893, 1860, 2154, 2170
SphI GCATGC 2 cut(s) 107, 2194
SseBI AGGCCT 1 cut(s) 236
SspMI CTAG 4 cut(s) 251, 488, 510, 2499
SstI GAGCTC 1 cut(s) 39
StuI AGGCCT 1 cut(s) 236
StyD4I CCNGG 3 cut(s) 801, 981, 2227
StyI CCWWGG 7 cut(s) 621, 1584, 1776, 2122, 2258, 2800, 2855
TaaI ACNGT 6 cut(s) 244, 265, 598, 721, 2392, 2897
TaiI ACGT 2 cut(s) 962, 2164
TaqII GACCGA 2 cut(s) 270, 620
TatI WGTACW 1 cut(s) 3172
TauI GCSGC 7 cut(s) 401, 416, 683, 701, 833, 980, 3162
TfiI GAWTC 9 cut(s) 21, 161, 743, 1772, 2343, 2582, 2624, 2666, 2708
TscAI CASTG 5 cut(s) 616, 1441, 1764, 1815, 2926
TseFI GTSAC 7 cut(s) 763, 955, 960, 1876, 1998, 2357, 3044
TseI GCWGC 7 cut(s) 1006, 1009, 1193, 1907, 1979, 3188, 3290
Tsp45I GTSAC 7 cut(s) 763, 955, 960, 1876, 1998, 2357, 3044
TspDTI ATGAA 8 cut(s) 102, 558, 1008, 1058, 1405, 1560, 2353, 3329
TspGWI ACGGA 4 cut(s) 47, 489, 797, 959
TspRI CASTG 5 cut(s) 616, 1441, 1764, 1815, 2926
Van91I CCANNNNNTGG 2 cut(s) 2264, 3335
VneI GTGCAC 1 cut(s) 1143
VpaK11BI GGWCC 8 cut(s) 465, 496, 805, 1074, 1225, 2683, 2725, 2860
XagI CCTNNNNNAGG 1 cut(s) 2094
XapI RAATTY 7 cut(s) 1057, 1116, 1356, 1569, 2309, 2450, 2821
XceI RCATGY 2 cut(s) 107, 2194
XhoI CTCGAG 3 cut(s) 893, 1860, 2154
XmiI GTMKAC 1 cut(s) 1372
XmnI GAANNNNTTC 2 cut(s) 362, 1527
XspI CTAG 4 cut(s) 251, 488, 510, 2499
ZraI GACGTC 1 cut(s) 960
Zsp2I ATGCAT 3 cut(s) 2839, 2939, 3080
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.